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https://www.readbyqxmd.com/read/29775757/molecular-characterization-of-physis-tissue-by-rna-sequencing
#1
Christopher R Paradise, Catalina Galeano-Garces, Daniela Galeano-Garces, Amel Dudakovic, Todd A Milbrandt, Daniel B F Saris, Aaron J Krych, Marcel Karperien, Gabriel B Ferguson, Denis Evseenko, Scott M Riester, Andre J van Wijnen, A Noelle Larson
The physis is a well-established and anatomically distinct cartilaginous structure that is crucial for normal long-bone development and growth. Abnormalities in physis function are linked to growth plate disorders and other pediatric musculoskeletal diseases. Understanding the molecular pathways operative in the physis may permit development of regenerative therapies to complement surgically-based procedures that are the current standard of care for growth plate disorders. Here, we performed next generation RNA sequencing on mRNA isolated from human physis and other skeletal tissues (e...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29774468/salivary-anti-coxsackievirus-b4-neutralizing-activity-and-pattern-of-immune-parameters-in-patients-with-type-1-diabetes-a-pilot-study
#2
Magloire Pandoua Nekoua, Akadiri Yessoufou, Enagnon Kazali Alidjinou, Francis Badia-Boungou, Kabirou Moutairou, Famara Sane, Didier Hober
AIMS: Enteroviruses, especially coxsackieviruses B (CV-B), have been associated with the pathogenesis of type 1 diabetes (T1D). An anti-CV-B4 neutralizing activity in saliva of T1D patients was previously reported. Our aim was to study the association between the saliva anti-CV-B4 neutralizing activity and immune parameters in T1D patients in comparison with non-diabetic individuals. METHODS: Saliva and blood samples were collected from 15 T1D patients and 8 controls...
May 17, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29774031/il-10-producing-b-cells-ability-to-induce-regulatory-t-cells-is-maintained-in-rheumatoid-arthritis
#3
Julie Mielle, Rachel Audo, Michael Hahne, Laurence Macia, Bernard Combe, Jacques Morel, Claire Daien
Despite growing evidence highlighting the relevance of increasing IL-10-producing B cells (B10+ cells) in autoimmune diseases, their functions in patients are still unknown. The aim of this study was to evaluate the functions of CpG-induced B10+ cells isolated from healthy controls (HC) and rheumatoid arthritis (RA) patients, on naïve T cell differentiation. We demonstrated that CpG-induced B10+ cells from HC drove naïve T cell differentiation toward regulatory T cells (Treg cells) and IL-10-producing T cells (Tr1) through IL-10 secretion and cellular contacts...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29774025/co-stimulation-impaired-bone-marrow-derived-dendritic-cells-prevent-dextran-sodium-sulfate-induced-colitis-in-mice
#4
Carl Engman, Yesica Garciafigueroa, Brett Eugene Phillips, Massimo Trucco, Nick Giannoukakis
Dendritic cells (DC) are important in the onset and severity of inflammatory bowel disease (IBD). Tolerogenic DC induce T-cells to become therapeutic Foxp3+ regulatory T-cells (Tregs). We therefore asked if experimental IBD could be prevented by administration of bone marrow-derived DC generated under conventional GM-CSF/IL-4 conditions but in the presence of a mixture of antisense DNA oligonucleotides targeting the primary transcripts of CD40, CD80, and CD86. These cell products (which we call AS-ODN BM-DC) have demonstrated tolerogenic activity in preventing type 1 diabetes and preserving beta cell mass in new-onset type 1 diabetes in the NOD mouse strain, in earlier studies...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29773654/genetic-code-expansion-and-live-cell-imaging-reveal-that-thr308-phosphorylation-is-irreplaceable-and-sufficient-for-akt1-activity
#5
Nileeka Balasuriya, Maya T Kunkel, Xuguang Liu, Kyle K Biggar, Shawn S-C Li, Alexandra C Newton, Patrick O'Donoghue
The proto-oncogene Akt/protein kinase B (PKB) is a pivotal signal transducer for growth and survival. Growth factor stimulation leads to Akt phosphorylation at two regulatory sites (Thr308, Ser473), acutely activating Akt signaling. Delineating the exact role of each regulatory site is, however, technically challenging and has remained elusive. Here, we used genetic code expansion to produce site-specifically phosphorylated Akt1 in order to dissect the contribution of each regulatory site to Akt1 activity. We achieved recombinant production of full length Akt1 containing site-specific pThr and pSer residues for the first time...
May 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29771416/long-non-coding-rna-duxap8-regulates-proliferation-and-invasion-of-esophageal-squamous-cell-cancer
#6
L-J Xu, X-J Yu, B Wei, H-X Hui, Y Sun, J Dai, X-F Chen
OBJECTIVE: The purpose of this research was to detect the expression of long non-coding RNA DUXAP8 in esophageal cancer, and to explore its underlying mechanism in the development of esophageal squamous cell carcinoma (ESCC). PATIENTS AND METHODS: We collected 78 pairs of esophageal cancer tissues and normal adjacent tissues. The mRNA level of DUXAP8 in these esophageal cancer tissues and corresponding adjacent tissues was detected by quantitative Real-time polymerase chain reaction (qRT-PCR)...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29770276/the-evaluation-of-anoxia-responsive-e2f-dna-binding-activity-in-the-red-eared-slider-turtle-trachemys-scripta-elegans
#7
Kyle K Biggar, Kenneth B Storey
In many cases, the DNA-binding activity of a transcription factor does not change, while its transcriptional activity is greatly influenced by the make-up of bound proteins. In this study, we assessed the protein composition and DNA-binding ability of the E2F transcription factor complex to provide insight into cell cycle control in an anoxia tolerant turtle through the use of a modified ELISA protocol. This modification also permits the use of custom DNA probes that are tailored to a specific DNA binding region, introducing the ability to design capture probes for non-model organisms...
2018: PeerJ
https://www.readbyqxmd.com/read/29770133/peripheral-blood-stem-cell-mobilization-in-healthy-donors-by-granulocyte-colony-stimulating-factor-causes-preferential-mobilization-of-lymphocyte-subsets
#8
Guro Kristin Melve, Elisabeth Ersvaer, Geir Egil Eide, Einar K Kristoffersen, Øystein Bruserud
Background: Allogeneic hematopoietic stem cell transplantation is associated with a high risk of immune-mediated post-transplant complications. Graft depletion of immunocompetent cell subsets is regarded as a possible strategy to reduce this risk without reducing antileukemic immune reactivity. Study design and methods: We investigated the effect of hematopoietic stem cell mobilization with granulocyte colony-stimulating factor (G-CSF) on peripheral blood and stem cell graft levels of various T, B, and NK cell subsets in healthy donors...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29769415/upregulation-of-microrna-340-promotes-osteosarcoma-cell-apoptosis-while-suppressing-proliferation-migration-and-invasion-by-inactivating-the-ctnnb1-mediated-notch-signaling-pathway
#9
Bao-Long Pan, Ling Wu, Li Pan, Yu-Xi Yang, Hu-Huan Li, Yan-Juan Dai, Zi-Qian He, Ling Tan, You-Guang Huang, Zong-Wu Tong, Jun-Long Liao
Osteosarcoma (OS) is the most common histological form of primary bone cancer. It is most prevalent in teenagers and young adults. This study aims at exploring the regulatory effect of microRNA-340 (miR-340) on OS cell proliferation, invasion, migration and apoptosis via regulating the Notch signaling pathway by targeting CTNNB1. OS tissues belonging to 45 patients and normal femoral head tissues of 45 amputees were selected. Cells were allocated to different groups. In Situ hybridization was performed to determine the positive rate of miR-340 expression while immunohistochemistry was used to determine that of CTNNB1 and B-cell lymphoma 2 (Bcl-2)...
May 16, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29769249/spatial-distribution-and-function-of-t-follicular-regulatory-cells-in-human-lymph-nodes
#10
Ismail Sayin, Andrea J Radtke, Laura A Vella, Wenjie Jin, E John Wherry, Marcus Buggert, Michael R Betts, Ramin S Herati, Ronald N Germain, David H Canaday
T follicular regulatory (Tfr) cells are a population of CD4+ T cells that express regulatory T cell markers and have been shown to suppress humoral immunity. However, the precise mechanisms and location of Tfr-mediated suppression in the lymph node (LN) microenvironment are unknown. Using highly multiplexed quantitative imaging and functional assays, we examined the spatial distribution, suppressive function, and preferred interacting partners of Tfr cells in human mesenteric LNs. We find that the majority of Tfr cells express low levels of PD-1 and reside at the border between the T cell zone and B cell follicle, with very few found in the germinal centers (GCs)...
May 16, 2018: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29768444/ensemble-methods-for-stochastic-networks-with-special-reference-to-the-biological-clock-of-neurospora-crassa
#11
C Caranica, A Al-Omari, Z Deng, J Griffith, R Nilsen, L Mao, J Arnold, H-B Schüttler
A major challenge in systems biology is to infer the parameters of regulatory networks that operate in a noisy environment, such as in a single cell. In a stochastic regime it is hard to distinguish noise from the real signal and to infer the noise contribution to the dynamical behavior. When the genetic network displays oscillatory dynamics, it is even harder to infer the parameters that produce the oscillations. To address this issue we introduce a new estimation method built on a combination of stochastic simulations, mass action kinetics and ensemble network simulations in which we match the average periodogram and phase of the model to that of the data...
2018: PloS One
https://www.readbyqxmd.com/read/29766831/phagocytosis-and-motility-in-human-neutrophils-is-competent-but-compromised-by-pharmacological-inhibition-of-ezrin-phosphorylation
#12
Rhiannon E Roberts, Geetha L Elumalai, Maurice B Hallett
BACKGROUND AND OBJECTIVE: Ezrin links the cortical cytoskeleton to the plasma membrane and plays a role in regulating cell shape change. Recently NSC668394 has been shown to inhibit a key step for its activity, i.e. phosphorylation at threonine 567. In neutrophils, another key regulatory step is the Ca2+-mediated cleavage of ezrin by calpain. METHODS: In this paper we use NSC668394 as a pharmacological inhibitor to investigate the interplay between these two steps in regulating neutrophil shape change...
May 15, 2018: Current Molecular Pharmacology
https://www.readbyqxmd.com/read/29765931/decreased-breg-th17-ratio-improved-the-prognosis-of-patients-with-ulcerative-colitis
#13
Xue Bing, Liang Linlang, Chen Keyan
Objective: To investigate the effects of regulatory B (Breg) cells and T helper 17 (Th17) cells on pathogenesis of ulcerative colitis, explore the clinical significance of Breg/Th17 ratio on the prognosis of ulcerative colitis, and provide the theoretical basis for the targeted therapy, diagnosis, and prognosis of the disease. Methods: Peripheral blood and colonic mucosa were collected from patients with ulcerative colitis. Hematoxylin-eosin staining was used to observe the pathological changes of colonic mucosa...
2018: Canadian Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29765543/igg4-related-kidney-disease-the-effects-of-a-rituximab-based-immunosuppressive-therapy
#14
Giacomo Quattrocchio, Antonella Barreca, Andrea Demarchi, Laura Solfietti, Giulietta Beltrame, Roberta Fenoglio, Michela Ferro, Paola Mesiano, Stefano Murgia, Giulio Del Vecchio, Carlo Massara, Cristiana Rollino, Dario Roccatello
IgG4-related disease (IgG4-RD) is a recently recognized disorder, characterized by elevated serum IgG4 concentrations, dense tissue infiltration of IgG4-positive plasma cells and storiform fibrosis. Treatment is usually based on steroids, however, relapses and long-term adverse effects are frequent. We prospectively studied 5 consecutive patients with histologically-proven IgG4-RD and renal involvement, treated with an extended Rituximab protocol combined with steroids. Two doses of intravenous cyclophosphamide were added in 4 patients...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29764941/a-trypanosome-specific-protein-cooperates-with-the-cif1-protein-to-promote-cytokinesis-in-trypanosoma-brucei
#15
Yasuhiro Kurasawa, Huiqing Hu, Qing Zhou, Ziyin Li
Cytokinesis, the terminal step in cell division, in the protist human pathogen Trypanosoma brucei occurs along the longitudinal axis from the anterior tip of the new flagellum attachment zone (FAZ) towards the posterior cell tip. This process is regulated by a signaling cascade composed of the Polo-like kinase homolog TbPLK, the Aurora B kinase homolog TbAUK1, and the trypanosome-specific CIF1-CIF2 protein complex. However, the regulatory mechanism and the signaling pathway for this unusual mode of cytokinesis remain poorly understood...
May 15, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29764494/precision-medicine-becomes-reality-tumor-type-agnostic-therapy
#16
REVIEW
Li Yan, Wei Zhang
Precision medicine just witnessed two breakthroughs in oncology in 2017. Pembrolizumab (Keytruda), Merck's anti-programmed cell death-1 (PD-1) monoclonal antibody (mAb), received accelerated approval in May 2017 by the US Food and Drug Administration for the treatment of adult and pediatric patients with unresectable or metastatic solid tumors that have been identified as having microsatellite instability-high (MSI-H) or deficient DNA mismatch repair (dMMR). Shortly after, nivolumab (Opdivo), Bristol-Myers Squibb's anti-PD-1 mAb, gained an accelerated approval in August 2017 for adult and pediatric patients with MSI-H or dMMR metastatic colorectal cancer that has progressed after standard chemotherapy...
March 31, 2018: Cancer communications
https://www.readbyqxmd.com/read/29764418/engineering-xylose-metabolism-for-production-of-polyhydroxybutyrate-in-the-non-model-bacterium-burkholderia-sacchari
#17
Linda P Guamán, Carlos Barba-Ostria, Fuzhong Zhang, Edmar R Oliveira-Filho, José Gregório C Gomez, Luiziana F Silva
BACKGROUND: Despite its ability to grow and produce high-value molecules using renewable carbon sources, two main factors must be improved to use Burkholderia sacchari as a chassis for bioproduction at an industrial scale: first, the lack of molecular tools to engineer this organism and second, the inherently slow growth rate and poly-3-hydroxybutyrate [P(3HB)] production using xylose. In this work, we have addressed both factors. RESULTS: First, we adapted a set of BglBrick plasmids and showed tunable expression in B...
May 15, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29763903/central-precocious-puberty-caused-by-a-heterozygous-deletion-in-the-mkrn3-promoter-region
#18
Delanie Macedo, Monica M França, Luciana Montenegro, Marina Cunha-Silva, Danielle S Bessa, Ana Paula Abreu, Ursula B Kaiser, Berenice B Mendonca, Alexander A L Jorge, Vinicius N Brito, Ana C Latronico
<br>Context: Loss-of-function mutations in the coding region of MKRN3, a maternally imprinted gene at chromosome 15q11.2, are a common cause of familial central precocious puberty (CPP). Whether MKRN3 alterations in regulatory regions can cause CPP has not been explored to date. We aimed to investigate potential pathogenic variants in the promoter region of MKRN3 in patients with idiopathic CPP. Patients/ Methods: A cohort of 110 patients with idiopathic CPP was studied. Family history of precocious sexual development was present in 25%...
May 15, 2018: Neuroendocrinology
https://www.readbyqxmd.com/read/29763795/protective-effects-of-echinacoside-against-anoxia-reperfusion-injury-in-h9c2-cells-via-up-regulating-p-akt-and-slc8a3
#19
Min Chen, Xiaodong Wang, Bo Hu, Jian Zhou, Xin Wang, Wei Wei, Hua Zhou
Echinacoside is a natural ingredient with various pharmacological activities. In this study, we investigated the protective effects of echinacoside on cardiomyocytes (rat H9c2 cells) in an anoxia/reperfusion (A/R) model. Further, the regulatory function of sodium-calcium exchanger protein 3 (SLC8A3/NCX3) as well as the protein kinase B (AKT) signaling were studied. The present results indicated that echinacoside protected against A/R-induced apoptosis in a dose manner, which was characterized by a decrease in the apoptosis and caspase 3 protein levels in H9c2 cells...
May 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29763768/effects-on-estrogen-dependent-and-triple-negative-breast-cancer-cells-growth-of-ni-ii-zn-ii-and-cd-ii-complexes-with-the-schiff-base-derived-from-pyridine-2-carboxaldehyde-and-5-6-diamino-1-3-dimethyluracil-explored-through-the-renin-angiotensin-system-ras
#20
Nuria A Illán-Cabeza, Sonia B Jiménez-Pulido, Francisco Hueso-Ureña, María J Ramírez-Expósito, Purificación Sánchez-Sánchez, José M Martínez-Martos, Miguel N Moreno-Carretero
A series of Ni(II), Zn(II) and Cd(II) complexes with the Schiff base derived from the condensation 1:1 from pyridine-2-carboxaldehyde and 5,6-diamino-1,3-dimethyluracil (6-amino-1,3-dimethyl-5-[(pyridin-2-ylmethylidene)-amino]pyrimidine-2,4(1H,3H)-dione, DAAUPic) were synthesized and subsequently characterized by means of elemental analysis, FT-IR, NMR and nine of them by X-ray diffraction. Except the [Zn(μ-O,O'-AcO)(N5 ,N6 ,N1F -DAAUPicH-1 )]2 and [Cd(O,O'-NO3 )(μ-O4 ,(N5 ,N6 ,N1F )-DAAUPicH-1 )(H2 O)]2 ·2H2 O dimers and the [Cd(μ-S,N-SCN)(N5 ,N6 ,N1F -DAAUPicH-1 )]n chain-like polymer, all of them display monomeric molecular structures...
May 9, 2018: Journal of Inorganic Biochemistry
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