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Regenerative medicine

Azam Sargazi, Fereshteh Shiri, Sheida Keikha, Mostafa Heidari Majd
Hyaluronic acid (HA) is increasingly investigated for biomedical applications such as regenerative medicine, aesthetic medicine, and drug delivery. Accordingly, conjugation of HA to PEGylated MNPs could increase the active targeting ability of nano-drug carriers toward CD44 receptors and be useful in a clinical setting such as drug delivery. So, we chemically conjugated mitoxantrone (MTX) to HA-PEGylated MNPs to use concurrent advantages such as prolong the circulation time, decrease the side effects and delivery toward special tumor cells...
July 11, 2018: Colloids and Surfaces. B, Biointerfaces
Xiaoniao Chen, Lingling Wu, Zongjin Li, Ying Dong, Xuetao Pei, Yifei Huang, Liqiang Wang
Purpose: A shortage of human corneal endothelial cells (HCEC) for transplant and current methods of differentiation induction require chemical compounds, which might cast further influences after differentiation induction. Therefore, we developed a simple and straightforward approach to endothelial cell differentiation from human embryonic stem cells (hESC). Methods: HESC are used to differentiate into HCEC by employing a two-stage method, which involves the application of two different types of conditioned culture medium, human corneal stromal cell-conditioned medium (HCSC-CM) and lens epithelial cell (LEC) plus HCSC-CM (LEC-CM+HCEC-CM)...
June 1, 2018: Investigative Ophthalmology & Visual Science
Giuseppe Criscenti, Carmelo De Maria, Alessia Longoni, Clemens A van Blitterswijk, Hugo A M Fernandes, Giovanni Vozzi, Lorenzo Moroni
The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Scaffolds functionalized using different proteins and growth factors (GFs) arranged onto the surface were designed, fabricated and validated with different polyesters, demonstrating the versatility of the developed approach...
July 19, 2018: Biofabrication
Yulei Wei, Ganesh N Pandian, Zutao Yu, Tingting Zou, Yue Li, Jayant Darokar, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Synthetic DNA-binding inhibitors capable of gaining precise control over neurogenesis factors could obviate the current clinical barriers associated with the use of small molecules in regenerative medicine. Here, we report the design and bioefficacy of the synthetic ligand PIP-RBPJ-1, which caused promoter-specific suppression of neurogenesis-associated HES1 and its downstream genes. Furthermore, PIP-RBPJ-1 alone altered the neural-system-associated Notch-signaling factors and remarkably induced neurogenesis with an efficiency that was comparable to that of a conventional approach...
March 31, 2018: ACS Omega
Yinshan Bai, Cui Zhu, Meiying Feng, Hengxi Wei, Li Li, Xiuchun Tian, Zhihong Zhao, Shanshan Liu, Ningfang Ma, Xianwei Zhang, Ruyi Shi, Chao Fu, Zhenfang Wu, Shouquan Zhang
BACKGROUND: Male germline stem cells (mGSCs) offer great promise in regenerative medicine and animal breeding due to their capacity to maintain self-renewal and to transmit genetic information to the next generation following spermatogenesis. Human testis-derived embryonic stem cell-like cells have been shown to possess potential of mesenchymal progenitors, but there remains confusion about the characteristics and origin of porcine testis-derived stem cells. METHODS: Porcine testis-derived stem cells were obtained from primary testicular cultures of 5-day old piglets, and selectively expanded using culture conditions for long-term culture and induction differentiation...
July 18, 2018: Stem Cell Research & Therapy
Vania Vieira, Ana Lima, Menno de Jong, David K Smith
This paper reports the ability of synthetically-simple, commercially-viable sugar-derived 1,3:2,4-dibenzylidenesorbitol-4',4"-diacylhydrazide (DBS-CONHNH2) to support cell growth. Simple mixing and orthogonal self-sorting can formulate heparin, agarose, and heparin-binding micelles into these gels - easily incorporating additional function. Interestingly, the components used in the gel formulation, direct the ability of cells to grow, meaning the chemical programming of these multi-component gels is directly translated to the biological systems in contact with them...
July 18, 2018: Chemistry: a European Journal
Devang K Thakor, Lei Wang, Darcy Benedict, Serdar Kabatas, Ross D Zafonte, Yang D Teng
Human mesenchymal stromal stem cells (hMSCs) hold regenerative medicine potential due to their availability, in vitro expansion readiness, and autologous feasibility. For neural repair, hMSCs show translational value in research on stroke, spinal cord injury (SCI), and traumatic brain injury. It is pivotal to establish multimodal in vitro systems to investigate molecular mechanisms underlying neural actions of hMSCs. Here, we describe a platform protocol on how to set up organotypic co-cultures of hMSCs (alone or polymer-scaffolded) with explanted adult rat dorsal root ganglia (DRGs) to determine neural injury and recovery events for designing implants to counteract neurotrauma sequelae...
July 18, 2018: Current Protocols in Stem Cell Biology
Zifei Zhou, Xifeng Liu, Wei Wu, Sungjo Park, A Lee Miller Ii, Andre Terzic, Lichun Lu
Biomimetic biomaterials require good biocompatibility and bioactivity to serve as appropriate scaffolds for tissue engineering applications. Recent developments demonstrated that the unique properties of carbon nanotubes (CNTs) can enhance neural cell growth and axon organization. We previously developed a promising nerve conduit manufactured from biodegradable polycaprolactone fumarate (PCLF) for use in peripheral nerve regeneration applications. In the present study, we fabricated conductive PCLF-CNT scaffolds using ultraviolet (UV) induced photocrosslinking...
July 18, 2018: Biomaterials Science
Guangcun Chen, Yejun Zhang, Chunyan Li, Dehua Huang, Qianwu Wang, Qiangbin Wang
Stem cell-based regenerative medicine has attracted tremendous attention for its great potential to treat numerous incurable diseases. Tracking and understanding the fate and regenerative capabilities of transplanted stem cells is vital for improving the safety and therapeutic efficacy of stem cell-based therapy, therefore accelerating the clinical application of stem cells. Fluorescent nanoparticles (NPs) have been widely used for in vivo tracking of the transplanted stem cells. Among these fluorescent NPs, near-infrared (NIR) NPs have greatly improved the sensitivity, tissue penetration depth, spatial and temporal resolutions of the fluorescence imaging-based stem cell tracking technologies due to the reduced absorption, scattering, and autofluorescence of NIR fluorescence in tissues...
July 17, 2018: Advanced Healthcare Materials
A Barco, E Ingham, J Fisher, H Fermor, R P W Davies
Peptide-based hydrogels are of interest for their potential use in regenerative medicine. Combining these hydrogels with materials that may enhance their physical and biological properties, such as glycosaminoglycans, has the potential to extend their range of biomedical applications, for example in the repair of early cartilage degeneration. The aim of this study was to combine three self-assembling peptides (P11 -4, P11 -8, and P11 -12) with chondroitin sulphate at two molar ratios of 1:16 and 1:64 in 130 and 230 mM Na+ salt concentrations...
July 18, 2018: Journal of Peptide Science: An Official Publication of the European Peptide Society
Florian Friedmacher, Mikko P Pakarinen, Risto J Rintala
Despite a growing interest to clinicians and scientists, there is no comprehensive study that examines the global research activity on congenital diaphragmatic hernia (CDH). A search strategy for the Web of Science™ database was designed to identify scientific CDH publications. Research output of countries, institutions, individual authors, and collaborative networks was analyzed. Semi-qualitative research measures including citation rate and h-index were assessed. Choropleth mapping and network diagrams were employed to visualize results...
July 17, 2018: Pediatric Surgery International
Eoghan M Cunnane, Justin S Weinbaum, Fergal J O'Brien, David A Vorp
Vascular tissue engineering is an area of regenerative medicine that attempts to create functional replacement tissue for defective segments of the vascular network. One approach to vascular tissue engineering utilizes seeding of biodegradable tubular scaffolds with stem (and/or progenitor) cells wherein the seeded cells initiate scaffold remodeling and prevent thrombosis through paracrine signaling to endogenous cells. Stem cells have received an abundance of attention in recent literature regarding the mechanism of their paracrine therapeutic effect...
2018: Frontiers in Cardiovascular Medicine
Abbas Beh-Pajooh, Tobias Cantz
Research on stem cells is one of the fastest growing areas of regenerative medicine that paves the way for a comprehensive solution to cell therapy. Today, stem cells are precious assets for generating different types of cells derived from either natural embryonic stem (ES) cells or induced pluripotent stem (iPS) cells. The iPS technology can revolutionize the future of clinics by offering personalized medicine, which will provide the future treatment for curing untreatable diseases. Although iPS cell therapy is now at its infancy, promising research has motivated scientists to pursue this therapeutic approach...
2018: Journal of Stem Cells & Regenerative Medicine
Geoffrey Banda, Joyce Tait, James Mittra
PURPOSE: This article focuses on 10 case studies of companies/organizations that are part of the current innovation ecosystem of regenerative medicine (RM) in the United Kingdom. It analyzes the actors, linkages, and influences that will determine the future shape of the RM industry sector and its capacity to live up to its initial expectations. METHODS: Using the case study approach, purposive sampling was used to get 18 interview respondents from 10 RM companies/organizations in the United Kingdom...
July 14, 2018: Clinical Therapeutics
Zhaoyu Ba, Zhaoxiong Chen, Yufeng Huang, Du Feng, Qinghui Zhao, Jianguang Zhu, Desheng Wu
Introduction: It is predicted that with increased life expectancy in the whole world, there will be a greater demand for synthetic biomedical materials to repair or regenerate lost, injured or diseased tissues. Natural polymers, as biomedical materials, have been widely applied in the field of regenerative medicine. Materials and methods: By incorporation of nanoporous diopside bioglass (nDPB) into glia-din (GL) matrix, macro-nanoporous scaffolds of nDPB/GL composites (DGC) were fabricated by method of solution compressing and particles leaching...
2018: International Journal of Nanomedicine
Nela Pilbauerová, Jakub Suchánek
Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies...
2018: Acta Medica (Hradec Králové)
Dhanak Gupta, Kazi M Zakir Hossain, Ifty Ahmed, Virginie Sottile, David M Grant
The chemical formulation of phosphate based glasses (PBGs) can be tailored to fit particular end applications such as bone tissue engineering. While most reports to date have evaluated the effect of PBG chemical formulation on bone cells, this study specifically explored the manufacturing process, the changes in physical and chemical properties of PBG particles after flame spheroidisation, and subsequent effects on human mesenchymal stem cells (hMSCs), a prime cell type for regenerative medicine applications...
July 16, 2018: ACS Applied Materials & Interfaces
Prathap Jayaram, Uzoh Ikepeama, Joshua B Rothenberg, Gerard A Malanga
Regenerative medicine in the context of musculoskeletal injury is a broad term that offers potential therapeutic solutions to restore or repair damaged tissue. The current focus in recent literature and clinical practice has been on cell based therapy. In particular, much attention has been centered on autologous bone marrow concentrate (BMAC) and adipose derived (AD) mesenchymal stem cells (MSCs) for cartilage and tendon disorders. This article provides an overview of MSC derived therapy and offers a comprehensive review of Adipose and Bone Marrow derived MSC therapy in primary knee osteoarthritis OA...
July 13, 2018: PM & R: the Journal of Injury, Function, and Rehabilitation
Joshua G Hunsberger, Thomas Shupe, Anthony Atala
Regenerative medicine is poised to become a significant industry within the medical field. As such, the development of strategies and technologies for standardized and automated regenerative medicine clinical manufacturing has become a priority. An industry-driven roadmap toward industrial scale clinical manufacturing was developed over a 3-year period by a consortium of companies with significant investment in the field of regenerative medicine. Additionally, this same group identified critical roadblocks that stand in the way of advanced, large-scale regenerative medicine clinical manufacturing...
July 15, 2018: Stem Cells Translational Medicine
Yuji Haraguchi, Katsuhisa Matsuura, Yuki Kagawa, Akiyuki Hasegawa, Hirotsugu Kubo, Tatsuya Shimizu
This study reports a rapid fabrication system of a morphologically and functionally communicative 3-D cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37°C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 µm-thickness were created at 1.5 h [centrifugation (80 x g, 37°C) for 30 min and tissue harvest for 1 h]...
July 16, 2018: Biotechnology Progress
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