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Regenerative medicine

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https://www.readbyqxmd.com/read/29356242/preferable-in-vitro-condition-for-maintaining-faithful-dna-methylation-imprinting-in-mouse-embryonic-stem-cells
#1
Jiyoung Lee, Ayumi Matsuzawa, Hirosuke Shiura, Akito Sutani, Fumitoshi Ishino
Epigenetic properties of cultured embryonic stem cells (ESCs), including DNA methylation imprinting, are important because they affect the developmental potential. Here, we tested a variety of culture media, including knockout serum replacement (KSR) and fetal bovine serum (FBS) with or without inhibitors of Gsk3β and Mek1/2 (2i) at various time points. In addition to the previously known passage-dependent global changes, unexpected dynamic DNA methylation changes occurred in both maternal and paternal differentially methylated regions: under the widely used condition of KSR with 2i, a highly hypomethylated state occurred at early passages (P1-7) as well as P10, but DNA methylation increased over further passages in most conditions, except under KSR with 2i at P25...
January 22, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29355456/icraiin-improves-fanconi-anemia-hematopoietic-stem-cell-function-through-sirt6-mediated-nf-kappa-b-inhibition
#2
Yibo Li, Xue Li, Allison Cole, Sarah McLaughlin, Wei Du
Icraiin (ICA) is a flavonoid glucoside derived from the Epimedium plant genus, which has potent regenerative properties and is used in western medicine to treat impotence. Recently, ICA has generated great interest in improving hepatic stellate cell function and cardiac rejuvenation. However, how this natural component functions in hematopoiesis remains unexplored. Here we have examined the role of ICA on hematopoietic stem cells (HSCs) using the cancer-prone disease model of Fanconi anemia (FA), an inherited bone marrow failure syndrome with extremely high risk of leukemic predisposition...
January 22, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29355413/treatment-of-carious-lesions-using-self-assembling-peptides
#3
M Alkilzy, R M Santamaria, J Schmoeckel, C H Splieth
Modern approaches in caries treatment involve lesion management without tissue removal. Regenerative medicine focuses on replacing damaged tissues with biologically similar tissues. This article discusses the scientific evidence and clinical results for self-assembling peptides in modern caries management. The biomimetic remineralization promoted by self-assembling peptide P11-4 has been proven in vitro as an effective therapy for initial caries. P11-4 was rationally designed to promote formation of hydroxyapatite on its surface...
February 2018: Advances in Dental Research
https://www.readbyqxmd.com/read/29353456/in-vivo-transient-and-partial-cell-reprogramming-to-pluripotency-as-a-therapeutic-tool-for-neurodegenerative-diseases
#4
REVIEW
S Tamanini, G P Comi, S Corti
In theory, human diseases in which a specific cell type degenerates, such as neurodegenerative diseases, can be therapeutically addressed by replacement of the lost cells. The classical strategy for cell replacement is exogenous cell transplantation, but now, cell replacement can also be achieved with in situ reprogramming. Indeed, many of these disorders are age-dependent, and "rejuvenating" strategies based on cell epigenetic modifications are a possible approach to counteract disease progression. In this context, transient and/or partial reprogramming of adult somatic cells towards pluripotency can be a promising tool for neuroregeneration...
January 20, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29352448/mesenchymal-stem-cell-deformability-and-implications-for-microvascular-sequestration
#5
Herbert H Lipowsky, Daniel T Bowers, Britany L Banik, Justin L Brown
Mesenchymal stem cells (MSCs) have received considerable attention in regenerative medicine, particularly in light of prospects for targeted delivery by intra-arterial injection. However, little is known about the mechanics of MSC sequestration in the microvasculature and the yield pressure (PY), above which MSCs will pass through microvessels of a given diameter. The objectives of the current study were to delineate the dependency of PY on cell size and the heterogeneity of cell mechanical properties and diameters (DCELL) of cultured MSCs...
January 19, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29352188/efficient-scalable-production-of-therapeutic-microvesicles-derived-from-human-mesenchymal-stem-cells
#6
Jae Min Cha, Eun Kyoung Shin, Ji Hee Sung, Gyeong Joon Moon, Eun Hee Kim, Yeon Hee Cho, Hyung Dal Park, Hojae Bae, Jinseok Kim, Oh Young Bang
Microvesicles (MVs) released by cells are involved in a multitude of physiological events as important mediators of intercellular communication. MVs derived from mesenchymal stem cells (MSCs) contain various paracrine factors from the cells that primarily contribute to their therapeutic efficacy observed in numerous clinical trials. As nano-sized and bi-lipid layered vesicles retaining therapeutic potency equivalent to that of MSCs, MSC-derived MVs have been in focus as ideal medicinal candidates for regenerative medicine, and are preferred over MSC infusion therapy with their improved safety profiles...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352121/efficient-differentiation-of-human-pluripotent-stem-cells-into-skeletal-muscle-cells-by-combining-rna-based-myod1-expression-and-pou5f1-silencing
#7
Tomohiko Akiyama, Saeko Sato, Nana Chikazawa-Nohtomi, Atsumi Soma, Hiromi Kimura, Shunichi Wakabayashi, Shigeru B H Ko, Minoru S H Ko
Direct generation of skeletal muscle cells from human pluripotent stem cells (hPSCs) would be beneficial for drug testing, drug discovery, and disease modelling in vitro. Here we show a rapid and robust method to induce myogenic differentiation of hPSCs by introducing mRNA encoding MYOD1 together with siRNA-mediated knockdown of POU5F1 (also known as OCT4 or OCT3/4). This integration-free approach generates functional skeletal myotubes with sarcomere-like structure and a fusion capacity in several days. The POU5F1 silencing facilitates MYOD1 recruitment to the target promoters, which results in the significant activation of myogenic genes in hPSCs...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29349512/photobiomodulation-induces-in-vitro-re-epithelialization-via-nitric-oxide-production
#8
Manuela Rizzi, Mario Migliario, Stelvio Tonello, Vincenzo Rocchetti, Filippo Renò
Photobiomodulation is a widely used tool in regenerative medicine thanks to its ability to modulate a plethora of physiological responses. Wound re-epithelialization is strictly regulated by locally produced chemical mediators, such as nitric oxide (NO), a highly reactive free radical generated by the nitric oxide synthase (NOS) enzymatic family. In this study, it has been hypothesized that a 980-nm low-level laser stimulation could increase NO production in human keratinocytes and that such event might be directly related to the re-epithelialization process...
January 18, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29348578/a-matter-of-life-and-death-stem-cell-survival-in-tissue-regeneration-and-tumour-formation
#9
REVIEW
Despina Soteriou, Yaron Fuchs
In recent years, great strides have been made in our understanding of how stem cells (SCs) govern tissue homeostasis and regeneration. The inherent longevity of SCs raises the possibility that the unique protective mechanisms in these cells might also be involved in tumorigenesis. In this Opinion article, we discuss how SCs are protected throughout their lifespan, focusing on quiescent behaviour, DNA damage response and programmed cell death. We briefly examine the roles of adult SCs and progenitors in tissue repair and tumorigenesis and explore how signals released from dying or dormant cells influence the function of healthy or aberrant SCs...
January 19, 2018: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/29348248/texas-heart-institute-international-symposium-on-cardiovascular-regenerative-medicine
#10
Emerson C Perin, Doris A Taylor
No abstract text is available yet for this article.
January 19, 2018: Circulation Research
https://www.readbyqxmd.com/read/29348246/global-overview-of-the-transnational-alliance-for-regenerative-therapies-in-cardiovascular-syndromes-tactics-recommendations-a-comprehensive-series-of-challenges-and-priorities-of-cardiovascular-regenerative-medicine
#11
EDITORIAL
Francisco Fernández-Avilés, Ricardo Sanz-Ruiz, Andreu M Climent, Lina Badimon, Roberto Bolli, Dominique Charron, Valentin Fuster, Stefan Janssens, Jens Kastrup, Hyo-Soo Kim, Thomas F Lüscher, John F Martin, Philippe Menasché, Fausto J Pinto, Robert D Simari, Gregg W Stone, Andre Terzic, James T Willerson, Joseph C Wu
No abstract text is available yet for this article.
January 19, 2018: Circulation Research
https://www.readbyqxmd.com/read/29346426/proliferation-and-survival-of-human-amniotic-epithelial-cells-during-their-hepatic-differentiation
#12
Julieta L Maymó, Rodrigo Riedel, Antonio Pérez-Pérez, Marta Magatti, Bernardo Maskin, José Luis Dueñas, Ornella Parolini, Víctor Sánchez-Margalet, Cecilia L Varone
Stem cells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles...
2018: PloS One
https://www.readbyqxmd.com/read/29345715/tendon-function-related-structure-simple-healing-process-and-mysterious-ageing
#13
Jan Zabrzyński, Łukasz Łapaj, Łukasz Paczesny, Agnieszka Zabrzyńska, Dariusz Grzanka
Tendons are connective tissue structures of paramount importance to human ability of locomotion. The understanding of their physiology and pathology is gaining importance as advances in regenerative medicine are being made today. So far, very few studies were conducted to extend the knowledge about pathology, healing response and management of tendon lesions. In this paper we summarize actual knowledge on structure, process of healing and ageing of the tendons. The structure of tendon is optimized for the best performance of the tissue...
January 18, 2018: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29345324/autocrine-signals-increase-ovine-mesenchymal-stem-cells-migration-through-aquaporin-1-and-cxcr4-overexpression
#14
Alessandra Pelagalli, Anna Nardelli, Enrico Lucarelli, Antonella Zannetti, Arturo Brunetti
Sheep is a relevant large animal model that is frequently used to test innovative tissue engineering (TE) approaches especially for bone reconstruction. Mesenchymal stem cells (MSCs) are used in TE applications because they represent key component of adult tissue repair. Importantly, MSCs from different species show similar characteristics, which facilitated their application in translational studies using animal models. Nowadays, many researches are focusing on the use of ovine mesenchymal stem cells (oMSCs) in orthopedic preclinical settings for regenerative medicine purposes...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29344013/isbt-128-standard-for-coding-medical-products-of-human-origin
#15
REVIEW
Paul Ashford, Matthew Delgado
Background: ISBT 128 is an international standard for the terminology, coding, labeling, and identification of medical products of human origin (MPHO). Full implementation of ISBT 128 improves traceability, transparency, vigilance and surveillance, and interoperability. Methods: ICCBBA maintains the ISBT 128 standard through the activities of a network of expert volunteers, including representatives from professional scientific societies, governments and users, to standardize and maintain MPHO identification...
November 2017: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/29343270/isolation-and-characterization-of-olfactory-ecto-mesenchymal-stem-cells-from-eight-mammalian-genera
#16
Antoine D Veron, Cécile Bienboire-Frosini, François Feron, Elisa Codecasa, Arnaud Deveze, Dany Royer, Paul Watelet, Pietro Asproni, Kevin Sadelli, Camille Chabaud, Jean-Claude Stamegna, Joël Fagot, Michel Khrestchatisky, Alessandro Cozzi, François S Roman, Patrick Pageat, Manuel Mengoli, Stéphane D Girard
BACKGROUND: Stem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, "olfactory ecto-mesenchymal stem cells" (OE-MSCs) have been described as a promising candidate to treat a variety of damaged tissues. Easily accessible in the nasal cavity of most mammals, these cells are highly suitable for autologous cell-based therapies and do not face issues associated with other stem cells...
January 17, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29341933/the-effects-of-platelet-lysate-patches-on-the-activity-of-tendon-derived-cells
#17
Raquel Costa-Almeida, Albina R Franco, Tamagno Pesqueira, Mariana B Oliveira, Pedro S Babo, Isabel B Leonor, João F Mano, Rui L Reis, Manuela E Gomes
Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB...
January 13, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29341813/regenerative-medicine-a-vehicle-to-infuse-laboratory-bench-modules-into-an-exercise-physiology-curriculum
#18
Jason M Brown, Breonte S Guy, Dawn X Henderson, C Edward Ebert, Jill Harp, Chad D Markert
Regenerative medicine is a novel discipline that both excites undergraduates and may be used as a vehicle to expose students to scientific concepts and opportunities. The goal of this article is to describe the implementation of a National Science Foundation-funded Targeted Infusion Project in which underrepresented minority undergraduates are exposed to laboratory-bench skills and summer research opportunities that they may not have encountered otherwise. A 3-wk infusion of laboratory-bench and data presentation skills, in the context of a regenerative medicine/bioengineering project, aimed to engage students and expose them to opportunities as summer researchers and teaching assistants...
March 1, 2018: Advances in Physiology Education
https://www.readbyqxmd.com/read/29341292/amniotic-fluid-stem-cell-exosomes-therapeutic-perspective
#19
Francesca Beretti, Manuela Zavatti, Francesca Casciaro, Giuseppina Comitini, Fabrizia Franchi, Veronica Barbieri, Giovanni B La Sala, Tullia Maraldi
It is widely accepted that the therapeutic potential of stem cells can be largely mediated by paracrine factors, also included into exosomes. Thus, stem cell-derived exosomes represent a major therapeutic option in regenerative medicine avoiding, if compared to stem cells graft, abnormal differentiation and tumor formation. Exosomes derived from mesenchymal stem cells (MSC) induce damaged tissue repair, and can also exert immunomodulatory effects on the differentiation, activation and function of different lymphocytes...
January 16, 2018: BioFactors
https://www.readbyqxmd.com/read/29340876/biomaterials-and-regenerative-medicine-in-urology
#20
N F Davis, E M Cunnane, M R Quinlan, J J Mulvihill, N Lawrentschuk, D M Bolton, M T Walsh
Autologous gastrointestinal tissue is the gold standard biomaterial for urinary tract reconstruction despite its long-term neuromechanical and metabolic complications. Regenerative biomaterials have been proposed as alternatives; however many are limited by a poor host derived regenerative response and deficient supportive elements for effective tissue regeneration in vivo. Urological biomaterials are sub-classified into xenogenic extracellular matrices (ECMs) or synthetic polymers. ECMs are decellularised, biocompatible, biodegradable biomaterials derived from animal organs...
January 17, 2018: Advances in Experimental Medicine and Biology
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