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https://www.readbyqxmd.com/read/29054092/protein-mimicking-nanoparticles-for-the-reproduction-of-transient-protein-receptor-interactions
#1
Jonghwan Lee
One of the major concerns in target identification has been the need for new methods to detect target molecules in the native cellular environment. In conventional target identification, affinity-based pull down has been conducted using cell lysates. However, interactions in cell lysate do not reflect real endogenous interactions in living cells, and can produce false-positive or false-negative results. This study aimed to develop a new method of target protein identification in living cells. Targeting probes were conjugated onto magnetic nanoparticles (MNPs)...
October 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29054080/efficient-degradation-of-selected-polluting-dyes-using-the-tetrahydroxoargentate-ion-ag-oh-4-in-alkaline-media
#2
Ileana R Zamora-Garcia, Alejandro Alatorre-Ordaz, Jorge G Ibanez, Julio C Torres-Elguera, Kazimierz Wrobel, Silvia Gutierrez-Granados
The use of soluble and highly oxidizing Ag(III) in the form of the tetrahydroxoargentate ion Ag(OH)4(-) is reported for the oxidation of surrogate organic recalcitrant dyes (i.e., rhodamine 6G (Rh6G) and fluorescein (Fl)). The possible use of Ag(OH)4(-) for the treatment of these and other refractory compounds is assessed. Such dyes were selected due to their common occurrence, stability, refractory nature, the relatively high toxicity of Rh6G, and their structural similarity to Fl. Several reaction intermediates/products were identified...
October 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/29054025/antibacterial-activity-against-cariogenic-bacteria-and-cytotoxic-and-genotoxic-potential-of-anacardium-occidentale-l-and-anadenanthera-macrocarpa-benth-brenan-extracts
#3
Josenildo Segundo Chaves de Araújo, Aline Rogéria Freire de Castilho, Andressa Brito Lira, Andréia Vieira Pereira, Tatiane Kelly Barbosa de Azevêdo, Edja Maria de Melo de Brito Costa, Maria do Socorro Vieira Pereira, Hilzeth Freire Luna Pessoa, Jozinete Vieira Pereira
OBJECTIVES: The present study aimed to assess the antibacterial activity against bacteria with cariogenic relevance, toxic and genotoxic potential of the plants Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Bernam. DESIGN: Using a microdilution technique, the extracts were submitted to minimum inhibitory concentration (MIC) testing against Streptococcus mitis (ATCC 903), Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557), Streptococcus salivarius (ATCC 7073), Streptococcus sanguinis (ATCC 15300) and Streptococcus sobrinus (ATCC 27609)...
October 14, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29053942/do-elite-breath-hold-divers-suffer-from-mild-short-term-memory-impairments
#4
François Billaut, Patrice Gueit, Sylvane Faure, Guillaume Costalat, Frédéric Lemaitre
Repeated apneas are associated with severe hypoxemia that may ultimately lead to loss of consciousness in some breath-hold divers. Despite increasing number of practitioners, the relationship between apnea-induced hypoxia and neurocognitive functions is still poorly understood in the sport of free diving. To shed light onto this phenomenon, we examined the impact of long-term breath-hold diving training on attentional processing, short-term memory, and long-term mnesic and executive functions. Thirty-six men matched for age, height and weight were separated into three groups [12 elite breath-hold divers (EBHD, mean static apnea best time 371 sec, 105 months mean apnea experience); 12 novice breath-hold divers (NBHD, mean best time 243 sec, 8...
October 20, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29053937/studies-with-herpes-zoster-vaccines-in-immune-compromised-patients
#5
Myron J Levin, Eddy Bresnitz, Zoran Popmihajlov, Adriana Weinberg, Kai-Li Liaw, English Willis, Jeffrey R Curtis
INTRODUCTION: The active component of the herpes zoster vaccine (ZVL), recommended for people ≥50 years of age, is a live attenuated varicella-zoster virus. ZVL is contraindicated for immune compromised individuals, with limited regard to the degree of immunosuppression. Areas covered: This review evaluates phase I and II, and observational studies for ZVL and published reports of the off-label use of ZVL, for conditions and therapies for which investigators considered the risk-benefit for using ZVL to be favorable...
October 20, 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/29053900/hair-coat-properties-of-donkeys-mules-and-horses-in-a-temperate-climate
#6
B Osthaus, L Proops, S Long, N Bell, K Hayday, F Burden
BACKGROUND: There are clear differences between donkeys and horses in their evolutionary history, physiology, behaviour and husbandry needs. Donkeys are often kept in climates they are not adapted to and as such may suffer impaired welfare unless protection from the elements is provided. OBJECTIVES: To compare some of the hair coat properties of donkeys, mules and horses living outside, throughout the year, in the temperate climate of the UK. STUDY DESIGN: Longitudinal study...
October 20, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29053827/comparative-evaluation-of-texture-wheat-ingredients-and-soy-proteins-in-the-quality-and-acceptability-of-emulsified-chicken-nuggets
#7
M Yeater, G Casco, R K Miller, C Z Alvarado
Chicken nuggets are commonly made with varying levels of textured vegetable proteins, such as soy and wheat, for their ability to bind water and their meat like conformation. This project compared textured wheat proteins and soy proteins at 10%, 20%, 30%, and 40% in emulsified chicken nuggets. A total of 3,024 chicken nuggets were evaluated in replications for batter breader pickup (%), par fry yield (%), cook loss (%), L*, a*, b* color value, texture profile analysis, and sensory analysis. The analysis was conducted for all 4 concentrations of wheat and soy treatments then compared to each other and an all white meat chicken nugget control...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053815/insulin-immuno-neutralization-decreases-food-intake-in-chickens-without-altering-hypothalamic-transcripts-involved-in-food-intake-and-metabolism
#8
M Proszkowiec-Weglarz, J Dupont, N Rideau, C Gespach, J Simon, T E Porter
In mammals, insulin regulates blood glucose levels and plays a key regulatory role in appetite via the hypothalamus. In contrast, chickens are characterized by atypical glucose homeostasis, with relatively high blood glucose levels, reduced glucose sensitivity of pancreatic beta cells, and large resistance to exogenous insulin. The aim of the present study was to investigate in chickens the effects of 5 h fasting and 5 h insulin immuno-neutralization on hypothalamic mRNA levels of 23 genes associated with food intake, energy balance, and glucose metabolism...
September 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053771/the-cerebellum-in-alzheimer-s-disease-evaluating-its-role-in-cognitive-decline
#9
Heidi I L Jacobs, David A Hopkins, Helen C Mayrhofer, Emiliano Bruner, Fred W van Leeuwen, Wijnand Raaijmakers, Jeremy D Schmahmann
The cerebellum has long been regarded as essential only for the coordination of voluntary motor activity and motor learning. Anatomical, clinical and neuroimaging studies have led to a paradigm shift in the understanding of the cerebellar role in nervous system function, demonstrating that the cerebellum appears integral also to the modulation of cognition and emotion. The search to understand the cerebellar contribution to cognitive processing has increased interest in exploring the role of the cerebellum in neurodegenerative and neuropsychiatric disorders...
July 28, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29053754/coming-of-age-do-female-harbour-porpoises-phocoena-phocoena-from-the-north-sea-and-baltic-sea-have-sufficient-time-to-reproduce-in-a-human-influenced-environment
#10
Tina Kesselring, Sacha Viquerat, Ralph Brehm, Ursula Siebert
The harbour porpoise is the only cetacean species native to German waters. Since human pressures are suggested to shorten their reproductive lifespan, basic knowledge on reproduction is strongly required. One parameter is the onset of sexual maturity in female harbour porpoises. Therefore, we investigated the first signs of sexual maturity for a period of almost two decades (1990-2016). Ovaries from 111 female harbour porpoises from the German North Sea and Baltic Sea were examined for the presence and morphological structure of follicles, corpora lutea and corpora albicantia...
2017: PloS One
https://www.readbyqxmd.com/read/29053751/bacterial-community-analysis-of-anoxic-aeration-a-o-system-in-a-combined-process-for-gibberellin-wastewater-treatment
#11
Erming Ouyang, Yao Lu, Jiating Ouyang, Lele Wang, Xiaohui Wang
Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment...
2017: PloS One
https://www.readbyqxmd.com/read/29053737/screening-for-hiv-hepatitis-b-and-syphilis-on-dried-blood-spots-a-promising-method-to-better-reach-hidden-high-risk-populations-with-self-collected-sampling
#12
Inge H M van Loo, Nicole H T M Dukers-Muijrers, Rosalie Heuts, Marianne A B van der Sande, Christian J P A Hoebe
INTRODUCTION: Many people at high risk for sexually transmitted infections (STIs), e.g., men who have sex with men (MSM), are not optimally reached by current sexual health care systems with testing. To facilitate testing by home-based sampling or sampling in outreach setting we evaluated dried blood spots (DBS), a method for self-collected blood sampling for serological screening of HIV, hepatitis B (HBV) and syphilis. The aims of this study were to assess the acceptability and feasibility of self-collected DBS and to compare the test results for screening of HIV, HBV and syphilis from DBS with blood drawn by venous puncture...
2017: PloS One
https://www.readbyqxmd.com/read/29053712/reproductive-desire-among-women-living-with-hiv-aids-in-central-brazil-prevalence-and-associated-factors
#13
Marise Ramos de Souza, Waldemar Naves do Amaral, Rafael Alves Guimarães, Giovanni Rezza, Sandra Maria Brunini
BACKGROUND: The purpose of this study was to estimate the prevalence and examine the factors associated with reproductive desire among women living with HIV/AIDS (WLWHA) in Central Brazil. METHODS: A cross-sectional study involving 274 WLWHA, aged 18 to 49 years, was conducted with the support of treatment services and non-governmental organizations that assist people living with HIV/AIDS. Data regarding sociodemographic characteristics, substance use, sexual behavior, and reproductive variables were collected through interviews...
2017: PloS One
https://www.readbyqxmd.com/read/29053706/solea-senegalensis-sperm-cryopreservation-new-insights-on-sperm-quality
#14
Marta F Riesco, Catarina Oliveira, Florbela Soares, Paulo J Gavaia, María T Dinis, Elsa Cabrita
Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed...
2017: PloS One
https://www.readbyqxmd.com/read/29053583/body-weight-cycling-with-identical-diet-composition-does-not-affect-energy-balance-and-has-no-adverse-effect-on-metabolic-health-parameters
#15
Inge F Palm, Rianne G A E Schram, Hans J M Swarts, Evert M van Schothorst, Jaap Keijer
BACKGROUND: Body weight (BW) cycling, the yo-yo effect, is generally thought to have adverse effects on human metabolic health. However, human and animal experiments are limited in number and do not provide clear answers, partly due to large variations in experimental design, parameters measured, and definitions of BW cycling. Here, we examined the effect of repetitive BW cycling versus single- and non-cycling control groups, without alterations in diet composition, on steady state BW and metabolic parameters...
October 20, 2017: Nutrients
https://www.readbyqxmd.com/read/29053574/symptoms-during-adolescents-first-use-of-cigarettes-and-e-cigarettes-a-pilot-study
#16
May S Chen, Marissa G Hall, Humberto Parada, Kathryn Peebles, Kaitlyn E Brodar, Noel T Brewer
Symptoms adolescents experience during their first time using a cigarette predict their current use, but little is known regarding the symptoms experienced during first e-cigarette use. We conducted a pilot study to understand the symptoms adolescents experience when they first tried cigarettes and e-cigarettes and the associations between these symptoms and current use. Participants were 41 adolescents in two U.S. cities who had tried cigarettes or e-cigarettes. We asked adolescents to recall the symptoms they experienced during their first cigarette or e-cigarette and categorized symptoms as negative (felt bad, coughing/chest pain, bad taste, upset stomach, dizzy/lightheaded) or positive (felt relaxed, rush/buzz)...
October 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29053558/in-vivo-confocal-microscopy-reveals-corneal-reinnervation-after-treatment-of-neurotrophic-keratopathy-with-corneal-neurotization
#17
Simon S M Fung, Joseph Catapano, Uri Elbaz, Ronald M Zuker, Gregory H Borschel, Asim Ali
PURPOSE: To document the presence and location of new sensory nerve fibers after corneal neurotization using in vivo confocal microscopy (IVCM) in 2 patients with neurotrophic keratopathy (NK). METHODS: Two patients with unilateral advanced NK received corneal neurotization to surgically reinnervate the cornea. IVCM was used to identify subbasal nerve fibers and document corneal reinnervation. In 1 patient (case 1), IVCM was performed before and after corneal neurotization; in the second patient (case 2), IVCM was performed after neurotization and corneal transplantation...
October 17, 2017: Cornea
https://www.readbyqxmd.com/read/29053406/super-resolution-nanoscopy-imaging-applied-to-dna-double-strand-breaks
#18
Sofia D'Abrantes, Sarah Gratton, Pamela Reynolds, Verena Kriechbaumer, Joseph McKenna, Stephen Barnard, Dave Clarke, Stanley W Botchway
Genomic deoxyribonucleic acid (DNA) is continuously being damaged by endogenous processes such as metabolism or by exogenous events such as radiation. The specific phosphorylation of histone H2AX on serine residue 139, described as γ-H2AX, is an excellent indicator or marker of DNA double-strand breaks (DSBs). The yield of γ-H2AX (foci) is shown to have some correlation with the dose of radiation or other DSB-causing agents. However, there is some discrepancy in the DNA DSB foci yield among imaging and other methods such as gel electrophoresis...
October 20, 2017: Radiation Research
https://www.readbyqxmd.com/read/29053391/drinking-refusal-self-efficacy-and-intended-alcohol-consumption-during-a-mass-attended-youth-event
#19
Michelle I Jongenelis, Simone Pettigrew, Nicole Biagioni
BACKGROUND: Mass-attended youth events represent a substantial public health challenge due to high levels of alcohol consumption and corresponding high rates of alcohol-related harm. Although previous research has documented the protective effect of high drinking refusal self-efficacy (DRSE) on alcohol consumption in general, there is a lack of research examining the role of DRSE in reducing consumption during mass-attended youth events and the factors associated with DRSE in these contexts...
October 20, 2017: Substance Use & Misuse
https://www.readbyqxmd.com/read/29053356/the-evolution-of-clutch-size-in-hosts-of-avian-brood-parasites
#20
Iliana Medina, Naomi E Langmore, Robert Lanfear, Hanna Kokko
Coevolution with avian brood parasites shapes a range of traits in their hosts, including morphology, behavior, and breeding systems. Here we explore whether brood parasitism is also associated with the evolution of host clutch size. Several studies have proposed that hosts of highly virulent parasites could decrease the costs of parasitism by evolving a smaller clutch size, because hosts with smaller clutches will lose fewer progeny when their clutch is parasitized. We describe a model of the evolution of clutch size, which challenges this logic and shows instead that an increase in clutch size (or no change) should evolve in hosts...
November 2017: American Naturalist
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