keyword
MENU ▼
Read by QxMD icon Read
search

Incidence assay

keyword
https://www.readbyqxmd.com/read/29232415/serological-detection-of-tick-borne-relapsing-fever-in-texan-domestic-dogs
#1
Maria D Esteve-Gasent, Chloe B Snell, Shakirat A Adetunji, Julie Piccione
Tick-Borne Relapsing Fever (TBRF) is caused by spirochetes in the genus Borrelia. Very limited information exists on the incidence of this disease in humans and domestic dogs in the United States. The main objective of this study is to evaluate exposure of dogs to Borrelia turicatae, a causative agent of TBRF, in Texas. To this end, 878 canine serum samples were submitted to Texas A&M Veterinary Medical Diagnostic Laboratory from October 2011 to September 2012 for suspected tick-borne illnesses. The recombinant Borrelial antigen glycerophosphodiester phosphodiesterase (GlpQ) was expressed, purified, and used as a diagnostic antigen in both ELISA assays and Immunoblot analysis...
2017: PloS One
https://www.readbyqxmd.com/read/29230175/the-combined-use-of-in-silico-in-vitro-and-in-vivo-analyses-to-assess-anti-cancerous-potential-of-a-bioactive-compound-from-cyanobacterium-nostoc-sp-mgl001
#2
Niveshika, Ekta Verma, Shashank K Maurya, Rajnikant Mishra, Arun K Mishra
Escalating incidences of cancer, especially in developed and developing countries, demand evaluation of potential unexplored natural drug resources. Here, anticancer potential of 9-Ethyliminomethyl-12-(morpholin-4-ylmethoxy)-5,8,13,16-tetraaza -hexacene-2,3-dicarboxylic acid (EMTAHDCA) isolated from fresh water cyanobacterium Nostoc sp. MGL001 was screened through in silico, in vitro, and in vivo studies. For in silico analysis, EMTAHDCA was selected as ligand and 11 cancer related proteins (Protein Data Bank ID: 1BIX, 1NOW, 1TE6, 2RCW, 2UVL, 2VCJ, 3CRY, 3HQU, 3NMQ, 5P21, and 4B7P) which are common targets of various anticancer drugs were selected as receptors...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29229505/action-mechanism-of-naphthofuranquinones-against-fluconazole-resistant-candida-tropicalis-strains-evidenced-by-proteomic-analysis-the-role-of-increased-endogenous-ros
#3
João Batista de Andrade Neto, Cecília Rocha da Silva, Rosana de Sousa Campos, Francisca Bruna Stefany Aires do Nascimento, Letícia Serpa Sampaio, Anderson Ramos da Silva, Maria Aparecida Alexandre Josino, Manoel Odorico de Moraes, Marina Duarte Pinto Lobo, Frederico Bruno Mendes Batista Moreno, Ana Cristina de Oliveira Monteiro Moreira, Renato de Azevedo Moreira, Thalles Barbosa Grangeiro, Eufrânio Nunes da Silva Júnior, Hemerson Iuri Ferreira Magalhães, Danilo Damasceno Rocha, Bruno Coêlho Cavalcanti, Hélio Vitoriano Nobre Júnior
The increased incidence of candidemia in terciary hospitals worldwide and the cross-resistance frequency require the new therapeutic strategies development. Recently, our research group demonstrated three semi-synthetic naphthofuranquinones (NFQs) with a significant antifungal activity in a fluconazole-resistant (FLC) C. tropicalis strain. The current study aimed to investigate the action's preliminary mechanisms of NFQs by several standardized methods such as proteomic and flow cytometry analyzes, comet assay, immunohistochemistry and confocal microscopy evaluation...
December 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29227467/evidence-for-rise-in-meningococcal-serogroup-c-bactericidal-antibody-titers-in-the-absence-of-booster-vaccination-in-previously-vaccinated-children
#4
Al-Mamoon Badahdah, Ameneh Khatami, Mohamed Tashani, Harunor Rashid, Robert Booy
BACKGROUND: The introduction of meningococcal serogroup C (MenC) conjugate (MCC) vaccines in the UK and Australia led to an impressive decline in the incidence of invasive disease. This study examined bactericidal antibody titres over time in UK and Australian children who received a MCC vaccine in early childhood to test the hypothesis that ongoing boosting of immunity in the absence of further doses of vaccine in some children may contribute to ongoing protection from disease. METHODS: Serum bactericidal assay using rabbit complement (rSBA) titres at each follow-up visit were compared to all preceding visits to identify any ≥4-fold rise in titres...
December 8, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29227131/a-serosurvey-of-bluetongue-and-epizootic-haemorrhagic-disease-in-a-convenience-sample-of-sheep-and-cattle-herds-in-zimbabwe
#5
Stuart J G Gordon, Charlotte Bolwell, Chris W Rogers, Godfrey Musuka, Patrick Kelly, Alan Guthrie, Philip S Mellor, Chris Hamblin
A convenience sample of sheep and cattle herds around the cities of Harare, Kwekwe and Bulawayo, located in the Highveld region of Zimbabwe, was used to estimate the seroprevalence and sero-incidence of bluetongue virus (BTV) and epizootic haemorrhagic disease virus (EHDV) antibodies. A competitive enzyme-linked immunosorbent assay was used to identify serum antibodies against BTV and EHDV across three rainy seasons. The median sero-prevalence of BTV and EHDV antibodies in cattle was 62% (interquartile range [IQR]: 30-89) and 56% (IQR: 5-77), respectively...
November 14, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/29223963/field-evaluation-of-two-point-of-care-tests-for-syphilis-among-men-who-have-sex-with-men-verona-italy
#6
Antonella Zorzi, Maddalena Cordioli, Lorenzo Gios, Paola Del Bravo, Igor Toskin, Rosanna W Peeling, Karel Blondeel, Giuseppe Cornaglia, James Kiarie, Ronald Ballard, Massimo Mirandola
OBJECTIVES: The incidence of HIV and syphilis among men who have sex with men (MSM) in Europe has recently increased. Rapid point-of-care tests (POCTs) for syphilis can improve access to screening. The purpose of this study was to evaluate the performance of two syphilis POCTs compared with laboratory tests among MSM. METHODS: The study was undertaken in Verona, Italy. Asymptomatic MSM, potentially exposed to syphilis, were enrolled prospectively. The POCTs evaluated were SD Bioline Syphilis 3...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29222745/mir-26a-functions-as-a-tumor-suppressor-in-ambient-particulate-matter-bound-metal-triggered-lung-cancer-cell-metastasis-by-targeting-lin28b-il6-stat3-axis
#7
Yan-Yang Lu, Yi Lin, Dong-Xiao Ding, Shu Su, Qiao-Qiao Chi, You-Chi Zhang, Jian Sun, Xu Zhang, Hui-Min Zhu, Qian-Sheng Huang, Yu-Lang Chi, Guo-Zhu Ye, Shu Tao, Si-Jun Dong
Exposure to ambient particulate matter (PM) has been linked to the increasing incidence and mortality of lung cancer, but the principal toxic components and molecular mechanism remain to be further elucidated. In this study, human lung adenocarcinoma A549 cells were treated with serial concentrations of water-extracted PM10 (WE-PM10) collected from Beijing, China. Our results showed that exposure to 25 and 50 μg/ml of WE-PM10 for 48 h significantly suppressed miR-26a to upregulate lin-28 homolog B (LIN28B), and in turn activated interleukin 6 (IL6) and signal transducer and activator of transcription 3 (STAT3) in A549 cells, subsequently contributing to enhanced epithelial-mesenchymal transition and accelerated migration and invasion...
December 8, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29222255/non-chemotherapy-drug-induced-neutropenia-key-points-to-manage-the-challenges
#8
REVIEW
Brian R Curtis
Non-chemotherapy idiosyncratic drug-induced neutropenia (IDIN) is a relatively rare but potentially fatal disorder that occurs in susceptible individuals, with an incidence of 2.4 to 15.4 cases per million population. Affected patients typically experience severe neutropenia within several weeks to several months after first exposure to a drug, and mortality is ∼5%. The drugs most frequently associated with IDIN include metamizole, clozapine, sulfasalazine, thiamazole, carbimazole, amoxicillin, cotrimoxazole, ticlopidine, and valganciclovir...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29217880/renal-manifestation-in-scrub-typhus-during-a-major-outbreak-in-central-nepal
#9
A Sedhain, G R Bhattarai
Renal involvement and acute kidney injury (AKI) are common clinical manifestations seen in scrub typhus, a vector-borne tropical disease. There are no data on renal manifestation in scrub typhus in Nepal. We conducted a prospective study to analyze the incidence, urinary abnormalities, course, severity, outcome, and the predictors of AKI in patients with scrub typhus during a major outbreak in Central Nepal. Total 1398 patients admitted with acute febrile illness were subjected for Scrub Typhus Detect™ Immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) test, of which 502 (35...
November 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/29216556/influence-of-water-activity-and-temperature-on-growth-and-fumonisin-production-by-fusarium-proliferatum-strains-on-irradiated-wheat-grains
#10
Eugenia Cendoya, María Del Pilar Monge, Stella Maris Chiacchiera, María Cecilia Farnochi, María Laura Ramirez
Wheat is the most important cereal consumed by the Argentine population. In previous studies performed in durum and common wheat grains in this country it has been observed fumonisin contamination as well as high incidence of Fusarium proliferatum. Fumonisins are toxic fungal metabolites, and consumption of fumonisin-contaminated maize has been epidemiologically associated with oesophageal cancer and neural tube defects in some human populations. Using irradiated wheat-grains, the effects of abiotic factors, temperature (15, 25, and 30°C) and water activity (aW; 0...
December 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29215841/-histamine-metabolism-disorder-in-pathogenesis-of-chronic-pelvic-pain-in-patients-with-external-genital-endometriosis
#11
M R Orazov, V Y Radzinskiy, M B Khamoshina, E N Nosenko, E S Tokaeva, L K Barsegyan, Y R Zakirova
Objective. To study features of histamine metabolism in patients with chronic pelvic pain associated with external genital endometriosis. Methods. For quantitative assessment of histamine level in peripheral blood was taken from 100 patients which than was centrifuged. In blood serum histamine concentration was determined by enzyme-linked immunosorbent assay method with reagents «Histamine ЕLISA» on the machine BAE-1000 Histamine (Labor Diagnostika Nord - LDN, Hermany). A pain syndrome was assessed by Visual Analog Scale (VAS), quality of life assessment - by Endometriosis Health Profile Questionnaire (EHR-30), level of anxiety was determined by Spielberger-Khanin questionnaire...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
https://www.readbyqxmd.com/read/29215785/expression-and-functional-characterization-of-two-natural-heparin-binding-site-variants-of-antithrombin
#12
Peyman Dinarvand, Likui Yang, Bruno O Villoutreix, Alireza R Rezaie
BACKGROUND: Several heparin-binding site (HBS) variants of antithrombin (AT) have been identified which predispose carriers to higher incidence of thrombosis. The cause of thrombosis in carriers of HBS variants has been primarily attributed to a loss in their heparin-dependent anticoagulant function. OBJECTIVE: The objective of this study was to determine whether HSB mutations affect the anti-inflammatory functions of variants. METHODS: Two HBS variants of AT (AT-I7N and AT-L99F), which are known to be associated with higher incidence of thrombosis, were expressed in mammalian cells and purified to homogeneity...
December 7, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29212443/the-current-incidence-prevalence-and-residual-risk-of-hepatitis-b-viral-infections-among-voluntary-blood-donors-in-china
#13
Ling Li, Tingting Han, Liang Zang, Libin Niu, Weifang Cheng, Hongkeng Lin, Ka Yi Li, Ruan Cao, Binghai Zhao, Yuqiang Liu, Guojin Ou, Xiao Liu, Yingjie Qi, Yanhua Li, Zhong Liu
BACKGROUND: There are few data available on the prevalence, incidence, and residual risk of transfusion-transmitted HBV (TT-HBV) infections among Chinese blood donors. This study investigated the demographic characteristics of blood donors, as well as the prevalence, incidence, and residual risk (RR) of TT-HBV infections in six large blood centers in different regions of China. METHODS: The demographic characteristics and HBV screening test results of blood donors from six blood centers in different regions in China were collected and analyzed...
December 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29211104/surveillance-of-seroepidemiology-and-morbidity-of-chagas-disease-in-the-negro-river-brazilian-amazon
#14
José Rodrigues Coura, Angela Cv Junqueira, João Marcos Bb Ferreira
BACKGROUND: Chagas disease in the Brazilian Amazon Region was previously regarded as an enzootic disease of wild animals. More recently, in situations where humans have penetrated the wild ecotope or where triatomines and/or wild animals (marsupials) have invaded human homes resulting in disease transmission, Chagas disease has come to be regarded as an anthropozoonosis. We found that the highest incidence of infection due to Trypanosoma cruzi and Chagas disease occurred among piassaba fibre gatherers and their families...
January 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29209406/serum-prolactin-levels-and-sexual-dysfunction-in-patients-with-schizophrenia-treated-with-antipsychotics-comparison-between-aripiprazole-and-other-atypical-antipsychotics
#15
Eiji Kirino
Objectives: Antipsychotics, even atypical ones, can induce hyperprolactinemia. Aripiprazole (APZ), a dopamine D2 partial agonist, has a unique pharmacological profile and few side effects. We investigated the incidence of hyperprolactinemia in patients with schizophrenia treated with APZ and other antipsychotics. Methods: Serum prolactin levels were measured by ELISA (enzyme-linked immunosorbent assay). A questionnaire survey was used to evaluate subjective sexual dysfunction...
2017: Annals of General Psychiatry
https://www.readbyqxmd.com/read/29209098/the-distribution-frequency-of-interferon-gamma-receptor-1-gene-polymorphisms-in-interferon-%C3%AE-release-assay-positive-patients
#16
Changguo Chen, Lei Chen, Changwei Chen, Qiuyuan Chen, Qiangyuan Zhao, Youyou Dong
Tuberculosis is caused by mycobacterium, a potentially fatal infectious bacterium. In recent years, TB cases increased in the whole world. WHO statistics data shows that the world's annual tuberculosis incidence was 8~10 million with about 3 million deaths. Several studies have shown that susceptibility to tuberculosis may be associated with IFNGR1 gene polymorphisms. Here, we report the distribution frequency of IFNGR1 gene polymorphisms in 103 cases of IGA-negative patients and 100 cases of IGA-positive patients from China by sequencing the IFNGR1 proximal ~750 bp promoter region...
2017: Disease Markers
https://www.readbyqxmd.com/read/29207978/potential-therapeutic-effects-of-n-butylidenephthalide-from-radix-angelica-sinensis-danggui-in-human-bladder-cancer-cells
#17
Sheng-Chun Chiu, Tsung-Lang Chiu, Sung-Ying Huang, Shu-Fang Chang, Shee-Ping Chen, Cheng-Yoong Pang, Teng-Fu Hsieh
BACKGROUND: N-butylidenephthalide (BP) isolated from Radix Angelica Sinensis (Danggui) exhibits anti-tumorigenic effect in various cancer cells both in vivo and in vitro. The effect of BP in bladder cancer treatment is still unclear and worth for further investigate. METHODS: Changes of patients with bladder cancer after Angelica Sinensis exposure were evaluated by analysis of Taiwan's National Health Insurance Research Database (NHIRD) database. The anti-proliferative effect of BP on human bladder cancer cells was investigated and their cell cycle profiles after BP treatment were determined by flow cytometry...
December 6, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29207608/gsta1-diplotypes-affect-busulfan-clearance-and-toxicity-in-children-undergoing-allogeneic-hematopoietic-stem-cell-transplantation-a-multicenter-study
#18
Marc Ansari, Patricia Huezo-Diaz Curtis, Chakradhara Rao S Uppugunduri, Mohammed Aziz Rezgui, Tiago Nava, Vid Mlakar, Laurence Lesne, Yves Théoret, Yves Chalandon, Lee L Dupuis, Tao Schechter, Imke H Bartelink, Jaap J Boelens, Robbert Bredius, Jean-Hugues Dalle, Saba Azarnoush, Petr Sedlacek, Victor Lewis, Martin Champagne, Christina Peters, Henrique Bittencourt, Maja Krajinovic
Busulfan (BU) dose adjustment following therapeutic drug monitoring contributes to better outcome of hematopoietic stem cell transplantation (HSCT). Further improvement could be achieved through genotype-guided BU dose adjustments. To investigate this aspect, polymorphism within glutathione S transferase genes were assessed. Particularly, promoter haplotypes of the glutathione S transferase A1 (GSTA1) were evaluated in vitro, with reporter gene assays and clinically, in a pediatric multi-center study (N =138) through association with BU pharmacokinetics (PK) and clinical outcomes...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207113/spontaneous-hypothermia-ameliorated-inflammation-and-neurologic-deficit-in-rat-cardiac-arrest-models-following-resuscitation
#19
Minggen Zhou, Peng Wang, Zhengfei Yang, Haidong Wu, Zitong Huan
Cardiac arrest (CA) is a leading cause of mortality worldwide. The majority of the associated mortalities are caused by post‑CA syndrome, which includes symptoms, such as neurologic damage, myocardial dysfunction and systemic inflammation. Following CA, return of spontaneous circulation (ROSC) leads to a brain reperfusion injury, which subsequently causes adverse neurologic outcomes or mortality. Therefore, investigating the underlying mechanisms of ROSC‑induced neurologic deficits and establishing potential treatments is critical to prevent and treat post‑CA syndrome...
November 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29203554/the-clinical-and-prognostic-relevance-of-driver-mutations-in-203-taiwanese-patients-with-primary-myelofibrosis
#20
Ming-Chung Kuo, Tung-Huei Lin, Chien-Feng Sun, Tung-Liang Lin, Jin-Hou Wu, Po-Nan Wang, Ying-Jung Huang, Hung Chang, Ting-Yu Huang, Lee-Yung Shih
AIMS: We investigated the clinical and prognostic relevance of the mutational status of driver genes with allele burden and endogenous erythroid colony (EEC) growth in 203 Taiwanese patients with primary myelofibrosis (PMF). METHODS: Pyrosequencing was used to detect JAK2V617F mutational status and measure allele burden, while MPL (exon 10) mutations were analysed by PCR assay and then by direct sequencing. CALR exon 9 mutations were first screened for length changes by GeneScan followed by sequencing...
December 4, 2017: Journal of Clinical Pathology
keyword
keyword
39525
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"