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https://www.readbyqxmd.com/read/29042988/toll-like-receptor-2-and-dectin-1-function-as-promising-biomarker-for-aspergillus-fumigatus-infection
#1
Peng-Peng Zhang, Xiao-Feng Xin, Xiao-Yong Xu, Li-Ping Fang, Jie Wu, Yi Shi
In recent years, along with the wide application of organ transplantation and immunosuppressive agents, as well as the abuse of broad spectrum antibiotics, the incidence of invasive fungal infections has been increasing gradually. The present study aimed to identify novel biomarkers in cells infected with Aspergillus fumigatus. Human umbilical vein endothelial cells (HUVECs) were infected with Aspergillus fumigatus and then harvested at different time-points (0, 1, 2, 4 and 6 h). The expression Toll-like receptor 2 (TLR2) and dectin-1 expression were examined using flow cytometry and western blotting, and fluorescence-based microscopy was used to evaluate their distribution...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042141/involvement-of-bmi-1-gene-in-the-development-of-gastrointestinal-stromal-tumor-by-regulating-p16-ink4a-p14-arf-gene-expressions-an-in-vivo-and-in-vitro-study
#2
Jiang-Li Wang, Jiang-Hong Wu, Cai Hong, Ya-Nong Wang, Ye Zhou, Zi-Wen Long, Ying Zhou, Hai-Shu Qin
OBJECTIVE: This study was conducted in order to explore the role that Bmi-1 plays during the development of a gastrointestinal stromal tumor (GIST) by regulation of the p16(Ink4A) and p14(ARF) expressions. METHODS: Eighty-six patients diagnosed with GIST were selected to take part in this experiment. The Bmi-1 protein expressions in GIST and adjacent normal tissues were detected using immunohistochemistry and further analyzed by using photodensitometry. To monitor and track the progression of the GIST, a 3-year follow-up was conducted for all affected patients...
September 18, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29037218/clinicopathologic-features-and-treatment-efficacy-of-chinese-patients-with-braf-mutated-metastatic-colorectal-cancer-a-retrospective-observational-study
#3
Xicheng Wang, Qing Wei, Jing Gao, Jian Li, Jie Li, Jifang Gong, Yanyan Li, Lin Shen
BACKGROUND: The prognostic role of the V600E mutation of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) in metastatic colorectal cancer (mCRC) is well established, but the therapeutic regimen targeting this disease is lacking. This study aimed to analyze the clinicopathologic features of and treatment efficacy of commonly used regimens on BRAF-mutated mCRCs. METHODS: We collected and reviewed the medical records of mCRC patients treated at Peking University Cancer Hospital & Institute (Beijing, China) between July 2011 and July 2016...
October 16, 2017: Chinese Journal of Cancer
https://www.readbyqxmd.com/read/29035128/prospective-study-of-plasma-homocysteine-its-dietary-determinants-and-risk-of-age-related-macular-degeneration-in-men
#4
William G Christen, Nancy R Cook, Stephanie E Chiuve, Paul M Ridker, J Michael Gaziano
PURPOSE: Cross-sectional and case-control studies generally support a direct association between elevated plasma homocysteine and age-related macular degeneration (AMD), but data from prospective studies are limited. We examined the prospective relation of plasma homocysteine level, its dietary determinants, and risk of AMD in a large cohort of apparently healthy male physicians. METHODS: During a mean follow-up of 11.2 years, we identified 146 incident cases of visually significant AMD (responsible for a reduction of visual acuity to 20/30 or worse), and 146 controls matched for age, smoking status, and time of blood draw...
October 16, 2017: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/29033349/a-polymorphism-in-the-glucocorticoid-receptor-gene-is-associated-with-refractory-hypotension-in-premature-infants
#5
Kei Ogasawara, Maki Sato, Koichi Hashimoto, Takashi Imamura, Hayato Go, Mitsuaki Hosoya
BACKGROUND: Glucocorticoids play an important role in endocrine control. The association of glucocorticoid receptor (GR) gene polymorphisms with altered sensitivity to glucocorticoid therapy has been reported in adults. However, there are few such reports in infants. The present study analyzed the prevalence of four GR polymorphisms in preterm infants born before 30 weeks of gestation and determined the associations between these polymorphisms and clinical outcomes in the infants. METHODS: Totally, 41 preterm infants born at two hospitals in Fukushima were retrospectively screened for the presence of four GR gene polymorphisms, using a TaqMan single-nucleotide polymorphism genotyping assay...
September 27, 2017: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/29031892/altered-angiogenesis-as-a-common-mechanism-underlying-preterm-birth-small-for-gestational-age-and-stillbirth-in-women-living-with-hiv
#6
Andrea L Conroy, Chloe R Mcdonald, Joel L Gamble, Peter Olwoch, Paul Natureeba, Deborah Cohan, Moses R Kamya, Diane V Havlir, Grant Dorsey, Kevin C Kain
BACKGROUND: Angiogenic processes in the placenta are critical regulators of fetal growth and impact birth outcomes, but there are limited data documenting these processes in HIV-infected women or women from low-resource settings. OBJECTIVE: To determine whether angiogenic factors are associated with adverse birth outcomes in HIV-infected pregnant women started on antiretroviral therapy. STUDY DESIGN: This is a secondary analysis of samples collected as part of a clinical trial randomizing pregnant women infected with HIV to lopinavir/ritonavir (n=166) or efavirenz-based (n=160) antiretroviral therapy in Tororo, Uganda...
October 11, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29028095/bcar4-increase-cisplatin-resistance-and-predicted-poor-survival-in-gastric-cancer-patients
#7
L Wang, Q Chunyan, Y Zhou, Q He, Y Ma, Y Ga, X Wang
OBJECTIVE: Gastric cancer is a common malignancy with increasing worldwide incidence, and chemotherapeutic drugs for gastric cancer are not effective. Long non-coding RNA (lncRNA) has been proved to be important in different cancer progression. In this research, we investigated whether lncRNAs have relations with drug resistance in gastric cancer to find new potential targets for therapy, which can increase the survival time of the drug-resistant gastric patient. PATIENTS AND METHODS: qRT-PCR was used to detect the expression of BCAR4 in 113 cases of gastric cancer tissue and adjacent tissue, and the clinical significance was also analyzed...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29027886/molecular-typing-of-bois-noir-phytoplasma-strains-in-the-chianti-classico-area-tuscany-central-italy-and-their-association-with-symptom-severity-in-vitis-vinifera-l-cv-sangiovese
#8
Roberto Pierro, Alessandro Passera, Alessandra Panattoni, Paola Casati, Andrea Luvisi, Domenico Rizzo, Piero Attilio Bianco, Fabio Quaglino, Alberto Materazzi
Bois Noir (BN) is the most widespread disease of the grapevine yellows complex in the Euro-Mediterranean area. BN is caused by 'Candidatus Phytoplasma solani' (BNp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. In this study, genetic diversity among BNp strains, their prevalence and possible association with grapevine symptom severity were investigated in a Sangiovese clone organic vineyard, in the Chianti Classico area (Tuscany). Field surveys over two years revealed a range of symptom severity on grapevine and an increase of BN incidence...
October 13, 2017: Phytopathology
https://www.readbyqxmd.com/read/29025913/analyses-of-the-francecoag-cohort-support-immunogenicity-differences-among-one-plasma-derived-and-two-recombinant-factor-viii-brands-in-boys-with-severe-hemophilia-a
#9
Thierry Calvez, Hervé Chambost, Roseline d'Oiron, Vincent Dalibard, Virginie Demiguel, Alexandra Doncarli, Yves Gruel, Yoann Huguenin, Patrick Lutz, Chantal Rothschild, Christine Vinciguerra, Jenny Goudemand
Around one third of boys with severe hemophilia A develop inhibitors (neutralizing antibodies) against their therapeutic factor VIII product. This adverse effect may result in more life-threatening bleeding, disability, impaired quality of life, and costly care. We compared the inhibitor incidence in boys treated with the three factor VIII products most used in France: one plasma-derived (Factane) and two recombinant products (Advate and Kogenate Bayer). A previously untreated patient cohort was created in 1994 to investigate risk factors for inhibitor development...
October 12, 2017: Haematologica
https://www.readbyqxmd.com/read/29025375/dopamine-induced-sult1a3-4-promotes-emt-and-cancer-stemness-in-hepatocellular-carcinoma
#10
Juan Zou, Hong Li, Qianling Huang, Xiaomin Liu, Xiaoxiao Qi, Ying Wang, Linlin Lu, Zhongqiu Liu
Hepatocellular carcinoma has the second highest incidence rate among malignant cancers in China. Hepatocellular carcinoma development is complex because of the metabolism disequilibrium involving SULT1A3/4, a predominant sulfotransferase that metabolizes sulfonic xenobiotics and endogenous catecholamines. However, the correlation between SULT1A3/4 and hepatocellular carcinoma progression is unclear. By utilizing immunofluorescence and immunohistochemical analysis, we found that in nine hepatocellular carcinoma clinical specimens, SULT1A3/4 was abundantly expressed in tumor tissues compared to that in the adjacent tissues...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29021105/invasive-pneumococcal-pneumonia-caused-by-13-valent-pneumococcal-conjugate-vaccine-types-in-children-with-different-schedules
#11
Hong-Yi Lee, Yu-Chia Hsieh, Ching-Chuan Liu, Yi-Chuan Huang, Kuang-Yi Chang, Hsin Chi, Luan-Yin Chang, Yhu-Chering Huang, Li-Min Huang
BACKGROUND: In Taiwan, the age group with the greatest incidence of invasive pneumococcal disease is 2-5 years of age, which is different from other countries. This study was conducted to identify risk factors and different 13-valent pneumococcal conjugate vaccine (PCV13) schedules associated with vaccine-type invasive pneumococcal pneumonia (IPP) despite prior vaccination. METHODS: A case-control study was conducted prospectively between August 2012 and December 2015 at five participating medical centers...
September 27, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29020987/shcbp1-promotes-synovial-sarcoma-cell-metastasis-via-targeting-tgf-%C3%AE-1-smad-signaling-pathway-and-is-associated-with-poor-prognosis
#12
Changliang Peng, Hui Zhao, Yan Song, Wei Chen, Xiaoying Wang, Xiaoli Liu, Cheng Zhang, Jie Zhao, Ji Li, Guanghui Cheng, Dongjin Wu, Chunzheng Gao, Xiuwen Wang
BACKGROUND: Our previous studies reported that SHC SH2-domain binding protein 1 (SHCBP1) functions as an oncogene via promoting cell proliferations in synovial sarcoma (SS) cells. However, whether SHCBP1 has any effect on tumor metastasis remains unexplored. METHODS: The expression of SHCBP1 was analyzed in 76 SS tissues and two SS cell lines by immunohistochemistry and real-time RT-PCR. The relationship between SHCBP1 expression and the clinicopathological features of SS was investigated...
October 11, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29020191/quantiferon%C3%A2-tb-gold-in-tube-as-a-confirmatory-test-for-tuberculin-skin-test-in-tuberculosis-contact-tracing-a-non-inferiority-clinical-trial
#13
Laura Muñoz, Miguel Santin, Fernando Alcaide, M J Ruíz-Serrano, Paloma Gijón, Elena Bermúdez, Angel Domínguez-Castellano, M D Navarro, Encarnación Ramírez, Elvira Pérez-Escolano, M D López-Prieto, José Gutiérrez-Rodriguez, Luis Anibarro, Laura Calviño, Matilde Trigo, Carmen Cifuentes, Mercedes García-Gasalla, Antoni Payeras, Oriol Gasch, Mateu Espasa, Ramon Agüero, Diego Ferrer, Xavier Casas, Araceli González-Cuevas, Alberto García-Zamalloa, Edurne Bikuña, María Lecuona, Rosa Galindo, Marta Ramírez-Lapausa, Raquel Carrillo
Background: Screening strategies based on interferon-γ release assays in tuberculosis contact tracing may reduce the need for preventive therapy without increasing subsequent active disease. Methods: We conducted an open-label, randomized trial, to test the non-inferiority of a two-step strategy with the tuberculin skin test followed by QuantiFERON®-TB Gold In-Tube as a confirmatory test (the TST/QFT arm) to the standard TST-alone strategy (TST arm) for targeting preventive therapy in household contacts of tuberculosis...
August 18, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29019333/evaluation-of-brca1-large-genomic-rearrangements-in-group-of-egyptian-female-breast-cancer-patients-using-mlpa
#14
Ola M Eid, Eman A El Ghoroury, Maha M Eid, Rana M Mahrous, Mohamed I Abdelhamid, Zahra I Aboafya, Esmat A Abdel Ghaffar, Amany H Abdelrahman
INTRODUCTION: Breast cancer is one of the most widespread cancers affecting women all over the world. In Egypt, it is considered to be the first cause of malignancies among female. BRCA1 Large Genomic Rearrangements (LGRs) have been reported in hereditary breast families and occurs in considerable proportion of cases in various populations. OBJECTIVE AND METHODS: We investigated the incidence of BRCA1 LGRs in group of Egyptian females with breast cancer using Multiplex Ligation-dependent Probe Amplification (MLPA) assay...
September 2017: Gulf Journal of Oncology
https://www.readbyqxmd.com/read/29017920/mycophenolic-mofetil-an-alternative-antiviral-and-immunomodulator-for-the-highly-pathogenic-avian-influenza-h5n1-virus-infection
#15
Junhyung Cho, Hwajung Yi, Eun Young Jang, Mi-Seon Lee, Joo-Yeon Lee, Chun Kang, Chan Hee Lee, Kisoon Kim
Infection with the highly pathogenic avian influenza H5N1 virus results in a high incidence of mortality in humans. Severe complications from infection are often associated with hypercytokinemia. However, current neuraminidase inhibitors (NAIs) have several limitations including the appearance of oseltamivir-resistant H5N1 virus and the inability to completely ameliorate hyper-immune responses. To overcome these limitations, we evaluated the anti-viral activity of mycophenolic mofetil (MMF) against A/Vietnam/1194/2004 (H5N1) virus infection using MDCK cells and mice...
October 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29016903/screening-for-latent-tuberculosis-in-uk-health-care-workers
#16
M Henderson, S J Howard
Background: Active tuberculosis (TB) infection was diagnosed in two health care workers (HCWs) originally from high-incidence countries at a National Health Service (NHS) hospital trust in Northern England. In response, the trust screened current clinical employees from countries with a high TB prevalence for active and latent TB infection (LTBI). Aims: To identify the number of HCWs, within the organization, who are at risk of TB infection. Methods: Clinical employees from countries with a high TB prevalence (those described by the World Health Organization as having an incidence of >40 cases per 100000 populations) were reviewed...
August 9, 2017: Occupational Medicine
https://www.readbyqxmd.com/read/29016844/pik3cb-p110%C3%AE-is-a-selective-survival-factor-for-glioblastoma
#17
Kevin J Pridham, Lamvy Le, Sujuan Guo, Robin T Varghese, Sarah Algino, Yanping Liang, Renee Fajardin, Cara M Rodgers, Gary R Simonds, Deborah F Kelly, Zhi Sheng
Background: Glioblastoma (GBM) is difficult to treat. Phosphoinositide 3-kinase (PI3K) is an attractive therapeutic target for GBM; however, targeting this pathway to effectively treat GBM is not successful because the roles of PI3K isoforms remain to be defined. The aim of this study is to determine whether PIK3CB/p110β, but not other PI3K isoforms, is a biomarker for GBM recurrence and important for cell survival. Methods: Gene expression and clinical relevance of PI3K genes in GBM patients were analyzed using online databases...
September 16, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29016684/performance-of-the-xpert-mtb-rif-assay-for-the-diagnosis-of-pulmonary-tuberculosis-and-rifampin-resistance-in-a-low-incidence-high-resource-setting
#18
Jason P Rice, Marva Seifert, Kathleen S Moser, Timothy C Rodwell
Performance of the Xpert MTB/RIF assay, designed to simultaneously detect Mycobacterium tuberculosis complex (MTBC) and rifampin (RIF) resistance, has been well documented in low-resource settings with high TB-incidence. However, few studies have assessed its accuracy in low TB incidence settings. We evaluated the performance of Xpert MTB/RIF using clinical sputum specimens routinely collected from suspect pulmonary TB patients over a 4-year time period in San Diego County, California. Xpert MTB/RIF results were compared to acid-fast bacilli (AFB) smear microscopy, mycobacterial culture, and phenotypic drug susceptibility testing (DST)...
2017: PloS One
https://www.readbyqxmd.com/read/29016289/experimental-and-clinical-treatment-of-chagas-disease-a-review
#19
Policarpo Ademar Sales Junior, Israel Molina, Silvane Maria Fonseca Murta, Adrián Sánchez-Montalvá, Fernando Salvador, Rodrigo Correa de Oliveira, Cláudia Martins Carneiro
Chagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi that infects a broad range of triatomines and mammalian species, including man. It afflicts 8 million people in Latin America, and its incidence is increasing in nonendemic countries owing to rising international immigration and nonvectorial transmission routes such as blood donation. Since the 1960s, the only drugs available for the clinical treatment of this infection have been benznidazole and nifurtimox (NFX). Treatment with these trypanocidal drugs is recommended in both the acute and chronic phases of CD...
October 2, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28994788/evaluating-primary-blast-effects-in-vitro
#20
Niall J Logan, Hari Arora, Claire A Higgins
Exposure to blast events can cause severe trauma to vital organs such as the lungs, ears, and brain. Understanding the mechanisms behind such blast-induced injuries is of great importance considering the recent trend towards the use of explosives in modern warfare and terrorist-related incidents. To fully understand blast-induced injury, we must first be able to replicate such blast events in a controlled environment using a reproducible method. In this technique using shock tube equipment, shock waves at a range of pressures can be propagated over live cells grown in 2D, and markers of cell viability can be immediately analyzed using a redox indicator assay and the fluorescent imaging of live and dead cells...
September 18, 2017: Journal of Visualized Experiments: JoVE
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