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https://www.readbyqxmd.com/read/28922351/estimation-of-the-mechanical-properties-of-the-eye-through-the-study-of-its-vibrational-modes
#1
M Á Aloy, J E Adsuara, P Cerdá-Durán, M Obergaulinger, J J Esteve-Taboada, T Ferrer-Blasco, R Montés-Micó
Measuring the eye's mechanical properties in vivo and with minimally invasive techniques can be the key for individualized solutions to a number of eye pathologies. The development of such techniques largely relies on a computational modelling of the eyeball and, it optimally requires the synergic interplay between experimentation and numerical simulation. In Astrophysics and Geophysics the remote measurement of structural properties of the systems of their realm is performed on the basis of (helio-)seismic techniques...
2017: PloS One
https://www.readbyqxmd.com/read/28920429/hybrid-mos2-h-bn-nanofillers-as-synergic-heat-dissipation-and-reinforcement-additives-in-epoxy-nanocomposites
#2
Helio Ribeiro, João Paulo Campos Trigueiro, Wellington M Silva, Cristiano F Woellner, Peter Samora Owuor, Alin Cristian Chipara, Magnovaldo Carvalho Lopes, Chandra Sekhar Tiwary, Jairo José Pedrotti, Rodrigo Villegas Salvatierra, James M Tour, Nitin Chopra, Ihab N Odeh, Glaura Silva, Pulickel M Ajayan
Two-dimensional (2D) nanomaterials as molybdenum disulfide (MoS2), hexagonal boron nitride (h-BN), and the hybrid (MoS2/h-BN) were employed as fillers to improve the physical properties of epoxy composites. Nanocomposites were produced in different concentrations and studied in their microstructure, mechanical and thermal properties. The hybrid 2D mixture imparted efficient reinforcement to the epoxy leading to increases of up to 95% in tensile strength, 60% in ultimate strain and 58% in Young's modulus. Moreover, an enhancement of 203% in thermal conductivity was achieved for the hybrid composite as compared to neat polymer...
September 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28919010/germ-cell-proliferation-and-apoptosis-during-testicular-regression-in-a-seasonal-breeding-fish-kept-in-captivity
#3
Yves Moreira Ribeiro, Santer Alvares de Matos, Fabricio Flavio Theophilo Domingos, Helio Batista Dos Santos, Augusto Bicalho Cruz Vieira, Nilo Bazzoli, Elizete Rizzo
Cell proliferation and apoptosis regulate germ cells stock and sperm production, eliminate anomalous gametes, and are essential parameters to consider in fish farming. Herein, spermatogenic activity as well as germ cell proliferation and apoptosis were assessed in Leporinus taeniatus, a seasonal breeding species from the São Francisco River basin, Brazil. Testes of 24 adult fishes from a farming station were sampled between December and July and processed for light and transmission electron microscopy and immunohistochemistry for PCNA and TUNEL assay...
September 12, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28895517/assessment-of-molecular-and-genetic-evolution-antigenicity-and-virulence-properties-during-the-persistence-of-the-infectious-bronchitis-virus-in-broiler-breeders
#4
Filipe Santos Fernando, Thaiane Coelho Kasmanas, Priscila Diniz Lopes, Maria de Fátima da Silva Montassier, Marcos Antônio Zanella Mores, Viviane Casagrande Mariguela, Caren Pavani, Romeu Moreira Dos Santos, Mário Sérgio Assayag, Helio José Montassier
The infectious bronchitis virus (IBV) causes a highly contagious disease [infectious bronchitis (IB)] that results in substantial economic losses to the poultry industry worldwide. We conducted a molecular and phylogenetic analysis of the S1 gene of Brazilian (BR) IBV isolates from a routinely vaccinated commercial flock of broiler breeders, obtained from clinical IB episodes that occurred in 24-, 46- and 62-week-old chickens. We also characterized the antigenicity, pathogenesis, tissue tropism and spreading of three IBV isolates by experimental infection of specific pathogen-free (SPF) chickens and contact sentinel birds...
September 12, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28894332/breast-cancer-screening-updated-recommendations-of-the-brazilian-college-of-radiology-and-diagnostic-imaging-brazilian-breast-disease-society-and-brazilian-federation-of-gynecological-and-obstetrical-associations
#5
Linei Augusta Brolini Dellê Urban, Luciano Fernandes Chala, Selma di Pace Bauab, Marcela Brisighelli Schaefer, Radiá Pereira Dos Santos, Norma Medicis de Albuquerque Maranhão, Ana Lucia Kefalas, José Michel Kalaf, Carlos Alberto Pecci Ferreira, Ellyete de Oliveira Canella, João Emílio Peixoto, Heverton Leal Ernesto de Amorim, Helio Sebastião Amâncio de Camargo Junior
OBJECTIVE: To present the current recommendations for breast cancer screening in Brazil, as devised by the Brazilian College of Radiology and Diagnostic Imaging, the Brazilian Breast Disease Society, and the Brazilian Federation of Gynecological and Obstetrical Associations. MATERIALS AND METHODS: We analyzed scientific studies available in the Medline and Lilacs databases. In the absence of evidence, the recommendations reflected the consensus of a panel of experts...
July 2017: Radiologia Brasileira
https://www.readbyqxmd.com/read/28888508/functional-independence-of-children-with-myelomeningocele-is-it-associated-with-the-informal-caregivers-burden
#6
Karen Negrão Cavalari, Pedro Tadao Hamamoto Filho, Silvia Maria Caldeira, Helio Rubens de Carvalho Nunes, Fernanda Maria Alves Lima, Marla Andréia Garcia de Avila
PURPOSE: This study aims to evaluate the association between the functional independence of children after postnatal spinal dysraphism correction and informal caregivers' burden. DESIGN AND METHODS: This is a cross-sectional study conducted in a child neurosurgery clinic at a University hospital. We included informal caregivers of children between six months and seven-and-a-half years old who were operated on for spinal dysraphism correction due to myelomeningocele...
September 2017: Journal of Pediatric Nursing
https://www.readbyqxmd.com/read/28887255/forced-air-warming-in-patients-undergoing-endovascular-procedures-comparison-between-two-thermal-blanket-models
#7
Frederico L J Monteiro, Helio Halpern, Fernando Bortoli, Lucia Kataoka, Cristina Marumo, Mariângela Ribeiro, Matheus Castellassi, Cynthia Mendes, Nelson Wolosker
STUDY OBJECTIVE: The purpose of this study was to compare the efficiency between two thermal blanket models: the upper blanket and the underbody blanket in patient heating and hypothermia prevention during endovascular surgery. DESIGN: Prospective randomized study SETTING: Operating room PATIENTS: 50 patients, ASA 2-4 physical status, receiving endovascular surgery repair of infrarenal abdominal aortic aneurysm or lower limb angioplasty by endovascular technique were studied...
September 5, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28881761/transcription-factor-c-rel-is-indispensable-for-generation-of-thymic-but-not-of-peripheral-foxp3-regulatory-t-cells
#8
Maik Luu, Elena Jenike, Niyati Vachharajani, Alexander Visekruna
The transcription factor c-Rel has been shown to be crucial for development of regulatory T cells (Tregs). Recent studies have reported that the expression of transcription factor Helios in Foxp3(+) Tregs correlates with thymic origin of these cells (tTregs). Notably, we found that only the Helios(+)Foxp3(+) Treg cell population was substantially reduced in c-Rel deficient mice. In contrast to a defective tTreg development, we observed an expansion of mucosal Tregs during the induction of acute colitis in rel(-/-) mice...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28871547/a-step-by-step-protocol-for-classifying-aox-proteins-in-flowering-plants
#9
José Hélio Costa, Clesivan Pereira Dos Santos, Kátia Daniella da Cruz Saraiva, Birgit Arnholdt-Schmitt
The potential of alternative oxidase (AOX) genes to develop functional markers for plant breeding programs has been emphasized. In this sense, it is essential to have a reliable classification system, which could aid in the selection of candidate AOX genes from different species. In the case of angiosperms AOX, a robust classification system is required because this enzyme is encoded by variable gene numbers (1-6 genes) with variable AOX subfamilies and subtypes. Thus, in this protocol, we present a detailed guideline to application of a classification scheme of AOX based on specific amino acids and phylogeny...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28871546/a-driving-bioinformatics-approach-to-explore-co-regulation-of-aox-gene-family-members-during-growth-and-development
#10
José Hélio Costa, Birgit Arnholdt-Schmitt
The alternative oxidase (AOX) gene family is a hot candidate for functional marker development that could help plant breeding on yield stability through more robust plants based on multi-stress tolerance. However, there is missing knowledge on the interplay between gene family members that might interfere with the efficiency of marker development. It is common view that AOX1 and AOX2 have different physiological roles. Nevertheless, both family member groups act in terms of molecular-biochemical function as "typical" alternative oxidases and co-regulation of AOX1 and AOX2 had been reported...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28871541/procedures-of-mitochondria-purification-and-gene-expression-to-study-alternative-respiratory-and-uncoupling-pathways-in-fruits
#11
Jurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz Saraiva, Diederson Bortolino Santana, Clesivan Pereira Dos Santos, Marcos Goes Oliveira, José Hélio Costa
We describe detailed procedures to get intact and well-coupled mitochondria from a variety of fruit species such as papaya (Carica papaya), guava (Psidium guajava), tomato (Solanum lycopersicum), and strawberry (Fragaria x ananassa) as well as the protocols to assess the capacities of AOX and UCP pathways in intact fruit mitochondria. The procedures presented here were tested for the species mentioned above; their use with other types of fruits must be tested for yield of intact and active mitochondria. This is possible from individual adjustments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28871165/zoonotic-intestinal-helminths-interact-with-the-canine-immune-system-by-modulating-t-cell-responses-and-preventing-dendritic-cell-maturation
#12
Johannes Junginger, Katharina Raue, Karola Wolf, Elisabeth Janecek, Veronika M Stein, Andrea Tipold, Anne-Rose Günzel-Apel, Christina Strube, Marion Hewicker-Trautwein
Parasite co-evolution alongside the mammalian immune system gave rise to several modulatory strategies by which they prevent exaggerated pathology and facilitate a longer worm survival. As little is known about the immunoregulatory potential of the zoonotic canine parasites Ancylostoma caninum and Toxocara canis in the natural host, the present study aimed to investigate whether their larval excretory-secretory (ES) products can modulate the canine immune system. We demonstrated TcES to increase the frequency of CD4+ Foxp3(high) T cells, while both AcES and TcES were associated with elevated Helios expression in Foxp3(high) lymphocytes...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28855068/in-vivo-confocal-raman-spectroscopy-for-intrinsic-aging-and-photoaging-assessment
#13
Livia de Vasconcelos Nasser Caetano, Thiago de Oliveira Mendes, Edileia Bagatin, Helio Amante Miot, Juliana Laudiceia Marques Soares, Milvia Maria Simoes E Silva Enokihara, Airton Abrahao Martin
BACKGROUND: In vivo confocal Raman spectroscopy is a non-invasive method to assess either the epidermis or the dermis composition. Few studies have focused on dermis collagen alterations through intrinsic aging and photoaging. OBJECTIVE: This study evaluated the in vivo Raman spectra from the dermis of a photoexposed site versus a non-photoexposed region in different age groups, and evaluated the correlation between peak intensities and age, photoaging score and the amount of collagen assessed with histology and high frequency ultrasound (HFUS)...
July 19, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28835462/a-novel-pyrin-associated-autoinflammation-with-neutrophilic-dermatosis-mutation-further-defines-14-3-3-binding-of-pyrin-and-distinction-to-familial-mediterranean-fever
#14
Fiona Moghaddas, Rafael Llamas, Dominic De Nardo, Helios Martinez-Banaclocha, Juan J Martinez-Garcia, Pablo Mesa-Del-Castillo, Paul J Baker, Vanessa Gargallo, Anna Mensa-Vilaro, Scott Canna, Ian P Wicks, Pablo Pelegrin, Juan I Arostegui, Seth L Masters
OBJECTIVE: Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis (PAAND) is a recently described monogenic autoinflammatory disease. The causal p.S242R MEFV mutation disrupts a binding motif of the regulatory 14-3-3 proteins within pyrin. Here, we investigate a family with clinical features consistent with PAAND in whom the novel p.E244K MEFV mutation, located in the +2 site of the 14-3-3 binding motif in pyrin, has been found. METHODS: Multiplex cytokine analyses were performed on p...
August 23, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28827415/antimicrobial-activity-of-ceftazidime-avibactam-tested-against-multidrug-resistant-enterobacteriaceae-and-pseudomonas-aeruginosa-isolates-from-united-states-medical-centers-2013-2016
#15
Helio S Sader, Mariana Castanheira, Dee Shortridge, Rodrigo E Mendes, Robert K Flamm
The in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 Enterobacteriaceae and 7,868 Pseudomonas aeruginosa. Isolates were consecutively collected from 94 US hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (JMI Laboratories). Enterobacteriaceae isolates resistant to carbapenems (CRE) and/or ceftazidime-avibactam (MIC ≥16 μg/mL) were evaluated for the presence of genes encoding ESBLs, KPC, NDM, and transferable AmpC enzymes...
August 21, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28818065/case-reports-of-two-pedigrees-with-recessive-arrhythmogenic-right-ventricular-cardiomyopathy-associated-with-homozygous-thr335ala-variant-in-dsg2
#16
Sami Qadri, Olli Anttonen, Juho Viikilä, Eija H Seppälä, Samuel Myllykangas, Tero-Pekka Alastalo, Miia Holmström, Tiina Heliö, Juha W Koskenvuo
BACKGROUND: Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited cardiac disease, involving changes in ventricular myocardial tissue and leading to fatal arrhythmias. Mutations in desmosomal genes are thought to be the main cause of ARVC. However, the exact molecular genetic etiology of the disease still remains largely inconclusive, and this along with large variabilities in clinical manifestations complicate clinical diagnostics. CASE PRESENTATION: We report two families (n = 20) in which a desmoglein-2 (DSG2) missense variant c...
August 17, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/28817229/performance-equivalency-between-computer-based-and-traditional-pen-and-paper-assessment-a-case-study-in-clinical-anatomy
#17
Bruno Guimarães, José Ribeiro, Bernardo Cruz, André Ferreira, Hélio Alves, Ricardo Cruz-Correia, Maria Dulce Madeira, Maria Amélia Ferreira
The time, material, and staff-consuming nature of anatomy's traditional pen-and-paper assessment system, the increase in the number of students enrolling in medical schools and the ever-escalating workload of academic staff have made the use of computer-based assessment (CBA) an attractive proposition. To understand the impact of such shift in the assessment method, an experimental study evaluating its effect on students' performance was designed. Additionally, students' opinions toward CBA were gathered. Second-year medical students attending a Clinical Anatomy course were randomized by clusters in two groups...
August 17, 2017: Anatomical Sciences Education
https://www.readbyqxmd.com/read/28813088/cognitive-dysfunction-in-corticobasal-degeneration
#18
Laís Machado de Oliveira, Igor Barcellos, Hélio A G Teive, Renato Puppi Munhoz
Corticobasal degeneration (CBD) was originally described as a distinct clinicopathological entity in 1967. Since then, different phenotypic presentations have emerged as possible manifestations of CBD histopathological findings. In addition, pathophysiological findings and the molecular basis have been delineated and several aspects of its cognitive manifestations have been clarified. Thus, not only the spectrum of what is currently designated as CBD has expanded, but overlap with other degenerative and even secondary disorders has made clinical diagnostic certainty even more challenging in the absence of specific and readily-available markers...
August 2017: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/28813078/quality-of-life-in-parkinson-s-disease-patients-progression-markers-of-mild-to-moderate-stages
#19
Raissa Carla Moreira, Marise Bueno Zonta, Ana Paula Serra de Araújo, Vera Lúcia Israel, Hélio A G Teive
Objective: To investigate which factors are associated with the quality of life decline in Parkinson's disease patients from mild to moderate stages. Methods: The Unified Parkinson's Disease Rating Scale and Parkinson's Disease Questionnaire-39 were used to evaluate clinical/functional data and the quality of life. Results: The markers of clinical/functional worsening were drooling (p < 0.004), need for assistance with hygiene (p = 0.02), greater freezing frequency (p = 0...
August 2017: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/28805497/putative-role-of-glutamine-in-the-activation-of-cbl-cipk-signalling-pathways-during-salt-stress-in-sorghum
#20
Rafael de Souza Miranda, Juan Carlos Alvarez-Pizarro, José Hélio Costa, Stelamaris de Oliveira Paula, José Tarquinio Prisco, Enéas Gomes-Filho
The salt overly sensitive (SOS) pathway is the only mechanism known for Na(+) extrusion in plant cells. SOS pathway activation involves Ca(2+)-sensing proteins, such as calcineurin B-like (CBL) proteins, and CBL-interacting protein kinases (CIPKs). In this signalling mechanism, a transit increase in cytosolic Ca(2+) concentration triggered by Na(+) accumulation is perceived by CBL (also known as SOS3). Afterward, SOS3 physically interacts with a CIPK (also known as SOS2), forming the SOS2/SOS3 complex, which can regulate the number downstream targets, controlling ionic homeostasis...
August 14, 2017: Plant Signaling & Behavior
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