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https://www.readbyqxmd.com/read/28345003/expansion-of-human-tregs-from-cryopreserved-umbilical-cord-blood-for-gmp-compliant-autologous-adoptive-cell-transfer-therapy
#1
Howard R Seay, Amy L Putnam, Judit Cserny, Amanda L Posgai, Emma H Rosenau, John R Wingard, Kate F Girard, Morey Kraus, Angela P Lares, Heather L Brown, Katherine S Brown, Kristi T Balavage, Leeana D Peters, Ashley N Bushdorf, Mark A Atkinson, Jeffrey A Bluestone, Michael J Haller, Todd M Brusko
Umbilical cord blood is a traditional and convenient source of cells for hematopoietic stem cell transplantation. Thymic regulatory T cells (Tregs) are also present in cord blood, and there is growing interest in the use of autologous Tregs to provide a low-risk, fully human leukocyte antigen (HLA)-matched cell product for treating autoimmune diseases, such as type 1 diabetes. Here, we describe a good manufacturing practice (GMP)-compatible Treg expansion protocol using fluorescence-activated cell sorting, resulting in a mean 2,092-fold expansion of Tregs over a 16-day culture for a median yield of 1...
March 17, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28342611/short-communication-identification-of-corynebacterium-bovis-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#2
Helio Langoni, Carolina Polo Camargo da Silva, Marcella Zampoli Troncarelli, Alessandra Tata, Katia Roberta Anacleto Belaz, Marcos Nogueira Eberlin, Samea Fernandes Joaquim, Felipe Freitas Guimarães, Renata Bonini Pardo, Eduardo Nardini Gomes
Corynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28333332/wck-5222-cefepime-zidebactam-antimicrobial-activity-tested-against-gram-negative-organisms-producing-clinically-relevant-%C3%AE-lactamases
#3
Helio S Sader, Paul R Rhomberg, Robert K Flamm, Ronald N Jones, Mariana Castanheira
Background: Zidebactam is a β-lactam enhancer antibiotic with a dual mechanism of action involving binding to Gram-negative PBP2 and β-lactamase inhibition. Cefepime combined with zidebactam (WCK 5222) is under clinical development for treatment of Gram-negative infections. Objectives: To evaluate the in vitro activities of cefepime and zidebactam separately and combined at 1:1 and 2:1 ratios when tested against Gram-negative organisms producing the most clinically relevant β-lactamase types...
March 18, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28328211/the-water-solvent-effect-on-theoretical-evaluation-of-1h-nmr-chemical-shifts-the-o-methyl-inositol-isomer
#4
Helio F Dos Santos, Marcelo Chagas, Leonardo De Souza, Willian Rocha, Mauro V De Almeida, Cleber P A Anconi, Wagner Batista De Almeida
In this paper, density functional theory calculations of nuclear magnetic resonance (NMR) chemical shifts for L-quebrachitol isomer, previously studied in our group (Mag. Res. Chem. 50 (2012) 608-614) are reported aiming to investigate in more details the water solvent effect on the prediction of 1H NMR spectra. In order to include explicit water molecules, twenty water-L-quebrachitol configurations obtained from Monte Carlo simulation were selected to perform geometry optimizations using the effective fragment potential method encompassing 60 water molecules around the solute...
March 22, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28326135/regiochemistry-of-cyclocondensation-reactions-in-the-synthesis-of-polyazaheterocycles
#5
Patrick T Campos, Leticia V Rodrigues, Andrei L Belladona, Caroline R Bender, Juliana S Bitencurt, Fernanda A Rosa, Davi F Back, Helio G Bonacorso, Nilo Zanatta, Clarissa P Frizzo, Marcos A P Martins
The syntheses of several polyazaheterocycles are demonstrated. The cyclocondensation reactions between β-enaminodiketones [CCl3C(O)C(=CNMe2)C(O)-CO2Et] and aromatic amidines resulted in glyoxalate-substituted pyrido[1,2-a]pyrimidinone, thiazolo[3,2-a]pyrimidinone and pyrimido[1,2-a]benzimidazole. Pyrazinones and quinoxalinones were obtained through the reaction of these glyoxalates with ethylenediamine and 1,2-phenylenediamine derivatives. On the other hand, the reaction of glyoxalates with amidines did not lead to the formation of imidazolones, but rather N-acylated products were obtained...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28325447/statistical-tools-for-the-temporal-analysis-and-classification-of-lung-lesions
#6
Stelmo Magalhães Barros Netto, Aristófanes Corrêa Silva, Hélio Lopes, Anselmo Cardoso de Paiva, Rodolfo Acatauassú Nunes, Marcelo Gattass
BACKGROUND AND OBJECTIVE: Lung cancer remains one of the most common cancers globally. Temporal evaluation is an important tool for analyzing the malignant behavior of lesions during treatment, or of indeterminate lesions that may be benign. This work proposes a methodology for the analysis, quantification, and visualization of small (local) and large (global) changes in lung lesions. In addition, we extract textural features for the classification of lesions as benign or malignant. METHODS: We employ the statistical concept of uncertainty to associate each voxel of a lesion to a probability that changes occur in the lesion over time...
April 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28316600/methylation-of-foxp3-tsdr-underlies-the-impaired-suppressive-function-of-tregs-from-long-term-belatacept-treated-kidney-transplant-patients
#7
Evelyn Katy Alvarez Salazar, Arimelek Cortés-Hernández, Germán Rodrigo Alemán-Muench, Josefina Alberú, Jesús R Rodríguez-Aguilera, Félix Recillas-Targa, Victoria Chagoya de Sánchez, Eric Cuevas, Eduardo Mancilla-Urrea, María Pérez García, Guillermo Mondragón-Ramírez, Mario Vilatobá, Ian Bostock, Erick Hernández-Méndez, David De Rungs, Eduardo A García-Zepeda, Gloria Soldevila
Regulatory T cells (Tregs) are considered key players in the prevention of allograft rejection in transplanted patients. Belatacept (BLT) is an effective alternative to calcineurin inhibitors that appears to preserve graft survival and function; however, the impact of this drug in the homeostasis of Tregs in transplanted patients remains controversial. Here, we analyzed the phenotype, function, and the epigenetic status of the Treg-specific demethylated region (TSDR) in FOXP3 of circulating Tregs from long-term kidney transplant patients under BLT or Cyclosporine A treatment...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28316087/influence-of-abcc2-cyp2c8-and-cyp2j2-polymorphisms-on-tacrolimus-and-mycophenolate-sodium-based-treatment-in-brazilian-kidney-transplant-recipients
#8
Fabiana D V Genvigir, Alvaro M Nishikawa, Claudia R Felipe, Helio Tedesco-Silva, Nagilla Oliveira, Antony B C Salazar, Jose O Medina-Pestana, Sonia Q Doi, Mario H Hirata, Rosario D C Hirata
STUDY OBJECTIVE: To investigate the influence of single nucleotide polymorphisms (SNPs) in genes encoding metabolizing enzymes (CYP2C8, CYP2J2, and UGT2B7) and transporters (ABCC2 and ABCG2) on dose and/or dose-adjusted trough blood concentrations (C/D ratio), clinical outcomes, and occurrence of adverse events of tacrolimus and mycophenolate sodium in Brazilian kidney transplant recipients. DESIGN: Pharmacogenetic analysis of patients enrolled in a previously published study...
March 17, 2017: Pharmacotherapy
https://www.readbyqxmd.com/read/28304401/a-critical-appraisal-of-the-morphological-criteria-for-diagnosing-intestinal-neuronal-dysplasia-type-b
#9
Simone A Terra, Pedro L de Arruda Lourenção, Márcia G Silva, Hélio A Miot, Maria A M Rodrigues
Intestinal neuronal dysplasia type B is a controversial entity expressed by complex changes in the enteric nervous system. Diagnosis depends on rectal biopsy histopathology and diagnostic criteria, both qualitative and quantitative, have changed over time, hindering the diagnostic practice. We analyzed the morphological criteria for the histological diagnosis of intestinal neuronal dysplasia type B in a series of patients with intestinal neuronal dysplasia type B according to the 1990 Frankfurt Consensus criteria and verified the applicability of the numerical criteria proposed by Meier-Ruge et al in 2004 and 2006...
March 17, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28301970/disclosing-the-parameters-leading-to-high-productivity-of-retroviral-producer-cells-lines-evaluating-random-vs-targeted-integration
#10
Vanessa Sofia Bandeira, Hélio A Tomás, Evren Alici, Manuel J C T Carrondo, Ana Sofia Coroadinha
Gammaretrovirus and lentivirus are the preferred viral vectors to genetically modify T- and NK- cells to be used in immune-cell therapies. The transduction efficiency of hematopoietic and T cells is more efficient using Gibbon ape leukemia virus (GaLV) pseudotyping. In this context gammaretroviral vector producer cells offer competitive higher titers than transient lentiviral vectors productions. The main aim of this work was to identify the key parameters governing GalV pseudotyped gammaretroviral vector productivity in stable producer cells using a retroviral vector expression cassette enabling positive (facilitating cell enrichment) and negative cell selection (allowing cell elimination)...
March 16, 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/28296931/helios-expression-and-foxp3-tsdr-methylation-of-ifny-and-ifny-treg-from-kidney-transplant-recipients-with-good-long-term-graft-function
#11
Karina Trojan, Christian Unterrainer, Rolf Weimer, Nuray Bulut, Christian Morath, Mostafa Aly, Li Zhu, Gerhard Opelz, Volker Daniel
BACKGROUND: There is circumstantial evidence that IFNy+ Treg might have clinical relevance in transplantation. IFNy+ Treg express IFNy receptors and are induced by IFNy. In the present study we investigated in kidney transplant recipients with good long-term stable graft function the absolute cell counts of IFNy+ Treg subsets and whether their expression of Foxp3 is stable or transient. METHOD: Helios expression determined by eight-color-fluorescence flow cytometry and methylation status of the Foxp3 Treg specific demethylation region (TSDR) served as indicators for stability of Foxp3 expression...
2017: PloS One
https://www.readbyqxmd.com/read/28292653/co-circulating-serotypes-in-a-dengue-fever-outbreak-differential-hematological-profiles-and-phylogenetic-relationships-among-viruses
#12
Andreia Moreira Dos Santos Carmo, Rodrigo Buzinaro Suzuki, Aline Diniz Cabral, Renata Torres da Costa, Gabriela Pena Massari, Michele Marcondes Riquena, Helio Augusto Alves Fracasso, Andre Eterovic, Arlei Marcili, Márcia Aparecida Sperança
BACKGROUND: Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília. OBJECTIVES: The aim of this article was to distinguish dengue serotypes circulating during the 2007 Marília outbreak and define their association to demographic and hematological patient profiles, as well as the phylogenetic relationships among the different viruses...
March 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28291341/structural-electronic-and-thermodynamic-properties-of-the-t-and-b-phases-of-niobia-first-principle-calculations
#13
Mirele Bastos Pinto, Antonio Lenito Soares, Andy Mella Orellana, Helio Anderson Duarte, Heitor Avelino de Abreu
Different polymorphs of the Nb2O5 can be obtained depending on the pressure and temperature of calcination leading to different catalytic properties. Two polymorphs of niobia, T-Nb2O5 and B-Nb2O5 have been investigated by means of density funtional/plane waves method. The equation of state predicted that B-Nb2O5 phase is more stable than the T-Nb2O5 at low temperature and at high pressure both phases are stable in a good agreement with the available experimental data. The calculated cohesive energies of 8.56 and 8...
March 14, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28291066/quality-of-life-with-ingrown-toenails-a-cross-sectional-study
#14
Alessandra de Pinho Pimenta Borges, Valquíria Pessoa Chinem Pelafsky, Luciane Donida Bartoli Miot, Hélio Amante Miot
No abstract text is available yet for this article.
March 13, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28289129/helios-expression-coordinates-the-development-of-a-subset-of-striatopallidal-medium-spiny-neurons
#15
Raquel Martín-Ibáñez, Mónica Pardo, Albert Giralt, Andrés Miguez, Inés Guardia, Lucile Marion-Poll, Cristina Herranz, Miriam Esgleas, Gerardo Garcia-Díaz Barriga, Michael J Edel, Carlos Vicario-Abejón, Jordi Alberch, Jean-Antoine Girault, Susan Chan, Philippe Kastner, Josep M Canals
Here we unravel the mechanism of action of Helios (He) during the development of striatal medium spiny neurons (MSNs). He regulates the second wave of striatal neurogenesis involved in the generation of striatopallidal neurons that express dopamine 2 receptor (D2R) and enkephalin (ENK). To exert this effect He is expressed in neural progenitor cells (NPCs) retaining them into the G1/G0 phase of the cell cycle. Thus, the lack of He produces an increase of S-phase entry and S-phase length of NPCs which in turn impairs striatal neurogenesis and produces an accumulation of the number of cycling NPCs in the germinal zone (GZ) that end up dying at postnatal stages...
March 13, 2017: Development
https://www.readbyqxmd.com/read/28286113/cd4-foxp3-t-regulatory-cell-subsets-in-myasthenia-gravis-patients
#16
Siegfried Kohler, Thomas Oskar Philipp Keil, Sarah Hoffmann, Marc Swierzy, Mahmoud Ismail, Jens Carsten Rückert, Tobias Alexander, Andreas Meisel
Although myasthenia gravis (MG) is a classic autoantibody-mediated disease, T cells are centrally involved in its pathogenesis. In recent years a number of studies have analyzed the role of CD4(+) FoxP3(+) regulatory T cells (Treg) in the disease with contradictory results. Here, the generation of Treg was significantly reduced in thymoma as compared to thymic hyperplasia and normal thymus tissue (p=0.0002). In the peripheral blood, Treg subsets classified according to CD49d, HELIOS and CD45RA expression changed after thymectomy and in the long-term course of immunosuppression...
March 9, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28284938/human-il-6r-hi-tigit-cd4-cd127-low-cd25-t-cells-display-potent-in-vitro-suppressive-capacity-and-a-distinct-th17-profile
#17
Ricardo C Ferreira, Daniel B Rainbow, Arcadio Rubio García, Marcin L Pekalski, Linsey Porter, João J Oliveira, Frank Waldron-Lynch, Linda S Wicker, John A Todd
To date many clinical studies aim to increase the number and/or fitness of CD4(+)CD127(low)CD25(+) regulatory T cells (Tregs) in vivo to harness their regulatory potential in the context of treating autoimmune disease. Here, we sought to define the phenotype and function of Tregs expressing the highest levels of IL-6 receptor (IL-6R). We have identified a population of CD4(+)CD127(low)CD25(+) TIGIT(-) T cells distinguished by their elevated IL-6R expression that lacked expression of HELIOS, showed higher CTLA-4 expression, and displayed increased suppressive capacity compared to IL-6R(hi)TIGIT(+) Tregs...
March 9, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28283778/plk1-associated-micrornas-are-correlated-with-pediatric-medulloblastoma-prognosis
#18
Julia Alejandra Pezuk, María Sol Brassesco, Ricardo Santos de Oliveira, Hélio Rubens Machado, Luciano Neder, Carlos Alberto Scrideli, Luiz Gonzaga Tone
PURPOSE: Medulloblastoma (MB) is the most common malignant tumor of the central nervous system (CNS) in children. Despite its relative good survival rates, treatment can cause long time sequels and may impair patients' lifespan and quality, making the search for new treatment options still necessary. Polo like kinases (PLKs) constitute a five-member serine/threonine kinases family (PLK 1-5) that regulates different stages during cell cycle. Abnormal PLKs expression has been observed in several cancer types, including MB...
March 10, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28278442/exogenous-nitric-oxide-improves-salt-tolerance-during-establishment-of-jatropha-curcas-seedlings-by-ameliorating-oxidative-damage-and-toxic-ion-accumulation
#19
Cibelle Gomes Gadelha, Rafael de Souza Miranda, Nara Lídia M Alencar, José Hélio Costa, José Tarquinio Prisco, Enéas Gomes-Filho
Jatropha curcas is an oilseed species that is considered an excellent alternative energy source for fossil-based fuels for growing in arid and semiarid regions, where salinity is becoming a stringent problem to crop production. Our working hypothesis was that nitric oxide (NO) priming enhances salt tolerance of J. curcas during early seedling development. Under NaCl stress, seedlings arising from NO-treated seeds showed lower accumulation of Na(+) and Cl(-) than those salinized seedlings only, which was consistent with a better growth for all analyzed time points...
February 20, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28278428/effect-of-lead-on-physiological-and-antioxidant-responses-in-two-vigna-unguiculata-cultivars-differing-in-pb-accumulation
#20
Nila Maria Bezerril Fontenele, Maria de Lourdes Oliveira Otoch, Neuza Félix Gomes-Rochette, Alana Cecília de Menezes Sobreira, Adolph Annderson Gonçalves Costa Barreto, Francisco Dalton Barreto de Oliveira, José Hélio Costa, Simone da Silveira Sá Borges, Ronaldo Ferreira do Nascimento, Dirce Fernandes de Melo
Lead (Pb) is one of the most toxic anthropogenic pollutants, occurring widely in both terrestrial and aquatic ecosystems, where it impairs plant growth and development. In this work, the effect of 0.5 mM EDTA-Pb was evaluated in two Vigna unguiculata cultivars (SV and SET), with the aim of detecting genotype/cultivar dependent changes in the physiological and anti-oxidant responses (CAT and APX) of a leguminous plant. The data showed that SV accumulated more Pb in roots while SET accumulated more in leaves, indicating differential regulation in Pb-translocation/accumulation...
February 17, 2017: Chemosphere
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