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https://www.readbyqxmd.com/read/29353442/flexible-pseudotyping-of-retrovirus-using-recombinase-mediated-cassette-exchange
#1
Hugo R Soares, Ana I Almeida, Hélio A Tomás, Paula M Alves, Ana S Coroadinha
OBJECTIVE: Develop an engineered cell line containing two flexible gene expression systems enabling the continuous production of tailor-made recombinant gammaretrovirus with predictable productivities through targeted integration. RESULTS: Dual-FLEX cells (dFLEX) contain two independent recombinase-mediated cassette exchange (RMCE) systems which confer flexibility to the expression of different transgene and envelope combinations. The flexible envelope expression in dFLEX cells was validated by pseudotyping retrovirus particles with three different viral envelope proteins-GaLV, 4070A and VSV-G...
January 20, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29352562/cbs-mutations-are-good-predictors-for-b6-responsiveness-a-study-based-on-the-analysis-of-35-brazilian-classical-homocystinuria-patients
#2
Soraia Poloni, Fernanda Sperb-Ludwig, Taciane Borsatto, Giovana Weber Hoss, Maria Juliana R Doriqui, Emília K Embiruçu, Ney Boa-Sorte, Charles Marques, Chong A Kim, Carolina Fischinger Moura de Souza, Helio Rocha, Marcia Ribeiro, Carlos E Steiner, Carolina A Moreno, Pricila Bernardi, Eugenia Valadares, Osvaldo Artigalas, Gerson Carvalho, Hector Y C Wanderley, Johanna Kugele, Melanie Walter, Lorena Gallego-Villar, Henk J Blom, Ida Vanessa D Schwartz
BACKGROUND: Classical homocystinuria (HCU) is a monogenic disease caused by the deficient activity of cystathionine β-synthase (CβS). The objective of this study was to identify the CBS mutations in Brazilian patients with HCU. METHODS: gDNA samples were obtained for 35 patients (30 families) with biochemically confirmed diagnosis of HCU. All exons and exon-intron boundaries of CBS gene were sequenced. Gene expression analysis by qRT-PCR was performed in six patients...
January 20, 2018: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29340780/health-related-quality-of-life-in-outpatients-with-schizophrenia-factors-that-determine-changes-over-time
#3
Cristina Domenech, Carlo Altamura, Corrado Bernasconi, Ricardo Corral, Helio Elkis, Jonathan Evans, Ashok Malla, Marie-Odile Krebs, Anna-Lena Nordstroem, Mathias Zink, Josep Maria Haro
PURPOSE: The objective of this study was to analyze the clinical factors associated with changes in HRQoL in outpatients with schizophrenia using both generic and condition-specific HRQoL scales. METHODS: Adult outpatients with schizophrenia at least 18 years of age who did not have an acute psychotic exacerbation in the 3 months prior to baseline were recruited. PANSS dimensions were calculated based on Lindenmayer et al.'s five factors. HRQoL data were assessed by patients using the Schizophrenia Quality of Life Scale (SQLS), the Short Form-36 (SF-36), and the EuroQol-5 Dimension (EQ-5D) questionnaires...
January 16, 2018: Social Psychiatry and Psychiatric Epidemiology
https://www.readbyqxmd.com/read/29340468/serosurvey-of-leptospira-spp-and-toxoplasma-gondii-in-rats-captured-from-two-zoos-in-southern-brazil
#4
Maysa Pellizzaro, Francisco de Oliveira Conrado, Camila Marinelli Martins, Sâmea Fernandes Joaquim, Fernando Ferreira, Helio Langoni, Alexander Welker Biondo
INTRODUCTION: Norway rats (Rattus norvegicus) are zoonotic reservoirs for Leptospira spp. and Toxoplasma gondii, and influence diseases in urban areas. METHODS: Free-ranging and laboratory-raised rats from two zoos in southern Brazil were tested for Leptospira spp. and T. gondii using microscopic agglutination and modified agglutination tests, respectively. RESULTS: Overall, 25.6% and 4.6% free-ranging rats tested positive for Leptospira spp...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29339969/a-new-toolset-for-protein-expression-and-subcellular-localization-studies-in-citrus-and-its-application-to-citrus-tristeza-virus-proteins
#5
Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin, Vladimir Orbovic
Background: Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium...
2018: Plant Methods
https://www.readbyqxmd.com/read/29330014/frequency-of-red-blood-cell-genotypes-in-multi-transfused-patients-and-blood-donors-from-minas-gerais-southeast-brazil
#6
Vitor Mendonça Alves, Fernanda Bernadelli De Vito, Paulo Roberto Juliano Martins, Sheila Soares Silva, Lilian Castilho, Helio Moraes-Souza
BACKGROUND AND OBJECTIVES: The frequency of red blood cell (RBC) antigens in Brazil varies due to differences in the ethnic groups in different regions; however, these studies have not been performed in Minas Gerais, where African admixture is more prevalent in comparison with other states. Due to these facts, this study aimed to determine the frequency of RBC genotypes on Rh, Kell, Duffy and Kidd systems in blood donors and multi-transfused patients from Minas Gerais, Southeast Brazil...
December 16, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29324790/frog-size-on-continental-islands-of-the-coast-of-rio-de-janeiro-and-the-generality-of-the-island-rule
#7
Raoni Rebouças, Hélio Ricardo da Silva, Mirco Solé
Island Rule postulated that individuals on islands tend to dwarfism when individuals from mainland populations are large and to gigantism when mainland populations present small individuals. There has been much discussion about this rule, but only few studies were carried out aiming to reveal this pattern for anurans. Our study focused on measuring the size of individuals on islands and to find a possible pattern of size modification for insular anurans. Individuals were collected on continental islands, measured and compared to mainland populations...
2018: PloS One
https://www.readbyqxmd.com/read/29319770/factors-associated-with-hospital-mortality-in-renal-transplant-patients-admitted-to-the-intensive-care-unit-with-acute-respiratory-failure
#8
Rafael Mendes da Silva, Flavio Geraldo Rezende de Freitas, Antonio Tonete Bafi, Hélio Tedesco Silva Junior, Bartira de Aguiar Roza
INTRODUCTION: The lungs are often involved in a variety of complications after kidney transplantation. Acute respiratory failure (ARF) is one of the most serious manifestations of pulmonary involvement. OBJECTIVE: To describe the main causes of ARF in kidney transplant patients who require intensive care and identify the factors associated with mortality. METHODS: This retrospective study evaluated adult patients with ARF admitted to the intensive care unit of a center with high volume of transplants from August 2013 to August 2015...
October 2017: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/29319768/the-current-burden-of-cytomegalovirus-infection-in-kidney-transplant-recipients-receiving-no-pharmacological-prophylaxis
#9
Claudia Rosso Felipe, Alexandra Nicolau Ferreira, Adrieli Bessa, Tamiris Abait, Priscilla Ruppel, Mayara Ivani de Paula, Liliane Hiramoto, Laila Viana, Suelen Martins, Marina Cristelli, Wilson Aguiar, Juliana Mansur, Geovana Basso, Helio Tedesco Silva Junior, Jose Medina Pestana
Cytomegalovirus (CMV) infection in kidney transplantation has changed its clinical spectrum, mostly due to the current and more effective immunosuppression. In the absence of preventive strategies it is associated with significant morbi-mortality. OBJECTIVE: This study evaluated the incidence of CMV events and its effect on outcomes of kidney transplantation in recipients without pharmacological prophylaxis or targeted preemptive treatment. RESULTS: The study cohort comprised 802 recipients of kidney transplants between 04/30/2014 and 04/30/2015...
October 2017: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/29317289/baroreflex-stimulation-attenuates-central-but-not-peripheral-inflammation-in-conscious-endotoxemic-rats
#10
Fernanda Brognara, Jaci A Castania, Daniel P M Dias, Alexandre H Lopes, Rubens Fazan, Alexandre Kanashiro, Luis Ulloa, Helio C Salgado
We previously reported that activation of the baroreflex, a critical physiological mechanism controlling cardiovascular homeostasis, through electrical stimulation of the aortic depressor nerve attenuates joint inflammation in experimental arthritis. However, it is unknown whether baroreflex activation can control systemic inflammation. Here, we investigate whether baroreflex activation controls systemic inflammation in conscious endotoxemic rats. Animals underwent sham or electrical aortic depressor nerve stimulation initiated 10 min prior to a lipopolysaccharide (LPS) challenge, while inflammatory cytokine levels were measured in the blood, spleen, heart and hypothalamus 90 min after LPS treatment...
January 6, 2018: Brain Research
https://www.readbyqxmd.com/read/29313987/variable-role-of-carotid-bodies-in-cardiovascular-responses-to-exercise-hypoxia-and-hypercapnia-in-spontaneously-hypertensive-rats
#11
Wioletta Pijacka, Pedro L Katayama, Helio C Salgado, Gisele S Lincevicius, Ruy R Campos, Fiona D McBryde, Julian F R Paton
The carotid body has recently emerged as a promising therapeutic target for treating cardiovascular disease, however the potential impact of carotid bodies removal on the dynamic cardiovascular responses to acute stressors such as exercise, hypoxia and hypercapnia in hypertension is an important safety consideration that has not been studied. We first validated a novel surgical approach to selectively resect the carotid bodies bilaterally (CBR) sparing the carotid sinus baroreflex. Second, we evaluated the impact of CBR on the cardiovascular responses to exercise, hypoxia and hypercapnia in the conscious, chronically instrumented spontaneously hypertensive (SH) rats...
January 5, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29300171/universal-deformation-pathways-and-flexural-hardening-of-nanoscale-2d-material-standing-folds
#12
Helio Chacham, Ana Barboza, Alan B de Oliveira, Camilla Oliveira, Ronaldo Batista, Bernardo Ruegger Almeida Neves
In the present work, we use atomic force microscopy nanomanipulation of 2D-material standing folds to investigate their mechanical deformation. Using graphene, h-BN and talc nanoscale wrinkles as testbeds, universal force-strain pathways are clearly uncovered and well-accounted for by an analytical model. Such universality further enables the investigation of each fold bending stiffness κ as a function of its characteristic height h0. We observe a more than tenfold increase of κ as h0 increases in the 10-100 nm range, with power-law behaviors of κ versus h0 with exponents larger than unity for the three materials...
January 4, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29299806/role-of-%C3%AE-fetoprotein-in-differentiation-of-regulatory-t-lymphocytes
#13
V A Chereshnev, V P Timganova, S A Zamorina, M S Bochkova, P V Khramtsov, M D Kropaneva, M B Raev
The effect of native α-fetoprotein (AFP) on the expression of T-regulatory lymphocyte (Treg) markers by activated CD4+ lymphocytes with different proliferative status was studied. α-Fetoprotein did not affect the ratio of proliferating and non-proliferating activated CD4+ cells. In the study of Treg differentiation, it was found that AFP at concentrations of 50 and 100 μg/mL significantly inhibited the number of nonproliferating CD4+FOXP3+ and CD4+FOXP3+HELIOS+ lymphocytes without affecting the expression of Treg markers by proliferating CD4+ lymphocytes...
November 2017: Doklady Biological Sciences: Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
https://www.readbyqxmd.com/read/29295653/improvement-of-fatigue-physical-functioning-and-well-being-among-patients-with-severe-impairment-at-baseline-receiving-ibrutinib-in-combination-with-bendamustine-and-rituximab-for-relapsed-chronic-lymphocytic-leukemia-small-lymphocytic-lymphoma-in-the-helios
#14
Paula Cramer, Graeme Fraser, Rodrigo Santucci-Silva, Sebastian Grosicki, Marie-Sarah Dilhuydy, Ann Janssens, Javier Loscertales, Simon Rule, Andre Goy, Shana Traina, Eric K H Chan, Joris Diels, Nishan Sengupta, Michelle Mahler, Mariya Salman, Angela Howes, Asher Chanan-Khan
Health-related quality of life (HRQoL) is an important endpoint, especially in clinical trials for malignancies with a long course of disease, such as chronic lymphocytic leukemia (CLL). Patient-reported outcomes were examined in the randomized, double-blind, placebo-controlled HELIOS study to assess the impact of treatment with the Bruton's tyrosine kinase inhibitor ibrutinib, added to bendamustine plus rituximab (BR) background therapy. Measures included FACIT-Fatigue, EORTC QLQ-C30, QLQ-CLL16, and EQ-5D-5L...
January 3, 2018: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29290031/specialized-plant-metabolism-characteristics-and-impact-on-target-molecule-biotechnological-production
#15
REVIEW
Hélio Nitta Matsuura, Sonia Malik, Fernanda de Costa, Morteza Yousefzadi, Mohammad Hossein Mirjalili, Randolph Arroo, Avninder S Bhambra, Miroslav Strnad, Mercedes Bonfill, Arthur Germano Fett-Neto
Plant secondary metabolism evolved in the context of highly organized and differentiated cells and tissues, featuring massive chemical complexity operating under tight environmental, developmental and genetic control. Biotechnological demand for natural products has been continuously increasing because of their significant value and new applications, mainly as pharmaceuticals. Aseptic production systems of plant secondary metabolites have improved considerably, constituting an attractive tool for increased, stable and large-scale supply of valuable molecules...
December 30, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29289349/motor-units-in-vastus-lateralis-and-in-different-vastus-medialis-regions-show-different-firing-properties-during-low-level-isometric-knee-extension-contraction
#16
Leonardo Mendes Leal de Souza, Hélio Veiga Cabral, Liliam Fernandes de Oliveira, Taian Martins Vieira
Architectural differences along vastus medialis (VM) and between VM and vastus lateralis (VL) are considered functionally important for the patellar tracking, knee joint stability and knee joint extension. Whether these functional differences are associated with a differential activity of motor units between VM and VL is however unknown. In the present study, we, therefore, investigate neuroanatomical differences in the activity of motor units detected proximo-distally from VM and from the VL muscle. Nine healthy volunteers performed low-level isometric knee extension contractions (20% of their maximum voluntary contraction) following a trapezoidal trajectory...
December 28, 2017: Human Movement Science
https://www.readbyqxmd.com/read/29278858/the-ikaros-family-in-lymphocyte-development
#17
REVIEW
Beate Heizmann, Philippe Kastner, Susan Chan
The IKZF family of transcription factors are essential regulators of lymphopoiesis. Ikaros, Helios, Aiolos and Eos function as transcriptional repressors and activators during T and B cell differentiation and in mature cell function, depending on the stage of development and/or cell type. Their potential mechanisms of action are varied. Ikaros family proteins partner with multiple complexes, including NuRD, PRC2 and transcription elongation factors, to modulate gene expression and the chromatin state. In humans, mutations in the IKZF genes are associated with B cell deficiency, leukemias and autoimmunity...
December 23, 2017: Current Opinion in Immunology
https://www.readbyqxmd.com/read/29277526/dalbavancin-in-vitro-activity-obtained-against-gram-positive-clinical-isolates-causing-bone-and-joint-infections-in-united-states-and-european-hospitals-2011-2016
#18
Michael A Pfaller, Robert K Flamm, Mariana Castanheira, Helio S Sader, Rodrigo E Mendes
BACKGROUND: Osteomyelitis represents hard-to-treat infections that regularly involve prolonged systemic antibiotic use. Dalbavancin has demonstrated activity against gram-positive isolates and has been considered as a candidate for osteomyelitis therapy in adults and children. This study evaluates the activity of dalbavancin against pathogens isolated from bone and joint infections (BJI). METHODS: 801 S. aureus, 160 coagulase-negative staphylococci (CoNS), 164 β-haemolytic streptococci (BHS), 82 E...
December 22, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29259350/decreased-helios-expression-in-regulatory-t-cells-in-acute-coronary-syndrome
#19
Lili Jiang, Feng Chen, Xiaofan Hu, Yingying Hu, Yange Wang, Wenyong Zhang, Yudong Peng, Longxian Cheng
Regulatory T cells (Tregs) play an essential role in acute coronary syndrome (ACS). However, there is debate about which Treg subsets are truly critical to ACS. Helios, a transcription factor, was recently reported to be a bona fide marker for natural Tregs or activated Tregs with a suppression function, but little is known about its role in ACS. We therefore examined Helios+ Tregs in patients with ACS, patients with stable angina, and control subjects. 73 patients with ACS, 30 patients with stable angina, and 48 control subjects were enrolled...
2017: Disease Markers
https://www.readbyqxmd.com/read/29258377/clinical-features-of-kidney-transplant-recipients-admitted-to-the-intensive-care-unit
#20
Flávio Geraldo Rezende Freitas, Fábio Lombardi, Eduardo Souza Pacheco, Tainá Veras de Sandes-Freitas, Laila Almeida Viana, Hélio Tedesco-Silva Junior, José Osmar Medina-Pestana, Antônio Tonete Bafi, Flavia Ribeiro Machado
INTRODUCTION: There is a paucity of data regarding the complications in kidney transplant patients who may require intensive care unit (ICU) management, despite being the most common solid organ transplant worldwide. OBJECTIVE: To identify the main reasons for ICU admission and to determine the factors associated with hospital mortality in kidney transplant recipients. DESIGN: This single-center retrospective cohort study was conducted between September 2013 and June 2014, including all consecutive kidney transplant patients requiring ICU admission...
January 1, 2017: Progress in Transplantation
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