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https://www.readbyqxmd.com/read/28646266/pericytes-promote-abnormal-tumor-angiogenesis-in-a-rat-rg2-glioma-model
#1
Junji Hosono, Shunichi Morikawa, Taichi Ezaki, Takakazu Kawamata, Yoshikazu Okada
In glioma angiogenesis, tumor vessels cause morphological and functional abnormalities associated with malignancy and tumor progression. We hypothesized that certain structural changes or scantiness of functional pericytes may be involved in the formation of dysfunctional blood vessels in gliomas. In this study, we performed morphological examinations to elucidate the possible involvement of pericytes in brain tumor vessel abnormalities using a rat RG2 glioma model. After implantation of RG2 glioma cells in the syngeneic rat brain, gliomas were formed as early as day 7...
June 23, 2017: Brain Tumor Pathology
https://www.readbyqxmd.com/read/28646165/selective-inhibition-of-sterolo-acyltransferase-1-isozyme-by-beauveriolide-iii-in-intact-cells
#2
Taichi Ohshiro, Keisuke Kobayashi, Mio Ohba, Daisuke Matsuda, Lawrence L Rudel, Takashi Takahashi, Takayuki Doi, Hiroshi Tomoda
Beauveriolide III (BeauIII) inhibited sterol O-acyltransferases 1 and 2 (SOAT1 and SOAT2), which are endoplasmic reticulum (ER) membrane proteins, in an enzyme-based assay, and selectively inhibited SOAT1 in a cell-based assay using SOAT1-/SOAT2-CHO cells. This discrepancy in SOAT inhibition by BeauIII was investigated. In the enzyme-based assay, BeauIII inhibited SOAT1 and SOAT2 to a similar extent using microsomes prepared from cells disrupted under the strongest sonication condition. In semi-intact SOAT1-/SOAT2-CHO cells prepared by a treatment with digitonin (plasma membrane permeabilized), BeauIII selectively inhibited SOAT1 (IC50; 5...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28643250/genome-editing-in-mouse-zygotes-and-embryonic-stem-cells-by-introducing-sgrna-cas9-expressing-plasmids
#3
Taichi Noda, Asami Oji, Masahito Ikawa
In mammalian cells, genome editing with the single guide RNA (sgRNA)/Cas9 complex allows for high targeting efficiency within a relatively short time frame with the added benefits of being low cost and easy to design. sgRNA/Cas9-mediated editing in mouse zygotes has accelerated the analysis of gene functions and the generation of mouse models of human diseases. Despite the benefits, this method still suffers from several problems, such as mosaicism in the founder generation which complicates genotyping and phenotypical analyses, and the low efficiency of more complicated genome editing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28642169/a-novel-method-to-quantify-base-substitution-mutations-at-the-10-6-per-bp-level-in-dna-samples
#4
Satoshi Yamashita, Naoko Iida, Hideyuki Takeshima, Naoko Hattori, Maeda Masahiro, Takayoshi Kishino, Reiko Nagano, Taichi Shimazu, Shoichiro Tsugane, Toshikazu Ushijima
Somatic base substitution mutations of frequencies at the 10(-6)/bp level are expected to be present in many biomedical samples, such as tissues exposed to carcinogenic factors and exhausted stem cells. However, measurement of such rare mutations has been very difficult in human DNA samples. Here, we invented the use of 100 copies of genomic DNA as a template for amplicon deep sequencing so that a real mutation in a single DNA molecule would be detected at a variant allele frequency of 1% while sequencing errors have less frequency...
June 19, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28637947/neurosurgical-virtual-reality-simulation-for-brain-tumor-using-high-definition-computer-graphics-a-review-of-the-literature
#5
Taichi Kin, Hirofumi Nakatomi, Naoyuki Shono, Seiji Nomura, Toki Saito, Hiroshi Oyama, Nobuhito Saito
Simulation and planning of surgery using a virtual reality model is becoming common with advances in computer technology. In this study, we conducted a literature search to find trends in virtual simulation of surgery for brain tumors. A MEDLINE search for "neurosurgery AND (simulation OR virtual reality)" retrieved a total of 1,298 articles published in the past 10 years. After eliminating studies designed solely for education and training purposes, 28 articles about the clinical application remained. The finding that the vast majority of the articles were about education and training rather than clinical applications suggests that several issues need be addressed for clinical application of surgical simulation...
June 22, 2017: Neurologia Medico-chirurgica
https://www.readbyqxmd.com/read/28632909/catalyst-controlled-enantioselective-and-diastereodivergent-conjugate-addition-of-aldehydes-to-electron-deficient-olefins
#6
Jennifer Kan, Hiroki Maruyama, Matsujiro Akakura, Taichi Kano, Keiji Maruoka
A chiral amine-catalyzed, enantioselective and diastereodivergent method for aldehyde addition to electron deficient olefins is presented. Hydrogen bonding was used as a control element to achieve unusual anti-selectivity, which was further elucidated through mechanistic and computational studies.
June 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28630262/dipeptidyl-peptidase-4-inhibitor-anagliptin-prevents-intracranial-aneurysm-growth-by-suppressing-macrophage-infiltration-and-activation
#7
Taichi Ikedo, Manabu Minami, Hiroharu Kataoka, Kosuke Hayashi, Manabu Nagata, Risako Fujikawa, Sei Higuchi, Mika Yasui, Tomohiro Aoki, Miyuki Fukuda, Masayuki Yokode, Susumu Miyamoto
BACKGROUND: Chronic inflammation plays a key role in the pathogenesis of intracranial aneurysms (IAs). DPP-4 (dipeptidyl peptidase-4) inhibitors have anti-inflammatory effects, including suppressing macrophage infiltration, in various inflammatory models. We examined whether a DPP-4 inhibitor, anagliptin, could suppress the growth of IAs in a rodent aneurysm model. METHODS AND RESULTS: IAs were surgically induced in 7-week-old male Sprague Dawley rats, followed by oral administration of 300 mg/kg anagliptin...
June 19, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28624505/deficiency-in-ep4-receptor-associated-protein-ameliorates-abnormal-anxiety-like-behavior-and-brain-inflammation-in-a-mouse-model-of-alzheimer-disease
#8
Risako Fujikawa, Sei Higuchi, Masato Nakatsuji, Mika Yasui, Taichi Ikedo, Manabu Nagata, Kosuke Hayashi, Masayuki Yokode, Manabu Minami
Microglia are thought to play key roles in the progression of Alzheimer disease (AD). Overactivated microglia produce proinflammatory cytokines, such as tumor necrosis factor-α, which appear to contribute to disease progression. Previously, we reported that prostaglandin E2 type 4 receptor-associated protein (EPRAP) promotes microglial activation. We crossed human amyloid precursor protein transgenic mice from strain J20(+/-) onto an EPRAP-deficient background to determine the role of EPRAP in AD. Behavioral tests were performed in 5-month-old male J20(+/-)EPRAP(+/+) and J20(+/-)EPRAP(-/-) mice...
June 15, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28620946/incidental-detection-of-asymptomatic-migration-of-hem-o-lok-clip-into-the-bladder-after-laparoscopic-radical-prostatectomy
#9
Junpei Iizuka, Yasunobu Hashimoto, Tsunenori Kondo, Toshio Takagi, Keisuke Hata, Taichi Kanzawa, Hidekazu Tachibana, Kazuhiko Yoshida, Kazunari Tanabe
Hem-o-lok clips have been widely used in laparoscopic or robot-assisted surgery. We report a case of an incidentally discovered Hem-o-lok migration into the bladder after laparoscopic radical prostatectomy. The patient was a 75-year-old man with localized prostate cancer who underwent laparoscopic radical prostatectomy in July 2009. At 3 postoperative years, follow-up ultrasonography revealed a small round mass in the bladder. No lower urinary tract symptoms were reported, and urinalysis results had never indicated hematuria or pyuria...
June 15, 2017: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/28618498/magnetohydrodynamics-of-laser-produced-high-energy-density-plasma-in-a-strong-external-magnetic-field
#10
Kazuki Matsuo, Hideo Nagatomo, Zhe Zhang, Philippe Nicolai, Takayoshi Sano, Shohei Sakata, Sadaoki Kojima, Seung Ho Lee, King Fai Farley Law, Yasunobu Arikawa, Youichi Sakawa, Taichi Morita, Yasuhiro Kuramitsu, Shinsuke Fujioka, Hiroshi Azechi
Recent progress in the generation in the laboratory of a strong (>100-T) magnetic field enables us to investigate experimentally unexplored magnetohydrodynamics phenomena of a high-energy-density plasma, which an external magnetic field of 200-300 T notably affects due to anisotropic thermal conduction, even when the magnetic field pressure is much lower than the plasma pressure. The external magnetic field reduces electron thermal conduction across the external magnetic field lines because the Larmor radius of the thermal electrons in the external magnetic field is much shorter than the mean free path of the thermal electrons...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28597346/the-rate-of-facial-nerve-dysfunction-and-time-to-recovery-after-intraparotid-and-extraparotid-facial-nerve-exposure-and-protection-in-head-and-neck-cutaneous-tumor-surgery
#11
Yasuhiro Nakamura, Yukiko Teramoto, Yuri Asami, Taichi Imamura, Sayuri Sato, Ryota Tanaka, Hiroshi Maruyama, Yoshiyuki Nakamura, Yasuhiro Fujisawa, Manabu Fujimoto, Akifumi Yamamoto
BACKGROUND: Most patients with head and neck skin tumors present with normal facial nerve function. A common treatment strategy for these patients is facial nerve preservation surgery, although the degree to which the nerve is successfully preserved is still unclear. Data on the incidence and recovery of facial nerve dysfunction are woefully lacking in the field of dermato-oncology. METHODS: In 23 patients with normal preoperative facial nerve function, we retrospectively reviewed twenty-six head and neck surgical interventions that included facial nerve exposure and protection, focusing particularly on the differences in outcome between intraparotid and extraparotid exposure of the facial nerve branches...
June 9, 2017: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28592753/recent-surgical-results-for-active-endocarditis-complicated-with-perivalvular-abscess
#12
Daisuke Yoshioka, Koichi Toda, Jun-Ya Yokoyama, Ryohei Matsuura, Shigeru Miyagawa, Yukitoshi Shirakawa, Toshiki Takahashi, Taichi Sakaguchi, Hirotsugu Fukuda, Yoshiki Sawa
BACKGROUND: Surgical treatment for endocarditis patients with a perivalvular abscess is still challenging.Methods and Results:From 2009 to 2016, 470 patients underwent surgery for active endocarditis at 11 hospitals. Of these, 226 patients underwent aortic valve surgery. We compared the clinical results of 162 patients without a perivalvular abscess, 37 patients who required patch reconstruction of the aortic annulus (PR group) and 27 who underwent aortic root replacement (ARR group)...
June 8, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28581717/determination-of-pahs-in-solution-with-a-single-reference-standard-by-a-combination-of-1-h-quantitative-nmr-spectroscopy-and-chromatography
#13
Yuko Kitamaki, Naoki Saito, Taichi Yamazaki, Satoko Otsuka, Satoe Nakamura, Yuzo Nishizaki, Naoki Sugimoto, Masahiko Numata, Toshihide Ihara
We applied a combination of (1)H quantitative NMR spectroscopy ((1)H-qNMR) and chromatography (GC or LC) to establish reliable analytical methods (qNMR/GC and qNMR/LC) for organic compounds. In this method, a reference standard is used as an internal standard for both (1)H-qNMR and chromatography to estimate relative molar sensitivity (RMS) for analytes. The RMSs are calculated from the molar ratios between analytes and the reference standard obtained by (1)H-qNMR; and the response ratio between them obtained by chromatography...
June 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28577229/automated-histological-classification-of-whole-slide-images-of-gastric-biopsy-specimens
#14
Hiroshi Yoshida, Taichi Shimazu, Tomoharu Kiyuna, Atsushi Marugame, Yoshiko Yamashita, Eric Cosatto, Hirokazu Taniguchi, Shigeki Sekine, Atsushi Ochiai
BACKGROUND: Automated image analysis has been developed currently in the field of surgical pathology. The aim of the present study was to evaluate the classification accuracy of the e-Pathologist image analysis software. METHODS: A total of 3062 gastric biopsy specimens were consecutively obtained and stained. The specimen slides were anonymized and digitized. At least two experienced gastrointestinal pathologists evaluated each slide for pathological diagnosis...
June 2, 2017: Gastric Cancer
https://www.readbyqxmd.com/read/28572674/cytokine-profiling-in-the-sub-silicone-oil-fluid-after-vitrectomy-surgeries-for-refractory-retinal-diseases
#15
Hiroki Kaneko, Kei Takayama, Tetsu Asami, Yasuki Ito, Taichi Tsunekawa, Takeshi Iwase, Yasuhito Funahashi, Shinji Ueno, Norie Nonobe, Shunsuke Yasuda, Ayana Suzumura, Hideyuki Shimizu, Reona Kimoto, Shiang-Jyi Hwang, Hiroko Terasaki
Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named the "sub-silicone oil fluid (SOF)", enhances these biological responses. We proposed a safe method for SOF extraction. We also analysed inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular hole-associated retinal detachment (MHRD)...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28566643/prolapse-volume-to-prolapse-height-ratio-for-differentiating-barlow-s-disease-from-fibroelastic-deficiency
#16
Nobuyuki Kagiyama, Misako Toki, Akihiro Hayashida, Minako Ohara, Atsushi Hirohata, Keizo Yamamoto, Toshinori Totsugawa, Taichi Sakaguchi, Kiyoshi Yoshida, Mitsuaki Isobe
BACKGROUND: As mitral valve (MV) repair for Barlow's disease remains surgically challenging, it is important to distinguish Barlow's disease from fibroelastic deficiency (FED) preoperatively. We hypothesized that the prolapse volume to prolapse height ratio (PV-PH ratio) may be useful to differentiate Barlow's disease and FED.Methods and Results:In 76 patients with MV prolapse who underwent presurgical transesophageal echocardiography, the 3D MV morphology was quantified: 19 patients were diagnosed with Barlow's disease and 57 with FED...
May 31, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28559016/critical-roles-of-wnt5a-ror2-signaling-in-aggressiveness-of-tongue-squamous-cell-carcinoma-and-production-of-matrix-metalloproteinase-2-via-%C3%AE-np63%C3%AE-mediated-epithelial-mesenchymal-transition
#17
Taiki Sakamoto, Shintaro Kawano, Ryota Matsubara, Yuichi Goto, Teppei Jinno, Yasuyuki Maruse, Naoki Kaneko, Yuma Hashiguchi, Taichi Hattori, Shoichi Tanaka, Ryoji Kitamura, Tamotsu Kiyoshima, Seiji Nakamura
OBJECTIVES: We previously showed that ΔNp63β, a splicing variant of ΔNp63, mediated EMT and affected cell motility. DNA microarray was thus performed to elucidate the mechanism that ΔNp63β affects cell motility. As the results, Wnt5a was significantly down-regulated by ΔNp63β overexpression in tongue SCC cell line (SQUU-B) with EMT phenotype. MATERIALS AND METHODS: Seven OSCC cell lines were used. Expression of ΔNp63, Wnt5a, its receptor Ror2, and matrix metalloproteinases (MMPs) were analyzed by RT-PCR, real-time PCR, and western blotting, and gelatin zymography...
June 2017: Oral Oncology
https://www.readbyqxmd.com/read/28545300/large-gap-magnetic-topological-heterostructure-formed-by-subsurface-incorporation-of-a-ferromagnetic-layer
#18
Toru Hirahara, Sergey V Eremeev, Tetsuroh Shirasawa, Yuma Okuyama, Takayuki Kubo, Ryosuke Nakanishi, Ryota Akiyama, Akari Takayama, Tetsuya Hajiri, Shin-Ichiro Ideta, Masaharu Matsunami, Kazuki Sumida, Koji Miyamoto, Yasumasa Takagi, Kiyohisa Tanaka, Taichi Okuda, Toshihiko Yokoyama, Shin-Ichi Kimura, Shuji Hasegawa, Evgueni V Chulkov
Inducing magnetism into topological insulators is intriguing for utilizing exotic phenomena such as the quantum anomalous Hall effect (QAHE) for technological applications. While most studies have focused on doping magnetic impurities to open a gap at the surface-state Dirac point, many undesirable effects have been reported to appear in some cases that makes it difficult to determine whether the gap opening is due to the time-reversal symmetry breaking or not. Furthermore, the realization of the QAHE has been limited to low temperatures...
June 14, 2017: Nano Letters
https://www.readbyqxmd.com/read/28533434/lysophosphatidylinositol-induced-activation-of-the-cation-channel-trpv2-triggers-glucagon-like-peptide-1-secretion-in-enteroendocrine-l-cells
#19
Kazuki Harada, Tetsuya Kitaguchi, Taichi Kamiya, Kyaw Htet Aung, Kazuaki Nakamura, Kunihiro Ohta, Takashi Tsuboi
The lysophosphatidylinositol (LPI) has crucial roles in multiple physiological processes, including insulin exocytosis from pancreatic islets. However, the role of LPI in secretion of glucagon like peptide-1 (GLP-1), a hormone that enhances glucose-induced insulin secretion, is unclear. Here, we used the murine enteroendocrine L cell line GLUTag and primary murine small intestinal cells to elucidate the mechanism of LPI-induced GLP-1 secretion. Exogenous LPI addition increased intracellular Ca2+ concentrations ([Ca2+]i) in GLUTag cells and induced GLP-1 secretion from both GLUTag and acutely prepared primary intestinal cells...
May 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28530634/adsorbate-doping-of-mos2-and-wse2-the-influence-of-na-and-co
#20
Takashi Komesu, Duy Le, Iori Tanabe, Eike F Schwier, Yohei Kojima, Mingtian Zheng, Kazuaki Taguchi, Koji Miyamoto, Taichi Okuda, Hideaki Iwasawa, Kenya Shimada, Talat S Rahman, Peter A Dowben
We have investigated the influence of metal adsorbates (sodium and cobalt) on the occupied and unoccupied electronic structure of MoS2(0 0 0 1) and WSe2(0 0 0 1), through a combination of both photoemission and inverse photoemission. The electronic structure is rigidly shifted in both the WSe2 and MoS2 systems, with either Na or Co adsorption, generally as predicted by accompanying density functional theory based calculations. Na adsorption is found to behave as an electron donor (n-type) in MoS2, while Co adsorption acts as an electron acceptor (p-type) in WSe2...
July 19, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
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