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Akira Oike, Koichiro Watanabe, Mi-Sook Min, Koji Tojo, Masahide Kumagai, Yuya Kimoto, Tadashi Yamashiro, Takanori Matsuo, Maho Kodama, Yoriko Nakamura, Masaru Notsu, Takeyoshi Tochimoto, Hiroyuki Fujita, Maki Ota, Etsuro Ito, Shigeki Yasumasu, Masahisa Nakamura
Each vertebrate species, as a general rule, has either the XX/XY or ZZ/ZW chromosomes by which sex is determined. However, the Japanese Rana (R.) rugosa frog is an exception, possessing both sex-determining combinations within one species, varying with region of origin. We collected R. rugosa frogs from 104 sites around Japan and South Korea and determined the nucleotide sequences of the mitochondrial 12S ribosomal RNA gene. Based on the sequences, R. rugosa frogs were divided into four groups from Japan and one from South Korea...
August 2017: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
Hagit Ofir, Hadas Shefler, Rachel Kornhaber, Michelle Cleary, Moti Harats, Josef Haik
The opening of the National Burns Center (NBC) in Sheba Medical Center (SMC) in 2014 was a keystone point in the development of specialized burns trauma services for Israel to ensure burn care and disaster planning at international standards. The NBC is a purpose-built, level 1 burns trauma center that receives patients from Israel, Gaza, West Bank, and abroad. Hosting six intensive care and four step-down burns beds, the center serves as a referral center for patients in other health-care facilities with indication for hospitalization...
January 22, 2018: Nursing & Health Sciences
Abiodun T Seriki, Stella I Smith, Adeyemi I Adeleye, Muinah A Fowora
OBJECTIVE: To determine the occurrence of 16S rRNA mutations associated with low-level tetracycline resistance in H. pylori isolates from adult dyspeptic patients in South-west, Nigeria. METHODS: Antimicrobial susceptibility testing of fifty isolates was determined by E-test for tetracycline. The conserved region (535bp) of the H. pylori tetracycline binding sites of 16S rRNA was amplified by PCR, followed by sequencing and multiple sequence alignment of all the fifty clinical isolates studied...
January 17, 2018: Journal of Global Antimicrobial Resistance
Krishnamurthy Konduru, Amy C Shurtleff, Sina Bavari, Gerardo Kaplan
Ebola virus (EBOV), classified as a category A agent by the CDC and NIH, requires BSL-4 containment and induces high morbidity and mortality in humans. The 2013-2015 epidemic in West Africa underscored the urgent need to develop vaccines and therapeutics to prevent and treat EBOV disease. Neutralization assays are needed to evaluate the efficacy of EBOV vaccines and antibody therapies. Pseudotyped viruses based on nonpathogenic or attenuated vectors reduce the risks involved in the evaluation of neutralizing antibodies against highly pathogenic viruses...
January 16, 2018: Journal of Virological Methods
Dana Atzil-Slonim, Eran Bar-Kalifa, Hadar Fisher, Tuvia Peri, Wolfgang Lutz, Julian Rubel, Eshkol Rafaeli
The present study aimed to (a) explore 2 indices of emotional congruence-temporal similarity and directional discrepancy-between clients' and therapists' ratings of their emotions as they cofluctuate session-by-session; and (b) examine whether client/therapist emotional congruence predicts clients' symptom relief and improved functioning. The sample comprised 109 clients treated by 62 therapists in a university setting. Clients and therapists self-reported their negative (NE) and positive emotions (PE) after each session...
January 2018: Journal of Counseling Psychology
Nathanael West, Suzanne J Snodgrass, Carole James
BACKGROUND: Limited literature exists investigating biomechanical changes during a Functional Capacity Evaluation (FCE). OBJECTIVE: To determine change in joint angle measurement between minimum load to safe maximum load in the bench to shoulder lift of the WorkHab FCE. METHODS: Dartfish ProSuite was used to analyse bench to shoulder lift video from 28 subjects. Measurements of joint angle at lumbar spine, thoracic spine, elbow and shoulder at four points in the ascending and descending lift phases (0/3, 1/3, 2/3, 3/3) in the minimum load and safe maximum lift were collected...
January 18, 2018: Work: a Journal of Prevention, Assessment, and Rehabilitation
Bess L Caswell, Sameera A Talegawkar, Ward Siamusantu, Keith P West, Amanda C Palmer
Inadequate nutrient intakes put children at risk for impaired growth and development. We described diet, usual intakes of energy and macro- and micronutrients and prevalence of nutrient intake adequacies among 4-8-year-old Zambian children. Children not yet in school and living in Mkushi District, Central Province, Zambia were enrolled into an efficacy trial of pro-vitamin A biofortified maize. Children in the non-intervened arm were included in this analysis (n 202). Dietary intake data were collected by tablet-based 24-h recall on a monthly basis over the 6-month trial...
January 2018: British Journal of Nutrition
Yujie Sun, Baoyan Liu, Liyun He, Xiaodong Wu, Jia Liu
Acupuncture is developing rapidly in the world, and more attention is paid on acupuncture in various countries. Because of the cultural differences, there are different views on acupuncture between China and the west, which has brought influence and challenge to the development of acupuncture in the world. Acupuncture-related research is becoming increasingly extensive and complex, but the definition of acupuncture is lack of unified standards. The definition of acupuncture is in urgent need. Based on the analysis of acupuncture definition in the 201 international organizations of 48 countries on five continents and legislation of representative countries, this paper summarized the development status of acupuncture in foreign countries, and put forward that the definition of acupuncture should adopt the model of small connotation and large extension, integrate discipline superiority, expand the scope of acupuncture, and focus on the overall situation...
December 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Xiumei Ren
Acupuncture in Chinese medicine has been a treasure in Chinese traditional medicine for thousands of years. It is opposite to many basic theories in modern medicine in dynasties. Different from the cross compatibility and interactions among medical medicines, acupuncture in Chinese medicine has its own consolidation and inherent philosophical ideas. In view of this, how to discuss the philosophical ideas and its development of acupuncture in Chinese medicine becomes of great importance. It is crucial to clearly answer the three theoretical propositions in the development of acupuncture in Chinese medicine...
December 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Zheng Sun, Jianbin Zhang
The ancient and modern literature relevant with the governor vessel disorders is collected systematically and their connotation is reconstructed. After the collection and analysis on the ancient and modern medical books and literature relevant with the governor vessel disorders, it is discovered that the governor vessel disorders in the ancient time are relevant with the running course of the governor vessel, organs and dysfunction. While the governor vessel disorders in modern time are involved with the spine, nerve, respiration, digestion, urinary reproduction, head, face and five sensory organs, etc...
October 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Kim Bloomfield, Ulrike Grittner, Ludwig Kraus, Daniela Piontek
Aim: A drinking pattern is not only a major drinking variable, but is also one indicator of a country's drinking culture. In the present study, we examine drinking patterns within and across the neighbouring countries of Denmark and Germany. The aim of the research is to determine to what extent drinking patterns differ or are shared at the sub-national level in the two countries. Method: Data came from the German 2012 Epidemiological Survey of Substance Use (n 9084) 18-64 years (response rate 54%), and the Centre for Alcohol and Drug Research's 2011 Danish national survey (n = 5133) 15-79 years (response rate 64%), which was reduced to a common age range, producing a final of 4016...
August 2017: Nordisk Alkohol- & Narkotikatidskrift: NAT
Matthew T Boyd
Three grid-connected monocrystalline silicon arrays on the National Institute of Standards and Technology (NIST) campus in Gaithersburg, MD have been instrumented and monitored for 1 yr, with only minimal gaps in the data sets. These arrays range from 73 kW to 271 kW, and all use the same module, but have different tilts, orientations, and configurations. One array is installed facing east and west over a parking lot, one in an open field, and one on a flat roof. Various measured relationships and calculated standard metrics have been used to compare the relative performance of these arrays in their different configurations...
February 2018: Journal of Solar Energy Engineering
Kuo-Tsang Huang, Shing-Ru Yang, Andreas Matzarakis, Tzu-Ping Lin
The outdoor thermal environment is expected to be deteriorated under climate change. An approach of risk identification including assessment from aspects of thermal stress effect, people's exposure, and local's vulnerability were adopted to study a hot-and-humid traditional rural community located at Tainan, Taiwan. Layers of each aspect were either constructed by in-situ measurements or simulations. To evaluate the future thermal comfort changes by simulations, the prerequisite hourly climate data of three future time slices were produced...
January 17, 2018: Science of the Total Environment
Yu-Cheng Chen, Chun-Kuei Yao, Tsuyoshi Honjo, Tzu-Ping Lin
The effects of urban heat island (UHI) have recently become a crucial issue. This study utilized a high-density street-level air temperature observation network (HiSAN) to understand the UHI characteristics in Tainan City. A total of 100 measurement points were established throughout the city. The average distance between two neighboring measuring points was 1.9 km in rural areas and 0.8 km in metropolitan areas. The UHI caused a temperature differences of at least 3 °C in each month over the study period, and the UHI's centric point moved from west to east during the day and from east to west at night, mainly because of the physical effects of the different urban environment including location and the impermeable surface area (ISA), total floor area, and sky view factor in urban areas...
January 17, 2018: Science of the Total Environment
Ryan West, Thomas Cunningham, Lewis E Pennicott, Srinivasa P S Rao, Simon E Ward
New tools are required to ensure the adequate control of the neglected tropical disease human African trypanosomia-sis. Annual reports of infection have recently fallen to fewer than 5000 cases per year; however, current therapies are hard to administer and have safety concerns, hence are far from ideal. Trypanosome alternative oxidase is an exciting target for controlling the infection; it is unique to the parasite and inhibition of this enzyme with the natural product ascofuranone has shown to clear in vivo infections...
January 21, 2018: ACS Infectious Diseases
Mahsa Mousavi-Derazmahalleh, Philipp E Bayer, Bruno Nevado, Bhavna Hurgobin, Dmitry Filatov, Andrzej Kilian, Lars G Kamphuis, Karam B Singh, Jens D Berger, James K Hane, David Edwards, William Erskine, Matthew N Nelson
This first pan-Mediterranean analysis of genetic diversity in wild narrow-leafed lupin revealed strong East-West genetic differentiation of populations, an historic eastward migration, and signatures of genetic adaptation to climatic variables. Most grain crops suffer from a narrow genetic base, which limits their potential for adapting to new challenges such as increased stresses associated with climate change. Plant breeders are returning to the wild ancestors of crops and their close relatives to broaden the genetic base of their crops...
January 20, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
For Yue Tso, Andrew V Kossenkov, Salum J Lidenge, Owen Ngalamika, John R Ngowi, Julius Mwaiselage, Jayamanna Wickramasinghe, Eun Hee Kwon, John T West, Paul M Lieberman, Charles Wood
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma (KS). It is endemic in a number of sub-Saharan African countries with infection rate of >50%. The high prevalence of HIV-1 coupled with late presentation of advanced cancer staging make KS the leading cancer in the region with poor prognosis and high mortality. Disease markers and cellular functions associated with KS tumorigenesis remain ill-defined. Several studies have attempted to investigate changes of the gene profile with in vitro infection of monoculture models, which are not likely to reflect the cellular complexity of the in vivo lesion environment...
January 19, 2018: PLoS Pathogens
Xianli Che, Min Zhang, Yanyan Zhao, Qiang Zhang, Qing Quan, Anders Møller, Fasheng Zou
Ecological differences may be related to community component divisions between Oriental (west) and Sino-Japanese (east) realms, and such differences may result in weak geographical breaks in migratory species that are highly mobile. Here, we conducted comparative phylogenetic and functional structure analyses of wintering waterbird communities in southern China across two realms and subsequently examined possible climate drivers of the observed patterns. An analysis based on such highly migratory species is particularly telling because migration is bound to reduce or completely eliminate any divergence between communities...
January 19, 2018: Scientific Reports
Tú Nguyen-Dumont, Aleksander Myszka, Pawel Karpinski, Maria M Sasiadek, Hayane Akopyan, Fleur Hammet, Helen Tsimiklis, Daniel J Park, Bernard J Pope, Ryszard Slezak, Nataliya Kitsera, Aleksandra Siekierzynska, Melissa C Southey
BACKGROUND: FANCM and RECQL have recently been reported as breast cancer susceptibility genes and it has been suggested that they should be included on gene panel tests for breast cancer predisposition. However, the clinical value of testing for mutations in RECQL and FANCM remains to be determined. In this study, we have characterised the spectrum of FANCM and RECQL mutations in women affected with breast or ovarian cancer from South-West Poland and West Ukraine. METHODS: We applied Hi-Plex, an amplicon-based enrichment method for targeted massively parallel sequencing, to screen the coding exons and proximal intron-exon junctions of FANCM and RECQL in germline DNA from unrelated women affected with breast cancer (n = 338) and ovarian cancer (n = 89) from Poland (n = 304) and Ukraine (n = 123)...
January 19, 2018: BMC Medical Genetics
John B West, Daniel L Wang, Gordon Kim Prisk
We are developing a novel, noninvasive method for measuring the efficiency of pulmonary gas exchange in patients with lung disease. The patient wears an oximeter, and we measure the partial pressures of oxygen and carbon dioxide in inspired and expired gas using miniature analyzers. The arterial PO2 is then calculated from the oximeter reading and the oxygen dissociation curve, using the end-tidal PCO2 to allow for the Bohr effect. This calculation is only accurate when the oxygen saturation is below 94% and therefore these normal subjects breathed 12...
December 20, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
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