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https://www.readbyqxmd.com/read/28640922/changes-in-hemp-secondary-fiber-production-related-to-technical-fiber-variability-revealed-by-light-microscopy-and-attenuated-total-reflectance-fourier-transform-infrared-spectroscopy
#1
Eva Fernandez-Tendero, Arnaud Day, Sandrine Legros, Anouck Habrant, Simon Hawkins, Brigitte Chabbert
Interest in hemp (Cannabis sativa L.) is increasing due to the development of a new range of industrial applications based on bast fibers. However the variability of bast fiber yield and quality represents an important barrier to further exploitation. Primary and secondary fiber content was examined in two commercial hemp varieties (Fedora 17, Santhica 27) grown under contrasted sowing density and irrigation conditions. Both growing conditions and hemp varieties impact stem tissue architecture with a large effect on the proportion of secondary fibers but not primary fibers...
2017: PloS One
https://www.readbyqxmd.com/read/28636437/tobacco-rattle-virus-trv-mediated-silencing-of-nicotiana-benthamiana-argonautes-nbagos-reveals-new-antiviral-candidates-and-dominant-effects-of-trv-nbago1
#2
Denis Odokonyero, Maria R Mendoza, Peter Moffett, Herman B Scholthof
The objective of this study was to determine the contribution of different ARGONAUTE proteins in Nicotiana benthamiana (NbAGOs) to the defense against silencing sensitive GFP-expressing viral constructs based on Tomato bushy stunt virus (TBSV) (Tombusvirus), Sunn-hemp mosaic virus (Tobamovirus), and Foxtail mosaic virus (Potexvirus). Upon Tobacco rattle virus (TRV)-mediated down-regulation of NbAGO1, 4, 5, or 6, no effects were noted on susceptibility to any virus construct, whereas knockdown of NbAGO2 specifically prevented silencing of P19-defective TBSV (TGdP19)...
June 21, 2017: Phytopathology
https://www.readbyqxmd.com/read/28625994/a-small-protein-hemp-is-a-transcriptional-activator-for-hemin-uptake-operon-in-burkholderia-multivorans-atcc-17616
#3
Takuya Sato, Shouta Nonoyama, Akane Kimura, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda
Iron and heme play very important roles in various metabolic functions in bacteria, and their intracellular homeostasis is maintained because high concentrations of free forms of these molecules greatly facilitate the Fenton reaction-mediated production of large amounts of reactive oxygen species that severely damage various biomolecules. Ferric uptake regulator (Fur) from Burkholderiamultivorans ATCC 17616 is an iron-responsive global transcriptional regulator, and its fur deletant exhibits pleiotropic phenotypes...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28610096/the-genus-i-phlesirtes-i-bolivar-1922-orthoptera-tettigoniidae-conocephalinae-conocephalini-karniellina-a-review-of-the-genus-with-data-on-its-bioacoustics-and-the-description-of-new-species
#4
Claudia Hemp, Klaus-Gerhard Heller
Species of the genus Phlesirtes are reviewed in this paper and 16 new species described. A key is provided to the species of Phlesirtes. Among the subtribe Karniellina of Conocephalini members of the genus Phlesirtes occupy habitats of montane to afroalpine grasslands in East Africa. All recorded species produced long-lasting, trill-like calling songs, consisting of sequences of continuously repeated syllables or syllable pairs with the peak of the carrier frequency in the low ultrasound.
March 22, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609870/revision-of-the-genus-amytta-orthoptera-tettigoniidae-meconematinae-and-new-species-from-east-africa
#5
Claudia Hemp, Klaus-Gerhard Heller
New species of Amytta are described from East Africa. Beside tegminal length, they differ mainly in the structure of the complicated male genitalic organs, notably the last abdominal tergite and cerci. Data on habitat and the acoustics of some species and a key to the species are provided. The songs of all seven Amytta species studied are very similar, consisting of long trills with a syllable repetition rate of about 60 Hz (at 20ºC) and a broad frequency peak in the ultrasound at around 40 kHz. The biogeographic pattern and phylogenetic relationships are discussed for the group...
May 9, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609672/development-of-a-new-extraction-technique-and-hplc-method-for-the-analysis-of-non-psychoactive-cannabinoids-in-fibre-type-cannabis-sativa-l-hemp
#6
Virginia Brighenti, Federica Pellati, Marleen Steinbach, Davide Maran, Stefania Benvenuti
The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed to identify samples with a high content of bioactive compounds, with a view to underscoring the importance of quality control in derived products as well. Different extraction methods, including dynamic maceration (DM), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical-fluid extraction (SFE) were applied and compared in order to obtain a high yield of the target analytes from hemp...
June 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28549545/developmental-and-internal-validation-of-a-novel-13-loci-str-multiplex-method-for-cannabis-sativa-dna-profiling
#7
Rachel Houston, Matthew Birck, Sheree Hughes-Stamm, David Gangitano
Marijuana (Cannabis sativa L.) is a plant cultivated and trafficked worldwide as a source of fiber (hemp), medicine, and intoxicant. The development of a validated method using molecular techniques such as short tandem repeats (STRs) could serve as an intelligence tool to link multiple cases by means of genetic individualization or association of cannabis samples. For this purpose, a 13 loci STR multiplex method was developed, optimized, and validated according to relevant ISFG and SWGDAM guidelines. The STR multiplex consists of 13 previously described C...
May 2017: Legal Medicine
https://www.readbyqxmd.com/read/28482156/mno2-nanowire-biomass-derived-carbon-from-hemp-stem-for-high-performance-supercapacitors
#8
MinHo Yang, Dong Seok Kim, Seok Bok Hong, Jae-Wook Sim, Jinsoo Kim, Seung-Soo Kim, Bong Gill Choi
Hierarchical 3D nanostructures based on waste biomass are being offered as promising materials for energy storage due to their processabilities, multifunctionalities, environmental benignities, and low cost. Here we report a facile, inexpensive, and scalable strategy for the fabrication of hierarchical porous 3D structure as electrode materials for supercapacitors based on MnO2 nanowires and hemp-derived activated carbon (HC). Vertical MnO2 wires are uniformly deposited onto the surface of HC using a one-step hydrothermal method to produce hierarchical porous structures with conductive interconnected 3D networks...
May 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28457055/short-term-efficacy-of-cbd-enriched-hemp-oil-in-girls-with-dysautonomic-syndrome-after-human-papillomavirus-vaccination
#9
Beniamino Palmieri, Carmen Laurino, Maria Vadalà
BACKGROUND: Cannabidiol (CBD)-based treatments for several diseases, including Tourette's syndrome, multiple sclerosis, epilepsy, movement disorders and glaucoma, are proving to be beneficial and the scientific clinical background of the drug is continuously evolving. OBJECTIVES: To investigate the short-term effect of CBD-enriched hemp oil for relieving symptoms and improving the life quality (QOL) in young girls with adverse drug effects (ADRs) following human papillomavirus (HPV) vaccine...
February 2017: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/28448475/formulation-characterization-and-properties-of-hemp-seed-oil-and-its-emulsions
#10
Veronika Mikulcová, Věra Kašpárková, Petr Humpolíček, Leona Buňková
The formulation, characterization, and anticipated antibacterial properties of hemp seed oil and its emulsions were investigated. The oil obtained from the seeds of Cannabis sativa L. in refined and unrefined form was characterized using iodine, saponification, acid values, and gas chromatography, and was employed for the preparation of stable oil-in-water emulsions. The emulsions were prepared using pairs of non-ionic surfactants (Tween, Span). The effects of the emulsification method (spontaneous emulsification vs...
April 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444789/effect-of-dietary-supplementation-of-hemp-seed-cannabis-sativa-l-on-meat-quality-and-egg-fatty-acid-composition-of-japanese-quail-coturnix-coturnix-japonica
#11
H Yalcin, Y Konca, F Durmuscelebi
This study was conducted in two separate experiments. Experiment I was conducted to investigate the effects of hemp seed (HS) on meat quality traits, and experiment II was designed to determine egg fatty acid (FA) composition in Japanese quail (Coturnix coturnix japonica). In experiment I and experiment II treatments, groups were as follows: (i) control (not included HS0), (ii) 5% HS inclusion to diets, (iii) 10% HS inclusion to diets and (iv) 20% HS inclusion to diets. In experiment I, a total of 192 7-day-old-quail were fed with HS diets for 5 weeks, and end of the experiment I, a total of 64 quail (16 each) slaughtered and meat samples were analysed for meat colour, pH, cooking and thawing loss and FA composition...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28435268/biocompatible-nanoemulsions-based-on-hemp-oil-and-less-surfactants-for-oral-delivery-of-baicalein-with-enhanced-bioavailability
#12
Juntao Yin, Cuiyu Xiang, Peiqing Wang, Yuyun Yin, Yantao Hou
Baicalein (BCL) possesses high pharmacological activities but low solubility and stability in the intestinal tract. This study aimed to probe the potential of nanoemulsions (NEs) consisting of hemp oil and less surfactants in ameliorating the oral bioavailability of BCL. BCL-loaded NEs (BCL-NEs) were prepared by high-pressure homogenization technique to reduce the amount of surfactants. BCL-NEs were characterized by particle size, entrapment efficiency (EE), in vitro drug release, and morphology. Bioavailability was studied in Sprague-Dawley rats following oral administration of BCL suspensions, BCL conventional emulsions, and BCL-NEs...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28431313/raman-spectroscopy-for-the-characterization-of-different-fractions-of-hemp-essential-oil-extracted-at-130%C3%A2-c-using-steam-distillation-method
#13
Muhammad Asif Hanif, Haq Nawaz, Saima Naz, Rubina Mukhtar, Nosheen Rashid, Ijaz Ahmad Bhatti, Muhammad Saleem
In this study, Raman spectroscopy along with Principal Component Analysis (PCA) is used for the characterization of pure essential oil (pure EO) isolated from the leaves of the Hemp (Cannabis sativa L.,) as well as its different fractions obtained by fractional distillation process. Raman spectra of pure Hemp essential oil and its different fractions show characteristic key bands of main volatile terpenes and terpenoids, which significantly differentiate them from each other. These bands provide information about the chemical composition of sample under investigation and hence can be used as Raman spectral markers for the qualitative monitoring of the pure EO and different fractions containing different active compounds...
July 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28360330/on-the-origins-of-oxygenic-photosynthesis-and-aerobic-respiration-in-cyanobacteria
#14
Rochelle M Soo, James Hemp, Donovan H Parks, Woodward W Fischer, Philip Hugenholtz
The origin of oxygenic photosynthesis in Cyanobacteria led to the rise of oxygen on Earth ~2.3 billion years ago, profoundly altering the course of evolution by facilitating the development of aerobic respiration and complex multicellular life. Here we report the genomes of 41 uncultured organisms related to the photosynthetic Cyanobacteria (class Oxyphotobacteria), including members of the class Melainabacteria and a new class of Cyanobacteria (class Sericytochromatia) that is basal to the Melainabacteria and Oxyphotobacteria All members of the Melainabacteria and Sericytochromatia lack photosynthetic machinery, indicating that phototrophy was not an ancestral feature of the Cyanobacteria and that Oxyphotobacteria acquired the genes for photosynthesis relatively late in cyanobacterial evolution...
March 31, 2017: Science
https://www.readbyqxmd.com/read/28355238/terpene-synthases-from-cannabis-sativa
#15
Judith K Booth, Jonathan E Page, Jörg Bohlmann
Cannabis (Cannabis sativa) plants produce and accumulate a terpene-rich resin in glandular trichomes, which are abundant on the surface of the female inflorescence. Bouquets of different monoterpenes and sesquiterpenes are important components of cannabis resin as they define some of the unique organoleptic properties and may also influence medicinal qualities of different cannabis strains and varieties. Transcriptome analysis of trichomes of the cannabis hemp variety 'Finola' revealed sequences of all stages of terpene biosynthesis...
2017: PloS One
https://www.readbyqxmd.com/read/28317711/analysis-of-cannabinoids-by-liquid-chromatography-mass-spectrometry-in-milk-liver-and-hemp-seed-to-ensure-food-safety
#16
Úrsula Escrivá, María Jesús Andrés-Costa, Vicente Andreu, Yolanda Picó
A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ(9)-THC (THC-COOH) and 11-hidroxy-Δ(9)-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28275995/comparison-of-traditional-field-retting-and-phlebia-radiata-cel-26-retting-of-hemp-fibres-for-fibre-reinforced-composites
#17
Ming Liu, Marcel T Ale, Bartłomiej Kołaczkowski, Dinesh Fernando, Geoffrey Daniel, Anne S Meyer, Anders Thygesen
Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical properties of the produced fibre/epoxy composites. For field retting a classification of the microbial evolution (by gene sequencing) and enzyme profiles were conducted. By phylogenetic frequency mapping, different types of fungi, many belonging to the Ascomycota phylum were found on the fibres during the first 2 weeks of field retting, and thereafter, different types of bacteria, notably Proteobacteria, also proliferated on the field retted fibres...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28269067/on-the-pleasantness-of-a-haptic-stimulation-how-different-textures-can-be-recognized-through-heart-rate-variability-nonlinear-analysis
#18
Mimma Nardelli, Alberto Greco, Matteo Bianchi, Enzo Pasquale Scilingo, Gaetano Valenza
The hedonic attributes of cutaneous elicitation play a crucial role in everyday life, influencing our behavior and psychophysical state. However, the correlation between such a hedonic aspect of touch and the Autonomic Nervous System (ANS)-related physiological response, which is intimately connected to emotions, still needs to be deeply investigated. This study reports on caress-like stimuli conveyed to the forearm of 32 healthy subjects through different fabrics actuated by a haptic device, which can control both the strength (i...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28268359/investigating-mechanical-properties-of-a-fabric-based-affective-haptic-display-through-electrodermal-activity-analysis
#19
Alberto Greco, Gaetano Valenza, Enzo Pasquale Scilingo
The aim of this study is the development of a classification system able to discern between two levels of velocity of a caress-like haptic stimulus, through information gathered from the analysis of the Electrodermal Activity (EDA) dynamics. We designed and performed an experiment where EDA signals were acquired during caress-like stimuli conveyed to 32 healthy volunteers (16 females) by means of four different fabrics (hemp, burlap, velvet and silk) and at two velocity levels (9.4 mm/s and 65 mm/s). CvxEDA model was used to process the EDA signal and extract features from both tonic and phasic components...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28240903/flexible-bionanocomposites-from-epoxidized-hemp-seed-oil-thermosetting-resin-reinforced-with-halloysite-nanotubes
#20
Peter S Shuttleworth, Ana M Díez-Pascual, Carlos Marco, Gary Ellis
Hemp seed (Cannabis sativa L.) oil comprises a variety of beneficial unsaturated triglycerides with well-documented nutritional and health benefits. However, it can become rancid over a relatively short time period, leading to increased industrial costs and waste of a valuable product. The development of sustainable polymers is presented as a strategy, where both the presence of unsaturation and peroxide content could be effectively used to alleviate both the waste and financial burden. After the reaction with peroxyacetic acid, the incorporation of halloysite nanotubes (HNTs), and the subsequent thermal curing, without the need for organic solvents or interfacial modifiers, flexible transparent materials with a low glass-transition temperature were developed...
March 8, 2017: Journal of Physical Chemistry. B
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