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Phospholipase c

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https://www.readbyqxmd.com/read/28927001/a-review-and-database-of-snake-venom-proteomes
#1
REVIEW
Theo Tasoulis, Geoffrey K Isbister
Advances in the last decade combining transcriptomics with established proteomics methods have made possible rapid identification and quantification of protein families in snake venoms. Although over 100 studies have been published, the value of this information is increased when it is collated, allowing rapid assimilation and evaluation of evolutionary trends, geographical variation, and possible medical implications. This review brings together all compositional studies of snake venom proteomes published in the last decade...
September 18, 2017: Toxins
https://www.readbyqxmd.com/read/28926992/the-native-fruit-geoffroea-decorticans-from-arid-northern-chile-phenolic-composition-antioxidant-activities-and-in-vitro-inhibition-of-pro-inflammatory-and-metabolic-syndrome-associated-enzymes
#2
Felipe Jiménez-Aspee, Cristina Theoduloz, Maria Del Pilar C Soriano, Maider Ugalde-Arbizu, Maria Rosa Alberto, Iris Catiana Zampini, Maria Inés Isla, Mario J Simirigiotis, Guillermo Schmeda-Hirschmann
The native tree Geoffroea decorticans (chañar) grows in the arid lands of northern Chile. It has been used as a food plant since prehistoric times. Phenolic-enriched extracts (PEEs) of Chilean chañar fruits were assessed for their chemical composition, antioxidant properties and inhibition of pro-inflammatory and metabolic syndrome-associated enzymes. Phenolic profiles were determined by HPLC-DAD-MS/MS. The PEEs of G. decorticans showed a strong effect towards the enzymes COX-1/COX-2, with inhibition percentages ranging from inactive to 92...
September 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28926770/1-25-oh-2d3-induces-regulatory-t-cell-differentiation-by-influencing-the-vdr-plc-%C3%AE-1-tgf-%C3%AE-1-pathway
#3
Qiang Zhou, Shengying Qin, Jinyan Zhang, Lin Zhon, Zhihai Pen, Tonghai Xing
Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared to those with vitamin D deficiency. Thus, a high percentage of regulatory T cells might play a key role in preventing acute cellular rejection (ACR). In this report, we studied the specific effects of 1,25(OH)2D3 on human T cell diff ;erentiation, and determined the potential molecule mechanism behind...
September 16, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28926616/co-ordinated-activation-of-classical-and-novel-pkc-isoforms-is-required-for-pma-induced-mtorc1-activation
#4
Mengling Liu, Christopher J Clarke, Mohamed F Salama, Yeon Ja Choi, Lina M Obeid, Yusuf A Hannun
Protein kinase C (PKC) has been shown to activate the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, a central hub in the regulation of cell metabolism, growth and proliferation. However, the mechanisms by which PKCs activate mTORC1 are still ambiguous. Our previous study revealed that activation of classical PKCs (cPKC) results in the perinuclear accumulation of cPKC and phospholipase D2 (PLD2) in recycling endosomes in a PLD2-dependent manner. Here, we report that mTORC1 activation by phorbol 12,13-myristate acetate (PMA) requires both classic, cPKC, and novel PKC (nPKC) isoforms, specifically PKCη, acting through distinct pathways...
2017: PloS One
https://www.readbyqxmd.com/read/28922966/plasma-lipids-metabolism-in-mild-cognitive-impairment-and-alzheimer-s-disease
#5
Alana C Costa, Helena P G Joaquim, Orestes Forlenza, Leda L Talib, Wagner F Gattaz
OBJECTIVES: Expression of phospholipids and related molecules could provide panels of multiple biomarkers searching for the signature of Alzheimer's disease (AD). The aim of the present study was to quantify ten phospholipids and simultaneously determine phospholipase A2 (PLA2) activity in blood of mild cognitive impairment (MCI) and AD patients. METHODS: Thirty-four AD, 20 MCI and 25 controls were enrolled. The phospholipids where analysed using the AbsoluteIDQ(®) p180 Kit...
September 19, 2017: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/28918129/where-do-substrates-of-diacylglycerol-kinases-come-from-diacylglycerol-kinases-utilize-diacylglycerol-species-supplied-from-phosphatidylinositol-turnover-independent-pathways
#6
REVIEW
Fumio Sakane, Satoru Mizuno, Daisuke Takahashi, Hiromichi Sakai
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Mammalian DGK comprises ten isozymes (α-κ) and regulates a wide variety of physiological and pathological events, such as cancer, type II diabetes, neuronal disorders and immune responses. DG and PA consist of various molecular species that have different acyl chains at the sn-1 and sn-2 positions, and consequently, mammalian cells contain at least 50 structurally distinct DG/PA species. Because DGK is one of the components of phosphatidylinositol (PI) turnover, the generally accepted dogma is that all DGK isozymes utilize 18:0/20:4-DG derived from PI turnover...
September 9, 2017: Advances in Biological Regulation
https://www.readbyqxmd.com/read/28917588/association-between-fsp-cvhi-inflammatory-cytokines-and-the-incidence-of-primary-stroke
#7
Xiao-Yun Huang, Wen-Jin Fu, Zhi-Chang Chen, Zhi-Zhong Mei, Ying-Li Yu, Hao-Wei Fang, Yi-Hong Huang, Han Lin, Jian-Jun Chen, Ming-Xia Wang, Shao-Bing Guan, Wan-Xian Lu, Xiao-Ling Chen
This case-control study was designed to establish a new risk-prediction model for primary stroke using Framingham stroke profile (FSP), cerebral vascular hemodynamic indexes (CVHI) and plasma inflammatory cytokines including hs-CRP, IL-6, TNF-α and Lp-PLA2. A total of 101 primary stroke patients admitted to Dongguan Houjie Hospital between August 2014 and June 2015 were assigned into the case group, and 156 age- and gender-matched healthy subjects from the Houjie Community were allocated into the control group...
September 13, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28915869/frequency-and-genotypes-of-chlamydia-trachomatis-in-patients-attending-the-obstetrics-and-gynecology-clinics-in-jalisco-mexico-and-correlation-with-sociodemographic-behavioral-and-biological-factors
#8
Néstor Casillas-Vega, Rayo Morfín-Otero, Santos García, Jorge Llaca-Díaz, Eduardo Rodríguez-Noriega, Adrián Camacho-Ortiz, Ma de la Merced Ayala-Castellanos, Héctor J Maldonado-Garza, Jesús Ancer-Rodríguez, Guadalupe Gallegos-Ávila, Alberto Niderhauser-García, Elvira Garza-González
BACKGROUND: Chlamydia trachomatis is the causative agent of the most common bacterial sexually transmitted infection worldwide. The aim of this study was to investigate the frequency and genotypes of C. trachomatis in patients attending an obstetrics and gynecology clinic in Jalisco, Mexico and correlates them with sociodemographic, behavioral, and biological factors. METHODS: C. trachomatis detection was performed in endocervical samples from 662 patients by direct fluorescence assay (DFA) and two PCR assays that amplified the phospholipase D endonuclease superfamily protein (PLDESP) and OmpA genes...
September 15, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/28914240/comparison-of-candida-species-isolated-from-children-with-and-without-early-childhood-caries-a-descriptive-cross-sectional-study
#9
M S Beena, Faizal C Peedikayil, M B GufranAfmed, T P Chandru, K Soni, N Dhanesh
BACKGROUND: Early childhood caries (ECC) is characterized by the presence of one or more decayed, missing (due to caries), or filled teeth surfaces in any primary tooth, in a child below 6 years of age. Although ECC is primarily associated with high levels of maternal Streptococcus mutans, there has been an increased interest in finding the relationship between oral fungal flora and dental caries. OBJECTIVE: The aim of the study is to identify and characterize the Candida species and to compare the candidal isolates in children with ECC and without ECC...
October 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28911184/subcellular-localization-and-function-study-of-a-secreted-phospholipase-c-from-nocardia-seriolae
#10
Liqun Xia, Haiying Liang, Liang Xu, Jianlin Chen, Michaël Bekaert, Honglian Zhang, Yishan Lu
Fish nocardiosis is a chronic systemic granulomatous disease, and Nocardia seriolae is the main pathogen that causes it. The pathogenesis and virulence factors of N. seriolae are not fully understood. A phospholipase C (PLC), which is likely to be a secreted protein targeting host cell mitochondria, was found by a bioinformatics analysis of the whole genome sequence of N. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC from N. seriolae (NsPLC), in this study gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28906474/non-clinical-studies-for-evaluation-of-8-c-rhamnosyl-apigenin-purified-from-peperomia-obtusifolia-against-acute-edema
#11
Cinthia I Tamayose, Paulete Romoff, Daniela O Toyama, Henrique H Gaeta, Caroline R C Costa, Mariana N Belchor, Bruna D Ortolan, Leosvaldo S M Velozo, Maria A C Kaplan, Marcelo J P Ferreira, Marcos H Toyama
Compound 8-C-rhamnosyl apigenin (8CR) induced a moderate reduction in the enzymatic activity of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus and cytosolic phospholipase A2 (cPLA2), but the compound also significantly inhibited the enzymatic activity of the enzyme cyclooxygenase. In vitro assays showed that the compound induced a slight change in the secondary structure of sPLA2 from Crotalus durissus terrificus snake venom. In vivo assays were divided into two steps. In the first step, the 8CR compound was administered by intraperitoneal injections 30 min prior to administration of sPLA2...
September 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28905664/assessment-of-the-neutralizing-potency-of-antisera-raised-against-native-and-%C3%AE-irradiated-naja-nigricollis-black-necked-spitting-cobra-venom-in-rabbits-concerning-its-cardiotoxic-effect
#12
F Abdou, E E Denshary, E Shaaban, M Mohamed
The present study was designed to prepare a specific safe antiserum for Naja nigricollis using γ-irradiated (1.5KGy and3KGy) venoms. Rabbits were used for active immunization using irradiated venoms (1.5 and 3 kGy) as a toxoid, mice were used for determination of LD50 post immunization and the rats were used for neutralization of the cardiotoxic effect of venom. Results of the immunodiffusion test indicated that the sera of rabbits raised against non-irradiated, 1.5- and 3-kGy γ-irradiated venom, had the same results of precipitin bands...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28903399/phosphatidylcholine-specific-phospholipase-c-inhibition-reduces-her2-overexpression-cell-proliferation-and-in-vivo-tumor-growth-in-a-highly-tumorigenic-ovarian-cancer-model
#13
Luisa Paris, Franca Podo, Francesca Spadaro, Laura Abalsamo, Maria Elena Pisanu, Alessandro Ricci, Serena Cecchetti, Luisa Altabella, Maria Buoncervello, Ludmila Lozneanu, Marina Bagnoli, Carlo Ramoni, Silvana Canevari, Delia Mezzanzanica, Egidio Iorio, Rossella Canese
Antagonizing the oncogenic effects of human epidermal growth factor receptor 2 (HER2) with current anti-HER2 agents has not yet yielded major progress in the treatment of advanced HER2-positive epithelial ovarian cancer (EOC). Using preclinical models to explore alternative molecular mechanisms affecting HER2 overexpression and oncogenicity may lead to new strategies for EOC patient treatment. We previously reported that phosphatidylcholine-specific phospholipase C (PC-PLC) exerts a pivotal role in regulating HER2 overexpression in breast cancer cells...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28902403/s-nitrosylation-denitrosylation-as-a-regulatory-mechanism-of-salt-stress-sensing-in-sunflower-seedlings
#14
Prachi Jain, Christine von Toerne, Christian Lindermayr, Satish C Bhatla
Nitric oxide (NO) and various reactive nitrogen species (RNS) produced in cells in normal growth conditions and their enhanced production under stress conditions, are responsible for a variety of biochemical aberrations. Present findings demonstrate that sunflower seedling roots exhibit high sensitivity to salt stress in terms of nitrite accumulation. A significant reduction in S-nitrosoglutathione reductase (GSNOR) activity is evident in response to salt stress. Restoration of GSNOR activity with dithioerythritol (DTT) shows that the enzyme is reversibly inhibited under conditions of 120 mM NaCl...
September 13, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28900933/neurological-and-motor-disorders-trpc-in-the-skeletal-muscle
#15
Sophie Saüc, Maud Frieden
Transient receptor potential canonical (TRPC) channels belong to the large family of TRPs that are mostly nonselective cation channels with a great variety of gating mechanisms. TRPC are composed of seven members that can all be activated downstream of agonist-induced phospholipase C stimulation, but some members are also stretch-activated and/or are part of the store-operated Ca(2+) entry (SOCE) pathway. Skeletal muscles generate contraction via an explosive increase of cytosolic Ca(2+) concentration resulting almost exclusively from sarcoplasmic reticulum Ca(2+) channel opening...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900918/new-aspects-of-the-contribution-of-er-to-soce-regulation-trpc-proteins-as-a-link-between-plasma-membrane-ion-transport-and-intracellular-ca-2-stores
#16
Alexis Bavencoffe, Michael Xi Zhu, Jin-Bin Tian
Transient receptor potential canonical (TRPC) proteins were identified as molecular candidates of receptor- and/or store-operated channels because of their close homology to the Drosophila TRP and TRPL. Functional studies have revealed that TRPC channels play an integrated part of phospholipase C-transduced cell signaling, mediating the influx of both Ca(2+) and Na(+) into cells. As a consequence, the TRPC channels have diverse functional roles in different cell types, including metabotropic receptor-evoked membrane depolarization and intracellular Ca(2+) concentration elevation...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28894144/molecular-details-of-secretory-phospholipase-a2-from-flax-linum-usitatissimum-l-provide-insight-into-its-structure-and-function
#17
Payal Gupta, Prasanta K Dash
Secretory phospholipase A2 (sPLA2) are low molecular weight proteins (12-18 kDa) involved in a suite of plant cellular processes imparting growth and development. With myriad roles in physiological and biochemical processes in plants, detailed analysis of sPLA2 in flax/linseed is meagre. The present work, first in flax, embodies cloning, expression, purification and molecular characterisation of two distinct sPLA2s (I and II) from flax. PLA2 activity of the cloned sPLA2s were biochemically assayed authenticating them as bona fide phospholipase A2...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28892824/resolvin-d1-increases-mucin-secretion-in-cultured-rat-conjunctival-goblet-cells-via-multiple-signaling-pathways
#18
Marit Lippestad, Robin R Hodges, Tor P Utheim, Charles N Serhan, Darlene A Dartt
Purpose: Goblet cells in the conjunctiva secrete mucin into the tear film protecting the ocular surface. The proresolution mediator resolvin D1 (RvD1) regulates mucin secretion to maintain homeostasis during physiological conditions and in addition, actively terminates inflammation. We determined the signaling mechanisms used by RvD1 in cultured rat conjunctival goblet cells to increase intracellular [Ca2+] ([Ca2+]i) and induce glycoconjugate secretion. Methods: Increase in [Ca2+]i were measured using fura 2/AM and glycoconjugate secretion determined using an enzyme-linked lectin assay with the lectin Ulex Europaeus Agglutinin 1...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28890720/porcine-b-cell-subset-responses-to-toll-like-receptor-ligands
#19
Roman Othmar Braun, Sylvie Python, Artur Summerfield
Toll-like receptors (TLR) triggering of B cells are known to promote B cell expansion, differentiation of B cells into antibody-producing and memory cells, but the TLR responses of porcine B cells is poorly characterized. Therefore, this study investigated the response pattern of porcine B cell subsets to a large collection of TLR ligands and demonstrates that the TLR2 ligand Pam3Cys-SK4 and the TLR7/8 ligands gardiquimod and resiquimod are particularly efficient at inducing proliferation, CD25 and CCR7. This activation was also determined in B-cell subpopulations including a CD21(+)IgM(+) subset, an IgG(+) subset and two putative B1-like subsets, defined as CD21(-)IgM(high)CD11R1(+)CD11c(+)CD14(+) and CD21(-)IgM(high) CD11R1(-)CD11c(+)CD14(-) B cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28887301/the-phospholipase-pnpla7-functions-as-a-lysophosphatidylcholine-hydrolase-and-interacts-with-lipid-droplets-through-its-catalytic-domain
#20
Christoph Heier, Benedikt Kien, Feifei Huang, Thomas O Eichmann, Hao Xie, Rudolf Zechner, Ping-An Chang
Mammalian patatin-like phospholipase domain containing proteins (PNPLAs) are lipid metabolizing enzymes with essential roles in energy metabolism, skin barrier development, and brain function. A detailed annotation of enzymatic activities and structure-function relationships remains an important prerequisite to understand PNPLA functions in (patho-)physiology, for example, in disorders such as neutral lipid storage disease, non-alcoholic fatty liver disease, and neurodegenerative syndromes. In this study, we characterized the structural features controlling the subcellular localization and enzymatic activity of PNPLA7, a poorly annotated phospholipase linked to insulin signaling and energy metabolism...
September 7, 2017: Journal of Biological Chemistry
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