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Phospholipase c

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https://www.readbyqxmd.com/read/27932551/antigen-unmasking-enhances-visualization-efficacy-of-the-oocyte-activation-factor-phospholipase-c-zeta-in-mammalian-sperm
#1
Junaid Kashir, Luke Buntwal, Michail Nomikos, Brian L Calver, Panagiotis Stamatiadis, Peter Ashley, Vyronia Vassilakopoulou, David Sanders, Paul Knaggs, Evangelia Livaniou, Adnan Bunkheila, Karl Swann, F Anthony Lai
STUDY QUESTION: Is it possible to improve clinical visualization of phospholipase C zeta (PLCζ) as a diagnostic marker of sperm oocyte activation capacity and male fertility? SUMMARY ANSWER: Poor PLCζ visualization efficacy using current protocols may be due to steric or conformational occlusion of native PLCζ, hindering antibody access, and is significantly enhanced using antigen unmasking/retrieval (AUM) protocols. WHAT IS KNOWN ALREADY: Mammalian oocyte activation is mediated via a series of intracellular calcium (Ca(2+)) oscillations induced by sperm-specific PLCζ...
December 8, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27928450/lipoprotein-associated-phospholipase-a2-myeloperoxidase-and-vascular-endothelial-growth-factor-predictors-of-high-vascular-risk-in-respiratory-bacterial-infections
#2
Seri A, Marta Ds, Madalan A, Popescu M, Tiglea Ai, Moldoveanu E
Objective. Respiratory bacterial infections are associated with important coagulation disturbances that amplify the pulmonary lesions and determine a more severe course of the disease. The aim of our study was to investigate the correlation between the evolution of the general clinical parameters and the occurrence of thrombotic events on one side, and plasma levels of selected proteins involved in inflammation and coagulation on the other side, with the intent to establish and to validate a laboratory test panel for the assessment of the vascular risk in patients with bacterial respiratory infections...
October 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27920205/molecular-determinants-of-the-sensitivity-to-gq-11-phospholipase-c-dependent-gating-gd3-potentiation-and-ca2-permeability-in-the-transient-receptor-potential-canonical-type-5-trpc5-channel
#3
Xingjuan Chen, Wennan Li, Ashley M Riley, Mario Soliman, Saikat Chakraborty, Christopher W Stamatkin, Alexander G Obukhov
Transient Receptor Potential Canonical type 5 (TRPC5) is a Ca(2+) permeable cation channel that is highly expressed in the brain and is implicated in motor coordination, innate fear behavior, and seizure-genesis. The channel is activated by a signal downstream of the G-protein-coupled receptor (GPCR)-Gq/11-phospholipase C (PLC) pathway. In this study, we aimed to identify the molecular mechanisms involved in regulating TRPC5 activity. We report that R593, a residue located in the E4 loop near the TRPC5's extracellular Gd(3+)-binding site, is critical for conferring the sensitivity to GPCR-Gq/11-PLC-dependent gating on TRPC5...
December 5, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27918459/synthesis-antiphospholipase-a%C3%A2-antiprotease-antibacterial-evaluation-and-molecular-docking-analysis-of-certain-novel-hydrazones
#4
Nahed N E El-Sayed, Ahmed M Alafeefy, Mohammed A Bakht, Vijay H Masand, Ali Aldalbahi, Nan Chen, Chunhai Fan, Abir Ben Bacha
Some novel hydrazone derivatives 6a-o were synthesized from the key intermediate 4-Chloro-N-(2-hydrazinocarbonyl-phenyl)-benzamide 5 and characterized using IR, ¹H-NMR, (13)C-NMR, mass spectroscopy and elemental analysis. The inhibitory potential against two secretory phospholipase A₂ (sPLA₂), three protease enzymes and eleven bacterial strains were evaluated. The results revealed that all compounds showed preferential inhibition towards hGIIA isoform of sPLA₂ rather than DrG-IB with compounds 6l and 6e being the most active...
December 2, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27914990/presence-of-melanopsin-in-human-crystalline-lens-epithelial-cells-and-its-role-in-melatonin-synthesis
#5
Hanan Awad Alkozi, Xiaoyu Wang, Maria Jesus Perez de Lara, Jesus Pintor
Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the concentration of melatonin and its synthesising enzyme AANAT proved a clear link between melanopsin activation and the suppression of melatonin synthesis. Melanopsin sensitivity to specific wavelength (465-480 nm, blue) was confirmed after making temporal studies incubating lens epithelial cells under light, red, green, blue and total darkness for 2, 4, 8, 12 h and analysing the concentration of both melatonin and its synthesising enzyme AANAT, discovering that melatonin levels after submitting cells to total darkness are significantly higher to ones submitted to white or specifically blue light (***p < 0...
November 30, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27913876/atherosclerosis-assessment-and-rheumatoid-arthritis
#6
C Bes, S Gürel, G Buğdaycı, O Dikbaş, M Soy
BACKGROUND AND AIM: Rheumatoid arthritis is associated with increased morbidity and mortality due to atherosclerotic cardiovascular diseases. Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an enzyme of phospholipase A2; it plays an important role in inflammation and atherosclerosis. Herein we aimed to investigate whether Lp-PLA2 activity is associated with atherosclerosis in patients with rheumatoid arthritis and compare the Lp-PLA2 activity with carotid intima media thickness (CIMT)...
December 2, 2016: Zeitschrift Für Rheumatologie
https://www.readbyqxmd.com/read/27913297/phospholipase-c%C3%AE-1-suppresses-foreign-body-giant-cell-formation-by-maintaining-runx1-expression-in-macrophages
#7
Ye Seon Kim, Chang Youp Ok, Joon Seong Park, Ha Young Lee, Yoe-Sik Bae
Foreign body giant cell (FBGC) formation is associated with the inflammatory response following material implantation. However, the intracellular signaling events that regulate the process remain unclear. Here, we investigated the potential role of phospholipase C (PLC)γ1, a crucial enzyme required for growth factor-induced signaling, on FBGC formation. Knock-down of PLCγ1 using shRNA induced FBGC formation accompanied by increased expression of cathepsin K, DC-STAMP and CD36. Re-addition of PLCγ1 decreased FBGC formation...
November 30, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27913208/the-functions-of-store-operated-calcium-channels
#8
REVIEW
James W Putney, Natacha Steinckwich-Besançon, Takuro Numaga-Tomita, Felicity M Davis, Pooja N Desai, Diane M D'Agostin, Shilan Wu, Gary S Bird
Store-operated calcium channels provide calcium signals to the cytoplasm of a wide variety of cell types. The basic components of this signaling mechanism include a mechanism for discharging Ca(2+) stores (commonly but not exclusively phospholipase C and inositol 1,4,5-trisphosphate), a sensor in the endoplasmic reticulum that also serves as an activator of the plasma membrane channel (STIM1 and STIM2), and the store-operated channel (Orai1, 2 or 3). The advent of mice genetically altered to reduce store-operated calcium entry globally or in specific cell types has provided important tools to understand the functions of these widely encountered channels in specific and clinically important physiological systems...
November 29, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27913142/candida-species-from-oral-cavity-of-hiv-infected-children-exhibit-reduced-virulence-factors-in-the-haart-era
#9
Maristela Barbosa Portela, Elaine Lima de Amorim, Adrielle Mangabeira Santos, José Alexandre da Rocha Curvelo, Karol de Oliveira Martins, Cauli Lima Capillé, Rosangela Maria de Araújo Soares, Gloria Fernanda Barbosa de Araújo Castro
This study aimed to assess, in vitro, the biofilm viability and the phospholipase and protease production of Candida spp. from the saliva of HIV infected children and healthy controls, and to correlate the results with the use of medical data. A total of 79 isolates were analyzed: 48 Candida albicans isolates (33/15) and 20 Candida parapsilosis sensu lato complex isolates (12/8) (from HIV/control patients, respectively), and 8 Candida krusei, 1 Candida tropicalis, 1 Candida dubliniensis and 1 Candida guilliermondii from HIV patients...
November 29, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27911900/clinical-and-pharmacological-investigation-of-myotoxicity-in-sri-lankan-russell-s-viper-daboia-russelii-envenoming
#10
Anjana Silva, Christopher Johnston, Sanjaya Kuruppu, Daniela Kneisz, Kalana Maduwage, Oded Kleifeld, A Ian Smith, Sisira Siribaddana, Nicholas A Buckley, Wayne C Hodgson, Geoffrey K Isbister
BACKGROUND: Sri Lankan Russell's viper (Daboia russelii) envenoming is reported to cause myotoxicity and neurotoxicity, which are different to the effects of envenoming by most other populations of Russell's vipers. This study aimed to investigate evidence of myotoxicity in Russell's viper envenoming, response to antivenom and the toxins responsible for myotoxicity. METHODOLOGY AND FINDINGS: Clinical features of myotoxicity were assessed in authenticated Russell's viper bite patients admitted to a Sri Lankan teaching hospital...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27911408/defining-substrate-specificities-for-lipase-and-phospholipase-candidates
#11
Diana X Sahonero-Canavesi, Maritza Zavaleta-Pastor, Lourdes Martínez-Aguilar, Isabel M López-Lara, Otto Geiger
Microorganisms produce a wide spectrum of (phospho)lipases that are secreted in order to make external substrates available for the organism. Alternatively, other (phospho)lipases may be physically associated with the producing organism causing a turnover of intrinsic lipids and frequently giving rise to a remodeling of the cellular membranes. Although potential (phospho)lipases can be predicted with a number of algorithms when the gene/protein sequence is available, experimental proof of the enzyme activities, substrate specificities, and potential physiological functions has frequently not been obtained...
November 23, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909057/structure-of-the-tir-domain-of-bcap-which-links-phosphoinositide-metabolism-with-the-negative-regulation-of-the-tlr-signalosome
#12
Samer Halabi, Eiki Sekine, Brett Verstack, Nicholas J Gay, Martin C Moncrieffe
Ligand binding to Toll-like receptors (TLRs) results in dimerization of their cytosolic TIR domains and recruitment of post-receptor signal transducers into a complex signalosome. TLR activation leads to the production of transcription factors, pro-inflammatory molecules and the activation of Phosphoinositide 3-kinases (PI3K) in a process that requires the multi-modular B-cell Adaptor for Phosphoinositide 3-kinase (BCAP). BCAP has a sequence previously proposed as a "cryptic" TIR domain. Here, we present the structure of the N-terminal region of human BCAP and show that it possesses a canonical TIR-fold...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27905898/physical-activity-and-sedentary-behavior-can-modulate-the-effect-of-the-pnpla3-variant-on-childhood-nafld-a-case-control-study-in-a-chinese-population
#13
Shuo Wang, Jieyun Song, Xiaorui Shang, Nitesh Chawla, Yide Yang, Xiangrui Meng, Haijun Wang, Jun Ma
BACKGROUND: The patatin like phospholipase containing domain 3 gene (PNPLA3) rs738409 C > G polymorphism, one of the most important gene polymorphisms involved in hepatic steatosis, has been reported to interact with different nutrients and dietary patterns on Non-Alcoholic Fatty Liver Disease (NAFLD), but no studies have focused on its interaction with physical activity or sedentary behavior. Therefore, this study aims at determining whether physical activity or sedentary behavior could modulate the effect of the PNPLA3 variant on childhood NAFLD...
December 1, 2016: BMC Medical Genetics
https://www.readbyqxmd.com/read/27899995/systematic-tracking-of-disrupted-modules-identifies-significant-genes-and-pathways-in-hepatocellular-carcinoma
#14
Meng-Hui Zhang, Qin-Hai Shen, Zhao-Min Qin, Qiao-Ling Wang, Xi Chen
The objective of the present study is to identify significant genes and pathways associated with hepatocellular carcinoma (HCC) by systematically tracking the dysregulated modules of re-weighted protein-protein interaction (PPI) networks. Firstly, normal and HCC PPI networks were inferred and re-weighted based on Pearson correlation coefficient. Next, modules in the PPI networks were explored by a clique-merging algorithm, and disrupted modules were identified utilizing a maximum weight bipartite matching in non-increasing order...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895625/evaluation-of-virulence-factors-in-vitro-resistance-to-osmotic-stress-and-antifungal-susceptibility-of-candida-tropicalis-isolated-from-the-coastal-environment-of-northeast-brazil
#15
Diana L Zuza-Alves, Sayama S T Q de Medeiros, Luanda B F C de Souza, Walicyranison P Silva-Rocha, Elaine C Francisco, Maria C B de Araújo, Reginaldo G Lima-Neto, Rejane P Neves, Analy S de Azevedo Melo, Guilherme M Chaves
Several studies have been developed regarding human health risks associated with the recreational use of beaches contaminated with domestic sewage. These wastes contain various micro-organisms, including Candida tropicalis. In this context, the objective of this study was to characterize C. tropicalis isolates from the sandy beach of Ponta Negra, Natal, Rio Grande do Norte, Brazil, regarding the expression of in vitro virulence factors, adaptation to osmotic stress and susceptibility to antifungal drugs. We analyzed 62 environmental isolates and observed a great variation among them for the various virulence factors evaluated...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27894895/molecular-characterization-and-expression-analysis-of-olive-flounder-paralichthys-olivaceus-phospholipase-c-gamma-1-and-gamma-2
#16
Soo Ji Woo, Hyae In Jo, Hyung Ho Lee, Joon Ki Chung
Phospholipase C gamma 1 and gamma 2 (PLCG1 and PLCG2) are influential in modulating Ca(2+) and diacylglycerol, second messengers involved in tyrosine kinase-dependent signaling, including growth factor activation. Here, we used RACE (rapid amplification of cDNA ends) to clone cDNA encoding PLCG1 (PoPLCG1) and PLCG2 (PoPLCG2) in the olive flounder (Paralichthys olivaceus). The respective 1313 and 1249 amino acid sequences share high identity with human PLCG1 and PLCG2, and contain the following domains: pleckstrin homology (PH), EF-hand, catalytic X and Y, Src homology 2 (SH2), Src homology 3 (SH3), and C2...
November 25, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27894086/the-anti-tumor-drug-2-hydroxyoleic-acid-minerval-stimulates-signaling-and-retrograde-transport
#17
Maria L Torgersen, Tove Irene Klokk, Simona Kavaliauskiene, Christian Klose, Kai Simons, Tore Skotland, Kirsten Sandvig
2-hydroxyoleic acid (OHOA, Minerval®) is an example of a substance used for membrane lipid therapy, where the cellular membranes rather than specific proteins constitute the therapeutical target. OHOA is thought to mediate its anti-tumor effect by affecting the biophysical properties of membranes, which leads to altered recruitment and activation of amphitropic proteins, altered cellular signaling, and eventual cell death. Little is known about the initial signaling events upon treatment with OHOA, and whether the altered membrane properties would have any impact on the dynamic intracellular transport system...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27888371/protective-effect-of-the-sulfated-agaran-isolated-from-the-red-seaweed-laurencia-aldingensis-against-toxic-effects-of-the-venom-of-the-snake-lachesis-muta
#18
Ana Cláudia Rodrigues da Silva, Luciana Garcia Ferreira, Maria Eugênia Rabello Duarte, Mutue Toyota Fujii, Eladio Flores Sanchez, Miguel Daniel Noseda, André Lopes Fuly
Snakebite is a serious occupational hazard affecting mainly rural populations of tropical and subtropical developing countries. Lachesis muta (Bushmaster) bites are extremely serious but are rarely reported in the literature. Bushmaster envenomings are characterized by intense local pain, edema, neurotoxicity, hypotension, local hemorrhage, and dramatic systemic alterations. Antivenom treatment has regularly been used for more than a century; however, it fails to neutralize local tissue damage and hemorrhage, leading to morbidity or disabilities in victims...
November 25, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27888240/topological-organisation-of-the-phosphatidylinositol-4-5-bisphosphate-phospholipase-c-resynthesis-cycle-pitps-bridge-the-er-pm-gap
#19
REVIEW
Shamshad Cockcroft, Padinjat Raghu
Phospholipase C (PLC) is a receptor-regulated enzyme that hydrolyses phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at the plasma membrane (PM) triggering three biochemical consequences, the generation of soluble inositol 1,4,5-trisphosphate (IP3), membrane-associated diacylglycerol (DG) and the consumption of PM PI(4,5)P2 Each of these three signals triggers multiple molecular processes impacting key cellular properties. The activation of PLC also triggers a sequence of biochemical reactions, collectively referred to as the PI(4,5)P2 cycle that culminates in the resynthesis of this lipid...
December 1, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27882139/the-association-between-the-levels-of-crp-il-10-pla2-fbg-and-prognosis-in-traumatic-fracture-of-lower-limb
#20
Jing Jiao, Jun-Wen Wang, Fei Xiao, Yu-Cheng Huang
The aim of the present study was to examine changes of sera levels of C-reactive protein (CRP), interleukin-10 (IL-10), phospholipase A2 (PLA2) and fibrinogen β polypeptide chain gene (Fbg) in patients with traumatic fracture of lower limb, and to evaluate their association with prognosis. The changes in sera levels of CRP, IL-10, PLA2 and Fbg were observed at the time of injury, 24 h, and 5 and 7 days after surgery in 90 patients with traumatic fracture of lower limb. In addition, 50 cases, who presented for health examination, were included as the normal controls...
November 2016: Experimental and Therapeutic Medicine
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