keyword
MENU ▼
Read by QxMD icon Read
search

Phospholipase c

keyword
https://www.readbyqxmd.com/read/29792176/anti-neutrophil-cytoplasmic-antibody-associated-glomerulonephritis-with-detection-of-myeloperoxidase-and-phospholipase-a-2-receptor-in-membranous-nephropathy-lesions-report-of-two-patients-with-microscopic-polyangiitis
#1
Kenta Tominaga, Takahiro Uchida, Toshihiko Imakiire, Kenji Itoh, Hideyuki Shimazaki, Kuniaki Nakanishi, Hiroo Kumagai, Naoki Oshima
BACKGROUND: Podocyte phospholipase A2 receptor (PLA2 R) is a major target antigen in idiopathic adult membranous nephropathy (MN). Histological PLA2 R staining in the renal tissue has proven to be useful for the detection of idiopathic MN. However, glomerular PLA2 R deposits have also been recently observed in several patients with secondary MN, such as hepatitis B virus-associated, hepatitis C virus-associated, and neoplasm-associated MN. Certain inflammatory environments have been suggested to lead to abnormal expression of PLA2 R epitopes, with the resulting production of PLA2 R autoantibodies...
May 23, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/29791754/atp-triggers-a-robust-intracellular-ca-2-mediated-signalling-pathway-in-human-synovial-fibroblasts
#2
Kondo C, R B Clark, T Y Kim, Belke D, Banderali U, R T Szerencsei, A H Jalloul, P P M Schnetkamp, Spitzer Kw, Giles Wr
NEW FINDINGS: What is the central question of this study? In human articular joints, the synovial fibroblast HSF cell population has essential functions including secretion of lubricants. We address some remaining questions concerning activation-secretion coupling by characterizing the main [Ca2+ ]i signalling pathways activated by ATP. What is the main finding and its importance? In HSF ATP acts through a linked G-protein (Gq ) and phospholipase C signalling mechanisms to produce IP-3 which markedly enhances release of Ca2+ from the endoplasmic reticulum...
May 23, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29790164/purinergic-receptor-stimulation-induces-calcium-oscillations-and-smooth-muscle-contraction-in-small-pulmonary-veins
#3
Mauricio Henriquez, Marcelo Fonseca, Jose F Perez-Zoghbi
KEY POINTS: We investigated the excitation-contraction coupling mechanisms in small pulmonary veins (SPVs) in rat precision-cut lung slices. We found that SPVs contract strongly and reversibly in response to extracellular ATP and other vasoconstrictors, including angiotensin-II and endothelin-1. ATP-induced vasoconstriction in SPVs was associated with the stimulation of purinergic P2Y2 receptors in vascular smooth muscle cell, activation of phospholipase C-β and the generation of intracellular Ca2+ oscillations mediated by cyclic Ca2+ release events via the inositol 1,4,5-trisphosphate receptor...
May 23, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29789355/hdl-functionality-and-cardiovascular-outcome-among-non-dialysis-chronic-kidney-disease-patients
#4
Kathrin Untersteller, Sabine Meissl, Markus Trieb, Insa Emrich, Adam Zawada, Michael Holzer, Eva Knuplez, Danilo Fliser, Gunnar Heine, Gunther Marsche
Cardiovascular disease remains the leading cause of morbidity and mortality in patients with chronic kidney disease (CKD). CKD profoundly affects high-density lipoprotein (HDL) composition and functionality, but if abnormal HDL independently contributes to cardiovascular events in CKD patients remains elusive. In the present study, we assessed whether compositional and functional properties of HDL predict cardiovascular outcome among 526 non-dialysis CKD patients who participate in the CARE FOR HOMe study. We measured HDL cholesterol, the content of HDL-associated pro-inflammatory serum amyloid A (SAA) and activities of the HDL enzymes paraoxonase and lipoprotein associated phospholipase A2 (Lp-PLA2)...
May 22, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29785630/calcium-channels-rho-kinase-protein-kinase-c-and-phospholipase-c-pathways-mediate-mercury-chloride-induced-myometrial-contractions-in-rats
#5
Swati Koli, Atul Prakash, Soumen Choudhury, Rajesh Mandil, Satish K Garg
Adverse effects of mercury on female reproduction are reported; however, its effect on myogenic activity of uterus and mechanism thereof is obscure. Present study was undertaken to unravel the mechanistic pathways of mercuric chloride (HgCl2 )-induced myometrial contraction in rats. Isometric tension in myometrial strips of rats following in vitro exposure to HgCl2 was recorded using data acquisition system-based physiograph. HgCl2 produced concentration-dependent (10 nM-100 μM) uterotonic effect which was significantly (p < 0...
May 21, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29779871/non-canonical-phototransduction-mediates-synchronization-of-the-drosophila-melanogaster-circadian-clock-and-retinal-light-responses
#6
Maite Ogueta, Roger C Hardie, Ralf Stanewsky
The daily light-dark cycles represent a key signal for synchronizing circadian clocks. Both insects and mammals possess dedicated "circadian" photoreceptors but also utilize the visual system for clock resetting. In Drosophila, circadian clock resetting is achieved by the blue-light photoreceptor cryptochrome (CRY), which is expressed within subsets of the brain clock neurons. In addition, rhodopsin-expressing photoreceptor cells contribute to light synchronization. Light resets the molecular clock by CRY-dependent degradation of the clock protein Timeless (TIM), although in specific subsets of key circadian pacemaker neurons, including the small ventral lateral neurons (s-LNvs), TIM and Period (PER) oscillations can be synchronized by light independent of CRY and canonical visual Rhodopsin phototransduction...
April 30, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29779757/neutral-lipid-storage-disease-with-myopathy-further-phenotypic-characterization-of-a-rare-pnpla2-variant
#7
Caitlin S Latimer, Jennifer Schleit, Adam Reynolds, Desiree A Marshall, Benjamin Podemski, Leo H Wang, Luis F Gonzalez-Cuyar
Neutral lipid storage disease with myopathy is a rare disorder of lipid metabolism caused by variants in the Patatin-Like Phospholipase Domain Containing 2 (PNPLA2) gene. Diagnosis is often delayed due to variable presentations, which is of concern due to increased risk of cardiomyopathy. Better phenotype-genotype characterization is necessary to improve speed and accuracy of diagnosis. Here, we describe a 32-year-old woman of Hmong descent with progressive muscle pain and weakness who had a muscle biopsy with characteristic features of a lipid storage myopathy...
April 19, 2018: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/29778633/comparative-analysis-of-proteinase-phospholipase-hydrophobicity-and-biofilm-forming-ability-in-candida-species-isolated-from-clinical-specimens
#8
S Dabiri, M Shams-Ghahfarokhi, M Razzaghi-Abyaneh
Candida species are the commensal organisms of human and animal mucosa that cause a wide range of debilitating diseases in immunocompromised patients and other susceptible individuals. The present study aimed to investigate the ability of clinical isolates of various Candida species to produce proteinase and phospholipase, hydrophobicity and biofilm forming ability that assumed to have a vital role in Candida pathogenicity. Eighty-four Candida strains belonged to Candida albicans (44.1%), C. glabrata (5.9%), C...
May 16, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29770198/recent-advances-in-managing-understanding-meningioma
#9
REVIEW
Nawal Shaikh, Karan Dixit, Jeffrey Raizer
Meningiomas are the most common adult primary intracranial tumor. Despite their higher incidence, there have not-until recently-been as many advances in understanding and managing meningiomas. Thus far, two broad classes of meningiomas have emerged on the basis of their mutational profile: those driven by neurofibromatosis 2 (NF2) inactivation and those with non-NF2 driver gene alterations, such as mammalian target of rapamycin and Hedgehog, Wingless/b-catenin, Notch, transforming growth factor-b receptor, mitogen-activated protein kinase, and phospholipase C pathway alterations...
2018: F1000Research
https://www.readbyqxmd.com/read/29765452/effects-and-potential-mechanism-of-atorvastatin-treatment-on-lp-pla2-in-rats-with-dyslipidemia
#10
Dongdan Zheng, Anping Cai, Rulin Xu, Zhuocheng Mai, Yingling Zhou, Fanfang Zeng, Liwen Li, Weiyi Mai
Introduction: The effects of statins on lipoprotein-associated phospholipase A2 (Lp-PLA2) are controversial, and the present study aimed to investigate whether atorvastatin could reduce Lp-PLA2 in rats with dyslipidemia. Material and methods: A high-fat and high-cholesterol diet was prescribed to produce a dyslipidemia model. Thereafter, low-dose atorvastatin (5 mg/kg/day), high-dose atorvastatin (20 mg/kg/day) or saline (without-treatment group) was prescribed for 14 days...
April 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29752814/valproic-acid-inhibits-atp-triggered-ca-2-release-via-a-p38-dependent-mechanism-in-bend-3-endothelial-cells
#11
Iat-Lon Leong, Tien-Yao Tsai, Kar-Lok Wong, Lian-Ru Shiao, Ka-Shun Cheng, Paul Chan, Yuk-Man Leung
Valproic acid (VA) is currently used to treat epilepsy and bipolar disorder. It has also been demonstrated to promote neuroprotection and neurogenesis. Although beneficial actions of VA on brain blood vessels have also been demonstrated, the effects of VA on brain endothelial cell (EC) Ca2+ signaling is hitherto unreported. In this report we examined the effects of VA on agonist-triggered Ca2+ signaling in mouse cortical bEND.3 EC. While VA (100 μM) did not cause an acute inhibition of ATP-triggered Ca2+ signaling, a 30-min VA treatment strongly suppressed ATP-triggered intracellular Ca2+ release; however, such treatment did not affect Ca2+ release triggered by cyclopiazonic acid, an inhibitor of SERCA Ca2+ pump, suggesting there was no reduction in Ca2+ store size...
May 12, 2018: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/29752494/morphological-observation-and-comparative-transcriptomic-analysis-of-clostridium-perfringens-biofilm-and-planktonic-cells
#12
Xiaofen Zhang, Yuhua Ma, Guisheng Ye
Bacterial biofilms can enhance survival in adverse environments and promote infection. However, little is known about biofilm formation by Clostridium perfringens. To better characterize this process, we used SEM to observe the surfaces of C. perfringens biofilms after 12, 24, 48, and 72 h of incubation. Biofilm cells appeared to be encased in a dense matrix material, and the total biomass of the biofilm increased with incubation time. To gain insight into the differentially expressed genes (DEGs) between biofilm and planktonic cells, we carried out comparative transcriptomic analysis using RNA sequencing...
May 11, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29750435/signaling-pathways-involved-in-human-sperm-hyperactivated-motility-stimulated-by-zn-2
#13
Deborah Allouche-Fitoussi, Danit Bakhshi, Haim Breitbart
To fertilize the egg, sperm cells must reside in the female reproductive tract for several hours during which they undergo chemical and motility changes collectively called capacitation. During capacitation, the sperm develop a unique type of motility known as hyper-activated motility (HAM). The semen contains Zn2+ in millimolar concentrations, whereas in the female reproductive tract the concentration is around 1 µM. In this study, we characterize the role of Zn2+ in human sperm capacitation focusing on its effect on HAM...
May 11, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29749493/a-novel-pnpla6-compound-heterozygous-mutation-identified-in-a-chinese-patient-with-boucher%C3%A2-neuh%C3%A3-user-syndrome
#14
Ruizhi Zheng, Yaguang Zhao, Jiayu Wu, Yuanmei Wang, Jian-Ling Liu, Zhi-Ling Zhou, Xiao-Tao Zhou, Dan-Na Chen, Wei-Hua Liao, Jia-Da Li
The combination of cerebellar degeneration, hypogonadotropic hypogonadism and chorioretinal dystrophy defines Boucher‑Neuhäuser syndrome (BNS), which has been associated with autosomal‑recessive mutations in the patatin‑like phospholipase domain containing 6 (PNPLA6) gene. However, no BNS cases have been reported in mainland China. In the present study, to the best of the authors' knowledge, the first patient with BNS was identified in China. A 39‑year‑old male was first diagnosed with hypogonadotropic hypogonadism...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29737567/impact-of-a-non-synonymous-q281r-polymorphism-on-structure-of-human-lipoprotein-associated-phospholipase-a-2-lp-pla-2
#15
Arun B Gurung, Atanu Bhattacharjee
Non-synonymous single nucleotide polymorphisms (nsSNPs) are genetic variations at single base resulting in an amino acid change which have been associated with various complex human diseases. The human Lipoprotein-associated phospholipase A2 (Lp-PLA2 ) gene harbours a rare Q281R polymorphism which was previously reported to cause loss of enzymatic function. Lp-PLA2 is an important enzyme which catalyzes the hydrolysis of polar phospholipids releasing pro-atherogenic and pro-inflammatory mediators involved in the pathogenesis of atherosclerosis...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29734363/%C3%AE-arrestin1-and-2-differentially-regulate-pacap-induced-pac1-receptor-signaling-and-trafficking
#16
Yusuke Shintani, Atsuko Hayata-Takano, Keita Moriguchi, Takanobu Nakazawa, Yukio Ago, Atsushi Kasai, Kaoru Seiriki, Norihito Shintani, Hitoshi Hashimoto
A pituitary adenylate cyclase-activating polypeptide (PACAP)-specific receptor, PAC1R, is coupled with multiple signal transduction pathways including stimulation of adenylate cyclase, phospholipase C and extracellular-signal regulated kinase (ERK)1/2. PAC1R has been shown to exert its long-lasting and potent signals via β-arrestin1 and β-arrestin2. However, the precise roles of the two β-arrestin isoforms in PACAP-PAC1R signaling remain unclear. Here we examined the interaction between the two β-arrestin isoforms and PAC1R, β-arrestin-dependent PAC1R subcellular localization and ERK1/2 activation...
2018: PloS One
https://www.readbyqxmd.com/read/29734114/the-early-signaling-pathway-of-live-yeast-cell-derivative-in-thp-1-monocytes
#17
Eric I Gruenstein, Donna J Schlemm, Mridula Bethi, Stephen J Keller
Live yeast cell derivative (LYCD) is a medicinal yeast extract that has been used in the treatment of burns, wounds and hemorrhoids for over 70 years. It has been shown to enhance the closure of skin wounds in diabetic mice by increasing inflammation, angiogenesis, formation of granulation tissue and epithelial migration. An active fraction of LYCD has been identified as a mixture of peptides ranging in size from 5 kDA to 17 kDA. Despite its widespread use over many years, understanding of the mechanism by which LYCD acts to effect tissue repair responses is very limited...
April 22, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29731504/application-of-auxin-inducible-degron-technology-to-mouse-oocyte-activation-with-plc%C3%AE
#18
Kento Miura, Shogo Matoba, Narumi Ogonuki, Takafumi Namiki, Junya Ito, Naomi Kashiwazaki, Atsuo Ogura
In mammals, spermatozoa activate oocytes by triggering a series of intracellular Ca2+ oscillations with phospholipase C zeta (PLCζ), a sperm-borne oocyte-activating factor. Because the introduction of PLCζ alone can induce oocyte activation, it might be a promising reagent for assisted reproductive technologies. To test this possibility, we injected human PLCζ (hPLCζ) mRNA into mouse oocytes at different concentrations. We observed the oocyte activation and subsequent embryonic development. Efficient oocyte activation and embryonic development to the blastocyst stage was achieved only with a limited range of mRNA concentrations (0...
May 5, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29730398/capa-periviscerokinin-mediated-activation-of-mapk-erk-signaling-through-gq-plc-pkc-dependent-cascade-and-reciprocal-erk-activation-dependent-internalized-kinetics-of-bom-capa-pvk-receptor-2
#19
Zhangfei Shen, Xiaoyuan Yang, Yu Chen, Liangen Shi
Bombyx mori neuropeptide G protein-coupled receptor (BNGR)-A27 is a specific receptor for B. mori capability (CAPA) periviscerokinin (PVK), that is, Bom-CAPA-PVK receptor 2. Upon stimulation of Bom-CAPA-PVK-1 or -PVK-2, Bom-CAPA-PVK receptor 2 significantly increases cAMP-response element-controlled luciferase activity and Ca2+ mobilization in a Gq inhibitor-sensitive manner. However, the underlying mechanism(s) for CAPA/CAPA receptor system mediation of extracellular signal-regulated kinases1/2 (ERK1/2) activation remains to be explained further...
May 3, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29729992/proteomics-functional-characterization-and-antivenom-neutralization-of-the-venom-of-pakistani-russell-s-viper-daboia-russelii-from-the-wild
#20
Tasnim Faisal, Kae Yi Tan, Si Mui Sim, Naeem Quraishi, Nget Hong Tan, Choo Hock Tan
The venom proteome of wild Pakistani Russell's viper (Daboia russelii) was investigated through nano-ESI-LCMS/MS of the reverse-phase HPLC fractions. A total of 54 venom proteins were identified and clustered into 11 protein families. Phospholipase A2 (PLA2 , 63.8%) and Kunitz-type serine protease inhibitor (KSPI, 16.0%) were most abundant, followed by snake venom serine protease (SVSP, 5.5%, mainly Factor V activating enzyme), vascular endothelial growth factor (VEGF, 4.3%), snake venom metalloprotease (SVMP, 2...
May 3, 2018: Journal of Proteomics
keyword
keyword
39431
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"