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Nuclear lipidomics

Ljubica Tasic, João G M Pontes, Michelle S Carvalho, Guilherme Cruz, Carolines Dal Mas, Sumit Sethi, Mariana Pedrini, Lucas B Rizzo, Maiara Zeni-Graiff, Elson Asevedo, Acioly L T Lacerda, Rodrigo A Bressan, Ronei Jesus Poppi, Elisa Brietzke, Mirian A F Hayashi
Using (1)H NMR-based metabolomics in association to chemometrics analysis, we analyzed here the metabolic differences between schizophrenia patients (SCZ) compared to healthy controls (HCs). HCs and SCZ patients underwent clinical interview using the Structured Clinical Interview for DSM Disorders (SCID). SCZ patients were further assessed by Positive and Negative Syndrome Scale (PANSS), Calgary Depression Scale, Global Assessment of Functioning Scale (GAF), and Clinical Global Impressions Scale (CGI). Using the principal component analysis (PCA) and supervised partial least-squares discriminate analysis (PLS-DA) in obtained NMR data, a clear group separation between HCs and SCZ patients was achieved...
December 28, 2016: Schizophrenia Research
He Tian, Sin Man Lam, Guanghou Shui
Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in biological samples. As complements to transcriptomics and proteomics, it has played pivotal roles in agricultural and food science research. In this review, we discuss the capacities of NMR, GC/LC-MS in the acquisition of plant metabolome, and address the potential promise and diverse applications of metabolomics, particularly lipidomics, to investigate the responses of Arabidopsis thaliana, a primary plant model for agricultural research, to environmental stressors including heat, freezing, drought, and salinity...
November 17, 2016: International Journal of Molecular Sciences
B Stratmann, Y Krepak, E Schiffer, I Jarick, M Hauber, Y H Lee-Barkey, M Fischer, D Tschoepe
Implantation of a duodenal-jejunal endoluminal bypass liner (DJBL) has shown to induce weight loss and to improve metabolic parameters. DJBL is a reversible endoduodenal sleeve mimicking duodenal bypass while lacking risks and limitations of bariatric surgery.Effects on metabolic control, body mass parameters, appetite regulation, glucose tolerance, organ health, and lipid profile were determined in 16 morbidly overweight patients with type 2 diabetes mellitus. In addition, relevant hormones (leptin, ghrelin, gastric inhibitory peptide, glucagon-like peptide, and insulin) were measured by enzyme-linked immunosorbent assay (ELISA) and chemiluminescent microparticle immunoassay (CMIA) at 0, 1, 32, and 52 weeks post-implant following a mixed meal tolerance test...
September 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
Yang Yu, Matej Skočaj, Mateja Erdani Kreft, Nataša Resnik, Peter Veranič, Pietro Franceschi, Kristina Sepčić, Graziano Guella
Comparative lipidomic studies were performed across the RT4 versus T24 urothelial cancer cell lines, as models for noninvasive urothelial papilloma cells (with a relatively high level of differentiation) and invasive urothelial carcinoma cells (with low level of differentiation), respectively. The aim was to investigate the differences in lipid profile associated with different levels of urothelial cancer cell invasiveness. The cellular lipidomes were characterized using our previously developed joint methodology of liquid chromatography-mass spectrometry and high-resolution nuclear magnetic resonance, which included analysis of the phospholipids and ceramide-based glycosphingolipids...
October 18, 2016: Molecular BioSystems
Martin Flück, Severin Ruoss, Christoph B Möhl, Paola Valdivieso, Mario C Benn, Brigitte von Rechenberg, Endre Laczko, Junmin Hu, Karl Wieser, Dominik C Meyer, Christian Gerber
Reversal of fatty infiltration of pennate rotator cuff muscle after tendon release is hitherto impossible. The administration of nandrolone starting at the time of tendon release prevents the increase in fat content, but does not revert established fatty infiltration. We hypothesised that tendon release and myotendinous retraction cause alterations in lipid related gene expression leading to fatty muscle infiltration, which can be suppressed by nandrolone through its genomic actions if applied immediately after tendon release...
January 2017: Journal of Steroid Biochemistry and Molecular Biology
Rona Aviram, Gal Manella, Naama Kopelman, Adi Neufeld-Cohen, Ziv Zwighaft, Meytar Elimelech, Yaarit Adamovich, Marina Golik, Chunyan Wang, Xianlin Han, Gad Asher
Cells have evolved mechanisms to handle incompatible processes through temporal organization by circadian clocks and by spatial compartmentalization within organelles defined by lipid bilayers. Recent advances in lipidomics have led to identification of plentiful lipid species, yet our knowledge regarding their spatiotemporal organization is lagging behind. In this study, we quantitatively characterized the nuclear and mitochondrial lipidome in mouse liver throughout the day, upon different feeding regimens, and in clock-disrupted mice...
May 19, 2016: Molecular Cell
Margery A Connelly, Irina Shalaurova, James D Otvos
Great advances are being made at the mechanistic level in the understanding of the structural and functional diversity of high-density lipoprotein (HDL). HDL particle subspecies of different sizes are now known to differ in the protein and lipid cargo they transport, conferring on them the ability to perform different functions that in aggregate would be expected to provide protection against the development of atherosclerosis and its downstream clinical consequences. Exacerbating what is already a very complex system is the finding that inflammation, via alteration of the proteomic and lipidomic composition of HDL subspecies, can modulate at least some of their functional activities...
July 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
Wei Wei, Adam G Schwaid, Xueqian Wang, Xunde Wang, Shili Chen, Qian Chu, Alan Saghatelian, Yihong Wan
Nuclear receptors (NRs) are key regulators of gene expression and physiology. Nearly half of all human NRs lack endogenous ligands including estrogen-related receptor α (ERRα). ERRα has important roles in cancer, metabolism, and skeletal homeostasis. Affinity chromatography of tissue lipidomes with the ERRα ligand-binding domain (LBD) and subsequent transcriptional assays identified cholesterol as an endogenous ERRα agonist. Perturbation of cholesterol biosynthesis or inhibition of ERRα revealed the interdependence of cholesterol and ERRα...
March 8, 2016: Cell Metabolism
Zsuzsanna Ament, James A West, Elizabeth Stanley, Tom Ashmore, Lee D Roberts, Jayne Wright, Andrew W Nicholls, Julian L Griffin
The peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors that regulate cellular homoeostasis and metabolism. PPARs control the expression of genes involved in fatty-acid and lipid metabolism. Despite evidence showing beneficial effects of their activation in the treatment of metabolic diseases, particularly dyslipidaemias and type 2 diabetes, PPAR agonists have also been associated with a variety of side effects and adverse pathological changes. Agonists have been developed that simultaneously activate the three PPAR receptors (PPARα, γ and δ) in the hope that the beneficial effects can be harnessed while avoiding some of the negative side effects...
June 2016: Free Radical Biology & Medicine
Tomas Cajka, Oliver Fiehn
No abstract text is available yet for this article.
January 5, 2016: Analytical Chemistry
Nanami Senoo, Noriyuki Miyoshi, Naoko Goto-Inoue, Kimiko Minami, Ryoji Yoshimura, Akihito Morita, Naoki Sawada, Junichiro Matsuda, Yoshihiro Ogawa, Mitsutoshi Setou, Yasutomi Kamei, Shinji Miura
Exercise training influences phospholipid fatty acid composition in skeletal muscle and these changes are associated with physiological phenotypes; however, the molecular mechanism of this influence on compositional changes is poorly understood. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a nuclear receptor coactivator, promotes mitochondrial biogenesis, the fiber-type switch to oxidative fibers, and angiogenesis in skeletal muscle. Because exercise training induces these adaptations, together with increased PGC-1α, PGC-1α may contribute to the exercise-mediated change in phospholipid fatty acid composition...
December 2015: Journal of Lipid Research
Fabrice Tranchida, Laetitia Shintu, Zo Rakotoniaina, Léopold Tchiakpe, Valérie Deyris, Abel Hiol, Stefano Caldarelli
We explored, using nuclear magnetic resonance (NMR) metabolomics and fatty acids profiling, the effects of a common nutritional complement, Curcuma longa, at a nutritionally relevant dose with human use, administered in conjunction with an unbalanced diet. Indeed, traditional food supplements have been long used to counter metabolic impairments induced by unbalanced diets. Here, rats were fed either a standard diet, a high level of fructose and saturated fatty acid (HFS) diet, a diet common to western countries and that certainly contributes to the epidemic of insulin resistance (IR) syndrome, or a HFS diet with a Curcuma longa extract (1% of curcuminoids in the extract) for ten weeks...
2015: PloS One
Maryam Goudarzi, Waylon M Weber, Juijung Chung, Melanie Doyle-Eisele, Dunstana R Melo, Tytus D Mak, Steven J Strawn, David J Brenner, Raymond Guilmette, Albert J Fornace
Despite considerable research into the environmental risks and biological effects of exposure to external beam γ rays, incorporation of radionuclides has largely been understudied. This dosimetry and exposure risk assessment is challenging for first responders in the field during a nuclear or radiological event. Therefore, we have developed a workflow for assessing injury responses in easily obtainable biofluids, such as urine and serum, as the result of exposure to internal emitters cesium-137 ((137)Cs) and strontium-90 ((90)Sr) in mice...
September 4, 2015: Journal of Proteome Research
Antonio Checa, Carmen Bedia, Joaquim Jaumot
Lipids are a broad group of biomolecules involved in diverse critical biological roles such as cellular membrane structure, energy storage or cell signaling and homeostasis. Lipidomics is the -omics science that pursues the comprehensive characterization of lipids present in a biological sample. Different analytical strategies such as nuclear magnetic resonance or mass spectrometry with or without previous chromatographic separation are currently used to analyze the lipid composition of a sample. However, current analytical techniques provide a vast amount of data which complicates the interpretation of results without the use of advanced data analysis tools...
July 23, 2015: Analytica Chimica Acta
He Tian, Weiyu Wang, Nan Zheng, Jianbo Cheng, Songli Li, Yangdong Zhang, Jiaqi Wang
Controlling heat stress (HS) is a global challenge for the dairy industry. In this work, an integrated metabolomics and lipidomics approach using (1)H nuclear magnetic resonance (NMR) and ultra-fast LC-MS in combination with multivariate analyses was employed to investigate the discrimination of plasma metabolic profiles between HS-free and HS lactating dairy cows. Here we provide the information about the acquiring and processing of raw data obtained by (1)H NMR and LC-MS techniques. The data of present study are related to the research article "Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows" in the Journal of Proteomics (Tian et al...
September 2015: Data in Brief
Imo E Hoefer, Sabine Steffens, Mika Ala-Korpela, Magnus Bäck, Lina Badimon, Marie-Luce Bochaton-Piallat, Chantal M Boulanger, Giuseppina Caligiuri, Stefanie Dimmeler, Jesus Egido, Paul C Evans, Tomasz Guzik, Brenda R Kwak, Ulf Landmesser, Manuel Mayr, Claudia Monaco, Gerard Pasterkamp, Jose Tuñón, Christian Weber
Identification of subjects at increased risk for cardiovascular events plays a central role in the worldwide efforts to improve prevention, prediction, diagnosis, and prognosis of cardiovascular disease and to decrease the related costs. Despite their high predictive value on population level, traditional risk factors fail to fully predict individual risk. This position paper provides a summary of current vascular biomarkers other than the traditional risk factors with a special focus on the emerging -omics technologies...
October 14, 2015: European Heart Journal
Tim Schumann, Till Adhikary, Annika Wortmann, Florian Finkernagel, Sonja Lieber, Evelyn Schnitzer, Nathalie Legrand, Yvonne Schober, W Andreas Nockher, Philipp M Toth, Wibke E Diederich, Andrea Nist, Thorsten Stiewe, Uwe Wagner, Silke Reinartz, Sabine Müller-Brüsselbach, Rolf Müller
The nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) is a lipid ligand-inducible transcription factor associated with macrophage polarization. However, its function in tumor-associated macrophages (TAMs) has not been investigated to date. Here, we report the PPARβ/δ-regulated transcriptome and cistrome for TAMs from ovarian carcinoma patients. Comparison with monocyte-derived macrophages shows that the vast majority of direct PPARβ/δ target genes are upregulated in TAMs and largely refractory to synthetic agonists, but repressible by inverse agonists...
May 30, 2015: Oncotarget
He Tian, Weiyu Wang, Nan Zheng, Jianbo Cheng, Songli Li, Yangdong Zhang, Jiaqi Wang
UNLABELLED: Controlling heat stress (HS) is a global challenge for the dairy industry. However, simple and reliable biomarkers that aid the diagnoses of HS-induced metabolic disorders have not yet been identified. In this work, an integrated metabolomic and lipidomic approach using (1)H nuclear magnetic resonance and ultra-fast LC-MS was employed to investigate the discrimination of plasma metabolic profiles between HS-free and HS lactating dairy cows. Targeted detection using LC-MS in multiple reaction monitoring mode was used to verify the reliability of the metabolites as biomarker candidates...
July 1, 2015: Journal of Proteomics
Adam Beach, Vincent R Richard, Simon Bourque, Tatiana Boukh-Viner, Pavlo Kyryakov, Alejandra Gomez-Perez, Anthony Arlia-Ciommo, Rachel Feldman, Anna Leonov, Amanda Piano, Veronika Svistkova, Vladimir I Titorenko
We have previously revealed that exogenously added lithocholic bile acid (LCA) extends the chronological lifespan of the yeast Saccharomyces cerevisiae, accumulates in mitochondria and alters mitochondrial membrane lipidome. Here, we use quantitative mass spectrometry to show that LCA alters the age-related dynamics of changes in levels of many mitochondrial proteins, as well as numerous proteins in cellular locations outside of mitochondria. These proteins belong to 2 regulons, each modulated by a different mitochondrial dysfunction; we call them a partial mitochondrial dysfunction regulon and an oxidative stress regulon...
2015: Cell Cycle
Giulia Salvatore, Nathalie Bernoud-Hubac, Nathalie Bissay, Cyrille Debard, Patricia Daira, Emmanuelle Meugnier, Fabienne Proamer, Daniel Hanau, Hubert Vidal, Maurizio Aricò, Christine Delprat, Karène Mahtouk
Interleukin 17A (IL-17A) is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory diseases. In the field of immunometabolism, we have studied the impact of IL-17A on the lipid metabolism of human in vitro-generated monocyte-derived dendritic cells (DCs). Microarrays and lipidomic analysis revealed an intense remodeling of lipid metabolism induced by IL-17A in DCs. IL-17A increased 2-12 times the amounts of phospholipids, cholesterol, triglycerides, and cholesteryl esters in DCs. Palmitic (16:0), stearic (18:0), and oleic (18:ln-9c) acid were the main fatty acid chains present in DCs...
June 2015: Journal of Lipid Research
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