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Diacylglycerol lipase

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https://www.readbyqxmd.com/read/29785208/developing-cellulolytic-yarrowia-lipolytica-as-a-platform-for-the-production-of-valuable-products-in-consolidated-bioprocessing-of-cellulose
#1
Zhong-Peng Guo, Julien Robin, Sophie Duquesne, Michael Joseph O'Donohue, Alain Marty, Florence Bordes
Background: Both industrial biotechnology and the use of cellulosic biomass as feedstock for the manufacture of various commercial goods are prominent features of the bioeconomy. In previous work, with the aim of developing a consolidated bioprocess for cellulose bioconversion, we conferred cellulolytic activity of Yarrowia lipolytica , one of the most widely studied "nonconventional" oleaginous yeast species. However, further engineering this strain often leads to the loss of previously introduced heterologous genes due to the presence of multiple LoxP sites when using Cre -recombinase to remove previously employed selection markers...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29764424/long-non-coding-rna-neat1-modulated-abnormal-lipolysis-via-atgl-drives-hepatocellular-carcinoma-proliferation
#2
Xirui Liu, Yingjian Liang, Ruipeng Song, Guangchao Yang, Jihua Han, Yaliang Lan, Shangha Pan, Mingxi Zhu, Yao Liu, Yan Wang, Fanzheng Meng, Yifeng Cui, Jiabei Wang, Bo Zhang, Xuan Song, Zhaoyang Lu, Tongsen Zheng, Lianxin Liu
BACKGROUND: Abnormal metabolism, including abnormal lipid metabolism, is a hallmark of cancer cells. Some studies have demonstrated that the lipogenic pathway might promote the development of hepatocellular carcinoma (HCC). However, the role of the lipolytic pathway in HCC has not been elucidated. METHODS: We compared levels of adipose triglyceride lipase (ATGL) in human HCC and healthy liver tissues by real time PCR, western blot and immunohistochemistry. We measured diacylglycerol(DAG) and free fatty acid (FFA) levels in HCC cells driven by the NEAT1-ATGL axis and in HCC tissues...
May 15, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29614312/diacylglycerol-lipase-alpha-promotes-tumorigenesis-in-oral-cancer-by-cell-cycle-progression
#3
Yasuhiko Okubo, Atsushi Kasamatsu, Masanobu Yamatoji, Kazuaki Fushimi, Takashi Ishigami, Toshihiro Shimizu, Hiroki Kasama, Masashi Shiiba, Hideki Tanzawa, Katsuhiro Uzawa
Diacylglycerol lipase alpha (DAGLA), which catalyzes the hydrolysis of diacylglycerol to 2-arachidonoylglycerol and free fatty acid, is required for axonal growth during the brain development and for retrograde synaptic signaling at mature synapses. So far, no information was found regarding the possible role of DAGLA in human tumorigenesis. Thus, the current study sought to clarify the contribution of DAGLA in oral squamous cell carcinomas (OSCCs) and assess the clinical possibilities for OSCC treatment. Using real-time quantitative reverse transcription-polymerase chain reaction, immunoblotting, and immunohistochemistry, we found a significant up-regulation of DAGLA in OSCCs compared with normal cells and tissues both at mRNA and protein expression levels...
March 31, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29593725/perilipin-1-mediates-lipid-metabolism-homeostasis-and-inhibits-inflammatory-cytokine-synthesis-in-bovine-adipocytes
#4
Shiqi Zhang, Guowen Liu, Chuang Xu, Lei Liu, Qiang Zhang, Qiushi Xu, Hongdou Jia, Xiaobing Li, Xinwei Li
Dairy cows with ketosis displayed lipid metabolic disorder and high inflammatory levels. Adipose tissue is an active lipid metabolism and endocrine tissue and is closely related to lipid metabolism homeostasis and inflammation. Perilipin 1 (PLIN1), an adipocyte-specific lipid-coated protein, may be involved in the above physiological function. The aim of this study is to investigate the role of PLIN1 in lipid metabolism regulation and inflammatory factor synthesis in cow adipocytes. The results showed that PLIN1 overexpression upregulated the expression of fatty acid and triglyceride (TAG) synthesis molecule sterol regulator element-binding protein-1c (SREBP-1c) and its target genes, diacylglycerol acyltransferase (DGAT) 1, and DGAT2, but inhibited the expression of lipolysis enzymes hormone-sensitive lipase (HSL) and CGI-58 for adipose triglyceride lipase (ATGL), thus augmenting the fatty acids and TAG synthesis and inhibiting lipolysis...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29565900/mapping-in-vivo-target-interaction-profiles-of-covalent-inhibitors-using-chemical-proteomics-with-label-free-quantification
#5
Eva J van Rooden, Bogdan I Florea, Hui Deng, Marc P Baggelaar, Annelot C M van Esbroeck, Juan Zhou, Herman S Overkleeft, Mario van der Stelt
Activity-based protein profiling (ABPP) has emerged as a valuable chemical proteomics method to guide the therapeutic development of covalent drugs by assessing their on-target engagement and off-target activity. We recently used ABPP to determine the serine hydrolase interaction landscape of the experimental drug BIA 10-2474, thereby providing a potential explanation for the adverse side effects observed with this compound. ABPP allows mapping of protein interaction landscapes of inhibitors in cells, tissues and animal models...
April 2018: Nature Protocols
https://www.readbyqxmd.com/read/29551907/contribution-of-diacylglycerol-lipase-%C3%AE-to-pain-after-surgery
#6
Jennifer Luk, Yong Lu, Amanda Ackermann, Xiaoxue Peng, Diane Bogdan, Michelino Puopolo, David E Komatsu, Simon Tong, Iwao Ojima, Mario J Rebecchi, Martin Kaczocha
Background: Metabolism of the endocannabinoid 2-arachidonoylglycerol (2-AG) yields arachidonic acid (AA), the precursor to proalgesic eicosanoids including prostaglandin E2 (PGE2 ). Diacylglycerol lipase β (DAGLβ) is an enzyme that synthesizes 2-AG and its inhibition reduces eicosanoid levels and produces antinociceptive effects in models of inflammatory pain. Here we test whether inhibition of DAGLβ produces antinociceptive effects in a model of postoperative pain. Methods: Rats were administered the selective DAGLβ inhibitor KT109 or vehicle and underwent plantar incision...
2018: Journal of Pain Research
https://www.readbyqxmd.com/read/29509511/deletion-of-diacylglycerol-kinase-%C3%AE%C2%B5-confers-susceptibility-to-obesity-via-reduced-lipolytic-activity-in-murine-adipocytes
#7
Tomoyuki Nakano, Keiko Seino, Ichiro Wakabayashi, Diana M Stafforini, Matthew K Topham, Kaoru Goto
Lipid metabolism is closely involved with signal transduction and energy homeostasis. Excess calorie intake causes abnormal lipid metabolism, promoting obesity and insulin resistance. Diacylglycerol (DG) represents not only a lipidic second messenger but also an intermediate metabolite for triglyceride metabolism in the endoplasmic reticulum (ER). However, it remains undetermined how the roles of DG in signaling and energy homeostasis is regulated within the cell. Of DG kinases (DGKs), which are enzymes that phosphorylate DG, DGKε resides in the ER...
March 6, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29477030/association-between-alcoholism-and-the-gene-encoding-the-endocannabinoid-synthesizing-enzyme-diacylglycerol-lipase-alpha-in-the-japanese-population
#8
Hiroki Ishiguro, Susumu Higuchi, Tadao Arinami, Emmanuel S Onaivi
The endocannabinoid system has been recognized to be involved in neuropsychiatric diseases. 2-arachidonoyl glycerol (2-AG) is one of the two main endocannabinoids, and their regulation could play roles in disorders under environmental influence. This study investigated the involvement of the 2-AG biosynthesizing enzyme diacylglycerol lipase alpha (DAGLA) in the pathogenesis of alcoholism. We investigated a possible association between alcoholism and single nucleotide polymorphisms (SNPs) of the human DAGLA gene in the Japanese population...
May 2018: Alcohol
https://www.readbyqxmd.com/read/29476167/a-novel-variant-associated-with-hdl-c-levels-by-modifying-daglb-expression-levels-an-annotation-based-genome-wide-association-study
#9
Dan Zhou, Dandan Zhang, Xiaohui Sun, Zhiqiang Li, Yaqin Ni, Zhongyan Shan, Hong Li, Chengguo Liu, Shuai Zhang, Yi Liu, Ruizhi Zheng, Feixia Pan, Yimin Zhu, Yongyong Shi, Maode Lai
Although numbers of genome-wide association studies (GWAS) have been performed for serum lipid levels, limited heritability has been explained. Studies showed that combining data from GWAS and expression quantitative trait loci (eQTLs) signals can both enhance the discovery of trait-associated SNPs and gain a better understanding of the mechanism. We performed an annotation-based, multistage genome-wide screening for serum-lipid-level-associated loci in totally 6863 Han Chinese. A serum high-density lipoprotein cholesterol (HDL-C) associated variant rs1880118 (hg19 chr7:g...
February 23, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29468762/hypolipidemic-activity-and-mechanisms-of-the-total-phenylpropanoid-glycosides-from-ligustrum-robustum-roxb-blume-by-ampk-srebp-1c-pathway-in-hamsters-fed-a-high-fat-diet
#10
Runmei Yang, Xinxin Chu, Le Sun, Zhuoying Kang, Min Ji, Ying Yu, Ying Liu, Zhendan He, Nannan Gao
The aim of this study was to evaluate the hypolipidemic effect and mechanisms of total phenylpropanoid glycosides extracted from Ligustrum robustum (Roxb.) Blume (LRTPG) in hamsters fed a high-fat diet and to discover bioactive components in HepG2 cell model induced by oleic acid. LRTPG of high (1.2 g/kg), medium (0.6 g/kg), and low (0.3 g/kg) doses was administrated daily for 21 consecutive days in hamsters. We found that in hamsters fed a high-fat diet, LRTPG effectively reduced the concentrations of plasma triglycerides (TG), free fatty acid, total cholesterol, low-density lipoprotein cholesterol, and hepatic TG and total cholesterol...
April 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29467679/the-endocannabinoid-system-across-postnatal-development-in-transmembrane-domain-neuregulin-1-mutant-mice
#11
Rose Chesworth, Leonora E Long, Cynthia Shannon Weickert, Tim Karl
The use of cannabis is a well-established component risk factor for schizophrenia, particularly in adolescent individuals with genetic predisposition for the disorder. Alterations to the endocannabinoid system have been found in the prefrontal cortex of patients with schizophrenia. Thus, we assessed whether molecular alterations exist in the endocannabinoid signalling pathway during brain development in a mouse model for the schizophrenia risk gene neuregulin 1 ( Nrg1 ). We analysed transcripts encoding key molecules of the endocannabinoid system in heterozygous transmembrane domain Nrg1 mutant mice ( Nrg1 TM HET), which is known to have increased sensitivity to cannabis exposure...
2018: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/29458998/role-of-striatal-direct-pathway-2-arachidonoylglycerol-signaling-in-sociability-and-repetitive-behavior
#12
Brian C Shonesy, Walker P Parrish, Hala K Haddad, Jason R Stephenson, Rita Báldi, Rebecca J Bluett, Christian R Marks, Samuel W Centanni, Oakleigh M Folkes, Keeley Spiess, Shana M Augustin, Ken Mackie, David M Lovinger, Danny G Winder, Sachin Patel, Roger J Colbran
BACKGROUND: Endocannabinoid signaling plays an important role in regulating synaptic transmission in the striatum, a brain region implicated as a central node of dysfunction in autism spectrum disorder. Deficits in signaling mediated by the endocannabinoid 2-arachidonoylglycerol (2-AG) have been reported in mouse models of autism spectrum disorder, but a causal role for striatal 2-AG deficiency in phenotypes relevant to autism spectrum disorder has not been explored. METHODS: Using conditional knockout mice, we examined the electrophysiological, biochemical, and behavioral effects of 2-AG deficiency by deleting its primary synthetic enzyme, diacylglycerol lipase α (DGLα), from dopamine D1 receptor-expressing or adenosine A2a receptor-expressing medium spiny neurons (MSNs) to determine the role of 2-AG signaling in striatal direct or indirect pathways, respectively...
December 28, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/29401481/kinetic-study-of-lipase-catalyzed-glycerolysis-of-palm-olein-using-lipozyme-tlim-in-solvent-free-system
#13
Thomas Shean Yaw Choong, Chiou Moi Yeoh, Eng-Tong Phuah, Wai-Lin Siew, Yee-Ying Lee, Teck-Kim Tang, Luqman Chuah Abdullah
Diacylglycerol (DAG) and monoacylglycerol (MAG) are two natural occurring minor components found in most edible fats and oils. These compounds have gained increasing market demand owing to their unique physicochemical properties. Enzymatic glycerolysis in solvent-free system might be a promising approach in producing DAG and MAG-enriched oil. Understanding on glycerolysis mechanism is therefore of great importance for process simulation and optimization. In this study, a commercial immobilized lipase (Lipozyme TL IM) was used to catalyze the glycerolysis reaction...
2018: PloS One
https://www.readbyqxmd.com/read/29375048/effect-of-tachycardia-on-mrna-andf-protein-expression-of-the-principal-components-of-the-lipolytic-system-in-the-rat-s-heart-ventricles
#14
B Wojcik, A Miklosz, P Zabielski, A Chabowski, J Gorski
There is a convincing piece of evidence showing that most of free fatty acids (FFA) entering cardiomyocytes are first esterified into triacylglycerols (TG) before being oxidized or used for synthesis of complex lipids. The enzyme adipose triglyceride lipase (ATGL) initiates lipolysis of TG by hydrolyzing the first ester bond of the compound. As a result, free fatty acid and diacylglycerol (DG) are released in that process. Finally, DG may be further hydrolyzed by the enzyme hormone sensitive lipase (HSL). The aim of the present study was to examine effect of tachycardia on mRNA and protein expression of ATGL, CGI-58 (an activator of ATGL), G0S2 (an inhibitor of ATGL) and HSL in the left and right ventricle of the rat...
October 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29297136/production-of-fame-and-faee-via-alcoholysis-of-sunflower-oil-by-eversa-lipases-immobilized-on-hydrophobic-supports
#15
Daniela Remonatto, J Vladimir de Oliveira, J Manuel Guisan, Débora de Oliveira, Jorge Ninow, Gloria Fernandez-Lorente
The performance of two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 immobilized in different supports was investigated. The two lipases were adsorbed on four different hydrophobic supports. Interesting results were obtained for both lipases and for the four supports. However, the most active derivative was prepared by immobilization of Eversa® Transform 2.0 on Sepabeads C-18. Ninety-nine percent of fatty acid ethyl ester was obtained, in 3 h at 40 °C, by using hexane as solvent, a molar ratio of 4:1 (ethanol/oil), and 10 wt% of immobilized biocatalyst...
January 3, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29287386/selective-synthesis-of-partial-glycerides-of-conjugated-linoleic-acids-via-modulation-of-the-catalytic-properties-of-lipases-by-immobilization-on-different-supports
#16
Carlos M Verdasco-Martín, Eduardo Garcia-Verdugo, Raul Porcar, Roberto Fernandez-Lafuente, Cristina Otero
Lipases B from Candida antarctica (CALB), Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL) were immobilized on octadecyl methacylate (OM) or octadecyl methacrylate (OMC) beads. Their specific activity and regioselectivity were studied in the synthesis of conjugated linoleic acid (CLA) partial glycerides, which presented nutraceutical properties. TLL derivatives were poor catalysts. Novozym® 435 was much better than Lipozyme® RM IM. RML activity (a GRAS enzyme) was modulated via immobilization. After only 3 h, OM-RML gave the highest CLA conversion (54% at 40 °C with 1:3 M ratio of glycerol to CLA)...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29193524/effect-of-site-specific-peptide-tag-labeling-on-the-biocatalytic-properties-of-thermoalkalophilic-lipase-from-geobacillus-thermocatenulatus
#17
Oscar Romero, Blanca de Las Rivas, David Lopez-Tejedor, Jose M Palomo
Tailor-made peptides were investigated for site-specific tag labeling of Geobacillus thermocatenulatus lipase (GTL). GTL was first genetically modified by introducing a unique cysteine on the lid site of the enzyme to produce two variants (GTLσ-A193C and GTLσ-S196C). Chemical modification was performed by using a small library of cysteine-containing peptides. The synthesized peptide-lipase biocatalysts were highly stable, more active, more specific, and more selective toward different substrates than unmodified GTL...
February 16, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29184702/pharmacological-inhibition-of-diacylglycerol-acyltransferase-1-and-insights-into-postprandial-gut-peptide-secretion
#18
Benjamin S Maciejewski, Tara B Manion, Claire M Steppan
AIM: To examine the role that enzyme Acyl-CoA:diacylglycerol acyltransferase-1 (DGAT1) plays in postprandial gut peptide secretion and signaling. METHODS: The standard experimental paradigm utilized to evaluate the incretin response was a lipid challenge. Following a lipid challenge, plasma was collected via cardiac puncture at each time point from a cohort of 5-8 mice per group from baseline at time zero to 10 h. Incretin hormones [glucagon like peptide-1 (GLP-1), peptide tyrosine-tyrosine (PYY) and glucose dependent insulinotropic polypeptide (GIP)] were then quantitated...
November 15, 2017: World Journal of Gastrointestinal Pathophysiology
https://www.readbyqxmd.com/read/29178188/characterization-of-the-enzymatic-activity-and-physiological-function-of-the-lipid-droplet-associated-triacylglycerol-lipase-atobl1
#19
Anna Ophelia Müller, Till Ischebeck
Similar to seeds, pollen tubes contain lipid droplets that store triacylglycerol (TAG), but the fate of this TAG as well as the enzymes involved in its breakdown are unknown. Therefore, two potential TAG lipases from tobacco and Arabidopsis, NtOBL1 (Oil body lipase 1) and AtOBL1, were investigated, especially with respect to their importance for pollen tube growth. We expressed NtOBL1 and AtOBL1 as fluorescent fusion proteins to study their localization by confocal microscopy. Furthermore, we overexpressed AtOBL1 in Nicotiana benthamiana leaves to characterize it enzymatically...
February 2018: New Phytologist
https://www.readbyqxmd.com/read/29174396/plc%C3%AE-1-potential-arbitrator-of-cancer-progression
#20
REVIEW
Hyun-Jun Jang, Pann-Ghill Suh, Yu Jin Lee, Kyeong Jin Shin, Lucio Cocco, Young Chan Chae
Phospholipase C (PLC) is an essential mediator of cellular signaling. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP3 ) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca2+ ) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. PLCγ1, one of the primary subtypes of PLC, is directly activated by membrane receptors, including receptor tyrosine kinases (RTKs), and adhesion receptors such as integrin...
January 2018: Advances in Biological Regulation
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