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Dental pulp stem cells

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https://www.readbyqxmd.com/read/28210430/growth-kinetics-and-characterization-of-human-dental-pulp-stem-cells-comparison-between-third-molar-and-first-premolar-teeth
#1
Davood Mehrabani, Parisa Mahdiyar, Kianoosh Torabi, Reza Robati, Shahrokh Zare, Mehdi Dianatpour, Amin Tamadon
BACKGROUND: Dental pulp stem cells (DPSCs) play an important role in tissue regeneration. This study compares the growth kinetics and characterization of third molar and first premolar human DPSCs. MATERIAL AND METHODS: Dental pulp tissues were isolated from human first premolar and third molar teeth and were digested by treating them with collagenase type I. Single-cell suspensions from each dental pulp were seeded in T25 culture flasks and the media were replaced every 3 days until 70% confluence...
February 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28209631/hdpscs-fabricate-a-vascularised-woven-bone-tissue-a-new-tool-in-bone-tissue-engineering
#2
Francesca Paino, Marcella La Noce, Alessandra Giuliani, Alfredo De Rosa, Serena Mazzoni, Luigi Laino, Evzen Amler, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells successfully used in human bone tissue engineering. To better establish if these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim to obtain a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal-vascular dental pulp fraction, using appropriate markers and cultured. Growth curves, expression of bone-related markers, calcification, angiogenesis as well as in vivo transplantation assay were performed...
February 16, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28205268/the-effect-of-delta-like-1-homologue-on-the-proliferation-and-odontoblastic-differentiation-in-human-dental-pulp-stem-cells
#3
Shengcai Qi, Yanhong Yan, Yue Wen, Jialiang Li, Jing Wang, Fubo Chen, Xiaoshan Tang, Guangwei Shang, Yuanzhi Xu, Raorao Wang
INTRODUCTION: This study aimed to investigate the functions of delta-like homologue 1 (DLK1) in the proliferation and differentiation of human dental pulp stem cells (hDPSCs). METHODS: Immunohistochemical analysis was used to determine the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), DLK1, NOTCH1 and p-ERK1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs. The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay respectively...
February 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28198096/treated-dentin-matrix-paste-as-a-novel-pulp-capping-agent-for-dentin-regeneration
#4
Jinlong Chen, Caiyun Cui, Xiangchen Qiao, Bo Yang, Mei Yu, Weihua Guo, Weidong Tian
Regenerating dentin and preserving pulp vitality are the two key targets for the treatment of dental pulp exposure. Calcium hydroxide (CH), the widely used capping agent, may induce potential tunnel defect in reparative dentin and cause inflammation or even necrosis in pulp tissues. This study aimed to produce a novel pulp capping agent with better bioactivities. Treated dentin matrix (TDM) paste (TDMP) was fabricated consisting of TDM powder and aqueous TDM extract. The chemical and biological characteristics of TDMP were investigated, and its effect on the odontogenic differentiation of dental pulp stem cells explored at gene and protein level; the therapeutic effect for pulp exposure in miniature swine was further verified...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28196330/decellularized-human-dental-pulp-as-a-scaffold-for-regenerative-endodontics
#5
J S Song, K Takimoto, M Jeon, J Vadakekalam, N B Ruparel, A Diogenes
Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alternative to conventional root canal treatment for immature teeth. These procedures rely on the transfer of apically positioned stem cells, including stem cells of the apical papilla (SCAP), into the root canal system...
February 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28179933/role-of-cd146-enrichment-in-purification-of-stem-cells-derived-from-dental-pulp-polyp
#6
Maryam Sadat Tavangar, Seyed-Mojtaba Hosseini, Ali Dehghani-Nazhvani, Ahmad Monabati
INTRODUCTION: Hyperplastic pulpitis (pulp polyp) tissues contains cells with stem cell properties similar to that of the dental pulp stem cells (DPSCs). It has also been shown that CD146 enrichment can homogenize the cultures of DPSCs and enhance the colony forming potentials of their cultures. This study determines whether CD146 enrichment can help purifying the stem cells from heterogeneous cultures of the pulp polyp derived stem cells (PPSCs). METHODS AND MATERIALS: Healthy dental pulps and pulp polyp tissues were enzymatically digested and the harvested single cells were sorted according to the presence of CD146 marker...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28159719/improvement-of-osteogenesis-in-dental-pulp-pluripotent-like-stem-cells-by-oligopeptide-modified-poly-%C3%A3-amino-ester-s
#7
Raquel Núñez-Toldrà, Pere Dosta, Sheyla Montori, Víctor Ramos, Maher Atari, Salvador Borrós
: Controlling pluripotent stem cell differentiation via genetic manipulation is a promising technique in regenerative medicine. However, the lack of safe and efficient delivery vehicles limits this application. Recently, a new family of poly (β-amino ester)s (pBAEs) with oligopeptide-modified termini showing high transfection efficiency of both siRNA and DNA plasmid has been developed. In this study, oligopeptide-modified pBAEs were used to simultaneously deliver anti-OCT3/4 siRNA, anti-NANOG siRNA, and RUNX2 plasmid to cells from the dental pulp with pluripotent-like characteristics (DPPSC) in order to promote their osteogenic differentiation...
January 31, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28157689/differential-mineralization-of-human-dental-pulp-stem-cells-on-diverse-polymers
#8
Christian Apel, Patricia Buttler, Jochen Salber, Anandhan Dhanasingh, Sabine Neuss
In tissue engineering, biomaterials are used as scaffolds for spatial distribution of specific cell types. Biomaterials can potentially influence cell proliferation and extracellular matrix formation, both in positive and negative ways. The aim of the present study was to investigate and compare mineralized matrix production of human dental pulp stem cells (DPSC), cultured on 17 different well-characterized polymers. Osteogenic differentiation of DPSC was induced for 21 days on biomaterials using dexamethasone, L-ascorbic-acid-2-phosphate, and sodium β-glycerophosphate...
February 3, 2017: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/28148303/variation-in-human-dental-pulp-stem-cell-ageing-profiles-reflect-contrasting-proliferative-and-regenerative-capabilities
#9
Amr Alraies, Nadia Y A Alaidaroos, Rachel J Waddington, Ryan Moseley, Alastair J Sloan
BACKGROUND: Dental pulp stem cells (DPSCs) are increasingly being recognized as a viable cell source for regenerative medicine. Although significant variations in their ex vivo expansion are well-established, DPSC proliferative heterogeneity remains poorly understood, despite such characteristics influencing their regenerative and therapeutic potential. This study assessed clonal human DPSC regenerative potential and the impact of cellular senescence on these responses, to better understand DPSC functional behaviour...
February 2, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28148268/mesenchymal-stem-cells-key-players-in-cancer-progression
#10
REVIEW
Sarah M Ridge, Francis J Sullivan, Sharon A Glynn
Tumour progression is dependent on the interaction between tumour cells and cells of the surrounding microenvironment. The tumour is a dynamic milieu consisting of various cell types such as endothelial cells, fibroblasts, cells of the immune system and mesenchymal stem cells (MSCs). MSCs are multipotent stromal cells that are known to reside in various areas such as the bone marrow, fat and dental pulp. MSCs have been found to migrate towards inflammatory sites and studies have shown that they also migrate towards and incorporate into the tumour...
February 1, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28144997/explant-culture-an-advantageous-method-for-isolation-of-mesenchymal-stem-cells-from-human-tissues
#11
REVIEW
Fatemeh Hendijani
Mesenchymal stem cell (MSC) research progressively moves towards clinical phases. Accordingly, a wide range of different procedures were presented in the literature for MSC isolation from human tissues; however, there is not yet any close focus on the details to offer precise information for best method selection. Choosing a proper isolation method is a critical step in obtaining cells with optimal quality and yield in companion with clinical and economical considerations. In this concern, current review widely discusses advantages of omitting proteolysis step in isolation process and presence of tissue pieces in primary culture of MSCs, including removal of lytic stress on cells, reduction of in vivo to in vitro transition stress for migrated/isolated cells, reduction of price, processing time and labour, removal of viral contamination risk, and addition of supporting functions of extracellular matrix and released growth factors from tissue explant...
February 1, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28141971/paracrine-maturation-and-migration-of-sh-sy5y-cells-by-dental-pulp-stem-cells
#12
P Gervois, E Wolfs, Y Dillen, P Hilkens, J Ratajczak, R B Driesen, T Vangansewinkel, A Bronckaers, B Brône, T Struys, I Lambrichts
Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired by the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute the lost tissue or that stimulate host repair. The present study aimed to evaluate the paracrine effects of human dental pulp stem cells (hDPSCs) on the migration and neural maturation of human SH-SY5Y neuroblastoma cells. The hDPSC secretome had a significant chemoattractive effect on SH-SY5Y cells as shown by a transwell assay...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28117963/chitosan-intercalated-montmorillonite-poly-vinyl-alcohol-nanofibers-as-a-platform-to-guide-neuron-like-differentiation-of-human-dental-pulp-stem-cells
#13
Hatef Ghasemi Hamidabadi, Zahra Rezvani, Maryam Nazm Bojnordi, Haji Shirinzadeh, Alexander M Seifalian, Mohammad Taghi Joghataei, Mojgan Razaghpour, Abbas Alibakhshi, Abolfazl Yazdanpanah, Maryam Salimi, Masoud Mozafari, Aleksandra M Urbanska, Rui L Reis, Subhas C Kundu, Mazaher Gholipourmalekabadi
In this study, we present a novel chitosan-intercalated montmorillonite/Poly(Vinyl alcohol) (OMMT/PVA) nanofibrous mesh as a microenvironment for guiding differentiation of human dental pulp stem cells (hDPSCs) toward neuron-like cells. The OMMT was prepared through ion exchange reaction between the montmorillonite (MMT) and chitosan. The PVA solutions containing various concentrations of OMMT were electrospun to form 3-D OMMT-PVA nanofibrous meshes. The biomechanical and biological characteristics of the nanofibrous meshes were evaluated by ATR-FTIR, XRD, SEM, MTT, LDH specific activity, contact angle, and DAPI staining...
January 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28117904/the-effect-of-accelerated-mineral-trioxide-aggregate-on-odontoblastic-differentiation-in-dental-pulp-stem-cell-niches
#14
P Kulan, O Karabiyik, G T Kose, B Kargul
AIM: To investigate the effect of accelerated-set mineral trioxide aggregate (MTA) on the proliferation and odontoblastic differentiation of human dental pulp cell niches (DPSC). METHODOLOGY: ProRoot White MTA (WMTA) (Dentsply Tulsa Dental, Johnson City, TN, USA) was mixed with various additives, which included distilled water, 2.5% disodium hydrogen phosphate (Na2 HPO4 )(Merck, Darmstadt, Germany) and 5% calcium chloride (CaCl2 )(Merck). DPSC niches extracted from third molars were cultured directly on MTA in the culture medium...
January 24, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28109162/odontoblastic-differentiation-of-dental-pulp-stem-cells-from-healthy-and-carious-teeth-on-an-original-pcl-based-3d-scaffold
#15
A Louvrier, E Euvrard, L Nicod, G Rolin, F Gindraux, L Pazart, C Houdayer, P Y Risold, F Meyer, C Meyer
AIMS: To isolate and characterize Dental Pulp Stem Cells (DPSCs) obtained from carious and healthy mature teeth extracted when conservative treatment was not possible or for orthodontic reasons; to evaluate the ability of DPSCs to colonize, proliferate, and differentiate into functional odontoblast-like cells when cultured onto a polycaprolactone cone made by jet-spraying and prototyped into a design similar to a gutta-percha cone. METHODOLOGY: DPSCs were obtained from 9 carious and 12 healthy mature teeth...
January 21, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28106508/gelma-encapsulated-hdpscs-and-huvecs-for-dental-pulp-regeneration
#16
A Khayat, N Monteiro, E E Smith, S Pagni, W Zhang, A Khademhosseini, P C Yelick
Pulpal revascularization is commonly used in the dental clinic to obtain apical closure of immature permanent teeth with thin dentinal walls. Although sometimes successful, stimulating bleeding from the periapical area of the tooth can be challenging and in turn may deleteriously affect tooth root maturation. Our objective here was to define reliable methods to regenerate pulp-like tissues in tooth root segments (RSs). G1 RSs were injected with human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) encapsulated in 5% gelatin methacrylate (GelMA) hydrogel...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28106505/pulp-vascularization-during-tooth-development-regeneration-and-therapy
#17
C Rombouts, T Giraud, C Jeanneau, I About
The pulp is a highly vascularized tissue situated in an inextensible environment surrounded by rigid dentin walls, with the apical foramina being the only access. The pulp vascular system is not only responsible for nutrient supply and waste removal but also contributes actively to the pulp inflammatory response and subsequent regeneration. This review discusses the underlying mechanisms of pulp vascularization during tooth development, regeneration, and therapeutic procedures, such as tissue engineering and tooth transplantation...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28105979/human-dental-pulp-stem-cells-are-more-effective-than-human-bone-marrow-derived-mesenchymal-stem-cells-in-cerebral-ischemic-injury
#18
Miyeoun Song, Jae-Hyung Lee, Jinhyun Bae, Youngmin Bu, Eun-Cheol Kim
We compared the therapeutic effects and mechanism of transplanted human dental pulp stem cells (hDPSCs) and human bonemarrow-derived mesenchymal stem cells (hMSCs) in a stroke model of rats and an <i>in vitro</i> ischemic model. Both hDPSCs- and hMSCs- intravenously injected rats 24&#8201;h after middle cerebral artery occlusion showed improved functional recovery and reduced infarct volume versus control rats, but the hDPSCs group showed greater reduction in infarct volume than hMSCs. The positive area for the endothelial cell marker was greater in the lesion boundary areas in the hDPSCs group than with hMSCs...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28098169/ifn-%C3%AE-regulates-human-dental-pulp-stem-cells-behavior-via-nf-%C3%AE%C2%BAb-and-mapk-signaling
#19
Xinyao He, Wenkai Jiang, Zhirong Luo, Tiejun Qu, Zhihua Wang, Ningning Liu, Yaqing Zhang, Paul R Cooper, Wenxi He
During caries, dental pulp expresses a range of pro-inflammatory cytokines in response to the infectious challenge. Interferon gamma (IFN-γ) is a dimerized soluble cytokine, which is critical for immune responses. Previous study has demonstrated that IFN-γ at relative high concentration (100 ng/mL) treatment improved the impaired dentinogenic and immunosuppressive regulatory functions of disease-derived dental pulp stem cells (DPSCs). However, little is known about the regulatory effects of IFN-γ at relative low concentration on healthy DPSC behavior (including proliferation, migration, and multiple-potential differentiation)...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28079995/effects-of-cell-cycle-phases-on-the-induction-of-dental-pulp-stem-cells-toward-dopaminergic-like-cells
#20
Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Premasangery Kathirvaloo, Sabri Musa, Noor Hayaty Abu Kasim
Parkinson's disease (PD) is characterized by tremors and cognitive issues, and is due to the death of dopaminergic (DA-ergic) neurons in brain circuits that are responsible for producing neurotransmitter dopamine (DA). Currently, cell replacement therapies are underway to overcome existing therapeutic approaches such as drug treatments and electrical stimulation. Among the widely available sources, dental pulp stem cells from deciduous teeth (DPSC) have gained popularity due to their neural crest origin and inherent propensity toward neuronal lineage...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
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