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sharpey's fibers

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https://www.readbyqxmd.com/read/27843719/cranial-bone-histology-of-metoposaurus-krasiejowensis-amphibia-temnospondyli-from-the-late-triassic-of-poland
#1
Kamil Gruntmejer, Dorota Konietzko-Meier, Adam Bodzioch
In this study, 21 skull bones of Metoposaurus krasiejowensis from the Late Triassic of Poland were investigated histologically. Dermal bones show a diploë structure, with an ornamented external surface. The ridges consist of mostly well vascularized parallel-fibered bone; the valleys are built of an avascular layer of lamellar bone. The thick middle region consists of cancellous bone, with varying porosity. The thin and less vascularized internal cortex consists of parallel-fibered bone. The numerous Sharpey's fibers and ISF are present in all bones...
2016: PeerJ
https://www.readbyqxmd.com/read/27833794/three-dimensional-paleohistology-of-the-scale-and-median-fin-spine-of-lophosteus-superbus-pander-1856
#2
Anna Jerve, Qingming Qu, Sophie Sanchez, Henning Blom, Per Erik Ahlberg
Lophosteus superbus is one of only a handful of probable stem-group osteichthyans known from the fossil record. First collected and described in the late 19th century from the upper Silurian Saaremaa Cliff locality in Estonia, it is known from a wealth of disarticulated scales, fin spines, and bone fragments. In this study we provide the first description of the morphology and paleohistology of a fin spine and scale from Lophosteus using virtual thin sections and 3D reconstructions that were segmented using phase-contrast synchrotron X-ray microtomography...
2016: PeerJ
https://www.readbyqxmd.com/read/27583044/collagen-hydrogel-scaffold-and-fibroblast-growth-factor-2-accelerate-periodontal-healing-of-class-ii-furcation-defects-in-dog
#3
Takehito Momose, Hirofumi Miyaji, Akihito Kato, Kosuke Ogawa, Takashi Yoshida, Erika Nishida, Syusuke Murakami, Yuta Kosen, Tsutomu Sugaya, Masamitsu Kawanami
OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system...
2016: Open Dentistry Journal
https://www.readbyqxmd.com/read/27471309/live-tissue-imaging-to-elucidate-mechanical-modulation-of-stem-cell-niche-quiescence
#4
Nicole Y C Yu, Connor A O'Brien, Iveta Slapetova, Renee M Whan, Melissa L Knothe Tate
: : The periosteum, a composite cellular connective tissue, bounds all nonarticular bone surfaces. Like Velcro, collagenous Sharpey's fibers anchor the periosteum in a prestressed state to the underlying bone. The periosteum provides a niche for mesenchymal stem cells. Periosteal lifting, as well as injury, causes cells residing in the periosteum (PDCs) to change from an immobile, quiescent state to a mobile, active state. The physical cues that activate PDCs to home to and heal injured areas remain a conundrum...
July 28, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27285944/parathyroid-hormone-intermittent-administration-promotes-delay-on-rat-incisor-eruption
#5
M A D Silva, D F P Vasconcelos, M R Marques, S P Barros
OBJECTIVE: This study evaluated the influence of parathyroid hormone (PTH) (1-34) intermittent administration on rat eruption rates of lower incisors under normo, hyper and hypofunctional conditions, Sharpey fibers insertion, and alveolar bone formation. MATERIALS AND METHODS: Wistar male rats received PTH (1-34) three times a week during the entire experimental period, 31days. Control animals received the same concentration of the vehicle solution during the same period...
September 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27194857/mounting-ground-sections-of-teeth-cyanoacrylate-adhesive-versus-canada-balsam
#6
Manogna Rl Vangala, Amrutha Rudraraju, R V Subramanyam
INTRODUCTION: Hard tissues can be studied by either decalcification or by preparing ground sections. Various mounting media have been tried and used for ground sections of teeth. However, there are very few studies on the use of cyanoacrylate adhesive as a mounting medium. AIMS: The aim of our study was to evaluate the efficacy of cyanoacrylate adhesive (Fevikwik™) as a mounting medium for ground sections of teeth and to compare these ground sections with those mounted with Canada balsam...
January 2016: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/27044216/-effect-of-hamstring-tendon-transfected-with-adenovirus-mediated-transforming-growth-factor-%C3%AE-%C3%A2-gene-on-histomorphology-of-tendon-bone-interface-healing-after-anterior-cruciate-ligament-reconstruction-in-rabbits
#7
Wang Xiaoxu, Rongfan Zhai, Yang Juntao, Shouhong Zhou, Wenfu Tan, Guanghua Tan
OBJECTIVE: To investigate the effect of hamstring tendon transfected with adenovirus-mediated transforming growth factor β₁ (AdTGF-β₁) genes on the histomorphology of tendon-bone interface healing after anterior cruciate ligament (ACL) reconstruction in rabbits. METHODS: AdTGF-β₁ and AdGFP were diluted to 5 x 10⁸ PFU/mL with DMEM. Forty-eight New Zealand white rabbits were divided into 3 groups randomly (n=16), weighing 1.6-2.5 kg for ACL reconstruction with hamstring tendon autograft...
December 2015: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/26990720/enhancement-of-polyethylene-terephthalate-artificial-ligament-graft-osseointegration-using-a-periosteum-patch-in-a-goat-model
#8
Z Dai, W Bao, S Li, H Li, J Jiang, S Chen
The purpose of this study is to investigate whether a periosteum patch could enhance polyethylene terephthalate (PET) artificial ligament graft osseointegration in a bone tunnel. 12 female goats underwent ACL reconstruction with a PET artificial ligament graft in the right knees. Right knees in 6 goats were reconstructed with periosteum patch-enveloped PET grafts (Periosteum group) in the tibia bone tunnel, whereas the other 6 goats had no periosteum patch and served as the Control group. All the goats were sacrificed at 12 months after surgery...
June 2016: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/26875269/-experimental-study-on-different-concentration-ratios-of-osteoprotegerin-combined-with-deproteinized-bone-on-bone-tunnel-after-anterior-cruciate-ligament-reconstruction
#9
Bing Wei, Guoyao Zou, Enhong Song, Lincong Guan
OBJECTIVE: To investigate the effects of different concentrations of osteoprotegerin (OPG) combined with deproteinized bone (DPB) on the bone tunnel after the anterior cruciate ligament (ACL) reconstruction. METHODS: The femoral epiphyseal side was harvested from newborn calf, and allogenic DPB were prepared by hydrogen peroxide-chloroform/methanol method. Then, DPB were immersed in 3 concentrations levels of OPG (30, 60, 100 µg/mL) and 3 concentration ratios (30%, 60%, 100%) of the gel complex were prepared...
November 2015: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/26790583/adipose-derived-regenerative-cells-promote-tendon-bone-healing-in-a-rabbit-model
#10
Masahiro Kosaka, Junsuke Nakase, Katsuhiro Hayashi, Hiroyuki Tsuchiya
PURPOSE: To evaluate the therapeutic effect of adipose-derived regenerative cell (ADRC) administration on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model. METHODS: ACL reconstruction with semitendinosus tendon autograft was performed in the right knees of adult white rabbits. Eighty rabbits were divided into 2 groups: the treatment group, in which the graft was coated with ADRCs mixed in a fibrin glue carrier during surgery, and the control group, in which the graft was coated with fibrin glue only...
May 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/26636129/strain-guided-mineralization-in-the-bone-pdl-cementum-complex-of-a-rat-periodontium
#11
Kathryn Grandfield, Ralf Peter Herber, Ling Chen, Sabra Djomehri, Caleb Tam, Ji-Hyun Lee, Evan Brown, Wood R Woolwine, Don Curtis, Mark Ryder, Jim Schuck, Samuel Webb, William Landis, Sunita Ho
OBJECTIVE: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)-bone and PDL-cementum attachment sites and within the tissues per se. DESIGN: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06N for 14 days on molars in a rat model. The associated changes in functional space between tooth and bone, mineral formation and resorbing events at the PDL-bone and PDL-cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, AFM-based nanoindentation technique, and were correlated with histochemical stains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate-resistant acid phosphatase (TRAP) staining...
December 1, 2015: Bone Reports
https://www.readbyqxmd.com/read/26427056/structural-and-ultrastructural-analysis-of-the-cervical-discs-of-young-and-elderly-humans
#12
COMPARATIVE STUDY
Ricardo Braganca de Vasconcellos Fontes, Josemberg Silva Baptista, Said Rahnamaye Rabbani, Vincent C Traynelis, Edson Aparecido Liberti
Several studies describing the ultrastructure and extracellular matrix (ECM) of intervertebral discs (IVDs) involve animal models and specimens obtained from symptomatic individuals during surgery for degenerative disease or scoliosis, which may not necessarily correlate to changes secondary to normal aging in humans. These changes may also be segment-specific based on different load patterns throughout life. Our objective was to describe the ECM and collagen profile of cervical IVDs in young (G1 - <35 years) and elderly (G2 - >65 years) presumably-asymptomatic individuals...
2015: PloS One
https://www.readbyqxmd.com/read/26026303/the-armored-carapace-of-the-boxfish
#13
Wen Yang, Steven E Naleway, Michael M Porter, Marc A Meyers, Joanna McKittrick
The boxfish (Lactoria cornuta) has a carapace consisting of dermal scutes with a highly mineralized surface plate and a compliant collagen base. This carapace must provide effective protection against predators as it comes at the high cost of reduced mobility and speed. The mineralized hydroxyapatite plates, predominantly hexagonal in shape, are reinforced with raised struts that extend from the center toward the edges of each scute. Below the mineralized plates are non-mineralized collagen fibers arranged in through-the-thickness layers of ladder-like formations...
September 2015: Acta Biomaterialia
https://www.readbyqxmd.com/read/25963390/mineralization-defects-in-cementum-and-craniofacial-bone-from-loss-of-bone-sialoprotein
#14
B L Foster, M Ao, C Willoughby, Y Soenjaya, E Holm, L Lukashova, A B Tran, H F Wimer, P M Zerfas, F H Nociti, K R Kantovitz, B D Quan, E D Sone, H A Goldberg, M J Somerman
Bone sialoprotein (BSP) is a multifunctional extracellular matrix protein found in mineralized tissues, including bone, cartilage, tooth root cementum (both acellular and cellular types), and dentin. In order to define the role BSP plays in the process of biomineralization of these tissues, we analyzed cementogenesis, dentinogenesis, and osteogenesis (intramembranous and endochondral) in craniofacial bone in Bsp null mice and wild-type (WT) controls over a developmental period (1-60 days post natal; dpn) by histology, immunohistochemistry, undecalcified histochemistry, microcomputed tomography (microCT), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and quantitative PCR (qPCR)...
September 2015: Bone
https://www.readbyqxmd.com/read/25882182/efficacy-and-safety-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-anterior-cruciate-ligament-reconstruction-of-a-rabbit-model-new-strategy-to-enhance-tendon-graft-healing
#15
Ki-Mo Jang, Hong Chul Lim, Woon Yong Jung, Sang Won Moon, Joon Ho Wang
PURPOSE: To investigate whether non-autologous transplantation of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) could be integrated safely at the bone-tendon junction without immune rejection and could enhance bone-tendon healing effectively during anterior cruciate ligament (ACL) reconstruction in an animal model. METHODS: ACL reconstructions using hamstring tendons were performed in 30 adult rabbits. The bone tunnels were treated with hUCB-MSCs or were untreated...
August 2015: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/25825137/the-role-of-an-octacalcium-phosphate-in-the-re-formation-of-infraspinatus-tendon-insertion
#16
Yoshiaki Itoigawa, Osamu Suzuki, Hirotaka Sano, Takahisa Anada, Takuto Handa, Taku Hatta, Yoshiyuki Kuwahara, Atsushi Takahashi, Yushi Ezoe, Kazuo Kaneko, Eiji Itoi
BACKGROUND: To improve the success rate of rotator cuff repair, we investigated whether octacalcium phosphate (OCP) with gelatin (Gel) vehicle had a positive effect on tendon-to-bone healing. METHODS: We assessed the histologic characteristics of the tendon-to-bone healing using the rabbit rotator cuff repair model. We divided the shoulders into 3 groups: control (without OCP/Gel composite), OCP/Gel composite (OCP+group), and Gel alone without OCP (Gel group) to evaluate the effectiveness of gelatin...
July 2015: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/25239932/local-application-of-strontium-in-a-calcium-phosphate-cement-system-accelerates-healing-of-soft-tissue-tendon-grafts-in-anterior-cruciate-ligament-reconstruction-experiment-using-a-rabbit-model
#17
Guan-Ming Kuang, W P Yau, William W Lu, K Y Chiu
BACKGROUND: Healing of soft tissue tendon grafts within the bone tunnel in anterior cruciate ligament (ACL) reconstruction is known to be slower than that of bone-patellar tendon-bone grafts. There are attempts to accelerate healing of the graft within the bone tunnel. One of the methods is the use of strontium-enriched calcium phosphate cement (Sr-CPC). Early results in animal studies have been encouraging, although it is not known whether the accelerated healing was solely caused by the effect of strontium within the cement or by the calcium phosphate cement (CPC) itself...
December 2014: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/24746398/schneiderian-membrane-detachment-using-transcrestal-hydrodynamic-ultrasonic-cavitational-sinus-lift-a-human-cadaver-head-study-and-histologic-analysis
#18
Angelo Troedhan, Andreas Kurrek, Marcel Wainwright, Siegfried Jank
PURPOSE: Recent studies have suggested the osteogenic layer of the periosteum at the base of the sinus membrane to play a key role in bone regeneration after sinus lift procedures. Thus, atraumatic detachment of the sinus membrane with an intact periosteum seems mandatory. The present histologic study of fresh human cadaver heads investigated the detachment behavior and histologic integrity of the detached periosteum after application of the transcrestal hydrodynamic ultrasonic cavitational sinus lift (tHUCSL-INTRALIFT)...
August 2014: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/24351050/periodontal-ligament-derived-stem-cells-exhibit-the-capacity-for-long-term-survival-self-renewal-and-regeneration-of-multiple-tissue-types-in-vivo
#19
Danijela Menicanin, Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Songtao Shi, P Mark Bartold, Stan Gronthos
Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived after 8 weeks post-transplantation and exhibited the capacity to form bone/cementum-like mineralized tissue, ligament structures similar to Sharpey's fibers with an associated vasculature. To evaluate self-renewal potential, PDLSCs were recovered from harvested primary transplants 8 weeks post-transplantation that exhibit an immunophenotype and multipotential capacity comparable to primary PDLSCs...
May 1, 2014: Stem Cells and Development
https://www.readbyqxmd.com/read/24279017/-rotator-cuff-repair-with-decellularized-tendon-slices-for-enhancing-tendon-bone-healing-in-rabbits
#20
Juan Pan, Guoming Liu, Liangju Ning, Jingcong Luo, Fuguo Huang, Tingwu Qin
OBJECTIVE: To investigate the effect of canine decellularized tendon slices (DTSs) on tendon-bone healing in repairing rotator cuff injury of rabbit. METHODS: Canine DTSs were prepared by repetitive freeze/thaw 5 times combined with nuclease processing for 12 hours from the adult Beagles Achilles tendons. Histological observation and cytocompatibility evaluation for the canine DTSs were performed in vitro. Twenty-four mature male New Zealand white rabbits, weighing 2...
September 2013: Chinese Journal of Reparative and Reconstructive Surgery
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