keyword
MENU ▼
Read by QxMD icon Read
search

high throughput

keyword
https://www.readbyqxmd.com/read/28340485/an-approach-for-liposome-immobilization-using-sterically-stabilized-micelles-ssms-as-a-precursor-for-bio-layer-interferometry-based-interaction-studies
#1
Jakob Wallner, Gabriele Lhota, Markus Schosserer, Karola Vorauer-Uhl
Non-fluidic bio-layer interferometry (BLI) has rapidly become a standard tool for monitoring almost all biomolecular interactions in a label-free, real-time and high-throughput manner. High-efficiency screening methods which measure the kinetics of liposomes with a variety of compounds require the immobilization of liposomes. In this work, a method is described for immobilizing liposomes for interaction studies, based on the biophysical principles of this biosensor platform. The immobilization approach includes the loading of DSPE-PEG(2000)-biotin containing sterically stabilized micelles (SSMs) which are restructured in a buffer change step, resulting in an accessible substrate for liposome immobilization...
March 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28340478/different-dna-damage-response-of-cis-and-trans-isomers-of-commonly-used-uv-filter-after-the-exposure-on-adult-human-liver-stem-cells-and-human-lymphoblastoid-cells
#2
Anežka Sharma, Katarína Bányiová, Pavel Babica, Naouale El Yamani, Andrew Richard Collins, Pavel Čupr
2-ethylhexyl 4-methoxycinnamate (EHMC), used in many categories of personal care products (PCPs), is one of the most discussed ultraviolet filters because of its endocrine-disrupting effects. EHMC is unstable in sunlight and can be transformed from trans-EHMC to emergent cis-EHMC. Toxicological studies are focusing only on trans-EHMC; thus the toxicological data for cis-EHMC are missing. In this study, the in vitro genotoxic effects of trans- and cis-EHMC on adult human liver stem cells HL1-hT1 and human-derived lymphoblastoid cells TK-6 using a high-throughput comet assay were studied...
March 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28340440/bioaugmentation-with-hydrolytic-microbes-to-improve-the-anaerobic-biodegradability-of-lignocellulosic-agricultural-residues
#3
P Tsapekos, P G Kougias, S A Vasileiou, L Treu, S Campanaro, G Lyberatos, I Angelidaki
Bioaugmentation with hydrolytic microbes was applied to improve the methane yield of bioreactors fed with agricultural wastes. The efficiency of Clostridium thermocellum and Melioribacter roseus to degrade lignocellulosic matter was evaluated in batch and continuously stirred tank reactors (CSTRs). Results from batch assays showed that C. thermocellum enhanced the methane yield by 34%. A similar increase was recorded in CSTR during the bioaugmentation period; however, at steady-state the effect was noticeably lower (7...
March 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340435/optimization-of-hydrogen-dispersion-in-thermophilic-up-flow-reactors-for-ex-situ-biogas-upgrading
#4
Ilaria Bassani, Panagiotis G Kougias, Laura Treu, Hugo Porté, Stefano Campanaro, Irini Angelidaki
This study evaluates the efficiency of four novel up-flow reactors for ex situ biogas upgrading converting externally provided CO2 and H2 to CH4, via hydrogenotrophic methanogenesis. The gases were injected through stainless steel diffusers combined with alumina ceramic sponge or through alumina ceramic membranes. Pore size, input gas loading and gas recirculation flow rate were modulated to optimize gas-liquid mass transfer, and thus methanation efficiency. Results showed that larger pore size diffusion devices achieved the best kinetics and output-gas quality converting all the injected H2 and CO2, up to 3...
March 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340340/selective-chemical-inhibition-of-pgc-1%C3%AE-gluconeogenic-activity-ameliorates-type-2-diabetes
#5
Kfir Sharabi, Hua Lin, Clint D J Tavares, John E Dominy, Joao Paulo Camporez, Rachel J Perry, Roger Schilling, Amy K Rines, Jaemin Lee, Marc Hickey, Melissa Bennion, Michelle Palmer, Partha P Nag, Joshua A Bittker, José Perez, Mark P Jedrychowski, Umut Ozcan, Steve P Gygi, Theodore M Kamenecka, Gerald I Shulman, Stuart L Schreiber, Patrick R Griffin, Pere Puigserver
Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to inhibit hepatic gluconeogenesis, could be potentially used for treatment of T2D. Thus, we designed a high-throughput chemical screen platform to quantify PGC-1α acetylation in cells and identified small molecules that increase PGC-1α acetylation, suppress gluconeogenic gene expression, and reduce glucose production in hepatocytes...
March 23, 2017: Cell
https://www.readbyqxmd.com/read/28339688/an-activity-staining-method-on-filtration-paper-enables-high-throughput-screening-of-temperature-sensitive-and-inactive-mutations-of-rice-%C3%AE-amylase-for-improvement-of-rice-grain-quality
#6
Hiromoto Yamakawa, Rieko Hirai-Kimura, Yuriko Nakata, Masaru Nakata, Masaharu Kuroda, Takeshi Yamaguchi
α-Amylase is a starch-hydrolyzing enzyme (EC 3.2.1.1) indispensable for germination of cereal seeds, but it is also expressed during the ripening stage. Previous studies demonstrated that the enzyme is activated in developing rice seeds under extremely hot weather and triggers a loss of grain quality by hindering the accumulation of storage starch in the endosperm. Since inactive or, preferably, heat-labile α-amylases are preferable for breeding premium rice, we developed a method for rapid screening of inactive and temperature-sensitive mutants of the enzyme by combining the random mutagenesis by error-prone PCR and an on-filter activity test of the recombinant enzyme expressed by Escherichia coli...
February 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28339484/a-high-throughput-qpcr-system-for-simultaneous-quantitative-detection-of-dairy-lactococcus-lactis-and-leuconostoc-bacteriophages
#7
Musemma K Muhammed, Lukasz Krych, Dennis S Nielsen, Finn K Vogensen
Simultaneous quantitative detection of Lactococcus (Lc.) lactis and Leuconostoc species bacteriophages (phages) has not been reported in dairies using undefined mixed-strain DL-starters, probably due to the lack of applicable methods. We optimized a high-throughput qPCR system that allows simultaneous quantitative detection of Lc. lactis 936 (now SK1virus), P335, c2 (now C2virus) and Leuconostoc phage groups. Component assays are designed to have high efficiencies and nearly the same dynamic detection ranges, i...
2017: PloS One
https://www.readbyqxmd.com/read/28339216/embarking-on-a-chemical-space-odyssey
#8
Shiao Y Chow, Adam Nelson
The chemical space explored in drug discovery programs is restricted by a narrow reaction toolkit and the frequent failure of even these reactions with polar and functionalized substrates. Recently, high-throughput reaction optimization has been integrated into discovery workflows, thereby increasing the value of specific reaction classes in the toolkit. It is likely that high-throughput experimentation will enable expansion of the synthetic chemistry that is widely exploited in discovery, thereby increasing innovation in medicinal chemistry...
March 24, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28339201/high-throughput-in-silico-structure-validation-and-revision-of-halogenated-natural-products-is-enabled-by-parametric-corrections-to-dft-computed-13-c-nmr-chemical-shifts-and-spin-spin-coupling-constants
#9
Andrei G Kutateladze, D Sai Reddy
Halogenated natural products constitute diverse and promising feedstock for molecular pharmaceuticals. However, their solution-structure elucidation by NMR presents several challenges, including the lack of fast methods to compute (13)C chemical shifts for carbons bearing heavy atoms. We show that parametric corrections to DFT-computed chemical shifts in conjunction with rff-computed spin-spin coupling constants allow for fast and reliable screening of a large number of reported halogenated natural products, resulting in expedient structure validation or revision...
March 24, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28338930/expath-tool-a-system-for-comprehensively-analyzing-regulatory-pathways-and-coexpression-networks-from-high-throughput-transcriptome-data
#10
Han-Qin Zheng, Nai-Yun Wu, Chi-Nga Chow, Kuan-Chieh Tseng, Chia-Hung Chien, Yu-Cheng Hung, Guan-Zhen Li, Wen-Chi Chang
Next generation sequencing (NGS) has become the mainstream approach for monitoring gene expression levels in parallel with various experimental treatments. Unfortunately, there is no systematical webserver to comprehensively perform further analysis based on the huge amount of preliminary data that is obtained after finishing the process of gene annotation. Therefore, a user-friendly and effective system is required to mine important genes and regulatory pathways under specific conditions from high-throughput transcriptome data...
March 13, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28338722/dissecting-the-transcriptional-patterns-of-social-dominance-across-teleosts
#11
Suzy C P Renn, Cynthia F O'Rourke, Nadia Aubin-Horth, Eleanor J Fraser, Hans A Hofmann
In many species, under varying ecological conditions, social interactions among individuals result in the formation of dominance hierarchies. Despite general similarities, there are robust differences among dominance hierarchies across species, populations, environments, life stages, sexes, and individuals. Understanding the proximate mechanisms underlying the variation is an important step toward understanding the evolution of social behavior. However, physiological changes associated with dominance, such as gonadal maturation and somatic growth, often complicate efforts to identify the specific underlying mechanisms...
December 16, 2016: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/28338236/hsa-mir-3199-2-and-hsa-mir-1293-as-novel-prognostic-biomarkers-of-papillary-renal-cell-carcinoma-by-cox-ratio-risk-regression-model-screening
#12
Wen Luo, Lei Wang, Mao-Hua Luo, Yu-Zhu Huang, Hua Yang, Yu Zhou, Hong-Tao Jia, Xiu-Xin Wang
Papillary renal cell carcinoma(PRCC) is the second most common and aggressive renal cell carcinoma. Identification of novel microRNA biomarkers could be beneficial for the diagnosis and prognosis of PRCCpatients.We aimed to screen differentially expressed miRNAs that can act as prognostic factors and to predict the survival of PRCC patients. High-throughput data of miRNAs of 274 PRCC samples were downloaded from TCGA(The Cancer Genome Atlas) dataset and interested miRNAs were identified. Hierarchical clustering and principal component analysis (PCA) were performed on these miRNAs...
March 24, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28338080/bivariate-genomic-analysis-identifies-a-hidden-locus-associated-with-bacteria-hypersensitive-response-in-arabidopsis-thaliana
#13
Biao Wang, Zhuocheng Li, Weilin Xu, Xiao Feng, Qianhui Wan, Yanjun Zan, Sitong Sheng, Xia Shen
Multi-phenotype analysis has drawn increasing attention to high-throughput genomic studies, whereas only a few applications have justified the use of multivariate techniques. We applied a recently developed multi-trait analysis method on a small set of bacteria hypersensitive response phenotypes and identified a single novel locus missed by conventional single-trait genome-wide association studies. The detected locus harbors a minor allele that elevates the risk of leaf collapse response to the injection of avrRpm1-modified Pseudomonas syringae (P = 1...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28338079/dissecting-mirna-gene-repression-on-single-cell-level-with-an-advanced-fluorescent-reporter-system
#14
Nicolas Lemus-Diaz, Kai O Böker, Ignacio Rodriguez-Polo, Michael Mitter, Jasmin Preis, Maximilian Arlt, Jens Gruber
Despite major advances on miRNA profiling and target predictions, functional readouts for endogenous miRNAs are limited and frequently lead to contradicting conclusions. Numerous approaches including functional high-throughput and miRISC complex evaluations suggest that the functional miRNAome differs from the predictions based on quantitative sRNA profiling. To resolve the apparent contradiction of expression versus function, we generated and applied a fluorescence reporter gene assay enabling single cell analysis...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337937/evaluation-of-methanogenic-microbial-electrolysis-cells-mecs-under-closed-open-circuit-operations
#15
Liwen Luo, Suyun Xu, Yueqing Jin, Runqi Han, Hongbo Liu, Fan Lü
This study investigated the independent roles of biofilm and external power supply for volatile fatty acids (VFAs) degradation and methane production in the microbial electrolysis cell (MEC). Reactors were operated in fed-batch mode in the presence of graphite felt(GF) or titanium-rod (Ti) as electrodes, in open circuit (OC, without applied voltage) or closed circuit (CC) conditions, i.e. R1 (Ti+CC), R2 (GF+CC) and R3 (GF+OC). The first-order kinetic analysis of acetate degradation indicated that the presence of GF biofilm and applied voltage in R2 improved the acetate degradation rate by 23% as compared to R1, while it was only 7% increment in R3 with GF biofilm...
March 24, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28337850/high-throughput-screening-of-vascular-endothelium-destructive-or-protective-microenvironments-cooperative-actions-of-extracellular-matrix-composition-stiffness-and-structure
#16
Yonghui Ding, Michael Floren, Wei Tan
Pathological modification of the subendothelial extracellular matrix (ECM) has closely been associated with endothelial activation and subsequent cardiovascular disease progression. To understand regulatory mechanisms of these matrix modifications, the majority of previous efforts have focused on the modulation of either chemical composition or matrix stiffness on 2D smooth surfaces without simultaneously probing their cooperative effects on endothelium function on in vivo like 3D fibrous matrices. To this end, a high-throughput, combinatorial microarray platform on 2D and 3D hydrogel settings to resemble the compositions, stiffness, and structure of healthy and diseased subendothelial ECM has been established, and further their respective and combined effects on endothelial attachment, proliferation, inflammation, and junctional integrity have been investigated...
March 24, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28337517/a-column-switching-ultrahigh-performance-liquid-chromatography-tandem-mass-spectrometry-method-to-determine-anandamide-and-2-arachidonoylglycerol-in-plasma-samples
#17
Camila Marchioni, Israel Donizeti de Souza, Caroline Fernandes Grecco, José Alexandre Crippa, Vitor Tumas, Maria Eugênia Costa Queiroz
This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm × 4 mm × 25 μM) in the first dimension and a core-shell Kinetex C18 (100 mm × 2, 1.7 mm × 1 μM) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode...
March 23, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28337455/analysis-of-the-bacterial-communities-in-two-liquors-of-soy-sauce-aroma-as-revealed-by-high-throughput-sequencing-of-the-16s-rrna-v4-hypervariable-region
#18
Jing Tang, Xiaoxin Tang, Ming Tang, Ximin Zhang, Xiaorong Xu, Yin Yi
Chinese liquor is one of the world's oldest distilled alcoholic beverages and an important commercial fermented product in China. The Chinese liquor fermentation process has three stages: making Daqu (the starter), stacking fermentation on the ground, and liquor fermentation in pits. We investigated the bacterial diversity of Maotai and Guotai Daqu and liquor fermentation using high-throughput sequencing of the V4 hypervariable region of the 16S rRNA gene. A total of 70,297 sequences were obtained from the Daqu samples and clustered into 17 phyla...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28337392/microsatellite-markers-for-population-studies-of-the-salt-marsh-species-juncus-roemerianus-juncaceae
#19
Hayley R Tumas, Brian M Shamblin, Mark S Woodrey, Campbell J Nairn
PREMISE OF THE STUDY: Juncus roemerianus (Juncaceae) is a foundational species and ecosystem engineer of salt marshes in the Gulf of Mexico. These ecosystems provide coastal flood attenuation, nurseries for important species, and other ecosystem services, but are experiencing significant decline. Nuclear microsatellite markers were developed for J. roemerianus to study genetic diversity and population structure for conservation and restoration efforts. METHODS AND RESULTS: Illumina NextSeq high-throughput sequencing was used to develop a panel of 19 polymorphic microsatellite markers that were tested across individuals from three populations on the Gulf Coast...
March 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28336965/exploring-approaches-for-detecting-protein-functional-similarity-within-an-orthology-based-framework
#20
Christian X Weichenberger, Antonia Palermo, Peter P Pramstaller, Francisco S Domingues
Protein functional similarity based on gene ontology (GO) annotations serves as a powerful tool when comparing proteins on a functional level in applications such as protein-protein interaction prediction, gene prioritization, and disease gene discovery. Functional similarity (FS) is usually quantified by combining the GO hierarchy with an annotation corpus that links genes and gene products to GO terms. One large group of algorithms involves calculation of GO term semantic similarity (SS) between all the terms annotating the two proteins, followed by a second step, described as "mixing strategy", which involves combining the SS values to yield the final FS value...
March 23, 2017: Scientific Reports
keyword
keyword
39332
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"