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https://www.readbyqxmd.com/read/29792945/cross-linking-by-epichlorohydrin-and-diepoxybutane-correlates-with-cytotoxicity-and-leads-to-apoptosis-in-human-leukemia-hl-60-cells
#1
Phuong M Le, Vanesa L Silvestri, Samuel C Redstone, Jordanne B Dunn, Julie T Millard
The bifunctional alkylating agents epichlorohydrin (ECH) and diepoxybutane (DEB) have been linked to increased cancer risks in industrial workers. These compounds react with DNA and proteins, leading to genotoxic effects. We used the comet assay to monitor formation of cross-links in HL-60 cells treated with ECH, DEB, and the structurally related anti-cancer drug mechlorethamine (HN2). We report a time- and dose-dependent cytotoxicity that correlated with cross-linking activity, following the order HN2 > DEB > ECH...
May 21, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29687868/epigallocatechin-gallate-inhibits-the-proliferation-and-induces-apoptosis-of-multiple-myeloma-cells-via-inactivating-ezh2
#2
C-G Zhou, L-M Hui, J-M Luo
OBJECTIVE: Epigallocatechin gallate (EGCG), the major chemical constituent of green tea, exhibits remarkable anti-tumor effect properties. In the present work, we aim to explore the effect and underlying mechanism of EGCG on multiple myeloma (MM) cells. MATERIALS AND METHODS: The effects of EGCG on MM cells proliferation and apoptosis were determined by CCK-8 assay and flow cytometry assay. The siRNAs were used to inhibit endogenous expression of EZH2. Enforced expression of EZH2 in U266 cells was accomplished by transfecting EZH2 plasmid...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29670153/bri1-and-bak1-interact-with-g-proteins-and-regulate-sugar-responsive-growth-and-development-in-arabidopsis
#3
Yuancheng Peng, Liangliang Chen, Shengjun Li, Yueying Zhang, Ran Xu, Zupei Liu, Wuxia Liu, Jingjing Kong, Xiahe Huang, Yingchun Wang, Beijiu Cheng, Leiying Zheng, Yunhai Li
Sugars function as signal molecules to regulate growth, development, and gene expression in plants, yeasts, and animals. A coordination of sugar availability with phytohormone signals is crucial for plant growth and development. The molecular link between sugar availability and hormone-dependent plant growth are largely unknown. Here we report that BRI1 and BAK1 are involved in sugar-responsive growth and development. Glucose influences the physical interactions and phosphorylations of BRI1 and BAK1 in a concentration-dependent manner...
April 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29665895/-inhibitory-effect-of-mir-125b-down-regulation-on-proliferation-of-leukemia-cell-k562
#4
Jie Liu, Chang-Qing Tong
OBJECTIVE: To explore inhibitory effect of miR-125b down-regulation on proliferation of leukemia cell K562. METHODS: miR-125b inhibitor and miR-125b NC were transfected into K562 cells by liposome LipofectamineTM 2000, the cell viability was measured by MTT assay, cell cloning ability was detected by agarose cloning assay, cell cycle was measured by flow cytometry. The expression of BCL-2, BCL-2 homology antagonist/liller 1(BAK1), p53 and p53 up-regulated modulator of apoptosis (Puma) was measured by Western blot...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29581862/doxorubicin-induced-loss-of-dna-topoisomerase-ii-and-dnmt1-dependent-suppression-of-mir-125b-induces-chemoresistance-in-alk-positive-cells
#5
Annabelle Congras, Nina Caillet, Nouritza Torossian, Cathy Quelen, Camille Daugrois, Pierre Brousset, Laurence Lamant, Fabienne Meggetto, Coralie Hoareau-Aveilla
Systemic anaplastic large-cell lymphoma (ALCL) is a childhood T cell neoplasm defined by the presence or absence of translocations that lead to the ectopic expression of anaplastic lymphoma kinase (ALK), with nucleophosmin-ALK (NPM-ALK) fusions being the most common. Polychemotherapy involving doxorubicin is the standard first-line treatment but for the 25 to 35% of patients who relapse and develop resistance the prognosis remains poor. We studied the potential role of the microRNA miR-125b in the development of resistance to doxorubicin in NPM-ALK(+) ALCL...
March 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29504848/signalling-pathways-identified-in-salivary-glands-from-primary-sj%C3%A3-gren-s-syndrome-patients-reveal-enhanced-adipose-tissue-development
#6
Lara A Aqrawi, Janicke Liaaen Jensen, Gunnvor Øijordsbakken, Ann-Kristin Ruus, Ståle Nygård, Marit Holden, Roland Jonsson, Hilde Kanli Galtung, Kathrine Skarstein
A characteristic feature of primary Sjögren's syndrome (pSS) is the destruction of salivary and lacrimal glands mediated by mononuclear cell infiltration. Adipocytes can also occupy a large portion of the salivary gland (SG) tissue area, although little is known about their significance in pSS. We have previously investigated adipose tissue infiltration in SG biopsies from pSS patients and non-SS sicca controls. Our findings indicated the distinct incidence of adipose tissue replacement in pSS patients, where adipocytes were detected in interleukin (IL) 6 rich regions...
May 2018: Autoimmunity
https://www.readbyqxmd.com/read/29472693/a-new-allele-of-the-brachytic2-gene-in-maize-can-efficiently-modify-plant-architecture
#7
Lai Wei, Xuan Zhang, Zhihai Zhang, Huanhuan Liu, Zhongwei Lin
The applications of semi-dwarf genes such as sd1 and Rht1 in rice and wheat resulted in the first "green revolution" in the 1960s. However, such semi-dwarf genes that can efficiently reduce plant stature and have few negative yield traits have not yet been identified in maize. In this study, a new allele of Brachytic2 gene (qpa1) encoding P-glycoprotein was rapidly fine-mapped using a modified method. The qpa1, containing a 241-bp deletion in the last exon, had no negative effect on yield, but greatly modified the plant architecture including significantly reduced plant height and ear height, increased stalk diameter and erected leaf...
February 23, 2018: Heredity
https://www.readbyqxmd.com/read/29463771/stress-induced-factor-2-a-leucine-rich-repeat-kinase-regulates-basal-plant-pathogen-defense
#8
Ning Yuan, Shuangrong Yuan, Zhigang Li, Man Zhou, Peipei Wu, Qian Hu, Venugopal Mendu, Liangjiang Wang, Hong Luo
Protein kinases play fundamental roles in plant development and environmental stress responses. Here, we identified the STRESS INDUCED FACTOR ( SIF ) gene family, which encodes four leucine-rich repeat receptor-like protein kinases in Arabidopsis ( Arabidopsis thaliana ). The four genes, SIF1 to SIF4 , are clustered in the genome and highly conserved, but they have temporally and spatially distinct expression patterns. We employed Arabidopsis SIF knockout mutants and overexpression transgenics to examine SIF involvement during plant pathogen defense...
April 2018: Plant Physiology
https://www.readbyqxmd.com/read/29442188/damage-regulated-autophagy-modulator-1-in-oral-inflammation-and-infection
#9
Svenja Memmert, A V B Nogueira, A Damanaki, M Nokhbehsaim, S Eick, T Divnic-Resnik, A Spahr, B Rath-Deschner, A Till, W Götz, J A Cirelli, A Jäger, J Deschner
OBJECTIVES: Damage-regulated autophagy modulator (DRAM) 1 is a p53 target gene with possible involvement in oral inflammation and infection. This study sought to examine the presence and regulation of DRAM1 in periodontal diseases. MATERIAL AND METHODS: In vitro, human periodontal ligament fibroblasts were exposed to interleukin (IL)-1β and Fusobacterium nucleatum for up to 2 days. The DRAM1 synthesis and its regulation were analyzed by real-time PCR, immunocytochemistry, and ELISA...
February 13, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29426870/leucine-rich-repeat-receptor-like-gene-screen-reveals-that-nicotiana-rxeg1-regulates-glycoside-hydrolase-12-mamp-detection
#10
Yan Wang, Yuanpeng Xu, Yujing Sun, Huibin Wang, Jiaming Qi, Bowen Wan, Wenwu Ye, Yachun Lin, Yuanyuan Shao, Suomeng Dong, Brett M Tyler, Yuanchao Wang
Activation of innate immunity by membrane-localized receptors is conserved across eukaryotes. Plant genomes contain hundreds of such receptor-like genes and those encoding proteins with an extracellular leucine-rich repeat (LRR) domain represent the largest family. Here, we develop a high-throughput approach to study LRR receptor-like genes on a genome-wide scale. In total, 257 tobacco rattle virus-based constructs are generated to target 386 of the 403 identified LRR receptor-like genes in Nicotiana benthamiana for silencing...
February 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29409480/contrasting-roles-of-h3k4me3-and-h3k9me3-in-regulation-of-apoptosis-and-gemcitabine-resistance-in-human-pancreatic-cancer-cells
#11
Chunwan Lu, Dafeng Yang, Maria E Sabbatini, Aaron H Colby, Mark W Grinstaff, Nicholas H Oberlies, Cedric Pearce, Kebin Liu
BACKGROUND: Pancreas ductal adenocarcinoma (PDAC) has the most dismal prognosis among all human cancers since it is highly resistant to chemotherapy, radiotherapy and immunotherapy. The anticipated consequence of all therapies is induction of tumor apoptosis. The highly resistance nature of PDACs to all therapies suggests that the intrinsic tumor cell factors, likely the deregulated apoptosis pathway, are key mechanisms underlying PDAC non-response to these therapies, rather than the therapeutic agents themselves...
February 6, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29371249/a-poly-a-ribonuclease-controls-the-cellotriose-based-interaction-between-piriformospora-indica-and-its-host-arabidopsis
#12
Joy M Johnson, Johannes Thürich, Elena K Petutschnig, Lothar Altschmied, Doreen Meichsner, Irena Sherameti, Julian Dindas, Anna Mrozinska, Christian Paetz, Sandra S Scholz, Alexandra C U Furch, Volker Lipka, Rainer Hedrich, Bernd Schneider, Aleš Svatoš, Ralf Oelmüller
Piriformospora indica , an endophytic root-colonizing fungus, efficiently promotes plant growth and induces resistance to abiotic stress and biotic diseases. P. indica fungal cell wall extract induces cytoplasmic calcium elevation in host plant roots. Here, we show that cellotriose (CT) is an elicitor-active cell wall moiety released by P. indica into the medium. CT induces a mild defense-like response, including the production of reactive oxygen species, changes in membrane potential, and the expression of genes involved in growth regulation and root development...
March 2018: Plant Physiology
https://www.readbyqxmd.com/read/29368980/tp53-is-required-for-becn1-and-atg5-dependent-cell-death-induced-by-sphingosine-kinase-1-inhibition
#13
Santiago Lima, Kazuaki Takabe, Jason Newton, Kumar Saurabh, Megan M Young, Andreia Machado Leopoldino, Nitai C Hait, Jane L Roberts, Hong-Gang Wang, Paul Dent, Sheldon Milstien, Laurence Booth, Sarah Spiegel
The bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) and the enzyme that produces it, SPHK1 (sphingosine kinase 1), regulate many processes important for the etiology of cancer. It has been suggested that SPHK1 levels are regulated by the tumor suppressor protein TP53, a key regulator of cell cycle arrest, apoptosis, and macroautophagy/autophagy. However, little is still known of the relationship between TP53 and SPHK1 activity in the regulation of these processes. To explore this link, we examined the effects of inhibiting SPHK1 in wild-type and TP53 null cancer cell lines...
January 25, 2018: Autophagy
https://www.readbyqxmd.com/read/29361797/biochemical-analysis-of-the-role-of-leucine-rich-repeat-receptor-like-kinases-and-the-carboxy-terminus-of-receptor-kinases-in-regulating-kinase-activity-in-arabidopsis-thaliana-and-brassica-oleracea
#14
Eun-Seok Oh, Yeon Lee, Won Byoung Chae, Jana Jeevan Rameneni, Yong-Soon Park, Yong Pyo Lim, Man-Ho Oh
Protein post-translational modification by phosphorylation is essential for the activity and stability of proteins in higher plants and underlies their responses to diverse stimuli. There are more than 300 leucine-rich repeat receptor-like kinases (LRR-RLKs), a major group of receptor-like kinases (RLKs) that plays an important role in growth, development, and biotic stress responses in higher plants. To analyze auto- and transphosphorylation patterns and kinase activities in vitro, 43 full-length complementary DNA (cDNA) sequences were cloned from genes encoding LRR-RLKs...
January 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29299204/downregulation-of-microrna-125a-is-involved-in-intervertebral-disc-degeneration-by-targeting-pro-apoptotic-bcl-2-antagonist-killer-1
#15
Ping Liu, Feng Chang, Ting Zhang, Gang Gao, Chen Yu, Sheng-Qiang Ding, Gen-Le Zuo, Xin-Hu Huang
Objectives: To investigate the role of the microRNA-125a (miR-125a) and BAK1 in intervertebral disc degeneration (IDD). Materials and Methods: Degenerative lumbar nucleus pulposus (NP) tissues were obtained from 193 patients who underwent resection, and normal controls consisted of normal NP tissues from 32 patients with traumatic lumbar fracture in our hospital. All patients were graded according to the Pfirrmann criteria. QRT-PCR was used to detect the expression of miR-125a and BAK1, and apoptosis of NP tissues detected by TUNEL staining...
November 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29296753/an-aptamer-based-targeted-delivery-of-mir-26a-protects-mice-against-chemotherapy-toxicity-while-suppressing-tumor-growth
#16
Toshihiko Tanno, Peng Zhang, Christopher A Lazarski, Yang Liu, Pan Zheng
The efficacy of traditional chemotherapy is limited by its toxicity, especially with regard to hematopoiesis. Here we show that miR-26a plays a critical role in protecting mice against chemotherapy-induced myeloid suppression by targeting a proapoptotic protein (Bak1) in hematopoietic stem/progenitor cells (HSPCs). Because c-Kit is expressed at high levels in HSPCs, we designed a microRNA-aptamer chimera that contains miR-26a mimic and c-Kit-targeting aptamer and successfully delivered miR-26a into HSPCs to attenuate toxicity of 5' fluorouracil (5-FU) and carboplatin...
June 27, 2017: Blood Advances
https://www.readbyqxmd.com/read/29288738/vacuole-integrity-maintained-by-duf300-proteins-is-required-for-brassinosteroid-signaling-regulation
#17
Qinsong Liu, Thomas Vain, Corrado Viotti, Siamsa M Doyle, Danuše Tarkowská, Ondřej Novák, Cyril Zipfel, Folke Sitbon, Stéphanie Robert, Daniel Hofius
Brassinosteroid (BR) hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1) together with co-receptors such as BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1). BRI1 abundance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remains elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 (LAZ1) and LAZ1 HOMOLOG1 (LAZ1H1), causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling...
April 2, 2018: Molecular Plant
https://www.readbyqxmd.com/read/29234488/sirt6-histone-deacetylase-functions-as-a-potential-oncogene-in-human-melanoma
#18
Liz Mariely Garcia-Peterson, Mary Ann Ndiaye, Chandra K Singh, Gagan Chhabra, Wei Huang, Nihal Ahmad
Melanoma is an aggressive skin cancer that can rapidly metastasize to become fatal, if not diagnosed early. Despite recent therapeutic advances, management of melanoma remains difficult. Therefore, novel molecular targets and strategies are required to manage this neoplasm. This study was undertaken to determine the role of the sirtuin SIRT6 in melanoma. Employing a panel of human melanoma cells and normal human melanocytes, we found significant SIRT6 mRNA and protein upregulation in melanoma cells. Further, using a tissue microarray coupled with quantitative Vectra analysis, we demonstrated significant SIRT6 overexpression in human melanoma tissues...
September 2017: Genes & Cancer
https://www.readbyqxmd.com/read/29231977/dids-inhibits-overexpression-bak1-induced-mitochondrial-apoptosis-through-gsk3%C3%AE-%C3%AE-catenin-signaling-pathway
#19
Xiayun Yang, Shusheng Tang, Daowen Li, Xiaohong Yu, Fuyun Wang, Xilong Xiao
Bcl-2 homologous antagonist/killer (BAK1) is a critical regulator of mitochondrial apoptosis. Although upregulation of BAK1 induces apoptosis has been established, the underlying molecular mechanism is far from clear. 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), an organic anion used as a blocker of anion exchangers and chloride channels, has been proved to rescue cell apoptosis both in vitro and in vivo. However, whether DIDS can inhibit BAK1-induced mitochondrial apoptosis remains undefined. Thus, this study aimed to explore whether DIDS could protect BAK1-induced apoptosis through GSK3β/β-catenin signaling pathway...
June 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29211989/allosteric-control-of-a-plant-receptor-kinase-through-s-glutathionylation
#20
Alexander S Moffett, Kyle W Bender, Steven C Huber, Diwakar Shukla
Growing evidence supports the importance of protein S-glutathionylation as a regulatory post-translational modification with functional consequences for proteins. Discoveries of redox-state-dependent protein kinase S-glutathionylation have fueled discussion of redox-sensitive signaling. Following previously published experimental evidence for S-glutathionylation induced deactivation of the Arabidopsis thaliana kinase BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR-LIKE KINASE 1 (BAK1), we investigated the consequences of S-glutathionylation on the equilibrium conformational ensemble of BAK1 using all-atom molecular dynamics simulations...
December 5, 2017: Biophysical Journal
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