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Hong Zhu, Wei Xia, Xing-Bo Mo, Xiang Lin, Ying-Hua Qiu, Neng-Jun Yi, Yong-Hong Zhang, Fei-Yan Deng, Shu-Feng Lei
OBJECTIVE: Rheumatoid arthritis (RA) is a complex autoimmune disease. Using a gene-based association research strategy, the present study aims to detect unknown susceptibility to RA and to address the ethnic differences in genetic susceptibility to RA between European and Asian populations. METHODS: Gene-based association analyses were performed with KGG 2.5 by using publicly available large RA datasets (14,361 RA cases and 43,923 controls of European subjects, 4,873 RA cases and 17,642 controls of Asian Subjects)...
2016: PloS One
Kohji Yamada, Yusuke Saijo, Hirofumi Nakagami, Yoshitaka Takano
Microbial pathogens strategically acquire metabolites from their hosts during infection. Here we show that the host can intervene to prevent such metabolite loss to pathogens. Phosphorylation-dependent regulation of sugar transporter 13 (STP13) is required for antibacterial defense in the plant Arabidopsis thaliana STP13 physically associates with the flagellin receptor flagellin-sensitive 2 (FLS2) and its co-receptor BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1). BAK1 phosphorylates STP13 at threonine 485, which enhances its monosaccharide uptake activity to compete with bacteria for extracellular sugars...
November 24, 2016: Science
Koste Abdissa Yadeta, James Mitch Elmore, Athena Y Creer, Baomin Feng, Jessica Y Franco, Jose Sebastian Rufian, Ping He, Brett S Phinney, Gitta Laurel Coaker
Membrane localized proteins perceive and respond to biotic and abiotic stresses. We performed quantitative proteomics on plasma membrane-enriched samples from Arabidopsis treated with bacterial flagellin. We identified multiple receptor-like protein kinases (RLKs) changing in abundance, including cysteine-rich receptor-like kinases (CRKs) that are upregulated upon perception of flagellin. CRKs possess extracellular cysteine-rich domains and comprise a gene family consisting of 46 members in Arabidopsis. Single T-DNA insertion lines in CRK28 and CRK29, two CRKs induced in response to flagellin perception, did not exhibit robust alterations in immune responses...
November 16, 2016: Plant Physiology
Michael Kleemann, Jeremias Bereuther, Simon Fischer, Kim Marquart, Simon Hänle, Kristian Unger, Verena Jendrossek, Christian U Riedel, René Handrick, Kerstin Otte
The development and progression of cancer can be ascribed to imbalances in gene regulation leading to aberrant cellular behavior. The loss of micro RNAs (miRNAs) exhibiting tumor-suppressive function has been demonstrated to be often causative for uncontrolled cell proliferation, migration or tissue infiltration. The installation of de novo tumor suppressive function by using pro-apoptotic miRNAs might be a promising therapeutic approach. In addition, there is a great demand for novel biomarkers for the prognosis of cancer, which prompted us to transfer a high content miRNA screening initially performed to identify bioprocess relevant miRNAs in Chinese hamster ovary (CHO) cells to human cancer cell lines ...
November 4, 2016: Oncotarget
Dominik Klauser, Pascale Flury, Thomas Boller, Sebastian Merker
The membrane-based receptor-like kinase BAK1 has been reported to interact with a number of other membrane-based receptors to contribute to a variety of signaling responses to exogenous and endogenous cues. These include brassinosteroid hormones as well as conserved microbe-derived and endogenous patterns. More recently, several lines of evidence have been reported to expand this concept also to the detection and deterrence of insect herbivores. We hereby present results that further support this hypothesis as they show that in Arabidopsis thaliana, herbivore oral secretions trigger two hallmark responses of plant innate immunity and that these responses are significantly reduced in plants that lack functional BAK1 receptors...
October 28, 2016: Plant Signaling & Behavior
V Paunovic, M Kosic, S Djordjevic, A Zugic, N Djalinac, U Gasic, V Trajkovic, J Harhaji-Trajkovic
Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27...
September 30, 2016: Cellular and Molecular Biology
Lei Li, Panya Kim, Liping Yu, Gaihong Cai, She Chen, James R Alfano, Jian-Min Zhou
The Arabidopsis immune receptor FLS2 and co-receptor BAK1 perceive the bacterial flagellin epitope flg22 to activate plant immunity. To prevent this response, phytopathogenic bacteria deploy a repertoire of effector proteins to perturb immune signaling. However, the effector-induced perturbation is often sensed by the host, triggering another layer of immunity. We report that the Pseudomonas syringae effector HopB1 acts as a protease to cleave immune-activated BAK1. Prior to activation, HopB1 constitutively interacts with FLS2...
October 12, 2016: Cell Host & Microbe
He Ma, Xiaohui Wang, Tuanzhu Ha, Ming Gao, Li Liu, Ruitao Wang, Kaijiang Yu, John H Kalbfleisch, Race L Kao, David L Williams, Chuanfu Li
BACKGROUND:  This study examined the effect of miR-125b on sepsis-induced cardiac dysfunction. METHODS:  Mouse hearts were transfected with lentivirus expressing miR-125b (LmiR-125b) seven days prior to cecal ligation and puncture (CLP) induced sepsis. Cardiac function was examined by echocardiography before and 6 hours after CLP (n=6/group). Survival outcome was monitored following CLP sepsis (n=12/group). RESULTS:  LmiR-125b transfection significantly attenuated CLP sepsis-induced cardiac dysfunction...
September 28, 2016: Journal of Infectious Diseases
Tao Chen, Kai Bi, Zhangchao He, Zhixiao Gao, Ying Zhao, Yanping Fu, Jiasen Cheng, Jiatao Xie, Daohong Jiang
Botrytis-induced kinase1 (BIK1), a receptor-like cytoplasmic kinase, plays an important role in resistance against pathogens and insects in Arabidopsis thaliana. However, it remains unknown whether BIK1 functions against Plasmodiophora brassicae, an obligate biotrophic protist that attacks cruciferous plants and induces gall formation on roots. Here, we investigated the potential roles of receptors FLS2, BAK1, and BIK1 in the infection of P. brassicae cruciferous plants. Wild-type plants, fls2, and bak1 mutants showed typical symptom on roots, and the galls were filled with large quantities of resting spores, while bik1 mutant plants exhibited strong resistance to P...
2016: Frontiers in Physiology
Malick Mbengue, Gildas Bourdais, Fabio Gervasi, Martina Beck, Ji Zhou, Thomas Spallek, Sebastian Bartels, Thomas Boller, Takashi Ueda, Hannah Kuhn, Silke Robatzek
Sensing of potential pathogenic bacteria is of critical importance for immunity. In plants, this involves plasma membrane-resident pattern recognition receptors, one of which is the FLAGELLIN SENSING 2 (FLS2) receptor kinase. Ligand-activated FLS2 receptors are internalized into endosomes. However, the extent to which these spatiotemporal dynamics are generally present among pattern recognition receptors (PRRs) and their regulation remain elusive. Using live-cell imaging, we show that at least three other receptor kinases associated with plant immunity, PEP RECEPTOR 1/2 (PEPR1/2) and EF-TU RECEPTOR (EFR), internalize in a ligand-specific manner...
September 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
Nirpesh Adhikari, Sanjiv Neupane, Gi-Jeong Gwon, Ji-Youn Kim, Chang-Hyeon An, Sanggyu Lee, Wern-Joo Sohn, Youngkyun Lee, Jae-Young Kim
Grainyhead-like 3 (Grhl3) is a transcription factor involved in epithelial morphogenesis. In the present study, we evaluated the developmental role of Grhl3 in structural formation of the circumvallate papilla (CVP), which undergoes dynamic morphological changes during organogenesis. The specific expression pattern of Grhl3 was examined in the CVP-forming region, specifically in the apex and epithelial stalk from E13.5 to E15.5 using in situ hybridization. To determine the role of Grhl3 in epithelial morphogenesis of the CVP, we employed an in vitro tongue culture method, wherein E13...
September 1, 2016: Histochemistry and Cell Biology
Li Kong, Bo Liu, Chenghong Zhang, Bing Wang, Hongfei Wang, Xiaoxia Song, Yang Yang, Xiang Ren, Liangwei Yin, Hui Kong, Haiying Ma
Oxidative stress due to excessive light exposure can exacerbate a variety of human retinal diseases by accelerating photoreceptor cell death. The thioredoxin (Trx) system is considered to play a crucial role in reduction/oxidation (redox) regulation of signal transduction and in cell defense against oxidative stresses. Sulforaphane (SF) protects cells from oxidative damage through nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which is responsible for multiple detoxification processes, including elevating the expression of Trx...
August 25, 2016: Neurochemistry International
Masoumeh Falah, Mohammad Najafi, Massoud Houshmand, Mohammad Farhadi
Age-related hearing impairment (ARHI) is a progressive and a common sensory disorder in the elderly and will become an increasingly important clinical problem given the growing elderly population. Apoptosis of cochlear cells is an important factor in animal models of ARHI. As these cells cannot regenerate, their loss leads to irreversible hearing impairment. Identification of molecular mechanisms can facilitate disease prevention and effective treatment. In this study, we compared the expression of the genes BAK1 and BCL2 as two arms of the intrinsic apoptosis pathway between patients with ARHI and healthy subjects...
2016: Clinical Interventions in Aging
M Salmona, A Caporossi, P Simmonds, M-A Thélu, K Fusillier, S Mercier-Delarue, N De Castro, J LeGoff, M-L Chaix, O François, F Simon, P Morand, S Larrat, S Maylin
We report the near-full-length genome sequence of a hepatitis C virus (HCV) isolate from a man originating from Democratic Republic of Congo, the genotype of which could not be determined by the routinely used sequencing technique. The near-complete genome sequence of this variant BAK1 was obtained by the association of two next-generation sequencing technologies. Evolutionary analysis indicates that this isolate, BAK1, could be the first reported strain belonging to a new HCV-7b subtype. This new subtype has been incorrectly identified as genotype 2 by the Versant HCV Genotype 2...
November 2016: Clinical Microbiology and Infection
Daniel Couto, Roda Niebergall, Xiangxiu Liang, Christoph A Bücherl, Jan Sklenar, Alberto P Macho, Vardis Ntoukakis, Paul Derbyshire, Denise Altenbach, Dan Maclean, Silke Robatzek, Joachim Uhrig, Frank Menke, Jian-Min Zhou, Cyril Zipfel
Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI is negatively regulated is not fully understood. Here, we identify the protein phosphatase PP2C38 as a negative regulator of BIK1 activity and BIK1-mediated immunity. PP2C38 dynamically associates with BIK1, as well as with the PRRs FLS2 and EFR, but not with the co-receptor BAK1...
August 2016: PLoS Pathogens
Heqiao Zhang, Xiaoya Lin, Zhifu Han, Jizong Wang, Li-Jia Qu, Jijie Chai
In Arabidopsis, the CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides play important roles in regulating proliferation and differentiation of plant-specific stem cells. Although receptors of CLEs are reported to be leucine-rich repeat receptor kinases, the mechanisms underlying CLE-induced receptor activation remain largely unknown. Here we show that SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs) serve as co-receptors in CLE41/TDIF-PXY signaling to regulate plant vascular development. TDIF induces interaction of its receptor PXY with SERKs in vitro and in vivo...
October 10, 2016: Molecular Plant
(no author information available yet)
[This retracts the article on p. 16 in vol. 5, PMID: 24550926.].
2016: Frontiers in Plant Science
Lucía Jordá, Sara Sopeña-Torres, Viviana Escudero, Beatriz Nuñez-Corcuera, Magdalena Delgado-Cerezo, Keiko U Torii, Antonio Molina
ERECTA (ER) receptor-like kinase (RLK) regulates Arabidopsis thaliana organ growth, and inflorescence and stomatal development by interacting with the ERECTA-family genes (ERf) paralogs, ER-like 1 (ERL1) and ERL2, and the receptor-like protein (RLP) TOO MANY MOUTHS (TMM). ER also controls immune responses and resistance to pathogens such as the bacterium Pseudomonas syringae pv. tomato DC3000 (Pto) and the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM). We found that er null-mutant plants overexpressing an ER dominant-negative version lacking the cytoplasmic kinase domain (ERΔK) showed an enhanced susceptibility to PcBMM, suggesting that ERΔK associates and forms inactive complexes with additional RLKs/RLPs required for PcBMM resistance...
2016: Frontiers in Plant Science
Tran Ngoc Dang, Izumi Naka, Areerat Sa-Ngasang, Surapee Anantapreecha, Nuanjun Wichukchinda, Pathom Sawanpanyalert, Jintana Patarapotikul, Naoyuki Tsuchiya, Jun Ohashi
BACKGROUND: Dengue hemorrhagic fever (DHF) is a severe life-threatening form of dengue infection. Low platelet count is one of the characteristic clinical manifestations in patients with severe dengue. However, little is known about genetic factors in the host that cause low platelet count in patients with dengue. METHODS: A previous genome-wide association study of hematological and biochemical traits identified single nucleotide polymorphisms (SNPs) associated with low platelet count in healthy subjects...
2016: BMC Medical Genetics
Yanan Liu, Xingchuan Huang, Meng Li, Ping He, Yuelin Zhang
The Arabidopsis receptor-like kinase (RLK) BIR1 (BAK1-INTERACTING RECEPTOR-LIKE KINASE 1) functions as a negative regulator of plant immunity. Previous work showed that loss-of-function of BIR1 leads to constitutive activation of cell death and defense responses. These autoimmune phenotypes are partially dependent on another RLK, SOBIR1. In order to identify additional components involved in the BIR1-regulated plant defense signaling pathway, a suppressor screen was carried out in the bir1-1 pad4-1 mutant background...
July 12, 2016: New Phytologist
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