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Ulrich Hohmann, Julia Santiago, Joël Nicolet, Vilde Olsson, Fabio M Spiga, Ludwig A Hothorn, Melinka A Butenko, Michael Hothorn
Plant-unique membrane receptor kinases with leucine-rich repeat ectodomains (LRR-RKs) can sense small molecule, peptide, and protein ligands. Many LRR-RKs require SERK-family coreceptor kinases for high-affinity ligand binding and receptor activation. How one coreceptor can contribute to the specific binding of distinct ligands and activation of different LRR-RKs is poorly understood. Here we quantitatively analyze the contribution of SERK3 to ligand binding and activation of the brassinosteroid receptor BRI1 and the peptide hormone receptor HAESA...
March 12, 2018: Proceedings of the National Academy of Sciences of the United States of America
Ning Yuan, Shuangrong Yuan, Zhigang Li, Man Zhou, Peipei Wu, Qian Hu, Liangjiang Wang, Venugopal Mendu, Hong Luo
Protein kinases play fundamental roles in plant development and environmental stress responses. Here, we identified the STRESS INDUCED FACTOR (SIF) gene family, which encodes four leucine-rich repeat receptor-like protein kinases (LRR-RLKs) in Arabidopsis thaliana. The four genes, SIF1-4, are clustered in the genome and highly conserved, but have temporally and spatially distinct expression patterns. We employed Arabidopsis SIF knockout mutants and overexpression transgenics to examine SIF involvement during plant pathogen defense...
February 20, 2018: Plant Physiology
Zhiqiang Zhang, Liping Xu
Brassinosteroids (BRs) are plant-specific steroidal hormones, the homeostasis of which is crucial for various aspects of plant growth and development. However, to date, the BR inactivation process has not been thoroughly elucidated. In this study, we identified and characterized a novel BAHD family acyltransferase gene, BRASSINOSTEROID INACTIVATOR2 (BIA2), involved in BR inactivation. Plants overexpressing BIA2 displayed typical BR-deficient phenotypes, which were rescued by exogenous BR treatment. Real-time qRT-PCR and transcriptome analyses showed that virtually all of the BR-biosynthetic gene expression levels were increased, whereas the expression of many BR- inactivation genes was reduced in OE-BIA2 plants...
February 15, 2018: Journal of Experimental Botany
Lei Wang, Elias Einig, Marilia Almeida-Trapp, Markus Albert, Judith Fliegmann, Axel Mithöfer, Hubert Kalbacher, Georg Felix
The discovery in tomato of systemin, the first plant peptide hormone1,2 , was a fundamental change for the concept of plant hormones. Numerous other peptides have since been shown to play regulatory roles in many aspects of the plant life, including growth, development, fertilization and interactions with symbiotic organisms3-6 . Systemin, an 18 amino acid peptide derived from a larger precursor protein7 , was proposed to act as the spreading signal that triggers systemic defence responses observed in plants after wounding or attack by herbivores1,7,8 ...
February 19, 2018: Nature Plants
Rui Miao, Meng Wang, Wei Yuan, Yan Ren, Ying Li, Na Zhang, Jianhua Zhang, Herbert J Kronzucker, Weifeng Xu
Plant root responds to soil moisture gradients through hydrotropism and remodels root growth orientation chasing soil water, a process regarded as plant acclimation to a changing soil environment. Mechanisms underlying the root hydrotropic response, however, remain poorly understood. Here, hydrotropism was examined in 31 Arabidopsis ecotypes collected from different parts of the world and grown along moisture gradients in a specially designed soil-simulation system. More detailed comparative transcriptomic, ionomic profiling, and physiological analyses were carried out on three wild types, Ws (strongly hydrotropic), Col-0 (moderately hydrotropic), and C24 (weakly hydrotropic), and also in mutant lines showing disruptions in the root hydrotropic response...
February 8, 2018: Plant Physiology
Jinggeng Zhou, Derui Liu, Ping Wang, Xiyu Ma, Wenwei Lin, Sixue Chen, Kiril Mishev, Dongping Lu, Rahul Kumar, Isabelle Vanhoutte, Xiangzong Meng, Ping He, Eugenia Russinova, Libo Shan
Plants largely rely on plasma membrane (PM)-resident receptor-like kinases (RLKs) to sense extracellular and intracellular stimuli and coordinate cell differentiation, growth, and immunity. Several RLKs have been shown to undergo internalization through the endocytic pathway with a poorly understood mechanism. Here, we show that endocytosis and protein abundance of the Arabidopsis brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), are regulated by plant U-box (PUB) E3 ubiquitin ligase PUB12- and PUB13-mediated ubiquitination...
February 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Tong Liang, Shenglin Mei, Chen Shi, Yu Yang, Yao Peng, Libang Ma, Fei Wang, Xu Li, Xi Huang, Yanhai Yin, Hongtao Liu
UV-B light (UV-B radiation) is known to inhibit plant growth, but the mechanism is not well understood. UVR8 (UV RESISTANCE LOCUS 8) is a UV-B light photoreceptor that mediates UV-B light responses in plants. We report here that UV-B inhibits plant growth by repressing plant steroid hormone brassinosteroid (BR)-promoted plant growth. UVR8 physically interacts with the functional dephosphorylated BES1 (BRI1-EMS-SUPPRESSOR1) and BIM1 (BES1-INTERACTING MYC-LIKE 1) transcription factors that mediate BR-regulated gene expression and plant growth to inhibit their activities...
January 26, 2018: Developmental Cell
Masato Saito, Yuki Kondo, Hiroo Fukuda
Vascular development is a good model for studying cell differentiation in plants. Two conductive tissues, the xylem and phloem, are derived from common stem cells known as procambial/cambial cells. Glycogen synthase kinase 3 proteins (GSK3s) play crucial roles in maintaining procambial/cambial cells by suppressing their differentiation into xylem or phloem cells. We previously designed an in vitro culture system for analyzing vascular cell differentiation named VISUAL (Vascular cell Induction culture System Using Arabidopsis Leaves)...
January 27, 2018: Plant & Cell Physiology
Eun-Seok Oh, Yeon Lee, Won Byoung Chae, Jana Jeevan Rameneni, Yong-Soon Park, Yong Pyo Lim, Man-Ho Oh
Protein post-translational modification by phosphorylation is essential for the activity and stability of proteins in higher plants and underlies their responses to diverse stimuli. There are more than 300 leucine-rich repeat receptor-like kinases (LRR-RLKs), a major group of receptor-like kinases (RLKs) that plays an important role in growth, development, and biotic stress responses in higher plants. To analyze auto- and transphosphorylation patterns and kinase activities in vitro, 43 full-length complementary DNA (cDNA) sequences were cloned from genes encoding LRR-RLKs...
January 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hong Hanh Nguyen, Myoung Hui Lee, Kyungyoung Song, Gyeongik Ahn, Jihyeong Lee, Inhwan Hwang
Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacuole for degradation or recycled back to the plasma membrane. How this occurs at the molecular level remains largely elusive. Here we provide evidence that the ENTH domain-containing protein AtECA4 plays a crucial role in recycling cargoes from the TGN/EE to the plasma membrane...
January 6, 2018: Molecular Plant
Ji Hyea Lee, Jeongyeo Lee, HyeRan Kim, Won Byoung Chae, Sun-Ju Kim, Yong Pyo Lim, Man-Ho Oh
Plants must constantly adjust their growth and defense responses to deal with the wide variety of stresses they encounter in their environment. Among phytohormones, brassinosteroids (BRs) are an important group of plant steroid hormones involved in numerous aspects of the plant lifecycle including growth, development, and responses to various stresses including insects. Here, we show that BRs regulate glucosinolate (GS) biosynthesis and function in insect herbivory. Preference tests and larval feeding experiments using the generalist herbivore, diamondback moth (Plutella xylostella), revealed that the larvae of this moth prefer to feed on Arabidopsis thaliana brassinosteroid insensitive 1 (bri1-5) plants than on wild-type Ws-2 or BRI1-Flag (bri1-5 background) transgenic plants, which leads an increase in larval weight...
January 5, 2018: Physiologia Plantarum
Qinsong Liu, Thomas Vain, Corrado Viotti, Siamsa M Doyle, Danuše Tarkowská, Ondřej Novák, Cyril Zipfel, Folke Sitbon, Stéphanie Robert, Daniel Hofius
Brassinosteroid (BR) hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1) together with co-receptors such as BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1). BRI1 abundance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remain elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 (LAZ1) and LAZ1 HOMOLOG1 (LAZ1H1), causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling...
December 27, 2017: Molecular Plant
Fidel Lozano-Elena, Ainoa Planas-Riverola, Josep Vilarrasa-Blasi, Rebecca Schwab, Ana I Caño-Delgado
Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) acts as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of the QC have a very low mitotic activity, plant hormones such as brassinosteroids (BR) can promote QC divisions. Here, we used a tissue-specific strategy to investigate the spatial signaling requirements of BR-mediated QC divisions...
December 14, 2017: Journal of Cell Science
Hyunggon Mang, Baomin Feng, Zhangjian Hu, Aurélien Boisson-Dernier, Christina M Franck, Xiangzong Meng, Yanyan Huang, Jinggeng Zhou, Guangyuan Xu, Taotao Wang, Libo Shan, Ping He
Plants have evolved two tiers of immune receptors to detect infections: cell surface-resident pattern recognition receptors (PRRs) that sense microbial signatures and intracellular nucleotide binding domain leucine-rich repeat (NLR) proteins that recognize pathogen effectors. How PRRs and NLRs interconnect and activate the specific and overlapping plant immune responses remains elusive. A genetic screen for components controlling plant immunity identified ANXUR1 (ANX1), a malectin-like domain-containing receptor-like kinase, together with its homolog ANX2, as important negative regulators of both PRR- and NLR-mediated immunity in Arabidopsis thaliana ANX1 constitutively associates with the bacterial flagellin receptor FLAGELLIN-SENSING2 (FLS2) and its coreceptor BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1)...
December 2017: Plant Cell
Keini Dressano, Paulo H O Ceciliato, Aparecida L Silva, Juan Carlos Guerrero-Abad, Tábata Bergonci, Fausto Andrés Ortiz-Morea, Marco Bürger, Marcio C Silva-Filho, Daniel S Moura
The rapid alkalinization factor (RALF) peptide negatively regulates cell expansion, and an antagonistic relationship has been demonstrated between AtRALF1, a root-specific RALF isoform in Arabidopsis, and brassinosteroids (BRs). An evaluation of the response of BR signaling mutants to AtRALF1 revealed that BRI1-associated receptor kinase1 (bak1) mutants are insensitive to AtRALF1 root growth inhibition activity. BAK1 was essential for the induction of AtRALF1-responsive genes but showed no effect on the mobilization of Ca2+ and alkalinization responses...
October 2017: PLoS Genetics
Shuhua Huang, Shuming Nie, Shufen Wang, Jianwei Liu, Yanfeng Zhang, Xiaofeng Wang
Bri1-associated kinase 1 (BAK1)-interacting receptor-like kinase (BIR) proteins have been shown to play important roles in regulating growth and development, pathogen associated molecular pattern (PAMP)-triggered immunity (PTI) responses, and cell death in the model plant, Arabidopsis thaliana. We identified four BIR family members in tomato (Solanum lycopersicum), including SlBIR3, an ortholog of AtBIR3 from A. thaliana. SlBIR3 is predicted to encode a membrane localized non-arginine-aspartate (non-RD) kinase that, based on protein sequence, does not have autophosphorylation activity but that can be phosphorylated in vivo...
September 13, 2017: International Journal of Molecular Sciences
Meihua Fan, Xue Sun, Nianjun Xu, Zhi Liao, Yahe Li, Jianxin Wang, Yingping Fan, Dalian Cui, Peng Li, Zengliang Miao
To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified...
September 8, 2017: Scientific Reports
Ming Zhang, Jinfeng Zhao, Long Li, Yanan Gao, Linlin Zhao, Suyash Bhimgonda Patil, Jingjing Fang, Wenhui Zhang, Yuhong Yang, Ming Li, Xueyong Li
The Arabidopsis U-box E3 ubiquitin ligases play an important role in the ubiquitin/26S proteasome-mediated protein degradation pathway. Recently, PUB30 has been reported to participate in the salt stress response during seed germination stage in abscisic acid (ABA)-independent manner, but the molecular mechanism remains to be elucidated. Here, we displayed that the pub30 mutant was more tolerant to salt stress during seed germination, whereas the mutant of its closest homologue PUB31 showed mild sensitivity to salt stress...
September 2, 2017: Plant, Cell & Environment
Julia Imkampe, Thierry Halter, Shuhua Huang, Sarina Schulze, Sara Mazzotta, Nikola Schmidt, Raffaele Manstretta, Sandra Postel, Michael Wierzba, Yong Yang, Walter M A M van Dongen, Mark Stahl, Cyril Zipfel, Michael B Goshe, Steven Clouse, Sacco C de Vries, Frans Tax, Xiaofeng Wang, Birgit Kemmerling
BAK1 is a coreceptor and positive regulator of multiple ligand binding leucine-rich repeat receptor kinases (LRR-RKs) and is involved in brassinosteroid (BR)-dependent growth and development, innate immunity, and cell death control. The BAK1-interacting LRR-RKs BIR2 and BIR3 were previously identified by proteomics analyses of in vivo BAK1 complexes. Here, we show that BAK1-related pathways such as innate immunity and cell death control are affected by BIR3 in Arabidopsis thaliana BIR3 also has a strong negative impact on BR signaling...
September 2017: Plant Cell
Xiaofei Cheng, Xiaoping Gou, Hongju Yin, Kirankumar S Mysore, Jia Li, Jiangqi Wen
Brassinosteroids are phytohormones involved in plant development and physiological processes. Brassinosteroids Insensitive 1 (BRI1) is required for BR perception and initiation of subsequent signal transduction in Arabidopsis. In this study, the orthologue of BRI1 in the model legume species Medicago truncatula, MtBRI1, was identified and characterised. Three allelic Tnt1 insertion mutants, mtbri1-1, mtbri1-2, and mtbri1-3, were obtained from the M. truncatula Tnt1 insertion population. mtbri1 mutants displayed characteristic bri1 mutant phenotypes: extreme dwarfness, dark green curled leaves, short primary roots, less lateral roots, and insensitive to exogenous brassinolide (BL)...
August 24, 2017: Scientific Reports
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