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https://www.readbyqxmd.com/read/28413947/novel-signaling-interface-constituted-with-membrane-receptor-like-kinases-emerged-from-the-study-of-interaction-and-transphosphorylation-of-bri1-and-bak1
#1
Cheng Li, Shan Zhang, Xiaofeng Wang
BRI1 and BAK1 are Receptor-Like Kinases (RLKs), one of the largest gene families in plants participating in various cell signal transduction from cell surface to cytoplasm with oligomerization and phosphorylation to regulate plant growth, development, immunity, and environmental responses. Based on the recent investigations on the BRI1 and BAK1 and other RLKs involving in the receptor complex formation, trans phosphorylation, phosphorylation sites identification, downstream substrates identification, and so on, it is recovered that the receptors oligomerization and phosphorylation integrate multiple distinct signaling to realize signaling modulation, divergence, convergence, and specificity...
April 14, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28407091/brassinosteroid-involvement-in-arabidopsis-thaliana-stomatal-opening
#2
Shin-Ichiro Inoue, Nozomi Iwashita, Yohei Takahashi, Eiji Gotoh, Eiji Okuma, Maki Hayashi, Ryohei Tabata, Atsushi Takemiya, Yoshiyuki Murata, Michio Doi, Toshinori Kinoshita, Ken-Ichiro Shimazaki
Stomata within the plant epidermis regulate CO2 uptake for photosynthesis and water loss through transpiration. Stomatal opening in Arabidopsis thaliana is determined by various factors, including blue light as a signal and multiple phytohormones. Plasma membrane transporters, including H+-ATPase, K+ channels, and anion channels in guard cells mediate these processes, and the activities and expression levels of these components determine stomatal aperture. However, the regulatory mechanisms involved in these processes are not fully understood...
April 12, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28399399/sinat-e3-ligases-control-the-light-mediated-stability-of-the-brassinosteroid-activated-transcription-factor-bes1-in-arabidopsis
#3
Mengran Yang, Chengxiang Li, Zhenying Cai, Yinmeng Hu, Trevor Nolan, Feifei Yu, Yanhai Yin, Qi Xie, Guiliang Tang, Xuelu Wang
The plant hormones brassinosteroids (BRs) participate in light-mediated regulation of plant growth, although the underlying mechanisms are far from being fully understood. In addition, the function of the core transcription factor in the BR signaling pathway, BRI1-EMS-SUPPRESSOR 1 (BES1), largely depends on its phosphorylation status and its protein stability, but the regulation of BES1 is not well understood. Here, we report that SINA of Arabidopsis thaliana (SINATs) specifically interact with dephosphorylated BES1 and mediate its ubiquitination and degradation...
April 10, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28369122/molecular-actions-of-two-synthetic-brassinosteroids-iso-carbabl-and-6-deoxobl-which-cause-altered-physiological-activities-between-arabidopsis-and-rice
#4
Ayako Nakamura, Naoya Tochio, Shozo Fujioka, Shinsaku Ito, Takanori Kigawa, Yukihisa Shimada, Makoto Matsuoka, Shigeo Yoshida, Toshinori Kinoshita, Tadao Asami, Hideharu Seto, Takeshi Nakano
Brassinosteroid (BR) is an important plant hormone that is perceived by the BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor. BRI1 is conserved among dicot and monocot species; however, the molecular mechanism underlying BR perception in monocots is not fully understood. We synthesised two BRs, iso-carbabrassinolide (iso-carbaBL) and 6-deoxoBL, which have different BR activities in Arabidopsis thaliana (Arabidopsis) and rice. Our bioassay indicated that iso-carbaBL has relatively strong BR activity in Arabidopsis, but is inactive in rice and competitively inhibits BR activity...
2017: PloS One
https://www.readbyqxmd.com/read/28331193/transcription-factors-of-schizophyllum-commune-involved-in-mushroom-formation-and-modulation-of-vegetative-growth
#5
Jordi F Pelkmans, Mohini B Patil, Thies Gehrmann, Marcel J T Reinders, Han A B Wösten, Luis G Lugones
Mushrooms are the most conspicuous fungal structures. Transcription factors (TFs) Bri1 and Hom1 of the model fungus Schizophyllum commune are involved in late stages of mushroom development, while Wc-2, Hom2, and Fst4 function early in development. Here, it is shown that Bri1 and Hom1 also stimulate vegetative growth, while biomass formation is repressed by Wc-2, Hom2, and Fst4. The Δbri1Δbri1 and the Δhom1Δhom1 strains formed up to 0.6 fold less biomass when compared to wild-type, while Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains formed up to 2...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28320734/topless-mediates-brassinosteroid-control-of-shoot-boundaries-and-root-meristem-development-in-arabidopsis-thaliana
#6
Ana Espinosa-Ruiz, Cristina Martínez, Miguel de Lucas, Norma Fàbregas, Nadja Bosch, Ana I Caño-Delgado, Salomé Prat
The transcription factor bri1-EMS-SUPRESSOR1 (BES1) is a master regulator of brassinosteroid (BR)-regulated gene expression. BES1 together with BRASSINAZOLE-RESISTANT1 (BZR1) drive activated or repressed expression of several genes, and have a prominent role in negative regulation of BR synthesis. Here, we report that BES1 interaction with TOPLESS (TPL), via its ERF-associated amphiphilic repression EAR motif, is essential for BES1-mediated control of organ boundary formation in the SAM and the regulation of the quiescent center (QC) cell division in roots...
March 20, 2017: Development
https://www.readbyqxmd.com/read/28262094/plant-immune-and-growth-receptors-share-common-signalling-components-but-localise-to-distinct-plasma-membrane-nanodomains
#7
Christoph A Bücherl, Iris K Jarsch, Christian Schudoma, Cécile Segonzac, Malick Mbengue, Silke Robatzek, Daniel MacLean, Thomas Ott, Cyril Zipfel
Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters...
March 6, 2017: ELife
https://www.readbyqxmd.com/read/28153720/overexpression-of-bak1-causes-salicylic-acid-accumulation-and-deregulation-of-cell-death-control-genes
#8
Sun Young Kim, Yun Shang, Se-Hwan Joo, Seong-Ki Kim, Kyoung Hee Nam
Since the BRI1-Associated Receptor Kinase 1 (BAK1) was firstly identified as a co-receptor of BRI1 that mediates brassinosteroids (BR) signaling, the functional roles of BAK1, as a versatile co-receptor for various ligand-binding leucine-rich repeat (LRR)-containing receptor-like kinase (RLKs), are being extended to involvement with plant immunity, cell death, stomatal development and ABA signaling in plants. During more than a decade of research on the BAK1, it has been known that transgenic Arabidopsis plants overexpressing BAK1 tagged with various reporters do not fully represent its natural functions...
January 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28124254/probing-activation-and-deactivation-of-the-brassinosteroid-insensitive1-receptor-kinase-by-immunoprecipitation
#9
Sara Martins, Grégory Vert, Yvon Jaillais
Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124252/identification-of-brassinosteroid-signaling-complexes-by-coimmunoprecipitation-and-mass-spectrometry
#10
Walter van Dongen, Luc van Heerde, Sjef Boeren, Sacco C de Vries
A combination of coimmunoprecipitation (coIP) of tagged proteins followed by protein identification and quantitation using Liquid Chromatography Mass Spectrometry/Mass Spectrometry (LCMS/MS) has proven to be a reliable method to qualitatively characterize membrane-bound receptor complexes from plants. Success depends on a range of parameters, such as abundance and stability of the complex and functionality of the tagged receptors, efficiency of the protein complex isolation procedure, MS equipment, and analysis software in use...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124251/analysis-of-in-vitro-dna-interactions-of-brassinosteroid-controlled-transcription-factors-using-electrophoretic-mobility-shift-assay
#11
Simon J Unterholzner, Wilfried Rozhon, Brigitte Poppenberger
Most signaling cascades ultimately lead to changes in gene expression by modulating the activity of transcription factors (TFs). The electrophoretic mobility shift assay (EMSA) is a simple but powerful in vitro method for investigation of specific protein-DNA interactions. It makes use of the fact that protein-DNA complexes have a lower electrophoretic mobility in gels than free DNA has. The application of labeled probes in combination with unlabeled competitors allows investigation of DNA-binding specificity and identification of binding motifs with single base-pair resolution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124250/quantitative-microscopic-analysis-of-plasma-membrane-receptor-dynamics-in-living-plant-cells
#12
Yu Luo, Eugenia Russinova
Plasma membrane-localized receptors are essential for cellular communication and signal transduction. In Arabidopsis thaliana, BRASSINOSTEROID INSENSITIVE1 (BRI1) is one of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endomembrane trafficking. Advances in fluorescence labeling and confocal microscopy techniques enabled us to gain a better understanding of plasma membrane receptor dynamics in living cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124246/a-technical-framework-for-studying-the-signaling-nexus-of-brassinosteroids-and-immunity
#13
Rosa Lozano-Durán, Youssef Belkhadir
Pathway cross-communication cannot be simply tackled by studying isolated signaling systems. Yet understanding how signal transduction pathways attenuate or reinforce each other in vivo is a challenging task. In plants, biosynthesis and signaling of brassinosteroids (BRs) finely regulate growth and defense programs through a complex array of mechanistic and physiological interactions. Conversely, induction of defenses also impacts on the BR biosynthesis at the transcriptional level. In this chapter, we present an experimental framework to study the physiological connection between BR-controlled growth and defenses...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28114413/visualization-of-bri1-and-serk3-bak1-nanoclusters-in-arabidopsis-roots
#14
Stefan J Hutten, Danny S Hamers, Marije Aan den Toorn, Wilma van Esse, Antsje Nolles, Christoph A Bücherl, Sacco C de Vries, Johannes Hohlbein, Jan Willem Borst
Brassinosteroids (BRs) are plant hormones that are perceived at the plasma membrane (PM) by the ligand binding receptor BRASSINOSTEROID-INSENSITIVE1 (BRI1) and the co-receptor SOMATIC EMBRYOGENESIS RECEPTOR LIKE KINASE 3/BRI1 ASSOCIATED KINASE 1 (SERK3/BAK1). To visualize BRI1-GFP and SERK3/BAK1-mCherry in the plane of the PM, variable-angle epifluorescence microscopy (VAEM) was employed, which allows selective illumination of a thin surface layer. VAEM revealed an inhomogeneous distribution of BRI1-GFP and SERK3/BAK1-mCherry at the PM, which we attribute to the presence of distinct nanoclusters...
2017: PloS One
https://www.readbyqxmd.com/read/28096215/brassinosteroid-signaling-directs-formative-cell-divisions-and-protophloem-differentiation-in-arabidopsis-root-meristems
#15
Yeon Hee Kang, Alice Breda, Christian S Hardtke
Brassinosteroids (BRs) trigger an intracellular signaling cascade through its receptors BR INSENSITIVE 1 (BRI1), BRI1-LIKE 1 (BRL1) and BRL3. Recent studies suggest that BR-independent inputs related to vascular differentiation, for instance root protophloem development, modulate downstream BR signaling components. Here, we report that protophloem sieve element differentiation is indeed impaired in bri1 brl1 brl3 mutants, although this effect might not be mediated by canonical downstream BR signaling components...
January 15, 2017: Development
https://www.readbyqxmd.com/read/28069895/brassinosteroid-induced-transcriptional-repression-and-dephosphorylation-dependent-protein-degradation-negatively-regulate-bin2-interacting-aif2-a-br-signaling-negative-regulator-bhlh-transcription-factor
#16
Yoon Kim, Ji-Hye Song, Seon-U Park, You-Seung Jeong, Soo-Hwan Kim
Brassinosteroids (BRs) are plant polyhydroxy-steroids that play important roles in plant growth and development via extensive signal integration through direct interactions between regulatory components of different signaling pathways. Recent studies have shown that diverse helix-loop-helix/basic helix-loop-helix (HLH/bHLH) family proteins are actively involved in control of BR signaling pathways and interact with other signaling pathways. In this study, we show that ATBS1-INTERACTING FACTOR 2 (AIF2), a nuclear-localized atypical bHLH transcription factor, specifically interacts with BRASSINOSTEROID-INSENSITIVE 2 (BIN2) among other BR signaling molecules...
February 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28061864/identification-of-brassinosteroid-genes-in-brachypodium-distachyon
#17
Claudia Corvalán, Sunghwa Choe
BACKGROUND: Brassinosteroids (BRs) are steroidal phytohormones that are involved in diverse physiological processes and affect many important traits, such as plant stature, stress tolerance, leaf angle, fertility, and grain filling. BR signaling and biosynthetic pathways have been studied in various plants, such as the model dicot Arabidopsis thaliana; however, relatively little is known about these pathways in monocots. RESULTS: To characterize BR-related processes in the model grass Brachypodium distachyon, we studied the response of these plants to the specific BR biosynthesis inhibitor, propiconazole (Pcz)...
January 6, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28035023/related-to-abi3-vp1-like-1-ravl1-regulates-brassinosteroid-mediated-activation-of-amt1-2-in-rice-oryza-sativa
#18
Yuan Hu Xuan, Feng Ying Duan, Byoung Il Je, Chul Min Kim, Tian Ya Li, Jing Miao Liu, Soon Ju Park, Jun Hyeon Cho, Tae Ho Kim, Nicolaus von Wiren, Chang-Deok Han
The promotive effects of brassinosteroids (BRs) on plant growth and development have been widely investigated; however, it is not known whether BRs directly affect nutrient uptake. Here, we explored the possibility of a direct relationship between BRs and ammonium uptake via AMT1-type genes in rice (Oryza sativa). BR treatment increased the expression of AMT1;1 and AMT1;2, whereas in the mutant d61-1, which is defective in the BR-receptor gene BRI1, BR-dependent expression of these genes was suppressed. We then employed Related to ABI3/VP1-Like 1 (RAVL1), which is involved in BR homeostasis, to investigate BR-mediated AMT1 expression and its effect on NH4+ uptake in rice roots...
January 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27995670/the-tomato-i-gene-for-fusarium-wilt-resistance-encodes-an-atypical-leucine-rich-repeat-receptor-like-protein-whose-function-is-nevertheless-dependent-on-sobir1-and-serk3-bak1
#19
Ann-Maree Catanzariti, Huong T T Do, Pierrick Bru, Mara de Sain, Louise F Thatcher, Martijn Rep, David A Jones
We have identified the tomato I gene for resistance to the Fusarium wilt fungus Fusarium oxysporum f. sp. lycopersici (Fol) and show that it encodes a membrane-anchored leucine-rich repeat receptor-like protein (LRR-RLP). Unlike most other LRR-RLP genes involved in plant defence, the I gene is not a member of a gene cluster and contains introns in its coding sequence. The I gene encodes a loopout domain larger than those in most other LRR-RLPs, with a distinct composition rich in serine and threonine residues...
December 20, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27988365/bki1-regulates-plant-architecture-through-coordinated-inhibition-of-the-brassinosteroid-and-erecta-signaling-pathways-in-arabidopsis
#20
Dongxu Wang, Cangjing Yang, Haijiao Wang, Zhihua Wu, Jianjun Jiang, Jingjing Liu, Zhuona He, Fang Chang, Hong Ma, Xuelu Wang
Hundreds of leucine-rich repeat receptor-like kinases (LRR-RLKs) play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inactive status are essential but rarely studied. BKI1 is the only reported inhibitor of receptor kinases in Arabidopsis, which negatively regulates BRI1 in the brassinosteroid pathway. In this study, we found that BKI1 can also interact with another important LRR-RLK, ERECTA (ER). Phenotypic analysis showed that BKI1 and ER together regulate plant architecture, including pedicel orientation, which is a newly reported phenotype in the BR- and ER-mediated developmental processes...
December 14, 2016: Molecular Plant
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