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https://www.readbyqxmd.com/read/28578021/transcriptome-profiling-of-the-floral-buds-and-discovery-of-genes-related-to-sex-differentiation-in-the-dioecious-cucurbit-coccinia-grandis-l-voigt
#1
Jatindra Nath Mohanty, Sanghamitra Nayak, Sumita Jha, Raj Kumar Joshi
Dioecious species offer an inclusive structure to study the molecular basis of sexual dimorphism in angiosperms. Despite having a small genome and heteromorphic sex chromosomes, Coccinia grandis is a highly neglected dioecious species with little information available on its physical state, genetic orientation and key sex-defining elements. In the present study, we performed RNA-Seq and DGE analysis of male (MB) and female (FB) buds in C. grandis to gain insights into the molecular basis of sex determination in this plant...
May 31, 2017: Gene
https://www.readbyqxmd.com/read/28559283/molecular-dynamics-simulations-reveal-the-conformational-dynamics-of-arabidopsis-thaliana-bri1-and-bak1-receptor-like-kinases
#2
Alexander S Moffett, Kyle W Bender, Steven C Huber, Diwakar Shukla
The structural motifs responsible for activation and regulation of eukaryotic protein kinases in animals have been studied extensively in recent years, and a coherent picture of their activation mechanisms has begun to emerge. On the other hand, non-animal eukaryotic protein kinases are not as well understood from a structural perspective, representing a large knowledge gap. To this end, we investigated the conformational dynamics of two key Arabidopsis thaliana receptor-like kinases, brassi- nosteroid insensitive 1 (BRI1) and BRI1-associated kinase 1 (BAK1), through extensive molecular dynamics (MD) simulations of their fully phosphorylated kinase domains...
May 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28545052/brl3-and-atrgs1-cooperate-to-fine-tune-growth-inhibition-and-ros-activation
#3
Meral Tunc-Ozdemir, Alan M Jones
Plasma membrane-localized leucine-rich repeat receptor-like kinases directly activates G protein complex via interaction with seven transmembrane domain Regulator of G-protein Signaling 1 (AtRGS1) protein. Brassinosteroid insensitive 1 (BRI1) LIKE3 (BRL3) phosphorylates AtRGS1 in vitro. FRET analysis showed that BRL3 and AtRGS1 interaction dynamics change in response to glucose and flg22. Both BRL3 and AtRGS1 function in glucose sensing and brl3 and rgs1-2 single mutants are hyposensitive to high glucose as well as the brl3/rgs1 double mutant...
2017: PloS One
https://www.readbyqxmd.com/read/28540949/structure-based-in-silico-identification-of-potentially-non-steroidal-brassinosteroids-mimics
#4
Beilei Lei, Ningjuan Heng, Xiaoxue Dang, Jiyuan Liu, Xiaojun Yao, Cunli Zhang
Brassinosteroids (BRs) are a class of plant steroid hormones that play indispensable roles in cell elongation, division and plant development. To date, the numerous synthesis of BRs analogs and structure-activity relationship investigations have clearly revealed the key substituent groups relevant to the steroidal activity of BRs. However, due to the limited chemical space studied, the efforts for alternative non-steroidal compounds have produced no remarkable results. To identify potentially non-steroidal BR mimics in this study, vital interacting pharmacophore features were extracted starting from several complex structures of BRs that bound with the receptor Brassinosteroid-Insentive 1 (BRI1) and co-receptor BRI1-associated kinase 1 (BAK1), which were characterized and merged into one comprehensive pharmacophore model...
May 25, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28497809/receptor-like-protein-elt1-promotes-brassinosteroid-signaling-through-interacting-with-and-suppressing-the-endocytosis-mediated-degradation-of-receptor-bri1
#5
Bao-Jun Yang, Wen-Hui Lin, Fang-Fang Fu, Zhi-Hong Xu, Hong-Wei Xue
No abstract text is available yet for this article.
May 12, 2017: Cell Research
https://www.readbyqxmd.com/read/28482142/the-brassinosteroid-receptor-bri1-can-generate-cgmp-enabling-cgmp-dependent-downstream-signaling
#6
Janet I Wheeler, Aloysius Wong, Claudius Marondedze, Arnoud J Groen, Lusisizwe Kwezi, Lubna Freihat, Jignesh Vyas, Misjudeen A Raji, Helen R Irving, Chris Gehring
The brassinosteroid receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) is a member of the leucine rich repeat receptor like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per μg protein with a preference for magnesium over manganese as a co-factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation...
May 8, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28461403/scanning-for-new-bri1-receptor-mutations-via-tilling-analysis
#7
Chao Sun, Kan Yan, Jian-Ting Han, Liang Tao, Minghui Lv, Tao Shi, Yong-Xing He, Michael Wierzba, Frans Tax, Jia Li
Identification and characterization of a mutational spectrum for a specific protein can help to elucidate its detailed cellular functions. BRI1, a multidomain transmembrane receptor-like kinase, is a major receptor of brassinosteroids in Arabidopsis. Within the last two decades, over 20 different bri1 mutant alleles have been identified, which helped to determine the significance of each domain within BRI1. To further understand molecular mechanisms of BRI1, we tried to identify additional alleles via TILLING...
May 1, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28458047/a-look-at-plant-immunity-through-the-window-of-the-multitasking-coreceptor-bak1
#8
REVIEW
Shigetaka Yasuda, Kentaro Okada, Yusuke Saijo
Recognition of microbe- and danger-associated molecular patterns (MAMPs and DAMPs, respectively) by pattern recognition receptors (PRRs) is central to innate immunity in both plants and animals. The plant PRRs described to date are all cell surface-localized receptors. According to their ligand-binding ectodomains, each PRR engages a specific coreceptor or adaptor kinase in its signaling complexes to regulate defense signaling. With a focus on the coreceptor RLK BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) and related SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs), here we review the increasing inventory of BAK1 partners and their functions in plant immunity...
April 27, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28438793/brassinosteroid-biosynthesis-is-modulated-via-a-transcription-factor-cascade-of-cog1-pif4-and-pif5
#9
Zhuoyun Wei, Tong Yuan, Danuše Tarkowská, Jeongsik Kim, Hong Gil Nam, Ondřej Novák, Kai He, Xiaoping Gou, Jia Li
Brassinosteroids (BRs) are essential phytohormones regulating various developmental and physiological processes during normal growth and development. cog1-3D (cogwheel1-3D) was identified as an activation-tagged genetic modifier of bri1-5, an intermediate BR receptor mutant in Arabidopsis (Arabidopsis thaliana). COG1 encodes a Dof-type transcription factor found previously to act as a negative regulator of the phytochrome signaling pathway. cog1-3D single mutants show an elongated hypocotyl phenotype under light conditions...
June 2017: Plant Physiology
https://www.readbyqxmd.com/read/28413947/novel-signaling-interface-constituted-with-membrane-receptor-like-kinases-emerged-from-the-study-of-interaction-and-transphosphorylation-of-bri1-and-bak1
#10
Cheng Li, Shan Zhang, Xiaofeng Wang
BRI1 and BAK1 are Receptor-Like Kinases (RLKs), one of the largest gene families in plants participating in various cell signal transduction from cell surface to cytoplasm with oligomerization and phosphorylation to regulate plant growth, development, immunity, and environmental responses. Based on the recent investigations on the BRI1 and BAK1 and other RLKs involving in the receptor complex formation, trans phosphorylation, phosphorylation sites identification, downstream substrates identification, and so on, it is recovered that the receptors oligomerization and phosphorylation integrate multiple distinct signaling to realize signaling modulation, divergence, convergence, and specificity...
April 14, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28407091/brassinosteroid-involvement-in-arabidopsis-thaliana-stomatal-opening
#11
Shin-Ichiro Inoue, Nozomi Iwashita, Yohei Takahashi, Eiji Gotoh, Eiji Okuma, Maki Hayashi, Ryohei Tabata, Atsushi Takemiya, Yoshiyuki Murata, Michio Doi, Toshinori Kinoshita, Ken-Ichiro Shimazaki
Stomata within the plant epidermis regulate CO2 uptake for photosynthesis and water loss through transpiration. Stomatal opening in Arabidopsis thaliana is determined by various factors, including blue light as a signal and multiple phytohormones. Plasma membrane transporters, including H+-ATPase, K+ channels and anion channels in guard cells, mediate these processes, and the activities and expression levels of these components determine stomatal aperture. However, the regulatory mechanisms involved in these processes are not fully understood...
June 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28399399/sinat-e3-ligases-control-the-light-mediated-stability-of-the-brassinosteroid-activated-transcription-factor-bes1-in-arabidopsis
#12
Mengran Yang, Chengxiang Li, Zhenying Cai, Yinmeng Hu, Trevor Nolan, Feifei Yu, Yanhai Yin, Qi Xie, Guiliang Tang, Xuelu Wang
The plant hormones brassinosteroids (BRs) participate in light-mediated regulation of plant growth, although the underlying mechanisms are far from being fully understood. In addition, the function of the core transcription factor in the BR signaling pathway, BRI1-EMS-SUPPRESSOR 1 (BES1), largely depends on its phosphorylation status and its protein stability, but the regulation of BES1 is not well understood. Here, we report that SINA of Arabidopsis thaliana (SINATs) specifically interact with dephosphorylated BES1 and mediate its ubiquitination and degradation...
April 10, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28369122/molecular-actions-of-two-synthetic-brassinosteroids-iso-carbabl-and-6-deoxobl-which-cause-altered-physiological-activities-between-arabidopsis-and-rice
#13
Ayako Nakamura, Naoya Tochio, Shozo Fujioka, Shinsaku Ito, Takanori Kigawa, Yukihisa Shimada, Makoto Matsuoka, Shigeo Yoshida, Toshinori Kinoshita, Tadao Asami, Hideharu Seto, Takeshi Nakano
Brassinosteroid (BR) is an important plant hormone that is perceived by the BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor. BRI1 is conserved among dicot and monocot species; however, the molecular mechanism underlying BR perception in monocots is not fully understood. We synthesised two BRs, iso-carbabrassinolide (iso-carbaBL) and 6-deoxoBL, which have different BR activities in Arabidopsis thaliana (Arabidopsis) and rice. Our bioassay indicated that iso-carbaBL has relatively strong BR activity in Arabidopsis, but is inactive in rice and competitively inhibits BR activity...
2017: PloS One
https://www.readbyqxmd.com/read/28331193/transcription-factors-of-schizophyllum-commune-involved-in-mushroom-formation-and-modulation-of-vegetative-growth
#14
Jordi F Pelkmans, Mohini B Patil, Thies Gehrmann, Marcel J T Reinders, Han A B Wösten, Luis G Lugones
Mushrooms are the most conspicuous fungal structures. Transcription factors (TFs) Bri1 and Hom1 of the model fungus Schizophyllum commune are involved in late stages of mushroom development, while Wc-2, Hom2, and Fst4 function early in development. Here, it is shown that Bri1 and Hom1 also stimulate vegetative growth, while biomass formation is repressed by Wc-2, Hom2, and Fst4. The Δbri1Δbri1 and the Δhom1Δhom1 strains formed up to 0.6 fold less biomass when compared to wild-type, while Δwc-2Δwc-2, Δhom2Δhom2, and Δfst4Δfst4 strains formed up to 2...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28320734/topless-mediates-brassinosteroid-control-of-shoot-boundaries-and-root-meristem-development-in-arabidopsis-thaliana
#15
Ana Espinosa-Ruiz, Cristina Martínez, Miguel de Lucas, Norma Fàbregas, Nadja Bosch, Ana I Caño-Delgado, Salomé Prat
The transcription factor bri1-EMS-SUPRESSOR1 (BES1) is a master regulator of brassinosteroid (BR)-regulated gene expression. BES1 together with BRASSINAZOLE-RESISTANT1 (BZR1) drive activated or repressed expression of several genes, and have a prominent role in negative regulation of BR synthesis. Here, we report that BES1 interaction with TOPLESS (TPL), via its ERF-associated amphiphilic repression EAR motif, is essential for BES1-mediated control of organ boundary formation in the SAM and the regulation of the quiescent center (QC) cell division in roots...
March 20, 2017: Development
https://www.readbyqxmd.com/read/28262094/plant-immune-and-growth-receptors-share-common-signalling-components-but-localise-to-distinct-plasma-membrane-nanodomains
#16
Christoph A Bücherl, Iris K Jarsch, Christian Schudoma, Cécile Segonzac, Malick Mbengue, Silke Robatzek, Daniel MacLean, Thomas Ott, Cyril Zipfel
Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters...
March 6, 2017: ELife
https://www.readbyqxmd.com/read/28153720/overexpression-of-bak1-causes-salicylic-acid-accumulation-and-deregulation-of-cell-death-control-genes
#17
Sun Young Kim, Yun Shang, Se-Hwan Joo, Seong-Ki Kim, Kyoung Hee Nam
Since the BRI1-Associated Receptor Kinase 1 (BAK1) was firstly identified as a co-receptor of BRI1 that mediates brassinosteroids (BR) signaling, the functional roles of BAK1, as a versatile co-receptor for various ligand-binding leucine-rich repeat (LRR)-containing receptor-like kinase (RLKs), are being extended to involvement with plant immunity, cell death, stomatal development and ABA signaling in plants. During more than a decade of research on the BAK1, it has been known that transgenic Arabidopsis plants overexpressing BAK1 tagged with various reporters do not fully represent its natural functions...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28124254/probing-activation-and-deactivation-of-the-brassinosteroid-insensitive1-receptor-kinase-by-immunoprecipitation
#18
Sara Martins, Grégory Vert, Yvon Jaillais
Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124252/identification-of-brassinosteroid-signaling-complexes-by-coimmunoprecipitation-and-mass-spectrometry
#19
Walter van Dongen, Luc van Heerde, Sjef Boeren, Sacco C de Vries
A combination of coimmunoprecipitation (coIP) of tagged proteins followed by protein identification and quantitation using Liquid Chromatography Mass Spectrometry/Mass Spectrometry (LCMS/MS) has proven to be a reliable method to qualitatively characterize membrane-bound receptor complexes from plants. Success depends on a range of parameters, such as abundance and stability of the complex and functionality of the tagged receptors, efficiency of the protein complex isolation procedure, MS equipment, and analysis software in use...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124251/analysis-of-in-vitro-dna-interactions-of-brassinosteroid-controlled-transcription-factors-using-electrophoretic-mobility-shift-assay
#20
Simon J Unterholzner, Wilfried Rozhon, Brigitte Poppenberger
Most signaling cascades ultimately lead to changes in gene expression by modulating the activity of transcription factors (TFs). The electrophoretic mobility shift assay (EMSA) is a simple but powerful in vitro method for investigation of specific protein-DNA interactions. It makes use of the fact that protein-DNA complexes have a lower electrophoretic mobility in gels than free DNA has. The application of labeled probes in combination with unlabeled competitors allows investigation of DNA-binding specificity and identification of binding motifs with single base-pair resolution...
2017: Methods in Molecular Biology
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