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https://www.readbyqxmd.com/read/28548820/preparation-of-microcapsules-containing-benzoyl-peroxide-initiator-with-gelatin-gum-arabic-polyurea-formaldehyde-shell-and-evaluating-their-storage-stability
#1
Maryam Raeesi, S Mojtaba Mirabedini, Ramin R Farnood
This work involves the optimized preparation and characterization of microcapsules which contain benzoyl peroxide (BPO) dispersed in dibutyl phthalate (DBP) with gelatin-gum arabic (Gel-GA)/Polyurea-formaldehyde (PUF) shell. The microcapsules were prepared in two steps using complex coacervation and in situ polymerization techniques, respectively, at various mixing speeds and different core:shell ratios. The scanning electron microscopy (SEM), optical microscopy, and Fourier transform infrared (FTIR) spectroscopy were used for characterization of prepared microcapsules...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28547585/aio-casilio-a-robust-crispr-cas9-pumilio-system-for-chromosome-labeling
#2
Shuxian Zhang, Zhan Song
The biological function of chromatin bases on its spatial organization and dynamic activities in different situations. Labeling and tracing of genomic sequences has been a huge challenge in studying the spatial dynamics of chromatin. We reported the development of 'all-in-one Casilio system (Aio-Casilio)', a new system that enables the labeling of endogenous genomic loci. The Aio-Casilio system consists of the dCas9 protein, mClover fused with the Pumilio and FBF proteins RNA-binding domain (PUF domain) and an U6-sgRNA appended with multiple PUF-binding site(s)...
May 25, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28545217/a-miniature-bird-borne-passive-air-sampler-for-monitoring-halogenated-flame-retardants
#3
Manon Sorais, Ali Rezaei, Joseph O Okeme, Miriam L Diamond, Ricardo Izquierdo, Jean-François Giroux, Jonathan Verreault
Birds have been used intensively as biomonitors of halogenated flame retardants (HFRs), and several studies have reported elevated tissue concentrations and inter-individual variability for these contaminants. While diet is known to be an important exposure pathway for HFRs in birds, it has been suggested that exposure through air may represent an underestimated source of HFRs for certain species. However, a method was not available for measuring the atmospheric exposure of individual birds to HFRs or other semi-volatile contaminants...
May 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28525914/cigarette-filter-ventilation-and-its-relationship-to-increasing-rates-of-lung-adenocarcinoma
#4
Min-Ae Song, Neal L Benowitz, Micah Berman, Theodore M Brasky, K Michael Cummings, Dorothy K Hatsukami, Catalin Marian, Richard O'Connor, Vaughan W Rees, Casper Woroszylo, Peter G Shields
The 2014 Surgeon General's Report on smoking and health concluded that changing cigarette designs have caused an increase in lung adenocarcinomas, implicating cigarette filter ventilation that lowers smoking machine tar yields. The Food and Drug Administration (FDA) now has the authority to regulate cigarette design if doing so would improve public health. To support a potential regulatory action, two weight-of-evidence reviews were applied for causally relating filter ventilation to lung adenocarcinoma. Published scientific literature (3284 citations) and internal tobacco company documents contributed to causation analysis evidence blocks and the identification of research gaps...
December 1, 2017: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28518098/engineering-artificial-factors-to-specifically-manipulate-alternative-splicing-in-human-cells
#5
Huan-Huan Wei, Yuanlong Liu, Yang Wang, Qianyun Lu, Xuerong Yang, Jiefu Li, Zefeng Wang
The processing of most eukaryotic RNAs is mediated by RNA Binding Proteins (RBPs) with modular configurations, including an RNA recognition module, which specifically binds the pre-mRNA target and an effector domain. Previously, we have taken advantage of the unique RNA binding mode of the PUF domain in human Pumilio 1 to generate a programmable RNA binding scaffold, which was used to engineer various artificial RBPs to manipulate RNA metabolism. Here, a detailed protocol is described to construct Engineered Splicing Factors (ESFs) that are specifically designed to modulate the alternative splicing of target genes...
April 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28514854/a-new-approach-for-the-synthesis-of-nano-zirconia-fortified-microcapsules
#6
Chia-Chen Li, Dzu-How Yu, Shinn-Jen Chang, Jia-Wei Chen
Robust poly(urea-formaldehyde) (PUF) microcapsules with composite shells comprising zirconia (ZrO2) nanopowder incorporated in PUF were fabricated via a novel and facile one-pot synthesis. ZrO2 nanopowder was chosen because it owns one of the highest mechanical strength among ceramics. The nanopowder was pre-dispersed in the core material to combine encapsulation and fortification into a single process. In the core, the well-dispersed nanopowder migrated to the interface, where PUF polymerization took place...
May 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28449666/nicotine-aerosol-particles-carbonyls-and-volatile-organic-compounds-in-tobacco-and-menthol-flavored-e-cigarettes
#7
Mi-Sun Lee, Ryan F LeBouf, Youn-Suk Son, Petros Koutrakis, David C Christiani
BACKGROUND: We aimed to assess the content of electronic cigarette (EC) emissions for five groups of potentially toxic compounds that are known to be present in tobacco smoke: nicotine, particles, carbonyls, volatile organic compounds (VOCs), and trace elements by flavor and puffing time. METHODS: We used ECs containing a common nicotine strength (1.8%) and the most popular flavors, tobacco and menthol. An automatic multiple smoking machine was used to generate EC aerosols under controlled conditions...
April 27, 2017: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/28414730/elements-including-metals-in-the-atomizer-and-aerosol-of-disposable-electronic-cigarettes-and-electronic-hookahs
#8
Monique Williams, Krassimir Bozhilov, Sanjay Ghai, Prue Talbot
OBJECTIVE: Our purpose was to quantify 36 inorganic chemical elements in aerosols from disposable electronic cigarettes (ECs) and electronic hookahs (EHs), examine the effect of puffing topography on elements in aerosols, and identify the source of the elements. METHODS: Thirty-six inorganic chemical elements and their concentrations in EC/EH aerosols were determined using inductively coupled plasma optical emission spectroscopy, and their source was identified by analyzing disassembled atomizers using scanning electron microscopy and energy dispersive X-ray spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28391086/adolescent-smoking-experimentation-as-a-predictor-of-daily-cigarette-smoking
#9
James D Sargent, Joy Gabrielli, Alan Budney, Samir Soneji, Thomas A Wills
OBJECTIVE: The utility of studying substance use during early adolescence depends on how well indicies of lower-level experimentation predict the development of substance use problems. We examined associations between experimental cigarette use at T1, recanting of use 8 months later (T2), and daily smoking at 2 years (T4). METHODS: Longitudinal telephone survey of 6522 US youth aged 10-14, examining lifetime cigarette smoking (none, just puffing, 1-19, 20-100, >100) and recanting (i...
June 1, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28390787/biodegradation-and-kinetic-study-of-benzene-in-bioreactor-packed-with-puf-and-alginate-beads-and-immobilized-with-bacillus-sp-m3
#10
M K Kureel, S R Geed, B S Giri, B N Rai, R S Singh
Benzene removal in free and immobilized cells on polyurethane foam (PUF) and polyvinyl alcohol (PVA)-alginate beads was studied using an indigenous soil bacterium Bacillus sp. M3 isolated from petroleum-contaminated soil. The important process parameters (pH, temperature and inoculums size) were optimized and found to be 7, 37°C and 6.0×10(8)CFU/mL, respectively. Benzene removals were observed to be 70, 84 and 90% within 9days in a free cell, immobilized PVA-alginate beads and PUF, respectively under optimum operating conditions...
March 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28375254/initial-tamper-tests-of-novel-tamper-indicating-optical-physical-unclonable-functions
#11
Benjamin R Anderson, Ray Gunawidjaja, Hergen Eilers
Optical physical unclonable functions (O-PUFs) are materials containing a large number of randomly distributed degrees of freedom that can be probed optically. We develop tamper-indicating O-PUF seals using polyurethane adhesives with dispersed nanoparticles, where authentication is performed by use of wavefront-shaping controlled reflection. We test the material's and authentication method's tamper-indicating ability using tampering attacks including: mechanical, thermal, and chemical attacks, with the results demonstrating the material/method's robust tamper-indicating ability...
April 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/28365636/image-of-the-month-all-puffed-out-a-case-of-crack-lung
#12
Aryeh Greenberg, Kathryn Stammers, Ian Moonsie, Ricardo J José
No abstract text is available yet for this article.
April 2017: Clinical Medicine: Journal of the Royal College of Physicians of London
https://www.readbyqxmd.com/read/28362040/alternating-air-medium-exposure-in-rotating-bioreactors-optimizes-cell-metabolism-in-3d-novel-tubular-scaffold-polyurethane-foams
#13
Claudia Tresoldi, Ilaria Stefani, Gaia Ferracci, Serena Bertoldi, Alessandro F Pellegata, Silvia Farè, Sara Mantero
BACKGROUND: In vitro dynamic culture conditions play a pivotal role in developing engineered tissue grafts, where the supply of oxygen and nutrients, and waste removal must be permitted within construct thickness. For tubular scaffolds, mass transfer is enhanced by introducing a convective flow through rotating bioreactors with positive effects on cell proliferation, scaffold colonization and extracellular matrix deposition. We characterized a novel polyurethane-based tubular scaffold and investigated the impact of 3 different culture configurations over cell behavior: dynamic (i) single-phase (medium) rotation and (ii) double-phase exposure (medium-air) rotation; static (iii) single-phase static culture as control...
March 27, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28342994/persistent-organic-pollutants-pops-in-the-atmosphere-of-coastal-areas-of-the-ross-sea-antarctica-indications-for-long-term-downward-trends
#14
Karla Pozo, Tania Martellini, Simonetta Corsolini, Tom Harner, Victor Estellano, Petr Kukučka, Marie D Mulder, Gerhard Lammel, Alessandra Cincinelli
Passive air samplers were used to evaluate long-term trends and spatial distribution of trace organic compounds in Antarctica. Duplicate PUF disk samplers were deployed at six automatic weather stations in the coastal area of the Ross sea (East Antarctica), between December 2010 and January 2011, during the XXVI Italian Scientific Research Expedition. Among the investigated persistent organic compounds, Hexachlorobenzene was the most abundant, with air concentrations ranging from 0.8 to 50 pg m(-3). In general, the following decreasing concentration order was found for the air samples analyzed: HCB > PeCB > PCBs > DDTs > HCHs...
July 2017: Chemosphere
https://www.readbyqxmd.com/read/28336095/chlorinated-pesticides-and-natural-brominated-anisoles-in-air-at-three-northern-baltic-stations
#15
Terry F Bidleman, Hjalmar Laudon, Olle Nygren, Staffan Svanberg, Mats Tysklind
Polyurethane foam (PUF) disk passive samplers were deployed at one inland and two island locations in the Bothnian Bay region of the northern Baltic Sea. Uptake was linear over 81-147 d and a temperature range of -2.6 to 14.2 °C for organochlorine pesticides (OCPs) and current-use pesticides (CUPs) having log KOA ≥9 at ambient temperatures. Partial saturation of the PUF disks occurred for the more volatile OCPs hexachlorocyclohexanes (HCHs) and hexachlorobenzene (HCB), and for bromoanisoles (BAs), which are products of bromophenols released by natural and anthropogenic sources...
March 20, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28332915/effects-of-sampling-interval-on-the-passive-air-sampling-of-atmospheric-pcbs-levels
#16
Ahmet Egemen Sakin, Fatma Esen, Yucel Tasdemir
In this study, we collected Polychlorinated biphenyls (PCBs) using passive air samplers (PASs), between February 4, 2013 and February 2, 2014, with the sampling periods ranging from 10 to 60 days. The samples were collected with PASs that contained polyurethane foam (PUF). With these samples, 87 PCB congeners were analyzed. Sampling coefficient (R) values for the four seasons were calculated using both the high volume air sampler (HVAS) and PAS samples collected with the same time interval. The average of the annual concentrations of 87 PCB congeners, calculated using the R values specific to this study, was 234 ± 175 pg/m(3)...
March 23, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28331203/the-role-of-axonal-kv1-channels-in-ca3-pyramidal-cell-excitability
#17
Sylvain Rama, Mickael Zbili, Aurélie Fékété, Mónica Tapia, Maria José Benitez, Norah Boumedine, Juan José Garrido, Dominique Debanne
Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330863/bacterial-virulence-phenotypes-of-escherichia-coli-and-host-susceptibility-determine-risk-for-urinary-tract-infections
#18
Henry L Schreiber, Matt S Conover, Wen-Chi Chou, Michael E Hibbing, Abigail L Manson, Karen W Dodson, Thomas J Hannan, Pacita L Roberts, Ann E Stapleton, Thomas M Hooton, Jonathan Livny, Ashlee M Earl, Scott J Hultgren
Urinary tract infections (UTIs) are caused by uropathogenic Escherichia coli (UPEC) strains. In contrast to many enteric E. coli pathogroups, no genetic signature has been identified for UPEC strains. We conducted a high-resolution comparative genomic study using E. coli isolates collected from the urine of women suffering from frequent recurrent UTIs. These isolates were genetically diverse and varied in their urovirulence, that is, their ability to infect the bladder in a mouse model of cystitis. We found no set of genes, including previously defined putative urovirulence factors (PUFs), that were predictive of urovirulence...
March 22, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28326514/complete-response-to-acupuncture-therapy-in-female-patients-with-refractory-interstitial-cystitis-bladder-pain-syndrome
#19
Mehmet Giray Sönmez, Betül Kozanhan
OBJECTIVES: Interstitial Cystitis/Bladder Pain syndrome (IC/BPS) is a considerable issue in urology and gynecology and unfortunately, the treatment options recommended are not fully efficient. Therefore, in this study we aimed to determine the effectiveness of acupuncture treatment in patients with refractory IC/BPS. MATERIAL AND METHODS: 12 refractory IC/BPS female patients received ten sessions of acupuncture twice a week. The visual analog score (VAS), interstitial cystitis symptom index (ICSI), interstitial cystitis problem index (ICPI), O'Leary-Saint symptom score (OSS), Patient Health Questionnaire (PHQ9), Pelvic pain and urgency & frequency patient symptom scale tests (PUF) and maximum voided volume (MVV) was completed in 1st, 3rd, 6th and 12th months following the treatment...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28320951/recurrent-rewiring-and-emergence-of-rna-regulatory-networks
#20
Daniel Wilinski, Natascha Buter, Andrew D Klocko, Christopher P Lapointe, Eric U Selker, Audrey P Gasch, Marvin Wickens
Alterations in regulatory networks contribute to evolutionary change. Transcriptional networks are reconfigured by changes in the binding specificity of transcription factors and their cognate sites. The evolution of RNA-protein regulatory networks is far less understood. The PUF (Pumilio and FBF) family of RNA regulatory proteins controls the translation, stability, and movements of hundreds of mRNAs in a single species. We probe the evolution of PUF-RNA networks by direct identification of the mRNAs bound to PUF proteins in budding and filamentous fungi and by computational analyses of orthologous RNAs from 62 fungal species...
April 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
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