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https://www.readbyqxmd.com/read/28231673/-changes-of-resistant-phenotype-and-crispr-cas-system-of-four-shigella-strains-passaged-for-90-times-without-antibiotics
#1
B Zhang, L J Hong, G C Duan, W J Liang, H Y Yang, Y L Xi
Objective: To explore the stability of resistant phenotypes and changes of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) gene system on four Shigella strains in the absence of antibiotics. Methods: Four clinical isolated Shigella strains that resistant to different antibiotics were consecutive passaged for 90 times without antibiotics. Agar dilution method was used to determine the minimum inhibitory concentration of Shigella strains. After sequence analysis with PCR, CRISPR Finder and Clustal X 2...
February 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28230771/antibiotic-resistance-determinant-focused-acinetobacter-baumannii-vaccine-designed-using-reverse-vaccinology
#2
Zhaohui Ni, Yan Chen, Edison Ong, Yongqun He
As one of the most influential and troublesome human pathogens, Acinetobacter baumannii (A. baumannii) has emerged with many multidrug-resistant strains. After collecting 33 complete A. baumannii genomes and 84 representative antibiotic resistance determinants, we used the Vaxign reverse vaccinology approach to predict classical type vaccine candidates against A. baumannii infections and new type vaccine candidates against antibiotic resistance. Our genome analysis identified 35 outer membrane or extracellular adhesins that are conserved among all 33 genomes, have no human protein homology, and have less than 2 transmembrane helices...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28230084/highly-potent-antimicrobial-peptides-from-n-terminal-membrane-binding-region-of-e-coli-mreb
#3
Karabi Saikia, Yalavarthi Durga Sravani, Vibin Ramakrishnan, Nitin Chaudhary
Microbial pathogenesis is a serious health concern. The threat escalates as the existing conventional antimicrobials are losing their efficacy against the evolving pathogens. Peptides hold promise to be developed into next-generation antibiotics. Antimicrobial peptides adopt amphipathic structures that could selectively bind to and disrupt the microbial membranes. Interaction of proteins with membranes is central to all living systems and we reasoned that the membrane-binding domains in microbial proteins could be developed into efficient antimicrobials...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229615/bacteremia-with-raoultella-planticola-in-the-setting-of-acute-pancreatitis-complicated-with-acute-cholangitis
#4
Esther Merino Rodríguez, Susana Rebolledo Olmedo, Joaquín Miquel Plaza
The bacterium Raoultella planticola (R planticola) is a rare pathogen in humans. We report a case of mild acute pancreatitis (MAP) of biliary origin with cholangitis and bacteremia with R planticola in association with pancreatic panniculitis (PP). A 55-year-old woman with rheumatic multiple valvulopathy presented with epigastric pain radiating to the back in a belt-like fashion for 48 hours, jaundice, choluria, acholia, and fever (38.2oC). Lab tests showed: amylase 2126 U/L, ALT 124 U/L, AST 169 U/L, GGT 3548 U/L, AP 1516 U/L, TBil 12...
February 23, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/28229558/microbial-diversity-of-a-full-scale-uasb-reactor-applied-to-poultry-slaughterhouse-wastewater-treatment-integration-of-16s-rrna-gene-amplicon-and-shotgun-metagenomic-sequencing
#5
Tiago Palladino Delforno, Gileno Vieira Lacerda Júnior, Melline F Noronha, Isabel K Sakamoto, Maria Bernadete A Varesche, Valéria M Oliveira
The 16S rRNA gene amplicon and whole-genome shotgun metagenomic (WGSM) sequencing approaches were used to investigate wide-spectrum profiles of microbial composition and metabolic diversity from a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment. The data were generated by using MiSeq 2 × 250 bp and HiSeq 2 × 150 bp Illumina sequencing platforms for 16S amplicon and WGSM sequencing, respectively. Each approach revealed a distinct microbial community profile, with Pseudomonas and Psychrobacter as predominant genus for the WGSM dataset and Clostridium and Methanosaeta for the 16S rRNA gene amplicon dataset...
February 23, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28229435/design-and-pharmacodynamics-of-recombinant-nz2114-histidine-mutants-with-improved-activity-against-methicillin-resistant-staphylococcus-aureus
#6
Huixian Chen, Ruoyu Mao, Da Teng, Xiumin Wang, Ya Hao, Xingjun Feng, Jianhua Wang
NZ2114 is a promising candidate for therapeutic application owing to its potent activity to Staphylococcus aureus. Our objective was to identify NZ2114 derivatives with improved activity through substitution of His16 and His18 with residues Arginine and Lysine. Eight mutants were designed and expressed in Pichia pastoris X-33 via pPICZαA. Five of them exhibited strong antimicrobial activity against S. aureus at low minimal inhibitory concentrations (MICs) of 0.057-0.454 μM. Among them, H1, H2, and H3 showed ideal pharmacodynamic effects on methicillin-resistant S...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28229218/microbial-flora-and-resistance-in-ophthalmology-a-review
#7
REVIEW
Andrzej Grzybowski, Piotr Brona, Stephen Jae Kim
Antibiotic resistance in systemic infection is well-researched and well-publicized. Much less information is available on the resistance of normal ocular microbiome and that of ophthalmic infections. An understanding of the distribution of ocular microorganisms may help us in tailoring our empiric treatment, as well as in choosing effective pre-, peri- and postoperative management, to achieve the best results for patients. This study aims to summarize and review the available literature on the subject of normal ocular flora and its resistance, as well as the broader topic of antibiotic resistance in ophthalmology...
February 22, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28229205/-spectrum-of-pathogens-in-postoperative-complications-of-visceral-surgery-the-problem-of-multidrug-resistance
#8
A Kramer, J Pochhammer, P Walger, U Seifert, M Ruhnke, J C Harnoss
In general surgery the etiology of surgical site infections has not significantly changed over the last 30 years. Gram-positive bacteria, e.g. coagulase negative staphylococci (CNS), Staphylococcus aureus and Enterococcus spp. as well as Gram-negative bacteria, e.g. Escherichia coli, Enterobacter spp., Klebsiella spp. and Pseudomonas aeruginosa, are the most common findings. Although in general surgery 10% of the S. aureus causing postoperative wound infections were methicillin resistant (MRSA), no cases of multidrug-resistant Gram-negative (MRGN) bacteria were reported...
February 22, 2017: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
https://www.readbyqxmd.com/read/28229046/genome-sequence-of-three-psychrobacter-sp-strains-with-potential-applications-in-bioremediation
#9
Aide Lasa, Jesús L Romalde
To date, the genus Psychrobacter consists of 37 recognized species isolated from different sources, however they are more frequently found in cold and other non-polar environments of low water activity. Some strains belonging to the genus have shown different enzymatic activities with potential applications in bioremediation or food industry. In the present study, the whole genome sequences of three Psychrobacter-like strains (C 20.9, Cmf 22.2 and Rd 27.2) isolated from reared clams in Galicia (Spain) are described...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28229018/-genomic-data-mining-of-the-marine-actinobacteria-streptomyces-sp-h-kf8-unveils-insights-into-multi-stress-related-genes-and-metabolic-pathways-involved-in-antimicrobial-synthesis
#10
Agustina Undabarrena, Juan A Ugalde, Michael Seeger, Beatriz Cámara
Streptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs...
2017: PeerJ
https://www.readbyqxmd.com/read/28228089/genotypic-characterization-of-vancomycin-resistant-enterococcus-spp-in-tertiary-center-iran
#11
Shervin Shokouhi, Ilad Alavi Darazam, Matin Rouhani, Mahdiyeh Ghasemnejad
BACKGROUND: Enterococci infection rate, mortality and morbidity has been increased in recent decades accompanied by progressive emerging antimicrobial resistance. Vancomycin-resistant enterococci (VRE), important pathogen in hospitalized patients, have distinct antibiotic susceptibility to glycopeptides that varied genetically and can influence in choosing therapeutic agents. In this study, we aimed to determine the patterns of antimicrobial resistance according to the genotypic variations in VREs...
February 10, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28228075/challenges-in-dermal-delivery-of-therapeutic-antimicrobial-protein-and-peptides
#12
Rohan Aminbhai Lalani, Ambikanandan Misra, Jitendra Amrutiya, Hinal Patel, Priyanka Bhatt, Vivek Patel
BACKGROUND: Protein and peptides in biological system form an important part of innate immune system and are being explored for potential use in various diseases as therapeutics. Importance of proteins and peptides as a new class of antimicrobial agents have boosted research in the field of biotechnology as potential alternative to antibiotic agents. OBJECTIVE: Protein and peptides antimicrobial as a therapeutics class are structurally diverse and exhibit potent activity against microbes by various mechanisms...
February 22, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28225917/oxacillin-magnetically-targeted-for-the-treatment-of-methicillin-resistant-s-aureus-infection-in-rats
#13
Juliana Fernandes de Carvalho, Ítalo Medeiros de Azevedo, Keyla Borges Ferreira Rocha, Aldo Cunha Medeiros, Artur da Silva Carriço
Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles...
January 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28225903/sphygmomanometers-and-thermometers-as-potential-fomites-of-staphylococcus-haemolyticus-biofilm-formation-in-the-presence-of-antibiotics
#14
Bruna Pinto Ribeiro Sued, Paula Marcele Afonso Pereira, Yuri Vieira Faria, Juliana Nunes Ramos, Vanessa Batista Binatti, Kátia Regina Netto Dos Santos, Sérgio Henrique Seabra, Raphael Hirata, Verônica Viana Vieira, Ana Luíza Mattos-Guaraldi, José Augusto Adler Pereira
BACKGROUND: The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES: In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated...
March 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28225897/sphygmomanometers-and-thermometers-as-potential-fomites-of-staphylococcus-haemolyticus-biofilm-formation-in-the-presence-of-antibiotics
#15
Bruna Pinto Ribeiro Sued, Paula Marcele Afonso Pereira, Yuri Vieira Faria, Juliana Nunes Ramos, Vanessa Batista Binatti, Kátia Regina Netto Dos Santos, Sérgio Henrique Seabra, Raphael Hirata, Verônica Viana Vieira, Ana Luíza Mattos-Guaraldi, José Augusto Adler Pereira
BACKGROUND: The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES: In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated...
February 16, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28225711/reactive-oxygen-therapy-a-novel-therapy-in-soft-tissue-infection
#16
Matthew Dryden
PURPOSE OF REVIEW: The global burden of disease in skin and soft tissue lesions is enormous. Many chronic, poorly healing lesions get treated with antibiotics despite the lack of evidence for long-term antibiotics. There is a global antibiotic resistance crisis driven largely by inappropriate use of large volumes of antibiotics. One solution is to reduce the selection pressure on bacteria by reducing the volume of antibiotic use in medicine, agriculture and the environment. There are few novel antimicrobials...
April 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28225264/fatty-acid-comprising-lysine-conjugates-anti-mrsa-agents-that-display-in-vivo-efficacy-by-disrupting-biofilms-with-no-resistance-development
#17
Mohini Mohan Konai, Jayanta Haldar
Methicillin-resistant Staphylococcus aureus (MRSA) have developed resistance to antibiotics of last resort such as vancomycin, linezolid and daptomycin. Additionally, their biofilm forming capability has set an alarming situation in the treatment of bacterial infections. Herein we report the potency of fatty acid comprising lysine conjugates as novel anti-MRSA agents, which were not only capable of killing growing planktonic MRSA at low concentration (MIC = 3.1-6.3 µg/mL), but also displayed potent activity against non-dividing stationary phase cells...
February 22, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28225263/bactericidal-effect-of-gold-chitosan-nanocomposites-in-co-culture-models-of-pathogenic-bacteria-and-human-macrophages
#18
Gracia Mendoza, Anna Regiel-Futyra, Vanesa Andreu, Victor Sebastian, Agnieszka Kyzioł, Grazyna Stochel, Manuel Arruebo
Pathogenic bacteria are able to develop resistance mechanisms to avoid the antimicrobial potential of antibiotics becoming an increasing problem for the healthcare systems. The search for more effective and selective antimicrobial materials, though not harmful to mammalian cells, seems imperative. Herein we propose the use of gold-chitosan nanocomposites as effective bactericidal materials avoiding the damage to human cells. Nanocomposites were obtained taking advantage of the reductive and stabilizing action of chitosan solutions on two different gold precursor concentrations...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28224049/the-role-of-topical-antiseptic-agents-within-antimicrobial-stewardship-strategies-for-prevention-and-treatment-of-surgical-site-and-chronic-open-wound-infection
#19
Christopher D Roberts, David J Leaper, Ojan Assadian
Scope and Significance: The topical use of antiseptics for wound care has a role in an antimicrobial stewardship strategy. However, the details of this role need clarification. Further clinical research into the use of topical antiseptics in wound care would lower the risk of furthering antibiotic resistance and contribute to more effective antibiotic use. As part of this research, experimental and surveillance data are needed on the resistance and tolerance patterns associated with topical antiseptic use in wound infections...
February 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28224028/evaluation-of-antimicrobial-susceptibility-and-integron-carriage-in-helicobacter-pylori-isolates-from-patients
#20
Mehdi Goudarzi, Mohsen Heidary, Mehdi Azad, Maryam Fazeli, Hossein Goudarzi
AIM: The purpose of this study was to determine the antibiotic susceptibility pattern and distribution of integron in H. pylori isolates collected from patients referred to private health care centers in Tehran, Iran. BACKGROUND: Antibiotic resistance is the main reason for failure of Helicobacter pylori therapy. Integrons as genetic reservoirs play main roles in the dissemination of antimicrobial resistance gene. METHODS: During a 12-month cross-sectional study period, 65 H...
December 2016: Gastroenterology and Hepatology From Bed to Bench
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