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https://www.readbyqxmd.com/read/27909726/microrna%C3%A2-21-promotes-migration-and-invasion-of-glioma-cells-via-activation-of-sox2-and-%C3%AE-%C3%A2-catenin-signaling
#1
Guoxuan Luo, Wentao Luo, Xiaohui Sun, Jinzhi Lin, Mo Wang, Yang Zhang, Weishi Luo, Yong Zhang
The expression of microRNA 21 (miR-21) has been reported to be upregulated in various types of cancer, including malignant gliomas. However, its functions and mechanisms in glioma remain to be fully elucidated. The present study established miRNA‑21 overexpression and knockdown cell lines using SRY‑box 2 (Sox2) small interfering RNA (siRNA) to knockdown expression and Sox2 cDNA was cloned into pcDNA 3.1 mammalian expression vector for ectopic expression. BIO and XAV‑939 were used for β‑catenin signaling activation and knockdown, respectively...
November 28, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27900021/inhibition-of-pttg1-expression-by-microrna-suppresses-proliferation-and-induces-apoptosis-of-malignant-glioma-cells
#2
Xing Su, Jianguo Chen, Lanchun Ni, Wei Shi, Jinlong Shi, Xiaojiang Liu, Yu Zhang, Peipei Gong, Hui Zhu, Qingfeng Huang
The present study aimed to investigate the role of pituitary tumor-transforming gene 1 (PTTG1) in the proliferation, invasion and apoptosis of human malignant glioma U251 cells. Firstly, 2 microRNAs (miRNAs) targeting PTTG1 messenger (m)RNA were ligated into a pcDNA6.2-GW/EmGFP-miR expression vector. The recombinant plasmids, miRNA-1 and miRNA-2 (miR-2), were transfected into U251 cells using the liposome method. PTTG1 mRNA and protein levels were evaluated using quantitative polymerase chain reaction and western blot analysis...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27893811/the-inhibition-of-microrna-128-on-igf-1-activating-mtor-signaling-involves-in-temozolomide-induced-glioma-cell-apoptotic-death
#3
Peng-Hsu Chen, Chia-Hsiung Cheng, Chwen-Ming Shih, Kuo-Hao Ho, Cheng-Wei Lin, Chin-Cheng Lee, Ann-Jeng Liu, Cheng-Kuei Chang, Ku-Chung Chen
Temozolomide (TMZ), an alkylating agent of the imidazotetrazine series, is a first-line chemotherapeutic drug used in the clinical therapy of glioblastoma multiforme, the most common and high-grade primary glioma in adults. Micro (mi)RNAs, which are small noncoding RNAs, post-transcriptionally regulate gene expressions and are involved in gliomagenesis. However, no studies have reported relationships between TMZ and miRNA gene regulation. We investigated TMZ-mediated miRNA profiles and its molecular mechanisms underlying the induction of glioma cell death...
2016: PloS One
https://www.readbyqxmd.com/read/27880937/prognostic-relevance-of-mirna-155-methylation-in-anaplastic-glioma
#4
Maximilian Georg Schliesser, Rainer Claus, Thomas Hielscher, Christiane Grimm, Dieter Weichenhan, Jonas Blaes, Benedikt Wiestler, Peter Hau, Johannes Schramm, Felix Sahm, Elisa K Weiß, Markus Weiler, Constance Baer, Friederike Schmidt-Graf, Gabriele Schackert, Manfred Westphal, Anne Hertenstein, Patrick Roth, Norbert Galldiks, Christian Hartmann, Torsten Pietsch, Joerg Felsberg, Guido Reifenberger, Michael Christoph Sabel, Frank Winkler, Andreas von Deimling, Christoph Meisner, Peter Vajkoczy, Michael Platten, Michael Weller, Christoph Plass, Wolfgang Wick
The outcome of patients with anaplastic gliomas varies considerably depending on single molecular markers, such as mutations of the isocitrate dehydrogenase (IDH) genes, as well as molecular classifications based on epigenetic or genetic profiles. Remarkably, 98% of the RNA within a cell is not translated into proteins. Of those, especially microRNAs (miRNAs) have been shown not only to have a major influence on physiologic processes but also to be deregulated and prognostic in malignancies.To find novel survival markers and treatment options we performed unbiased DNA methylation screens that revealed 12 putative miRNA promoter regions with differential DNA methylation in anaplastic gliomas...
November 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27871300/pi3-kinase-pathway-regulated-mirnome-in-glioblastoma-identification-of-mir-326-as-a-tumour-suppressor-mirna
#5
Zahid Nawaz, Vikas Patil, Yashna Paul, Alangar S Hegde, Arimappamagan Arivazhagan, Vani Santosh, Kumaravel Somasundaram
BACKGROUND: Glioblastomas (GBM) continue to remain one of the most dreaded tumours that are highly infiltrative in nature and easily preclude comprehensive surgical resection. GBMs pose an intricate etiology as they are being associated with a plethora of genetic and epigenetic lesions. Misregulation of the PI3 kinase pathway is one of the most familiar events in GBM. While the PI3 kinase signalling regulated pathways and genes have been comprehensively studied, its impact on the miRNome is yet to be explored...
November 21, 2016: Molecular Cancer
https://www.readbyqxmd.com/read/27862991/down-regulation-of-microrna-320d-predicts-poor-overall-survival-and-promotes-the-growth-and-invasive-abilities-in-glioma
#6
Chong-Zhen Qin, Qiao-Li Lv, Yan-Tao Yang, Jing-Min Zhang, Xiao-Jian Zhang, Hong-Hao Zhou
Previous studies have demonstrated that miRNAs play an important role in tumor development and progression. The role of miR-320d has been studied in several cancers except for glioma. The aim of the study was to investigate the expression levels, biological function, and mechanism of miR-320d in glioma. The expression levels of miR-320d were detected in glioma tissues and cell lines (U87 and U251) by RT-qPCR. Cell proliferation, colony formation, apoptosis, cell cycle and transwell assays were performed in glioma cell lines transfected with miR-320d mimics or controls to evaluate the effects of miR-320d in vitro...
November 12, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27810075/co-localized-genomic-regulation-of-mirna-and-mrna-via-dna-methylation-affects-survival-in-multiple-tumor-types
#7
James D Doecke, Ying Wang, Keith Baggerly
Aberrant gene expression in cancer is due in part to irregular patterns of DNA methylation and miRNA target gene down regulation. Using data from The Cancer Genome Atlas (TGCA), we investigated co-localized mRNA, miRNA and DNA methylation data across 15 cancer types, focusing on cases where evidence for direct regulation was strong. Restricting attention to regions where miRNA markers co-localize within a corresponding mRNA transcript, we checked expression data from 2839 samples for 354 mRNAs, 389 miRNAs and 13,809 DNA methylation probes for correlations greater than an absolute 0...
October 2016: Cancer Genetics
https://www.readbyqxmd.com/read/27801803/mir-92a-3p-exerts-various-effects-in-glioma-and-glioma-stem-like-cells-specifically-targeting-cdh1-%C3%AE-catenin-and-notch-1-akt-signaling-pathways
#8
Hang Song, Yao Zhang, Na Liu, Sheng Zhao, Yan Kong, Liudi Yuan
MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to develop novel therapeutic approaches for glioma patients. In the present study, we applied methyl-thiazolyl-tetrazolium (MTT) assay and Transwell assay to measure the proliferation rate and metastatic potential of glioma cells...
October 27, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27796868/effect-of-rs11614913-polymorphism-on-mature-mir196a2-expression-and-its-target-gene-hoxc8-expression-in-human-glioma
#9
M K Sibin, S M Harshitha, K V L Narasingarao, I Bhat Dhananjaya, P Shukla Dhaval, G K Chetan
miRNA polymorphisms are recently identified as a risk factor for various cancers, and it is associated with change in the expression of target genes in vitro. rs11614913 polymorphism in miR196a2 was associated with risk of glioma in Chinese population. In this study, we have evaluated the role of rs11614913 polymorphism and glioma risk in Indian population in 180 cases and controls. Seventy-two glioma tissue-blood pairs were also assessed for mutation in this SNP. Further, the effect of this polymorphism on mature miR196a2 expression and HOXC8 gene expression was analysed in 33 glioma tissue samples with different genotypes...
October 28, 2016: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/27771233/the-emergent-role-of-exosomes-in-glioma
#10
J Gourlay, A P Morokoff, R B Luwor, H-J Zhu, A H Kaye, S S Stylli
Extracellular vesicles (EVs) are known mediators of intercellular communication for both normal and tumour cells. With the capability to transfer nucleic acids, proteins and lipids, EVs are able to influence numerous functional and pathological aspects of both donor and recipient cells. The tumour microenvironment possesses a high level of complex heterogeneity, particularly within the most prominent brain malignancy, glioblastoma multiforme (GBM). This complexity relies on a network-based communication between many different components of the local niche, including the various cell types, stroma, blood vessels, secreted factors and surrounding matrix...
October 19, 2016: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/27748823/microrna-16-inhibits-the-proliferation-migration-and-invasion-of-glioma-cells-by-targeting-sal-like-protein%C3%A2-4
#11
Yu Zhou, Yang Liu, Chao Hu, Yugang Jiang
MicroRNAs (miRNAs or miRs), a class of non-coding RNAs 18-25 nucleotides in length, act as key regulators in the development and malignant progression of various human cancers by modulating the expression of their target genes. Recently, miR‑16 has been demonstrated to be play a role in glioma. However, the regulatory mechanisms of miR‑16 in glioma growth and metastasis remain largely unclear. In the present study, qRT-PCR revealed that miR‑16 was significantly downregulated in 23 glioma tissue specimens compared to 7 normal brain tissue specimens...
October 17, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27705931/mir128-1-inhibits-the-growth-of-glioblastoma-multiforme-and-glioma-stem-like-cells-via-targeting-bmi1-and-e2f3
#12
Zheng-Nan Shan, Rui Tian, Min Zhang, Zhao-Hua Gui, Jing Wu, Min Ding, Xin-Fu Zhou, Jie He
MicroRNA128-1 (miR128-1), as a brain-specific miRNA, is downregulated in glioblastoma multiforme (GBM) and closely associated with the progression of GBM. However, the underlying molecular mechanism of the downregulation and its role in the regulation of tumorigenesis and anticancer drug resistance in GBM remains largely unknown. In the current study,we found that miR128-1 was downregulated in GBM and glioma stem-like cells (GSCs). Intriguingly, treatment with the DNA methylation inhibitors 5-Aza-CdR (Aza) and 4-phenylbutyric acid (PBA) resulted in miR128-1 upregulation in both GBM cells and GSCs...
October 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27698888/mirna-regulation-of-sirtuin-1-expression-in-human-astrocytoma
#13
Sara Giovanna Romeo, Alfredo Conti, Francesca Polito, Chiara Tomasello, Valeria Barresi, Domenico La La Torre, Maria Cucinotta, Flavio Filippo Angileri, Marcello Bartolotta, Rosa Maria Di Giorgio, M'Hammed Aguennouz
Sirtuins are a family of 7 histone deacetylases largely involved in the regulation of cell proliferation, survival and death. The role of sirtuins in tumorigenesis and cancer progression has been previously studied in certain cancer types. Few studies have investigated sirtuin expression in gliomas, with controversial results. The aim of the present study was to investigate the expression of sirtuin-1 (Sirt-1) in diffuse astrocytoma [low grade astrocytoma (LGA)], anaplastic astrocytoma (AA) and glioblastoma multiforme (GBM) and in primary glioma cell lines: PLGAC (primary LGA cells); PAAC (primary AA cells); and PGBMC (primary GBM cells)...
October 2016: Oncology Letters
https://www.readbyqxmd.com/read/27690671/peptides-as-a-therapeutic-avenue-for-nanocarrier-aided-targeting-of-glioma
#14
Mrunal Vitthal Wanjale, G S Vinod Kumar
Very few successful interventions have been possible in glioma therapy owing to its aggressive nature as well as its hindrance of targeted therapy together with the limited access afforded by the blood-brain barrier (BBB). With the advent of nanotechnology based delivery vehicles such as micelles, dendrimers, polymer-based nanoparticles and nanogels, the breach of the BBB has been facilitated. However, there remains the issue of targeted therapy for glioma cells. Peptide-mediated surface modification of nanocarriers serves this purpose, extending the ability to target glioma further than the enhanced permeability and retention effect...
October 3, 2016: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/27682888/mir-548b-inhibits-the-proliferation-and-invasion-of-malignant-gliomas-by-targeting-metastasis-tumor-associated-protein-2
#15
Yunzhi Pan, Wenna Liang, Xiaoyang Zhao, Luping Liu, Yang Qing, Yongmei Li
microRNAs (miRNAs) play important roles in cancer development and progression. In this study, we explored the expression and biological roles of miR-548b in human gliomas. The expression of miR-548b in human glioma tissues and cell lines was examined. Gain-of-function experiments were conducted to determine the roles of miR-548b in glioma cell growth, invasiveness, and tumorigenesis. Bioinformatic analysis and luciferase reporter assays were performed to identify direct target genes for miR-548b. miR-548b was underexpressed in human glioma tissues and cell lines...
September 27, 2016: Neuroreport
https://www.readbyqxmd.com/read/27673330/long-noncoding-rna-mir210hg-as-a-potential-biomarker-for-the-diagnosis-of-glioma
#16
Weijie Min, Dongwei Dai, Jiaqi Wang, Dandan Zhang, Yuhui Zhang, Guosheng Han, Lei Zhang, Chao Chen, Xiulong Li, Yanan Li, Zhijian Yue
BACKGROUND: Glioma remains a diagnostic challenge because of its variable clinical presentation and a lack of reliable screening tools. Long noncoding RNAs (lncRNAs) regulate gene function in a wide range of pathophysiological processes and are therefore emerging biomarkers for prostate cancer, hepatic cancer, and other tumor diseases. However, the effective use of lncRNAs as biomarkers for the diagnosis of glioma remains unproven. METHODS: This study included 42 glioma patients and 10 healthy controls...
2016: PloS One
https://www.readbyqxmd.com/read/27663885/mirna-429-inhibits-astrocytoma-proliferation-and-invasion-by-targeting-bmi1
#17
Gang Peng, Yiwei Liao, Chenfu Shen
Glioblastoma multiforme (GBM), the most common primary brain cancer in adults, is usually the most lethal type of brain tumor. MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that deeply involves with the regulation of gene expression and cellular processes, including proliferation, apoptosis, migration and invasion. The objective of the study is to investigate the effect of miRNA-429 on human glioblastoma tissues and cell lines. miRNA-429 expressions in human glioblastoma, normal brain tissue samples, and human malignant glioma cell lines (U87, U251 and LN229) were compared using reverse transcription-quantitative PCR and western blot methods...
September 23, 2016: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/27652111/prognostic-role-of-microrna-203-in-various-carcinomas-evidence-from-a-meta-analysis-involving-13-studies
#18
REVIEW
Ying Liang, Wenhui Yang, Yanhui Zhu, Yulin Yuan
Growing evidence from recent studies has revealed that microRNA-203 (miR-203) might be an attractive prognostic biomarker for cancer. But controversy still remains. The aim of this meta-analysis was to summarize available evidences and clarify the preliminary predictive value of miR-203 for prognosis in cancer patients. Eligible studies were identified through multiple research strategies in PubMed, EMBASE and Web of Science up to October 2015. Key statistics such as pooled hazard ratios (HR) with 95 % confidence intervals (CIs) were utilized to calculate patient survival...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27648356/mir-186-inhibits-cell-proliferation-of-prostate-cancer-by-targeting-golph3
#19
Xing Hua, Yu Xiao, Wenhai Pan, Meiyun Li, Xiaoxiao Huang, Zexiao Liao, Qi Xian, Lina Yu
Prostate cancer is one of the leading causes of cancer deaths among men, many miRNAs have been demonstrated to play critical role in the progression of prostate cancer, miR-186 suppresses the progression of many tumors, such as bladder cancer and glioma. Previous study shows miR-186 is downregulated in prostate cancer tissues, and is a good prognosis for prostate cancer patients. In this study, we found miR-186 was downregulated in prostate cancer cells and tissues, overexpression of miR-186 inhibited cell proliferation and tumorigenesis in vitro determined by MTT assay, colony formation assay and soft agar growth assay, whereas knockdown of miR-186 reduced these effects...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27629767/the-expression-of-mirna-221-and-mirna-222-in-gliomas-patients-and-their-prognosis
#20
Liang Xue, Yi Wang, Shuyuan Yue, Jianning Zhang
The aim of this study is to explore the expression of microRNA (miRNA)-221 and miRNA-222 in human glioma cells and tissues. The expression of miRNA-221 and miRNA-222 in human glioma cell line U87, U251, A172, LN229 and surgery resected glioma tissues were measured. The survival rate of X-ray (2 Gy) irradiated glioma cells were calculated. 165 cases of glioma patients were recruited successfully; the expression of miRNA-221 and miRNA-222 in their resected tissues were measured. The expression of miRNA-221 and miRNA-222 in cancer tissues were obviously higher than control tissues (normal brain tissue) and control cell (gastric mucosal epithelial cell, GES) (p < 0...
September 15, 2016: Neurological Sciences
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