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https://www.readbyqxmd.com/read/28580919/crystallization-and-x-ray-diffraction-analysis-of-spae-a-basal-pilus-protein-from-the-gut-adapted-lactobacillus-rhamnosus-gg
#1
Arjun K Mishra, Abhin Kumar Megta, Airi Palva, Ingemar von Ossowski, Vengadesan Krishnan
SpaE is the predicted basal pilin subunit in the sortase-dependent SpaFED pilus from the gut-adapted and commensal Lactobacillus rhamnosus GG. Thus far, structural characterization of the cell-wall-anchoring basal pilins has remained difficult and has been limited to only a few examples from pathogenic genera and species. To gain a further structural understanding of the molecular mechanisms that are involved in the anchoring and assembly of sortase-dependent pili in less harmful bacteria, L. rhamnosus GG SpaE for crystallization was produced by recombinant expression in Escherichia coli...
June 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28522396/ultrafast-spectroscopy-on-dna-cleavage-by-endonuclease-in-molecular-crowding
#2
Priya Singh, Susobhan Choudhury, Shreyasi Dutta, Aniruddha Adhikari, Siddhartha Bhattacharya, Debasish Pal, Samir Kumar Pal
The jam-packed intracellular environments differ the activity of a biological macromolecule from that in laboratory environments (in vitro) through a number of mechanisms called molecular crowding related to structure, function and dynamics of the macromolecule. Here, we have explored the structure, function and dynamics of a model enzyme protein DNase I in molecular crowing of polyethylene glycol (PEG; MW 3350). We have used steady state and picosecond resolved dynamics of a well-known intercalator ethidium bromide (EB) in a 20-mer double-stranded DNA (dsDNA) to monitor the DNA-cleavage by the enzyme in absence and presence PEG...
May 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28382323/the-impact-of-diet-liberalization-on-bowel-preparation-for-colonoscopy
#3
James Walter, Gloria Francis, Rebecca Matro, Ramalinga Kedika, Rachael Grosso, Scott W Keith, David Kastenberg
Background and study aims Dietary restrictions are integral to colonoscopy preparation and impact patient satisfaction. Utilizing split-dose, lower-volume polyethylene glycol 3350-electrolyte solution (PEG-ELS), this study compared colon preparation adequacy of a low-residue diet to clear liquids using a validated grading scale. Patients and methods This was a prospective, randomized, single-blinded, single-center non-inferiority study evaluating diet the day prior to outpatient colonoscopy. Subjects were randomized to a Low-Residue diet for breakfast and lunch, or Clears only...
April 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28242427/purification-and-characterization-of-a-collagenase-from-penicillium-sp-ucp-1286-by-polyethylene-glycol-phosphate-aqueous-two-phase-system
#4
Maria Carolina de Albuquerque Wanderley, José Manoel Wanderley Duarte Neto, Wendell Wagner Campos Albuquerque, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima, Sara Isabel da Cruz Silvério, José Luiz de Lima Filho, José António Couto Teixeira, Ana Lúcia Figueiredo Porto
Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including low-processing time, low-cost material and low-energy consumption. The collagenase produced by Penicillium sp...
February 24, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28147222/seventy-years-of-polyethylene-glycols-in-gastroenterology-the-journey-of-peg-4000-and-3350-from-nonabsorbable-marker-to-colonoscopy-preparation-to-osmotic-laxative
#5
EDITORIAL
John S Fordtran, Alan F Hofmann
No abstract text is available yet for this article.
January 29, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28110184/structural-analysis-of-binding-functionality-of-folic-acid-peg-dendrimers-against-folate-receptor
#6
Diana Sampogna-Mireles, Ingrid D Araya-Durán, Valeria Márquez-Miranda, Jesús A Valencia-Gallegos, Fernando D González-Nilo
Dendrimers functionalized with folic acid (FA) are drug delivery systems that can selectively target cancer cells with folate receptors (FR-α) overexpression. Incorporation of polyethylene glycol (PEG) can enhance dendrimers solubility and pharmacokinetics, but ligand-receptor binding must not be affected. In this work we characterized, at atomic level, the binding functionality of conventional site-specific dendrimers conjugated with FA with PEG 750 or PEG 3350 as a linker. After Molecular Dynamics simulation, we observed that both PEG's did not interfere over ligand-receptor binding functionality...
January 6, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/27769928/binding-studies-and-structure-determination-of-the-recombinantly-produced-type-ii-3-dehydroquinate-dehydratase-from-acinetobacter-baumannii
#7
Naseer Iqbal, Mukesh Kumar, Pradeep Sharma, Satya Prakash Yadav, Punit Kaur, Sujata Sharma, Tej P Singh
Dehydroquinase (3-dehydroquinate dehydratase, DHQD, EC 4.2.1.10) catalyzes the conversion of dehydroquinate to dehydroshikimate. DHQD from Acinetobacter baumannii (AbDHQD) was cloned, expressed and purified to homogeneity. The binding studies showed that two compounds quinic acid and citrazinic acid bound to AbDHQD at micromolar concentrations. AbDHQD was crystallized using 30% PEG-3350, 50mM tris-HCl and 1.0M MgSO4 at pH 8.0. Crystals of AbDHQD were stabilized with 25% glycerol for data collection at 100K...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27616074/quality-by-design-qbd-approach-for-design-and-development-of-drug-device-combination-products-a-case-study-on-flunisolide-nasal-spray
#8
Swapnil Sharadkumar Chudiwal, Mohamed Hassan G Dehghan
The objective of the present study was to design and develop drug-device combination product in particular flunisolide nasal spray using quality by design (QbD) approach. Quality target product profile (QTPP) of flunisolide nasal spray was defined and critical quality attributes (CQAs) i.e. viscosity (cp) (Y1) and D50 DSD (µm) (Y2) were identified. Potential risk factors were identified using a fish bone diagram and failure mode effect analysis (FMEA) tools. Plackett Burman and Box Behnken design were used for screening the significant factors and optimizing the variables range respectively...
September 10, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27569713/peg-and-thickeners-a-critical-interaction-between-polyethylene-glycol-laxative-and-starch-based-thickeners
#9
Brian J Carlisle, Garrett Craft, Julie P Harmon, Alina Ilkevitch, Jenik Nicoghosian, Inna Sheyner, Jonathan T Stewart
Clinicians commonly encounter dysphagia and constipation in a skilled nursing population. Increasing the viscosity of liquids, usually with a starch- or xanthan gum-based thickener, serves as a key intervention for patients with dysphagia. We report a newly identified and potentially dangerous interaction between polyethylene glycol 3350 laxative (PEG) and starch-thickened liquids. A patient requiring nectar-thickened liquids became constipated, and medical staff prescribed PEG for constipation. His nurse observed that the thickened apple juice immediately thinned to near-water consistency when PEG was added...
September 1, 2016: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/27550498/anaphylaxis-to-polyethylene-glycol-colyte%C3%A2-in-a-patient-with-diverticulitis
#10
So Hee Lee, Sun Hyuk Hwang, Jin Soo Park, Hae Sim Park, Yoo Seob Shin
Polyethylene glycols (PEGs) are believed to be chemically inert agents, but larger PEG polymers could have immunogenicity. A 39-year-old man was referred to emergency room for loss of consciousness and dyspnea after taking of PEG-3350 (Colyte®). In laboratory findings, the initial serum tryptase level was increased to 91.9 mg/L (normal range: 0.00-11.40 mg/L) without any other laboratory abnormalities. The intradermal test with 10 mg/mL Colyte® showed a 5 × 5 mm wheal, but basophil activation and histamine releasability tests were negative...
October 2016: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/27486340/safety-of-polyethylene-glycol-3350-solution-in-chronic-constipation-randomized-placebo-controlled-trial
#11
Thomas McGraw
PURPOSE: To evaluate the safety and tolerability of aqueous solution concentrate (ASC) of polyethylene glycol (PEG) 3350 in patients with functional constipation. PATIENTS AND METHODS: The patients who met Rome III diagnostic criteria for functional constipation were randomized in this multicenter, randomized, placebo-controlled, single-blind study to receive once daily dose of PEG 3350 (17 g) ASC or placebo solution for 14 days. The study comprised a screening period (visit 1), endoscopy procedure (visits 2 and 3), and followup telephone calls 30 days post-treatment...
2016: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/27425772/-intestinal-cleaning-for-colonoscopy-in-children-effectiveness-adherence-and-adverse-effects-of-schemes-differentiated-by-age
#12
Isabel Miquel, María Eugenia Arancibia, Francisco Alliende, Gloria Ríos, Lorena Rodríguez, Yalda Lucero, Eric Saelzer
Adequate intestinal cleanliness is crucial to achieve optimal colonoscopy performance. Several bowel preparation (BP) schemes have been proposed, but there is still no consensus as regards which is the most suitable in paediatric patients. OBJECTIVE: To describe the effectiveness, adherence, and adverse effects of BP protocols differentiated by age group in paediatric patients subjected to colonoscopy. PATIENTS AND METHOD: Prospective, study that included patients < 18 years subjected to colonoscopy...
April 2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/27303896/putative-thioredoxin-trx1-from-thermosipho-africanus-strain-tcf52b-expression-purification-and-structural-determination-using-s-sad
#13
Naheda Sahtout, Jijin R A Kuttiyatveetil, Michel Fodje, David A R Sanders
Thioredoxin is a small ubiquitous protein that plays a role in many biological processes. A putative thioredoxin, Trx1, from Thermosipho africanus strain TCF52B, which has low sequence identity to its closest homologues, was successfully cloned, overexpressed and purified. The protein was crystallized using the microbatch-under-oil technique at 289 K in a variety of conditions; crystals grown in 0.2 M MgCl2, 0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350, which grew as irregular trapezoids to maximum dimensions of 1...
June 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27295188/the-influence-of-polyethylene-glycol-solution-on-the-dissolution-rate-of-sustained-release-morphine
#14
Michael Hodgman, Michael G Holland, Ulrich Englich, Susan M Wojcik, William D Grant, Erich Leitner
INTRODUCTION: Whole bowel irrigation (WBI) is a management option for overdose of medications poorly adsorbed to activated charcoal, with modified release properties, or for body packers. Polyethylene glycol (PEG) is a mixture of ethylene oxide polymers of varying molecular weight. PEG with an average molecular weight of 3350 g/mol is used for WBI. PEG electrolyte lavage solution has been shown in vitro to hasten the dissolution of acetaminophen. The impact of PEG on the pharmacokinetics of extended release pharmaceuticals is unknown...
December 2016: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/27158544/polyethylene-glycol-3350-in-occasional-constipation-a-one-week-randomized-placebo-controlled-double-blind-trial
#15
Thomas McGraw
AIM: To evaluate the efficacy and safety of polyethylene glycol (PEG) 3350 in subjects with self-reported occasional constipation. METHODS: Eligible subjects ≥ 17 years of age were randomized to receive either placebo or PEG 3350 17 g once daily in this multicenter, double-blind trial. Evaluations were conducted before (baseline) and after a 7-d treatment period. The primary efficacy variable was the proportion of subjects reporting complete resolution of straining and hard or lumpy stools...
May 6, 2016: World Journal of Gastrointestinal Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/27109994/optimal-solution-volume-for-luminal-preservation-a-preclinical-study-in-porcine-intestinal-preservation
#16
M Oltean, M Papurica, L Jiga, B Hoinoiu, C Glameanu, A Bresler, G Patrut, R Grigorie, M Ionac, M Hellström
BACKGROUND: Rodent studies suggest that luminal solutions alleviate the mucosal injury and prolong intestinal preservation but concerns exist that excessive volumes of luminal fluid may promote tissue edema. Differences in size, structure, and metabolism between rats and humans require studies in large animals before clinical use. METHODS: Intestinal procurement was performed in 7 pigs. After perfusion with histidine-tryptophan-ketoglutarate (HTK), 40-cm-long segments were cut and filled with 13...
March 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27041744/drop-printing-of-pharmaceuticals-effect-of-molecular-weight-on-peg-coated-naproxen-peg3350-solid-dispersions
#17
Hsin-Yun Hsu, Scott Toth, Garth J Simpson, Michael T Harris
Solid dispersions have been used to enhance the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs). However, the solid state phase, compositional uniformity, and scale-up problems are issues that need to be addressed. To allow for highly controllable products, the Drop Printing (DP) technique can provide precise dosages and predictable compositional uniformity of APIs in two/three dimensional structures. In this study, DP was used to prepare naproxen (NAP)/polyethylene glycol 3350 (PEG3350) solid dispersions with PEG coatings of different molecular weights (MW)...
December 2015: AIChE Journal
https://www.readbyqxmd.com/read/27022668/aqueous-two-phase-system-purification-for-superoxide-dismutase-induced-by-menadione-from-phanerochaete-chrysosporium
#18
Berna Kavakcıoğlu, Burcu Tongul, Leman Tarhan
In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K2HPO4 5% (w/w) with pH 7...
March 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/26989345/additive-effect-on-soybean-peroxidase-catalyzed-removal-of-anilines-from-water
#19
Samar Mazloum, Mohammed Mousa Al-Ansari, Keith Taylor, Jatinder K Bewtra, Niharendu Biswas
Soybean peroxidase has been shown to be effective in removal of aromatic compounds from wastewater, while the use of additives effectively reduces enzyme concentration requirement, hence overall treatment cost. Enzymatic treatment, an oxidative polymerization, was successful in removal of over 95% of both aniline and o-anisidine. The originality of this study lies in the findings that the additives, sodium dodecyl sulfate (SDS), sodium dodecylbenzenesulfonate (SDBS), Triton X-100, and sodium dodecanoate (SDOD), reduced enzyme concentration requirement, while polyethylene glycol (PEG, average molar mass of 3350 g/mol) had no effect on the required enzyme concentration...
February 1, 2016: Environmental Engineering Science
https://www.readbyqxmd.com/read/26945825/polyethylene-glycol-plus-ascorbic-acid-is-as-effective-as-sodium-picosulfate-with-magnesium-citrate-for-bowel-preparation-a-randomized-trial
#20
RANDOMIZED CONTROLLED TRIAL
Hyun-Seok Choi, Jun-Won Chung, Ji Won Lee, Min Young Lim, Dong Kyun Park, Yoon Jae Kim, Kwang Ahn Kwon, Jung Ho Kim
OBJECTIVE: This study was aimed to evaluate the efficacy and safety of two low-volume agents, polyethylene glycol (PEG)-3350 plus ascorbic acid (PEG + Asc) and sodium picosulfate with magnesium citrate (SPMC), for bowel preparation. METHODS: We performed a prospective, endoscopist-blinded, single-center, randomized controlled trial comparing PEG + Asc with SPMC to evaluate the bowel cleansing efficacy of the two regimens using the modified Ottawa bowel preparation scale (OBPS) and the Aronchick scale...
April 2016: Journal of Digestive Diseases
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