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peg 3350

Naseer Iqbal, Mukesh Kumar, Pradeep Sharma, Satya Prakash Yadav, Punit Kaur, Sujata Sharma, Tej P Singh
Dehydroquinase (3-dehydroquinate dehydratase, DHQD, EC catalyzes the conversion of dehydroquinate to dehydroshikimate. DHQD from Acinetobacter baumannii (AbDHQD) was cloned, expressed and purified to homogeneity. The binding studies showed that two compounds quinic acid and citrazinic acid bound to AbDHQD at micromolar concentrations. AbDHQD was crystallized using 30% PEG-3350, 50mM tris-HCl and 1.0M MgSO4 at PH 8.0. Crystals of AbDHQD were stabilized with 25% glycerol for data collection at 100K...
October 18, 2016: International Journal of Biological Macromolecules
Swapnil Sharadkumar Chudiwal, Mohamed Hassan G Dehghan
The objective of the present study was to design and develop drug-device combination product in particular flunisolide nasal spray using quality by design (QbD) approach. Quality target product profile (QTPP) of flunisolide nasal spray was defined and critical quality attributes (CQAs) i.e. viscosity (cp) (Y1) and D50 DSD (µm) (Y2) were identified. Potential risk factors were identified using a fish bone diagram and failure mode effect analysis (FMEA) tools. Plackett Burman and Box Behnken design were used for screening the significant factors and optimizing the variables range respectively...
September 10, 2016: Pharmaceutical Development and Technology
Brian J Carlisle, Garrett Craft, Julie P Harmon, Alina Ilkevitch, Jenik Nicoghosian, Inna Sheyner, Jonathan T Stewart
Clinicians commonly encounter dysphagia and constipation in a skilled nursing population. Increasing the viscosity of liquids, usually with a starch- or xanthan gum-based thickener, serves as a key intervention for patients with dysphagia. We report a newly identified and potentially dangerous interaction between polyethylene glycol 3350 laxative (PEG) and starch-thickened liquids. A patient requiring nectar-thickened liquids became constipated, and medical staff prescribed PEG for constipation. His nurse observed that the thickened apple juice immediately thinned to near-water consistency when PEG was added...
September 1, 2016: Journal of the American Medical Directors Association
So Hee Lee, Sun Hyuk Hwang, Jin Soo Park, Hae Sim Park, Yoo Seob Shin
Polyethylene glycols (PEGs) are believed to be chemically inert agents, but larger PEG polymers could have immunogenicity. A 39-year-old man was referred to emergency room for loss of consciousness and dyspnea after taking of PEG-3350 (Colyte®). In laboratory findings, the initial serum tryptase level was increased to 91.9 mg/L (normal range: 0.00-11.40 mg/L) without any other laboratory abnormalities. The intradermal test with 10 mg/mL Colyte® showed a 5 × 5 mm wheal, but basophil activation and histamine releasability tests were negative...
October 2016: Journal of Korean Medical Science
Thomas McGraw
PURPOSE: To evaluate the safety and tolerability of aqueous solution concentrate (ASC) of polyethylene glycol (PEG) 3350 in patients with functional constipation. PATIENTS AND METHODS: The patients who met Rome III diagnostic criteria for functional constipation were randomized in this multicenter, randomized, placebo-controlled, single-blind study to receive once daily dose of PEG 3350 (17 g) ASC or placebo solution for 14 days. The study comprised a screening period (visit 1), endoscopy procedure (visits 2 and 3), and followup telephone calls 30 days post-treatment...
2016: Clinical and Experimental Gastroenterology
Isabel Miquel, María Eugenia Arancibia, Francisco Alliende, Gloria Ríos, Lorena Rodríguez, Yalda Lucero, Eric Saelzer
INTRODUCTION: Adequate intestinal cleanliness is crucial to achieve optimal colonoscopy performance. Several bowel preparation (BP) schemes have been proposed, but there is still no consensus as regards which is the most suitable in paediatric patients. OBJECTIVE: To describe the effectiveness, adherence, and adverse effects of BP protocols differentiated by age group in paediatric patients subjected to colonoscopy. PATIENTS AND METHOD: Prospective, study that included patients <18 years subjected to colonoscopy...
July 15, 2016: Revista Chilena de Pediatría
Naheda Sahtout, Jijin R A Kuttiyatveetil, Michel Fodje, David A R Sanders
Thioredoxin is a small ubiquitous protein that plays a role in many biological processes. A putative thioredoxin, Trx1, from Thermosipho africanus strain TCF52B, which has low sequence identity to its closest homologues, was successfully cloned, overexpressed and purified. The protein was crystallized using the microbatch-under-oil technique at 289 K in a variety of conditions; crystals grown in 0.2 M MgCl2, 0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350, which grew as irregular trapezoids to maximum dimensions of 1...
June 2016: Acta Crystallographica. Section F, Structural Biology Communications
Michael Hodgman, Michael G Holland, Ulrich Englich, Susan M Wojcik, William D Grant, Erich Leitner
INTRODUCTION: Whole bowel irrigation (WBI) is a management option for overdose of medications poorly adsorbed to activated charcoal, with modified release properties, or for body packers. Polyethylene glycol (PEG) is a mixture of ethylene oxide polymers of varying molecular weight. PEG with an average molecular weight of 3350 g/mol is used for WBI. PEG electrolyte lavage solution has been shown in vitro to hasten the dissolution of acetaminophen. The impact of PEG on the pharmacokinetics of extended release pharmaceuticals is unknown...
June 13, 2016: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
Thomas McGraw
AIM: To evaluate the efficacy and safety of polyethylene glycol (PEG) 3350 in subjects with self-reported occasional constipation. METHODS: Eligible subjects ≥ 17 years of age were randomized to receive either placebo or PEG 3350 17 g once daily in this multicenter, double-blind trial. Evaluations were conducted before (baseline) and after a 7-d treatment period. The primary efficacy variable was the proportion of subjects reporting complete resolution of straining and hard or lumpy stools...
May 6, 2016: World Journal of Gastrointestinal Pharmacology and Therapeutics
M Oltean, M Papurica, L Jiga, B Hoinoiu, C Glameanu, A Bresler, G Patrut, R Grigorie, M Ionac, M Hellström
BACKGROUND: Rodent studies suggest that luminal solutions alleviate the mucosal injury and prolong intestinal preservation but concerns exist that excessive volumes of luminal fluid may promote tissue edema. Differences in size, structure, and metabolism between rats and humans require studies in large animals before clinical use. METHODS: Intestinal procurement was performed in 7 pigs. After perfusion with histidine-tryptophan-ketoglutarate (HTK), 40-cm-long segments were cut and filled with 13...
March 2016: Transplantation Proceedings
Hsin-Yun Hsu, Scott Toth, Garth J Simpson, Michael T Harris
Solid dispersions have been used to enhance the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs). However, the solid state phase, compositional uniformity, and scale-up problems are issues that need to be addressed. To allow for highly controllable products, the Drop Printing (DP) technique can provide precise dosages and predictable compositional uniformity of APIs in two/three dimensional structures. In this study, DP was used to prepare naproxen (NAP)/polyethylene glycol 3350 (PEG3350) solid dispersions with PEG coatings of different molecular weights (MW)...
December 2015: AIChE Journal
Berna Kavakcıoğlu, Burcu Tongul, Leman Tarhan
In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC, an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K2HPO4 5% (w/w) with pH 7...
March 29, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Samar Mazloum, Mohammed Mousa Al-Ansari, Keith Taylor, Jatinder K Bewtra, Niharendu Biswas
Soybean peroxidase has been shown to be effective in removal of aromatic compounds from wastewater, while the use of additives effectively reduces enzyme concentration requirement, hence overall treatment cost. Enzymatic treatment, an oxidative polymerization, was successful in removal of over 95% of both aniline and o-anisidine. The originality of this study lies in the findings that the additives, sodium dodecyl sulfate (SDS), sodium dodecylbenzenesulfonate (SDBS), Triton X-100, and sodium dodecanoate (SDOD), reduced enzyme concentration requirement, while polyethylene glycol (PEG, average molar mass of 3350 g/mol) had no effect on the required enzyme concentration...
February 1, 2016: Environmental Engineering Science
Hyun-Seok Choi, Jun-Won Chung, Ji Won Lee, Min Young Lim, Dong Kyun Park, Yoon Jae Kim, Kwang Ahn Kwon, Jung Ho Kim
OBJECTIVE: This study was aimed to evaluate the efficacy and safety of two low-volume agents, polyethylene glycol (PEG)-3350 plus ascorbic acid (PEG + Asc) and sodium picosulfate with magnesium citrate (SPMC), for bowel preparation. METHODS: We performed a prospective, endoscopist-blinded, single-center, randomized controlled trial comparing PEG + Asc with SPMC to evaluate the bowel cleansing efficacy of the two regimens using the modified Ottawa bowel preparation scale (OBPS) and the Aronchick scale...
April 2016: Journal of Digestive Diseases
Siti Nurulnabila A Rahaman, Jastina Mat Yusop, Zeti-Azura Mohamed-Hussein, Kok Lian Ho, Aik-Hong Teh, Jitka Waterman, Chyan Leong Ng
C1ORF123 is a human hypothetical protein found in open reading frame 123 of chromosome 1. The protein belongs to the DUF866 protein family comprising eukaryote-conserved proteins with unknown function. Recent proteomic and bioinformatic analyses identified the presence of C1ORF123 in brain, frontal cortex and synapses, as well as its involvement in endocrine function and polycystic ovary syndrome (PCOS), indicating the importance of its biological role. In order to provide a better understanding of the biological function of the human C1ORF123 protein, the characterization and analysis of recombinant C1ORF123 (rC1ORF123), including overexpression and purification, verification by mass spectrometry and a Western blot using anti-C1ORF123 antibodies, crystallization and X-ray diffraction analysis of the protein crystals, are reported here...
March 2016: Acta Crystallographica. Section F, Structural Biology Communications
Curie Javiya, Sriramakamal Jonnalagadda
CONTEXT: The use of spray-drying to prepare blended PLGA:PEG microspheres with lower immune detection. OBJECTIVE: To study physical properties, polymer miscibility and alveolar macrophage response for blended PLGA:PEG microspheres prepared by a laboratory-scale spray-drying process. METHODS: Microspheres were prepared by spray-drying 0-20% w/w ratios of PLGA 65:35 and PEG 3350 in dichloromethane. Particle size and morphology was studied using scanning electron microscopy...
September 2016: Drug Development and Industrial Pharmacy
Edith Espitia-Saloma, Patricia Vâzquez-Villegas, Marco Rito-Palomares, Oscar Aguilar
Aqueous two-phase systems (ATPS) are a liquid-liquid extraction technology with clear process benefits; however, its lack of industrial embracement is still a challenge to overcome. Antibodies are a potential product to be recovered by ATPS in a commercial context. The objective of this work is to present a more integral approach of the different isolated strategies that have arisen in order to enable a practical, generic implementation of ATPS, using human immunoglobulin G (IgG) as experimental model. A microfluidic device is used for ATPS parameters preselection for product recovery...
May 2016: Biotechnology Journal
Sultan M Alshehri, Roshan V Tiwari, Bader B Alsulays, Eman A Ashour, Abdullah S Alshetaili, Bjad Almutairy, Jun-Bom Park, Joseph Morott, Bhupinder Sandhu, Soumyajit Majumdar, Michael A Repka
This study aimed to investigate the combined effect of magnesium oxide (MgO) as an alkalizer and polyethylene glycol (PEG) as a plasticizer and wetting agent in the presence of Kollidon® 12 PF and 17 PF polymer carriers on the release profile of mefenamic acid (MA), which was prepared via hot-melt extrusion technique. Various drug loads of MA and various ratios of the polymers, PEG 3350 and MgO were blended using a V-shell blender and extruded using a twin-screw extruder (16-mm Prism EuroLab, ThermoFisher Scientific, Carlsbad, CA) at different screw speeds and temperatures to prepare a solid dispersion system...
January 29, 2016: Pharmaceutical Development and Technology
Leslie N Rhodes, Deborah G Loman, Margaret W Bultas
BACKGROUND: Orthopaedic procedures place children at risk for postoperative constipation due to combined effects of anesthesia, narcotics, and decreased physical mobility. PURPOSE: This retrospective study analyzed medication use and stool outcomes of 36 children who received polyethylene glycol 3350 (PEG) or mineral oil (MO) after a spinal fusion. METHODS AND RESULTS: A chart review found no statistical differences by group for number of bowel movements (BMs) before discharge (p = ...
January 2016: Orthopaedic Nursing
Michele R Spier, Francieli B Siepmann, Larissa Staack, Priscila Z Souza, Vikas Kumar, Adriane B P Medeiros, Carlos R Soccol
The development of stable enzymes is a key issue in both the food and feed industries. Consequently, the aim of the current study is to evaluate the impact of various additives (sodium chloride, sodium citrate, mannitol, methylparaben, polyethylene glycol 3350, ethylenediaminetetraacetic acid disodium salt, and a serine protease inhibitor) on the stability of a mushroom phytase produced by solid-state cultivation and recovery. Also observed was the effect of the additives on microbial growth inhibition by monitoring both the change in optical density over 30 days of storage and proteolytic activity...
October 2, 2016: Preparative Biochemistry & Biotechnology
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