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https://www.readbyqxmd.com/read/29095157/pteridine-glycosyltransferase-from-chlorobium-tepidum-crystallization-and-x-ray-analysis
#1
Asaithambi Killivalavan, Young Shik Park, Kon Ho Lee
The pteridine glycosyltransferase (PGT) found in Chlorobium tepidum (CtPGT) catalyzes the conversion of L-threo-tetrahydrobiopterin to 1-O-(L-threo-biopterin-2'-yl)-β-N-acetylglucosamine using UDP-N-acetylglucosamine. The gene for CtPGT was cloned, and selenomethionine-derivatized protein was overexpressed and purified using various chromatographic techniques. The protein was crystallized by the hanging-drop vapour-diffusion method using 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350 as a reservoir solution...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29095154/crystallization-and-x-ray-diffraction-analysis-of-native-and-selenomethionine-substituted-phyh-di-from-bacillus-sp-hjb17
#2
Fang Lu, Bei Zhang, Yong Liu, Ying Song, Gangxing Guo, Duo Feng, Huoqing Huang, Peilong Yang, Wei Gao, Sujuan Guo, Bin Yao
Phytases are phosphatases that hydrolyze phytates to less phosphorylated myo-inositol derivatives and inorganic phosphate. β-Propeller phytases, which are very diverse phytases with improved thermostability that are active at neutral and alkaline pH and have absolute substrate specificity, are ideal substitutes for other commercial phytases. PhyH-DI, a β-propeller phytase from Bacillus sp. HJB17, was found to act synergistically with other single-domain phytases and can increase their efficiency in the hydrolysis of phytate...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29050764/pharmacokinetic-studies-of-a-three-component-complex-that-repurposes-the-front-line-antibiotic-isoniazid-against-mycobacterium-tuberculosis
#3
Thomas J Manning, Kyle Wilkerson, Taylor Holder, Andrew Carson Bartley, Chelsea Jackson, Sydney Plummer, Dennis Phillips, Logan Krajewski, Greg Wylie
The frontline tuberculosis (Tb) antibiotic isoniazid has been repurposed using a three component complex aimed at increasing the delivery efficiency and adding new avenues to its mechanism of action. This study focuses on pharmacokinetic studies of the isoniazid-sucrose-copper (II)-PEG-3350 complex. The assays include the Plasma Protein Binding Assay (85.8%), Caco-2 Permeability Assay (B→APapp, 0.13 × 10(-6) cm/s), Cytochrome P450 Inhibition Assay (i.e. CYP2B6, IC50 = 7.26 μM), In vitro microsomal Stability Assay (t1/2 NADPH-Dependent > 240 min), and HepG2 Cytotoxicity (no toxicity)...
December 2017: Tuberculosis
https://www.readbyqxmd.com/read/28906317/polyethylene-glycol-3350-with-electrolytes-vs-polyethylene-glycol-4000-for-constipation-a-randomized-controlled-trial
#4
Noor L H Bekkali, Daniël R Hoekman, Olivia Liem, Marloes E J Bongers, Michiel P van Wijk, Bas Zegers, Rolf A Pelleboer, Wim Verwijs, Bart G P Koot, Maksym Voropaiev, Marc A Benninga
OBJECTIVES: The long-term efficacy and safety of polyethylene glycol (PEG) in constipated children are unknown, and a head-to-head comparison of the different PEG formulations is lacking. We aimed to investigate non-inferiority of PEG3350 with electrolytes (PEG3350+E) compared to PEG4000 without electrolytes (PEG4000). METHODS: In this double-blind trial, children aged 0.5 - 16 years with constipation, defined as a defecation frequency of < 3 times per week, were randomized to receive either PEG3350 + E or PEG4000...
September 12, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28807549/a-multiplexed-microfluidic-toolbox-for-the-rapid-optimization-of-affinity-driven-partition-in-aqueous-two-phase-systems
#5
Eduardo J S Bras, Ruben R G Soares, Ana M Azevedo, Pedro Fernandes, Miguel Arévalo-Rodríguez, Virginia Chu, João P Conde, M Raquel Aires-Barros
Antibodies and other protein products such as interferons and cytokines are biopharmaceuticals of critical importance which, in order to be safely administered, have to be thoroughly purified in a cost effective and efficient manner. The use of aqueous two-phase extraction (ATPE) is a viable option for this purification, but these systems are difficult to model and optimization procedures require lengthy and expensive screening processes. Here, a methodology for the rapid screening of antibody extraction conditions using a microfluidic channel-based toolbox is presented...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28777087/methylation-crystallization-and-sad-phasing-of-the-csu-pilus-csuc-csue-chaperone-adhesin-subunit-pre-assembly-complex-from-acinetobacter-baumannii
#6
Natalia Pakharukova, Minna Tuittila, Sari Paavilainen, Anton Zavialov
Acinetobacter baumannii is one of the most difficult Gram-negative bacteria to control and treat. This pathogen forms biofilms on hospital surfaces and medical devices using Csu pili assembled via the archaic chaperone-usher pathway. To uncover the mechanism of bacterial attachment to abiotic surfaces, it was aimed to determine the crystal structure of the pilus tip adhesin CsuE. The CsuC-CsuE chaperone-subunit pre-assembly complex was purified from the periplasm of Escherichia coli overexpressing CsuC and CsuE...
August 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28580919/crystallization-and-x-ray-diffraction-analysis-of-spae-a-basal-pilus-protein-from-the-gut-adapted-lactobacillus-rhamnosus-gg
#7
Arjun K Mishra, Abhin Kumar Megta, Airi Palva, Ingemar von Ossowski, Vengadesan Krishnan
SpaE is the predicted basal pilin subunit in the sortase-dependent SpaFED pilus from the gut-adapted and commensal Lactobacillus rhamnosus GG. Thus far, structural characterization of the cell-wall-anchoring basal pilins has remained difficult and has been limited to only a few examples from pathogenic genera and species. To gain a further structural understanding of the molecular mechanisms that are involved in the anchoring and assembly of sortase-dependent pili in less harmful bacteria, L. rhamnosus GG SpaE for crystallization was produced by recombinant expression in Escherichia coli...
June 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28522396/ultrafast-spectroscopy-on-dna-cleavage-by-endonuclease-in-molecular-crowding
#8
Priya Singh, Susobhan Choudhury, Shreyasi Dutta, Aniruddha Adhikari, Siddhartha Bhattacharya, Debasish Pal, Samir Kumar Pal
The jam-packed intracellular environments differ the activity of a biological macromolecule from that in laboratory environments (in vitro) through a number of mechanisms called molecular crowding related to structure, function and dynamics of the macromolecule. Here, we have explored the structure, function and dynamics of a model enzyme protein DNase I in molecular crowing of polyethylene glycol (PEG; MW 3350). We have used steady state and picosecond resolved dynamics of a well-known intercalator ethidium bromide (EB) in a 20-mer double-stranded DNA (dsDNA) to monitor the DNA-cleavage by the enzyme in absence and presence PEG...
October 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28382323/the-impact-of-diet-liberalization-on-bowel-preparation-for-colonoscopy
#9
James Walter, Gloria Francis, Rebecca Matro, Ramalinga Kedika, Rachael Grosso, Scott W Keith, David Kastenberg
Background and study aims Dietary restrictions are integral to colonoscopy preparation and impact patient satisfaction. Utilizing split-dose, lower-volume polyethylene glycol 3350-electrolyte solution (PEG-ELS), this study compared colon preparation adequacy of a low-residue diet to clear liquids using a validated grading scale. Patients and methods This was a prospective, randomized, single-blinded, single-center non-inferiority study evaluating diet the day prior to outpatient colonoscopy. Subjects were randomized to a Low-Residue diet for breakfast and lunch, or Clears only...
April 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28242427/purification-and-characterization-of-a-collagenase-from-penicillium-sp-ucp-1286-by-polyethylene-glycol-phosphate-aqueous-two-phase-system
#10
Maria Carolina de Albuquerque Wanderley, José Manoel Wanderley Duarte Neto, Wendell Wagner Campos Albuquerque, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima, Sara Isabel da Cruz Silvério, José Luiz de Lima Filho, José António Couto Teixeira, Ana Lúcia Figueiredo Porto
Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including low-processing time, low-cost material and low-energy consumption. The collagenase produced by Penicillium sp...
May 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28147222/seventy-years-of-polyethylene-glycols-in-gastroenterology-the-journey-of-peg-4000-and-3350-from-nonabsorbable-marker-to-colonoscopy-preparation-to-osmotic-laxative
#11
EDITORIAL
John S Fordtran, Alan F Hofmann
No abstract text is available yet for this article.
March 2017: Gastroenterology
https://www.readbyqxmd.com/read/28110184/structural-analysis-of-binding-functionality-of-folic-acid-peg-dendrimers-against-folate-receptor
#12
Diana Sampogna-Mireles, Ingrid D Araya-Durán, Valeria Márquez-Miranda, Jesús A Valencia-Gallegos, Fernando D González-Nilo
Dendrimers functionalized with folic acid (FA) are drug delivery systems that can selectively target cancer cells with folate receptors (FR-α) overexpression. Incorporation of polyethylene glycol (PEG) can enhance dendrimers solubility and pharmacokinetics, but ligand-receptor binding must not be affected. In this work we characterized, at atomic level, the binding functionality of conventional site-specific dendrimers conjugated with FA with PEG 750 or PEG 3350 as a linker. After Molecular Dynamics simulation, we observed that both PEG's did not interfere over ligand-receptor binding functionality...
March 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/27769928/binding-studies-and-structure-determination-of-the-recombinantly-produced-type-ii-3-dehydroquinate-dehydratase-from-acinetobacter-baumannii
#13
Naseer Iqbal, Mukesh Kumar, Pradeep Sharma, Satya Prakash Yadav, Punit Kaur, Sujata Sharma, Tej P Singh
Dehydroquinase (3-dehydroquinate dehydratase, DHQD, EC 4.2.1.10) catalyzes the conversion of dehydroquinate to dehydroshikimate. DHQD from Acinetobacter baumannii (AbDHQD) was cloned, expressed and purified to homogeneity. The binding studies showed that two compounds quinic acid and citrazinic acid bound to AbDHQD at micromolar concentrations. AbDHQD was crystallized using 30% PEG-3350, 50mM tris-HCl and 1.0M MgSO4 at pH 8.0. Crystals of AbDHQD were stabilized with 25% glycerol for data collection at 100K...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27616074/quality-by-design-qbd-approach-for-design-and-development-of-drug-device-combination-products-a-case-study-on-flunisolide-nasal-spray
#14
Swapnil Sharadkumar Chudiwal, Mohamed Hassan G Dehghan
The objective of the present study was to design and develop drug-device combination product in particular flunisolide nasal spray using quality by design (QbD) approach. Quality target product profile (QTPP) of flunisolide nasal spray was defined and critical quality attributes (CQAs) i.e. viscosity (cp) (Y1) and D50 DSD (µm) (Y2) were identified. Potential risk factors were identified using a fish bone diagram and failure mode effect analysis (FMEA) tools. Plackett Burman and Box Behnken design were used for screening the significant factors and optimizing the variables range respectively...
September 10, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27569713/peg-and-thickeners-a-critical-interaction-between-polyethylene-glycol-laxative-and-starch-based-thickeners
#15
Brian J Carlisle, Garrett Craft, Julie P Harmon, Alina Ilkevitch, Jenik Nicoghosian, Inna Sheyner, Jonathan T Stewart
Clinicians commonly encounter dysphagia and constipation in a skilled nursing population. Increasing the viscosity of liquids, usually with a starch- or xanthan gum-based thickener, serves as a key intervention for patients with dysphagia. We report a newly identified and potentially dangerous interaction between polyethylene glycol 3350 laxative (PEG) and starch-thickened liquids. A patient requiring nectar-thickened liquids became constipated, and medical staff prescribed PEG for constipation. His nurse observed that the thickened apple juice immediately thinned to near-water consistency when PEG was added...
September 1, 2016: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/27550498/anaphylaxis-to-polyethylene-glycol-colyte%C3%A2-in-a-patient-with-diverticulitis
#16
So Hee Lee, Sun Hyuk Hwang, Jin Soo Park, Hae Sim Park, Yoo Seob Shin
Polyethylene glycols (PEGs) are believed to be chemically inert agents, but larger PEG polymers could have immunogenicity. A 39-year-old man was referred to emergency room for loss of consciousness and dyspnea after taking of PEG-3350 (Colyte®). In laboratory findings, the initial serum tryptase level was increased to 91.9 mg/L (normal range: 0.00-11.40 mg/L) without any other laboratory abnormalities. The intradermal test with 10 mg/mL Colyte® showed a 5 × 5 mm wheal, but basophil activation and histamine releasability tests were negative...
October 2016: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/27486340/safety-of-polyethylene-glycol-3350-solution-in-chronic-constipation-randomized-placebo-controlled-trial
#17
Thomas McGraw
PURPOSE: To evaluate the safety and tolerability of aqueous solution concentrate (ASC) of polyethylene glycol (PEG) 3350 in patients with functional constipation. PATIENTS AND METHODS: The patients who met Rome III diagnostic criteria for functional constipation were randomized in this multicenter, randomized, placebo-controlled, single-blind study to receive once daily dose of PEG 3350 (17 g) ASC or placebo solution for 14 days. The study comprised a screening period (visit 1), endoscopy procedure (visits 2 and 3), and followup telephone calls 30 days post-treatment...
2016: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/27425772/-intestinal-cleaning-for-colonoscopy-in-children-effectiveness-adherence-and-adverse-effects-of-schemes-differentiated-by-age
#18
Isabel Miquel, María Eugenia Arancibia, Francisco Alliende, Gloria Ríos, Lorena Rodríguez, Yalda Lucero, Eric Saelzer
Adequate intestinal cleanliness is crucial to achieve optimal colonoscopy performance. Several bowel preparation (BP) schemes have been proposed, but there is still no consensus as regards which is the most suitable in paediatric patients. OBJECTIVE: To describe the effectiveness, adherence, and adverse effects of BP protocols differentiated by age group in paediatric patients subjected to colonoscopy. PATIENTS AND METHOD: Prospective, study that included patients < 18 years subjected to colonoscopy...
April 2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/27303896/putative-thioredoxin-trx1-from-thermosipho-africanus-strain-tcf52b-expression-purification-and-structural-determination-using-s-sad
#19
Naheda Sahtout, Jijin R A Kuttiyatveetil, Michel Fodje, David A R Sanders
Thioredoxin is a small ubiquitous protein that plays a role in many biological processes. A putative thioredoxin, Trx1, from Thermosipho africanus strain TCF52B, which has low sequence identity to its closest homologues, was successfully cloned, overexpressed and purified. The protein was crystallized using the microbatch-under-oil technique at 289 K in a variety of conditions; crystals grown in 0.2 M MgCl2, 0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350, which grew as irregular trapezoids to maximum dimensions of 1...
June 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27295188/the-influence-of-polyethylene-glycol-solution-on-the-dissolution-rate-of-sustained-release-morphine
#20
Michael Hodgman, Michael G Holland, Ulrich Englich, Susan M Wojcik, William D Grant, Erich Leitner
INTRODUCTION: Whole bowel irrigation (WBI) is a management option for overdose of medications poorly adsorbed to activated charcoal, with modified release properties, or for body packers. Polyethylene glycol (PEG) is a mixture of ethylene oxide polymers of varying molecular weight. PEG with an average molecular weight of 3350 g/mol is used for WBI. PEG electrolyte lavage solution has been shown in vitro to hasten the dissolution of acetaminophen. The impact of PEG on the pharmacokinetics of extended release pharmaceuticals is unknown...
December 2016: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
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