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https://www.readbyqxmd.com/read/28079053/molecular-evidence-for-the-involvement-of-a-polygalacturonase-inhibiting-protein-ghpgip1-in-enhanced-resistance-to-verticillium-and-fusarium-wilts-in-cotton
#1
Nana Liu, Xueyan Zhang, Yun Sun, Ping Wang, Xiancai Li, Yakun Pei, Fuguang Li, Yuxia Hou
Polygalacturonase-inhibiting protein (PGIP), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. Herein, we showed that purified GhPGIP1 is a functional inhibitor of Verticillium dahliae and Fusarium oxysporum f. sp. vasinfectum, the two fungal pathogens causing cotton wilt. Transcription of GhPGIP1 was increased in cotton upon infection, wounding, and treatment with defence hormone and H2O2. Resistance by GhPGIP1 was examined by its virus-induced gene silencing in cotton and overexpression in Arabidopsis...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069498/poly-glycerol-sebacate-poly-%C3%AE%C2%B5-caprolactone-blend-nanofibrous-scaffold-as-intrinsic-bio-and-immunocompatible-system-for-corneal-repair
#2
Sahar Salehi, Marta Czugala, Piotr Stafiej, Mohammadhossein Fathi, Thomas Bahners, Jochen S Gutmann, Bernhard B Singer, Thomas A Fuchsluger
: A major challenge in corneal tissue engineering and lamellar corneal transplantation is to develop synthetic scaffolds able to simulate the optical and mechanical properties of the native cornea. As a carrier, the graft scaffolds should provide the basis for anchorage, repair and regeneration. Although quite a number of scaffolds have been engineered to date, they have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the corneal extracellular matrix (ECM)...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#3
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28050963/live-births-after-polar-body-biopsy-and-frozen-thawed-cleavage-stage-embryo-transfer-case-report
#4
Fernando Guimarães, Matheus Roque, Marcello Valle, Alessandra Kostolias, Rodrigo A de Azevedo, Ciro D Martinhago, Marcos Sampaio, Selmo Geber
Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28047972/tu-fg-brb-05-a-3-dimensional-prompt-gamma-imaging-system-for-range-verification-in-proton-radiotherapy
#5
E Draeger, H Chen, D Mackin, S Peterson, S Avery, S Beddar, J Polf
PURPOSE: To report on the initial developments of a clinical 3-dimensional (3D) prompt gamma (PG) imaging system for proton radiotherapy range verification. METHODS: The new imaging system under development consists of a prototype Compton camera to measure PG emission during proton beam irradiation and software to reconstruct, display, and analyze 3D images of the PG emission. For initial test of the system, PGs were measured with a prototype CC during a 200 cGy dose delivery with clinical proton pencil beams (ranging from 100 MeV - 200 MeV) to a water phantom...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28043888/uterine-and-placental-expression-of-hpgd-in-cows-during-pregnancy-and-release-of-fetal-membranes
#6
Jessica von Hof, Nele Sprekeler, Gerhard Schuler, Alois Boos, Mariusz P Kowalewski
15-hydroxyprostaglandin dehydrogenase (HPGD) plays a key role in prostaglandins (PGs) catabolism. Its expression and activity appear to be regulated by progesterone (P4). We investigated the HPGD mRNA-expression and protein localization in placentomes and interplacental uterine sites throughout gestation (Study I), and after fetal membranes retention (RFM) compared with normally delivered fetal membranes (DFM) (Study II). Furthermore, we analyzed the influence of aglepristone (AP), dexamethasone (GC) or cloprostenol (CP), on HPGD expression in bovine placentomes (Study III)...
December 30, 2016: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/28042832/r-flurbiprofen-traps-prostaglandins-within-cells-by-inhibition-of-multidrug-resistance-associated-protein-4
#7
Ivonne Wobst, Lisa Ebert, Kerstin Birod, Marthe-Susanna Wegner, Marika Hoffmann, Dominique Thomas, Carlo Angioni, Michael J Parnham, Dieter Steinhilber, Irmgard Tegeder, Gerd Geisslinger, Sabine Grösch
R-flurbiprofen is the non-COX-inhibiting enantiomer of flurbiprofen and is not converted to S-flurbiprofen in human cells. Nevertheless, it reduces extracellular prostaglandin E₂ (PGE₂) in cancer or immune cell cultures and human extracellular fluid. Here, we show that R-flurbiprofen acts through a dual mechanism: (i) it inhibits the translocation of cPLA2α to the plasma membrane and thereby curtails the availability of arachidonic acid and (ii) R-flurbiprofen traps PGE₂ inside of the cells by inhibiting multidrug resistance-associated protein 4 (MRP4, ABCC4), which acts as an outward transporter for prostaglandins...
December 30, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28042415/chances-to-have-a-boy-after-gender-selection-by-pre-implantation-genetic-screening-are-reduced-in-couples-with-only-girls-and-without-a-boy-sired-by-the-male-partner
#8
Soryya Panahi, Fariba Fahami, Mohammad Reza Deemeh, Marziyeh Tavalaee, Hamid Gourabi, Mohammad Hossain Nasr-Esfahani
BACKGROUND: Gender selection and family planning have their roots in human history. Despite great interest in these fields, very few scientific propositions exist which could explain why some family do not attain the desired sex. Therefore, the aim of this study was to evaluate whether sex of previous child or children could affect the outcomes of pre-implantation genetic screening (PGS). MATERIALS AND METHODS: This historical cohort study including 218 PGS cases referring to Isfahan Fertility and Infertility Center (IFIC)...
January 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28025520/plastoglobule-targeting-competence-of-a-putative-transit-peptide-sequence-from-rice-phytoene-synthase-2-in-plastids
#9
Min Kyoung You, Jin Hwa Kim, Yeo Jin Lee, Ye Sol Jeong, Sun-Hwa Ha
Plastoglobules (PGs) are thylakoid membrane microdomains within plastids that are known as specialized locations of carotenogenesis. Three rice phytoene synthase proteins (OsPSYs) involved in carotenoid biosynthesis have been identified. Here, the N-terminal 80-amino-acid portion of OsPSY2 (PTp) was demonstrated to be a chloroplast-targeting peptide by displaying cytosolic localization of OsPSY2(ΔPTp):mCherry in rice protoplast, in contrast to chloroplast localization of OsPSY2:mCherry in a punctate pattern...
December 22, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28024592/bi-layered-constructs-of-poly-glycerol-sebacate-%C3%AE-tricalcium-phosphate-for-bone-soft-tissue-interface-applications
#10
Atakan Tevlek, Pezhman Hosseinian, Cansel Ogutcu, Mustafa Turk, Halil Murat Aydin
This study aims to establish a facile protocol for the preparation of a bi-layered poly(glycerol-sebacate) (PGS)/β-tricalcium phosphate (β-TCP) construct and to investigate its potential for bone-soft tissue engineering applications. The layered structure was prepared by distributing the ceramic particles within a prepolymer synthesized in a microwave reactor followed by a cross-linking of the final construct in vacuum (<10mbar). The vacuum stage led to the separation of cross-linked elastomer (top) and ceramic (bottom) phases...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28017798/n-glycosylation-influences-the-catalytic-activity-of-mosquito-%C3%AE-glucosidases-associated-with-susceptibility-or-refractoriness-to%C3%A2-lysinibacillus-sphaericus
#11
Nathaly Alexandre do Nascimento, Lígia Maria Ferreira, Tatiany Patrícia Romão, Darleide Maria da Conceição Correia, Crhisllane Rafaele Dos Santos Vasconcelos, Antônio Mauro Rezende, Samara Graciane Costa, Fernando Ariel Genta, Osvaldo Pompílio de-Melo-Neto, Maria Helena Neves Lobo Silva-Filha
Cqm1 and Aam1 are α-glucosidases (EC 3.2.1.20) expressed in Culex quinquefasciatus and Aedes aegypti larvae midgut, respectively. These orthologs share high sequence similarity but while Cqm1 acts as a receptor for the Binary (Bin) insecticidal toxin from Lysinibacillus sphaericus, Aam1 does not bind the toxin, rendering Ae. aegypti refractory to this bacterium. Aam1 is heavily glycosylated, contrasting to Cqm1, but little is known regarding how glycosylation impacts on its function. This study aimed to compare the N-glycosylation patterns and the catalytic activities of Aam1 and Cqm1...
December 23, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28002990/translation-and-validation-of-the-perinatal-grief-scale-in-a-sample-of-greek-women-with-perinatal-loss-during-the-1-st-and-2-nd-trimester-of-pregnancy
#12
Elissavet Maniatelli, Yiannis Zervas, Panagiotis Halvatsiotis, Eirini Tsartsara, Chara Tzavara, Despina D Briana, Nikolaos Salakos
OBJECTIVE: To translate and validate the Perinatal Grief Scale (short version) in a sample of Greek women with perinatal loss during the 1(st) and 2(nd) trimester of pregnancy. METHOD: One hundred seventy-six women were approached a few hours after the loss. Along with the Perinatal Grief Scale (PGS), three more questionnaires were completed: the Edinburgh Postnatal Depression Scale (EPDS), the Hospital Anxiety and Depression Scale (HADS), the State-Trait Anxiety and Inventory (STAI), in order to assess the convergent validity of the PGS...
December 21, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27992393/manipulating-the-extracellular-matrix-an-animal-model-of-the-bladder-pain-syndrome
#13
Ifeoma Offiah, Athanasios Didangelos, Barry A OʼReilly, Stephen B McMahon
Bladder pain syndrome (BPS) is associated with breakdown of the protective uroepithelial barrier of the urinary bladder allowing urinary constituents access to bladder sensory neurons. Although there are several animal models of cystitis, none specifically relates to BPS. Here, we aimed to create such a model using enzymatic digestion of the barrier proteoglycans (PGs) in the rat. Twenty female Wistar rats were anaesthetized and transurethrally catheterized. Ten animals were treated with 0.25IU of intravesical chondroitinase ABC and heparanase III to digest chondroitin sulphate and heparin sulphate PGs, respectively...
January 2017: Pain
https://www.readbyqxmd.com/read/27989837/prophenoloxidase-activation-and-antimicrobial-peptide-expression-induced-by-the-recombinant-microbe-binding-protein-of-manduca-sexta
#14
Yang Wang, Haobo Jiang
Manduca sexta microbe binding protein (MBP) is a member of the β-1,3-glucanase-related protein superfamily that includes Gram-negative bacteria-binding proteins (GNBPs), β-1,3-glucan recognition proteins (βGRPs), and β-1,3-glucanases. Our previous and current studies showed that the purified MBP from baculovirus-infected insect cells had stimulated prophenoloxidase (proPO) activation in the hemolymph of naïve and immune challenged larvae and that supplementation of the exogenous MBP and peptidoglycans (PGs) had caused synergistic increases in PO activity...
October 28, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27987771/adhesion-and-metabolic-activity-of-human-corneal-cells-on-pcl-based-nanofiber-matrices
#15
Piotr Stafiej, Florian Küng, Daniel Thieme, Marta Czugala, Friedrich E Kruse, Dirk W Schubert, Thomas A Fuchsluger
In this work, polycaprolactone (PCL) was used as a basic polymer for electrospinning of random and aligned nanofiber matrices. Our aim was to develop a biocompatible substrate for ophthalmological application to improve wound closure in defects of the cornea as replacement for human amniotic membrane. We investigated whether blending the hydrophobic PCL with poly (glycerol sebacate) (PGS) or chitosan (CHI) improves the biocompatibility of the matrices for cell expansion. Human corneal epithelial cells (HCEp) and human corneal keratocytes (HCK) were used for in vitro biocompatibility studies...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27984141/improvement-of-activity-thermo-stability-and-fruit-juice-clarification-characteristics-of-fungal-exo-polygalacturonase
#16
Faiza Amin, Haq Nawaz Bhatti, Muhammad Bilal, Muhammad Asgher
An extracellular exo-polygalacturonase (exo-PG) from Penicillium notatum was immobilized in sodium-alginate matrix through two different protocols, viz. covalent bonding and adsorption to enhance its catalytic activity, thermal stability and life-time properties for industrial applications. Covalent immobilization was more efficient in terms of high relative activity (45.89%) and immobilization yield (71.6%) as compared to adsorption. Immobilized exo-PG derivatives displayed maximum activities at pH 5.5 and 55°C as compared to free enzyme which showed its optimum activity at pH 6...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27976870/disruption-of-the-prostaglandin-metabolome-and-characterization-of-the-pharmaceutical-exposome-in-fish-exposed-to-wastewater-treatment-works-effluent-as-revealed-by-nanoflow-nanospray-mass-spectrometry-based-metabolomics
#17
Arthur David, Anke Lange, Alaa Abdul-Sada, Charles R Tyler, Elizabeth M Hill
Fish can be exposed to a complex mixture of chemical contaminants, including pharmaceuticals, present in discharges of wastewater treatment works (WwTWs) effluents. There is little information on the effects of effluent exposure on fish metabolism, especially the small molecule signaling compounds which are the biological target of many pharmaceuticals. We applied a newly developed sensitive nanoflow-nanospray mass spectrometry nontargeted profiling technique to identify changes in the exposome and metabolome of roach (Rutilus rutilus) exposed to a final WwTWs effluent for 15 days...
January 3, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27966970/anionic-and-cationic-silver-nanoparticle-binding-restructures-net-anionic-pc-pg-monolayers-with-saturated-or-unsaturated-lipids
#18
G D Bothun, N Ganji, I A Khan, A Xi, C Bobba
We have examined the interactions between polymer-coated anionic (Ag-COOH) and cationic (Ag-NH) silver nanoparticles, and net-anionic lipid monolayers using dynamic surface pressure measurements. Monolayers composed of saturated or monounsaturated mixtures of anionic phosphatidylglycerol (PG) and zwitterionic phosphatidylcholine (PC) lipids (3:1 molar ratio) were used to determine how lipid packing and monolayer phase state influence the extent of nanoparticle binding and the monolayer response. Anionic Ag-COOH inserted into saturated dipalmitoyl-PC/PG (DPPC/DPPG) and dioleoyl-PC/PG (DOPC/DOPG) monolayers at a low initial surface pressure (10 mN m(-1)) and caused lipid condensation at high initial surface pressures (20 and 30 mN m(-1))...
December 30, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27966828/the-onset-of-puberty-in-cameroon-dwarf-goats-kept-as-pets-in-northwestern-croatia
#19
D Đuričić, S Vince, H Valpotić, I Žura Žaja, R Turk, M Lojkić, I Getz, V Berta, M Samardžija
The aim of this study was to determine onset of puberty in Cameroon Dwarf goats (CDGs) kept as pets in northwestern Croatia by determining progesterone (PGS) and insulin-like growth factor-I (IGF-I) levels in the blood by RIA methods. The first cyclic ovarian activity was estimated according to hormone profiles as determined in CDG in a moderate climate environment. Sixteen female CDG kids were kept in stables with access to pasture which provided space for exercise. The goat kids born in winter (December-January-February), spring (March-April-May), summer (June-July-August) and autumn (September-October-November) were assigned into four groups according to the season of the year of birth (n = 4 in each group)...
December 14, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27965495/use-of-the-tooth-coronal-pulp-index-for-recognition-of-the-pubertal-growth-period
#20
Fehmieh R Nawaya, Ahmad S Burhan
AIM: The present study aimed to investigate the association between the tooth coronal index (TCI) and the pubertal growth stages (PGS) for children and adolescents. MATERIALS AND METHODS: A cross-sectional study was performed using retrospectively collected panoramic and hand-wrist radiographs of 262 individuals (125 males, 137 females). The coronal height (CH) and the coronal pulp cavity height (CPCH) of the left mandibular teeth were measured. Then the TCI for which was calculated according to Ikeda et al (1985)...
November 1, 2016: Journal of Contemporary Dental Practice
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