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https://www.readbyqxmd.com/read/28934417/production-of-3-geranyl-4-hydroxybenzoate-acid-in-yeast-an-important-intermediate-of-shikonin-biosynthesis-pathway
#1
Sheng Wang, Ruishan Wang, Tan Liu, Zhilai Zhan, Liping Kang, Yanan Wang, Chaogeng Lv, Daniele Werck-Reichhart, Lanping Guo, Luqi Huang
Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain...
November 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28933274/an-overview-on-cardiovascular-protective-effects-of-catechins
#2
Samarghandian Saeed, Tahere Farkhondeh, Shaterzadeh-Yazdi Hanieh
The impact of diet strategies to manage cardiovascular disease has been focused in recent years. The food ingredients, such as flavonoids, are known as cardioprotective agents; however, there is not fully understood how these compounds are effective against cardiovascular problems. Catechins are polyphenolic compounds exhibited several biological activities in the human body, potentially in the treatment of cardiovascular disease. The present study indicates that catechins may be effective against cardiovascular problems through modulating blood lipid metabolism, protecting vascular endothelial, and decreasing blood pressure...
September 20, 2017: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#3
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28917127/differential-expression-of-ten-candidate-genes-regulating-prostaglandin-action-in-reproductive-tissues-of-buffalo-during-estrous-cycle-and-pregnancy
#4
Ankita Dillipkumar Verma, Manjit Panigrahi, Naseer Ahmad Baba, Sourabh Sulabh, Abdul Sadam, Subhashree Parida, Krishnaswamy Narayanan, Arvind A Sonwane, Bharat Bhushan
Prostaglandins (PGs) are the key mediators of several female reproductive functions, including luteolysis, ovulation, fertilization, implantation, pregnancy, and parturition. The present study was conducted in buffalo endometrial and luteal tissues between nonpregnant and two stages of pregnancy (29-38 days of pregnancy, 48-56 days of pregnancy) tissue samples. The genes involved from synthesis upto receptor level effect of PGs (PGF2α and PGE2) were studied for their relative mRNA expression. We have collected the endometrial and luteal tissues from slaughtered animals and confirmed the stages by external examination and crown vertebral rump length measurement of the foetus...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28875694/small-molecular-tgf-%C3%AE-1-inhibitor-loaded-electrospun-fibrous-scaffolds-for-preventing-hypertrophic-scars
#5
Le Wang, Junchuan Yang, Bei Ran, Xinglong Yang, Wenfu Zheng, Yunze Long, Xingyu Jiang
Hypertrophic scarring (HS) is a disorder that occurs during wound healing and seriously depresses the quality of human life. Scar-inhibiting scaffolds, though bringing promise to HS prevention, face problems such as the incompatibility of the scaffold materials and the instability of bioactive molecules. Herein, we present a TGF-β1-inhibitor-doped poly(ε-caprolactone) (PCL)/gelatin (PG) coelectrospun nanofibrous scaffold (PGT) for HS prevention during wound healing. The appropriate ratio of PCL to gelatin can avoid individual defects of the two materials and achieve an optimized mechanical property and biocompatibility...
September 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28868574/racial-minority-group-interest-in-direct-to-consumer-genetic-testing-findings-from-the-pgen-study
#6
Latrice Landry, Daiva Elena Nielsen, Deanna Alexis Carere, J Scott Roberts, Robert C Green
There is little information regarding direct-to-consumer (DTC) personal genetic testing (PGT) in non-White racial minorities. Using a web-based survey, we compared the pretest interests and attitudes toward DTC-PGT of racial minority and White DTC-PGT customers of 23andMe and Pathway Genomics using chi-square tests and multinomial regression. Data were available for 1487 participants (1389 White, 44 Black, and 54 Asian). Survey responses were similar across racial groups, although a greater proportion of Blacks compared to Whites reported being "very interested" in genetic information related to traits (91...
September 4, 2017: Journal of Community Genetics
https://www.readbyqxmd.com/read/28855281/genome-wide-association-mapping-of-stem-rust-resistance-in-hordeum-vulgare-subsp-%C3%A2-spontaneum
#7
Ahmad H Sallam, Priyanka Tyagi, Gina Brown-Guedira, Gary J Muehlbauer, Alex Hulse, Brian J Steffenson
Stem rust was one of the most devastating diseases of barley in North America. Through the deployment of cultivars with the resistance gene Rpg1, losses to stem rust have been minimal over the past 70 years. However, there exist both domestic (QCCJB) and foreign (TTKSK aka isolate Ug99) pathotypes with virulence for this important gene. To identify new sources of stem rust resistance for barley, we evaluated the Wild Barley Diversity Collection (WBDC) (314 ecogeographically diverse accessions of Hordeum vulgare subsp...
August 30, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28810664/bacterial-phosphoglycosyl-transferases-initiators-of-glycan-biosynthesis-at-the-membrane-interface
#8
Vinita Lukose, Marthe T C Walvoort, Barbara Imperiali
Phosphoglycosyl transferases (PGTs) initiate the biosynthesis of both essential and virulence-associated bacterial glycoconjugates including lipopolysaccharide, peptidoglycan and glycoproteins. PGTs catalyze the transfer of a phosphosugar moiety from a nucleoside diphosphate sugar to a polyprenol phosphate, to form a membrane-bound polyprenol diphosphosugar product. PGTs are integral membrane proteins, which include between 1 and 11 predicted transmembrane domains. Despite this variation, common motifs have been identified in PGT families through bioinformatics and mutagenesis studies...
September 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28797454/impact-of-early-prophylactic-feeding-on-long-term-tube-dependency-outcomes-in-patients-with-head-and-neck-cancer
#9
Teresa Brown, Merrilyn Banks, Brett G M Hughes, Charles Lin, Lizbeth M Kenny, Judith D Bauer
OBJECTIVES: Prophylactic gastrostomy tube (PGT) is frequently used in patients with head and neck cancer (HNSCC). There are concerns this leads to tube dependency but this phenomena is not well defined. This study aimed to determine whether early feeding via PGT impacted on longer term tube feeding outcomes. MATERIALS AND METHODS: Patients with HNSCC with PGT were observed monthly post-treatment regarding tube use and time to removal up to twelve months. Patients were from a randomised controlled trial comparing an early feeding intervention via the PGT (n=57) versus usual care which commenced feeding when clinically indicated (n=67)...
September 2017: Oral Oncology
https://www.readbyqxmd.com/read/28761141/effects-of-pulpotomy-using-mineral-trioxide-aggregate-on-prostaglandin-transporter-and-receptors-in-rat-molars
#10
Naoto Ohkura, Naoki Edanami, Ryosuke Takeuchi, Aiko Tohma, Mariko Ohkura, Nagako Yoshiba, Kunihiko Yoshiba, Hiroko Ida-Yonemochi, Hayato Ohshima, Takashi Okiji, Yuichiro Noiri
Mineral trioxide aggregate (MTA) is a commonly used dental pulp-capping material with known effects in promoting reparative dentinogenesis. However, the mechanism by which MTA induces dentine repair remains unclear. The aim of the present study was to investigate the role of prostaglandin E2 (PGE2) in dentine repair by examining the localisation and mRNA expression levels of its transporter (Pgt) and two of its receptors (Ep2 and Ep4) in a rat model of pulpotomy with MTA capping. Ep2 expression was detected in odontoblasts, endothelial cells, and nerve fibres in normal and pulpotomised tissues, whereas Pgt and Ep4 were immunolocalised only in the odontoblasts...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28749078/vitamin-d-insufficiency-correlates-with-peripheral-b10-cells-in-patients-with-pituitary-tumours
#11
Weimin Wang, Li Fu, Shengli Li, Zhiming Xu, Peng Qiu, Tong-Jiang Xu, Wei Yang, Yu-Bao Zhang, Guang-Ming Xu, Xiang-Dong Lu, Xinggang Li
The mechanism of pituitary gland tumour (PGT) is unclear. Aberrant immune tolerance is associated with the pathogenesis of tumour. Vitamin D and vitamin D receptor (VDR) are involved in the immune regulation. Interleukin (IL)-10 is one of the important immune regulatory molecules. This study aims to elucidate the role of VDR in the regulation of IL-10 in peripheral B cells of PGT patients. In this study, the peripheral blood samples were collected from PGT patients and healthy subjects. B cells were purified from the blood samples and analysed by RT-qPCR and Western blotting...
July 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28745102/inheritance-and-bulked-segregant-analysis-of-leaf-rust-and-stem-rust-resistance-in-durum-wheat-genotypes
#12
Meriem Aoun, James Kolmer, Matthew N Rouse, Shiaoman Chao, Worku Denbel Bulbula, Elias M Elias, Maricelis Acevedo
Leaf rust, caused by Puccinia triticina (Pt) and stem rust caused by Puccinia graminis f. sp. tritici (Pgt) are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to Pt-race BBBQJ and stem rust resistance (Sr genes to Pgt-race TTKSK in durum accessions. Eight leaf rust resistant genotypes were used to develop bi-parental populations. Accessions, PI 192051 and PI 534304 were also resistant to Pgt-race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage...
July 26, 2017: Phytopathology
https://www.readbyqxmd.com/read/28720918/perceived-motivating-factors-and-barriers-for-the-completion-of-postgraduate-training-among-american-pharmacy-students-prior-to-beginning-advanced-pharmacy-practice-experiences
#13
Drayton A Hammond, Douglas R Oyler, John W Devlin, Jacob T Painter, Scott Bolesta, Joseph M Swanson, Brett J Bailey, Trisha Branan, Jeffrey F Barletta, Brianne Dunn, Jason S Haney, Paul Juang, Sandra L Kane-Gill, Tyree H Kiser, Hira Shafeeq, Debra Skaar, Pamela Smithburger, Jodi Taylor
Objective. To examine perceived motivating factors and barriers (MFB) to postgraduate training (PGT) pursuit among pharmacy students. Methods. Third-year pharmacy students at 13 schools of pharmacy provided demographics and their plan and perceived MFBs for pursuing PGT. Responses were characterized using descriptive statistics. Kruskal-Wallis equality-of-proportions rank tests determined if differences in perceived MFBs existed between students based on plan to pursue PGT. Results. Among 1218 (69.5%) respondents, 37...
June 2017: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/28667570/molecular-cloning-expression-and-characterization-of-oenococcus-oeni-priming-glycosyltransferases
#14
Maria Dimopoulou, Olivier Claisse, Lucie Dutilh, Cécile Miot-Sertier, Patricia Ballestra, Patrick M Lucas, Marguerite Dols-Lafargue
Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases (pGTs). These enzymes are essential for EPS formation as they catalyze the first biosynthetic step through the formation of a phosphoanhydride bond between a hexose-1-phosphate and a lipid carrier undecaprenyl phosphate...
August 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28664315/the-wound-healing-effect-of-7-3-4-trimethoxyflavone-through-increased-levels-of-prostaglandin-e2-by-15-hydroxyprostaglandin-dehydrogenase-inhibition
#15
Kyung Hoon Sun, Sandeep Karna, Young-Sook Moon, Hoon Cho, Cheol-Hee Choi
OBJECTIVE: To find an inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) that rapidly metabolises Prostaglandin E2 (PGE2) as a mediator of wound healing, we examined seven flavonoids for this role. RESULTS: 7,3',4'-Trimethoxyflavone (TMF) had the lowest IC50 value of 0.34 µM for 15-PGDH inhibition but >400 µM for cytotoxicity, indicating a high therapeutic index. TMF elevated PGE2 levels in a concentration-dependent manner in both A549 lung cancer and HaCaT cells...
June 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28630348/analysis-of-a-dual-domain-phosphoglycosyl-transferase-reveals-a-ping-pong-mechanism-with-a-covalent-enzyme-intermediate
#16
Debasis Das, Petr Kuzmic, Barbara Imperiali
Phosphoglycosyl transferases (PGTs) are integral membrane proteins with diverse architectures that catalyze the formation of polyprenol diphosphate-linked glycans via phosphosugar transfer from a nucleotide diphosphate-sugar to a polyprenol phosphate. There are two PGT superfamilies that differ significantly in overall structure and topology. The polytopic PGT superfamily, represented by MraY and WecA, has been the subject of many studies because of its roles in peptidoglycan and O-antigen biosynthesis. In contrast, less is known about a second, extensive superfamily of PGTs that reveals a core structure with dual domain architecture featuring a C-terminal soluble globular domain and a predicted N-terminal membrane-associated domain...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28513283/molecular-characterization-of-australian-isolates-of-puccinia-graminis-f-sp-tritici-supports-long-term-clonality-but-also-reveals-cryptic-genetic-variation
#17
Jianping Zhang, Peng Zhang, Haydar Karaoglu, Robert F Park
Long-term surveys of pathogenicity in Puccinia graminis f. sp. tritici in Australia have implicated mutation as a major source of virulence, at times leading to the demise of stem-rust-resistant wheat cultivars and substantial yield losses. Since 1925, these surveys have identified at least four occasions on which exotic isolates of P. graminis f. sp. tritici appeared in Australia, with each acting as a founding isolate that gave rise sequentially to derivative pathotypes via presumed single-step mutation. The current study examined the relationship between virulence and molecular patterns using simple-sequence repeat (SSR) markers on selected isolates of P...
September 2017: Phytopathology
https://www.readbyqxmd.com/read/28512697/utilization-of-genetic-counseling-after-direct-to-consumer-genetic-testing-findings-from-the-impact-of-personal-genomics-pgen-study
#18
Diane R Koeller, Wendy R Uhlmann, Deanna Alexis Carere, Robert C Green, J Scott Roberts
Direct-to-consumer personal genomic testing (DTC-PGT) results lead some individuals to seek genetic counseling (GC), but little is known about these consumers and why they seek GC services. We analyzed survey data pre- and post-PGT from 1026 23andMe and Pathway Genomics customers. Participants were mostly white (91%), female (60%), and of high socioeconomic status (80% college educated, 43% household income of ≥$100,000). After receiving PGT results, 43 participants (4%) made or planned to schedule an appointment with a genetic counselor; 390 (38%) would have used in-person GC had it been available...
May 16, 2017: Journal of Genetic Counseling
https://www.readbyqxmd.com/read/28479736/in-vitro-wound-healing-effect-of-15-hydroxyprostaglandin-dehydrogenase-inhibitor-from-plant
#19
Sandeep Karna
BACKGROUND: Prostaglandins (PGs) have short existence in vivo because they are rapidly metabolized by NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to 15-ketoprostaglandins. Inhibition of 15-PGDH causes elevated level of PGE2 in cellular system. It will be valuable for the therapeutic management of diseases requiring elevated PGE2 levels, like wound healing. OBJECTIVE: Ninety-eight plant samples were screened for the discovery of potent 15-PGDH inhibitor...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28464943/diet-and-exercise-changes-following-direct-to-consumer-personal-genomic-testing
#20
Daiva Elena Nielsen, Deanna Alexis Carere, Catharine Wang, J Scott Roberts, Robert C Green
BACKGROUND: The impacts of direct-to-consumer personal genomic testing (PGT) on health behaviors such as diet and exercise are poorly understood. Our investigation aimed to evaluate diet and exercise changes following PGT and to determine if changes were associated with genetic test results obtained from PGT. METHODS: Customers of 23andMe and Pathway Genomics completed a web-based survey prior to receiving PGT results (baseline) and 6 months post-results. Fruit and vegetable intake (servings/day), and light, vigorous and strength exercise frequency (days/week) were assessed...
May 2, 2017: BMC Medical Genomics
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