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https://www.readbyqxmd.com/read/29154474/intracrine-prostaglandin-e2-pro-tumoral-actions-in-prostate-epithelial-cells-originate-from-non-canonical-pathways
#1
Antonio Madrigal-Martínez, Ana B Fernández-Martínez, Francisco J Lucio Cazaña
Prostaglandin E2 (PGE2 ) increases cell proliferation and stimulates migratory and angiogenic abilities in prostate cancer cells. However, the effects of PGE2 on non-transformed prostate epithelial cells are unknown, despite the fact that PGE2 overproduction has been found in benign hyperplastic prostates. In the present work we studied the effects of PGE2 in immortalized, non-malignant prostate epithelial RWPE-1 cells and found that PGE2 increased cell proliferation, cell migration, and production of vascular endothelial growth factor-A, and activated in vitro angiogenesis...
November 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29120706/administration-of-cloprostenol-and-oxytocin-before-electroejaculation-in-goat-bucks-reduces-the-needed-amount-of-electrical-stimulation-without-affecting-seminal-quality
#2
Rodolfo Ungerfeld, Daniela Casuriaga, Julia Giriboni, Aline Freitas-de-Melo, Patricia Silveira, Felipe Zandonadi Brandão
Electroejaculation (EE) is a widely used semen collection technique; but, it is stressful and painful for the animals. Considering these concerns, it may be important to develop practices to decrease the negative implications of EE on animals. Oxytocin and prostaglandin-F2alpha (PGF2α) stimulate the contractions of the muscles of the male genital tract. Therefore, the aim of this study was to analyze the effectiveness of the administration of oxytocin and/or a PGF2α analogue (cloprostenol) to bucks in relation to their stress response and sperm parameters before semen collection by EE was performed...
October 28, 2017: Theriogenology
https://www.readbyqxmd.com/read/29095157/pteridine-glycosyltransferase-from-chlorobium-tepidum-crystallization-and-x-ray-analysis
#3
Asaithambi Killivalavan, Young Shik Park, Kon Ho Lee
The pteridine glycosyltransferase (PGT) found in Chlorobium tepidum (CtPGT) catalyzes the conversion of L-threo-tetrahydrobiopterin to 1-O-(L-threo-biopterin-2'-yl)-β-N-acetylglucosamine using UDP-N-acetylglucosamine. The gene for CtPGT was cloned, and selenomethionine-derivatized protein was overexpressed and purified using various chromatographic techniques. The protein was crystallized by the hanging-drop vapour-diffusion method using 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350 as a reservoir solution...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29078294/identification-and-characterization-of-sr13-a-tetraploid-wheat-gene-that-confers-resistance-to-the-ug99-stem-rust-race-group
#4
Wenjun Zhang, Shisheng Chen, Zewdie Abate, Jayaveeramuthu Nirmala, Matthew N Rouse, Jorge Dubcovsky
The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13...
October 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29046334/the-organic-anion-transporter-slco2a1-constitutes-the-core-component-of-the-maxi-cl-channel
#5
Ravshan Z Sabirov, Petr G Merzlyak, Toshiaki Okada, Md Rafiqul Islam, Hiromi Uramoto, Tomoko Mori, Yumiko Makino, Hiroshi Matsuura, Yu Xie, Yasunobu Okada
The maxi-anion channels (MACs) are expressed in cells from mammals to amphibians with ~60% exhibiting a phenotype called Maxi-Cl. Maxi-Cl serves as the most efficient pathway for regulated fluxes of inorganic and organic anions including ATP However, its molecular entity has long been elusive. By subjecting proteins isolated from bleb membranes rich in Maxi-Cl activity to LC-MS/MS combined with targeted siRNA screening, CRISPR/Cas9-mediated knockout, and heterologous overexpression, we identified the organic anion transporter SLCO2A1, known as a prostaglandin transporter (PGT), as a key component of Maxi-Cl...
October 18, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29040520/clinical-implications-of-mitochondrial-dna-quantification-on-pregnancy-outcomes-a-blinded-prospective-non-selection-study
#6
Elpida Fragouli, Caroline McCaffrey, Krithika Ravichandran, Katharina Spath, James A Grifo, Santiago Munné, Dagan Wells
STUDY QUESTION: Can quantification of mitochondrial DNA (mtDNA) in trophectoderm (TE) biopsy samples provide information concerning the viability of a blastocyst, potentially enhancing embryo selection and improving IVF treatment outcomes? SUMMARY ANSWER: This study demonstrated that euploid blastocysts of good morphology, but with high mtDNA levels had a greatly reduced implantation potential. WHAT IS KNOWN ALREADY: Better methods of embryo selection leading to IVF outcome improvement are necessary, as the transfer of chromosomally normal embryos of high morphological grade cannot guarantee the establishment of an ongoing pregnancy...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29039978/preimplantation-genetic-testing-current-challenges-and-future-prospects
#7
Anver Kuliev, Svetlana Rechitsky
Preimplantation genetic testing (PGT) is now a widely applied procedure in genetic practices and ART, with more than one third of ART Centers in US already utilizing PGT technology. Its indications have also been significantly extended to include common late-onset disorders and non-genetic conditions, such as testing for HLA match. Areas covered: This is a critical review of the developments in PGT, with emphasis on their outstanding limitations and directions for the future research and practice in the area of PGT...
November 8, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29031889/outcomes-important-to-burns-patients-during-scar-management-and-how-they-compare-to-the-concepts-captured-in-burn-specific-patient-reported-outcome-measures
#8
Laura L Jones, Melanie Calvert, Naiem Moiemen, Jonathan J Deeks, Jonathan Bishop, Philip Kinghorn, Jonathan Mathers
BACKGROUND: Pressure garment therapy (PGT) is an established treatment for the prevention and treatment of hypertrophic scarring; however, there is limited evidence for its effectiveness. Burn survivors often experience multiple issues many of which are not adequately captured in current PGT trial measures. To assess the effectiveness of PGT it is important to understand what outcomes matter to patients and to consider whether patient-reported outcome measures (PROMs) can be used to ascertain the effect of treatments on patients' health-related quality of life...
October 11, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29031888/below-the-surface-parents-views-on-the-factors-that-influence-treatment-adherence-in-paediatric-burn-scar-management-a-qualitative-study
#9
Nicole Andrews, Laura L Jones, Naiem Moiemen, Melanie Calvert, Philip Kinghorn, Ian Litchfield, Jonathan Bishop, Jonathan J Deeks, Jonathan Mathers
INTRODUCTION: Parents have a crucial role to play in burn scar management for their children at a time that is extremely stressful for them and their child. Scar management treatments such as pressure garment therapy (PGT) require high levels of adherence. There has been a lack of research into the factors that may influence adherence in paediatric burn scar management. This qualitative research study has investigated parents' experiences of scar management and their attempts to adhere to treatment at home...
October 11, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28985908/effect-of-the-male-factor-on-the-clinical-outcome-of-intracytoplasmic-sperm-injection-combined-with-preimplantation-aneuploidy-testing-observational-longitudinal-cohort-study-of-1-219-consecutive-cycles
#10
Rossella Mazzilli, Danilo Cimadomo, Alberto Vaiarelli, Antonio Capalbo, Lisa Dovere, Erminia Alviggi, Ludovica Dusi, Carlo Foresta, Francesco Lombardo, Andrea Lenzi, Herman Tournaye, Carlo Alviggi, Laura Rienzi, Filippo Maria Ubaldi
OBJECTIVE: To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). DESIGN: Observational longitudinal cohort study. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA)...
October 3, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28947769/quantifying-airborne-dispersal-routes-of-pathogens-over-continents-to-safeguard-global-wheat-supply
#11
M Meyer, J A Cox, M D T Hitchings, L Burgin, M C Hort, D P Hodson, C A Gilligan
Infectious crop diseases spreading over large agricultural areas pose a threat to food security. Aggressive strains of the obligate pathogenic fungus Puccinia graminis f.sp. tritici (Pgt), causing the crop disease wheat stem rust, have been detected in East Africa and the Middle East, where they lead to substantial economic losses and threaten livelihoods of farmers. The majority of commercially grown wheat cultivars worldwide are susceptible to these emerging strains, which pose a risk to global wheat production, because the fungal spores transmitting the disease can be wind-dispersed over regions and even continents (1-11) ...
September 25, 2017: Nature Plants
https://www.readbyqxmd.com/read/28934417/production-of-3-geranyl-4-hydroxybenzoate-acid-in-yeast-an-important-intermediate-of-shikonin-biosynthesis-pathway
#12
Sheng Wang, Ruishan Wang, Tan Liu, Zhilai Zhan, Liping Kang, Yanan Wang, Chaogeng Lv, Daniele Werck-Reichhart, Lanping Guo, Luqi Huang
Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain...
November 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28933274/an-overview-on-cardiovascular-protective-effects-of-catechins
#13
Saeed Samarghandian, Tahere Farkhondeh, Hanieh Shaterzadeh-Yazdi
The impact of diet strategies to manage cardiovascular disease has been focused in recent years. The food ingredients, such as flavonoids, are known as cardioprotective agents; however, there is not fully understood how these compounds are effective against cardiovascular problems. Catechins are polyphenolic compounds exhibited several biological activities in the human body, potentially in the treatment of cardiovascular disease. The present study indicates that catechins may be effective against cardiovascular problems through modulating blood lipid metabolism, protecting vascular endothelial, and decreasing blood pressure...
September 20, 2017: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#14
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28917127/differential-expression-of-ten-candidate-genes-regulating-prostaglandin-action-in-reproductive-tissues-of-buffalo-during-estrous-cycle-and-pregnancy
#15
Ankita Dillipkumar Verma, Manjit Panigrahi, Naseer Ahmad Baba, Sourabh Sulabh, Abdul Sadam, Subhashree Parida, Krishnaswamy Narayanan, Arvind A Sonwane, Bharat Bhushan
Prostaglandins (PGs) are the key mediators of several female reproductive functions, including luteolysis, ovulation, fertilization, implantation, pregnancy, and parturition. The present study was conducted in buffalo endometrial and luteal tissues between nonpregnant and two stages of pregnancy (29-38 days of pregnancy, 48-56 days of pregnancy) tissue samples. The genes involved from synthesis upto receptor level effect of PGs (PGF2α and PGE2) were studied for their relative mRNA expression. We have collected the endometrial and luteal tissues from slaughtered animals and confirmed the stages by external examination and crown vertebral rump length measurement of the foetus...
January 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/28875694/small-molecular-tgf-%C3%AE-1-inhibitor-loaded-electrospun-fibrous-scaffolds-for-preventing-hypertrophic-scars
#16
Le Wang, Junchuan Yang, Bei Ran, Xinglong Yang, Wenfu Zheng, Yunze Long, Xingyu Jiang
Hypertrophic scarring (HS) is a disorder that occurs during wound healing and seriously depresses the quality of human life. Scar-inhibiting scaffolds, though bringing promise to HS prevention, face problems such as the incompatibility of the scaffold materials and the instability of bioactive molecules. Herein, we present a TGF-β1-inhibitor-doped poly(ε-caprolactone) (PCL)/gelatin (PG) coelectrospun nanofibrous scaffold (PGT) for HS prevention during wound healing. The appropriate ratio of PCL to gelatin can avoid individual defects of the two materials and achieve an optimized mechanical property and biocompatibility...
September 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28868574/racial-minority-group-interest-in-direct-to-consumer-genetic-testing-findings-from-the-pgen-study
#17
Latrice Landry, Daiva Elena Nielsen, Deanna Alexis Carere, J Scott Roberts, Robert C Green
There is little information regarding direct-to-consumer (DTC) personal genetic testing (PGT) in non-White racial minorities. Using a web-based survey, we compared the pretest interests and attitudes toward DTC-PGT of racial minority and White DTC-PGT customers of 23andMe and Pathway Genomics using chi-square tests and multinomial regression. Data were available for 1487 participants (1389 White, 44 Black, and 54 Asian). Survey responses were similar across racial groups, although a greater proportion of Blacks compared to Whites reported being "very interested" in genetic information related to traits (91...
October 2017: Journal of Community Genetics
https://www.readbyqxmd.com/read/28855281/genome-wide-association-mapping-of-stem-rust-resistance-in-hordeum-vulgare-subsp-spontaneum
#18
Ahmad H Sallam, Priyanka Tyagi, Gina Brown-Guedira, Gary J Muehlbauer, Alex Hulse, Brian J Steffenson
Stem rust was one of the most devastating diseases of barley in North America. Through the deployment of cultivars with the resistance gene Rpg1, losses to stem rust have been minimal over the past 70 yr. However, there exist both domestic (QCCJB) and foreign (TTKSK aka isolate Ug99) pathotypes with virulence for this important gene. To identify new sources of stem rust resistance for barley, we evaluated the Wild Barley Diversity Collection (WBDC) (314 ecogeographically diverse accessions of Hordeum vulgare subsp...
October 5, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28810664/bacterial-phosphoglycosyl-transferases-initiators-of-glycan-biosynthesis-at-the-membrane-interface
#19
Vinita Lukose, Marthe T C Walvoort, Barbara Imperiali
Phosphoglycosyl transferases (PGTs) initiate the biosynthesis of both essential and virulence-associated bacterial glycoconjugates including lipopolysaccharide, peptidoglycan and glycoproteins. PGTs catalyze the transfer of a phosphosugar moiety from a nucleoside diphosphate sugar to a polyprenol phosphate, to form a membrane-bound polyprenol diphosphosugar product. PGTs are integral membrane proteins, which include between 1 and 11 predicted transmembrane domains. Despite this variation, common motifs have been identified in PGT families through bioinformatics and mutagenesis studies...
September 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28797454/impact-of-early-prophylactic-feeding-on-long-term-tube-dependency-outcomes-in-patients-with-head-and-neck-cancer
#20
Teresa Brown, Merrilyn Banks, Brett G M Hughes, Charles Lin, Lizbeth M Kenny, Judith D Bauer
OBJECTIVES: Prophylactic gastrostomy tube (PGT) is frequently used in patients with head and neck cancer (HNSCC). There are concerns this leads to tube dependency but this phenomena is not well defined. This study aimed to determine whether early feeding via PGT impacted on longer term tube feeding outcomes. MATERIALS AND METHODS: Patients with HNSCC with PGT were observed monthly post-treatment regarding tube use and time to removal up to twelve months. Patients were from a randomised controlled trial comparing an early feeding intervention via the PGT (n=57) versus usual care which commenced feeding when clinically indicated (n=67)...
September 2017: Oral Oncology
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