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https://www.readbyqxmd.com/read/29204966/roles-of-organic-anion-transporting-polypeptide-2a1-oatp2a1-slco2a1-in-regulating-the-pathophysiological-actions-of-prostaglandins
#1
Takeo Nakanishi, Ikumi Tamai
Solute carrier organic anion transporter family member 2A1 (OATP2A1, encoded by the SLCO2A1 gene), which was initially identified as prostaglandin transporter (PGT), is expressed ubiquitously in tissues and mediates the distribution of prostanoids, such as PGE2, PGF2α, PGD2 and TxB2. It is well known to play a key role in the metabolic clearance of prostaglandins, which are taken up into the cell by OATP2A1 and then oxidatively inactivated by 15-ketoprostaglandin dehydrogenase (encoded by HPGD); indeed, OATP2A1-mediated uptake is the rate-limiting step of PGE2 catabolism...
December 4, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29177535/genome-wide-association-study-of-stem-rust-resistance-in-a-world-collection-of-cultivated-barley
#2
Austin J Case, Sridhar Bhavani, Godwin Macharia, Brian J Steffenson
QTL conferring a 14-40% reduction in adult plant stem rust severity to multiple races of Pgt were found on chromosome 5H and will be useful in barley breeding. Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt) is an important disease of barley. The resistance gene Rpg1 has protected the crop against stem rust losses for over 70 years in North America, but is not effective against the African Pgt race TTKSK (and its variants) nor the domestic race QCCJB. To identify resistance to these Rpg1-virulent races, the Barley iCore Collection, held by the United States Department of Agriculture-Agricultural Research Service National Small Grains Collection was evaluated for adult plant resistance (APR) and seedling resistance to race TTKSK and APR to race QCCJB and the Pgt TTKSK composite of races TTKSK, TTKST, TTKTK, and TTKTT...
November 24, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29164644/should-embryos-with-autosomal-monosomy-by-preimplantation-genetic-testing-for-aneuploidy-be-transferred-implications-for-embryo-selection-from-a-systematic-literature-review-of-autosomal-monosomy-survivors
#3
REVIEW
M E Bunnell, L Wilkins-Haug, R Reiss
OBJECTIVE: To review the literature for survival and phenotypes of liveborns with autosomal monosomy to inform decisions regarding transfer of IVF-derived embryos reported as monosomic on preimplantation genetic testing for aneuploidy (PGT-A). METHOD: Ovid-Medline and EMBASE were systematically searched to identify published case reports of live-born individuals with autosomal monosomy, full or mosaic, for a whole chromosome. RESULTS: Fifty-three reports describing 56 individuals with autosomal monosomy met the selection criteria: one case each of monosomy 14 and 16, three each for monosomy 15 and 18, one for group "E", five for monosomy 20, twenty-four for monosomy 21, seven for monosomy 22, and eleven for a "G" group chromosome...
November 21, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29164273/mapping-and-characterization-of-wheat-stem-rust%C3%A2-resistance-genes-srtm5-and-sr60-from-triticum-monococcum
#4
Shisheng Chen, Yan Guo, Jordan Briggs, Felix Dubach, Shiaoman Chao, Wenjun Zhang, Matthew N Rouse, Jorge Dubcovsky
The new stem rust resistance gene Sr60 was fine-mapped to the distal region of chromosome arm 5A(m)S, and the TTKSK-effective gene SrTm5 could be a new allele of Sr22. The emergence and spread of new virulent races of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici; Pgt), including the Ug99 race group, is a serious threat to global wheat production. In this study, we mapped and characterized two stem rust resistance genes from diploid wheat Triticum monococcum accession PI 306540. We mapped SrTm5, a previously postulated gene effective to Ug99, on chromosome arm 7A(m)L, completely linked to Sr22...
November 21, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29154474/intracrine-prostaglandin-e2-pro-tumoral-actions-in-prostate-epithelial-cells-originate-from-non-canonical-pathways
#5
Antonio Madrigal-Martínez, Ana B Fernández-Martínez, Francisco J Lucio Cazaña
Prostaglandin E2 (PGE2 ) increases cell proliferation and stimulates migratory and angiogenic abilities in prostate cancer cells. However, the effects of PGE2 on non-transformed prostate epithelial cells are unknown, despite the fact that PGE2 overproduction has been found in benign hyperplastic prostates. In the present work we studied the effects of PGE2 in immortalized, non-malignant prostate epithelial RWPE-1 cells and found that PGE2 increased cell proliferation, cell migration, and production of vascular endothelial growth factor-A, and activated in vitro angiogenesis...
November 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29120706/administration-of-cloprostenol-and-oxytocin-before-electroejaculation-in-goat-bucks-reduces-the-needed-amount-of-electrical-stimulation-without-affecting-seminal-quality
#6
Rodolfo Ungerfeld, Daniela Casuriaga, Julia Giriboni, Aline Freitas-de-Melo, Patricia Silveira, Felipe Zandonadi Brandão
Electroejaculation (EE) is a widely used semen collection technique; but, it is stressful and painful for the animals. Considering these concerns, it may be important to develop practices to decrease the negative implications of EE on animals. Oxytocin and prostaglandin-F2alpha (PGF2α) stimulate the contractions of the muscles of the male genital tract. Therefore, the aim of this study was to analyze the effectiveness of the administration of oxytocin and/or a PGF2α analogue (cloprostenol) to bucks in relation to their stress response and sperm parameters before semen collection by EE was performed...
October 28, 2017: Theriogenology
https://www.readbyqxmd.com/read/29095157/pteridine-glycosyltransferase-from-chlorobium-tepidum-crystallization-and-x-ray-analysis
#7
Asaithambi Killivalavan, Young Shik Park, Kon Ho Lee
The pteridine glycosyltransferase (PGT) found in Chlorobium tepidum (CtPGT) catalyzes the conversion of L-threo-tetrahydrobiopterin to 1-O-(L-threo-biopterin-2'-yl)-β-N-acetylglucosamine using UDP-N-acetylglucosamine. The gene for CtPGT was cloned, and selenomethionine-derivatized protein was overexpressed and purified using various chromatographic techniques. The protein was crystallized by the hanging-drop vapour-diffusion method using 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350 as a reservoir solution...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29078294/identification-and-characterization-of-sr13-a-tetraploid-wheat-gene-that-confers-resistance-to-the-ug99-stem-rust-race-group
#8
Wenjun Zhang, Shisheng Chen, Zewdie Abate, Jayaveeramuthu Nirmala, Matthew N Rouse, Jorge Dubcovsky
The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13...
November 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29046334/the-organic-anion-transporter-slco2a1-constitutes-the-core-component-of-the-maxi-cl-channel
#9
Ravshan Z Sabirov, Petr G Merzlyak, Toshiaki Okada, Md Rafiqul Islam, Hiromi Uramoto, Tomoko Mori, Yumiko Makino, Hiroshi Matsuura, Yu Xie, Yasunobu Okada
The maxi-anion channels (MACs) are expressed in cells from mammals to amphibians with ~60% exhibiting a phenotype called Maxi-Cl. Maxi-Cl serves as the most efficient pathway for regulated fluxes of inorganic and organic anions including ATP However, its molecular entity has long been elusive. By subjecting proteins isolated from bleb membranes rich in Maxi-Cl activity to LC-MS/MS combined with targeted siRNA screening, CRISPR/Cas9-mediated knockout, and heterologous overexpression, we identified the organic anion transporter SLCO2A1, known as a prostaglandin transporter (PGT), as a key component of Maxi-Cl...
November 15, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29040520/clinical-implications-of-mitochondrial-dna-quantification-on-pregnancy-outcomes-a-blinded-prospective-non-selection-study
#10
Elpida Fragouli, Caroline McCaffrey, Krithika Ravichandran, Katharina Spath, James A Grifo, Santiago Munné, Dagan Wells
STUDY QUESTION: Can quantification of mitochondrial DNA (mtDNA) in trophectoderm (TE) biopsy samples provide information concerning the viability of a blastocyst, potentially enhancing embryo selection and improving IVF treatment outcomes? SUMMARY ANSWER: This study demonstrated that euploid blastocysts of good morphology, but with high mtDNA levels had a greatly reduced implantation potential. WHAT IS KNOWN ALREADY: Better methods of embryo selection leading to IVF outcome improvement are necessary, as the transfer of chromosomally normal embryos of high morphological grade cannot guarantee the establishment of an ongoing pregnancy...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29039978/preimplantation-genetic-testing-current-challenges-and-future-prospects
#11
Anver Kuliev, Svetlana Rechitsky
Preimplantation genetic testing (PGT) is now a widely applied procedure in genetic practices and ART, with more than one third of ART Centers in US already utilizing PGT technology. Its indications have also been significantly extended to include common late-onset disorders and non-genetic conditions, such as testing for HLA match. Areas covered: This is a critical review of the developments in PGT, with emphasis on their outstanding limitations and directions for the future research and practice in the area of PGT...
November 8, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29031889/outcomes-important-to-burns-patients-during-scar-management-and-how-they-compare-to-the-concepts-captured-in-burn-specific-patient-reported-outcome-measures
#12
Laura L Jones, Melanie Calvert, Naiem Moiemen, Jonathan J Deeks, Jonathan Bishop, Philip Kinghorn, Jonathan Mathers
BACKGROUND: Pressure garment therapy (PGT) is an established treatment for the prevention and treatment of hypertrophic scarring; however, there is limited evidence for its effectiveness. Burn survivors often experience multiple issues many of which are not adequately captured in current PGT trial measures. To assess the effectiveness of PGT it is important to understand what outcomes matter to patients and to consider whether patient-reported outcome measures (PROMs) can be used to ascertain the effect of treatments on patients' health-related quality of life...
October 12, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29031888/below-the-surface-parents-views-on-the-factors-that-influence-treatment-adherence-in-paediatric-burn-scar-management-a-qualitative-study
#13
Nicole Andrews, Laura L Jones, Naiem Moiemen, Melanie Calvert, Philip Kinghorn, Ian Litchfield, Jonathan Bishop, Jonathan J Deeks, Jonathan Mathers
INTRODUCTION: Parents have a crucial role to play in burn scar management for their children at a time that is extremely stressful for them and their child. Scar management treatments such as pressure garment therapy (PGT) require high levels of adherence. There has been a lack of research into the factors that may influence adherence in paediatric burn scar management. This qualitative research study has investigated parents' experiences of scar management and their attempts to adhere to treatment at home...
October 11, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28985908/effect-of-the-male-factor-on-the-clinical-outcome-of-intracytoplasmic-sperm-injection-combined-with-preimplantation-aneuploidy-testing-observational-longitudinal-cohort-study-of-1-219-consecutive-cycles
#14
Rossella Mazzilli, Danilo Cimadomo, Alberto Vaiarelli, Antonio Capalbo, Lisa Dovere, Erminia Alviggi, Ludovica Dusi, Carlo Foresta, Francesco Lombardo, Andrea Lenzi, Herman Tournaye, Carlo Alviggi, Laura Rienzi, Filippo Maria Ubaldi
OBJECTIVE: To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). DESIGN: Observational longitudinal cohort study. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA)...
October 3, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28947769/quantifying-airborne-dispersal-routes-of-pathogens-over-continents-to-safeguard-global-wheat-supply
#15
M Meyer, J A Cox, M D T Hitchings, L Burgin, M C Hort, D P Hodson, C A Gilligan
Infectious crop diseases spreading over large agricultural areas pose a threat to food security. Aggressive strains of the obligate pathogenic fungus Puccinia graminis f.sp. tritici (Pgt), causing the crop disease wheat stem rust, have been detected in East Africa and the Middle East, where they lead to substantial economic losses and threaten livelihoods of farmers. The majority of commercially grown wheat cultivars worldwide are susceptible to these emerging strains, which pose a risk to global wheat production, because the fungal spores transmitting the disease can be wind-dispersed over regions and even continents (1-11) ...
September 25, 2017: Nature Plants
https://www.readbyqxmd.com/read/28934417/production-of-3-geranyl-4-hydroxybenzoate-acid-in-yeast-an-important-intermediate-of-shikonin-biosynthesis-pathway
#16
Sheng Wang, Ruishan Wang, Tan Liu, Zhilai Zhan, Liping Kang, Yanan Wang, Chaogeng Lv, Daniele Werck-Reichhart, Lanping Guo, Luqi Huang
Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain...
November 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28933274/an-overview-on-cardiovascular-protective-effects-of-catechins
#17
Saeed Samarghandian, Tahere Farkhondeh, Hanieh Shaterzadeh-Yazdi
The impact of diet strategies to manage cardiovascular disease has been focused in recent years. The food ingredients, such as flavonoids, are known as cardioprotective agents; however, there is not fully understood how these compounds are effective against cardiovascular problems. Catechins are polyphenolic compounds exhibited several biological activities in the human body, potentially in the treatment of cardiovascular disease. The present study indicates that catechins may be effective against cardiovascular problems through modulating blood lipid metabolism, protecting vascular endothelial, and decreasing blood pressure...
September 20, 2017: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#18
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28917127/differential-expression-of-ten-candidate-genes-regulating-prostaglandin-action-in-reproductive-tissues-of-buffalo-during-estrous-cycle-and-pregnancy
#19
Ankita Dillipkumar Verma, Manjit Panigrahi, Naseer Ahmad Baba, Sourabh Sulabh, Abdul Sadam, Subhashree Parida, Krishnaswamy Narayanan, Arvind A Sonwane, Bharat Bhushan
Prostaglandins (PGs) are the key mediators of several female reproductive functions, including luteolysis, ovulation, fertilization, implantation, pregnancy, and parturition. The present study was conducted in buffalo endometrial and luteal tissues between nonpregnant and two stages of pregnancy (29-38 days of pregnancy, 48-56 days of pregnancy) tissue samples. The genes involved from synthesis upto receptor level effect of PGs (PGF2α and PGE2) were studied for their relative mRNA expression. We have collected the endometrial and luteal tissues from slaughtered animals and confirmed the stages by external examination and crown vertebral rump length measurement of the foetus...
January 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/28875694/small-molecular-tgf-%C3%AE-1-inhibitor-loaded-electrospun-fibrous-scaffolds-for-preventing-hypertrophic-scars
#20
Le Wang, Junchuan Yang, Bei Ran, Xinglong Yang, Wenfu Zheng, Yunze Long, Xingyu Jiang
Hypertrophic scarring (HS) is a disorder that occurs during wound healing and seriously depresses the quality of human life. Scar-inhibiting scaffolds, though bringing promise to HS prevention, face problems such as the incompatibility of the scaffold materials and the instability of bioactive molecules. Herein, we present a TGF-β1-inhibitor-doped poly(ε-caprolactone) (PCL)/gelatin (PG) coelectrospun nanofibrous scaffold (PGT) for HS prevention during wound healing. The appropriate ratio of PCL to gelatin can avoid individual defects of the two materials and achieve an optimized mechanical property and biocompatibility...
September 27, 2017: ACS Applied Materials & Interfaces
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