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Olga Michel, Dawid Przystupski, Jolanta Saczko, Anna Szewczyk, Natalia Niedzielska, Joanna Rossowska, Julita Kulbacka
Until recently, green tea polyphenols were considered strong antioxidants. However, the latest reports have revealed that bioflavonoids can play a multiple role in anticancer therapy, including the inhibition of cell proliferation and generation of the oxidative stress in a dose-dependent manner. The presented research was designed to examine the potential of the green tea (±)-catechin as a reinforcement of the electrochemotherapy (ECT) with cisplatin in pancreatic cancer in vitro. The study was performed on two cell lines of the pancreatic ductal adenocarcinoma (PDA) - parental EPP85-181P and multidrug-resistant EPP85-181RNOV...
May 22, 2018: Acta Biochimica Polonica
R Babona-Pilipos, N Liu, A Pritchard-Oh, A Mok, D Badawi, M R Popovic, C M Morshead
Neural precursor cells (NPCs) respond to externally applied direct current electrical fields (DCEFs) by undergoing rapid and directed migration toward the cathode in a process known as galvanotaxis. It is unknown if the underlying mechanisms of galvanotactic migration is common to non-electrosensitive cells and if so, how NPCs and other galvanotactic cells sense and transduce electrical fields into cellular motility. In this study, we show that distinct aspects of NPC galvanotactic migration: motility (quantified through |velocity|) and directedness, are differentially regulated by calcium...
July 15, 2018: Experimental Cell Research
Tara Bahadur K C, Seiichi Tada, Liping Zhu, Takanori Uzawa, Noriko Minagawa, Shyh-Chyang Luo, Haichao Zhao, Hsiao-Hua Yu, Toshiro Aigaki, Yoshihiro Ito
An electrosensitive peptide probe has been developed from an in vitro selection technique using biorthogonal tRNA prepared with an electroreactive non-natural amino acid, 3,4-ethylenedioxythiophene-conjugated aminophenylalanine. The selected probe quantitatively detected the influenza virus based on a signal "turn-on" mechanism. The developed strategy could be used to develop electrochemical biosensors toward a variety of targets.
May 17, 2018: Chemical Communications: Chem Comm
Xing Zhang, Ke Xia, Lei Lin, Fuming Zhang, Yanlei Yu, Kalib St Ange, Xiaorui Han, Eric Edsinger, Joel Sohn, Robert J Linhardt
The skate, a cartilaginous fish related to sharks and rays, possesses a unique electrosensitive sensory organ known as the ampullae of Lorenzini (AoL). This organ is responsible for the detection of weak electric field changes caused by the muscle contractions of their prey. While keratan sulfate (KS) is believed to be a component of a jelly that fills this sensory organ and has been credited with its high proton conductivity, modern analytical methods have not been applied to its characterization. Surprisingly, total glycosaminoglycan (GAG) analysis demonstrates that the KS from skate jelly is extraordinarily pure, containing no other GAGs...
June 15, 2018: ACS Chemical Biology
Lars Chittka
Maeterlinck did not mean to suggest that honeybees rival humans in intelligence - rather he saw in the bee a qualitatively different form of intelligence, tailored to the challenges of a profoundly different kind of society and lifestyle. Insects are strange "aliens from inner space", with sensory and cognitive worlds wholly different from our own. The 19(th) century discovery that ants can detect ultraviolet light triggered a golden age in the exploration of the diversity of sensory systems of insects (and indeed other animals), identifying such abilities as magnetic compasses, electrosensitivity, polarization vision, and peculiar locations for sense organs such as the infrared sensors on the abdomens of some beetles or photoreceptors on the genitalia of some butterflies...
October 9, 2017: Current Biology: CB
Sarah D Jensen, Vera A Khorokhorina, Claudia Muratori, Andrei G Pakhomov, Olga N Pakhomova
We demonstrate that conditioning of mammalian cells by electroporation with nanosecond pulsed electric field (nsPEF) facilitates their response to the next nsPEF treatment. The experiments were designed to unambiguously separate the electroporation-induced sensitization and desensitization effects. Electroporation was achieved by bursts of 300-ns, 9 kV/cm pulses (50 Hz, n = 3-100) and quantified by propidium dye uptake within 11 min after the nsPEF exposure. We observed either sensitization to nsPEF or no change (when the conditioning was either too weak or too intense, or when the wait time after conditioning was too short)...
September 8, 2017: Scientific Reports
Zsuzsanna Dömötör, Renáta Szemerszky, Ferenc Köteles
Although nature relatedness is considered a positive characteristic, its relationship to constructs involving worries about the negative effects of artificial environmental factors is also feasible. A questionnaire assessing modern health worries, electrosensitivity, somatosensory amplification, spirituality, and nature relatedness was completed by 510 individuals. Nature relatedness was related to electrosensitivity, modern health worries, and spirituality. In a binary logistic regression analysis, somatosensory amplification, modern health worries, and nature relatedness were associated with electrosensitivity, and nature relatedness moderated the connection between modern health worries and electrosensitivity...
March 1, 2017: Journal of Health Psychology
Claudia Muratori, Andrei G Pakhomov, Loree Heller, Maura Casciola, Elena Gianulis, Sergey Grigoryev, Shu Xiao, O N Pakhomova
Nanosecond pulsed electric fields are emerging as a new modality for tissue and tumor ablation. We previously reported that cells exposed to pulsed electric fields develop hypersensitivity to subsequent pulsed electric field applications. This phenomenon, named electrosensitization, is evoked by splitting the pulsed electric field treatment in fractions (split-dose treatments) and causes in vitro a 2- to 3-fold increase in cytotoxicity. The aim of this study was to show the benefit of split-dose treatments for in vivo tumor ablation by nanosecond pulsed electric field...
January 1, 2017: Technology in Cancer Research & Treatment
Kumaran Thiruppathy, John Mason, Khalid Akbari, Amanda Raeburn, Anton Emmanuel
OBJECTIVE: Fecal incontinence (FI) and constipation can arise from a variety of alterations of anorectal function. This study aimed to investigate the components of the anorecal reflex in patients with these symptoms and to determine the functional significance of various physiological parameters. METHODS: Altogether 21 healthy volunteers (controls) and 78 FI-predominant and 74 constipation-predominant patients were recruited and administered Wexner incontinence and constipation questionnaires...
April 2017: Journal of Digestive Diseases
Janja Dermol, Olga N Pakhomova, Andrei G Pakhomov, Damijan Miklavčič
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified...
2016: PloS One
Ajda Prevc, Apolonija Bedina Zavec, Maja Cemazar, Veronika Kloboves-Prevodnik, Monika Stimac, Vesna Todorovic, Primoz Strojan, Gregor Sersa
Bystander effect, a known phenomenon in radiation biology, where irradiated cells release signals which cause damage to nearby, unirradiated cells, has not been explored in electroporated cells yet. Therefore, our aim was to determine whether bystander effect is present in electroporated melanoma cells in vitro, by determining viability of non-electroporated cells exposed to medium from electroporated cells and by the release of microvesicles as potential indicators of the bystander effect. Here, we demonstrated that electroporation of cells induces bystander effect: Cells exposed to electric pulses mediated their damage to the non-electroporated cells, thus decreasing cell viability...
October 2016: Journal of Membrane Biology
V Passananti, A Wilton, G Preziosi, J B Storrie, A Emmanuel
BACKGROUND: Multiple sclerosis (MS) is the commonest disabling neurological disease in young adults. A majority of patients experience bowel dysfunction, reporting a wide spectrum of bowel symptoms that significantly negatively impact social activities and emotional state. Transanal irrigation (TAI) is a method of managing such bowel symptoms. We aimed to investigate long-term efficacy of TAI, to measure health status-related quality of life and identify factors predictive of TAI outcome...
September 2016: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
Claudia Muratori, Andrei G Pakhomov, Shu Xiao, Olga N Pakhomova
Previous studies reported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mammalian cells that had been subjected to electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspensions when the electric pulse treatments were split in fractions. The present study was aimed to visualize the effect of sensitization and establish its utility for cell ablation. We used KLN 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel...
March 18, 2016: Scientific Reports
Luisa Nascimento Medeiros, Tanit Ganz Sanchez
INTRODUCTION: Tinnitus is a multifactorial condition and its prevalence has increased on the past decades. The worldwide progressive increase of the use of cell phones has exposed the peripheral auditory pathways to a higher dose of electromagnetic radiofrequency radiation (EMRFR). Some tinnitus patients report that the abusive use of mobiles, especially when repeated in the same ear, might worsen ipsilateral tinnitus. OBJECTIVE: The aim of this study was to evaluate the available evidence about the possible causal association between tinnitus and exposure to electromagnetic waves...
January 2016: Brazilian Journal of Otorhinolaryngology
Maxime M van Meegdenburg, Erik Heineman, Paul M A Broens
BACKGROUND: Conscious external anal sphincter contraction is mediated by the pudendal nerve. Pudendal neuropathy is, therefore, believed to result in fecal incontinence. Until urge sensation is experienced, fecal continence is maintained by unconscious external anal sphincter contraction, which is regulated by the anal-external sphincter continence reflex. The innervation of unconscious contraction is yet unknown. OBJECTIVE: We aimed to determine whether unconscious contraction is mediated by the pudendal nerve and whether age influences unconscious contraction...
December 2015: Diseases of the Colon and Rectum
Cindy Sage
The 'informational content' of Earth's electromagnetic signaling is like a set of operating instructions for human life. These environmental cues are dynamic and involve exquisitely low inputs (intensities) of critical frequencies with which all life on Earth evolved. Circadian and other temporal biological rhythms depend on these fluctuating electromagnetic inputs to direct gene expression, cell communication and metabolism, neural development, brainwave activity, neural synchrony, a diversity of immune functions, sleep and wake cycles, behavior and cognition...
2015: Reviews on Environmental Health
Reuben S Son, Thiruvallur R Gowrishankar, Kyle C Smith, James C Weaver
Pulse trains are widely used in electroporation (EP) for both general biomedical research and clinical applications such as nonthermal tumor ablation. Here we use a computational method based on a meshed transport network to investigate a cell system model's response to a train of identical, evenly spaced electric field pulses. We obtain an unexpected result: the number of membrane pores decreases during the application of twenty 1.0 kV/cm, 100 μs pulses, delivered at 1 Hz. This pulse train initially creates 13,000 membrane pores, but pore number decreases by a factor of 15 to about 830 pores throughout subsequent pulses...
March 2016: IEEE Transactions on Bio-medical Engineering
Renáta Szemerszky, Mónika Gubányi, Dorottya Árvai, Zsuzsanna Dömötör, Ferenc Köteles
BACKGROUND: Among people with idiopathic environmental intolerance attributed to electromagnetic fields (IEI-EMF), a better than random detection ability for a 50-Hz 0.5-mT magnetic field (MF) and a propensity to experience more symptoms than controls was reported in a previous study. PURPOSE: The current study aimed to replicate and clarify these results using a modified experimental design. METHOD: Participants of the provocation experiment were 49 individuals with self-reported IEI-EMF and 57 controls...
December 2015: International Journal of Behavioral Medicine
Rachel M Berquist, Vitaly L Galinsky, Stephen M Kajiura, Lawrence R Frank
The cartilaginous and non-neopterygian bony fishes have an electric sense typically comprised of hundreds or thousands of sensory canals distributed in broad clusters over the head. This morphology facilitates neural encoding of local electric field intensity, orientation, and polarity, used for determining the position of nearby prey. The coelacanth rostral organ electric sense, however, is unique in having only three paired sensory canals with distribution restricted to the dorsal snout, raising questions about its function...
2015: Scientific Reports
Francesco Sannicolò, Patrizia R Mussini, Tiziana Benincori, Roberto Cirilli, Sergio Abbate, Serena Arnaboldi, Simone Casolo, Ettore Castiglioni, Giovanna Longhi, Rocco Martinazzo, Monica Panigati, Marco Pappini, Elsa Quartapelle Procopio, Simona Rizzo
Invited for the cover of this issue is Francesco Sannicolò and co-workers at different Italian institutions, who all contributed to the different specific areas of this multidisciplinary work. The image depicts inherently chiral elliptical and triangular oligothiophene macrocycles and their symmetrical resemblance to mysterious crop circles. Read the full text of the article at 10.1002/chem.201404331.
November 17, 2014: Chemistry: a European Journal
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