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Tuberculosis biomarker

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https://www.readbyqxmd.com/read/28097879/metabolomics-biomarkers-for-tuberculosis-diagnostics-current-status-and-future-objectives
#1
Ilse du Preez, Laneke Luies, Du Toit Loots
Numerous studies have contributed to our current understanding of the complex biology of pulmonary tuberculosis and subsequently provided solutions to its control or eradication. Metabolomics, a newcomer to the Omics research domain, has significantly contributed to this understanding by identifying biomarkers originating from the disease-associated metabolome adaptations of both the microbe and host. These biomarkers have shed light on previously unknown disease mechanisms, many of which have been implemented toward the development of improved diagnostic strategies...
January 18, 2017: Biomarkers in Medicine
https://www.readbyqxmd.com/read/28087649/identification-of-unique-essential-proteins-from-a-m-tuberculosis-f15-lam4-kzn-phage-secretome-library
#2
Thamsanqa Emmanuel Chiliza, Manormoney Pillay, Balakrishna Pillay
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis disease (TB), the leading cause of death from bacterial infection worldwide. Although treatable, the resurgence of multi- and extensively-drug resistance TB is a major setback for the fight against TB globally. Consequently, there is an urgent need for new Mtb derived biomarkers for use in the design of new drugs and rapid point-of-care diagnostic or prognostic tools for management of TB transmission. Therefore, the present study aimed to identify unique Mtb secreted proteins from the extensively drug resistant Mtb F15/LAM4/KZN phage secretome library...
January 12, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28077246/testing-of-a-palatable-bait-and-compatible-vaccine-carrier-for-the-oral-vaccination-of-european-badgers-meles-meles-against-tuberculosis
#3
Sonya Gowtage, Gareth A Williams, Ray Henderson, Paul Aylett, Duncan MacMorran, Si Palmer, Andy Robertson, Sandrine Lesellier, Stephen P Carter, Mark A Chambers
The oral vaccination of wild badgers (Meles meles) with live Bacillus Calmette-Guérin (BCG) is one of the tools being considered for the control of bovine tuberculosis (caused by Mycobacterium bovis) in the UK. The design of a product for oral vaccination requires that numerous, and often competing, conditions are met. These include the need for a highly palatable, but physically stable bait that will meet regulatory requirements, and one which is also compatible with the vaccine formulation; in this case live BCG...
January 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28068297/fatty-acid-derivative-chemokine-and-cytokine-profiles-in-exhaled-breath-condensates-can-differentiate-adult-and-children-paucibacillary-tuberculosis-patients
#4
Sergio Fabián Mosquera-Restrepo, Ana Cecilia Caro, Luis F García, Carlos Alberto Peláez-Jaramillo, Mauricio Rojas
The anti-mycobacterial immune response in adults and children with tuberculosis (TB), as well as the response in bacteriologically positive and negative patients, is different. However, knowledge of the immunological events occurring in the lungs in these clinical situations remains scarce. Exhaled breath condensate (EBC) samples may be useful for studying the inflammatory environment of the lower airways in TB patients. The fatty acid, cytokine, and chemokine profiles in EBC from healthy adults; smear-positive and smear-negative adult patients; and healthy, asthmatic, and TB children were determined using gas chromatography and LUMINEX, respectively...
January 9, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28065665/unbiased-identification-of-blood-based-biomarkers-for-pulmonary-tuberculosis-by-modeling-and-mining-molecular-interaction-networks
#5
Awanti Sambarey, Abhinandan Devaprasad, Abhilash Mohan, Asma Ahmed, Soumya Nayak, Soumya Swaminathan, George D'Souza, Anto Jesuraj, Chirag Dhar, Subash Babu, Annapurna Vyakarnam, Nagasuma Chandra
Efficient diagnosis of tuberculosis (TB) is met with multiple challenges, calling for a shift of focus from pathogen-centric diagnostics towards identification of host-based multi-marker signatures. Transcriptomics offer a list of differentially expressed genes, but cannot by itself identify the most influential contributors to the disease phenotype. Here, we describe a computational pipeline that adopts an unbiased approach to identify a biomarker signature. Data from RNA sequencing from whole blood samples of TB patients were integrated with a curated genome-wide molecular interaction network, from which we obtain a comprehensive perspective of variations that occur in the host due to TB...
February 2017: EBioMedicine
https://www.readbyqxmd.com/read/28063937/cerebrospinal-fluid-metabolomic-profiling-in-tuberculous-and-viral-meningitis-screening-potential-markers-for-differential-diagnosis
#6
Zihui Li, Boping Du, Jing Li, Jinli Zhang, Xiaojing Zheng, Hongyan Jia, Aiying Xing, Qi Sun, Fei Liu, Zongde Zhang
BACKGROUND: Tuberculous meningitis (TBM) is the most severe and frequent form of central nervous system tuberculosis. The current lack of efficient diagnostic tests makes it difficult to differentiate TBM from other common types of meningitis, especially viral meningitis (VM). Metabolomics is an important tool to identify disease-specific biomarkers. However, little metabolomic information is available on adult TBM. METHODS: We used (1)H nuclear magnetic resonance-based metabolomics to investigate the metabolic features of the CSF from 18 TBM and 20 VM patients...
January 4, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28061955/the-role-of-cd30-and-cd153-cd30l-in-the-anti-mycobacterial-immune-response
#7
REVIEW
Nancy D Marín, Luis F García
The establishment of a protective T-cell response against mycobacterial infections involves different co-stimulatory molecules and their respective ligands. Among these molecules the Tumor Necrosis Factor Receptor Super-family (TNFRSF) and the Tumor Necrosis Factor Super-family (TNFSF) are known to be important members. This review analyzes the evidence that CD30 and CD153 (CD30L), members of the TNFRSF and TNSF, play key roles in the T cell-dependent anti-mycobacterial immune response. Mice deficient in either CD30 or CD153, or treated with antibodies blocking the effects or CD30 and CD153, and infected with M...
January 2017: Tuberculosis
https://www.readbyqxmd.com/read/28061954/multiple-cytokine-responses-in-discriminating-between-active-tuberculosis-and-latent-tuberculosis-infection
#8
Jing Wu, Sen Wang, Chanyi Lu, Lingyun Shao, Yan Gao, Zumo Zhou, Heqing Huang, Ying Zhang, Wenhong Zhang
BACKGROUND: Cytokines play an important role in cell-mediated immune responses against Mycobacterium tuberculosis (Mtb) infection. Cytokine profile specifically associated with active tuberculosis (ATB) patients, subjects with latent tuberculosis infection (LTBI) and non-infected individuals remains to be determined. METHODS: We enrolled a total of 92 subjects including patients with ATB (n = 25), LTBI (n = 36) and healthy controls (HC, n = 31) to investigate the cytokine production by peripheral blood mononuclear cells after Mtb purified protein derivative (PPD) stimulation which was evaluated by a beads-based multiplex assay system...
January 2017: Tuberculosis
https://www.readbyqxmd.com/read/28061948/levels-of-microrna-mir-16-and-mir-155-are-altered-in-serum-of-patients-with-tuberculosis-and-associate-with-responses-to-therapy
#9
Vishal Wagh, Anant Urhekar, Deepak Modi
Identification of blood biomarkers that can be useful for predicting Mycobacterium tuberculosis (M.TB) infection, effect of therapy and Multi Drug Resistant (MDR) TB infected individuals is clinically useful for combating tuberculosis epidemic. In this study, we have evaluated the levels of selected miRNAs in serum of TB and MDR TB patients. In addition, we have studied their levels in serum of patients post-therapy. The levels of 4-miRNAs (miR-16, miR-29a, miR-125b and miR-155) were measured in 30 newly diagnosed TB patients, 19 Multi Drug Resistant (MDR) TB patients, 10 patients who completed TB therapy and were TB negative...
January 2017: Tuberculosis
https://www.readbyqxmd.com/read/28059955/diagnosis-of-opportunistic-infections-hiv-co-infections-tuberculosis
#10
Lesley Scott, Pedro da Silva, Catharina C Boehme, Wendy Stevens, Chris Gilpin
PURPOSE OF REVIEW: Tuberculosis (TB) incidence has declined ∼1.5% annually since 2000, but continued to affect 10.4 million individuals in 2015, with 1/3 remaining undiagnosed or underreported. The diagnosis of TB among those co-infected with HIV is challenging as TB remains the leading cause of death in such individuals. Accurate and rapid diagnosis of active TB will avert mortality in both adults and children, reduce transmission, and assist in timeous decisions for antiretroviral therapy initiation...
January 5, 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/28057604/biological-markers-in-the-diagnosis-of-tuberculous-pleural-effusion
#11
Bastien Mollo, Stéphane Jouveshomme, François Philippart, Benoît Pilmis
Tuberculosis is one of the main etiologies to evoke in the context of lymphocyte pleurisy. However, diagnosis is difficult and is based on mycobacteriology that is not enough sensitive and time-consuming, or on histology that requires invasive biopsy gesture. This literature review, carried out from Medline, summarizes the main meta-analyzes, reviews, and originator publications in English on biomarkers, classic and more innovative, studied for the diagnosis of tuberculous pleurisy. Among the immuno-biochemical markers, interferon-γ (IFN-γ), isoenzyme of adenosine deaminase 2 (ADA2) and total adenosine deaminase (ADA) seem the most relevant with respective sensitivities of 89% (87-91), 97...
January 5, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28054932/hiv-infection-rather-than-concurrent-opportunistic-infections-drives-most-systemic-procoagulant-vascular-and-damage-responses-a-prospective-cohort-study-in-central-africa
#12
Saskia Janssen, Michaela Am Huson, Kara K Osbak, Elie-Gide Rossatanga, Abraham Alabi, René Lutter, Martin P Grobusch, Tom van der Poll
BACKGROUND: HIV infection is accompanied by various systemic host responses, including activation of coagulation and the vascular endothelium. We sought to determine the impact of opportunistic co-infections in a Central-African setting. METHODS: This prospective study included 98 HIV-infected individuals in Gabon initiating combination antiretroviral therapy (cART) and followed them up for 6 months. Patients were stratified according to the presence of active tuberculosis (TB; n=19), mucocutaneous opportunistic infection (n=9), or no opportunistic infection (n=70)...
January 5, 2017: Antiviral Therapy
https://www.readbyqxmd.com/read/28049128/immune-reconstitution-inflammatory-syndrome-associated-with-pulmonary-pathogens
#13
REVIEW
Radha Gopal, Rekha R Rapaka, Jay K Kolls
Immune reconstitution inflammatory syndrome (IRIS) is an exaggerated immune response to a variety of pathogens in response to antiretroviral therapy-mediated recovery of the immune system in HIV-infected patients. Although IRIS can occur in many organs, pulmonary IRIS, associated with opportunistic infections such as Mycobacterium tuberculosis and Pneumocystis jirovecii, is particularly associated with high morbidity and mortality. The pathology of IRIS is associated with a variety of innate and adaptive immune factors, including CD4(+) T-cells, CD8(+) T-cells, γδ T-cells, natural killer cells, macrophages, the complement system and surfactant proteins, Toll-like receptors and pro-inflammatory cytokines and chemokines...
January 2017: European Respiratory Review: An Official Journal of the European Respiratory Society
https://www.readbyqxmd.com/read/28043542/the-analysis-of-exosomal-micro-rnas-in-peripheral-blood-mononuclear-cell-derived-macrophages-after-infection-with-bacillus-calmette-gu%C3%A3-rin-by-rna-sequencing
#14
Esmaeil Mortaz, Shamila D Alipoor, Payam Tabarsi, Ian M Adcock, Johan Garssen, Ali Akbar Velayati
OBJECTIVE/BACKGROUND: Tuberculosis (TB) is a major global threat to human health, especially in low-income countries. The diagnosis of TB is challenging because of the limitations of specificity and sensitivity with the current diagnostics. Novel, selective biomarkers for TB would be of great practical value. Exosomes are bioactive vesicles with 30-100nm in diameter, which are secreted from almost all cell types and are found in virtually every human body fluid. Exosomes transport micro-RNAs (miRNAs), which are post-transcriptional regulators of gene expression, around the body and allow miRNAs to modulate biological pathways in target cells...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043508/proteome-scale-mdr-tb-antibody-responses-for-identification-of-putative-biomarkers-for-the-diagnosis-of-drug-resistant-mycobacterium-tuberculosis
#15
Shamsi Yari, Alireza Hadizadeh Tasbiti, Mostafa Ghanei, Seyed Davar Siadat, Fatemeh Yari, A Bahrmand
OBJECTIVE: Multidrug-resistant tuberculosis (MDR-TB) is caused by Mycobacterium tuberculosis strains that do not respond to isoniazid and rifampicin, the two most effective first-line anti-TB drugs. Here, we designed and produced antibodies based on biomarkers that exist only in MDR-TB. METHODS: Bacilli were cultured for 4weeks at 37°C, and protein extraction was performed by sequential extraction. Bacterial cells were sonicated, centrifuged at 5000rpm for 45min, and the supernatant was collected and subjected to multiple rounds of treatment to prior to protein isolation...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043495/development-of-rapid-immuno-diagnostic-test-for-the-early-detection-of-tuberculosis
#16
Anil Kumar Gupta, Saumya Srivastava, Amit Singh, Sarman Singh
AIMS AND OBJECTIVE: To develop novel immunodiagnostic test for tuberculosis. METHODS: The selected novel proteins named as Rv2145c (SS1), Rv1437 (SS2), Rv1827 (SS3), and Rv2970c (SS4) were cloned, expressed and purified under specific conditions. Additionally, monoclonal antibodies (mAbs) were developed using these recombinant antigens via hybridoma technology. Hybridoma clones were screened and positive clones were selected for further experiment. The mAbs were purified from cell culture supernatant using protein A/G column chromatography...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28042217/performance-of-xpert-mtb-rif-on-ascitic-fluid-samples-for-detection-of-abdominal-tuberculosis
#17
Syed Beenish Rufai, Sarman Singh, Amit Singh, Parveen Kumar, Jitendra Singh, Anand Vishal
BACKGROUND: Diagnosis of abdominal tuberculosis (TB) from ascitic fluid samples using routinely available diagnostic methods is challenging due to its paucibacillary nature. Although performance of Xpert MTB/RIF assay has been evaluated extensively on pulmonary samples, its performance on extrapulmonary samples is still under evaluation. OBJECTIVES: The objective of this study was to find out the performance of Xpert MTB/RIF on ascitic fluid samples obtained from suspected cases of abdominal TB...
January 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28007659/retrospective-study-of-clinical-and-lesion-characteristics-of-patients-undergoing-surgical-treatment-for-pulmonary-tuberculosis-in-georgia
#18
REVIEW
Sergo Vashakidze, Albert Despuig, Shota Gogishvili, Keti Nikolaishvili, Natalia Shubladze, Zaza Avaliani, Nestan Tukvadze, Martí Casals, Joan A Caylà, Pere-Joan Cardona, Cristina Vilaplana
OBJECTIVES: Our aim was to retrospectively compare clinical data and characteristics of removed lesions of the cohort of patients undergoing therapeutical surgery for their tuberculosis. DESIGN AND METHODS: Demographic and epidemiological details, clinical data, data on the surgery performed, macroscopic characteristics of the TB lesions removed, and outcome were recorded retrospectively from the 137 patients who underwent therapeutical surgery for their TB in Tbilisi, Georgia during 2014 and 2015...
December 19, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28007592/colostrum-proinflammatory-cytokines-as-biomarkers-of-bovine-immune-response-to-bovine-tuberculosis-btb
#19
Eduardo Sánchez-Soto, Rosa Ponce-Ramos, Rodolfo Hernández-Gutiérrez, Abel Gutiérrez-Ortega, Angel H Álvarez, Moisés Martínez-Velázquez, Angel E Absalón, Pablo Ortiz-Lazareno, Alberto Limón-Flores, Ciro Estrada-Chávez, Sara E Herrera-Rodríguez
Bovine colostrum contains compounds, which provide passive immune protection from mother to newborn calves. Little is known about cytokine levels and their role in bovine colostrum. Moreover, the capacity of bovine colostrum cells to mount specific immune responses after natural exposure to bovine tuberculosis (bTB) antigens in dairy herds has not been studied, thus far. The purpose of this study was to identify biomarkers for bTB infection measurable in bovine colostrum. The present study reveals that isolated-immune colostrum cells can mount a specific immune response against bTB antigens, by measuring the novo IFN-γ release in cell culture...
December 19, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27997555/exploration-of-serum-proteomic-profiling-and-diagnostic-model-that-differentiate-crohn-s-disease-and-intestinal-tuberculosis
#20
Fenming Zhang, Chengfu Xu, Longgui Ning, Fengling Hu, Guodong Shan, Hongtan Chen, Ming Yang, Wenguo Chen, Jiekai Yu, Guoqiang Xu
AIM: To explore the diagnostic models of Crohn's disease (CD), Intestinal tuberculosis (ITB) and the differential diagnostic model between CD and ITB by analyzing serum proteome profiles. METHODS: Serum proteome profiles from 30 CD patients, 21 ITB patients and 30 healthy controls (HCs) were analyzed by using weak cationic magnetic beads combined with MALDI-TOF-MS technique to detect the differentially expressed proteins of serum samples. Three groups were made and compared accordingly: group of CD patients and HCs, group of ITB patients and HCs, group of CD patients and ITB patients...
2016: PloS One
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