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MMP-9 and dry eye

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https://www.readbyqxmd.com/read/28147407/-significance-of-new-methods-of-examining-the-tear-film-in-dry-eye-disease-tear-film-osmolarity-and-matrix-metalloproteinases-mmp-9
#1
M Schargus, G Geerling, S C Joachim
Dry eye disease is one of the most common diagnoses in ophthalmology. A variety of subjective and objective test methods with imprecise limits and highly different sensitivities and specificities complicate the diagnosis. Especially mild to moderate pathological findings can be misdiagnosed. In recent years, new options have opened, through the development of new, clinical practicable diagnostic equipment, for example, for the analysis of tear film osmolarity or matrix metalloproteinases (MMP-9). The value of tear film osmolarity and determination of MMP-9 levels in the diagnosis of dry eye and its subtypes will be discussed and evaluated objectively, on the basis of published study results...
February 1, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/27920494/presence-or-absence-of-ocular-surface-inflammation-directs-clinical-and-therapeutic-management-of-dry-eye
#2
Robert Sambursky
BACKGROUND: The presence of clinically significant inflammation has been confirmed in the tears of 40%-65% of patients with symptoms of dry eye. Ocular surface inflammation may lead to tear film instability, epithelial cell irregularities, and permeability, resulting in chronic symptomatic pain and fluctuating vision as well as negative surgical outcomes. PATIENTS AND METHODS: A retrospective single center medical chart review of 100 patients was conducted. All patients were tested with the InflammaDry test to determine if patients exhibited elevated levels of matrix metalloproteinase 9 (MMP-9)...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27914947/synthesis-and-binding-monitoring-of-a-new-nanomolar-pamam-based-matrix-metalloproteinases-inhibitor-mmpis
#3
Linda Cerofolini, Veronica Baldoneschi, Elisa Dragoni, Andrea Storai, Marianna Mamusa, Debora Berti, Marco Fragai, Barbara Richichi, Cristina Nativi
Dendrimers are efficient drug delivery systems particularly useful in ocular diseases. In particular, low generation PAMAM dendrimers are non-toxic and non-immunogenic and they provide an enhancement of the residence time of drugs in the eyes. In this context, the synthesis of the PAMAM-based matrix metalloproteinases inhibitor 5, is reported. In particular, we demonstrated that 5 strongly binds (18.0nM±2.5nM) MMP-9, the most relevant MMP responsible of ocular surface damages in induced dry eyes syndrome (DES)...
January 15, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27665213/matrix-metalloproteinase-9-testing-in-dry-eye-disease-using-a-commercially-available-point-of-care-immunoassay
#4
Elisabeth M Messmer, Victoria von Lindenfels, Alexandra Garbe, Anselm Kampik
PURPOSE: To measure matrix metalloproteinase 9 (MMP-9) in the tear film of patients with dry eye disease (DED) compared with controls and to correlate clinical findings. DESIGN: In a prospective study, 101 patients and controls underwent MMP-9 testing of the tear film. Thereafter, they were evaluated for symptoms and signs of DED. PARTICIPANTS: Included patients were those who showed 3 of the following 4 dry eye criteria: ocular surface disease index (OSDI) score of more than 12, tear film break-up time (TBUT) of 10 seconds or less, Schirmer test results without anesthesia of less than 10 mm/5 minutes, and corneal staining results of 1 or more...
November 2016: Ophthalmology
https://www.readbyqxmd.com/read/27442314/effect-of-oral-re-esterified-omega-3-nutritional-supplementation-on-dry-eyes
#5
Alice T Epitropoulos, Eric D Donnenfeld, Zubin A Shah, Edward J Holland, Michael Gross, William J Faulkner, Cynthia Matossian, Stephen S Lane, Melissa Toyos, Frank A Bucci, Henry D Perry
PURPOSE: To assess the effect of oral re-esterified omega-3 fatty acids on tear osmolarity, matrix metalloproteinase-9 (MMP-9), tear break-up time (TBUT), Ocular Surface Disease Index (OSDI), fluorescein corneal staining, Schirmer score, meibomian gland dysfunction (MGD) stage and omega-3 index in subjects with dry eyes and confirmed MGD. METHODS: This was a multicenter, prospective, interventional, placebo-controlled, double-masked study. Subjects were randomized to receive 4 softgels containing a total of 1680 mg of eicosapentaenoic acid/560 mg of docosahexaenoic acid or a control of 3136 mg of linoleic acid, daily for 12 weeks...
September 2016: Cornea
https://www.readbyqxmd.com/read/27327581/dexamethasone-drug-eluting-nanowafers-control-inflammation-in-alkali-burned-corneas-associated-with-dry-eye
#6
Fang Bian, Crystal S Shin, Changjun Wang, Stephen C Pflugfelder, Ghanashyam Acharya, Cintia S De Paiva
PURPOSE: To evaluate the efficacy of a controlled release dexamethasone delivery system for suppressing inflammation in an ocular burn + desiccating stress (OB+DS) model. METHODS: Nanowafers (NW) loaded with Dexamethasone (Dex, 10 μg) or vehicles (2.5% Methylcellulose; MC) were fabricated using hydrogel template strategy. C57BL/6 mice were subjected to unilateral alkali ocular burn with concomitant desiccating stress for 2 or 5 days and topically treated either with 2 μL of 0...
June 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26995142/optimization-and-validation-of-an-existing-surgical-and-robust-dry-eye-rat-model-for-the-evaluation-of-therapeutic-compounds
#7
Cedric Joossen, Ellen Lanckacker, Nadia Zakaria, Carina Koppen, Jurgen Joossens, Nathalie Cools, Ingrid De Meester, Anne-Marie Lambeir, Peter Delputte, Louis Maes, Paul Cos
The aim of this research was to optimize and validate an animal model for dry eye, adopting clinically relevant evaluation parameters. Dry eye was induced in female Wistar rats by surgical removal of the exorbital lacrimal gland. The clinical manifestations of dry eye were evaluated by tear volume measurements, corneal fluorescein staining, cytokine measurements in tear fluid, MMP-9 mRNA expression and CD3(+) cell infiltration in the conjunctiva. The animal model was validated by treatment with Restasis(®) (4 weeks) and commercial dexamethasone eye drops (2 weeks)...
May 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/26923552/mmp-8-is-critical-for-dexamethasone-therapy-in-alkali-burned-corneas-under-dry-eye-conditions
#8
Fang Bian, Changjun Wang, Johanna Tukler-Henriksson, Stephen C Pflugfelder, Caterina Camodeca, Elisa Nuti, Armando Rossello, De-Quan Li, Cintia S de Paiva
Our previous studies have shown that Dexamethasone (Dex) reduced the expression of matrix-metalloproteinases (MMPs -1,-3,-9,-13), IL-1β and IL-6, while it significantly increased MMP-8 mRNA transcripts in a concomitant dry eye and corneal alkali burn murine model (CM). To investigate if MMP-8 induction is responsible for some of the protective effects of Dex in CM, MMP-8 knock out mice (MMP-8KO) were subjected to the CM for 2 or 5 days and topically treated either with 2 μl of 0.1% Dexamethasone (Dex), or saline QID...
November 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/26904272/high-levels-of-17%C3%AE-estradiol-are-associated-with-increased-matrix-metalloproteinase-2-and-metalloproteinase-9-activity-in-tears-of-postmenopausal-women-with-dry-eye
#9
Guanglin Shen, Xiaoping Ma
Purpose. To determine the serum levels of sex steroids and tear matrix metalloproteinases (MMP) 2 and 9 concentrations in postmenopausal women with dry eye. Methods. Forty-four postmenopausal women with dry eye and 22 asymptomatic controls were enrolled. Blood was drawn and analyzed for serum levels of sex steroids and lipids. Then, the following tests were performed: tear collection, Ocular Surface Disease Index (OSDI) questionnaire, fluorescein tear film break-up time (TBUT), corneal fluorescein staining, Schirmer test, and conjunctival impression cytology...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/26850527/the-matrix-metalloproteinase-9-point-of-care-test%C3%A2-in-dry-eye
#10
REVIEW
Nicole L Lanza, Felipe Valenzuela, Victor L Perez, Anat Galor
Dry eye is a common, multifactorial disease currently diagnosed by a combination of symptoms and signs. However, the subjective symptoms of dry eye poorly correlate to the current gold standard for diagnostic tests, reflecting the need to develop better objective tests for the diagnosis of dry eye. This review considers the role of ocular surface matrix metalloproteinase 9 (MMP-9) in dry eye and the implications of a novel point-of-care test that measures MMP-9 levels, InflammaDry (RPS, Sarasota, FL) on choosing appropriate therapeutic treatments...
April 2016: Ocular Surface
https://www.readbyqxmd.com/read/26807724/dry-eye-profiles-in-patients-with-a-positive-elevated-surface-matrix-metalloproteinase-9-point-of-care-test-versus-negative-patients
#11
Nicole L Lanza, Allison L McClellan, Hatim Batawi, Elizabeth R Felix, Konstantinos D Sarantopoulos, Roy C Levitt, Anat Galor
PURPOSE: To compare dry eye (DE) symptoms and signs in subjects who tested positive versus those who tested negative for ocular surface matrix metalloproteinase 9 (MMP-9) using the InflammaDry point-of-care test (RPS, Sarasota, FL). METHODS: In this cross-sectional study, individuals seen in the Miami Veterans Affairs eye clinic with DE symptoms, as evidenced by DE questionnaire 5 (DEQ5) ≥6, were given standardized questionnaires to assess DE symptoms and ocular and non-ocular pain complaints...
April 2016: Ocular Surface
https://www.readbyqxmd.com/read/26774908/effects-of-punctal-occlusion-on-clinical-signs-and-symptoms-and-on-tear-cytokine-levels-in-patients-with-dry-eye
#12
Louis Tong, Roger Beuerman, Susan Simonyi, David A Hollander, Michael E Stern
PURPOSE: To investigate changes in signs, symptoms, and tear cytokines following punctal plug occlusion in patients with dry eye. METHODS: A single-center study was conducted at Singapore Eye Research Institute. Nonabsorbable punctal plugs were inserted in the lower punctum of both eyes in patients with moderate dry eye. Over 3 weeks, in the more severe eye, dry eye symptoms, fluorescein corneal staining, Schirmer I (without topical anesthesia) test, tear film breakup time (TFBUT), and safety were assessed...
April 2016: Ocular Surface
https://www.readbyqxmd.com/read/26772899/differential-effects-of-dexamethasone-and-doxycycline-on-inflammation-and-mmp-production-in-murine-alkali-burned-corneas-associated-with-dry-eye
#13
Fang Bian, Flavia S A Pelegrino, Johanna Tukler Henriksson, Stephen C Pflugfelder, Eugene A Volpe, De-Quan Li, Cintia S de Paiva
Alkali burns to the cornea are among the most devastating injuries to the eye. The purpose of this study was to evaluate the effects of dexamethasone (Dex) or doxycycline (Doxy) on protease activity and corneal complications in a combined model (CM) of alkali burn and dry eye. C57BL/6 mice were subjected to the CM for 2 or 5 days (D). Mice were topically treated either with Dex (0.1%), Dox (0.025%) or vehicle QID and observed daily for appearance of corneal perforation. Quantitative real time PCR was performed to measure expression of inflammation cytokines and matrix metalloproteinases (MMPs) in whole cornea lysates...
April 2016: Ocular Surface
https://www.readbyqxmd.com/read/26762881/blueberry-component-pterostilbene-protects-corneal-epithelial-cells-from-inflammation-via-anti-oxidative-pathway
#14
Jin Li, Ruzhi Deng, Xia Hua, Lili Zhang, Fan Lu, Terry G Coursey, Stephen C Pflugfelder, De-Quan Li
Blueberries have been recognized to possess protective properties from inflammation and various diseases, but not for eye and ocular disorders. This study explores potential benefits of pterostilbene (PS), a natural component of blueberries, in preventing ocular surface inflammation using an in vitro culture model of human corneal epithelial cells (HCECs) exposed to hyperosmotic medium at 450 mOsM. Gene expression was detected by RT-qPCR, and protein production or activity was determined by ELISA, zymography, Western blotting and immunofluorescent staining...
January 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/26682569/evaluation-of-point-of-care-test-for-elevated-tear-matrix-metalloproteinase-9-in-post-lasik-dry-eyes
#15
Tommy C Y Chan, Cong Ye, Kwok Ping Chan, Kai On Chu, Vishal Jhanji
AIM: To evaluate the performance of a point-of-care test for detection of matrix metalloproteinase 9 (MMP-9) levels in post-laser-assisted in situ keratomileusis (LASIK) dry eyes. METHODS: A comparative study between patients with mild to moderate post-LASIK dry eyes and age-matched normal subjects was conducted. Ocular surface disease index (OSDI), tear break-up time (TBUT), and tear film MMP-9 and total protein levels were compared between the two groups. A point-of-care test device (RPS InflammaDry, Sarasota, Florida, USA) was utilised to confirm elevated MMP-9 levels in tear film...
September 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/26657697/short-term-effect-of-scleral-lens-on-the-dry-eye-biomarkers-in-keratoconus
#16
Gonzalo Carracedo, Maria Serramito Blanco, Alba Martin-Gil, Wang Zicheng, Jesús Carballo Alvarez, Jesús Pintor
PURPOSE: To evaluate the most important signs of dry eye, such as osmolarity, inflammation, and diadenosine tetraphosphate (Ap(4)A) concentration before and after wearing scleral lenses for 8 h in keratoconus patients. METHODS: A pilot, experimental, short-term study involved 26 keratoconus patients (average age, 36.95 ± 8.95 years). They voluntarily enrolled in the study at the Optometry Clinic of the Faculty of Optics and Optometry in the University Complutense of Madrid...
February 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/26604661/effects-of-chondrocyte-derived-extracellular-matrix-in-a-dry-eye-mouse-model
#17
Chae Eun Kim, Ha Na Oh, Ji Hyun Lee, Jae Wook Yang
PURPOSE: The occurrence of repetitive dry eye is accompanied by inflammation. This study investigated the anti-inflammatory effects of chondrocyte-derived extracellular matrix (CDECM) on the cornea and conjunctiva in a dry eye mouse model. METHODS: Dry eyes were experimentally induced in 12- to 16-week-old NOD.B10.H2(b) mice (Control) via subcutaneous injections of scopolamine (muscarinic receptor blocker) and exposure to an air draft for 10 days (desiccation stress [DS] 10D group)...
2015: Molecular Vision
https://www.readbyqxmd.com/read/26348638/improvement-of-outcome-measures-of-dry-eye-by-a-novel-integrin-antagonist-in-the-murine-desiccating-stress-model
#18
Achim H Krauss, Rosa M Corrales, Flavia S A Pelegrino, Johanna Tukler-Henriksson, Stephen C Pflugfelder, Cintia S de Paiva
PURPOSE: We investigated the effects of GW559090, a novel, competitive, and high-affinity α4 integrin antagonist, in a murine model of dry eye. Through interaction with vascular cell adhesion molecule 1 (VCAM-1) and fibronectin α4β1 integrin is involved in leukocyte trafficking and activation. METHODS: Female C57BL/6 mice, aged 6 to 8 weeks, were subjected to desiccating stress (DS). Bilateral topical twice daily treatment with GW559090 was compared to vehicle-treated controls...
September 2015: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26225631/desiccating-stress-induced-mmp-production-and-activity-worsens-wound-healing-in-alkali-burned-corneas
#19
Fang Bian, Flavia S A Pelegrino, Stephen C Pflugfelder, Eugene A Volpe, De-Quan Li, Cintia S de Paiva
PURPOSE: To evaluate the effects of dry eye on ocular surface protease activity and sight threatening corneal complications following ocular surface chemical injury. METHODS: C57BL/6 mice were subjected to unilateral alkali burn (AB) with or without concomitant dry eye for 2 or 5 days. Mice were observed daily for appearance of corneal perforation. Whole corneas were harvested and lysed for RNA extraction. Quantitative real-time PCR was performed to measure expression of inflammation cytokines, matrix metalloproteinases (MMP)...
July 2015: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26203745/effects-of-quercetin-in-a-mouse-model-of-experimental-dry-eye
#20
Ha Na Oh, Chae Eun Kim, Ji Hyun Lee, Jae Wook Yang
PURPOSE: To evaluate the effect of treatment with quercetin in a mouse model of dry eye. METHODS: 0.5% quercetin eye drops were prepared and an experimental dry eye model was induced in NOD.B10.H2(b) mice through desiccation stress. The mice were divided into 3 groups according to the treatment regimen: the DS 10D group (desiccation stress for 10 days), the phosphate buffered saline (PBS) group, and the quercetin group. Tear volumes and corneal irregularity scores were measured at 3, 5, 7, and 10 days after treatment...
September 2015: Cornea
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