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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/29790699/evaluation-of-the-secretion-and-release-of-vascular-endothelial-growth-factor-from-two-dimensional-culture-and-three-dimensional-cell-spheroids-formed-with-stem-cells-and-osteoprecursor-cells
#1
Hyunjin Lee, Sung-Il Lee, Youngkyung Ko, Jun-Beom Park
BACKGROUND: Co-culture has been applied in cell therapy, including stem cells, and has been reported to give enhanced functionality. OBJECTIVES: In this study, stem-cell spheroids were formed in concave micromolds at different ratios of stem cells to osteoprecursor cells, and the amount of secretion of vascular endothelial growth factor (VEGF) was evaluated. MATERIAL AND METHODS: Gingiva-derived stem cells and osteoprecursor cells in the amount of 6 × 105 were seeded on a 24-well culture plate or concave micromolds...
May 18, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29782191/lipid-protein-and-microrna-composition-within-mesenchymal-stem-cell-derived-exosomes
#2
Hao Deng, Chun Sun, Yingxin Sun, Huhu Li, Lin Yang, Danbin Wu, Qing Gao, Xijuan Jiang
Mesenchymal stem cells (MSCs) were regarded as one of the most promising type of seed cells in tissue engineering due to its easy accessibility and multipotent feature of being able to differentiate into adipocyte, osteoblast, cardiomyocytes, and neurons. For years, MSCs have been applied in treating cardiovascular disease, reconstructing kidney injury, and remodeling immune system with remarkable achievements. Basic researches revealed that its clinic effects are not only due to their pluripotent ability but also through their paracrine function that they synthesize and secrete a broad spectrum of growth factors and cytokines...
May 21, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29780570/engineering-a-microcirculation-for-perfusion-control-of-ex-vivo-assembled-organ-systems-challenges-and-opportunities
#3
REVIEW
Pavan Kottamasu, Ira Herman
Donor organ shortage remains a clear problem for many end-stage organ patients around the world. The number of available donor organs pales in comparison with the number of patients in need of these organs. The field of tissue engineering proposes a plausible solution. Using stem cells, a patient's autologous cells, or allografted cells to seed-engineered scaffolds, tissue-engineered constructs can effectively supplement the donor pool and bypass other problems that arise when using donor organs, such as who receives the organ first and whether donor organ rejection may occur...
January 2018: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29775181/porous-lithium-doped-hydroxyapatite-scaffold-seeded-with-hypoxia-preconditioned-bone-marrow-mesenchymal-stem-cells-for-bone-tissue-regeneration
#4
Donghai Li, Huifang Liu, Jinhai Zhao, Zhouyuan Yang, Xiaowei Xie, Zhun Wei, Dongzhe Li, Pengde Kang
Hydroxyapatite (HA) is a commonly used biomaterial in bone-tissue engineering, but pure HA is deficient in osteoinduction. In this study, we fabricated scaffolds of lithium-doped HA (Li-HA) and assess the bone generation enhancement of Li-HA scaffolds seeded with hypoxia-preconditioned bone-marrow mesenchymal stem cells (BMMSCs). We found that 1.5%Li-HA obtained optimal cell proliferation activity in vitro. In an in vivo study, Li-HA/BMSCs enhanced new bone formation, reducing the GSK-3β and increasing the β-catenin, but the angiogenic effect was not modified significantly...
May 18, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29772539/spheroids-of-stem-cells-as-endochondral-templates-for-improved-bone-engineering
#5
Leandra Santos Baptista, Gabriela Soares Kronemberger, Karina Ribeiro Silva, Jose Mauro Granjeiro
Osteodegenerative disease and bone fractures lead to bone damage or loss, requiring new bone formation to replace the damaged tissues. Classical 'top-down' tissue engineering relies on seeding cell suspensions into biomaterial scaffolds, and then guiding cell fate by growth factors. However, complex tissue fabrication using this approach has important limitations. 'Bottom-up' tissue engineering has the potential to overcome the drawbacks of the top-down approach, by using 'building blocks' of cell spheroids for tissue biofabrication without a scaffold...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29772189/comparison-of-intraoral-bone-regeneration-with-iliac-and-alveolar-bmscs
#6
F Wang, Y Zhou, J Zhou, M Xu, W Zheng, W Huang, W Zhou, Y Shen, K Zhao, Y Wu, D Zou
This study compared the osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) of iliac and alveolar origins (I-BMSCs and Al-BMSCs, respectively), which were transplanted in combination with β tricalcium phosphate (β-TCP) in peri-implant bone defects to investigate the osseointegration between dental implants and tissue-engineered bone in dogs. Specifically, I-BMSCs and Al-BMSCs were cultured, characterized, and seeded on β-TCP and subjected to immunoblotting analyses and alkaline phosphatase activity assays...
May 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29766664/tissue-engineered-nigrostriatal-pathway-for-treatment-of-parkinson-s-disease
#7
Laura A Struzyna, Kevin D Browne, Zachary D Brodnik, Justin C Burrell, James P Harris, H Isaac Chen, John A Wolf, Kate V Panzer, James Lim, John E Duda, Rodrigo A España, D Kacy Cullen
The classic motor deficits of Parkinson's disease are caused by degeneration of dopaminergic neurons in the substantia nigra pars compacta, resulting in the loss of their long-distance axonal projections that modulate the striatum. Current treatments only minimize the symptoms of this disconnection as there is no approach capable of replacing the nigrostriatal pathway. We are applying micro-tissue engineering techniques to create living, implantable constructs that mimic the architecture and function of the nigrostriatal pathway...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29763345/tissue-engineering-of-necrotic-dental-pulp-of-immature-teeth-with-apical-periodontitis-in-dogs-radiographic-and-histological-evaluation
#8
Eman A El Ashiry, Najlaa M Alamoudi, Mahmoud K El Ashiry, Hagar A Bastawy, Douaa A El Derwi, Hazem M Atta
AIM: To evaluate tissue engineering technology to regenerate pulp-dentin like tissues in pulp canals of immature necrotic permanent teeth with apical periodontitis in dogs. STUDY DESIGN: The study was performed on 36 teeth in 12 dogs. The experiment was carried out using split mouth design. In each dog 3 teeth were selected for implementing the study procedure. Apical periodontitis was induced in Group A and B teeth. Group (A): immature upper left 2nd permanent incisors that were transplanted with a construct of autologous dental pulp stem cells with growth factors seeded in a chitosn hydrogel scaffold...
May 15, 2018: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/29751848/differentiation-of-adipose-derived-stem-cells-into-schwann-cell-like-cells-through-intermittent-induction-potential-advantage-of-cellular-transient-memory-function
#9
Xun Sun, Yun Zhu, He-Yong Yin, Zhi-Yuan Guo, Feng Xu, Bo Xiao, Wen-Li Jiang, Wei-Min Guo, Hao-Ye Meng, Shi-Bi Lu, Yu Wang, Jiang Peng
BACKGROUND: Peripheral nerve injury (PNI) is a worldwide issue associated with severe social and economic burden. Autologous nerve grafting, the gold standard treatment for peripheral nerve defects, still has a number of technical limitations. Tissue engineering technology is a novel therapeutic strategy, and mesenchymal stromal cells (MSCs) are promising seed cells for nerve tissue engineering. However, the efficiency of traditional methods for inducing the differentiation of MSCs to Schwann cell-like cells (SCLCs) remains unsatisfactory...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29745614/-the-application-of-nanotopography-in-the-development-of-tissue-engineered-tissues-using-mesenchymal-stem-cells
#10
REVIEW
Weiying Fang, Tingyu Gu, Yi Liu, Lan Xu, Yuelei Chen
Tissue engineering has emerged as a promising approach for the repair and functional reconstruction of damaged tissues. The bionic and intelligentized scaffolds provide the structural support for cell growth and differentiation as well as tissue regeneration. The surface properties of the biological material implant, the nanotopology in particular, become key aspects in determining the success of the implant. Mesenchymal stem cells (MSC) are widely favored by researchers as the seed cells in tissue engineering...
February 1, 2018: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29744423/3d-additive-manufactured-nanocomposite-magnetic-scaffolds-effect-of-the-application-mode-of-a-time-dependent-magnetic-field-on-hmscs-behavior
#11
Ugo D'Amora, Teresa Russo, Antonio Gloria, Virginia Rivieccio, Vincenzo D'Antò, Giacomo Negri, Luigi Ambrosio, Roberto De Santis
Over the past few years, the influence of static or dynamic magnetic fields on biological systems has become a topic of considerable interest. Magnetism has recently been implicated to play significant roles in the regulation of cell responses and, for this reason, it is revolutionizing many aspects of healthcare, also suggesting new opportunities in tissue engineering. The aim of the present study was to analyze the effect of the application mode of a time-dependent magnetic field on the behavior of human mesenchymal stem cells (hMSCs) seeded on 3D additive-manufactured poly(ɛ-caprolactone)/iron-doped hydroxyapatite (PCL/FeHA) nanocomposite scaffolds...
September 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29744414/human-adipose-derived-stem-cells-are-superior-to-human-osteoblasts-hob-in-bone-tissue-engineering-on-a-collagen-fibroin-elr-blend
#12
Esen Sayin, Rosti Hama Rashid, José Carlos Rodríguez-Cabello, Ahmed Elsheikh, Erkan Türker Baran, Vasif Hasirci
The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen - silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films...
June 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29741358/fibrous-topography-potentiated-canonical-wnt-signaling-directs-the-odontoblastic-differentiation-of-dental-pulp-derived-stem-cells
#13
Saeed Ur Rahman, Joung-Hwan Oh, Young-Dan Cho, Shin Hye Chung, Gene Lee, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms which underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29734041/attachment-and-spatial-organisation-of-human-mesenchymal-stem-cells-on-poly-ethylene-glycol-hydrogels
#14
Aman S Chahal, Manuel Schweikle, Catherine A Heyward, Hanna Tiainen
Strategies that enable hydrogel substrates to support cell attachment typically incorporate either entire extracellular matrix proteins or synthetic peptide fragments such as the RGD (arginine-glycine-aspartic acid) motif. Previous studies have carefully analysed how material characteristics can affect single cell morphologies. However, the influence of substrate stiffness and ligand presentation on the spatial organisation of human mesenchymal stem cells (hMSCs) have not yet been examined. In this study, we assessed how hMSCs organise themselves on soft (E = 7...
May 3, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29724151/physical-microenvironment-based-inducible-scaffold-for-stem-cell-tenogenesis-and-tendon-regeneration
#15
Hong Zhang, Meng-Fei Liu, Ri-Chun Liu, Wei-Liang Shen, Zi Yin, Xiao Chen
Tendon injuries are common musculoskeletal system disorders, but the tendons have poor regeneration ability. To address this issue, tendon tissue engineering provides potential strategies for future therapeutic treatment. Elements of the physical microenvironment, such as the mechanical force and surface topography, play a vital role in regulating stem cell fate, enhancing the differentiation efficiency of seed cells in tendon tissue engineering. Various inducible scaffolds have been widely explored for tendon regeneration, and scaffold-enhancing modifications have been extensively studied...
May 4, 2018: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29717817/a-multilayer-scaffold-design-with-spatial-arrangement-of-cells-to-modulate-esophageal-tissue-growth
#16
Sherif Soliman, Julie Laurent, Lena Kalenjian, Kalia Burnette, Bert Hedberg, Saverio La Francesca
Esophageal diseases may require resectioning of the damaged portion. The current standard of care requires the replacement of the esophagus with the stomach or the intestine. Such procedures have high rates of mortality and morbidity; therefore, the use of alternative conduits is needed. A tissue engineering approach that allows for the regeneration of esophageal tissues would have significant clinical application. A cell-seeded synthetic scaffold could replace the resected part of the esophagus and elicit tissue regrowth...
May 2, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29717628/adipose-derived-stem-cell-implanted-poly-%C3%AF%C2%B5-caprolactone-chitosan-scaffold-improves-bladder-regeneration-in-a-rat-model
#17
Zhe Zhou, Hao Yan, Yidong Liu, Dongdong Xiao, Wei Li, Qiong Wang, Yang Zhao, Kang Sun, Ming Zhang, Mujun Lu
AIM: The study investigated the feasibility of seeding adipose-derived stem cells (ASCs) onto a poly(ϵ-caprolactone)/chitosan (PCL/CS) scaffold for bladder reconstruction using a rat model of bladder augmentation. MATERIALS & METHODS: In the experimental group, the autologous ASCs were seeded onto the PCL/CS scaffold for bladder augmentation. An unseeded scaffold was used for bladder augmentation as control group. The sham group was also set. RESULT: 8 weeks after implantation, more densely smooth muscles were detected in the experimental group with a larger bladder capacity and more intensive blood vessels...
April 2018: Regenerative Medicine
https://www.readbyqxmd.com/read/29717624/chondrogenic-differentiation-processes-in-human-bone-marrow-aspirates-seeded-in-three-dimensional-woven-poly-%C3%AE%C2%B5-caprolactone-scaffolds-enhanced-by-raav-mediated-sox9-gene-transfer
#18
Jagadeesh Kumar Venkatesan, Franklin T Moutos, Ana Rey-Rico, Bradley T Estes, Janina Frisch, Gertrud Schmitt, Henning Madry, Farshid Guilak, Magali Cucchiarini
Combining gene therapy approaches with tissue engineering procedures is an active area of translational research for the effective treatment of articular cartilage lesions, especially to target chondrogenic progenitor cells such as those derived from the bone marrow. Here, we evaluated the effect of genetically modifying concentrated human mesenchymal stem cells from bone marrow to induce chondrogenesis by recombinant adeno-associated viral (rAAV) vector gene transfer of the sex-determining region Y-type high-mobility group box 9 (SOX9) factor upon seeding in three-dimensional (3D) woven poly(ε-caprolactone) (PCL) scaffolds that provide mechanical properties mimicking those of native articular cartilage...
May 2, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29717356/mesenchymal-stem-cells-seeded-onto-tissue-engineered-osteoinductive-scaffolds-enhance-the-healing-process-of-critical-sized-radial-bone-defects-in-rat
#19
Ahmad Oryan, Mohamadreza Baghaban Eslaminejad, Amir Kamali, Samaneh Hosseini, Ali Moshiri, Hossein Baharvand
Long bone defects comprise one of the most prevalent clinical problems worldwide and the current bone grafting materials have major limitations to repair them. Although tremendous efforts have been made to repair critical-sized long bone defects in animal models, designing an optimal bone tissue-engineered substitute remains one of the main challenges. Hence, this study aims to closely mimic a natural bone healing process by a tissue-engineered construct including osteoinductive materials pre-seeded with bone marrow-derived mesenchymal stem cells (BMSCs)...
May 1, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29707747/comparison-of-human-denuded-amniotic-membrane-and-porcine-small-intestine-submucosa-as-scaffolds-for-limbal-mesenchymal-stem-cells
#20
Liliana I Sous Naasani, Cristiano Rodrigues, Jéssica Gonçalves Azevedo, Aline F Damo Souza, Silvio Buchner, Márcia R Wink
Blinding corneal scarring is usually treated with allogeneic graft tissue. Nevertheless, the global shortage of donors leaves millions of patients in need of therapy. Traditional tissue engineering strategies involves the combination of cells, growth factors, and scaffolds that can supply cellular biological components allowing to restore the tissue function. The mesenchymal stem cells found in the limbal stroma (L-MSCs) have a self-renewal potential for multilineage differentiation. Thus, in this work we compared the potential of human amniotic membrane (hAM) and porcine small intestine submucosa (SIS) as scaffolds for L-MSCs, aiming at potential applications in corneal regeneration...
April 29, 2018: Stem Cell Reviews
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