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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/29137121/human-mesenchymal-stem-cells-growth-and-osteogenic-differentiation-on-piezoelectric-poly-vinylidene-fluoride-microsphere-substrates
#1
R Sobreiro-Almeida, M N Tamaño-Machiavello, E O Carvalho, L Cordón, S Doria, L Senent, D M Correia, C Ribeiro, S Lanceros-Méndez, R Sabater I Serra, J L Gomez Ribelles, A Sempere
The aim of this work was to determine the influence of the biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture supports were prepared with structures ranging between 2D and 3D, based on PVDF films on which PVDF microspheres were deposited with varying surface density. Maintenance of multipotentiality when cultured in expansion medium was studied by flow cytometry monitoring the expression of characteristic hMSCs markers, and revealed that cells were losing their characteristic surface markers on these supports...
November 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#2
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134814/direct-uv-triggered-thiol-ene-cross-linking-of-electrospun-polyester-fibers-from-unsaturated-poly-macrolactone-s-and-their-drug-loading-by-solvent-swelling
#3
Fernando C S de Oliveira, Dinorath Olvera, Michael J Sawkins, Sally-Ann Cryan, Scott D Kimmins, Tatiane Eufrasio da Silva, Daniel J Kelly, Garry P Duffy, Cathal Kearney, Andreas Heise
Electrospinning is considered a relatively simple and versatile technique to form high porosity porous scaffolds with micron to nanoscale fibers for biomedical applications. Here, electrospinning of unsaturated aliphatic polyglobalide (PGl) into well-defined fibers with an average diameter of 9 μm is demonstrated. Addition of a dithiol cross-linker and a photoinitiator to the polymer solution enabled the UV-triggered intracross-linking of the fibers during the spinning process. The in situ cross-linking of the fibers resulted in amorphous material able to swell up to 14% in tetrahydrofurane (THF) without losing the fiber morphology...
November 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#4
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29131080/complete-static-repopulation-of-decellularized-porcine-tissues-for-heart-valve-engineering-an-in-vitro-study
#5
Annelies Roosens, Mahtab Asadian, Nathalie De Geyter, Pamela Somers, Ria Cornelissen
To date, a completely in vitro repopulated tissue-engineered heart valve has not been developed. This study focused on sequentially seeding 2 cell populations onto porcine decellularized heart valve leaflets (HVL) and pericardia (PER) to obtain fully repopulated tissues. For repopulation of the interstitium, porcine valvular interstitial cells (VIC) and bone marrow-derived mesenchymal stem cells (BM-MSC) or adipose tissue-derived stem cells (ADSC) were used. In parallel, the culture medium was supplemented with ascorbic acid 2-phosphate (AA) and its effect on recolonization was investigated...
November 8, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29128540/electrospun-thermosensitive-hydrogel-scaffold-for-enhanced-chondrogenesis-of-human-mesenchymal-stem-cells
#6
Alexander R Brunelle, Christopher B Horner, Karen Low, Gerardo Ico, Jin Nam
Hydrogels have shown great potential for cartilage tissue engineering applications due to their capability to encapsulate cells within biomimetic, 3-dimensional (3D) microenvironments. However, the multi-step fabrication process that is necessary to produce cell/scaffold constructs with defined dimensions, limits their off-the-shelf translational usage. In this study, we have developed a hybrid scaffolding system which combines a thermosensitive hydrogel, poly(ethylene glycol)-poly(N-isopropylacrylamide) (PEG-PNIPAAm), with a biodegradable polymer, poly(ε-caprolactone) (PCL), into a composite, electrospun microfibrous structure...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29128533/fabrication-of-dense-anisotropic-collagen-scaffolds-using-biaxial-compression
#7
Jared L Zitnay, Shawn P Reese, Garvin Tran, Niloofar Farhang, Robert D Bowles, Jeffrey A Weiss
We developed a new method to manufacture dense, aligned, and porous collagen scaffolds using biaxial plastic compression of type I collagen gels. Using a novel compression apparatus that constricts like an iris diaphragm, low density collagen gels were compressed to yield a permanently densified, highly aligned collagen material. Micro-porosity scaffolds was created using hydrophilic elastomer porogens that can be selectively removed following biaxial compression, with porosity modulated by using different porogen concentrations...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29123550/mesenchymal-stem-cells-for-cartilage-regeneration-of-tmj-osteoarthritis
#8
REVIEW
Dixin Cui, Hongyu Li, Xin Xu, Ling Ye, Xuedong Zhou, Liwei Zheng, Yachuan Zhou
Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and structure-modifying effects to restore impaired cartilage as well as other TMJ tissues. In recent years, stem cell-based therapy has raised much attention as an alternative approach towards tissue repair and regeneration...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29119734/tissue-engineered-composite-scaffold-of-poly-lactide-co-glycolide-and-hydroxyapatite-nanoparticles-seeded-with-autologous-mesenchymal-stem-cells-for-bone-regeneration
#9
Bing Zhang, Pei-Biao Zhang, Zong-Liang Wang, Zhong-Wen Lyu, Han Wu
OBJECTIVE: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/ poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. METHODS: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29115567/preparation-and-characterization-of-acellular-adipose-tissue-matrix-using-a-combination-of-physical-and-chemical-treatments
#10
Mei Song, Yi Liu, Ling Hui
Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues. The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material. The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion...
October 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29107590/human-pluripotent-stem-cell-derived-cardiac-tissue-like-constructs-for-repairing-the-infarcted-myocardium
#11
Junjun Li, Itsunari Minami, Motoko Shiozaki, Leqian Yu, Shin Yajima, Shigeru Miyagawa, Yuji Shiba, Nobuhiro Morone, Satsuki Fukushima, Momoko Yoshioka, Sisi Li, Jing Qiao, Xin Li, Lin Wang, Hidetoshi Kotera, Norio Nakatsuji, Yoshiki Sawa, Yong Chen, Li Liu
High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We show that multilayered and elongated CMs could be organized at high density along aligned nanofibers in a simple one-step seeding process, resulting in upregulated cardiac biomarkers and enhanced cardiac functions...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29106428/modified-acellular-nerve-delivering-pmscs-improve-functional-recovery-in-rats-after-complete-spinal-cord-transection
#12
Ting Tian, Zhenhai Yu, Naili Zhang, Yingwei Chang, Yuqiang Zhang, Luping Zhang, Shuai Zhou, Chunlei Zhang, Guoying Feng, Fei Huang
Due to the poor regeneration capacity of neurons and the inhibitory microenvironment, spontaneous regeneration in spinal cord injury (SCI) remains challenging. Tissue engineering is considered a promising approach for enhancing the regeneration of SCI by reconstructing the inherent structure and improving the microenvironment. In this study, the possibility of engineering a nerve complex, which is constructed by acellular nerve delivering placenta mesenchymal stem cells (PMSCs), was assessed for the recovery of a transected spinal cord...
November 6, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/29106037/mesenchymal-stem-cells-seeded-on-a-human-amniotic-membrane-improve-liver-regeneration-and-mouse-survival-after-extended-hepatectomy
#13
Aure Despeyroux, Cédric Duret, Claire Gondeau, Esther Perez-Gracia, Lisa Chuttoo, Hugues de Boussac, Philippe Briolotti, Claire Bony, Danièle Noël, Christian Jorgensen, Dominique Larrey, Martine Daujat-Chavanieu, Astrid Herrero
Liver failure remains the leading cause of postoperative mortality after hepatectomy. The present study investigated the effect of treatment with allogenic mesenchymal stem cells (MSCs) on survival and liver regeneration 48h and 7 days after 80% hepatectomy in C57Bl/6 mice. To optimize their biodistribution, MSCs were grown on acellular human amniotic membranes (HAM) and applied as a patch on the remnant liver. This approach was compared with MSC infusion and HAM patch alone. Hepatectomized mice without any treatment were used as control group...
November 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29103309/the-effect-of-nanofibre-based-polyethersulfone-pes-scaffold-on-the-chondrogenesis-of-human-induced-pluripotent-stem-cells
#14
Hossein Mahboudi, Masoud Soleimani, Seyed Ehsan Enderami, Mousa Kehtari, Hana Hanaee-Ahvaz, Hossein Ghanbarian, Mojgan Bandehpour, Shahrzad Nojehdehi Nojehdehi Sh, Samaneh Mirzaei, Bahram Kazemi
Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue engineering. In this study, we considered the effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of iPSCs. IPSC cells were cultured on the PES scaffold and scaffold free method. After 21 d, real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels...
November 6, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29102342/bioengineered-lungs-a-challenge-and-an-opportunity
#15
Ramon Farré, Jordi Otero, Isaac Almendros, Daniel Navajas
Lung biofabrication is a new tissue engineering and regenerative development aimed at providing organs for potential use in transplantation. Lung biofabrication is based on seeding cells into an acellular organ scaffold and on culturing them in an especial purpose bioreactor. The acellular lung scaffold is obtained by decellularizing a non-transplantable donor lung by means of conventional procedures based on application of physical, enzymatic and detergent agents. To avoid immune recipient's rejection of the transplanted bioengineered lung, autologous bone marrow/adipose tissue-derived mesenchymal stem cells, lung progenitor cells or induced pluripotent stem cells are used for biofabricating the bioengineered lung...
November 1, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29083361/chondral-tissue-engineering-of-the-reumatoid-knee-with-collagen-matrix-autologous-chondrocytes-implant
#16
Massimiliano Leigheb, Michela Bosetti, Antonio De Consoli, Alessia Borroni, Mario Cannas, Federico Grassi
Articular cartilage repair is still a challenge. To date evidence is insufficient to support a treatment over the others. Inflammatory conditions in the joint hamper the application of tissue engineering during chronic joint diseases. Most of the Matrix Autologous Chondrocyte Implantation (MACI) cases reported in literature do not deal with rheumatoid knees and do not have a long clinical-histologic follow-up. We report about a 46-year old woman who suffered of a painful focal Outerbridge 4th degree chondral lesion in the medial femoral condyle of her left rheumatoid knee...
October 18, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/29082354/biophysical-regulation-of-cancer-stem-initiating-cells-implications-for-disease-mechanisms-and-translation
#17
Joseph Chen, Sanjay Kumar
Cancer stem/initiating cells (CSCs) are a subset of tumor cells proposed to play privileged roles in seeding tumors and driving metastasis. CSCs have emerged as an increasingly important target of interest in cancer biology and therapy. Recent work has suggested that CSC maintenance and metastatic potential may be modulated by physical inputs within the tissue microenvironment, including interstitial pressure and extracellular matrix stiffness. Here we review recent progress in our understanding of CSC regulation by biophysical signals within the tumor microenvironment...
March 2017: Curr Opin Biomed Eng
https://www.readbyqxmd.com/read/29073831/pthrp-gradients-affect-the-progression-of-mesenchymal-stem-cell-chondrogenesis-and-hypertrophy
#18
Niamh Fahy, Oliver Gardner, Mauro Alini, Martin James Stoddart
INTRODUCTION: Mesenchymal stem cells (MSCs) are considered a promising cell source for cartilage repair strategies due to their chondrogenic differentiation potential. However, their in-vitro tendency to progress towards hypertrophy limits their clinical use. This unfavourable result may be due to the fact that MSCs used in tissue engineering approaches are all at the same developmental stage, and have lost crucial spatial and temporal signalling cues. In this study, we sought to investigate the effect of a spatial parathyroid hormone-related protein (PTHrP) signalling gradient on the chondrogenic differentiation of MSCs and progression to hypertrophy...
October 26, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29069818/inhibiting-phd2-in-bone-marrow-mesenchymal-stem-cells-via-lentiviral-vector-mediated-rna-interference-facilitates-the-repair-of-periodontal-tissue-defects-in-sd-rats
#19
Changxing Chen, Houxuan Li, Jun Jiang, Qian Zhang, Fuhua Yan
Hypoxia-inducible factors (HIFs) play an important role in angiogenesis, and they can activate the expression of several downstream angiogenic factors. HIF-1 is a major transcriptor of HIFs, composed of α and β subunits. Prolyl hydroxylase domain-containing protein 2 (PHD2) is the main catabolic enzyme for HIF-1α, and it can accelerate its degradation under normoxic conditions. PHD2 expression in bone marrow mesenchymal stem cells (BMMSCs) of SD rats was down-regulated under normoxic conditions in this study by utilizing lentiviral vector-mediated RNA interference to promote HIF-1α accumulation, thus enhancing the expression of angiogenic factors...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29064570/effects-of-scaffold-microstructure-and-low-intensity-pulsed-ultrasound-on-chondrogenic-differentiation-of-human-mesenchymal-stem-cells
#20
Mitra Aliabouzar, Se-Jun Lee, Xuan Zhou, Grace Lijjie Zhang, Kausik Sarkar
The effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) scaffolds with varying pore geometries (square and hexagonal channels) were investigated. The scaffold with square pores resulted in higher hMSC growth and chondrogenic differentiation than a solid or a hexagonally porous scaffold. The optimal LIPUS parameters at 1.5 MHz were found to be 100 mW/cm(2) and 20% duty cycle...
October 24, 2017: Biotechnology and Bioengineering
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