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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/27906085/tissue-engineered-cardiac-patch-seeded-with-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-promoted-the-regeneration-of-host-cardiomyocytes-in-a-rat-model
#1
Tadahisa Sugiura, Narutoshi Hibino, Christopher K Breuer, Toshiharu Shinoka
BACKGROUND: Thousands of babies are born with congenital heart defects that require surgical repair involving a prosthetic implant. Lack of growth in prosthetic grafts is especially detrimental in pediatric surgery. Cell seeded biodegradable tissue engineered grafts are a novel solution to this problem. The purpose of the present study is to evaluate the feasibility of seeding human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) onto a biodegradable cardiac patch. METHODS: The hiPS-CMs were cultured on a biodegradable patch composed of a polyglycolic acid (PGA) and a 50:50 poly (l-lactic-co-ε-caprolactone) copolymer (PLCL) for 1 week...
December 1, 2016: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#2
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27876676/anatomical-region-dependent-enhancement-of-3-dimensional-chondrogenic-differentiation-of-human-mesenchymal-stem-cells-by-soluble-meniscus-extracellular-matrix
#3
Benjamin B Rothrauff, Kazunori Shimomura, Riccardo Gottardi, Peter G Alexander, Rocky S Tuan
: Extracellular matrix (ECM) derived from decellularized tissues has been found to promote tissue neogenesis, most likely mediated by specific biochemical and physical signaling motifs that promote tissue-specific differentiation of progenitor cells. Decellularized ECM has been suggested to be efficacious for the repair of tissue injuries. However, decellularized meniscus contains a dense collagenous structure, which impedes cell seeding and infiltration and is not readily applicable for meniscus repair...
November 19, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27832002/renal-differentiation-of-mesenchymal-stem-cells-seeded-on-nanofibrous-scaffolds-improved-by-human-renal-tubular-cell-lines-conditioned-medium
#4
Abdolreza Ardeshirylajimi, Saeid Vakilian, Mohammad Salehi
Kidney injuries and renal dysfunctions are one of the most important clinical problems and tissue engineering could be a valuable method for solving it. The objective of this study was to investigate the synergistic effect of renal cell line conditioned medium and Polycaprolactone nanofibers on renal differentiation of human mesenchymal stem cells. In the present study, after stem cells isolation and characterization, Polycaprolactone nanofibrous scaffold was fabricated using electrospinning methods and characterized morphologically, mechanically and biocompatibility...
November 9, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/27830445/tissue-engineering-for-neurodegenerative-diseases-using-human-amniotic-membrane-and-umbilical-cord
#5
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
November 9, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27825527/sensing-metabolites-for-the-monitoring-of-tissue-engineered-construct-cellularity-in-perfusion-bioreactors
#6
Aaron D Simmons, Cortes Williams, Antoine Degoix, Vassilios I Sikavitsas
As the field of tissue engineering progresses ever-further toward realizing clinical implementation of tissue-engineered constructs for wound regeneration, perhaps the most significant hurdle remains the establishment of non-destructive means for real-time in vitro assessment. In order to address this barrier, the study presented herein established the viability of the development of correlations between metabolic rates (specifically oxygen uptake, glucose consumption, and lactate production) and the cellularity of tissue-engineered cultures comprised of rat mesenchymal stem cells dynamically seeded on 85% porous nonwoven spunbonded poly(l-lactic acid) fiber mesh scaffolds...
September 30, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27821864/chondrogenic-potential-of-peripheral-blood-derived-mesenchymal-stem-cells-seeded-on-demineralized-cancellous-bone-scaffolds
#7
Shao-Jie Wang, Dong Jiang, Zheng-Zheng Zhang, Ai-Bing Huang, Yan-Song Qi, Hai-Jun Wang, Ji-Ying Zhang, Jia-Kuo Yu
As a cell source with large quantity and easy access, peripheral blood mesenchymal stem cells (PBMSCs) were isolated and seeded in porcine demineralized cancellous bone (DCB) scaffolds, cultured in chondrogenic medium and evaluated for in vitro chondrogenesis. Bone marrow MSCs (BMMSCs) and articular cartilage chondrocytes (ACCs) underwent the same process as controls. The morphology, viability and proliferation of PBMSCs in DCB scaffolds were similar to those of BMMSCs and ACCs. PBMSCs and BMMSCs showed similar chondrogenesis potential with consistent production of COL 2 and SOX 9 protein and increased COL 2 and AGC mRNA expressions at week 3 but the COL 2 protein production was still less than that of ACCs...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27811845/dental-pulp-stem-cells-express-tendon-markers-under-mechanical-loading-and-are-a-potential-cell-source-for-tissue-engineering-of-tendon-like-tissue
#8
Yu-Ying Chen, Sheng-Teng He, Fu-Hua Yan, Peng-Fei Zhou, Kai Luo, Yan-Ding Zhang, Yin Xiao, Min-Kui Lin
Postnatal mesenchymal stem cells have the capacity to differentiate into multiple cell lineages. This study explored the possibility of dental pulp stem cells (DPSCs) for potential application in tendon tissue engineering. The expression of tendon-related markers such as scleraxis, tenascin-C, tenomodulin, eye absent homologue 2, collagens I and VI was detected in dental pulp tissue. Interestingly, under mechanical stimulation, these tendon-related markers were significantly enhanced when DPSCs were seeded in aligned polyglycolic acid (PGA) fibre scaffolds...
November 4, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27809678/tendon-derived-extracellular-matrix-enhances-tgf-%C3%AE-3-induced-tenogenic-differentiation-of-human-adipose-derived-stem-cells
#9
Guang Yang, Benjamin B Rothrauff, Hang Lin, Shuting Yu, Rocky Tuan
Because of the limited and unsatisfactory outcomes of clinical tendon repair, tissue engineering approaches using adult mesenchymal stem cells (MSCs) are being considered as a promising alternative strategy to heal tendon injuries. Successful and functional tendon tissue engineering depends on harnessing the biochemical cues presented by the native tendon extracellular matrix (ECM) and the embedded tissue-specific bio-factors. In this study, we have prepared and characterized the biological activities of a soluble extract of decellularized tendon ECM (tECM) on adult adipose derived stem cells (ASCs), on the basis of histological, biochemical, and gene expression analyses...
November 3, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27808102/bioinspired-seeding-of-biomaterials-using-three-dimensional-microtissues-induces-chondrogenic-stem-cell-differentiation-and-cartilage-formation-under-growth-factor-free-conditions
#10
J Leijten, L S Moreira Teixeira, J Bolander, W Ji, B Vanspauwen, J Lammertyn, J Schrooten, F P Luyten
Cell laden biomaterials are archetypically seeded with individual cells and steered into the desired behavior using exogenous stimuli to control growth and differentiation. In contrast, direct cell-cell contact is instructive and even essential for natural tissue formation. Namely, microaggregation and condensation of mesenchymal progenitor cells triggers chondrogenesis and thereby drives limb formation. Yet a biomimetic strategy translating this approach into a cell laden biomaterial-based therapy has remained largely unexplored...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27806676/the-role-of-cell-seeding-bio-scaffolds-and-the-in-vivo-microenvironment-in-the-guided-generation-of-osteochondral-composite-tissue
#11
Jiao Wei, Tanja Herrler, Kai Liu, Dong Han, Mei Yang, Chuanchang Dai, Qing Feng Li
Tissue engineering based on cell seeding, bio-scaffolds, and growth factors has been widely applied for the reconstruction of tissue defects. Recent progress has fueled in vivo tissue engineering techniques in becoming hot topics in regenerative medicine and reconstructive surgery. To improve the efficacy of tissue engineering, we here investigated the roles of cell seeding, bio-scaffolds, growth factors, and in-vivo microenvironment in tissue regeneration. Bone marrow derived stem cells (BMSCs), allogenic demineralized bone matrix (DBM) as bio-scaffold, and growth factors bone morphogenetic protein 2/ transforming growth factor (BMP-2/TGF-β1), and the in-vivo microenvironment of rib periosteum and perichondrium were used in different combinations for the generation of osteochondral composite tissue...
November 2, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27785631/odontogenic-differentiation-and-biomineralization-potential-of-dental-pulp-stem-cells-inside-mg-based-bioceramic-scaffolds-under-low-level-laser-treatment
#12
Anna Theocharidou, Athina Bakopoulou, Eleana Kontonasaki, Eleni Papachristou, Christina Hadjichristou, Maria Bousnaki, George Theodorou, Lambrini Papadopoulou, Nikolaos Kantiranis, Konstantinos Paraskevopoulos, Petros Koidis
This study aimed to investigate the potential of low-level laser irradiation (LLLI) to promote odontogenic differentiation and biomineralization by dental pulp stem cells (DPSCs) seeded inside bioceramic scaffolds. Mg-based, Zn-doped bioceramic scaffolds, synthesized by the sol-gel technique, were spotted with DPSCs and exposed to LLLI at 660 nm with maximum output power of 140 mw at fluencies (a) 2 and 4 J/cm(2) to evaluate cell viability/proliferation by the MTT assay and (b) 4 J/cm(2) to evaluate cell differentiation, using real-time PCR (expression of odontogenic markers) and a p-nitrophenylphosphate (pNPP)-based assay for alkaline phosphatase (ALP) activity measurement...
October 26, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27783699/association-of-17-%C3%AE-estradiol-with-adipose-derived-stem-cells-new-strategy-to-produce-functional-myogenic-differentiated-cells-with-a-nano-scaffold-for-tissue-engineering
#13
Chunxiang Feng, Jinqian Hu, Chang Liu, Shiliang Liu, Guiying Liao, Linjie Song, Xiaoyong Zeng
The increased incidence of stress urinary incontinence (SUI) in postmenopausal women has been proposed to be associated with a reduction in the level of 17-β estradiol (E2). E2 has also been shown to enhance the multi-differentiation ability of adipose-derived stem cells (ASCs) in vitro. However, studies on the potential value of E2 for tissue engineering in SUI treatment are rare. In the present study, we successfully fabricated myogenically differentiated ASCs (MD-ASCs), which were seeded onto a Poly(l-lactide)/Poly(e-caprolactone) electrospinning nano-scaffold, and incorporated E2 into the system, with the aim of improving the proliferation and myogenic differentiation of ASCs...
2016: PloS One
https://www.readbyqxmd.com/read/27778466/a-double-chamber-rotating-bioreactor-for-enhanced-tubular-tissue-generation-from-human-mesenchymal-stem-cells
#14
I Stefani, M A Asnaghi, J J Cooper-White, S Mantero
Cardiovascular diseases represent a major global health burden, with high rates of mortality and morbidity. Autologous grafts are commonly used to replace damaged or failing blood vessels, however such approaches are hampered by the scarcity of suitable graft tissue, donor site morbidity and poor long-term stability. Tissue engineering has been investigated as a means by which exogenous vessel grafts can be produced, with varying levels of success to date, a result of mismatched mechanical properties of these vessel substitutes and inadequate ex vivo vessel tissue genesis...
October 24, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27774108/a-controlled-release-codelivery-system-of-mscs-encapsulated-in-dextran-gelatin-hydrogel-with-tgf-%C3%AE-3-loaded-nanoparticles-for-nucleus-pulposus-regeneration
#15
Yibo Gan, Sukai Li, Pei Li, Yuan Xu, Liyuan Wang, Chen Zhao, Bin Ouyang, Bing Tu, Chengmin Zhang, Lei Luo, Xiangdong Luo, Xiumei Mo, Qiang Zhou
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP. The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27772730/regulation-of-the-secretion-of-immunoregulatory-factors-of-mesenchymal-stem-cells-mscs-by-collagen-based-scaffolds-during-chondrogenesis
#16
Jingyu Yang, Xuening Chen, Tun Yuan, Xiao Yang, Yujiang Fan, Xingdong Zhang
In the latest decade, mesenchymal stem cells (MSCs) have wildly considered as a source of seeded cells in tissue engineering, not only because of its multi-differentiation potentials, but also due to its immunoregulation ability. The main immunoregulatory features of MSCs could be divided into low self-immunogenicity and secretion of soluble factors. In this study, we explored how scaffold structures modulated the secretion of soluble immunoregulatory factors in MSCs under an allogeneic cartilage tissue engineering background...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27772709/electrospinning-of-poly-glycerol-sebacate-based-nanofibers-for-nerve-tissue-engineering
#17
Jue Hu, Dan Kai, Hongye Ye, Lingling Tian, Xin Ding, Seeram Ramakrishna, Xian Jun Loh
Nerve tissue engineering (TE) requires biomimetic scaffolds providing essential chemical and topographical cues for nerve regeneration. Poly(glycerol sebacate) (PGS) is a biodegradable and elastic polymer that has gained great interest as a TE scaffolding biomaterial. However, uncured PGS is difficult to be electrospun into nanofibers. PGS would, therefore, require the addition of electrospinning agents. In this study, we modified PGS by using atom transfer radical polymerization (ATRP) to synthesize PGS-based copolymers with methyl methacrylate (MMA)...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27770937/evaluation-of-the-potential-of-rhtgf-%C3%AE-3-encapsulated-p-lla-cl-collagen-nanofibers-for-tracheal-cartilage-regeneration-using-mesenchymal-stems-cells-derived-from-wharton-s-jelly-of-human-umbilical-cord
#18
Jing Wang, Binbin Sun, Lingling Tian, Xiaomin He, Qiang Gao, Tong Wu, Seeram Ramakrishna, Jinghao Zheng, Xiumei Mo
Tracheal injuries are one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, core-shell nanofibrous scaffold was fabricated to encapsulate bovine serum albumin & rhTGF-β3 (recombinant human transforming growth factor-β3) into the core of the nanofibers for tracheal cartilage regeneration. Characterization of the core-shell nanofibrous scaffold was carried out by scanning electron microscope (SEM), transmission electron microscope (TEM), laser scanning confocal microscopy (LSCM), and tensile mechanical test...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27770886/characterization-of-human-adipose-tissue-derived-stem-cells-in-vitro-culture-and-in-vivo-differentiation-in-a-temperature-sensitive-chitosan-%C3%AE-glycerophosphate-collagen-hybrid-hydrogel
#19
Kedong Song, Liying Li, Xinyu Yan, Wen Zhang, Yu Zhang, Yiwei Wang, Tianqing Liu
In this study, the interaction of human adipose tissue-derived stem cells (ADSCs) with chitosan/β-glycerophosphate/collagen (C/GP/Co) hybrid hydrogel was test, followed by investigating the capability of engineered adipose tissue formation using this ADSCs seeded hydrogel. The ADSCs were harvested and mixed with a C/GP/Co hydrogel followed by a gelation at 37°C and an in vitro culture. The results showed that the ADSCs within C/GP/Co hydrogels achieved a 30% of expansion over 7days in culture medium and encapsulated cell in C/GP/Co hydrogel demonstrated a characteristic morphology with high viability over 5days...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27770834/preclinical-study-of-mouse-pluripotent-parthenogenetic-embryonic-stem-cell-derivatives-for-the-construction-of-tissue-engineered-skin-equivalent
#20
Yang Rao, Jihong Cui, Lu Yin, Wei Liu, Wenguang Liu, Mei Sun, Xingrong Yan, Ling Wang, Fulin Chen
BACKGROUND: Embryonic stem cell (ESC) derivatives hold great promise for the construction of tissue-engineered skin equivalents (TESE). However, harvesting of ESCs destroys viable embryos and may lead to political and ethical concerns over their application. In the current study, we directed mouse parthenogenetic embryonic stem cells (pESCs) to differentiate into fibroblasts, constructed TESE, and evaluated its function in vivo. METHODS: The stemness marker expression and the pluripotent differentiation ability of pESCs were tested...
October 22, 2016: Stem Cell Research & Therapy
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