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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/28722162/cyanidin-suppresses-autophagic-activity-regulating-chondrocyte-hypertrophic-differentiation
#1
Zhen Cao, Song Huang, Ce Dou, Qiang Xiang, Shiwu Dong
Cartilage is a kind of special connective tissue which does not contain neither blood vessels nor lymphatics and nerves. Therefore, the damage in cartilage is difficult to be repaired spontaneously. Constructing tissue engineered cartilage provides a new technique for cartilage repairing. Mesenchymal stem cells (MSCs) possess a unique capability of self-renew and can differentiate into pre-chondrocytes which are frequently applied as seed cells in tissue engineering. However, in regenerated cartilage the chondrocytes derived from MSCs can hardly maintain homeostasis and preferentially present hypertrophic like phenotype...
July 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28715029/anisotropic-microfibrous-scaffolds-enhance-the-organization-and-function-of-cardiomyocytes-derived-from-induced-pluripotent-stem-cells
#2
Maureen Wanjare, Luqia Hou, Karina H Nakayama, Joseph J Kim, Nicholas P Mezak, Oscar J Abilez, Evangeline Tzatzalos, Joseph C Wu, Ngan F Huang
Engineering of myocardial tissue constructs is a promising approach for treatment of coronary heart disease. To engineer myocardial tissues that better mimic the highly ordered physiological arrangement and function of native cardiomyocytes, we generated electrospun microfibrous polycaprolactone scaffolds with either randomly oriented (14 μm fiber diameter) or parallel-aligned (7 μm fiber diameter) microfiber arrangement and co-seeded the scaffolds with human induced pluripotent stem cell-derived cardiomyocytes (iCMs) and endothelial cells (iECs) for up to 12 days after iCM seeding...
July 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28714856/use-of-human-aortic-extracellular-matrix-as-a-scaffold-for-construction-of-patient-specific-tissue-engineered-vascular-patch
#3
Liping Gao, Mingjun Du, Jingjing Lv, Sebastian Schmull, Ritai Huang, Jun Li
Abstract Synthetic or biologic materials are usually used to repair vascular malformation in congenital heart defects; however, non-autologous materials show both mismatch compliance and antigenicity, as well as a lack of recellularization on its surface. Here, we constructed a tissue-engineered vascular patch (TEVP) using decellularized extracellular matrix (ECM) scaffold obtained from excised human aorta during surgery, which was seeded with patient-derived bone marrow CD34-positive (CD34+) progenitor cells...
July 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#4
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28686585/constructing-cellular-niche-properties-by-localized-presentation-of-wnt-proteins-on-synthetic-surfaces
#5
Molly Lowndes, Sergi Junyent, Shukry J Habib
Wnt signaling is crucial during embryonic development and for the maintenance of adult tissues. Depending on the tissue type, the Wnt pathway can promote stem cell self-renewal and/or direct lineage commitment. Wnt proteins are subject to lipid modification, often restricting them to act in a localized manner on responsive cells. Most methods for inducing Wnt signaling in stem cell cultures do not control the spatial presentation of the protein. To recreate the local presentation of Wnt proteins often seen in vivo, we previously developed a method to immobilize the protein onto synthetic surfaces...
July 2017: Nature Protocols
https://www.readbyqxmd.com/read/28675007/-effect-of-hypoxia-on-the-biological-characteristics-of-human-dental-follicle-cells
#6
Liang Xi, Chen Guoqing, Tian Weidong
OBJECTIVE: This study aimed to investigate the effects of hypoxia on the characteristics of human dental follicle cells (hDFCs). METHODS: The tissue explant collagenase method was used to isolate hDFCs from young permanent teeth. The immunofluorescence technique was used to detect cell surface markers, and the multi-differentiation potential was detected by multilineage differentiation induction assay. Then, the hypoxic microenvironment was physically mimicked, and the cells were divided into the normoxia group (20%O₂) and the hypoxia group (2%O₂)...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28672891/osteogenic-potency-of-dedifferentiated-fat-cells-isolated-from-elderly-people-with-osteoporosis
#7
Wei Hao, Chuanqiang Jiang, Ming Jiang, Tian Wang, Xin Wang
Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis-specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28653139/spreading-proliferation-and-differentiation-of-human-dental-pulp-stem-cells-on-chitosan-scaffolds-immobilized-with-rgd-or-fibronectin
#8
Farzin Asghari Sana, Merve Çapkın Yurtsever, Gökçe Kaynak Bayrak, Ekin Özge Tunçay, Arlin S Kiremitçi, Menemşe Gümüşderelioğlu
Nowadays, human dental pulp stem cells (hDPSCs) became more attractive for therapeutic purposes because of their high proliferation and differentiation potential. Thus, coupling the desired cellular characteristics of hDPSCs with good biomaterial properties of the chitosan scaffolds provide an interesting approach for tissue engineering applications. On the other hand, scaffold surface modification is also needed to promote stem cell adhesion since chitosan lacks adhesion motifs to support direct cell anchorage...
June 26, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28641554/future-of-spermatogonial-stem-cell-culture-application-of-nanofiber-scaffolds
#9
Azar Shams, Neda Eslahi, Mansoureh Movahedin, Fariborz Izadyar, Hamidreza Asgari, Morteza Koruji
The spermatogonial stem cells (SSCs) are unique in mammals because they can transmit genetic information from generation to generation and it is of significant importance. In testes, Sertoli cells, peritubular myoid cells, Leydig cells and other interstitial cells contribute to the spermatogonial stem cell "niche". So, creation of niche in in vitro condition that mimics in vivo environment is essential to maintain functional characteristic of SSCs. Nanofiber matrices mimic the architecture and size scale of the natural extracellular matrix (ECM)...
June 22, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28629100/biomaterials-and-cells-for-cardiac-tissue-engineering-current-choices
#10
REVIEW
Rusha Chaudhuri, Madhumitha Ramachandran, Pearl Moharil, Megha Harumalani, Amit K Jaiswal
The major purpose of cardiac tissue engineering is to engineer cells on scaffolds and use it as a substitute to infarcted cardiac cells. With an ever-increasing risk of cardiac diseases there is an increasing need to have a stable and sustainable approach to cure such ailments. This review provides a comprehensive update on the cell sources and biomaterials essential for cardiac tissue engineering, ensuring their biocompatibility under a variety of conditions. Cells can be obtained from allogenic or autologous sources...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629021/novel-hipsc-based-tri-culture-for-pre-vascularization-of-calcium-phosphate-scaffold-to-enhance-bone-and-vessel-formation
#11
Chi Zhang, Kevin Hu, Xian Liu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
Vascularization of tissue-engineered bone is a critical step in maintaining cell viability and advancing cell performance in vivo. In this study, a novel tri-culture system was developed to elicit pre-vascularization of calcium phosphate cement (CPC) scaffold in which human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSMSCs) were seeded together with human umbilical vein endothelial cells (HUVECs) and pericytes. In a two-step methodology design, we first performed osteoinduction of the seeded hiPSMSCs on the CPC scaffold and then incorporated HUVECs and pericytes to the hiPSMSC-colonized CPC scaffold under a favorable culturing condition, with an objective to form a stable and functional capillary-like vascular network that sustained the engineered osseous tissue...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627740/biofabrication-of-soft-tissue-templates-for-engineering-the-bone-ligament-interface
#12
Ella Harris, Yurong Liu, Grainne Cuniffe, David Morrissey, Simon Carroll, Kevin Mulhall, Daniel J Kelly
Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition and function of the native enthesis. In the ligament-to-bone interface this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradation in tissue types facilitates the transfer of load between soft and hard structures while minimizing stress concentrations at the interface. Previous attempts to engineer the ligament-bone interface have utilized various scaffold materials with an array of various cell types and/or biological cues...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#13
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28608414/use-of-lecithin-to-control-fibre-morphology-in-electrospun-poly-%C3%AE%C2%B5-caprolactone-scaffolds-for-improved-tissue-engineering-applications
#14
Benjamin D M Coverdale, Julie E Gough, William W Sampson, Judith A Hoyland
We elucidate the effects of incorporating surfactants into electrospun poly (ε-caprolactone) (PCL) scaffolds on network homogeneity, cellular adherence and osteogenic differentiation. Lecithin was added with a range of concentrations to PCL solutions, which were electrospun to yield functionalised scaffolds. Addition of lecithin yielded a dose-dependent reduction in scaffold hydrophobicity, whilst reducing fibre width and hence increasing specific surface area. These changes in scaffold morphology were associated with increased cellular attachment of Saos-2 osteoblasts 3-hours post-seeding...
June 12, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28605808/rat-limbal-niche-cells-prevent-epithelial-stem-progenitor-cells-from-differentiation-and-proliferation-by-inhibiting-notch-signaling-pathway-in-vitro
#15
Jing Li, Szu-Yu Chen, Xin-Yue Zhao, Ming-Chang Zhang, Hua-Tao Xie
Purpose: Limbal niche cells (LNCs) play a pivotal role in regulating limbal epithelial stem/progenitor cells (LESCs). This study aimed to investigate whether Notch signaling is involved in LNCs' regulation of LESCs. Methods: Rat limbus was digested by dispase and collagenase, respectively. Limbal niche cells were isolated by serial passage of collagenase-digested cells on coated Matrigel in a modified embryonic stem cell medium (MESCM). Dispase-isolated cells, with or without LNCs, were seeded on three-dimensional (3D) Matrigel...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28586547/pcl-ha-microscaffolds-for-in-vitro-modular-bone-tissue-engineering
#16
Alessandra Totaro, Aurelio Salerno, Giorgia Imparato, Concepción Domingo, Francesco Urciuolo, Paolo Antonio Netti
The evolution of microscaffolds and bone-bioactive surfaces is a pivotal point in modular bone tissue engineering. In this study, the design and fabrication of porous polycaprolactone (PCL) microscaffolds functionalized with hydroxyapatite (HA) nanoparticles by means of a bio-safe and versatile thermally-induced phase separation process is reported. The ability of the as-prepared nanocomposite microscaffolds to support the adhesion, growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in standard and osteogenic media and using dynamic seeding/culture conditions was investigated...
June 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28576024/layer-by-layer-assembled-polyelectrolytes-on-honeycomb-like-porous-poly-%C3%AE%C2%B5-caprolactone-films-modulate-the-spatial-distribution-of-mesenchymal-stem-cells
#17
Yanli Liu, Jiazhu Xu, Yan Zhou, Zhaoyang Ye, Wen-Song Tan
Cellular behaviors can be affected by both surface chemistry and topography of biomaterials substrates. The object of the present study was to investigate how pore structure and bioactive molecules regulate the adhesion and proliferation of rabbit bone marrow-derived mesenchymal stem cells (rMSCs) in synergy. Poly(ε-caprolactone) (PCL) films with a honeycomb-like porous structure were fabricated via a breath-figure method, and then further coated with bioactive molecules including four combinations of polyelectrolytes (GEL/CS, GEL/HA, CHI/CS and CHI/HA) via a layer-by-layer self-assembly (LBL) process...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28575957/neural-differentiation-of-human-induced-pluripotent-stem-cells-on-polycaprolactone-gelatin-bi-electrospun-nanofibers
#18
Ali KarbalaeiMahdi, Mohsen Shahrousvand, Hamid Reza Javadi, Marzieh Ghollasi, Faezeh Norouz, Mehdi Kamali, Ali Salimi
In the present study, for the first time, polycaprolactone (PCL) and gelatin (GEL) were used for neural differentiation of human induced pluripotent stem cells (hiPSCs) in the form of bi-electrospun nanofibers. The electrospun fibers were evaluated by FTIR and tensile analysis. MTT assay was used to evaluate the toxicity on the scaffolds. The hiPSCs were seeded on the fibers and after 14days in neural differentiation medium. To confirm the differentiation, real-time PCR and immunocytochemistry (ICC) analyses were performed...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28573977/bioactive-glass-ceramic-with-two-crystalline-phases-bios-2p-for-bone-tissue-engineering
#19
Emanuela Ferraz, Gileade Freitas, Murilo Crovace, Oscar Peitl, Edgar Zanotto, Paulo Tambasco de Oliveira, Marcio Mateus Beloti, Adalberto Rosa
We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded with MSCs...
June 2, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28567273/bone-and-cartilage-differentiation-of-a-single-stem-cell-population-driven-by-material-interface
#20
Hannah Donnelly, Carol-Anne Smith, Paula E Sweeten, Nikolaj Gadegaard, Rm Dominic Meek, Matteo D'Este, Alvaro Mata, David Eglin, Matthew J Dalby
Adult stem cells, such as mesenchymal stem cells, are a multipotent cell source able to differentiate towards multiple cell types. While used widely in tissue engineering and biomaterials research, they present inherent donor variability and functionalities. In addition, their potential to form multiple tissues is rarely exploited. Here, we combine an osteogenic nanotopography and a chondrogenic hyaluronan hydrogel with the hypothesis that we can make a complex tissue from a single multipotent cell source with the exemplar of creating a three-dimensional bone-cartilage boundary environment...
January 2017: Journal of Tissue Engineering
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