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Stem cell AND seed cells AND tissue engineering

Chunxiang Feng, Jinqian Hu, Chang Liu, Shiliang Liu, Guiying Liao, Linjie Song, Xiaoyong Zeng
The increased incidence of stress urinary incontinence (SUI) in postmenopausal women has been proposed to be associated with a reduction in the level of 17-β estradiol (E2). E2 has also been shown to enhance the multi-differentiation ability of adipose-derived stem cells (ASCs) in vitro. However, studies on the potential value of E2 for tissue engineering in SUI treatment are rare. In the present study, we successfully fabricated myogenically differentiated ASCs (MD-ASCs), which were seeded onto a Poly(l-lactide)/Poly(e-caprolactone) electrospinning nano-scaffold, and incorporated E2 into the system, with the aim of improving the proliferation and myogenic differentiation of ASCs...
2016: PloS One
I Stefani, M A Asnaghi, J J Cooper-White, S Mantero
Cardiovascular diseases represent a major global health burden, with high rates of mortality and morbidity. Autologous grafts are commonly used to replace damaged or failing blood vessels, however such approaches are hampered by the scarcity of suitable graft tissue, donor site morbidity and poor long-term stability. Tissue engineering has been investigated as a means by which exogenous vessel grafts can be produced, with varying levels of success to date, a result of mismatched mechanical properties of these vessel substitutes and inadequate ex vivo vessel tissue genesis...
October 24, 2016: Journal of Tissue Engineering and Regenerative Medicine
Yibo Gan, Sukai Li, Pei Li, Yuan Xu, Liyuan Wang, Chen Zhao, Bin Ouyang, Bing Tu, Chengmin Zhang, Lei Luo, Xiangdong Luo, Xiumei Mo, Qiang Zhou
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP. The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation...
2016: Stem Cells International
Jingyu Yang, Xuening Chen, Tun Yuan, Xiao Yang, Yujiang Fan, Xingdong Zhang
In the latest decade, mesenchymal stem cells (MSCs) have wildly considered as a source of seeded cells in tissue engineering, not only because of its multi-differentiation potentials, but also due to its immunoregulation ability. The main immunoregulatory features of MSCs could be divided into low self-immunogenicity and secretion of soluble factors. In this study, we explored how scaffold structures modulated the secretion of soluble immunoregulatory factors in MSCs under an allogeneic cartilage tissue engineering background...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Jue Hu, Dan Kai, Hongye Ye, Lingling Tian, Xin Ding, Seeram Ramakrishna, Xian Jun Loh
Nerve tissue engineering (TE) requires biomimetic scaffolds providing essential chemical and topographical cues for nerve regeneration. Poly(glycerol sebacate) (PGS) is a biodegradable and elastic polymer that has gained great interest as a TE scaffolding biomaterial. However, uncured PGS is difficult to be electrospun into nanofibers. PGS would, therefore, require the addition of electrospinning agents. In this study, we modified PGS by using atom transfer radical polymerization (ATRP) to synthesize PGS-based copolymers with methyl methacrylate (MMA)...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Jing Wang, Binbin Sun, Lingling Tian, Xiaomin He, Qiang Gao, Tong Wu, Seeram Ramakrishna, Jinghao Zheng, Xiumei Mo
Tracheal injuries are one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, core-shell nanofibrous scaffold was fabricated to encapsulate bovine serum albumin & rhTGF-β3 (recombinant human transforming growth factor-β3) into the core of the nanofibers for tracheal cartilage regeneration. Characterization of the core-shell nanofibrous scaffold was carried out by scanning electron microscope (SEM), transmission electron microscope (TEM), laser scanning confocal microscopy (LSCM), and tensile mechanical test...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Kedong Song, Liying Li, Xinyu Yan, Wen Zhang, Yu Zhang, Yiwei Wang, Tianqing Liu
In this study, the interaction of human adipose tissue-derived stem cells (ADSCs) with chitosan/β-glycerophosphate/collagen (C/GP/Co) hybrid hydrogel was test, followed by investigating the capability of engineered adipose tissue formation using this ADSCs seeded hydrogel. The ADSCs were harvested and mixed with a C/GP/Co hydrogel followed by a gelation at 37°C and an in vitro culture. The results showed that the ADSCs within C/GP/Co hydrogels achieved a 30% of expansion over 7days in culture medium and encapsulated cell in C/GP/Co hydrogel demonstrated a characteristic morphology with high viability over 5days...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Yang Rao, Jihong Cui, Lu Yin, Wei Liu, Wenguang Liu, Mei Sun, Xingrong Yan, Ling Wang, Fulin Chen
BACKGROUND: Embryonic stem cell (ESC) derivatives hold great promise for the construction of tissue-engineered skin equivalents (TESE). However, harvesting of ESCs destroys viable embryos and may lead to political and ethical concerns over their application. In the current study, we directed mouse parthenogenetic embryonic stem cells (pESCs) to differentiate into fibroblasts, constructed TESE, and evaluated its function in vivo. METHODS: The stemness marker expression and the pluripotent differentiation ability of pESCs were tested...
October 22, 2016: Stem Cell Research & Therapy
Ines Westphal, Claudia Jedelhauser, Gregor Liebsch, Arnd Wilhelmi, Attila Aszodi, Matthias Schieker
Bone tissue engineering (BTE) utilizing biomaterial scaffolds and human mesenchymal stem cells (hMSCs) is a promising approach for the treatment of bone defects. The quality of engineered tissue is crucially affected by numerous parameters including cell density and the oxygen supply. In this study, a novel oxygen-imaging sensor was introduced to monitor the oxygen distribution in three dimensional (3D) scaffolds in order to analyze a new cell-seeding strategy. Immortalized hMSCs, pre-cultured in a monolayer for 30-40% or 70-80% confluence, were used to seed demineralized bone matrix (DBM) scaffolds...
October 17, 2016: Biotechnology and Bioengineering
A A Shumilova, M P Myltygashev, A K Kirichenko, E D Nikolaeva, T G Volova, E I Shishatskaya
The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid P(3HB) for regeneration of bone tissue defects. The ability of the P(3HB) implants to favor attachment and facilitate proliferation and directed differentiation of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. Tissue-engineered hybrid systems (grafts) constructed by using P(3HB) and P(3HB) in combination with osteoblasts were used in experiments on laboratory animals (n=48) with bone defect model...
October 14, 2016: Journal of Biomedical Materials Research. Part A
Tsai-Jung Lu, Fang-Yao Chiu, Hsiao-Ying Chiu, Ming-Chau Chang, Shih-Chieh Hung
Articular cartilage has a very limited capacity for self-repair, and mesenchymal stem cells (MSCs) have the potential to treat cartilage defects and osteoarthritis. However, in-depth mechanistic studies regarding their applications are required. Here, we demonstrated the use of chitosan film culture for promoting chondrogenic differentiation of MSCs. We found that MSCs formed spheres two days after seeding on dishes coated with chitosan. When MSCs were induced in a chondrogenic induction medium on chitosan films, the size of spheres continuously increased for up to 21 days...
October 12, 2016: Cell Transplantation
Luiz Alexandre Chisini, Marcus Cristian Muniz Conde, Jose Carlos Bernedo Alcázar, Adriana Fernandes da Silva, Jacques Eduardo Nör, Sandra Beatriz Chaves Tarquinio, Flávio Fernando Demarco
The aim of the present study was to evaluate the expression of transforming growth factor-β1 (TGF-β1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed. Empty pulp spaces of the tooth slice were filled with sodium chloride particles (250-425 µm)...
October 10, 2016: Brazilian Oral Research
Siddharth Shanbhag, Kamal Mustafa, Nikolaos Pandis, Jens R Nyengaard, Andreas Stavropoulos
The regenerative potential of tissue engineered bone constructs may be enhanced by in vitro co-culture and in vivo co-transplantation of vasculogenic and osteogenic (progenitor) cells. The objective of this study was to systematically review the literature to answer the focused question: in animal models, does co-transplantation of osteogenic and vasculogenic cells enhance bone regeneration in craniofacial defects, compared to solely osteogenic cell-seeded constructs? Following PRISMA guidelines, electronic databases were searched for controlled animal studies reporting co-transplantation of endothelial cells (EC) with mesenchymal stem cells (MSC) or osteoblasts (OB) in craniofacial critical-size bone defect (CSD) models...
October 12, 2016: Tissue Engineering. Part B, Reviews
Nolan B Skop, Frances Calderon, Cheul H Cho, Chirag D Gandhi, Steven W Levison
Tissue engineering using stem cells is widely used to repair damaged tissues in diverse biological systems; however, this approach has met with less success in regenerating the central nervous system (CNS). In this study we optimized and characterized the surface chemistry of chitosan-based scaffolds for CNS repair. To maintain radial glial cell (RGC) character of primitive neural precursors, fibronectin was adsorbed to chitosan. The chitosan was further modified by covalently linking heparin using genipin, which then served as a linker to immobilize fibroblast growth factor-2 (FGF-2), creating a multifunctional film...
October 2016: Journal of Tissue Engineering and Regenerative Medicine
Zhankui Zhao, Honglian Yu, Chengjuan Fan, Qingsheng Kong, Deqian Liu, Lin Meng
Ureter reconstruction is still a tough task for urologist. Cell-based tissue engineering serves a better technique for patients with long segments of ureter defect who need ureter reconstruction. In this study, we sought to evaluate the differentiation potential of adipose derived stem cells (ADSCs) into urothelial lineage and smooth muscle lineage and to assess the possibility of ureter reconstruction using differentiated cells seeded vessel extracellular matrix (VECM) in a rabbit model. ADSCs were isolated from adipose tissue and identified in vitro...
2016: American Journal of Translational Research
Andrew C Daly, Susan E Critchley, Emily M Rencsok, Daniel J Kelly
Cartilage is a dense connective tissue with limited self-repair capabilities. Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments. This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres. The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro...
October 7, 2016: Biofabrication
Henrique Almeida, Binulal Nelson Sathy, Ivan Dudurych, Conor Timothy Buckley, Fergal J O'Brien, Daniel John Kelly
Regenerating articular cartilage and fibrocartilaginous tissue such as the meniscus is still a challenge in orthopedic medicine. While a range of different scaffolds have been developed for joint repair, none have facilitated the development of a tissue that mimics the complexity of soft tissues such as articular cartilage. Furthermore, many of these scaffolds are not designed to function in mechanically challenging joint environments. The overall goal of this study was to develop a porous, biomimetic, shape-memory alginate scaffold for directing cartilage regeneration...
October 6, 2016: Tissue Engineering. Part A
Adam O'Reilly, Daniel John Kelly
Developing successful tissue engineering strategies requires an understanding of how cells within an implanted scaffold interacts with the host environment. The objective of this study was to use a computational mechanobiological model to explore how the design of a cell laden scaffold influences the spatial formation of cartilage and bone within an osteochondral defect. Tissue differentiation was predicted using a previously developed model in which cell fate depends on the local oxygen tension and the mechanical environment within a damaged joint...
October 6, 2016: Tissue Engineering. Part A
Yu Shrike Zhang, Andrea Arneri, Simone Bersini, Su-Ryon Shin, Kai Zhu, Zahra Goli-Malekabadi, Julio Aleman, Cristina Colosi, Fabio Busignani, Valeria Dell'Erba, Colin Bishop, Thomas Shupe, Danilo Demarchi, Matteo Moretti, Marco Rasponi, Mehmet Remzi Dokmeci, Anthony Atala, Ali Khademhosseini
Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium...
December 2016: Biomaterials
Han Xie, Melissa Chua, Intekhab Islam, Ricardo Bentini, Tong Cao, José Carlos Viana-Gomes, Antônio Hélio Castro Neto, Vinicius Rosa
OBJECTIVE: The objective was to investigate the potential of graphene (Gp) to induce odontogenic and osteogenic differentiation in dental pulp stem cells (DPSC). METHODS: Gp was produced by chemical vapor deposition. DPSC were seeded on Gp or glass (Gl). Cells were maintained in culture medium for 28 days. Every two days, culture medium from Gp was used to treat cells on Gl and vice versa. Mineralization and differentiation of DPSC on all substrates were evaluated after 14 and 28 days by alizarin red S staining, qPCR, immunofluorescence and FACS...
September 27, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
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