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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/28932678/precise-protein-photolithography-p-3-high-performance-biopatterning-using-silk-fibroin-light-chain-as-the-resist
#1
Wanpeng Liu, Zhitao Zhou, Shaoqing Zhang, Zhifeng Shi, Justin Tabarini, Woonsoo Lee, Yeshun Zhang, S N Gilbert Corder, Xinxin Li, Fei Dong, Liang Cheng, Mengkun Liu, David L Kaplan, Fiorenzo G Omenetto, Guozheng Zhang, Ying Mao, Tiger H Tao
Precise patterning of biomaterials has widespread applications, including drug release, degradable implants, tissue engineering, and regenerative medicine. Patterning of protein-based microstructures using UV-photolithography has been demonstrated using protein as the resist material. The Achilles heel of existing protein-based biophotoresists is the inevitable wide molecular weight distribution during the protein extraction/regeneration process, hindering their practical uses in the semiconductor industry where reliability and repeatability are paramount...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28926122/stem-cells-and-differentiated-cells-differ-in-their-sensitivity-to-urine-in-vitro
#2
Marta Pokrywczyńska, Tomasz Kloskowski, Daria Balcerczyk, Monika Buhl, Arkadiusz Jundziłł, Maciej Nowacki, Kaja Męcińska-Jundziłł, Tomasz Drewa
INTRODUCTION: Urinary tract regeneration using tissue engineering is one of the most challenging issues in the field of reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after the implantation. The outcome of urinary bladder regeneration is depended on the ability of these cells to survive, proliferate and regenerate. The aim of this study was to compare a sensitivity of three different cell lines to urine in vitro. MATERIAL AND METHODS: Three different cell lines were isolated from porcine bladder (urothelial cells, UCs and smooth muscle cells, SMCs) and adipose tissue (adipose-derived stem cells, ADSCs)...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28921540/imaging-stem-cell-distribution-growth-migration-and-differentiation-in-3-d-scaffolds-for-bone-tissue-engineering-using-mesoscopic-fluorescence-tomography
#3
Qinggong Tang, Charlotte Piard, Jonathan Lin, Kai Nan, Ting Guo, John Caccamese, John Fisher, Yu Chen
Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2∼3 mm)...
September 16, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28917149/a-comparison-study-between-electrospun-polycaprolactone-and-piezoelectric-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-scaffolds-for-bone-tissue-engineering
#4
Svetlana N Gorodzha, Albert R Muslimov, Dina S Syromotina, Alexander S Timin, Nikolai Y Tcvetkov, Kirill V Lepik, Aleksandra V Petrova, Maria A Surmeneva, Dmitry A Gorin, Gleb B Sukhorukov, Roman A Surmenev
In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800nm to 12μm...
September 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28914404/development-and-characterization-of-hybrid-tubular-structure-of-plcl-porous-scaffold-with-hmscs-ecs-cell-sheet
#5
Azizah Intan Pangesty, Takaaki Arahira, Mitsugu Todo
Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28914400/an-in-vivo-study-on-endothelialized-vascular-grafts-produced-by-autologous-biotubes-and-adipose-stem-cells-adscs
#6
Yu Chieh Tseng, Jun Neng Roan, Ying Chiang Ho, Chih Chan Lin, Ming Long Yeh
Currently, commercial synthetic vascular grafts made from Dacron and ePTFE for small-diameter, vascular applications (<6 mm) show limited reendothelization and are less compliant, often resulting in thrombosis and intimal hyperplasia. Although good blood compatibility can be achieved in autologous arteries and veins, the number of high quality harvest sites is limited, and the grafts are size-mismatched for use in the fistula or cardiovascular bypass surgery; thus, alternative small graft substitutes must be developed...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28898756/bioreactor-culture-duration-of-engineered-constructs-influences-bone-formation-by-mesenchymal-stem-cells
#7
Debika Mitra, Jacklyn Whitehead, Osamu W Yasui, J Kent Leach
Perfusion culture of mesenchymal stem cells (MSCs) seeded in biomaterial scaffolds provides nutrients for cell survival, enhances extracellular matrix deposition, and increases osteogenic cell differentiation. However, there is no consensus on the appropriate perfusion duration of cellular constructs in vitro to boost their bone forming capacity in vivo. We investigated this phenomenon by culturing human MSCs in macroporous composite scaffolds in a direct perfusion bioreactor and compared their response to scaffolds in continuous dynamic culture conditions on an XYZ shaker...
September 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28893083/tissue-engineering-of-urethra-systematic-review-of-recent-literature
#8
Stanislav Žiaran, Martina Galambošová, L'uboš Danišovič
The purpose of this article was to perform a systematic review of the recent literature on urethral tissue engineering. A total of 31 articles describing the use of tissue engineering for urethra reconstruction were included. The obtained results were discussed in three groups: cells, scaffolds, and clinical results of urethral reconstructions using these components. Stem cells of different origin were used in many experimental studies, but only autologous urothelial cells, fibroblasts, and keratinocytes were applied in clinical trials...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28888046/gap-junctional-intercellular-communication-in-adipose-derived-stromal-stem-cells-is-cell-density-dependent-and-positively-impacts-adipogenic-differentiation
#9
Wiesner Miriam, Berberich Oliver, Hoefner Christiane, Blunk Torsten, Bauer-Kreisel Petra
Adipose-derived stromal/stem cells (ASCs) represent a widely used cell source with multi-lineage differentiation capacity in approaches for tissue engineering and regenerative medicine. Despite the multitude of literature on their differentiation capacity, little is reported about the physiological properties contributing to and controlling the process of lineage differentiation. Direct intercellular communication between adjacent cells via gap junctions has been shown to modulate differentiation processes in other cell types, with connexin 43 (Cx43) being the most abundant isoform of the gap junction-forming connexins...
September 9, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28888019/in-vivo-characterization-of-a-3d-hybrid-scaffold-based-on-pcl-decellularized-aorta-for-tracheal-tissue-engineering
#10
Fariba Ghorbani, Lida Moradi, Mohammad Behgam Shadmehr, Shahin Bonakdar, Atosa Droodinia, Farzaneh Safshekan
INTRODUCTION: As common treatments for long tracheal stenosis are associated with several limitations, tracheal tissue engineering is considered as an alternative treatment. AIM OF STUDY: This study aimed at preparing a hybrid scaffold, based on biologic and synthetic materials for tracheal tissue engineering. MATERIALS AND METHODS: Three electrospun polycaprolactone (PCL) scaffolds, namely E1 (pure PCL), E2 (collagen-coated PCL) and E3 (PCL blended with collagen) were prepared...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28884519/gelatin-methacrylate-scaffold-for-bone-tissue-engineering-the-influence-of-polymer-concentration
#11
Nehar Celikkin, Simone Mastrogiacomo, Jakub Jaroszewicz, X Frank Walboomers, Wojciech Swieszkowski
Gelatin Methacrylate (GelMA) is an inexpensive, photocrosslinkable, cell-responsive hydrogel which has drawn attention for a wide range of tissue engineering applications. The potential of GelMA scaffolds was demonstrated to be tunable for different TE applications through modifying the polymer concentration, methacrylation degree or UV light intensity. Despite the promising results of GelMA hydrogels in tissue engineering, the influence of polymer concentration for bone tissue engineering scaffolds was not established yet...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28880313/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffolds-modified-with-hydroxyapatite-and-poly-propylene-fumarate-and-their-effects-on-the-healing-of-rabbit-femur-defects
#12
Senem Buyuksungur, Tugba Endogan Tanir, Arda Buyuksungur, Ezgi Irem Bektas, Gamze Torun Kose, Deniz Yucel, Tahsin Beyzadeoglu, Engin Cetinkaya, Cagri Yenigun, Ergin Tönük, Vasif Hasirci, Nesrin Hasirci
A large variety of approaches have been used to treat large and irregular shaped bone defects with less than optimal success due to material or design issues. In recent years patient specific constructs prepared by additive manufacturing provided a solution to the need for shaping implants to fit irregular defects in the surgery theater. In this study, cylindrical disks of poly(ε-caprolactone) (PCL) were printed by fused deposition modeling and modified with nanohydroxyapatite (HAp) and poly(propylene fumarate) (PPF) to create a mechanically strong implant with well-defined pore size and porosity, controllable surface hydrophilicity (with PPF) and osteoconductivity (with HAp)...
September 7, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28872512/the-effects-of-adipose-derived-stem-cells-differentiated-into-endothelial-cells-and-osteoblasts-on-healing-of-critical-size-calvarial-defects
#13
Hakan Orbay, Brittany Busse, Jonathan Kent Leach, David E Sahar
Delayed vascularization and resultant resorption limits the clinical use of tissue engineered bony constructs. The objective of this study is to develop a strategy to accelerate the neovascularization of tissue-engineered bony constructs using endothelial differentiated adipose-derived stem cells (ASC). The authors harvested ASC from inguinal fat pads of male Lewis rats (n = 5) and induced toward endothelial and osteoblastic lineages. The authors created critical size calvarial defects on male Lewis rats (n = 30) and randomized the animals into 4 groups...
September 1, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28872083/-repair-of-articular-cartilage-defects-by-autologous-bone-mesenchymal-stem-cells-and-allogeneic-costal-chondrocytes-in-the-knee-of-wuzhishan-miniature-pigs
#14
Cheng Yang, Jiangdong Ni, Shou Zhang, Zhongcheng Fan
To investigate the feasibility of construction of tissue engineered cartilage by co-culture of bone marrow mesenchymal stem cells (BMSCs) and costal chondrocytes (CCs), and to provide theoretical basis and experimental basis for clinical repair of articular cartilage defects by Wuzhishan miniature pig knee cartilage defects with co-cultured cells.
 Methods: Density gradient centrifugation method was used to isolate BMSCs from Wuzhishan miniature pig. The double enzyme digestion method was used to isolate CCs...
August 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28869032/tissue-engineering-as-an-adjunct-to-pelvic-reconstructive-surgery
#15
Hanna Jangö
This PhD-thesis is based on animal studies and comprises three original papers and unpublished data. The studies were con-ducted during my employment as a research fellow at the Department of Obstetrics and Gynecology, Herlev University Hospital, Denmark. New strategies for surgical reconstruction of pelvic organ pro-lapse (POP) are warranted. Traditional native tissue repair may be associated with poor long-term outcome and augmentation with permanent polypropylene meshes is associated with frequent and severe adverse effects...
August 2017: Danish Medical Journal
https://www.readbyqxmd.com/read/28866191/nanosized-cap-silk-fibroin-pcl-peg-pcl-pcl-based-bilayer-membranes-for-guided-bone-regeneration
#16
Sibel Türkkan, A Engin Pazarçeviren, Dilek Keskin, Nesrin E Machin, Özgür Duygulu, Ayşen Tezcaner
Guided bone regeneration (GBR) concept has been developed to prevent the formation of non-functional scar tissue layer on defect site by undertaking barrier role. In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA)(1) and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28862380/fetal-bone-marrow-derived-mesenchymal-stem-stromal-cells-enhance-humanization-and-bone-formation-of-bmp7-loaded-scaffolds
#17
Abbas Shafiee, Jeremy G Baldwin, Jatin Patel, Boris M Holzapfel, Nicholas M Fisk, Kiarash Khosrotehrani, Dietmar W Hutmacher
Tissue engineered constructs built with human cells capable of generating a bone-like organ within the mouse have attracted considerable interest over the past decade. Here, we aimed to compare the utility of human mesenchymal stem/stromal cells (MSC) isolated from fetal term placenta (fPL-MSC) and fetal first trimester bone marrow (fBM-MSC) in a polycaprolactone scaffold/BMP7-based model in nude mice. Furthermore, fPL-MSC were co-seeded with fetal placenta-derived endothelial colony forming cells (ECFC) to assess the impact of ECFC on fPL-MSC osteogenesis...
September 1, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28855683/tracheal-reconstruction-with-a-free-vascularized-myofascial-flap-preclinical-investigation-in-a-porcine-model-to-human-clinical-application
#18
Won Shik Kim, Jae Won Chang, Woo Soon Jang, Young Joon Seo, Mi-Lan Kang, Hak-Joon Sung, Da Hee Kim, Jung Min Kim, Jae Hong Park, Myung Jin Ban, Gina Na, Seung Ho Shin, Hyung Kwon Byeon, Yoon Woo Koh, Se-Heon Kim, Hong Koo Baik, Eun Chang Choi
Although there are various methods for tracheal reconstruction, such as a simple approximation with suturing and coverage with adjacent soft tissue or muscle, large defects >50% of the tracheal length still present a clinical challenge. Tissue engineering, a recent promising way to possibly resolve this problem, requires a long preparatory period for stem cell seeding on a scaffold and relatively invasive procedures for stem cell harvesting. As an alternative, we used a vascularized myofascial flap for tracheal reconstruction...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28845088/platelet-rich-plasma-as-an-autologous-and-proangiogenic-cell-delivery-system
#19
Jessica Zahn, Markus Loibl, Christoph Sprecher, Michael Nerlich, Mauro Alini, Sophie Verrier, Marietta Herrmann
Angiogenesis is a key factor in early stages of wound healing and is crucial for the repair of vascularized tissues such as the bone. However, supporting timely revascularization of the defect site still presents a clinical challenge. Tissue engineering approaches delivering endothelial cells or prevascularized constructs may overcome this problem. In the current study, we investigated platelet-rich plasma (PRP) gels as autologous, injectable cell delivery systems for prevascularized constructs. PRP was produced from human thrombocyte concentrates...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28844179/noninvasive-absolute-epr-oxygen-imaging-for-the-assessment-of-tissue-graft-oxygenation
#20
Mrignayani Kotecha, Boris Epel, Sriram Ravindran, Deborah Dorcemus, Syam P Nukavarapu, Howard J Halpern
Oxygen is the single most important molecule for sustaining life and therefore an important variable in tissue engineering and regenerative medicine (TERM). It has been shown that the change in oxygen concentration in an artificial or engineered tissue graft affects cell survival, differentiation, and tissue growth in profound ways. However, at present, there are no reliable methods to map partial oxygen pressure in growing artificial tissues. Here, we adapt and test the suitability of electron paramagnetic resonance oxygen imaging (EPROI) in assessing tissue graft oxygenation in vitro...
August 26, 2017: Tissue Engineering. Part C, Methods
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