keyword
MENU ▼
Read by QxMD icon Read
search

Stem cell AND seed cells AND tissue engineering

keyword
https://www.readbyqxmd.com/read/28325151/adipose-tissue-derived-pericytes-for-cartilage-tissue-engineering
#1
Jin Xin Zhang, Chun Yan Du, Wei Min Guo, Pan Li, Shu Yun Liu, Zhi Guo Yuan, Jian Hua Yang, Xun Sun, He Yong Yin, Quan Yi Guo, Chen Fu Zhou
Mesenchymal stem cells (MSCs) represent a promising alternative source for cartilage tissue engineering. However, MSC culture is labor-intensive, so these cells cannot be applied immediately to regenerate cartilage for clinical purposes. Risks during the ex vivo expansion of MSCs, such as infection and immunogenicity, can be a bottleneck in their use in clinical tissue engineering. As a novel stem cell source, pericytes are generally considered to be the origin of MSCs. Pericytes do not have to undergo time-consuming ex vivo expansion because they are uncultured cells...
March 21, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28320452/compared-to-the-amniotic-membrane-wharton-s-jelly-may-be-a-more-suitable-source-of-mesenchymal-stem-cells-for-cardiovascular-tissue-engineering-and-clinical-regeneration
#2
Lei Pu, Mingyao Meng, Jian Wu, Jing Zhang, Zongliu Hou, Hui Gao, Hui Xu, Boyu Liu, Weiwei Tang, Lihong Jiang, Yaxiong Li
BACKGROUND: The success of developing cardiovascular tissue engineering (CTE) grafts greatly needs a readily available cell substitute for endothelial and interstitial cells. Perinatal annexes have been proposed as a valuable source of mesenchymal stem cells (MSCs) for tissue engineering and regenerative medicine. The objective of the present study is to evaluate the potential of human Wharton's jelly MSCs (WJ-MSCs) and amniotic membrane MSCs (AM-MSCs) as a seeding cell in CTE and cardiovascular regenerative medicine...
March 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28316275/central-growth-factor-loaded-depots-in-bone-tissue-engineering-scaffolds-for-enhanced-cell-attraction
#3
Mandy Quade, Sven Knaack, Ashwini Rahul Akkineni, Anastasia Gabrielyan, Anja Lode, Angela Roesen-Wolff, Michael Gelinsky
Tissue engineering, i.e. the application of stem and progenitor cells in combination with an engineered extracellular matrix, is a promising strategy for bone regeneration. However, its success is limited by the lack of vascularization after implantation. The concept of in situ tissue engineering envisages the recruitment of cells necessary for tissue regeneration from the host environment foregoing ex vivo cell seeding of the scaffold. In this study, we developed a novel scaffold system for enhanced cell attraction which is based on biomimetic mineralized collagen scaffolds equipped with a central biopolymer depot loaded with chemotactic agents...
March 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28298644/the-combination-of-three-dimensional-and-rotary-cell-culture-system-promotes-the-proliferation-and-maintains-the-differentiation-potential-of-rat-bmscs
#4
Yilong Tang, Yan Xu, Zhifeng Xiao, Yannan Zhao, Jing Li, Sufang Han, Lei Chen, Bin Dai, Ling Wang, Bing Chen, Hong Wang
Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs' proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287922/co-seeding-human-endothelial-cells-with-human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-on-calcium-phosphate-scaffold-enhances-osteogenesis-and-vascularization-in-rats
#5
Xian Liu, Wenchuan Chen, Chi Zhang, Wahwah Thein-Han, Kevin Hu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
A major challenge in repairing large bone defects with tissue-engineered constructs is the poor vascularization in the defect. The lack of vascular networks leads to insufficient oxygen and nutrients supply, which compromises the survival of seeded cells. To achieve favorable regenerative effects, prevascularization of tissue-engineered constructs by co-culturing of endothelial cells and bone cells is a promising strategy. The aim of this study was to investigate the effects of human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) co-cultured with human umbilical vein endothelial cells (HUVECs) for prevascularization of calcium phosphate cement (CPC) scaffold on bone regeneration in vivo for the first time...
March 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28283336/tissue-engineering-with-peripheral-blood-derived-mesenchymal-stem-cells-promotes-the-regeneration-of-injured-peripheral-nerves
#6
Mengjie Pan, Xianghai Wang, Yijing Chen, Shangtao Cao, Jinkun Wen, Guofeng Wu, Yuanyuan Li, Lixia Li, Changhui Qian, Zhenqi Qin, Zhenlin Li, Dandan Tan, Zhihao Fan, Wutian Wu, Jiasong Guo
Peripheral nerve injury repair can be enhanced by Schwann cell (SC) transplantation, but clinical applications are limited by the lack of a cell source. Thus, alternative systems for generating SCs are desired. Herein, we found the peripheral blood-derived mesenchymal stem cells (PBMSCs) could be induced into SC like cells with expressing SC-specific markers (S100, P75NTR and CNPase) and functional factors (NGF, NT-3, c-Fos, and Krox20). When the induced PBMSCs (iPBMSCs) were transplanted into crushed rat sciatic nerves, they functioned as SCs by wrapping the injured axons and expressing myelin specific marker of MBP...
March 7, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28279224/urethral-reconstruction-with-autologous-urine-derived-stem-cells-seeded-in-three-dimensional-porous-small-intestinal-submucosa-in-a-rabbit-model
#7
Yang Liu, Wenjun Ma, Bo Liu, Yangcai Wang, Jiaqiang Chu, Geng Xiong, Lianju Shen, Chunlan Long, Tao Lin, Dawei He, Denis Butnaru, Lyundup Alexey, Yuanyuan Zhang, Deying Zhang, Guanghui Wei
BACKGROUND: Urethral reconstruction is one of the great surgical challenges for urologists. A cell-based tissue-engineered urethra may be an alternative for patients who have complicated long strictures and need urethral reconstruction. Here, we demonstrated the feasibility of using autologous urine-derived stem cells (USCs) seeded on small intestinal submucosa (SIS) to repair a urethral defect in a rabbit model. METHODS: Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested...
March 9, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28272494/an-innovative-approach-for-enhancing-bone-defect-healing-using-plga-scaffolds-seeded-with-extracorporeal-shock-wave-treated-bone-marrow-mesenchymal-stem-cells-bmscs
#8
Youbin Chen, Jiankun Xu, Zhonglian Huang, Menglei Yu, Yuantao Zhang, Hongjiang Chen, Zebin Ma, Haojie Liao, Jun Hu
Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28269745/histological-evaluations-of-apatite-fiber-scaffold-cultured-with-mesenchymal-stem%C3%A2-cells-by-implantation-at-rat-subcutaneous%C3%A2-tissue
#9
Kitaru Suzuki, Kohei Nagata, Tomohiro Yokota, Michiyo Honda, Mamoru Aizawa
BACKGROUND: There is a strong impetus for the development of alternative treatments for bone disease that avoid the complications associated with autografts and allografts. To address this, we previously developed porous apatite-fiber scaffolds (AFSs) which have three-dimensional interconnected pores, and constructed tissue-engineered bone by culturing rat bone marrow cells (RBMCs) using AFSs in a radial-flow bioreactor (RFB). OBJECTIVE: To generate additional baseline data for the development of tissue-engineered bone constructed for clinical application using a RFB, we cultured RBMCs using AFSs under static conditions (hereafter, RBMC AFS culture), and monitored RBMC growth and differentiation characteristics in vitro, and two weeks after subcutaneous inoculation into recipient rats...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28261617/repair-of-osteochondral-defects-using-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-in-a-rabbit-model
#10
Shuyun Liu, Yanhui Jia, Mei Yuan, Weimin Guo, Jingxiang Huang, Bin Zhao, Jiang Peng, Wenjing Xu, Shibi Lu, Quanyi Guo
Umbilical cord Wharton's jelly-derived mesenchymal stem cell (WJMSC) is a new-found mesenchymal stem cell in recent years with multiple lineage potential. Due to its abundant resources, no damage procurement, and lower immunogenicity than other adult MSCs, WJMSC promises to be a good xenogenous cell candidate for tissue engineering. This in vivo pilot study explored the use of human umbilical cord Wharton's jelly mesenchymal stem cells (hWJMSCs) containing a tissue engineering construct xenotransplant in rabbits to repair full-thickness cartilage defects in the femoral patellar groove...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28260890/preparation-of-a-nano-and-micro-fibrous-decellularized-scaffold-seeded-with-autologous-mesenchymal-stem-cells-for-inguinal-hernia-repair
#11
Yinlong Zhang, Yuanyuan Zhou, Xu Zhou, Bin Zhao, Jie Chai, Hongyi Liu, Yifei Zheng, Jinling Wang, Yaozong Wang, Yilin Zhao
Prosthetic meshes used for hernioplasty are usually complicated with chronic pain due to avascular fibrotic scar or mesh shrinkage. In this study, we developed a tissue-engineered mesh (TEM) by seeding autologous bone marrow-derived mesenchymal stem cells onto nanosized fibers decellularized aorta (DA). DA was achieved by decellularizing the aorta sample sequentially with physical, mechanical, biological enzymatic digestion, and chemical detergent processes. The tertiary structure of DA was constituted with micro-, submicro-, and nanosized fibers, and the original strength of fresh aorta was retained...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28256803/chitosan-agarose-scaffolds-supports-chondrogenesis-of-human-wharton-s-jelly-mesenchymal-stem-cells
#12
L P Merlin Rajesh Lal, G K Suraishkumar, Prabha D Nair
Tissue engineering strategies for cartilage aim to restore the complex biomechanical and biochemical properties of the native cartilage. To mimic the in-vivo microenvironment, we developed a novel scaffold based on chitosan-agarose (CHAG scaffold) resembling the properties of native cartilage extracellular matrix that aids in-vitro cartilage formation. The CHAG scaffolds had pore size ranging from 75 µm to 300 µm and the degradation of 18% over 6 months in PBS. L929 cells and Human Wharton's Jelly Mesenchymal Stem Cells (HWJ-MSCs) attached well and grew in the CHAG scaffolds...
March 3, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28253234/engineering-a-humanized-bone-organ-model-in-mice-to-study-bone-metastases
#13
Laure C Martine, Boris M Holzapfel, Jacqui A McGovern, Ferdinand Wagner, Verena M Quent, Parisa Hesami, Felix M Wunner, Cedryck Vaquette, Elena M De-Juan-Pardo, Toby D Brown, Bianca Nowlan, Dan Jing Wu, Cosmo Orlando Hutmacher, Davide Moi, Tatiana Oussenko, Elia Piccinini, Peter W Zandstra, Roberta Mazzieri, Jean-Pierre Lévesque, Paul D Dalton, Anna V Taubenberger, Dietmar W Hutmacher
Current in vivo models for investigating human primary bone tumors and cancer metastasis to the bone rely on the injection of human cancer cells into the mouse skeleton. This approach does not mimic species-specific mechanisms occurring in human diseases and may preclude successful clinical translation. We have developed a protocol to engineer humanized bone within immunodeficient hosts, which can be adapted to study the interactions between human cancer cells and a humanized bone microenvironment in vivo. A researcher trained in the principles of tissue engineering will be able to execute the protocol and yield study results within 4-6 months...
April 2017: Nature Protocols
https://www.readbyqxmd.com/read/28252220/metabolic-oscillations-in-co-cultures-of-hepatocytes-and-mesenchymal-stem-cells-effects-of-seeding-arrangement-and-culture-mixing
#14
Dalia Abdelrahim Alzebdeh, Howard William Matthew
In vitro assembly of functional liver tissue is a prerequisite for the transplantation of tissue-engineered livers. There is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of implantable liver systems is currently limited by the difficulty of assembling three dimensional hepatocyte cultures of a useful size, while maintaining full cell viability. Recent reports have demonstrated that bone marrow mesenchymal stem cells (BM-MSCs) can provide a number of cues promoting hepatocyte growth and development...
March 2, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28245809/mesenchymal-stem-cells-and-myoblast-differentiation-under-hgf-and-igf-1-stimulation-for-3d-skeletal-muscle-tissue-engineering
#15
R Witt, A Weigand, A M Boos, A Cai, D Dippold, A R Boccaccini, D W Schubert, M Hardt, C Lange, A Arkudas, R E Horch, J P Beier
BACKGROUND: Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding skeletal muscle cells on aligned constructs enhance the formation of myotubes as well as cell alignment and may provide a further step towards the clinical application of engineered skeletal muscle...
February 28, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28243954/knee-cartilage-regeneration-with-umbilical-cord-mesenchymal-stem-cells-embedded-in-collagen-scaffold-using-dry-arthroscopy-technique
#16
B Sadlik, G Jaroslawski, D Gladysz, M Puszkarz, M Markowska, K Pawelec, D Boruczkowski, T Oldak
Articular cartilage injuries lead to progressive degeneration of the joint with subsequent progression to osteoarthritis, which currently becomes a serious health and economic issue. Due to limited capability for self-regeneration, cartilage repair remains a challenge for the present-day orthopedics. Currently, available therapeutic methods fail to provide satisfactory results. A search for other strategies that could regenerate a hyaline-like tissue with a durable effect and adequate mechanical properties is underway...
February 28, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28238143/electrospun-nanofiber-scaffolds-and-their-hydrogel-composites-for-the-engineering-and-regeneration-of-soft-tissues
#17
Ohan S Manoukian, Rita Matta, Justin Letendre, Paige Collins, Augustus D Mazzocca, Sangamesh G Kumbar
Electrospinning has emerged as a simple, elegant, and scalable technique that can be used to fabricate polymeric nanofibers. Pure polymers as well as blends and composites of both natural and synthetic ones have been successfully electrospun into nanofiber matrices for many biomedical applications. Tissue-engineered medical implants, such as polymeric nanofiber scaffolds, are potential alternatives to autografts and allografts, which are short in supply and carry risks of disease transmission. These scaffolds have been used to engineer various soft tissues, including connective tissues, such as skin, ligament, and tendon, as well as nonconnective ones, such as vascular, muscle, and neural tissue...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28230598/analysis-of-the-adherence-of-dental-pulp-stem-cells-on-two-dimensional-and-three-dimensional-silk-fibroin-based-biomaterials-applications-in-regenerative-dentistry
#18
María Pilar Pecci-Lloret, Mar Vera-Sánchez, Salvador Aznar-Cervantes, David García-Bernal, Ricardo Oñate Sánchez, Miguel Ramón Pecci-Lloret, José María Moraleda, José Luis Cenis, Francisco Javier Rodríguez-Lozano
Among various biomaterials used as scaffolds in tissue engineering, silk fibroin is a highly attractive material. A scaffold should be biocompatible and nontoxic, with optimal physical features and mechanical properties. For this reason, tissue-engineering approaches in regenerative medicine have focused on investigating the biocompatibility of possible biomaterials by analyzing cell-scaffold interaction properties. The aim of the present study was to examine the biocompatibility of silk fibroin as a film (two-dimensional [2D]) and a scaffold (three-dimensional [3D]) after being cellularized with human dental pulp stem cells (hDPSCs)...
February 22, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28229424/three-dimensional-printed-scaffolds-with-multipotent-mesenchymal-stromal-cells-for-rabbit-mandibular-reconstruction-and-engineering
#19
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, Simon D Tran
Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28222169/decellularized-wharton-s-jelly-from-human-umbilical-cord-as-a-novel-3d-scaffolding-material-for-tissue-engineering-applications
#20
Sushma Jadalannagari, Gabriel Converse, Christopher McFall, Eric Buse, Michael Filla, Maria T Villar, Antonio Artigues, Adam J Mellot, Jinxi Wang, Michael S Detamore, Richard A Hopkins, Omar S Aljitawi
In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton's jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized Wharton's jelly matrix (DWJM) contained 0.66 ± 0.12 μg/mg sulfated glycosaminoglycans (GAGs), and was abundant in hyaluronic acid, and completely devoid of cells...
2017: PloS One
keyword
keyword
39241
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"