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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/29340876/biomaterials-and-regenerative-medicine-in-urology
#1
N F Davis, E M Cunnane, M R Quinlan, J J Mulvihill, N Lawrentschuk, D M Bolton, M T Walsh
Autologous gastrointestinal tissue is the gold standard biomaterial for urinary tract reconstruction despite its long-term neuromechanical and metabolic complications. Regenerative biomaterials have been proposed as alternatives; however many are limited by a poor host derived regenerative response and deficient supportive elements for effective tissue regeneration in vivo. Urological biomaterials are sub-classified into xenogenic extracellular matrices (ECMs) or synthetic polymers. ECMs are decellularised, biocompatible, biodegradable biomaterials derived from animal organs...
January 17, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29338385/does-the-mesenchymal-stem-cell-source-influence-smooth-muscle-regeneration-in-tissue-engineered-urinary-bladders
#2
Marta Pokrywczynska, Arkadiusz Jundzill, Karolina Warda, Lukasz Buchholz, Marta Rasmus, Jan Adamowicz, Magdalena Bodnar, Andrzej Marszalek, Anna Helmin-Basa, Jacek Michalkiewicz, Maciej Gagat, Alina Grzanka, Malgorzata Frontczak-Baniewicz, Agata Magdalena Gastecka, Tomasz Kloskowski, Maciej Nowacki, Camillo Ricordi, Tomasz Drewa
A variety of tissue engineering techniques utilizing different cells and biomaterials are currently being explored to construct urinary bladder walls de novo, but so far no approach is clearly superior. The aim of this study was to determine whether mesenchymal stem cells (MSCs) isolated from different sources, (bone marrow [BM-MSCs] and adipose tissue [ADSCs]), differ in their potential to regenerate smooth muscles in tissue-engineered urinary bladders and to determine an optimal number of MSCs for urinary bladder smooth muscle regeneration...
November 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29335199/3-d-mineralized-silk-fibroin-polycaprolactone-composite-scaffold-modified-with-polyglutamate-conjugated-with-bmp-2-peptide-for-bone-tissue-engineering
#3
Jingjing Luo, Haitao Zhang, Jiang Zhu, Xiaokang Cui, Junjiu Gao, Xin Wang, Jie Xiong
In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated with BMP-2 peptide onto silk fibroin (SF)/polycaprolactone (PCL) (SF/PCL) blend nanofibers. The morphology, composition, and mineralisation, were confirmed by FE-SEM, XRD, and FT-IR spectroscopy...
December 28, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29331090/enhanced-chondrogenesis-of-mesenchymal-stem-cells-over-silk-fibroin-chitosan-chondroitin-sulfate-three-dimensional-scaffold-in-dynamic-culture-condition
#4
Parinita Agrawal, Krishna Pramanik, Varshini Vishwanath, Amit Biswas, Akalabya Bissoyi, Pradeep Kumar Patra
Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37-212 µm, contact angle 46...
January 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29327677/autologous-smooth-muscle-progenitor-cells-enhance-regeneration-of-tissue-engineered-bladder
#5
Liuhua Zhou, Jiadong Xia, Pengji Wang, Ruipeng Jia, Jun Hua Zheng, Xudong Yao, Yun Chen, Yutian Dai, Bin Yang
Tissue engineering techniques provide a great potential to de novo construct a histological bladder. Smooth muscle regeneration is extremely important for the functional recovery of engineered neo-bladder. However, many challenges remain for the use of bladder smooth muscle cells (SMCs) as the cell sources. Recent evidences showed that smooth muscle progenitor cells (SPCs) in the peripheral blood have the capacity of differentiating into SMCs, while their use for bladder regeneration hasn't yet been reported...
January 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29317369/mesenchymal-stem-cell-derived-extracellular-matrix-enhances-chondrogenic-phenotype-of-and-cartilage-formation-by-encapsulated-chondrocytes-in-vitro-and-in-vivo
#6
Yuanheng Yang, Hang Lin, He Shen, Bing Wang, Guanghua Lei, Rocky S Tuan
Mesenchymal stem cell derived extracellular matrix (MSC-ECM) is a natural biomaterial with robust bioactivity and good biocompatibility, and has been studied as a scaffold for tissue engineering. In this investigation, we tested the applicability of using decellularized human bone marrow derived MSC-ECM (hBMSC-ECM) as a culture substrate for chondrocyte expansion in vitro, as well as a scaffold for chondrocyte-based cartilage repair. hBMSC-ECM deposited by hBMSCs cultured on tissue culture plastic (TCP) was harvested, and then subjected to a decellularization process to remove hBMSCs...
January 6, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29314764/biomimetic-fetal-rotation-bioreactor-for-engineering-bone-tissues-effect-of-cyclic-strains-on-upregulation-of-osteogenic-gene-expression
#7
Akhilandeshwari Ravichandran, Feng Wen, Jing Lim, Mark Seow Khoon Chong, Jerry K Y Chan, Swee-Hin Teoh
Cells respond to physiological mechanical stresses especially during early fetal development. Adopting a biomimetic approach, it is necessary to develop bioreactor systems to explore the effects of physiologically relevant mechanical strains and shear stresses for functional tissue growth and development. This study introduces a multimodal bioreactor system that allows application of cyclic compressive strains on premature bone grafts that are cultured under biaxial rotation (chamber rotation about two axes) conditions for bone tissue engineering...
January 3, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29299496/effects-of-human-adipose-derived-stem-cells-and-platelet-rich-plasma-on-healing-response-of-canine-alveolar-surgical-bone-defects
#8
Reihaneh Shafieian, Maryam Moghaddam Matin, Amin Rahpeyma, Alireza Fazel, Hamideh Salari Sedigh, Ariane Sadr Nabavi, Halimeh Hassanzadeh, Alireza Ebrahimzadeh-Bideskan
Background: Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone defects. This could offer a great advantage to alternative approaches of bone tissue healing-induced therapies at clinically chair-side procedures...
November 2017: Archives of Bone and Joint Surgery
https://www.readbyqxmd.com/read/29282316/silk-based-multilayered-angle-ply-annulus-fibrosus-construct-to-recapitulate-form-and-function-of-the-intervertebral-disc
#9
Bibhas K Bhunia, David L Kaplan, Biman B Mandal
Recapitulation of the form and function of complex tissue organization using appropriate biomaterials impacts success in tissue engineering endeavors. The annulus fibrosus (AF) represents a complex, multilamellar, hierarchical structure consisting of collagen, proteoglycans, and elastic fibers. To mimic the intricacy of AF anatomy, a silk protein-based multilayered, disc-like angle-ply construct was fabricated, consisting of concentric layers of lamellar sheets. Scanning electron microscopy and fluorescence image analysis revealed cross-aligned and lamellar characteristics of the construct, mimicking the native hierarchical architecture of the AF...
December 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29281943/biological-and-mechanical-factors-promote-the-osteogenesis-of-rabbit-artificial-vertebral-laminae
#10
Linli Li, Xujun Chen, Yiqun He, Youhai Dong
Reconstruction of vertebral laminae without epidural scar formation has been challenging. The success of bone formation depends on the biological and mechanical conditions of the surrounding tissues. In this study, we aimed to investigate the roles of biological and mechanical factors in the osteogenesis of artificial laminae. Mesenchymal stem cells derived from rabbit umbilical cord Wharton's Jelly were induced for osteogenic differentiation for 3 weeks before seeding on the hydroxyapatite-collagen I scaffolds to construct the tissue-engineered laminae (TEL)...
December 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29273785/construction-of-engineered-corpus-cavernosum-with-primary-mesenchymal-stem-cells-in-vitro
#11
Xiaoshuai Xie, Xiaohang Du, Kailin Li, Yuan Chen, Yong Guan, Xiaofei Zhao, Guangzhu Niu, Yun Luan, Denglu Zhang, Chao Sun, Guanghui Cheng, Jue Wang, Qian Xin, Aibing Xue, Peng Wang, Feng Kong, Xiaoli Liu, Hongwei Wang, Yuqiang Liu, Chuan Tian, Mingzhen Yuan, Shuangde Liu, Shengtian Zhao
Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29238978/use-of-autologous-adipose-derived-mesenchymal-stem-cells-for-creation-of-laryngeal-cartilage
#12
Hongji Zhang, Sherry Voytik-Harbin, Sarah Brookes, Lujuan Zhang, Joseph Wallace, Noah Parker, Stacey Halum
OBJECTIVES/HYPOTHESIS: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model. STUDY DESIGN: Basic science experimental design...
December 14, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29235444/contact-guidance-for-cardiac-tissue-engineering-using-3d-bioprinted-gelatin-patterned-hydrogel
#13
Ajay Tijore, Scott Alexander Irvine, Udi Sarig, Priyadarshini Mhaisalkar, Vrushali Baisane, Subbu S Venkatraman
Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior...
December 13, 2017: Biofabrication
https://www.readbyqxmd.com/read/29235057/design-and-assessment-of-a-dynamic-perfusion-bioreactor-for-large-bone-tissue-engineering-scaffolds
#14
Birru Bhaskar, Robert Owen, Hossein Bahmaee, Parcha Sreenivasa Rao, Gwendolen C Reilly
Bioreactors can be used to apply fluid flow in vitro to scaffolds to improve mass transport of media and apply mechanical forces to cells. In this study, we developed and tested an autoclavable, modular perfusion bioreactor suitable for large scaffolds. We investigated the effects of fluid flow induced shear stress (FFSS) on osteogenic differentiation of human embryonic stem cell-derived mesenchymal progenitors (hES-MP cells) cultured on large polyurethane (PU) scaffolds (30 mm diameter × 5 mm thickness) in osteogenesis induction media (OIM)...
December 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#15
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29229621/stem-cell-therapy-for-congenital-heart-disease-a-systematic-review
#16
REVIEW
Diamantis I Tsilimigras, Evangelos K Oikonomou, Demetrios Moris, Dimitrios Schizas, Konstantinos P Economopoulos, Konstantinos S Mylonas
BACKGROUND: Congenital heart disease (CHD) constitutes the most prevalent and heterogeneous group of congenital anomalies. Although surgery remains the gold standard treatment modality, stem cell therapy has been gaining ground as a complimentary or alternative treatment option in certain types of CHD. The aim of this study was to present the existing published evidence and ongoing research efforts on the implementation of stem cell-based therapeutic strategies in CHD. METHODS: A systematic review was conducted by searching Medline, ClinicalTrials...
December 12, 2017: Circulation
https://www.readbyqxmd.com/read/29229365/repair-of-maxillary-cystic-bone-defects-with-mesenchymal-stem-cells-seeded-on-a-cross-linked-serum-scaffold
#17
Luis Miguel Redondo, Verónica García, Beatriz Peral, Alberto Verrier, José Becerra, Ana Sánchez, Javier García-Sancho
Tissue engineering combining cross-linked serum scaffolds with bone-derived mesenchymal stem cells has displayed excellent results for repair of maxillofacial bone defects in animal models, but it had not been tested in humans yet. We present here a pilot clinical trial using autologous bone-derived mesenchymal stem cells (H-MSV) grown in a serum cross-linked scaffold (BioMax) for treatment of maxillary cysts in 9 patients. Cells obtained from alveolar bone were seeded in the BioMax scaffold prepared from autologous serum, expanded under GMP conditions, and subjected to osteogenic differentiation for 3-4 weeks before application...
November 16, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29210278/decellularized-liver-matrix-modified-cryogel-scaffolds-as-potential-hepatocyte-carriers-in-bioartificial-liver-support-systems-and-implantable-liver-constructs
#18
Apeksha Damania, Anupam Kumar, Arun Kumar Teotia, Kimura Haruna, Masamichi Kamihira, Hiroyuki Ijima, Shiv Kumar Sarin, Ashok Kumar
Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise in addressing the issue of donor organ shortage. Decellularization preserves the mechanical integrity, composition, and microvasculature critical for zonation of hepatocytes in the liver. Earlier studies have reported the possibility of repopulating decellularized matrices with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study the versatility of decellularized liver matrix as a substrate coating of 3D cryogel scaffolds...
December 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29208555/hollow-mesoporous-zirconia-delivery-system-for-biomineralization-precursors
#19
Xue-Qing Huang, Hong-Ye Yang, Tao Luo, Cui Huang, Franklin R Tay, Li-Na Niu
Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO2) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility...
December 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29208552/controlling-human-corneal-stromal-stem-cell-contraction-to-mediate-rapid-cell-and-matrix-organization-of-real-architecture-for-3-dimensional-tissue-equivalents
#20
Dev Mukhey, James Phillips, Julie T Daniels, Alvena K Kureshi
The architecture of the human corneal stroma consists of a highly organized extracellular matrix (ECM) interspersed with keratocytes. Their progenitor cells; corneal stromal stem cells (CSSC) are located at the periphery, in the limbal stroma. A highly organized corneal ECM is critical for effective transmission of light but this structure may be compromised during injury or disease, resulting in loss of vision. Re-creating normal organization in engineered tissue equivalents for transplantation often involves lengthy culture times that are inappropriate for clinical use or utilisation of synthetic substrates that bring complications such as corneal melting...
December 2, 2017: Acta Biomaterialia
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