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Stem cell AND seed cells AND tissue engineering

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https://www.readbyqxmd.com/read/28804565/thrombospondin-1-inhibits-ossification-of-tissue-engineered-cartilage-constructed-by-adscs
#1
Aiguo Xie, Jixin Xue, Gan Shen, Lanjun Nie
Cartilage tissue engineering provides a new method in the treatment of cartilage defects, and adipose derived stem cells seem to be an ideal seed cell in cartilage tissue engineering because of its characteristics. However, ossification after in vivo implantation of tissue engineered cartilage remains a challenge. Thrombospondin-1 which has been reported to have an inhibitory effect on angiogenesis, may play an important role in inhibiting the ossification of tissue engineered cartilage constructed by adipose derived stem cells...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28802849/silk-fibroin-chitosan-scaffold-with-tunable-properties-and-low-inflammatory-response-assists-the-differentiation-of-bone-marrow-mesenchymal-stem-cells
#2
Da-Wei Li, Xiaohua Lei, Feng-Li He, Jin He, Ya-Li Liu, Ya-Jing Ye, Xudong Deng, Enkui Duan, Da-Chuan Yin
The physical and chemical properties of the scaffold are known to play important roles in three-dimensional (3D) cell culture, which always determine the cellular fate or the results of implantation. To control these properties becomes necessary for meeting the requirements of a variety of tissue engineering applications. In this study, a series of silk fibroin/chitosan (SF/CS) scaffolds with tunable properties were prepared using freeze-drying method, and the rat bone marrow-derived mesenchymal stem cells (BM-MSCs) were seeded in these scaffolds to evaluate their availability of use in tissue engineering...
August 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28792664/collagen-coated-nano-electrospun-pcl-seeded-with-human-endometrial-stem-cells-for-skin-tissue-engineering-applications
#3
Shiva Sharif, Jafar Ai, Mahmoud Azami, Javad Verdi, Mohammad Ali Atlasi, Sadegh Shirian, Ali Samadikuchaksaraei
Human endometrial stem cells (hEnSCs) are known as an attractive source of stem cells for regenerative medicine. hEnSCs are easily isolated and are capable of repairing uterine through their strong ability of creating new capillaries. In this study, a three-dimensional (3D) nanofibrous polycaprolactone (PCL)/collagen scaffold was fabricated and characterized in order to be applied as a new approach for skin reconstruction. Furthermore, the behavior of hEnSCs on this scaffold was investigated. First, a PCL 3D scaffold was constructed using electrospinning technique...
August 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28776691/three-dimensionally-printed-polyetherketoneketone-scaffolds-with-mesenchymal-stem-cells-for-the-reconstruction-of-critical-sized-mandibular-defects
#4
Michael G Roskies, Dongdong Fang, Mohamed-Nur Abdallah, Andre M Charbonneau, Navi Cohen, Jack O Jordan, Michael P Hier, Alex Mlynarek, Faleh Tamimi, Simon D Tran
OBJECTIVE: Additive manufacturing offers a tailored approach to tissue engineering by providing anatomically precise scaffolds onto which stem cells and growth factors can be supplied. Polyetherketoneketone (PEKK), an ideal candidate biomaterial, is limited by a poor implant-bone interface but can be functionalized with adipose-derived stem cells (ADSC) to promote integration. This in vivo study examined the interaction of a three-dimensional printed PEKK/ADSC implant within the critical-sized mandibular defect in a rabbit model...
August 4, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28774013/additive-manufacturing-of-patient-customizable-scaffolds-for-tubular-tissues-using-the-melt-drawing-method
#5
Yu Jun Tan, Xipeng Tan, Wai Yee Yeong, Shu Beng Tor
Polymeric fibrous scaffolds for guiding cell growth are designed to be potentially used for the tissue engineering (TE) of tubular organs including esophagi, blood vessels, tracheas, etc. Tubular scaffolds were fabricated via melt-drawing of highly elastic poly(l-lactide-co-ε-caprolactone) (PLC) fibers layer-by-layer on a cylindrical mandrel. The diameter and length of the scaffolds are customizable via 3D printing of the mandrel. Thickness of the scaffolds was varied by changing the number of layers of the melt-drawing process...
November 3, 2016: Materials
https://www.readbyqxmd.com/read/28768930/curcumin-inhibits-chondrocyte-hypertrophy-of-mesenchymal-stem-cells-through-ihh-and-notch-signaling-pathways
#6
Zhen Cao, Ce Dou, Shiwu Dong
Using tissue engineering technique to repair cartilage damage caused by osteoarthritis is a promising strategy. However, the regenerated tissue usually is fibrous cartilage, which has poor mechanical characteristics compared to hyaline cartilage. Chondrocyte hypertrophy plays an important role in this process. Thus, it is very important to find out a suitable way to maintain the phenotype of chondrocytes and inhibit chondrocyte hypertrophy. Curcumin deriving from turmeric was reported with anti-inflammatory and anti-tumor pharmacological effects...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28762960/zinc-and-strontium-based-phosphate-glass-beads-a-novel-material-for-bone-tissue-engineering
#7
Mustafa Alqaysi, Alaa Aldaadaa, Nicky Mordan, Rishma Shah, Jonathan C Knowles
Degradable phosphate-based glasses that contain strontium, zinc and calcium were investigated to examine its function as an osteoconductive material. Glass beads of the general formula of (P2O5)-(Na2O)-(TiO2)-(CaO)-(SrO) or (ZnO) were prepared by melt quench technique followed by milling and spheroidisation. Glass bead size distribution was initially measured by SEM. Then, some of these samples were immersed in deionized water to evaluate both the surface changes and measure the ion release rate, whereas other glass beads samples were incubated in culture media to determine pH changes...
August 1, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28756760/spray-delivery-of-intestinal-organoids-to-reconstitute-epithelium-on-decellularized-native-extracellular-matrix
#8
Dana M Schwartz, Meryem O Pehlivaner Kara, Allan Goldstein, Harold C Ott, Adam Ekenseair
The native extracellular matrix (ECM) serves as a unique platform for tissue engineering because it provides an organ-specific scaffold in terms of both matrix composition and tissue architecture. However, efficacious cell-seeding techniques for recellularizing the ECM constructs with appropriate cell types to restore biologic function remain under development. In this study, the impact of spraying as a seeding technique for repopulation of decellularized small intestine was investigated. In a series of experiments, CaCo-2 cells was first used to investigate the effect of spray device type and pressure on cell viability and to optimize parameters for seeding intestinal epithelial cells...
July 29, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28752802/three-dimensional-wet-electrospun-poly-lactic-acid-multi-wall-carbon-nanotubes-scaffold-induces-differentiation-of-human-menstrual-blood-derived-stem-cells-into-germ-like-cells
#9
Hossein Eyni, Sadegh Ghorbani, Reza Shirazi, Leila Salari Asl, Shahram P Beiranvand, Masoud Soleimani
Infertility caused by the disruption or absence of germ cells is a major and largely incurable medical problem. Germ cells (i.e., sperm or egg) play a key role in the transmission of genetic and epigenetic information across generations. Generation of gametes derived in vitro from stem cells hold promising prospects which could potentially help infertile men and women. Menstrual blood-derived stem cells are a unique stem cell source. Evidence suggests that menstrual blood-derived stem cells exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28752541/functionalization-of-porous-bcp-scaffold-by-generating-cell-derived-extracellular-matrix-from-rat-bone-marrow-stem-cells-culture-for-bone-tissue-engineering
#10
Boram Kim, Reiza Ventura, Byong-Taek Lee
The potential of decellularized cell-derived ECM deposited on biphasic calcium phosphate (BCP) scaffold for bone tissue engineering was investigated. Rat derived bone marrow mesenchymal stem cells (RBMSCs) were cultured on porous BCP scaffolds for 3 weeks and decellularized with two different methods (freeze-thaw (F/T) or sodium dodecyl sulfate (SDS)). The decellularized ECM deposited scaffolds (dECM-BCP) were characterized through scanning electron microscopy, energy dispersive X-ray spectrometer, and confocal microscopy...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28747099/in-vivo-models-for-the-evaluation-of-the-osteogenic-potency-of-bone-substitutes-seeded-with-mesenchymal-stem-cells-of-human-origin-a-concise-review-special-issue-animal-models-in-tissue-engineering
#11
Fabian Westhauser, Anne-Sophie Senger, Bruno Reible, Arash Moghaddam
Research concerning bone substitutes is one of the most challenging fields in orthopedic research and has a high clinical relevance, especially since the currently available bone substitutes are limited in their osteostimulative capabilities. In-vitro models for the evaluation of the properties of bone substitutes allow the use of human mesenchymal stem cells (hMSC) seeded onto scaffolds, but suffer from the lack of a physiological environment for those cells. Most in-vivo models include the use of non-human MSC and are therefore lacking in clinical relevance...
July 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28747097/tissue-engineering-strategies-to-improve-osteogenesis-in-the-juvenile-swine-alveolar-cleft-model
#12
Montserrat Caballero, Donna C Jones, Zhengyuan Shan, Sajjad Soleimani, John A van Aalst
Alveolar <b>(gumline)</b> clefts are the most common congenital bone defect in humans, affecting 1 in 700 live births. Treatment <b>to repair these bony</b> defects relies on autologous, <b>cancellous</b> bone transfer from the iliac crest. This harvest requires a second surgical site <b>with increased </b>surgical time associated with potential complications, <b>while providing only limited cancellous bone</b>. Improvements in treatment protocols that avoid these limitations would be beneficial to patients with clefts and other craniofacial bone defects...
July 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28746277/auricular-tissue-engineering-using-osteogenic-differentiation-of-adipose-stem-cells-with-small-intestine-submucosa
#13
Chih-Hsun Lin, I-Chen Yang, Chi-Han Tsai, Hsu-Wei Fang, Hsu Ma
BACKGROUND: Ear reconstruction remains a challenge for plastic surgeons. A tissue-engineering approach could provide another route for obtaining shape maintenance in neoauricular tissue. METHODS: The authors designed a novel tissue-engineering auricular construct by culturing human adipose stem cells, which differentiated into osteocytes but not chondrocytes, in small intestine submucosa scaffolds. The authors evaluated cell growth potential and mechanical properties...
August 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28746081/synergism-of-electrospun-nanofibers-and-pulsed-electromagnetic-field-on-osteogenic-differentiation-of-induced-pluripotent-stem-cells
#14
Abdolreza Ardeshirylajimi, Arash Khojasteh
According to the current therapies failure for bone fractures and lesions, tissue engineering showed a great potential to help solve these challenges. Because the use of growth factors is very limited in the clinic, it could be very useful that could be introducing an alternative to it. Extremely low frequency pulsed electromagnetic fields (PEMF, 1 mT, 50 Hz) were used for achieving this aim. The PEMF potential in combination with electrospun polycaprolactone (PCL) nanofibers was used to investigate the osteogenic potential of human induced pluripotent stem cells (iPSCs)...
July 25, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28743897/the-healing-of-alveolar-bone-defects-with-novel-bio-implants-composed-of-ad-bmp9-transfected-rdfcs-and-cha-scaffolds
#15
Li Nie, Xia Yang, Liang Duan, Enyi Huang, Zhou Pengfei, Wenping Luo, Yan Zhang, Xingqi Zeng, Ye Qiu, Ting Cai, Conghua Li
Cells, scaffolds, and growth factors play important roles in bone regeneration. Bone morphogenetic protein 9 (BMP9), a member of BMP family, could facilitate osteogenesis by regulating growth factors and promoting angiogenesis. Similar to other stem cells, rat dental follicle stem cells (rDFCs), the precursor cells of cementoblasts, osteoblasts and periodontal ligament cells, can self-renew and exhibit multipotential capacity. Coralline hydroxyapatite (CHA) has good biocompatibility and conductivity required for bone tissue engineering...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28740258/mechanically-stable-fibrin-scaffolds-promote-viability-and-induce-neurite-outgrowth-in-neural-aggregates-derived-from-human-induced-pluripotent-stem-cells
#16
Meghan Robinson, Sarah Douglas, Stephanie Michelle Willerth
Recent work demonstrated that 3D fibrin scaffolds function as an effective substrate for engineering tissues from pluripotent stem cells. However, the rapid degradation rate of fibrin remains a major limitation when differentiating human pluripotent stem cells for tissue engineering applications. The addition of crosslinking agents, such as genipin, during the polymerization process increases scaffold stability while decreasing the degradation rate of fibrin. Genipin crosslinking alters the physical characteristics of the fibrin scaffolds, which influences the behaviour of the differentiating cells seeded inside...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28722162/cyanidin-suppresses-autophagic-activity-regulating-chondrocyte-hypertrophic-differentiation
#17
Zhen Cao, Song Huang, Ce Dou, Qiang Xiang, Shiwu Dong
Cartilage is a kind of special connective tissue which does not contain neither blood vessels nor lymphatics and nerves. Therefore, the damage in cartilage is difficult to be repaired spontaneously. Constructing tissue engineered cartilage provides a new technique for cartilage repairing. Mesenchymal stem cells (MSCs) possess a unique capability of self-renew and can differentiate into pre-chondrocytes which are frequently applied as seed cells in tissue engineering. However, in regenerated cartilage the chondrocytes derived from MSCs can hardly maintain homeostasis and preferentially present hypertrophic like phenotype...
July 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28715029/anisotropic-microfibrous-scaffolds-enhance-the-organization-and-function-of-cardiomyocytes-derived-from-induced-pluripotent-stem-cells
#18
Maureen Wanjare, Luqia Hou, Karina H Nakayama, Joseph J Kim, Nicholas P Mezak, Oscar J Abilez, Evangeline Tzatzalos, Joseph C Wu, Ngan F Huang
Engineering of myocardial tissue constructs is a promising approach for treatment of coronary heart disease. To engineer myocardial tissues that better mimic the highly ordered physiological arrangement and function of native cardiomyocytes, we generated electrospun microfibrous polycaprolactone scaffolds with either randomly oriented (14 μm fiber diameter) or parallel-aligned (7 μm fiber diameter) microfiber arrangement and co-seeded the scaffolds with human induced pluripotent stem cell-derived cardiomyocytes (iCMs) and endothelial cells (iECs) for up to 12 days after iCM seeding...
July 25, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28714856/use-of-human-aortic-extracellular-matrix-as-a-scaffold-for-construction-of-patient-specific-tissue-engineered-vascular-patch
#19
Liping Gao, Mingjun Du, Jingjing Lv, Sebastian Schmull, Ritai Huang, Jun Li
Abstract Synthetic or biologic materials are usually used to repair vascular malformation in congenital heart defects; however, non-autologous materials show both mismatch compliance and antigenicity, as well as a lack of recellularization on its surface. Here, we constructed a tissue-engineered vascular patch (TEVP) using decellularized extracellular matrix (ECM) scaffold obtained from excised human aorta during surgery, which was seeded with patient-derived bone marrow CD34-positive (CD34+) progenitor cells...
July 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#20
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
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