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https://www.readbyqxmd.com/read/29050265/reactive-oxygen-species-dependent-phosphorylation-of-the-liver-kinase-b1-amp-activated-protein-kinase-acetyl-coa-carboxylase-signaling-is-critically-involved-in-apoptotic-effect-of-lambertianic-acid-in-hepatocellular-carcinoma-cells
#1
Arong Jeong, Ju-Ha Kim, Hyo-Jung Lee, Sung-Hoon Kim
Though lambertianic acid (LA) is reported to have hypolipidemic activity in liver, its underlying anticancer mechanism is poorly understood so far. Thus, in the present study, apoptotic mechanism of LA was elucidated in HepG2 and SK-Hep1 hepatocellular carcinoma (HCC) cells. Here LA increased cytotoxicity, sub-G1 population and Annexin V/PI positive cells in two HCC cells. Also, LA cleaved caspase-3 and poly(ADP-ribose) polymerase (PARP), activated phosphorylation of liver kinase B1 (LKB1)/AMP activated protein kinase (AMPK)/ acetyl-CoA carboxylase (ACC) pathway and also suppressed antiapoptotic proteins such as phosphorylation of Akt/ mammalian target of rapamycin (mTOR) and the expression of B cell lymphoma-2 (Bcl-2)/ B-cell lymphoma-extra large (Bcl-xL) and cyclooxygenase-2 (COX-2) in two HCC cells...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050034/mir-29b-regulates-oxidative-stress-by-targeting-sirt1-in-ovarian-cancer-cells
#2
Meng Hou, Xiaohang Zuo, Chen Li, Yan Zhang, Yue Teng
BACKGROUND: Metabolic abnormalities are frequently observed in multiple malignancies including epithelial ovarian cancer (EOC), among which imbalance between generation and elimination of reactive oxygen species (ROS) plays a critical role in EOC onset and progression. Here we investigated the role of miR-29b, a well-established tumor-suppressor miRNA in metabolic regulation of EOC cells. METHODS: cell viability and apoptosis in miR-29b inhibited and over-expressed EOC cells were evaluated by CCK8 and Annexin V-FITC/PI assays...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29045825/cerenkov-luminescence-imaging-on-evaluation-of-early-response-to-chemotherapy-of-drug-resistant-gastric-cancer
#3
Muhan Liu, Sheng Zheng, Xiaojun Zhang, Hongbo Guo, Xiaojing Shi, Xiaoyu Kang, Yawei Qu, Zhenhua Hu, Jie Tian
Apoptosis imaging enables a timely and specific assessment of treatment response in cancer patients. In this study, we applied a probe for positron emission tomography (PET), served as an optical biomaterial emitting Cerenkov photons, to in vivo optical imaging of tumor apoptosis, in order to evaluate early response to chemotherapy of drug-resistant gastric cancer. (68)Ga-DOTA-Annexin V was prepared as the apoptosis targeting probe. Wild type human gastric adenocarcinoma cell line SGC7901/WT and drug vincristine-resistant variant SGC7901/VCR were used to establish normal and vincristine-resistant xenograft to simulate treatment decision situation...
October 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29045759/the-effect-of-hydroxychloroquine-on-haemostasis-complement-inflammation-and-angiogenesis-in-patients-with-antiphospholipid-antibodies
#4
Karen Schreiber, Karen Breen, Kiran Parmar, Jacob H Rand, Xiao-Xuan Wu, Beverley J Hunt
Objectives: HCQ has been described as having a beneficial effect in patients with APS but its mechanism of action is unclear. We hypothesized that HCQ may have effects on subnormal angiogenesis, inflammation and haemostatic biomarkers seen in APS. The aim of our study was to assess laboratory markers [annexin A5 (AnxA5) anticoagulant activity, tissue factor (TF) levels, thromboelastography (TEG), CRP, Bb, C3a and VEGF] in HCQ-naïve patients with aPL at baseline and after commencing HCQ...
October 17, 2017: Rheumatology
https://www.readbyqxmd.com/read/29045486/thiamine-deficiency-activates-hypoxia-inducible-factor-1%C3%AE-to-facilitate-pro-apoptotic-responses-in-mouse-primary-astrocytes
#5
Kristy Zera, Jason Zastre
Thiamine is an essential enzyme cofactor required for proper metabolic function and maintenance of metabolism and energy production in the brain. In developed countries, thiamine deficiency (TD) is most often manifested following chronic alcohol consumption leading to impaired mitochondrial function, oxidative stress, inflammation and excitotoxicity. These biochemical lesions result in apoptotic cell death in both neurons and astrocytes. Comparable histological injuries in patients with hypoxia/ischemia and TD have been described in the thalamus and mammillary bodies, suggesting a congruency between the cellular responses to these stresses...
2017: PloS One
https://www.readbyqxmd.com/read/29044433/pregnancy-induced-changes-in-metabolome-and-proteome-in-ovine-uterine-flushings
#6
Jared J Romero, Bethany E Liebig, Corey D Broeckling, Jessica E Prenni, Thomas R Hansen
Mass spectrometry (MS) approaches were used herein to identify metabolites and proteins in uterine flushings (UF) that may contribute to nourishing the conceptus. Ovine uteri collected on Day 12 of the estrous cycle (n = 5 ewes exposed to vasectomized ram) or Days 12 (n = 4), 14 (n = 5), or 16 (n = 5) of pregnancy (bred with fertile ram) were flushed using buffered saline. Metabolites were extracted using 80% methanol and profiled using ultraperformance liquid chromatography (LC) tandem mass spectrometry. The proteome was examined by digestion with trypsin, followed by the analysis of peptides with LC-MS/MS...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29042989/microrna-22-may-promote-apoptosis-and-inhibit-the-proliferation-of-hypertrophic-scar-fibroblasts-by-regulating-the-mitogen-activated-protein-kinase-kinase-extracellular-signal-regulated-kinase-p21-pathway
#7
Shihua Dong, Yanfeng Sun
Hypertrophic scarring (HS) is a common skin disorder that occurs during the wound healing process, and the pathogenesis of HS remains unclear. Increasing evidence indicated that specific microRNAs (miRs) may be involved in the onset and progression of HS. In the present study, the association between miR-22 and HS was investigated. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to examine the expression of miR-22 in 30 HS and matched normal skin tissues. In addition, human hypertrophic scar fibroblasts (HSFBs) were cultured and transfected with miR-22 mimics, and MTT and Annexin V apoptosis assays were performed to investigate the role of miR-22 in the proliferation and apoptosis of the human HSFBs...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042932/cardioprotective-effect-of-notch-signaling-on-the-development-of-myocardial-infarction-complicated-by-diabetes-mellitus
#8
Fang Wu, Bo Yu, Xu Zhang, Yuelan Zhang
The present study aimed to elucidate the role of Notch signaling in the development of myocardial infarction (MI) concomitant with diabetes in vivo and in vitro and evaluated the therapeutic effect of the Notch signaling in vitro. Streptozotocin-induced diabetic rats were subjected to 25 min of ischemia and 2 h of reperfusion. Cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) isoenzyme levels were detected. Infarct size was measured by 2,3,5-triphenyltetrazolium chloride staining. Myocardial apoptosis and fibrosis were examined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and Masson Trichrome staining, respectively...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29041990/piceatannol-induced-apoptosis-through-up-regulation-of-microrna-181a-in-melanoma-cells
#9
Maotao Du, Zhong Zhang, Tao Gao
BACKGROUND: Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells...
October 17, 2017: Biological Research
https://www.readbyqxmd.com/read/29040813/developmental-neurotoxic-effects-of-a-low-dose-of-tce-on-3d-neurosphere-system
#10
Mohamed Elsaid Abdraboh, Sherif H Abdeen, Mohamed Salama, Mahmoud Elhussiny, Yasser M El-Sherbini, Nada M Eldeen
Trichloroethylene (TCE) is one of the industrial toxic products that ever-present in air, soil and water. Several studies illustrated the toxicity of high doses of TCE on several organs. This study aims to determine the toxic effect of a low dose of TCE on development of rat neural stem cells (NSCs). Rat pups brains were collected, minced and harvested cells were cultured in the presence of neural growth factors B27/N8 to develop neurospheres. Afterwards, cells were subjected to a dose of 1µM TCE for 1 and 2 weeks...
October 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29040664/coumestrol-induces-mitochondrial-dysfunction-by-stimulating-ros-production-and-calcium-ion-influx-into-mitochondria-in-human-placental-choriocarcinoma-cells
#11
Whasun Lim, Changwon Yang, Muhah Jeong, Fuller W Bazer, Gwonhwa Song
STUDY QUESTION: Does coumestrol inhibit proliferation of human placental choriocarcinoma cells? SUMMARY ANSWER: Coumestrol promotes cell death in the choriocarcinoma cells by regulating ERK1/2 MAPK and JNK MAPK signaling pathways and through disruption of Ca2+ and ROS homeostasis. WHAT IS KNOWN ALREADY: A number of patients who suffer from choriocarcinomas fail to survive due to delayed diagnosis or a recurrent tumor and resistance to traditional chemotherapy using platinum-based agents and methotrexate...
October 11, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29040392/%C3%AE-cypermethrin-and-its-metabolite-3-phenoxybenzoic-acid-exhibit-immunotoxicity-in-murine-macrophages
#12
Xia Wang, Bingnan He, Baida Kong, Lai Wei, Rong Wang, Chenqian Zhou, Yiyan Shao, Jiajia Lin, Yuanxiang Jin, Zhengwei Fu
β-Cypermethrin (β-CYP), one of most important pyrethroids, is widely used to control insects, and has been detected in organisms, including human. Pyrethroids have been shown to pose neurotoxicity, hepatotoxicity, endocrine disruption and reproductive risks in mammals. However, research in immunotoxicity of pyrethroids, especially their metabolites, is limited. A common metabolite of pyrethroids is 3-phenoxybenzoic acid (3-PBA) in mammals. Thus, in this study, we evaluated the immunotoxicity of β-CYP and 3-PBA in mouse macrophages, RAW 264...
October 11, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29039593/hyperthermia-with-different-temperatures-inhibits-proliferation-and-promotes-apoptosis-through-the-egfr-stat3-pathway-in-c6-rat-glioma-cells
#13
Yao-Dong Chen, Yu Zhang, Tian-Xiu Dong, Yu-Tong Xu, Wei Zhang, Ting-Ting An, Peng-Fei Liu, Xiu-Hua Yang
Malignant gliomas are a group of aggressive neoplasms among human cancers. The curative effects of current treatments are finite for improving the prognosis of patients. Hyperthermia (HT) is an effective treatment for cancers; however, the effects of HT with different temperatures in treatment of MG and relevant mechanisms remain unclear. MTT assay and Annexin V‑fluorescein isothiocyanate/propidium iodide staining were used for investigating the proliferation and apoptosis of C6 cells, respectively. Western blotting was applied to detect the expression of proteins...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039582/curcumol-inhibits-the-proliferation-of-gastric-adenocarcinoma-mgc-803-cells-via-downregulation-of-idh1
#14
Shilei Zang, Qiling Tang, Fangrui Dong, Huan Liu, Lili Li, Fang Guo, Xuediao Pan, Haorong Lin, Weibin Zeng, Zhaoxu Cai, Qibin Zhong, Ning Zang, Linquan Zang
Curcumol, a polyphenol compound derived from the rhizome of Curcuma, has been established as an antitumor compound against multiple types of cancer, including gastric (GC), lung, liver and breast cancer. However, the molecular mechanisms undelying its anticancer activity in GC are still unclear. In this study, the antitumor efficacy of curcumol was ascertained in human gastric adenocarcinoma MGC-803 cells. An MTT assay was used to assess the viability of the MGC-803 cells treated by curcumol. The results of the Annexin V/propidium iodide (PI) staining followed by fluorescence activated cell sorting (FACS) analysis demonstrated that the cell cycle was arrested in the G2/M phase by curcumol...
October 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29039553/proteomic-analysis-of-serum-deprivation-in-tongue-squamous-cell-carcinoma
#15
Junfeng Zhang, Wei Dong, Yufen Meng, Miao Jiang, Zhen Zhan
The occurrence of tongue squamous cell carcinoma (TSCC) is closely correlated with serum components; however, the detailed mechanism remains to be fully elucidated. Proteomic analysis contributed to the discovery of potential biomarkers and provided an insight into TSCC at a molecular level. The present study investigated the effect of serum deprivation on the Tca‑8113 TSCC cell line through protein profiling using two‑dimensional gel electrophoresis and mass spectrometry, with the aim of improving TSCC diagnosis...
October 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29035889/inhibition-of-suicidal-erythrocyte-death-by-volasertib
#16
Abdulla Al Mamun Bhuyan, A K M Ashiqul Haque, Itishri Sahu, Hang Cao, Michael S D Kormann, Florian Lang
BACKGROUND/AIMS: The Polo-like kinase 1 (Plk1) inhibitor volasertib is used in the treatment of malignancy. Volasertib is partially effective by triggering suicidal death or apoptosis of tumor cells. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal cell death or eryptosis, which is characterized by cell membrane scrambling with phosphatidylserine translocation to the cell surface and by cell shrinkage. Stimulators of eryptosis include energy depletion, hyperosmotic shock, oxidative stress and excessive increase of cytosolic Ca2+ activity ([Ca2+]i)...
October 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29035827/ginsenoside-rg3-enhances-the-anti-proliferative-activity-of-erlotinib-in-pancreatic-cancer-cell-lines-by-downregulation-of-egfr-pi3k-akt-signaling-pathway
#17
Jin Jiang, Zuguo Yuan, Yiqing Sun, Yuan Bu, Wenfeng Li, Zhenghua Fei
Erlotinib has shown activity in the management of pancreatic cancer. However, the benefit of EGFR blockade is limited due to EGFR independent PI3K/Akt signaling pathway. Studies have reported that Ginsenoside Rg3 strongly inhibited PI3K-Akt signaling pathway of many carcinomas. We aimed to investigate the activity of Ginsenoside Rg3 to sensitize erlotinib in treating pancreatic cancer in vitro and in vivo. Human pancreatic cancer cell lines BxPC-3 and AsPC-1 were used. Cell proliferation and colony formation assay, Annexin V/PI apoptosis analysis, Western blot analysis, immunohistochemistry and in vivo study were carried out...
October 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29034444/mage-a11-expression-contributes-to-cisplatin-resistance-in-head-and-neck-cancer
#18
Stefan Hartmann, Leonie Zwick, Mario J J Scheurer, Andreas R Fuchs, Roman C Brands, Axel Seher, Hartmut Böhm, Alexander C Kübler, Urs D A Müller-Richter
OBJECTIVE: The objective of this study is to investigate the roles of melanoma-associated antigens (MAGEs) in the cisplatin treatment of head and neck cancer. MATERIALS AND METHODS: We assessed the efficacy of cisplatin in a set of four head and neck cancer cell lines using a crystal violet assay. The MAGE-A expression in all cell lines was measured with RT-qPCR. The correlation between MAGE-A expression and cisplatin efficacy was investigated using Spearman's correlation analysis...
October 15, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29031204/ginkgetin-inhibits-growth-of-breast-carcinoma-via-regulating-mapks-pathway
#19
Jun Cao, Chuang Tong, Yanyan Liu, Jianguo Wang, Xiaoyan Ni, Mao-Ming Xiong
The purpose of present study was to investigate anti-tumor activity of Ginkgetin (GK) and its mechanism of action in breast cancer. The effects of GK on growth of human breast cancer cell lines MDA-MB-231, BT-474 and MCF-7 were examined by MTT assay. Cells apoptosis in MCF-7 cells were analyzed by TUNEL staining and annexin-V and propidium iodide double staining. The effects of GK on expression of apoptotic associated proteins and mitogen-activated protein kinases (MAPKs) were determined by western blotting...
October 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29031200/oleanolic-acid-methyl-ester-a-novel-cytotoxic-mitocan-induces-cell-cycle-arrest-and-ros-mediated-cell-death-in-castration-resistant-prostate-cancer-pc-3-cells
#20
Noha Abdelmageed, Samy A F Morad, Ashraf A Elghoneimy, Tatiana Syrovets, Thomas Simmet, Hesham El-Zorba, Hossney A El-Banna, Myles Cabot, Magdy I Abdel-Aziz
Oleanolic acid derivatives exhibit potent anticancer activities against numerous types of cancer. However, the antitumor activity of oleanolic acid methylester (OAME), an oleanolic acid derivative, against prostate cancer has not been studied. Hence, the present work was conducted to study the anticancer activities of OAME. Viability assay showed that treatment of cancer cells with OAME induced a significant cell death in concentration- and time-dependent manner. Of note, OAME displayed a selective cytotoxicity against cancer cells compared to normal epithelial cells...
October 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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