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https://www.readbyqxmd.com/read/28635396/microrna-34a-inhibits-tumor-invasion-and-metastasis-in-osteosarcoma-partly-by-effecting-c-iap2-and-bcl-2
#1
Jie Wen, Yan-Kun Zhao, Yan Liu, Jin-Feng Zhao
Osteosarcoma is a common primary malignant bone tumor that occurs mainly in children and adolescents. Recent evidence has demonstrated that miR-34a is involved in the invasion and metastasis of osteosarcoma. This study aims to explore the effect of biological behavior of miR-34a on osteosarcoma. First, we collect osteosarcoma and adjacent specimens, and the relative expression of miR-34a and C-IAP2 messenger RNA was quantitated by real-time polymerase chain reaction. Furthermore, miR-34a stimulant is synthesized and transfected onto osteosarcoma MG-63 cells...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28634693/rhepo-enhances-cellular-anti-oxidant-capacity-to-protect-long-term-cultured-aging-primary-nerve-cells
#2
Huqing Wang, Jiaxin Fan, Mengyi Chen, Qingling Yao, Zhen Gao, Guilian Zhang, Haiqin Wu, Xiaorui Yu
Erythropoietin (EPO) may protect the nervous system of animals against aging damage, making it a potential anti-aging drug for the nervous system. However, experimental evidence from natural aging nerve cell models is lacking, and the efficacy of EPO and underlying mechanism of this effect warrant further study. Thus, the present study used long-term cultured primary nerve cells to successfully mimic the natural aging process of nerve cells. Starting on the 11th day of culture, cells were treated with different concentrations of recombinant human erythropoietin (rhEPO)...
June 21, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28634253/role-of-autophagy-in-tumor-necrosis-factor-%C3%AE-induced-apoptosis-of-osteoblast-cells
#3
Liwen Zheng, Wanchun Wang, Jiangdong Ni, Xinzhan Mao, Deye Song, Tang Liu, Jianwei Wei, Zhou Huaying
The aim of this study is to investigate the role of tumor necrosis factor-α (TNF-α) in apoptosis and autophagy of mouse osteoblast MC3T3-E1 cells, as well as the crosstalk between autophagy and apoptosis. Mouse osteoblast MC3T3-E1 cells were cultured in vitro and treated with 5-fluorouracil (5-FU), rapamycin, 3-methyl adenine (3-MA) and TNF-α either alone or in combination, respectively. MTT assays were used to monitor the cell viability upon different treatments. Annexin-V-FITC/propidium iodide (PI) staining was used to detect the apoptotic rate of osteoblasts...
June 20, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/28633130/synthesis-screening-and-pro-apoptotic-activity-of-novel-acyl-spermidine-derivatives-on-human-cancer-cell-lines
#4
Syed Shoeb Razvi, Hany Choudhry, Said Salama Moselhy, Taha Abduallah Kumosani, Mohammed Nihal Hasan, Mazin A Zamzami, Khalid Omer Abualnaja, Abdulrahman Labeed Al-Malki, Mahmoud Alhosin, Tadao Asami
The polyamines putrescine, spermidine, and spermine are polycationic, alkyl polyamines which play a significant role in eukaryotic cell proliferation. The polyamine metabolism and function are dysregulated in tumor cells making them an attractive therapeutic target by employing polyamine analogs. These analogs have a high degree of similarity with the structure of polyamines but not with their function. Multidrug resistance is a major factor in the failure of many chemotherapeutic drugs which necessitates further research and exploration of better novel alternatives...
June 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28632731/cd47-overexpression-is-associated-with-decreased-neutrophil-apoptosis-phagocytosis-and-poor-prognosis-in-non-small-cell-lung-cancer-patients
#5
Lourdes Barrera, Edgar Montes-Servín, Juan-Manuel Hernandez-Martinez, María de Los Ángeles García-Vicente, Elizabeth Montes-Servín, Marytere Herrera-Martínez, José C Crispín, José Rafael Borbolla-Escoboza, Oscar Arrieta
BACKGROUND: Non-small-cell lung cancer (NSCLC) patients often exhibit neutrophilia, which has been associated with poor clinical outcomes. However, the mechanisms that lead to neutrophilia have not been fully established. CD47 is an antiphagocytic molecule that promotes neutrophil recruitment. METHODS: Blood was collected from 50 treatment-naive patients with advanced NSCLC and from 25 healthy subjects. The frequency of CD66b(+) cells and the expression of CD47 were determined by flow cytometry...
June 20, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28631624/printing-induced-cell-injury-evaluation-during-laser-printing-of-3t3-mouse-fibroblasts
#6
Zhengyi Zhang, Wenxuan Chai, Ruitong Xiong, Lei Zhou, Yong Huang
Three-dimensional bioprinting has emerged as a promising solution for the freeform fabrication of living cellular constructs, which can be used for tissue/organ transplantation and tissue models. During bioprinting, some living cells are unavoidably injured and may become necrotic or apoptotic cells. This study aims to investigate the printing-induced cell injury and evaluates injury types of post-printing cells using the annexin V/7-aminoactinomycin D and FAM-DEVD-FMK/propidium iodide assays during laser printing of NIH 3T3 mouse fibroblasts...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28630342/autoantibody-profiling-on-a-plasmonic-nano-gold-chip-for-the-early-detection-of-hypertensive-heart-disease
#7
Xiaoyang Li, Tatiana Kuznetsova, Nicholas Cauwenberghs, Matthew Wheeler, Holden Maecker, Joseph C Wu, Francois Haddad, Hongjie Dai
The role of autoimmunity in cardiovascular (CV) diseases has been increasingly recognized. Autoimmunity is most commonly examined by the levels of circulating autoantibodies in clinical practices. Measurement of autoantibodies remains, however, challenging because of the deficiency of reproducible, sensitive, and standardized assays. The lack of multiplexed assays also limits the potential to identify a CV-specific autoantibody profile. To overcome these challenges, we developed a nanotechnology-based plasmonic gold chip for autoantibody profiling...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-pqs-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#8
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonas aeruginosa (PA) causes lung infection in patients with cystic fibrosis (CF). Pseudomonas Quinolone Signal (PQS) is a secreted PA virulence factor that contributes to the pathogenicity of PA. We were able to detect PQS in sputum samples from CF patients infected with PA, but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial (NHBE)) and macrophages (J774A...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28629465/natural-igm-antibodies-that-bind-neoepitopes-exposed-as-a-result-of-spinal-cord-injury-drive-secondary-injury-by-activating-complement
#9
Aarti Narang, Fei Qiao, Carl Atkinson, Hong Zhu, Xiaofeng Yang, Liudmila Kulik, V Michael Holers, Stephen Tomlinson
BACKGROUND: Natural IgM antibodies (Abs) function as innate immune sensors of injury via recognition of neoepitopes expressed on damaged cells, although how this recognition systems function following spinal cord injury (SCI) exposes various neoepitopes and their precise nature remains largely unknown. Here, we investigated the role of two natural IgM monoclonal Abs (mAbs), B4 and C2, that recognize post-ischemic neoepitopes following ischemia and reperfusion in other tissues. METHODS: Identification of post-SCI expressed neoepitopes was examined using previously characterized monoclonal Abs (B4 and C2 mAbs)...
June 19, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28627659/effects-of-saikosaponin-d-on-apoptosis-in-human-u87-glioblastoma-cells
#10
Yan Li, Tongjian Cai, Wei Zhang, Wenhe Zhu, Shijie Lv
Saikosaponin D (SSd) is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. SSd has been reported to exert anticancer activities. However, the effects of SSd on gliomas have not been elucidated. The aim of the present study was to investigate the pharmacological functions and potential molecular mechanisms of SSd in human U87 glioblastoma cells. The cells were treated with SSd at various concentrations for 48 h, the cell viability was assessed with 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium assay, and the activation of Akt, extracellular signal‑regulated kinases (ERK), c‑Jun N‑terminal kinases (JNK) and caspase‑3 was assessed by western blotting...
June 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627624/pro-oxidant-activity-of-sulforaphane-and-cisplatin-potentiates-apoptosis-and-simultaneously-promotes-autophagy-in-malignant-mesothelioma-cells
#11
Yoon-Jin Lee, Sang-Han Lee
Sulforaphane (SFN) is an isothiocyanate compound derived from glucoraphanin, which is found in cruciferous vegetables, and has been heralded as a chemopreventive and/or chemotherapeutic agent. The present study investigated the effects of SFN on enhancing the anticancer role of cisplatin (cis-dichlorodiammineplatinum; CDDP) in H‑28 malignant mesothelioma cells. At concentrations demonstrating limited toxicity in MeT‑5A normal human mesothelial cells, combination treatment with the two compounds exhibited synergistic growth‑inhibiting and apoptosis‑promoting activities, as demonstrated by a series of proapoptotic events, including reactive oxygen species accumulation, loss of mitochondrial membrane potential, upregulation of p53 expression, increased B‑cell lymphoma 2 (Bcl‑2) associated X protein/Bcl‑2 ratio, activation of caspase‑3, the occurrence of a sub‑G0/G1 peak and an increase in cells with pyknotic and fragmented nuclei, Annexin V‑phycoerythrin‑positive staining and G2/M phase‑transition delay in the cell cycle...
June 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627620/protective-effect-of-human-serum-amyloid-p-on-ccl4-induced-acute-liver-injury-in-mice
#12
Min Cong, Weihua Zhao, Tianhui Liu, Ping Wang, Xu Fan, Qingling Zhai, Xiaoli Bao, Dong Zhang, Hong You, Tatiana Kisseleva, David A Brenner, Jidong Jia, Hui Zhuang
Human serum amyloid P (hSAP), a member of the pentraxin family, inhibits the activation of fibrocytes in culture and inhibits experimental renal, lung, skin and cardiac fibrosis. As hepatic inflammation is one of the causes of liver fibrosis, in the present study, we investigated the hepatoprotective effects of hSAP against carbon tetrachloride (CCl4)-induced liver injury. Our data indicated that hSAP attenuated hepatic histopathological abnormalities and significantly decreased inflammatory cell infiltration and pro-inflammatory factor expression...
June 14, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28627461/8-methoxypsoralen-reduces-akt-phosphorylation-induces-intrinsic-and-extrinsic-apoptotic-pathways-and-suppresses-cell-growth-of-sk-n-as-neuroblastoma-and-sw620-metastatic-colon-cancer-cells
#13
Magdalena Bartnik, Adrianna Sławińska-Brych, Aleksandra Żurek, Martyna Kandefer-Szerszeń, Barbara Zdzisińska
ETHNOPHARMACOLOGICAL RELEVANCE: 8-methoxypsoralen (8-MOP) is a furanocoumarin and an active compound of a traditional Egyptian medicinal plant Ammi majus L, whose juice/fruit has been used for many years in folk phototherapy for the treatment of vitiligo or a hyperproliferative skin disorder, psoriasis. 8-MOP together with UVA light is also used as an anticancer drug for the treatment of cutaneous T-cell lymphoma. However, furanocoumarins exert anticancer activity even without UV irradiation...
June 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28627344/-protective-effect-of-bag-1l-mediated-by-lentivirus-in-human-neuroblastoma-cells-induced-by-hypoxia
#14
Yun Wang, Qingshu Li, Chao Jia, Chunyu Xie, Yankun Song, Nan Zhang, Yan Qu
OBJECTIVE: To investigate the protective effects of lentivirus mediated Bcl-2-associated athanogene 1L (BAG-1L) over-expression on human neuroblastoma cells (SH-SY5Y) induced by hypoxia/re-oxygenation, and to study its effect on the phosphoinositide 3 kinase serine/threonine protein kinase (PI3K/AKT) pathway. METHODS: SH-SY5Y cells were cultured in vitro, and the cells at logarithmic phase were collected, and they were divided into recombined lentiviral infection group [infected by lentivirus containing BAG-1L and green fluorescent protein (GFP) gene], vector control group (infected by lentivirus containing GFP without BAG-1L gene) and cell control group (non-infection)...
March 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28623712/deoxypodophyllotoxin-induces-cytoprotective-autophagy-against-apoptosis-via-inhibition-of-pi3k-akt-mtor-pathway-in-osteosarcoma-u2os-cells
#15
Sang-Hun Kim, Kyo-Min Son, Kwang-Youn Kim, Sun-Nyoung Yu, Sul-Gi Park, Young-Wook Kim, Hyo-Won Nam, Jeung-Tak Suh, Jae-Hoon Ji, Soon-Cheol Ahn
BACKGROUND: A natural compound deoxypodophyllotoxin (DPT) possesses potent anti-proliferative and anti-tumor properties on several cancer types. It triggers cell cycle arrest followed by apoptosis through various cellular processes. However, it is limited to the action mechanism of DPT-mediated cell death modes via apoptosis and autophagy. METHODS: Cell viability assay, morphological changes, annexin-V/propidium iodide (PI) assay, reactive oxygen species (ROS), acridine orange staining, and Western blot analyses were evaluated...
April 13, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28623398/endothelial-vascular-markers-in-coronary-surgery
#16
Diana M Valencia-Nuñez, Willy Kreutler, Javier Moya-Gonzalez, Pedro Alados-Arboledas, Ignacio Muñoz-Carvajal, Andrés Carmona, Rafael Ramirez-Chamond, Julia Carracedo-Añon
Coronary heart disease is associated with high morbidity and mortality. Endothelial dysfunction in affected patients is linked to long-term atherosclerotic disease progression and cardiovascular event rates. The present paper reports on changes in the levels of endothelial progenitor cells (VEGFR2/CD133/CD34), essential for endothelial repair, and of endothelial microvesicles (CD31/annexin V) as indicators of endothelial lesion, in patients undergoing coronary bypass surgery with respect both to baseline levels and to counts in healthy subjects...
June 16, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28623135/serum-long-non-coding-rna-malat-1-protected-by-exosomes-is-up-regulated-and-promotes-cell-proliferation-and-migration-in-non-small-cell-lung-cancer
#17
Rui Zhang, Yuhong Xia, Zhixin Wang, Jie Zheng, Yafei Chen, Xiaoli Li, Yu Wang, Huaikun Ming
Circulating lncRNAs have been defined as a novel biomarker for non-small cell lung cancer (NSCLC), MALAT-1 was first identified lncRNA that was related to lung cancer metastasis. However, the relationship between exosomal lncRNAs and the diagnosis and prognosis of NSCLC was poorly understood. The aim of this study is to evaluate the clinical significance of serum exosomal MALAT-1 as a biomarker in the metastasis of NSCLC. In this study, we firstly isolated the exosomes from healthy subjects and NSCLC patients...
June 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28622016/endoplasmic-reticulum-stress-is-involved-in-nucleus-pulposus-degeneration-and-attenuates-low-ph-induced-apoptosis-of-rat-nucleus-pulposus-cells
#18
Zhi-Yang Xie, Lu Chen, Feng Wang, Lei Liu, Cong Zhang, Kun Wang, Feng Cai, Arjun Sinkemanni, Xin Hong, Xiao-Tao Wu
The microenvironment of degenerative intervertebral disk (IVD) is characteristic of a high concentration of lactic acid and low pH levels, whereas the underlying mechanism has not been clearly defined. Endoplasmic reticulum (ER) is the hub of interactions between environmental signals and cellular biological functions, the malfunction of which is closely involved in the pathogenesis of multiple disorders, including IVD degeneration (IVDD). This research mainly aims at exploring what role ER stress plays in the natural process of IVDD and pH-induced apoptosis of rat nucleus pulposus (NP) cells (NPCs)...
June 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28621234/icarisid-ii-inhibits-the-proliferation-of-human-osteosarcoma-cells-by-inducing-apoptosis-and-cell-cycle-arrest
#19
Yuanyuan Tang, Mao Xie, Neng Jiang, Feifei Huang, Xiao Zhang, Ruishan Li, Jingjing Lu, Shijie Liao, Yun Liu
Icarisid II, one of the main active components of Herba Epimedii extracts, shows potent antitumor activity in various cancer cell lines, including osteosarcoma cells. However, the anticancer mechanism of icarisid II against osteosarcoma U2OS needs further exploration. This study aims to investigate further antitumor effects of icarisid II on human osteosarcoma cells and elucidate the underlying mechanism. We cultivated human osteosarcoma USO2 cells in vitro using different concentrations of icarisid II (0-30 µM)...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28620155/shp-2-activating-mutation-promotes-malignant-biological-behaviors-of-glioma-cells
#20
Yong Zhao, Lin Lin, Yonghui Zhang, Dangmurenjiafu Geng
BACKGROUND This study investigated the mechanism underlying the activating mutation of SHP-2 in promoting malignant biological behaviors of glioma cells. MATERIAL AND METHODS The SHP-2 empty plasmid pcDNA3.1 and SHP-2 activating mutation plasmid pcDNA3.1 SHP-2 D61G mutant eukaryotic expression vectors were designed; stable SHP-2-expressing cells transfected with pcDNA3.1 SHP-2 D61G mutant were set as the mutation group; cells transfected with pcDNA3.1 were set as the empty vector group; and cells without transfection were set as the control group...
June 16, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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