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https://www.readbyqxmd.com/read/29458150/pink1-protects-auditory-hair-cells-and-spiral-ganglion-neurons-from-cisplatin-induced-ototoxicity-via-inducing-autophagy-and-inhibiting-jnk-signaling-pathway
#1
Qianqian Yang, Gaoying Sun, Haiyan Yin, Hongrui Li, Zhixin Cao, Jinghan Wang, Meijuan Zhou, Haibo Wang, Jianfeng Li
Phosphatase and tensin homologue (PTEN)-induced putative kinase 1 (PINK1) gene encodes a serine/threonine kinase, which acts as a molecular sensor of mitochondrial health necessary for mitochondrial quality control. The present study was designed to examine whether PINK1 expressed in C57BL/6 murine cochlea and HEI-OC1 cells and, if so, to investigate the possible mechanisms underlying the action of PINK1 in cisplatin-induced death of sensory hair cells (HCs) and spiral ganglion neurons (SGNs) in vitro. The expression pattern of PINK1, formation of parkin particles, and autophagy were determined by immunofluorescent staining...
February 16, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29456703/silencing-of-tgif-sensitizes-mda-mb-231-human-breast-cancer-cells-to-cisplatin-induced-apoptosis
#2
Teng Pan, Tingting Mao, Haiyan Yang, Haiyu Wang, Yadong Wang
The present study was designed to explore the sensitivity of MDA-MB-231 cells to cisplatin after silencing the expression of TG-interacting factor (TGIF) protein. Cell viability was measured using an MTT assay. Cell apoptosis was detected by the annexin V and dead cell assay and the Hoechst staining assay. Protein expression was analyzed using western blot analysis. A colony formation assay was also performed. It was observed that cisplatin reduced the expression of TGIF protein in a dose- and time-dependent manner...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456109/synthetic-strigolactone-analogues-reveal-anti-cancer-activities-on-hepatocellular-carcinoma-cells
#3
Mohammed Nihal Hasan, Hani Choudhry, Syed Shoeb Razvi, Said Salama Moselhy, Taha Abduallah Kumosani, Mazin A Zamzami, Ziad Omran, Majed A Halwani, Salim Al-Babili, Khalid Omer Abualnaja, Abdulrahman Labeed Al-Malki, Mahmoud Alhosin, Tadao Asami
Hepatocellular carcinoma (HCC) remains one of the leading causes of death worldwide. The complex etiology is attributed to many factors like heredity, cirrhosis, hepatitis infections or the dysregulation of the different molecular pathways. Nevertheless, the current treatment regimens have either severe side effects or tumors gradually acquire resistance upon prolonged use. Thus, developing a new selective treatment for HCC is the need of the hour. Many anticancer agents derived from plants have been evaluated for their cytotoxicity towards many human cancer cell lines...
February 9, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29454770/molecular-imaging-of-apoptosis-in-ischemia-reperfusion-injury-with-radiolabeled-duramycin-targeting-phosphatidylethanolamine-effective-target-uptake-and-reduced-nontarget-organ-radiation-burden
#4
Hideki Kawai, Farhan Chaudhry, Aditya Shekhar, Artiom Petrov, Takehiro Nakahara, Takashi Tanimoto, Dongbin Kim, Jiqiu Chen, Djamel Lebeche, Francis G Blankenberg, Koon Y Pak, Frank D Kolodgie, Renu Virmani, Partho Sengupta, Navneet Narula, Roger J Hajjar, Harry W Strauss, Jagat Narula
OBJECTIVES: The purpose of this study was to evaluate the feasibility of imaging apoptosis in experimental ischemia-reperfusion model by technetium-99m (99m Tc)-labeled Duramycin, and compare it to an established tracer,99m Tc-labeled Annexin-V, which has a relative disadvantage of high radiation burden to nontarget organs. BACKGROUND: During apoptosis, the cell membrane phospholipids-phosphatidylserine (PS) and phosphatidylethanolamine (PE) are exposed and can be targeted by Annexin-V and Duramycin, respectively, for in vivo imaging...
February 9, 2018: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/29454291/de-novo-transcriptome-of-the-pallial-gland-of-the-date-mussel-lithophaga-lithophaga
#5
Urška Sivka, Nataša Toplak, Simon Koren, Jernej Jakše
Lithophaga lithophaga is a rock-boring bivalve with an ability to dissolve carbonate substrata with its siphonal and pallial gland secretions. However, the molecular mechanism that enables this species to bore into calcareous rocks is not yet known. In order to identify genes potentially involved in chemical boring we performed transcriptome sequencing of pallial-gland tissue samples of L. lithophaga. Transcriptome sequencing using an Ion Torrent platform generated 60.563 million clean reads with an average read length of 96 bp...
February 6, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29451308/apheresis-buffy-coat-collection-without-photoactivation-has-no-effect-on-apoptosis-cell-proliferation-and-total-viability-of-mononuclear-cells-collected-using-photopheresis-systems
#6
Zbigniew M Szczepiorkowski, Christine A Burnett, Larry J Dumont, Sunil H Abhyankar
BACKGROUND: Extracorporeal photopheresis (ECP) has been approved for the treatment of advanced cutaneous T-cell lymphoma since 1988. While the precise mechanisms resulting in clinical effects are not fully understood, the photoactivation of mononuclear cells (MNCs) using ultraviolet A (UVA) light and methoxsalen is believed to be the predominant initiating process. The effects of MNC passage through the instrument without photoactivation are unknown. The objective of this study was to evaluate the effect of cell processing through the photopheresis instruments on MNCs...
February 16, 2018: Transfusion
https://www.readbyqxmd.com/read/29449195/neutrophil-microvesicles-from-healthy-control-and-rheumatoid-arthritis-patients-prevent-the-inflammatory-activation-of-macrophages
#7
Hefin I Rhys, Francesco Dell'Accio, Costantino Pitzalis, Adrian Moore, Lucy V Norling, Mauro Perretti
Microvesicles (MVs) are emerging as a novel means to enact cell-to-cell communication in inflammation. Here, we aimed to ascertain the ability of neutrophil-derived MVs to modulate target cell behaviour, the focus being the macrophage. MVs were generated in response to tumour necrosis factor-α, from healthy control neutrophils or those from rheumatoid arthritis patients. MVs were used to stimulate human monocyte-derived macrophages in vitro, or administered intra-articularly in the K/BxN mouse model of arthritis...
February 7, 2018: EBioMedicine
https://www.readbyqxmd.com/read/29448507/platinum-nanoparticles-induce-apoptosis-on-raw-264-7-macrophage-cells
#8
Ta Thi Loan, Le Thanh Do, Hoon Yoo
The cellular effects of platinum nanoparticles (PNP05, average size of 5 nm, and PNP30, average size of 30 nm) were investigated on murine leukemia Raw 264.7 cells. Cells treated with various concentrations of PNPs showed size-dependent cytotoxicity in an MTT assay with PNP5 of smaller nanoparticles higher toxicity than PNP30. Investigations on cell morphology, Annexin V assay, DNA fragmentation and the activity of caspase-3/-7 showed that PNPs induced apoptosis on Raw 264.7 cells by changing cell morphology and density, increasing cell population in apoptosis and causing nucleus fragmentation...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448096/ampk-alleviates-endoplasmic-reticulum-stress-by-inducing-the-er-chaperone-orp150-via-foxo1-to-protect-human-bronchial-cells-from-apoptosis
#9
Ji-Qiang Liu, Li Zhang, Ji Yao, Shuo Yao, Ting Yuan
Chronic obstructive pulmonary disease (COPD), is characterized by inflammation of airways accompanied by a progressive destruction of lung parenchyma. This process is initiated in most cases by cigarette smoking. In this study we investigated the role of AMP activated protein kinase (AMPK) in cigarette smoke extract (CSE)-induced airway epithelial cell apoptosis as a consequence of endoplasmic reticulum stress (ER stress). Exposure of human bronchial epithelial cells (HBEpC) to CSE resulted in apoptosis as detected using Annexin V-PI flow cytometry...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29447541/jtc-801-exerts-anti-proliferative-effects-in-human-osteosarcoma-cells-by-inducing-apoptosis
#10
Chang-Jun Zheng, Li-Li Yang, Jun Liu, Lei Zhong
BACKGROUND: The research of G protein-coupled receptors (GPCRs) is a promising strategy for drug discovery. In cancer therapy, there is a need to discover novel agents that can inhibit proliferation and induce apoptosis in cancer cells. JTC-801 is a novel GPCR antagonist with the function of reversing pain and anxiety symptoms. This study aims to investigate the antitumor effects of JTC-801 on human osteosarcoma cells (U2OS) and elucidate the underlying mechanism. MATERIALS AND METHODS: The Cell Counting Kit-8 assay was used to detect the viability of U2OS cells treated with JTC-801 in vitro...
February 15, 2018: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/29445584/acid-sensing-ion-channel-1a-regulates-fate-of-rat-nucleus-pulposus-cells-in-acid-stimulus-through-endoplasmic-reticulum-stress
#11
Zhi-Yang Xie, Lu Chen, Cong Zhang, Lei Liu, Feng Wang, Feng Cai, Xiao-Hu Wang, Rui Shi, Arjun Sinkemani, Hao-Min Yu, Xin Hong, Xiao-Tao Wu
Acid-sensing ion channel 1a (ASIC1a) participates in human intervertebral disc degeneration (IVDD) and regulates the destiny of nucleus pulposus cells (NPCs) in acid stimulus. However, the mechanism of ASIC1a activation and its downstream pathway remain unclear. Endoplasmic reticulum (ER) stress also participates in the acid-induced apoptosis of NPCs. The main purpose of this study was to investigate whether there is any connection between ASIC1a and ER stress in an acid-induced nucleus pulposus degeneration model...
2018: BioResearch Open Access
https://www.readbyqxmd.com/read/29443732/curcumin-induces-apoptosis-and-inhibits-proliferation-in-infantile-hemangioma-endothelial-cells-via-downregulation-of-mcl-1-and-hif-1%C3%AE
#12
Suhui Lou, Yanfang Wang, Zujiang Yu, Kelei Guan, Quancheng Kan
BACKGROUND: Curcumin has been used as an alternative medicine for the treatment of infantile hemangiomas (IHs); however, the mechanism underlying the effectiveness of curcumin in IHs remains largely unclear. METHODS: In this study, we isolated primary human hemangioma endothelial cells (HemECs) from fresh surgical specimens of 3 patients. We treated HemECs by curcumin and investigated the alterations in proliferative and apoptotic signaling pathways with cell counting kit-8, flow cytometry, western blotting, immunofluorescence, and real-time polymerase chain reaction...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29442810/one-pot-facile-synthesis-of-pt-nanoparticles-using-cultural-filtrate-of-microgravity-simulated-grown-p-chrysogenum-and-their-activity-on-bacteria-and-cancer-cells
#13
Sivakumar Allur Subramaniyan, Sunirmal Sheet, Mohanraj Vinothkannan, Dong Jin Yoo, Yang Soo Lee, Shah Ahmed Belal, Kwan Seob Shim
Platinum nanoparticles (Pt NPs) was synthesized via a facile and cost competitive ont-pot green mediated synthesis using cell free cultural filtrate (microgravity simulated grown Penicillium chrysogenum) as a reducing agent. The toxicity effect of synthesized Pt NPs toward myoblast C2C12 carcinoma cells was then investigated. The particle size analyzer (DLS) and transmission electron microscopy (TEM) results demonstrates that both NG-Pt NPs and MG-Pt NPS are spherical in shape with an average diameter of 15 nm and 8...
May 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442262/nutrients-antioxidant-capacity-and-safety-of-hot-water-extract-from-sugar-maple-acer-saccharum-m-and-red-maple-acer-rubrum-l-bark
#14
Sagar Bhatta, Cristina Ratti, Patrice E Poubelle, Tatjana Stevanovic
Sugar maple (Acer saccharum M.) and red maple (Acer rubrum L.) barks were treated with hot water to extract nutrients in order to explore, for the first time, its potential as safe dietary antioxidants. The organic and inorganic nutrients of these extracts, as well as their safety on human PLB-985 cells differentiated into neutrophils-like cells, were determined. Proximate analysis showed that both bark extracts were low in moisture and fat. Sugar maple bark extract (SM-BX) showed crude protein and ash content higher than those found in red maple bark extract (RM-BX)...
February 13, 2018: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/29442021/hsp70-protects-human-trabecular-meshwork-cells-injury-induced-by-uvb-through-smad-pathway
#15
Yanna Cao, Ling Gao, Renhong Tang, Wenhua Zhang
AIM: Glaucoma is a universal eye disease which is commonly associated with increased intraocular pressure caused by impaired aqueous humor (AH) drainage. This study aimed to investigate the effects of Hsp70 on trabecular meshwork (TM) injury induced by UVB. METHODS: Real-time quantitative PCR (qRT-PCR) was used to examine the mRNA levels of Hsp70. siRNA was used to downregulate Hsp70 expression in the TM cells to inspect changes in cell proliferation and apoptosis...
June 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29442011/acetylpuerarin-protects-against-ogd-induced-cell-injury-in-bv2-microglia-by-inhibiting-hmgb1-release
#16
Deqing Sun, Aiying Xue, Xia Xue, Manru Ren, Jing Wu, Rongmei Wang
High mobility group box 1 (HMGB1), a non-histone DNA-binding protein, is massively released into the extracellular space from neuronal cells after ischemic injury, initiates inflammatory response and aggravates brain tissue damage. Acetylpuerarin (AP), an acetylated derivative of puerarin, was reported to protect against cerebrovascular ischemia-reperfusion injury in rats through anti-inflammation. In the present study, we aim to investigate whether AP inhibited HMGB1 release in oxygen-glucose deprivation (OGD)-treated BV2 microglia...
February 1, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29441993/klotho-protects-against-lps-induced-inflammation-injury-by-inhibiting-wnt-and-nf-%C3%AE%C2%BAb-pathways-in-hk-2-cells
#17
Yanmei Zhou, Yifang Kuang, Jianhua Zhou
AIM: This study aimed to investigate the effect and mechanism of Klotho in lipopolysaccharide (LPS)-induced inflammation injury in HK-2 cells. METHODS: We established LPS-induced inflammation injury model in HK-2 cells. The LPS-induced HK-2 cells were transfected with pc-Klotho, pcDNA3.1, siKlotho or siNC. Cell viability, apoptosis and reactive oxygen species (ROS) level were detected by MTT assay, Annexin V-FITC/PI Apoptosis Detection kit and 2,7-dichlorofluorescein diacetate, respectively...
April 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29440885/annexin-a1-is-elevated-in-patients-with-copd-and-affects-lung-fibroblast-function
#18
Tianwen Lai, Yanyu Li, Zongjiong Mai, Xiaoxia Wen, Yingying Lv, Zhanqing Xie, Quanchao Lv, Min Chen, Dong Wu, Bin Wu
Purpose: Fibrosis in peripheral airways is responsible for airflow limitation in chronic obstructive pulmonary disease (COPD). Annexin A1 modulates several key biological events during inflammation. However, little is known about its role in airway fibrosis in COPD. We investigated whether levels of Annexin A1 were upregulated in patients with COPD, and whether it promoted airway fibrosis. Methods: We quantified serum Annexin A1 levels in never-smokers (n=12), smokers without COPD (n=11), and smokers with COPD (n=22)...
2018: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29439254/inhibition-of-suicidal-erythrocyte-death-by-indirubin-3-monoxime
#19
Chunqiu Liu, Peipei Jiang, Yuanhong Xu, Meijuan Zheng, Jinpin Qiao, Xueyong Zhou, Dake Huang, Maohong Bian
BACKGROUND/AIMS: Qing Dai is a prized traditional Chinese medicine whose major component, indirubin, and its derivative, indirubin-3'-monoxime (IDM), have inhibitory effects on the growth of many human tumor cells and pronounced anti-leukemic activities. However, the effects of IDM on mature human erythrocytes are unclear. This study aimed to evaluate the potential impact of IDM on erythrocytes and the mechanisms underlying that impact. METHODS: Utilizing flow cytometry and confocal laser scanning microscopy, phosphatidylserine exposure at the cell surface was estimated by annexin V-fluorescein isothiocyanate (FITC)...
February 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29438580/drug-self-delivery-systems-based-on-hyperbranched-polyprodrugs-towards-tumor-therapy
#20
Jie Kong, Xiao Duan, Jianxin Chen, Yanlan Wu, Si Wu, Yan Shao
In this contribution, we developed amphiphilic hyperbranched polyprodrugs (DOX-S-S-PEG) with drug repeat units in hydrophobic core linked by disulfide bonds as drug self-delivery systems for cancer therapy. The amphiphilic micelles from DOX-S-S-PEG can be disrupted under glutathione (1mg mL-1) circumstance. Cell viability of A549 cells and 293T cells was evaluated by CCK-8 and Muse Annexin V & Dead Cell Kit. The disrupted polyprodrugs maintained drug activity for killing tumor cells. Meanwhile, the un-disrupted polyprodrugs possessed low cytotoxicity to normal cells...
February 13, 2018: Chemistry, An Asian Journal
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