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https://www.readbyqxmd.com/read/28110342/tgf-%C3%AE-1-smad3-signaling-pathway-suppresses-cell-apoptosis-in-cerebral-ischemic-stroke-rats
#1
Haiping Zhu, Qunfeng Gui, Xiaobo Hui, Xiaodong Wang, Jian Jiang, Lianshu Ding, Xiaoyang Sun, Yanping Wang, Huaqun Chen
BACKGROUND We desired to observe the changes of transforming growth factor-β1/drosophila mothers against decapentaplegic protein (TGF-β1/Smad3) signaling pathway in the hippocampus region of cerebral ischemic stroke rats so that the effects of this pathway on nerve cells can be investigated. MATERIAL AND METHODS The ischemic stroke models were built by middle cerebral artery occlusion (MCAO) in vivo and oxygen-glucose deprivation (OGD) in vitro. TGF-β1 and TGF-β1 inhibitors were injected into rat models while TGF-β1, TGF-β1 siRNA, Smad3, and Smad3 siRNA were transfected into cells...
January 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28110264/novel-phyto-derivative-brm270-inhibits-hepatocellular-carcinoma-cells-proliferation-by-inducing-g2-m-phase-cell-cycle-arrest-and-apoptosis-in-xenograft-mice-model
#2
Raj Kumar Mongre, Neelesh Sharma, Simrinder Singh Sodhi, Mrinmoy Ghosh, Amit Kumar Singh, Nameun Kim, Yang Ho Park, Young Gyu Shin, Sung Jin Kim, Zhang Jiao Jiao, Do Luong Huynh, Dong Kee Jeong
Hepatocellular carcinoma (HCC) is a major threat to human health worldwide and development of novel antineoplastic drug is demanding task. BRM270 is a proprietary combination of traditional medicinal herbs, has been shown to be effective against a wide range of stem-like cancer initiating cells (SLCICs). However, the underlying mechanism and antitumor efficacy of BRM270 in human hepatocellular carcinoma (HCC) cells have not been well elucidated till date. Here we studied the tumoricidal effect of BRM270 on human-CD133(+) expressing stem-like HepG-2 and SNU-398 cells...
January 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28110188/p53-dependent-apoptosis-and-cell-cycle-delay-induced-by-heteroleptic-complexes-in-human-cervical-cancer-cells
#3
Gunjan Sharma, Nishant Kumar Rana, Priya Singh, Pradeep Dubey, Daya Shankar Pandey, Biplob Koch
We previously reported synthesis of novel arene ruthenium (Ru) complexes and evaluated their antitumor activity in murine lymphoma (DL) cells. In this present study we further investigated the mechanism of action of two ruthenium complexes [complex 1 (η6-arene)RuCl(2-pcdpm)] and complex 2 (η6-arene)RuCl(4-mtdpm)] in cervical cancer cell line (HeLa). Our studies demonstrate that anticancer property of these two complexes was due to induction of apoptosis through p53 mediated pathway as well as arrest of cells in G2/M phase of cell cycle...
January 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28110119/interleukin-15-activates-akt-to-protect-astrocytes-from-oxygen-glucose-deprivation-induced-cell-death
#4
Gilbert Aaron Lee, Yein-Gei Lai, Ray-Jade Chen, Nan-Shih Liao
Astrocytes play a pivotal role in neuronal survival under the condition of post-ischemic brain inflammation, but the relevant astrocyte-derived mediators of ischemic brain injury remain to be defined. IL-15 supports survival of multiple lymphocyte lineages in the peripheral immune system, but the role of IL-15 in inflammatory disease of the central nervous system is not well defined. Recent research has shown an increase of IL-15-expressing astrocytes in the ischemic brain. Since astrocytes promote neuron survival under cerebral ischemia by buffering excess extracellular glutamate and producing growth factors, recovery of astrocyte function could be of benefit for stroke therapy...
January 18, 2017: Cytokine
https://www.readbyqxmd.com/read/28109698/microbubble-assisted-ultrasound-induced-transient-phosphatidylserine-translocation
#5
Jean-Michel Escoffre, Marc Derieppe, Bart Lammertink, Clemens Bos, Chrit Moonen
Microbubble-assisted ultrasound (sonopermeabilization) results in reversible permeabilization of the plasma membrane of cells. This method is increasingly used in vivo because of its potential to deliver therapeutic molecules with limited cell damage. Nevertheless, the effects of sonopermeabilization on the plasma membrane remain not fully understood. We investigated the influence of sonopermeabilization on the transverse mobility of phospholipids, especially on phosphatidylserine (PS) externalization. We performed studies using optical imaging with Annexin V and FM1-43 probes to monitor PS externalization of rat glioma C6 cells...
January 18, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28109008/inhibition-of-group-1-p21-activated-kinases-suppresses-pancreatic-stellate-cell-activation-and-increases-survival-of-mice-with-pancreatic-cancer
#6
Dannel Yeo, Phoebe Phillips, Graham S Baldwin, Hong He, Mehrdad Nikfarjam
Pancreatic cancer remains one of the most lethal of all solid tumours. Pancreatic stellate cells (PSCs) are primarily responsible for the fibrosis that constitutes the stroma and p21-activated kinase 1 (PAK1) may have a role in signalling pathways involving PSCs. This study aimed to examine the role of PAK1 in PSCs and in the interaction of PSCs with pancreatic cancer cells. Human PSCs were isolated using a modified outgrowth method. The effect of inhibiting PAK1 with a group 1 PAK inhibitor, FRAX597, on cell proliferation and apoptosis in vitro was measured by thymidine incorporation and annexin V assays, respectively...
January 21, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28108738/spect-and-pet-radiopharmaceuticals-for-molecular-imaging-of-apoptosis-from-bench-to-clinic
#7
Xiaobo Wang, Han Feng, Shichao Zhao, Junling Xu, Xinyu Wu, Jing Cui, Ying Zhang, Yuhua Qin, Zhiguo Liu, Tang Gao, Yongju Gao, Wenbin Zeng
Owing to the central role of apoptosis in many human diseases and the wide-spread application of apoptosis-based therapeutics, molecular imaging of apoptosis in clinical practice is of great interest for clinicians, and holds great promises. Based on the well-defined biochemical changes for apoptosis, a rich assortment of probes and approaches have been developed for molecular imaging of apoptosis with various imaging modalities. Among these imaging techniques, nuclear imaging (including single photon emission computed tomography and positron emission tomography) remains the premier clinical method owing to their high specificity and sensitivity...
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28108382/new-xanthones-and-cytotoxic-constituents-from-garcinia-mangostana-fruit-hulls-against-human-hepatocellular-breast-and-colorectal-cancer-cell-lines
#8
Gamal A Mohamed, Ahmed M Al-Abd, Ali M El-Halawany, Hossam M Abdallah, Sabrin R M Ibrahim
ETHNOPHARMACOLOGICAL RELEVANCE: Cancer has proceeded to surpass one of the most chronic illnesses to be the major cause of mortality in both the developing and developed world. Garcinia mangostana L. (mangosteen, family Guttiferae) known as the queen of fruits, is one of the most popular tropical fruits. It is cultivated in Southeast Asian countries: Malaysia, Indonesia, Sri Lanka, Burma, Thailand, and Philippines. Traditionally, numerous parts of G. mangostana have been utilized to treat various ailments such as abdominal pain, haemorrhoids, food allergies, arthritis, leucorrhoea, gonorrhea, diarrhea, dysentery, wound infection, suppuration, and chronic ulcer...
January 17, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28105340/antiproliferative-effect-of-me3738-a-derivative-of-soyasapogenol-on-hepatocellular-carcinoma-cell-lines-in-vitro-and-in-vivo
#9
Sachiko Ogasawara, Jun Akiba, Masamichi Nakayama, Hironori Kusano, Hirohisa Yano
Soyasapogenol, an aglycon of soyasaponin, ameliorates liver injury induced by concanavalin A in mice. A derivative of soyasapogenol, 22β-methoxyolean-12-ene-3β, 24(4β)-diol (ME3738), was reported to induce the gene expression of interferon (IFN)-β in hepatitis C virus replicon cells. The effect of ME3738 on hepatocellular carcinoma (HCC) cell lines was investigated in the present study. A total of 11 HCC cell lines were cultured in medium containing 0-10 µM ME3738, and after 24, 48, or 72 h of culture, morphological observation and MTT cell growth assays were performed...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28105237/xanthohumol-inhibits-proliferation-of-laryngeal-squamous-cell-carcinoma
#10
Yan Li, Kai Wang, Shankai Yin, Hongliang Zheng, Daliu Min
Xanthohumol is a flavonoid compound that exhibits antioxidant and anticancer effects, and is used to treat atherosclerosis. The aim of the present study was to investigate the effect of xanthohumol on the cell proliferation of laryngeal squamous cell carcinoma and to understand the mechanism of its action. The effects of xanthohumol on the cell viability and apoptosis rate of laryngeal squamous cell carcinoma SCC4 cells were assessed by Annexin V-fluorescein isothiocyanate/propidium iodide staining. In addition, the expression levels of pro-apoptotic proteins, caspase-3, caspase-8, caspase-9, poly ADP ribose polymerase (PARP) p53 and apoptosis-inducing factor (AIF), as well as anti-apoptotic markers, B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia 1 (Mcl-1), were analyzed by western blotting...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105222/curcumin-suppresses-the-proliferation-of-gastric-cancer-cells-by-downregulating-h19
#11
Gao Liu, Tian Xiang, Quan-Feng Wu, Wei-Xing Wang
Curcumin, a major phytochemical in turmeric, inhibits the proliferation of many types of solid cancer cells by enhancing p53 expression. However, the long non-coding RNA H19 directly inhibits p53 activation and thus promotes gastric cancer progression. The aim of this study was to assess the role of H19 in curcumin-induced proliferative inhibition of gastric cancer. The gastric cancer cell line SGC-7901 was treated with curcumin at different concentrations and time points. The effect of curcumin on proliferation was assessed using cell counting kit-8 assays and flow cytometry with Ki67 staining...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105121/expression-of-decorin-in-intestinal-tissues-of-mice-with-inflammatory-bowel-disease-and-its-correlation-with-autophagy
#12
Huazhou Zhao, Hongqing Xi, Bo Wei, Aizhen Cai, Ting Wang, Yi Wang, Xudong Zhao, Yanjing Song, Lin Chen
The aim of this study was to investigate the expression of decorin (DCN) in the intestinal tissues of mice with inflammatory bowel disease (IBD) and its correlation with autophagy. The IBD mouse model was created by intrarectal injection of trinitrobenzene sulfonic acid. The pathology of colon tissues of the mice was examined using hematoxylin and eosin staining. Expression of DCN and the proteins associated with autophagy was detected using immunohistochemistry. Normal human colon mucosal epithelial cells (NCM460 cells) were transfected with DCN expression plasmid and the expression of DCN and autophagy-associated proteins was detected by western blot analysis...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28105106/oxidized-low-density-lipoprotein-decreases-vegfr2-expression-in-huvecs-and-impairs-angiogenesis
#13
Min Zhang, Li Jiang
Atherosclerosis (AS), which is triggered by endothelial cell injury, evolves into a chronic inflammatory disease. Oxidized low-density lipoprotein (ox-LDL) is an important risk factor for the development of atherosclerosis; ox-LDL induces atherosclerotic plaque formation via scavenging receptors. The present study used ox-LDL-treated human umbilical vein endothelial cells (HUVECs) to investigate the effect of ox-LDL on angiogenesis. ox-LDL decreased HUVEC proliferation by MTT, induced apoptosis by Annexin V-fluorescein isothiocyanate (FITC) staining and markedly suppressed HUVEC tube formation by the Matrigel assay in a dose-dependent manner...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28105100/clinical-significance-of-leukocyte-associated-immunoglobulin-like-receptor-1-expression-in-human-cervical-cancer
#14
Yue Wang, Xueshan Zhang, Fang Miao, Yanning Cao, Jiangnan Xue, Qizhi Cao, Xiaoshu Zhang
Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is broadly expressed on the majority of immune cells; however, the biological role of LAIR in solid tumors has yet to be elucidated. In the present study, using immunohistochemical staining analysis, the expression of LAIR-1 in human cervical cancer (HCC) and nontumor-adjacent tissue specimens was determined, and the results indicated that the expression of LAIR-1 in HCC tissue was higher compared with that in noncancerous tissue. The χ(2) test was used to analyze the correlation between the expression of LAIR-1 in tumor tissues with clinicopathological parameters...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28104435/inhibition-of-nrf2-enhances-the-anticancer-effect-of-6-o-angeloylenolin-in-lung-adenocarcinoma
#15
Yang Wang, Ru-Yuan Yu, Jing Zhang, Wei-Xia Zhang, Zhi-Hao Huang, Hui-Fang Hu, Yao-Lan Li, Bin Li, Qing-Yu He
6-O-Angeloylenolin (6-OA), a sesquiterpene lactone isolated from Centipeda minima (L.) A. Br. (Compositae), has been used to treat respiratory diseases for centuries. However, whether and how 6-OA exerts anticancer effects against lung cancer remains to be elucidated. In this study, we showed that 6-OA markedly suppressed the cell viability and colony formation of lung cancer cells H1299 and A549, with no significant toxic effect on non-cancer cells HBE. Annexin V/7-AAD assay revealed that 6-OA induced cell apoptosis in dose- and time-dependent manners, which was further confirmed by the increased expression of cleaved caspase-3...
January 16, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28102740/update-on-functional-and-genetic-laboratory-assays-for-the-detection-of-platelet-microvesicles
#16
Maxime Laroche, Claire Dunois, Anne Marie Vissac, Jean Amiral
Functional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a thrombin generation assay (TGA) as "end points"; b) capture-based assays, in which PMVs bind to an immobilized ligand, such as Annexin V in the presence of calcium, or monoclonal antibodies (MoAbs) specific for membrane proteins...
January 19, 2017: Platelets
https://www.readbyqxmd.com/read/28101579/role-of-trna-selenocysteine-1-associated-protein-1-in-the-proliferation-and-apoptosis-of-cardiomyocyte%C3%A2-like-h9c2-cells
#17
Meng-Di Li, Wan-Peng Cheng, Min-Xia Shi, Tang-Dong Ge, Xiao-Lin Zheng, Dong-Yuan Wu, Xiao-Yan Hu, Jin-Cheng Luo, Feng-Lan Li, Hui Li
Transfer RNA selenocysteine 1 associated protein 1 (Trnau1ap) serves an essential role in the synthesis of selenoproteins, which have critical functions in numerous biological processes. Selenium deficiency results in a variety of diseases, including cardiac disease. However, the mechanisms underlying myocardial injury induced by selenium deficiency remain unclear. The present study examined the effects of Trnau1ap under‑ and overexpression in cardiomyocyte‑like H9c2 cells, by transfection with small interfering RNA and an overexpression plasmid, respectively...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28101240/downregulation-of-annexin-a1-is-correlated-with-radioresistance-in-nasopharyngeal-carcinoma
#18
Lifang Huang, Li Liao, Yanping Wan, Ailan Cheng, Meixiang Li, Sihan Chen, Maoyu Li, Xing Tan, Guqing Zeng
Radiotherapy is the primary treatment for nasopharyngeal carcinoma (NPC), but radioresistance often remains an obstacle to successful treatment. In our previous study, it was demonstrated that Annexin A1 (ANXA1) was involved in the p53-mediated radioresponse in NPC cells, which suggested that it may be associated with radioresistance in NPC; however, the role of ANXA1 in NPC radioresistance is unknown. In the present study, CNE2 cells were stably transfected with pLKO.1-ANXA1-small hairpin (sh)RNAs to investigate the effects of ANXA1 on the radiosensitivity of NPC...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101239/naphthazarin-suppresses-cell-proliferation-and-induces-apoptosis-in-human-colorectal-cancer-cells-via-the-b-cell-lymphoma-2-b-cell-associated-x-protein-signaling-pathway
#19
Ai-Dong Chen, Hui Li, Yong-Chun Li, Hai Zeng
Colorectal cancer is the most common gastrointestinal cancer in the USA. Naphthazarin, one of the naturally available 1,4-naphthoquinone derivatives, is a natural bioactive molecule that exhibits an antitumor effect. To the best of our knowledge, this is the first study to investigate the anticancer effect of naphthazarin on cell proliferation and apoptosis in human SW480 colorectal cancer cells. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to assess the effect of napthazarin on cell proliferation and cytotoxicity of SW430 cells, respectively...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101215/rottlerin-induced-autophagy-leads-to-apoptosis-in-bladder-cancer-cells
#20
Ping Qi, Zhenhua He, Lixiu Zhang, Yuan Fan, Zhiping Wang
It has been well-established that apoptosis contributes to cancer cell death; however, the role of autophagy in cancer cell death remains unclear. The aim of the present study was to investigate the effects of rottlerin, a traditional Indian medicine, on cell growth inhibition and autophagy in EJ human bladder carcinoma cells in vitro. Cell viability, measured by MTT assay, was found to be suppressed in a dose- and time-dependent manner. In addition, apoptosis was significantly increased in cells treated with rottlerin, as indicated by increased annexin V-fluorescein isothiocyanate/propidium iodide staining and changes in the cell cycle distribution that indicated blockage at G1 phase...
December 2016: Oncology Letters
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