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https://www.readbyqxmd.com/read/28549037/evaluation-of-a-dna-extraction-and-purification-protocol-using-archived-formalin-fixed-paraffin-embedded-tissues-for-braf-mutations-analysis-in-papillary-thyroid-microcarcinomas
#1
Adela Nechifor-Boilă, Andrada Loghin, Françoise Descotes, Myriam Decaussin-Petrucci, Angela Borda
The isolation of good quality genomic DNA from formalin-fixed, paraffin-embedded tissues is challenging, especially in cases of small tissue specimens. The aim of our study was to evaluate a DNA extraction protocol using formalin-fixed, paraffin-embedded tissues in our laboratory and apply this method to a series of papillary thyroid microcarcinomas (PTMCs). A total of 25 PTMCs and 3 papillary thyroid carcinoma control cases were included in the study. We assessed a DNA extraction protocol on the basis of a precipitation method (MasterPure DNA purification kit, Epicentre), according to the manufacturer's instructions...
May 25, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28548483/nanoscale-junction-formation-by-gas-phase-monolayer-doping
#2
Peyman Taheri, Hossain M Fahad, Mahmut Tosun, Mark Hettick, Daisuke Kiriya, Kevin Chen, Ali Javey
A major challenge in transistor technology scaling is the formation of controlled ultra-shallow junctions with nanometer-scale thickness and high spatial uniformity. Monolayer doping (MLD) is an efficient method to form such nanoscale junctions, where the self-limiting nature of semiconductor surfaces is utilized to form adsorbed monolayers of dopant-containing molecules followed by rapid thermal annealing (RTA) to diffuse the dopants to a desired depth. Unlike ion implantation, the process does not induce crystal damage, thus making it highly attractive for nanoscale transistor processing...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28546960/microshear-bond-strength-of-nano-bond-adhesive-containing-nanosized-aluminum-trioxide-particles
#3
Yousef Mohammed Althomali, Mohamed Ismail Ebrahim
OBJECTIVES: The present study was conducted to evaluate the effect of nanosized aluminum trioxide (Al2O3) particles when added to the Nano-Bond adhesive system and its effect on the microshear bond strength of nanocomposite resin to dentin. MATERIALS AND METHODS: A newly developed adhesive (Nano-Bond) and one type of light-cured resin restorative material (nanocomposite resin) were used in this study. The occlusal surfaces of extracted human molar teeth were ground perpendicular to the long axis of each tooth to expose a flat dentin surface...
April 2017: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/28544472/engineering-muscle-cell-alignment-through-3d-bioprinting
#4
Pamela Mozetic, Sara Maria Giannitelli, Manuele Gori, Marcella Trombetta, Alberto Rainer
Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell-seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment...
May 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28543961/automated-low-contrast-pattern-recognition-algorithm-for-magnetic-resonance-image-quality-assessment
#5
Morgan Ehman, Zhonghao Bao, Scott A Stiving, Mallik Kasam, Dianna Lanners, Teresa Peterson, Renee Jonsgaard, Rickey Carter, Kiaran P McGee
PURPOSE: Low contrast (LC) detectability is a common test criterion for diagnostic radiologic quality control (QC) programs. Automation of this test is desirable in order to reduce human variability and to speed up analysis. However, automation is challenging due to the complexity of the human visual perception system and the ability to create algorithms that mimic this response. This paper describes the development and testing of an automated LC detection algorithm for use in the analysis of magnetic resonance (MR) images of the American College of Radiology (ACR) QC phantom...
May 24, 2017: Medical Physics
https://www.readbyqxmd.com/read/28543702/microencapsulation-of-rifampicin-a-technique-to-preserve-the-mechanical-properties-of-bone-cement
#6
P Sanz-Ruiz, E Carbó-Laso, J C Del Real-Romero, F Arán-Ais, Y Ballesteros-Iglesias, E Paz-Jiménez, M Sánchez-Navarro, M A Pérez-Limiñana, J Vaquero-Martín
Two-stage exchange with antibiotic-loaded bone cement spacers remains the gold standard for chronic periprosthetic joint infection(PJI). Rifampicin is highly efficient on stationary-phase staphylococci in biofilm; however, its addition to PMMA to manufacture spacers prevents polymerization and reduces mechanical properties. Isolation of rifampicin during polymerization by microencapsulation could allow manufacturing rifampicin-loaded bone cement maintaining elution and mechanical properties. Microcapsules of rifampicin with alginate, polyhydroxybutyratehydroxyvalerate (PHBV), ethylcellulose and stearic acid (SA) were synthesized...
May 23, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28540709/rapd-scar-approaches-for-identification-of-adulterant-breeds-milk-in-dairy-products
#7
Joana T Cunha, Lucília Domingues
Food safety and quality are nowadays a major consumers' concern. In the dairy industry the fraudulent addition of cheaper/lower-quality milks from nonlegitimate species/breeds compromises the quality and value of the final product. Despite the already existing approaches for identification of the species origin of milk, there is little information regarding differentiation at an intra-species level. In this protocol we describe a low-cost, sensitive, fast, and reliable analytical technique-Random Amplified Polymorphic DNA/Sequence Characterized Amplified Region (RAPD/SCAR)-capable of an efficient detection of adulterant breeds in milk mixtures used for fraudulent manufacturing of dairy products and suitable for the detection of milk adulteration in processed dairy foods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28540708/fast-real-time-pcr-method-for-detection-of-soy-in-foods
#8
Montserrat Espiñeira, Francisco J Santaclara
Soy is used as an additive in the manufacturing of diverse products, because of their ability of emulsification, water and fat absorption, contributing to the consistency of food products. Moreover, soy is recognized as a potential allergen, so its presence should be indicated in all the food products.These issues highlight the need for techniques that allow the detection of soy in foods. This work describes a real-time PCR method for the detection of soy in a wide range of foodstuffs. The main features of this technique are its reliability and sensitivity, allowing the detection of trace amounts of soybean in processed products...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28540484/simultaneous-comparison-of-two-roller-compaction-techniques-and-two-particle-size-analysis-methods
#9
Tuomas Saarinen, Osmo Antikainen, Jouko Yliruusi
A new dry granulation technique, gas-assisted roller compaction (GARC), was compared with conventional roller compaction (CRC) by manufacturing 34 granulation batches. The process variables studied were roll pressure, roll speed, and sieve size of the conical mill. The main quality attributes measured were granule size and flow characteristics. Within granulations also the real applicability of two particle size analysis techniques, sieve analysis (SA) and fast imaging technique (Flashsizer, FS), was tested...
May 24, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28540299/surface-treatment-on-physical-properties-and-biocompatibility-of-orthodontic-power-chains
#10
H C Cheng, M S Chen, B Y Peng, W T Lin, Y K Shen, Y H Wang
The conventional orthodontic power chain, often composed of polymer materials, has drawbacks such as a reduction of elasticity owing to water absorption as well as surface discoloration and staining resulting from food or beverages consumed by the patient. The goal of this study was to develop a surface treatment (nanoimprinting) for orthodontic power chains and to alleviate their shortcomings. A concave template (anodic alumina) was manufactured by anodization process using pure aluminum substrate by employing the nanoimprinting process...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28535554/anesthetic-considerations-and-perioperative-management-of-spinal-cord-stimulators-literature-review-and-initial-recommendations
#11
Michael E Harned, Brandon Gish, Allison Zuelzer, Jay S Grider
BACKGROUND: Patients with implanted spinal cord stimulators (SCS) present to the anesthesia care team for management at many different points along the care continuum. Currently, the literature is sparse on the perioperative management. What is available is confusing; monopolar electrocautery is contraindicated but often used, full body magnetic resonance imaging (MRI) is safe with particular systems but with other manufactures only head and specific extremities exams are safe. Moreover, there are anesthetizing locations outside of the operating room where implanted SCS can interact with surrounding medical equipment and pose significant risk to patient and device...
May 2017: Pain Physician
https://www.readbyqxmd.com/read/28535038/fabrication-of-a-biodegradable-implant-with-tunable-characteristics-for-bone-implant-applications
#12
Iman Manavitehrani, Ali Fathi, Yiwei Wang, Peter K Maitz, Farid Mirmohseni, Tegan L Cheng, Lauren Peacock, David G Little, Aaron Schindeler, Fariba Dehghani
Biodegradable polymers are appealing material for the manufacturing of surgical implants as such implants break down in vivo, negating the need for a subsequent operation for removal. Many biocompatible polymers produce acidic breakdown products that can lead to localized inflammation and osteolysis. This study assesses the feasibility of fabricating implants out of poly(propylene carbonate) (PPC)-starch that degrades into CO2 and water. The basic compression modulus of PPC-starch (1:1 w/w) is 34 MPa; however, the addition of glycerol (1% w/w) and water as plasticizers doubles this value and enhances the surface wettability...
May 23, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28534832/the-boom-in-3d-printed-sensor-technology
#13
REVIEW
Yuanyuan Xu, Xiaoyue Wu, Xiao Guo, Bin Kong, Min Zhang, Xiang Qian, Shengli Mi, Wei Sun
Future sensing applications will include high-performance features, such as toxin detection, real-time monitoring of physiological events, advanced diagnostics, and connected feedback. However, such multi-functional sensors require advancements in sensitivity, specificity, and throughput with the simultaneous delivery of multiple detection in a short time. Recent advances in 3D printing and electronics have brought us closer to sensors with multiplex advantages, and additive manufacturing approaches offer a new scope for sensor fabrication...
May 19, 2017: Sensors
https://www.readbyqxmd.com/read/28534721/evaluation-of-3d-additively-manufactured-canine-brain-models-for-teaching-veterinary-neuroanatomy
#14
Regina M Schoenfeld-Tacher, Timothy J Horn, Tyler A Scheviak, Kenneth D Royal, Lola C Hudson
Physical specimens are essential to the teaching of veterinary anatomy. While fresh and fixed cadavers have long been the medium of choice, plastinated specimens have gained widespread acceptance as adjuncts to dissection materials. Even though the plastination process increases the durability of specimens, these are still derived from animal tissues and they require periodic replacement if used by students on a regular basis. This study investigated the use of three-dimensional additively manufactured (3D AM) models (colloquially referred to as 3D-printed models) of the canine brain as a replacement for plastinated or formalin-fixed brains...
May 23, 2017: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/28534676/applications-of-raman-spectroscopy-in-biopharmaceutical-manufacturing-a-short-review
#15
Kevin Buckley, Alan G Ryder
The production of active pharmaceutical ingredients (APIs) is currently undergoing its biggest transformation in a century. The changes are based on the rapid and dramatic introduction of protein- and macromolecule-based drugs (collectively known as biopharmaceuticals) and can be traced back to the huge investment in biomedical science (in particular in genomics and proteomics) that has been ongoing since the 1970s. Biopharmaceuticals (or biologics) are manufactured using biological-expression systems (such as mammalian, bacterial, insect cells, etc...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28534614/functional-nanoclay-suspension-for-printing-then-solidification-of-liquid-materials
#16
Yifei Jin, Ashley M Compaan, Wenxuan Chai, Yong Huang
Additive manufacturing (AM) enables the freeform fabrication of complex structures from various build materials. The objective of this study is to develop a novel Laponite nanoclay-enabled "printing-then-solidification" additive manufacturing approach to extrude complex three-dimensional (3D) structures made of various liquid build materials. Laponite, a member of the smectite mineral family, is investigated to serve as a yield-stress support bath material for the extrusion printing of liquid build materials...
May 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28532105/additive-manufacturing-with-polypropylene-microfibers
#17
Jodie N Haigh, Tim R Dargaville, Paul D Dalton
The additive manufacturing of small diameter polypropylene microfibers is described, achieved using a technique termed melt electrospinning writing. Sequential fiber layering, which is important for accurate three-dimensional fabrication, was achieved with the smallest fiber diameter of 16.4±0.2μm obtained. The collector speed, temperature and melt flow rate to the nozzle were optimized for quality and minimal fiber pulsing. Of particular importance to the success of this method is appropriate heating of the collector plate, so that the electrostatically drawn filament adheres during the direct-writing process...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532017/effects-of-pore-orientation-on-in-vitro-properties-of-retinoic-acid-loaded-plga-gelatin-scaffolds-for-artificial-peripheral-nerve-application
#18
Farnaz Ghorbani, Ali Zamanian, Hanieh Nojehdehian
Different manufacturing processes of scaffolds affect their main properties by altering the microstructure of pores. In this study, poly (lactic-co-glycolic acid) (PLGA) scaffolds were synthesized by both freeze casting and freeze drying methods. Scanning electron microscopy (SEM) micrographs demonstrated a unidirectional microstructure in the freeze-cast and a number of random pores in the freeze-dried scaffolds. According to the results, the formation of a lamellar microstructure increased the mechanical strength, swelling ratio, biodegradation behavior and drug release level...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531457/new-structural-insights-into-the-oligosaccharide-phosphate-fraction-of-pichia-hansenula-holstii-nrrl-y2448-phosphomannan
#19
Paul N Handley, Anthony Carroll, Vito Ferro
The oligosaccharide phosphate fraction (OPF) obtained from mild acid hydrolysis of P. holstii NRRL Y-2448 phosphomannan is the starting material for the preparation of the Phase III anticancer drug candidate PI-88. The OPF was for the first time successfully separated by preparative ion exchange chromatography and the major oligosaccharides isolated and characterized by NMR spectroscopy. The components were also acetylated and subjected to LC-MS analysis. These studies revealed that the OPF also contained all-α(1 → 3)-linked oligosaccharides in addition to the known α(1 → 3)/(1 → 2)-linked species, most likely formed by hydrolysis of the latter...
May 13, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28530715/nanofluidic-device-for-continuous-multiparameter-quality-assurance-of-biologics
#20
Sung Hee Ko, Divya Chandra, Wei Ouyang, Taehong Kwon, Pankaj Karande, Jongyoon Han
Process analytical technology (PAT) is critical for the manufacture of high-quality biologics as it enables continuous, real-time and on-line/at-line monitoring during biomanufacturing processes. The conventional analytical tools currently used have many restrictions to realizing the PAT of current and future biomanufacturing. Here we describe a nanofluidic device for the continuous monitoring of biologics' purity and bioactivity with high sensitivity, resolution and speed. Periodic and angled nanofilter arrays served as the molecular sieve structures to conduct a continuous size-based analysis of biologics...
May 22, 2017: Nature Nanotechnology
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