Praveen Weeratunga, Laura Denney, Joshua A Bull, Emmanouela Repapi, Martin Sergeant, Rachel Etherington, Chaitanya Vuppussetty, Gareth D H Turner, Colin Clelland, Jeongmin Woo, Amy Cross, Fadi Issa, Carlos Eduardo de Andrea, Ignacio Melero Bermejo, David Sims, Simon McGowan, Yasemin-Xiomara Zurke, David J Ahern, Eddie C Gamez, Justin Whalley, Duncan Richards, Paul Klenerman, Claudia Monaco, Irina A Udalova, Tao Dong, Agne Antanaviciute, Graham Ogg, Julian C Knight, Helen M Byrne, Stephen Taylor, Ling-Pei Ho
Single cell spatial interrogation of the immune-structural interactions in COVID -19 lungs is challenging, mainly because of the marked cellular infiltrate and architecturally distorted microstructure. To address this, we develop a suite of mathematical tools to search for statistically significant co-locations amongst immune and structural cells identified using 37-plex imaging mass cytometry. This unbiased method reveals a cellular map interleaved with an inflammatory network of immature neutrophils, cytotoxic CD8 T cells, megakaryocytes and monocytes co-located with regenerating alveolar progenitors and endothelium...
November 8, 2023: Nature Communications