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Zebrafish heart

Xiaojing Qiao, Yahui Liu, Peiqiang Li, Zhongzhong Chen, Huili Li, Xueyan Yang, Richard H Finnell, Zhangmin Yang, Ting Zhang, Bin Qiao, Yufang Zheng, Hongyan Wang
The planar cell polarity (PCP) pathway is critical for proper embryonic development of the neural tube and heart. Mutations in these genes have previously been implicated in the pathogenesis of neural tube defects (NTDs), but not in congenital heart defects (CHDs) in humans. We systematically identified the mutation patterns of CELSR1-3 , one family of the core PCP genes, in human cohorts composed of 352 NTD cases, 412 CHD cases, and matched controls. A total of 72 disease-specific rare novel coding mutations were identified, of which 37 were identified in CHD cases, and 36 were identified in NTD patients...
October 18, 2016: Clinical Science (1979-)
Akira Hanashima, Ken Hashimoto, Yoshihiro Ujihara, Takeshi Honda, Tomoko Yobimoto, Aya Kodama, Satoshi Mohri
Connectin, also called titin, is the largest protein with a critical function as a molecular spring during contraction and relaxation of striated muscle; its mutation leads to severe myopathy and cardiomyopathy. To uncover the cause of this pathogenesis, zebrafish have recently been used as disease models because they are easier to genetically modify than mice. Although the gene structures and putative primary structures of zebrafish connectin have been determined, the actual primary structures of zebrafish connectin in heart and skeletal muscles remain unclear because of its large size and the PCR amplification-associated difficulties...
October 7, 2016: Gene
Bomma Yashwanth, Rajesh Pamanji, J Venkateswara Rao
This study outlines the toxic effects of Quinalphos (QP), an organophosphrous insecticide on the development of zebrafish (Danio rerio) embryos, with special emphasis on toxicomorphomics and toxicokinetics of target enzyme, AChE. A range of concentrations was used to elucidate the median lethal concentration (LC50) of Quinalphos. Furthermore, embryos were exposed to two sub-lethal concentrations LC10 (0.66mg/L) and LC20 (1.12mg/L) along with a median lethal concentration (3.0mg/L) for 96h. Several morphological aberrations like lordosis, kyphosis, scoliosis, heart edema, breaks in the neuronal tube and underdeveloped facial parts were noticed, which were of concentration and time dependent...
October 1, 2016: Aquatic Toxicology
Gianfranco Matrone, John J Mullins, Carl S Tucker, Martin A Denvir
CDK9 is a known regulator of cellular transcription, growth and proliferation. Small molecule inhibitors are currently being developed and assessed in clinical trials as anti-cancer drugs. The zebrafish embryo provides an ideal model to explore the effects of CDK9 inhibition in-vivo. This has not been adequately explored previously at the level of a whole organism. We have compared and contrasted the effects of pharmacological and molecular inhibition of CDK9 on somatic growth, apoptosis and cellular proliferation in zebrafish larvae between 0 to 120 hours post fertilisation (hpf) using flavopiridol, a selective CDK9 antagonist, and CDK9-targeting morpholino...
October 7, 2016: Cell Cycle
Trina Manalo, Adam May, Joshua Quinn, Dominique S Lafontant, Olubusola Shifatu, Wei He, Juan M Gonzalez-Rosa, Geoffrey C Burns, Caroline E Burns, Alan R Burns, Pascal J Lafontant
Lectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish (Danio rerio) and giant danio (Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins-wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutinin (tomato lectin)-in these hearts...
September 28, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
Louis W Wang, Inken G Huttner, Celine F Santiago, Diane Fatkin
No abstract text is available yet for this article.
September 27, 2016: Zebrafish
Marta D' Amora, Marina Rodio, Juergen Bartelmess, Giuseppe Sancataldo, Rosaria Brescia, Francesca Cella Zanacchi, Alberto Diaspro, Silvia Giordani
Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging...
September 27, 2016: Scientific Reports
Rubén Marín-Juez, Michele Marass, Sebastien Gauvrit, Andrea Rossi, Shih-Lei Lai, Stefan C Materna, Brian L Black, Didier Y R Stainier
Zebrafish have a remarkable capacity to regenerate their heart. Efficient replenishment of lost tissues requires the activation of different cell types including the epicardium and endocardium. A complex set of processes is subsequently needed to support cardiomyocyte repopulation. Previous studies have identified important determinants of heart regeneration; however, to date, how revascularization of the damaged area happens remains unknown. Here, we show that angiogenic sprouting into the injured area starts as early as 15 h after injury...
October 4, 2016: Proceedings of the National Academy of Sciences of the United States of America
Liqin Yu, Yali Jia, Guanyong Su, Yongkai Sun, Robert J Letcher, John P Giesy, Hongxia Yu, Zhihua Han, Chunsheng Liu
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is a re-emerging environmental contaminant that has been frequently detected at sub-ppb (<μg/L) concentrations in natural waters. The objective of this study was to evaluate effects of TDCIPP on growth in initial generation (F0) zebrafish after chronic exposure to environmentally relevant concentrations, and to examine possible parental transfer of TDCIPP and transgenerational effects on growth of first generation (F1) larvae. When zebrafish (1-month old) were exposed to 580 or 7500 ng TDCIPP/L for 240 days, bioconcentration resulted in significantly less growth as measured by body length, body mass, brain-somatic index (BSI) and hepatic-somatic index (HSI) in F0 females but not F0 males...
September 16, 2016: Environmental Pollution
Yisong Zhen
BACKGROUND: Information on cardiovascular gene transcription is fragmented and far behind the present requirements of the systems biology field. To create a comprehensive source of data for cardiovascular gene regulation and to facilitate a deeper understanding of genomic data, the CardioTF database was constructed. The purpose of this database is to collate information on cardiovascular transcription factors (TFs), position weight matrices (PWMs), and enhancer sequences discovered using the ChIP-seq method...
2016: PeerJ
Feng Lv, Chenwen Zhu, Xinghong Yan, Xin Wang, Dong Liu
Transgenesis is an important tool for exploring gene expression and function. The myocyte enhancer factor 2a (mef2a) gene encodes a member of the Mef2 protein family that is involved in vertebrate skeletal, cardiac, and smooth muscle development and differentiation during myogenesis. According to studies on human and animal models, mef2a is highly expressed in the heart and somites. To explore the potential of mef2a as a tool for selective labeling of muscle cells in living zebrafish embryos, we constructed a transgene mef2aa:EGFP to induce the expression of green fluorescent protein (GFP) under the control of mef2a promoter...
September 8, 2016: Fish Physiology and Biochemistry
Susana S Lopes, Martin Distel, Claudia Linker, Rita Fior, Rui Monteiro, Isaac H Bianco, Ruben Portugues, Uwe Strähle, Leonor Saúde
The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Lisbon (Portugal) was voted by the European group leaders to be the setting for the 4th EZPM, and the organizing committee, composed by Leonor Saúde (iMM Lisboa, PT), Susana Lopes (CEDOC, PT), Michael Orger (Champalimaud Foundation, PT), Rui Oliveira (ISPA, PT), and António Jacinto (CEDOC, PT), was very enthusiastic to organize a productive event...
September 14, 2016: Zebrafish
Davor Lessel, Tariq Muhammad, Teresa Casar Tena, Barbara Moepps, Martin D Burkhalter, Marc-Phillip Hitz, Okan Toka, Axel Rentzsch, Stephan Schubert, Adelheid Schalinski, Ulrike M M Bauer, Christian Kubisch, Stephanie M Ware, Melanie Philipp
G protein-coupled receptor kinase 5 (GRK5) is a regulator of cardiac performance and a potential therapeutic target in heart failure in the adult. Additionally, we have previously classified GRK5 as a determinant of left-right asymmetry and proper heart development using zebrafish. We thus aimed to identify GRK5 variants of functional significance by analysing 187 individuals with laterality defects (heterotaxy) that were associated with a congenital heart defect (CHD). Using Sanger sequencing we identified two moderately frequent variants in GRK5 with minor allele frequencies <10%, and seven very rare polymorphisms with minor allele frequencies <1%, two of which are novel variants...
2016: Scientific Reports
Kar-Lai Poon, Michael Liebling, Igor Kondrychyn, Thomas Brand, Vladimir Korzh
The cardiac conduction system (CCS) propagates and coordinates the electrical excitation that originates from the pacemaker cells, throughout the heart, resulting in rhythmic heartbeat. Its defects result in life-threatening arrhythmias and sudden cardiac death. Understanding of the factors involved in the formation and function of the CCS remains incomplete. By transposon assisted transgenesis, we have developed enhancer trap (ET) lines of zebrafish that express fluorescent protein in the pacemaker cells at the sino-atrial node (SAN) and the atrio-ventricular region (AVR), termed CCS transgenics...
July 2016: Gene Expression Patterns: GEP
Molly Starkovich, Seema R Lalani, Catherine L Mercer, Daryl A Scott
Congenital heart defects (CHD) are present in over 1% of all newborns and are the leading cause of birth-defect-related deaths in the United States. We describe two male subjects with CHD, one with an atrial septal defect, a ventricular septal defect, and pulmonary artery stenosis; and the other with tetralogy of Fallot and a right aortic arch, who carry partially overlapping, de novo deletions of chromosome 5q33. The maximum region of overlap between these deletions encompasses HAND1 and SAP30L, two genes that have previously been shown to play a role in cardiac development...
September 2, 2016: American Journal of Medical Genetics. Part A
Feng Cheng, Liyun Miao, Qing Wu, Xia Gong, Jingwei Xiong, Jian Zhang
Coronary vessel development is a highly coordinated process during heart formation. Abnormal development and dysfunction of the coronary network are contributory factors in the majority of heart disease. Understanding the molecular mechanisms that regulate coronary vessel formation is crucial for preventing and treating the disease. We report a zebrafish gene-trap vinculin b (vclb) mutant that displays abnormal coronary vessel development among multiple cardiac defects. The mutant shows overproliferation of epicardium-derived cells and disorganization of coronary vessels, and they eventually die off at juvenile stages...
October 1, 2016: Development
M S W Xiang, K Kikuchi
Zebrafish possess a remarkable capacity for cardiac regeneration throughout their lifetime, providing a model for investigating endogenous cellular and molecular mechanisms regulating myocardial regeneration. By contrast, adult mammals have an extremely limited capacity for cardiac regeneration, contributing to mortality and morbidity from cardiac diseases such as myocardial infarction and heart failure. However, the viewpoint of the mammalian heart as a postmitotic organ was recently revised based on findings that the mammalian heart contains multiple undifferentiated cell types with cardiogenic potential as well as a robust regenerative capacity during a short period early in life...
2016: International Review of Cell and Molecular Biology
Yao Xiao, Maomao Gao, Luna Gao, Yu Zhao, Qiang Hong, Zhigang Li, Jing Yao, Hanhua Cheng, Rongjia Zhou
A cardiomyocyte differentiation in vitro system from zebrafish embryos remains to be established. Here, we have determined pluripotency window of zebrafish embryos by analyzing their gene-expression patterns of pluripotency factors together with markers of three germ layers, and have found that zebrafish undergoes a very narrow period of pluripotency maintenance from zygotic genome activation to a brief moment after oblong stage. Based on the pluripotency and a combination of appropriate conditions, we established a rapid and efficient method for cardiomyocyte generation in vitro from primary embryonic cells...
September 13, 2016: Stem Cell Reports
Hyun-Sik Nam, Kyu-Seok Hwang, Yun-Mi Jeong, Jeong-Im Ryu, Tae-Young Choi, Myung-Ae Bae, Woo-Chan Son, Kwan-Hee You, Hwa-Young Son, Cheol-Hee Kim
MicroRNA-122 (miRNA-122), also known as liver-specific miRNA, has recently been shown to be a potent biomarker in response to liver injury in mammals. The objective of this study was to examine its expression in response to toxicant treatment and acute liver damage, using the zebrafish system as an alternative model organism. For the hepatotoxicity assay, larval zebrafish were arrayed in 24-well plates. Adult zebrafish were also tested and arrayed in 200 mL cages. Animals were exposed to liver toxicants (tamoxifen or acetaminophen) at various doses, and miRNA-122 expression levels were analyzed using qRT-PCR in dissected liver, brain, heart, and intestine, separately...
2016: BioMed Research International
Swapnalee Sarmah, Pooja Muralidharan, James A Marrs
Fetal alcohol spectrum disorder (FASD), birth defects associated with ethanol exposure in utero, includes a wide spectrum of congenital heart defects (CHDs), the most prevalent of which are septal and conotruncal defects. Zebrafish FASD model was used to dissect the mechanisms underlying FASD-associated CHDs. Embryonic ethanol exposure (3-24 hours post fertilization) led to defects in atrio-ventricular (AV) valvulogenesis beginning around 37 hpf, a morphogenetic event that arises long after ethanol withdrawal...
2016: PloS One
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