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Zebrafish heart

Alysha D Cypher, Bryce Fetterman, Brian Bagatto
How fish respond to hypoxia, a common stressor, can be altered by simultaneous exposure to pollutants like bisphenol A (BPA), a plasticizer. BPA is cardiotoxic and interferes with the hypoxia inducible factor pathway (HIF-1α), therefore disrupting the hypoxic response. Co-exposure to hypoxia and BPA also causes severe bradycardia and reduced cardiac output in zebrafish larvae. The purpose of this work was to determine how the cardiovascular effects of co-exposure vary with BPA concentration and persist beyond exposure...
February 14, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
Esther Aix, Alex Gallinat, Ignacio Flores
Although recent advances have overturned the old view of the human heart as an inert postmitotic organ, it is clear that the adult heart´s capacity to regenerate after an ischemic episode is very limited. Unlike humans, zebrafish and other lower vertebrates vigorously regenerate damaged myocardium after cardiac injury. Understanding how the zebrafish is able to conserve life-long cardiac regeneration capacity while mammals lose it soon after birth is crucial for the development of new treatments for myocardial infarction...
February 3, 2018: Differentiation; Research in Biological Diversity
Maria A Missinato, Manush Saydmohammed, Daniel A Zuppo, Krithika S Rao, Graham W Opie, Bernhard Kühn, Michael Tsang
Zebrafish regenerate cardiac tissue through proliferation of pre-existing cardiomyocytes and neovascularization. Secreted growth factors such as FGFs, IGF, PDGFs, and Neuregulin (Nrg) play essential roles in stimulating cardiomyocyte proliferation. These factors activate the Ras/MAPK pathway, which is finely controlled by the feedback attenuator Dual Specificity Phosphatase 6 (Dusp6), an ERK phosphatase. Here we show that suppressing Dusp6 function enhanced cardiac regeneration. Inactivation of Dusp6 by small molecules or by gene inactivation increased cardiomyocyte proliferation, coronary angiogenesis, and reduced fibrosis after ventricular resection...
February 14, 2018: Development
Sellamani Muthulakshmi, Kannan Maharajan, Hamid R Habibi, Krishna Kadirvelu, Mudili Venkataramana
In the present study, we evaluated the zearalenone induced adverse effects in zebrafish embryos using various endpoints like embryo toxicity, heart rate, oxidative stress indicators (reactive oxygen species (ROS), lipid peroxidation (LPO), Nitric oxide (NO)), antioxidant responses (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase enzyme (GST) and reduced glutathione (GSH), metabolic biomarkers (lactate dehydrogenase (LDH) and Nitric oxide (NO)), neurotoxicity (acetylcholinesterase (AChE)), genotoxicity (comet assay and acridine orange staining (AO)) and histological analysis...
January 31, 2018: Chemosphere
Alfonso Saera-Vila, Ke'ale W Louie, Cuilee Sha, Ryan M Kelly, Phillip E Kish, Alon Kahana
Insulin-like growth factors (Igfs) are key regulators of key biological processes such as embryonic development, growth, and tissue repair and regeneration. The role of Igf in myogenesis is well documented and, in zebrafish, promotes fin and heart regeneration. However, the mechanism of action of Igf in muscle repair and regeneration is not well understood. Using adult zebrafish extraocular muscle (EOM) regeneration as an experimental model, we show that Igf1 receptor blockage using either chemical inhibitors (BMS754807 and NVP-AEW541) or translation-blocking morpholino oligonucleotides (MOs) reduced EOM regeneration...
2018: PloS One
Mark A Skarsfeldt, Sofia H Bomholtz, Pia R Lundegaard, Angelica Lopez-Izquierdo, Martin Tristani-Firouzi, Bo Hjorth Bentzen
AIM: The zebrafish has emerged as a novel model for investigating cardiac physiology and pathology. The aim of the current study is to investigate the atrium specific ion channels responsible for shaping the atrial cardiac action potential in zebrafish. METHODS: Using quantitative polymerase chain reaction we assessed the expression level of atrium specific potassium channels. The functional role of these channels was studied by patch clamp experiments on isolated atrial and ventricular cardiomyocytes, and by optical mapping of explanted adult zebrafish hearts...
February 7, 2018: Acta Physiologica
Lei Wang, Jae Young Oh, Hyun Soo Kim, WonWoo Lee, Yongri Cui, Hyo Geun Lee, Yong-Tae Kim, Jae Young Ko, You-Jin Jeon
Polysaccharides were extracted by Celluclast-assisted hydrolysis and ethanol precipitation from Hizikia fusiforme (HFPS), and their in vitro antioxidant effects were evaluated in Vero cells. HFPS contains 63.56 ± 0.32% sulfate polysaccharides, which comprise fucose (53.53%), galactose (23.15%), glucose (5.95%), and xylose (17.37%). HFPS scavenged DPPH, alkyl, and hydroxyl radicals at IC50 values of 0.81 ± 0.02, 0.25 ± 0.02, and 0.21 ± 0.03 mg/mL, respectively. HFPS significantly reduced H2O2-induced cytotoxicity in Vero cells...
February 1, 2018: International Journal of Biological Macromolecules
Yun Zhang, Saiyu Li, Juanjuan Li, Liwen Han, Qiuxia He, Rongchun Wang, Ximin Wang, Kechun Liu
The aims of this study were to investigate the mechanism underlying the developmental toxicity of fine particulate matter (PM2.5) and provide a more thorough understanding of the toxicity of PM2.5 in an ecological environment. Zebrafish embryos at 4 h post-fertilization were exposed to PM2.5 at doses of 200, 300, 400, 500, 600 and 800 μg/mL for 120 h. The mortality, hatching rate, morphology score, body length, locomotor capacity, histological changes, antioxidant defense system, leukocyte migration, inflammation-related gene mRNA expression, endoplasmic reticulum stress (ERS) and autophagy were evaluated to study PM2...
January 25, 2018: Chemosphere
Kannan Maharajan, Sellamani Muthulakshmi, Bojan Nataraj, Mathan Ramesh, Krishna Kadirvelu
Pyriproxyfen (2-[1-methyl-2-(4-phenoxyphenoxy) ethoxy] pyridine) (PPF), a pyridine-based pesticide widely used to control agricultural insect pests and mosquitoes in drinking water sources. However, its ecotoxicological data is limited in aquatic vertebrates particularly in fish. Hence, the present study aimed to evaluate the adverse effect of PPF in zebrafish embryo development (Danio rerio). In order to investigate the impact of PPF, embryos were exposed to 0.16, 0.33 and 1.66 μg/mL (0.52, 1.04 and 5.2 μM, respectively) for 96 hpf and various biomarker indices such as developmental toxicity (edema formation, hyperemia, heart size and scoliosis), oxidative stress (reactive oxygen species (ROS), lipid peroxidation (LPO) and nitric oxide (NO)), antioxidant responses (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx) and reduced glutathione (GSH)), biochemical (lactate dehydrogenase (LDH) and acid phosphatase (AP)), neurotoxicity (acetylcholinesterase (AChE)), genotoxicity (apoptosis and DNA damage) and histopathological changes were determined...
March 2018: Aquatic Toxicology
Chia-Ti Tsai, Ming-Wei Kuo, Jiunn-Lee Lin, Alice L Yu, John Yu
Heart failure is a major cardiovascular disease and is associated with significant morbidity and mortality. Sudden cardiac death (SCD) in heart failure is a disastrous cardiovascular phenomenon. However, few studies have examined the genetic background that determines susceptibility to heart failure and SCD. We found that deficiency of a newly identified gene, Yulink, promoted cardiac alternans in zebrafish cardiomyocytes, and genetic knockdown (KD) resulted in pericardial edema, decreased cardiac output, and premature ventricular contractions...
January 11, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Silja Barbara Burkhard, Jeroen Bakkers
Development of specialized cells and structures in the heart is regulated by spatially-restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, combining high-throughput RNA-sequencing with tissue-sectioning, to establish a genome-wide expression dataset with high spatial resolution for the developing zebrafish heart. Analysis of the dataset revealed over 1100 genes differentially expressed in sub-compartments...
February 5, 2018: ELife
Xiyan Mu, Ying Huang, Xuxing Li, Yunlei Lei, Miaomiao Teng, Xuefeng Li, Chengju Wang, Yingren Li
In order to understand the negative effects of bisphenol A (BPA) alternatives comprehensively, zebrafish embryos were used to assess the lethality, developmental effects, and estrogenic activity of bisphenol analogues. The in silico estrogenic activities of bisphenol analogues were assayed by binding simulation. According to our results, the lethality of bisphenol analogues decreased in order of bisphenol AF (BPAF) > BPA > bisphenol F (BPF) > bisphenol S (BPS). BPAF and BPF induced significant effects on zebrafish embryos, including decreased heart rate, hatching inhibition, and teratogenic effects...
February 14, 2018: Environmental Science & Technology
Héctor Sánchez-Iranzo, María Galardi-Castilla, Carolina Minguillón, Andrés Sanz-Morejón, Juan Manuel González-Rosa, Anastasia Felker, Alexander Ernst, Gabriela Guzmán-Martínez, Christian Mosimann, Nadia Mercader
During development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a+ cells, with a small contribution from tbx5a- SHF progenitors. Notably, ablation of ventricular tbx5a+-derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells...
January 30, 2018: Nature Communications
Danjun Ma, Chengjian Tu, Quanhu Sheng, Yuxi Yang, Zhisheng Kan, Yan Guo, Yu Shyr, Ian C Scott, Xin Lou
Failure to properly repair damaged due to myocardial infarction is a major cause of heart failure. In contrast to adult mammals, zebrafish hearts show remarkable regenerative capabilities after substantial damage. To characterize protein dynamics during heart regeneration, we employed an HPLC-ESI-MS/MS approach. Myocardium tissues were taken from sham operated fish and ventricle resected sample at three different time points (2 days, 7 days, and 14 days), dynamics of protein expression were analyzed by an ion current-based quantitative platform...
January 25, 2018: Journal of Proteome Research
Silja Burkhard, Vincent van Eif, Laurence Garric, Vincent M Christoffels, Jeroen Bakkers
The rhythmic contraction of the heart is initiated and controlled by an intrinsic pacemaker system. Cardiac contractions commence at very early embryonic stages and coordination remains crucial for survival. The underlying molecular mechanisms of pacemaker cell development and function are still not fully understood. Heart form and function show high evolutionary conservation. Even in simple contractile cardiac tubes in primitive invertebrates, cardiac function is controlled by intrinsic, autonomous pacemaker cells...
April 27, 2017: Journal of Cardiovascular Development and Disease
Laurent Gamba, Armaan Amin-Javaheri, Jieun Kim, David Warburton, Ching-Ling Lien
Myocardial infarction is the major cause of cardiac injury in western countries and can result in a massive loss of heart cells, leading eventually to heart failure. A fibrotic collagen-rich scar may prevent ventricular wall rupture, but also may result in heart failure because of its stiffness. In zebrafish, cardiac cryoinjury triggers a fibrotic response and scarring. Unlike with mammals, zebrafish heart has the striking ability to regenerate and to resolve the scar. Thus, understanding the mechanisms of scar resolution in zebrafish heart might facilitate the design of new therapeutic approaches to improve the recovery of patients...
February 24, 2017: Journal of Cardiovascular Development and Disease
Sophie Colombo, Carmen de Sena-Tomás, Vanessa George, Andreas A Werdich, Sunil Kapur, Calum A MacRae, Kimara L Targoff
NKX2-5 is the most commonly mutated gene associated with human congenital heart defects (CHDs) with a predilection for cardiac pole abnormalities. This homeodomain transcription factor is a central regulator of cardiac development and is expressed in both the first and second heart fields (FHF and SHF). We have previously revealed essential functions of nkx2.5 and nkx2.7, two Nkx2-5 homologues expressed in zebrafish cardiomyocytes, in maintaining ventricular identity. However, the differential roles of these genes in the specific subpopulations of the anterior (aSHF) and posterior (pSHF) SHFs have yet to be fully defined...
December 22, 2017: Development
Mengmeng Huang, Jingjing Jiao, Jun Wang, Zhidan Xia, Yu Zhang
Acrylamide (AA) is a high production volume chemical in industrial applications and widely found in baked or fried carbohydrate-rich foods. In this study, we unravelled that AA induced developmental toxicity associated with oxidative stress status and disordered lipid distribution in heart region of developing zebrafish. Treatment with AA caused a deficient cardiovascular system with significant heart malformation and dysfunction. We also found that AA could reduce the number of cardiomyocytes through the reduced capacity of cardiomyocyte proliferation rather than cell apoptosis...
January 8, 2018: Journal of Hazardous Materials
Niranjana Natarajan, Yamen Abbas, Donald M Bryant, Juan Manuel Gonzalez-Rosa, Michka Sharpe, Aysu Uygur, Lucas H Cocco-Delgado, Nhi Ngoc Ho, Norma P Gerard, Craig J Gerard, Calum A Macrae, Caroline E Burns, C Geoffrey Burns, Jessica L Whited, Richard T Lee
Background -Defining conserved molecular pathways in animal models of successful cardiac regeneration could yield insight into why adult mammals have inadequate cardiac regeneration after injury. Insight into the transcriptomic landscape of early cardiac regeneration from model organisms will shed light on evolutionarily conserved pathways in successful cardiac regeneration. Methods -Here we describe a cross-species transcriptomic screen in three model organisms for cardiac regeneration -axolotl, neonatal mice and zebrafish...
January 18, 2018: Circulation
Navina Panneerselvan, Malathi Ragunathan
Hypoxia is known to be a major player during pathological angiogenesis and adenosine as a negative feedback signaling to maintain oxygen delivery in pathological ischemic condition. We mimicked hypoxic condition and studied angiogenesis by inducing adenosine receptors using forskolin, a plant compound and NECA analogue of adenosine using zebrafish model. Vascular endothelial growth factor (VEGF) is known to play a key role during pathological angiogenesis and regulated by the factors HIF1a under hypoxic condition and recently Notch is proposed to play a negative feedback loop mechanism along with VEGF signaling but the role of adenosine receptor during the process is not known...
January 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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