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https://www.readbyqxmd.com/read/28216038/de-novo-transcriptome-of-the-muga-silkworm-antheraea-assamensis-helfer
#1
Hasnahana Chetia, Debajyoti Kabiraj, Deepika Singh, Ponnala Vimal Mosahari, Suradip Das, Pragya Sharma, Kartik Neog, Swagata Sharma, P Jayaprakash, Utpal Bora
Antheraea assamensis (Lepidoptera: Saturniidae), is a semi-domesticated silkworm known to be endemic to Assam and the adjoining hilly areas of Northeast India. It is the only producer of a unique, commercially important variety of golden silk called "muga silk". Herein, we report the de novo transcriptome of A. assamensis reared on Machilus bombycina leaves for the first time. Short reads generated by high throughput sequencing of cDNA libraries from multiple tissues, viz. alimentary canal, silk gland and residual body were assembled into transcripts via a de novo assembly pipeline followed by functional annotation and classification...
February 16, 2017: Gene
https://www.readbyqxmd.com/read/28204597/cohort-profile-the-taiwan-mj-cohort-half-a-million-chinese-with-repeated-health-surveillance-data
#2
Xifeng Wu, Shan Pou Tsai, Chwen Keng Tsao, Mu Lin Chiu, Min Kuang Tsai, Po Jung Lu, June Han Lee, Chien Hwa Chen, Christopher Wen, Shu-Sen Chang, Chung Y Hsu, Chi Pang Wen
No abstract text is available yet for this article.
February 15, 2017: International Journal of Epidemiology
https://www.readbyqxmd.com/read/28186172/appropriateness-of-reference-genes-for-normalizing-messenger-rna-in-mouse-2-4-dinitrobenzene-sulfonic-acid-dnbs-induced-colitis-using-quantitative-real-time-pcr
#3
Nour Eissa, Laëtitia Kermarrec, Hayam Hussein, Charles N Bernstein, Jean-Eric Ghia
2,4-Dinitrobenzene sulfonic acid (DNBS)-induced colitis is an experimental model that mimics Crohn's disease. Appropriateness of reference genes is crucial for RT-qPCR. This is the first study to determine the stability of reference gene expression (RGE) in mice treated with DNBS. DNBS experimental Colitis was induced in male C57BL/6 mice. RNA was extracted from colon tissue and comprehensive analysis of 13 RGE was performed according to predefined criteria. Relative colonic TNF-α and IL-1β mRNA levels were calculated...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28166455/structural-and-mechanistic-insights-into-nuclear-transport-and-delivery-of-the-critical-pluripotency-factor-oct4-to-dna
#4
Takahide Okuyama, Ryosuke Yamagishi, Jiro Shimada, Masaaki Ikeda, Yayoi Maruoka, Hiroki Kaneko
Oct4 is a master regulator of the induction and maintenance of cellular pluripotency, and has crucial roles in early stages of differentiation. It is the only factor that cannot be substituted by other members of the same protein family to induce pluripotency. However, although Oct4 nuclear transport and delivery to target DNA are critical events for reprogramming to pluripotency, little is known about the molecular mechanism. Oct4 is imported to the nucleus by the classical nuclear transport mechanism, which requires importin α as an adaptor to bind the nuclear localization signal (NLS)...
February 6, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28164233/the-use-of-quinacrine-in-nitroimidazole-resistant-giardia-duodenalis-an-old-drug-for-an-emerging-problem
#5
Ana Requena-Méndez, Pilar Goñi, Encarnación Rubio, Diana Pou, Victoria Fumadó, Silvia Lóbez, Edelweiss Aldasoro, Juan Cabezos, Maria Eugenia Valls, Begoña Treviño, Antonio Federico Martínez Montseny, Antonio Clavel, Joaquim Gascon, José Muñoz
No abstract text is available yet for this article.
February 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28146557/costs-of-transmission-assessment-surveys-to-provide-evidence-for-the-elimination-of-lymphatic-filariasis
#6
Molly A Brady, Rachel Stelmach, Margaret Davide-Smith, Jim Johnson, Bolivar Pou, Joseph Koroma, Kingsley Frimpong, Angela Weaver
BACKGROUND: To reach the global goal of elimination of lymphatic filariasis as a public health problem by 2020, national programs will have to implement a series of transmission assessment surveys (TAS) to determine prevalence of the disease by evaluation unit. It is expected that 4,671 surveys will be required by 2020. Planning in advance for the costs associated with these surveys is essential to ensure that the required resources are available for this essential program activity. METHODOLOGY AND FINDINGS: Retrospective cost data was collected from reports from 13 countries which implemented a total of 105 TAS surveys following a standardized World Health Organization (WHO) protocol between 2012 and 2014...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28124980/evolutionary-conservation-of-pou5f3-genomic-organization-and-its-dynamic-distribution-during-embryogenesis-and-in-adult-gonads-in-japanese-flounder-paralichthys-olivaceus
#7
Jinning Gao, Xubo Wang, Quanqi Zhang
Octamer-binding transcription factor 4 (Oct4) is a member of POU (Pit-Oct-Unc) transcription factor family Class V that plays a crucial role in maintaining the pluripotency and self-renewal of stem cells. Though it has been deeply investigated in mammals, its lower vertebrate homologue, especially in the marine fish, is poorly studied. In this study, we isolated the full-length sequence of Paralichthys olivaceus pou5f3 (Popou5f3), and we found that it is homologous to mammalian Oct4. We identified two transcript variants with different lengths of 3'-untranslated regions (UTRs) generated by alternative polyadenylation (APA)...
January 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28123573/transcription-factor-oct4-promotes-osteosarcoma-by-regulating-lncrna-ak055347
#8
Hongwu Fan, Guangyao Liu, Changfu Zhao, Xuefeng Li, Xiaoyu Yang
Osteosarcoma is the most common primary bone tumor in children and adolescents, typically presenting with a poor prognosis. Octamer-binding transcription factor 4 (Oct4) protein, encoded by the POU class 5 homeobox 1 gene, is important in maintaining self-renewal of pluripotent stem cells, and is closely associated with cancer. However, its role in osteosarcoma remains to be elucidated. The present study observed Oct4 was markedly increased in osteosarcoma cell lines and in human osteosarcoma tissue samples...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/28100850/molecular-characteristic-and-physiological-role-of-dopa-decarboxylase
#9
Joanna Guenter, Robert Lenartowski
The enzyme DOPA decarboxylase (aromatic-L-amino-acid decarboxylase, DDC) plays an important role in the dopaminergic system and participates in the uptake and decarboxylation of amine precursors in the peripheral tissues. Apart from catecholamines, DDC catalyses the biosynthesis of serotonin and trace amines. It has been shown that the DDC amino acid sequence is highly evolutionarily conserved across many species. The activity of holoenzyme is regulated by stimulation/blockade of membrane receptors, phosphorylation of serine residues, and DDC interaction with regulatory proteins...
December 31, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28087091/effect-of-single-dose-of-tolvaptan-in-neurocritical-patients-with-hyponatremia-due-to-syndrome-of-inappropriate-antidiuretic-hormone-secretion
#10
J A Llompart-Pou, J Pérez-Bárcena, M Novo, J M Raurich
No abstract text is available yet for this article.
January 10, 2017: Medicina Intensiva
https://www.readbyqxmd.com/read/28073447/potential-impact-of-an-exceptional-bloom-of-karenia-mikimotoi-on-dissolved-oxygen-levels-in-waters-off-western-ireland
#11
Shane O'Boyle, Georgina McDermott, Joe Silke, Caroline Cusack
In the summer of 2005 an exceptional bloom of the dinoflagellate Karenia mikimotoi occurred along Ireland's Atlantic seaboard and was associated with the mass mortality of both benthic and pelagic marine life. Oxygen depletion, cellular toxicity and physical smothering, are considered to be the main factors involved in mortality. In this paper we use a theoretical approach based on stoichiometry (the Anderson ratio) and an average K. mikimotoi cellular carbon content of 329pgCcell(-1) (n=20) to calculate the carbonaceous and nitrogenous oxygen demand following bloom collapse...
March 2016: Harmful Algae
https://www.readbyqxmd.com/read/28051029/a-pou3f4-mutation-causes-nonsyndromic-hearing-loss-in-a-chinese-x-linked-recessive-family
#12
Wan Du, Ming-Kun Han, Da-Yong Wang, Bing Han, Liang Zong, Lan Lan, Ju Yang, Qi Shen, Lin-Yi Xie, Lan Yu, Jing Guan, Qiu-Ju Wang
BACKGROUND: The molecular genetic research showed the association between X-linked hearing loss and mutations in POU3F4. This research aimed to identify a POU3F4 mutation in a nonsyndromic X-linked recessive hearing loss family. METHODS: A series of clinical evaluations including medical history, otologic examinations, family history, audiologic testing, and a high-resolution computed tomography scan were performed for each patient. Bidirectional sequencing was carried out for all polymerase chain reaction products of the samples...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28044321/combined-effect-of-virus-infection-and-water-stress-on-water-flow-and-water-economy-in-grapevines
#13
Hanan El Aou-Ouad, Alicia Pou, Magdalena Tomàs, Rafael Montero, Miquel Ribas-Carbo, Hipólito Medrano, Josefina Bota
Water limitation is one of the major threats affecting grapevine production. Thus, improving water use efficiency (WUE) is crucial for a sustainable viticulture industry in Mediterranean regions. Under field conditions, water stress is often combined with viral infections since those are present in major grape-growing areas worldwide. Grapevine leafroll-associated virus 3 (GLRaV-3) is one of the most important viruses, affecting grapevines. Indeed, the optimization of water use in a real context of virus infection is an important topic that needs to be understood...
January 3, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28013048/pou1f1-the-key-transcription-factor-related-to-somatic-growth-in-tilapia-orechromis-niloticus-is-regulated-by-two-independent-post-transcriptional-regulation-mechanisms
#14
Dongfang Wang, Jingkai Qin, Jirong Jia, Peipei Yan, Wensheng Li
This study aims to determine the post-transcriptional regulation mechanism of the transcription factor pou1f1 (pou class 1 homeobox 1), which is the key gene for pituitary development, somatic growth in vertebrates, and transcription of several hormone genes in teleost fish. MicroRNA miR-223-3p was identified as a bona fide target of pou1f; overexpression of miR-223-3p in primary pituitary cells led to the down-regulation of pou1f1 and downstream genes, and inhibition of miR-223-3p led to the up-regulation of pou1f1 in Nile tilapia dispersed primary pituitary cells...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28007765/changing-pou-dimerization-preferences-converts-oct6-into-a-pluripotency-inducer
#15
Stepan Jerabek, Calista Kl Ng, Guangming Wu, Marcos J Arauzo-Bravo, Kee-Pyo Kim, Daniel Esch, Vikas Malik, Yanpu Chen, Sergiy Velychko, Caitlin M MacCarthy, Xiaoxiao Yang, Vlad Cojocaru, Hans R Schöler, Ralf Jauch
The transcription factor Oct4 is a core component of molecular cocktails inducing pluripotent stem cells (iPSCs), while other members of the POU family cannot replace Oct4 with comparable efficiency. Rather, group III POU factors such as Oct6 induce neural lineages. Here, we sought to identify molecular features determining the differential DNA-binding and reprogramming activity of Oct4 and Oct6. In enhancers of pluripotency genes, Oct4 cooperates with Sox2 on heterodimeric SoxOct elements. By re-analyzing ChIP-Seq data and performing dimerization assays, we found that Oct6 homodimerizes on palindromic OctOct more cooperatively and more stably than Oct4...
February 2017: EMBO Reports
https://www.readbyqxmd.com/read/28006058/an-expansile-mass-of-the-sphenoid-sinus-or-petrous-apex
#16
Jason D Pou, Michael J Marino, Rizwan Aslam
No abstract text is available yet for this article.
December 22, 2016: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/27986639/identification-of-the-binding-domains-and-key-amino-acids-for-the-interaction-of-the-transcription-factors-bmpoum2-and-bmabd-a-in-bombyx-mori
#17
Yong He, Huimin Deng, Qihao Hu, Zidan Zhu, Lin Liu, Sichun Zheng, Qisheng Song, Qili Feng
The transcription factor BmPOUM2 interacted with another transcription factor BmAbd-A to regulate the expression of the wing cuticle protein gene BmWCP4 in Bombyx mori. In this study, the binding domains and amino acids for the interaction between BmPOUM2 and BmAbd-A were reported. Two isoforms of BmPOUM2 were identified. The short isoform (BmPOUM2-S) lacks a 114-amino acid sequence containing a POU-homeodomain and a nuclear localization signal peptide (NLS), as compared to the full-length isoform (BmPOUM2)...
February 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27941302/effects-of-downregulating-tead4-transcripts-by-rna-interference-on-early-development-of-bovine-embryos
#18
Nobuyuki Sakurai, Kazuki Takahashi, Natsuko Emura, Tsutomu Hashizume, Ken Sawai
Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages...
December 11, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27930301/reprogramming-cell-fate-with-a-genome-scale-library-of-artificial-transcription-factors
#19
Asuka Eguchi, Matthew J Wleklinski, Mackenzie C Spurgat, Evan A Heiderscheit, Anna S Kropornicka, Catherine K Vu, Devesh Bhimsaria, Scott A Swanson, Ron Stewart, Parameswaran Ramanathan, Timothy J Kamp, Igor Slukvin, James A Thomson, James R Dutton, Aseem Z Ansari
Artificial transcription factors (ATFs) are precision-tailored molecules designed to bind DNA and regulate transcription in a preprogrammed manner. Libraries of ATFs enable the high-throughput screening of gene networks that trigger cell fate decisions or phenotypic changes. We developed a genome-scale library of ATFs that display an engineered interaction domain (ID) to enable cooperative assembly and synergistic gene expression at targeted sites. We used this ATF library to screen for key regulators of the pluripotency network and discovered three combinations of ATFs capable of inducing pluripotency without exogenous expression of Oct4 (POU domain, class 5, TF 1)...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27929731/autophagic-homeostasis-is-required-for-the-pluripotency-of-cancer-stem-cells
#20
Tanveer Sharif, Emma Martell, Cathleen Dai, Barry E Kennedy, Patrick Murphy, Derek R Clements, Youra Kim, Patrick W K Lee, Shashi A Gujar
Pluripotency is an important feature of cancer stem cells (CSCs) that contributes to self-renewal and chemoresistance. The maintenance of pluripotency of CSCs under various pathophysiological conditions requires a complex interaction between various cellular pathways including those involved in homeostasis and energy metabolism. However, the exact mechanisms that maintain the CSC pluripotency remain poorly understood. In this report, using both human and murine models of CSCs, we demonstrate that basal levels of autophagy are required to maintain the pluripotency of CSCs, and that this process is differentially regulated by the rate-limiting enzyme in the NAD(+) synthesis pathway NAMPT (nicotinamide phosphoribosyltransferase) and the transcription factor POU5F1/OCT4 (POU class 5 homeobox 1)...
February 2017: Autophagy
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