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Andrea Bowe, Susanne Zweerink, Vera Mück, Vangelis Kondylis, Sigrid Schulte, Tobias Goeser, Dirk Nierhoff
There is a medical need of biomarkers for disease stratification in cholestatic liver diseases that come along with changes in hepatocyte polarity. Neighbor of Punc E11 (Nope) is an oncofetal marker that is lost after final differentiation and polarization of hepatocytes. We analyzed the expression pattern of Nope and connexin (Cx) 26 as markers of hepatocyte polarization during murine liver development as well as in adult liver with or without bile duct ligation (BDL) by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), western blotting (WB), and immunohistochemistry...
April 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
A Padilla-Galo, C Olveira, L Fernández de Rota-Garcia, I Marco-Galve, A J Plata, A Alvarez, F Rivas-Ruiz, A Carmona-Olveira, J J Cebrian-Gallardo, M A Martinez-Garcia
BACKGROUND: Some studies have reported a high prevalence of bronchiectasis in patients with uncontrolled asthma, but the factors associated with this condition are unknown. The objective of this study was to determine the prevalence of bronchiectasis in uncontrolled moderate-to-severe asthma and to identify risk factors and their correlation with bronchiectasis in these patients. METHODS: This is a prospective study of data from consecutive patients with uncontrolled moderate-to-severe asthma...
March 16, 2018: Respiratory Research
Dmitriy A Shagin, Maria A Turchaninova, Irina A Shagina, Mikhail Shugay, Andrew R Zaretsky, Olga I Zueva, Dmitriy A Bolotin, Sergey Lukyanov, Dmitriy M Chudakov
BACKGROUND: Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3'-end of unwanted amplification primer (NOPE oligo) simultaneously provides a template for its elongation. This elongation disrupts specificity of unwanted primer, preventing its further participation in PCR. The suggested approach allows to rationally manage the course of PCR reactions in order to facilitate analysis of complex DNA mixtures as well as to perform multistage PCR bypassing intermediate purification steps...
June 5, 2017: BMC Genomics
Gregory Fischer, Fei Wang, Hongfei Xiang, Xiaowen Bai, Hongwei Yu, Quinn H Hogan
Injury-induced neuropathic pain remains a serious clinical problem. Recent studies indicate that bone marrow stromal cells (BMSCs) effectively attenuate chronic neuropathic pain in animal models. Here, we examined the therapeutic effect of intrathecal administration of BMSCs isolated from young (1-month-old) rats on pain hypersensitivity induced by tibial nerve injury. Cerebrospinal fluid (CSF) was collected and analyzed to examine the effect of BMSC administration on the expression of 67 soluble factors in CSF...
September 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
David C Sheridan, Kyle P Johnson, Rongwei Fu, David M Spiro, Matthew L Hansen
OBJECTIVES: Mental health complaints are a frequent presentation to the pediatric emergency department (PED). It is unclear if having an on-site inpatient pediatric psychiatric unit impacts pediatric mental health care in the acute setting. The objective of this study was to compare PED mental health care between a pediatric tertiary care center with an associated inpatient child psychiatric unit (PAPED) and one that does not (NOPED) with the hypothesis that children have longer lengths of stay (LOS) at the PED without an inpatient unit...
January 2017: Pediatric Emergency Care
Bao Men, Yanzhi Sun, Mujie Li, Chaoqun Hu, Man Zhang, Linan Wang, Yang Tang, Yongmei Chen, Pingyu Wan, Junqing Pan
Hierarchical nitrogen-doped porous graphene/carbon (NPGC) composites were fabricated by a simple and nontemplate method. The morphology characterizations demonstrate that reduced graphene oxide was successfully coated by the carbon derived from glucose, and a well-organized and interpenetrated hierarchical porous structure of NPGC was formed after pyrolysis at 950 °C. Notably, the prepared material, denoted as NPGC-950, has superlarge specific surface area (1510.83 m(2) g(-1)) and relatively high content percentage of pyridinic and graphitic nitrogen...
January 20, 2016: ACS Applied Materials & Interfaces
Yogen Singh, Samir Gupta, Alan M Groves, Anjum Gandhi, John Thomson, Shakeel Qureshi, John M Simpson
UNLABELLED: Targeted echocardiographic assessments of haemodynamic status are increasingly utilised in many settings. Application in the neonatal intensive care units (NICU) is increasingly demanded but challenging given the risk of underlying structural lesions. This statement follows discussions in UK led by the Neonatologists with an Interest in Cardiology and Haemodynamics (NICHe) group in collaboration with the British Congenital Cardiac Association (BCCA) and the Paediatricians with Expertise in Cardiology Special Interest Group (PECSIG)...
February 2016: European Journal of Pediatrics
Luc Mertens
No abstract text is available yet for this article.
February 2016: European Journal of Pediatrics
Young Hee Kim, Kyung A Kwak, Jin Seok Kang
To identify preneoplastic markers for hepatocarcino-genesis, the expression levels of neighbor of Punc E11 (Nope), E-cadherin and α-fetoprotein (AFP) were investigated in carcinogen-treated embryonic cell lineages. Mouse embryonic stem cells (ESCs), hepatic progenitor cells (HPCs), and hepatocyte‑like cells (HCs) representing 0, 22, and 40 days of liver differentiation, respectively, were treated in vitro with diethylnitrosamine (DEN) at 4 doses (0, 1, 5, and 15 mM) for 24 h. The expression of Nope, E-cadherin and AFP was examined by quantitative real-time PCR, western blotting and immunocytochemistry...
September 2014: Oncology Reports
Claudio W Pikielny
Hydrocarbon pheromones on the cuticle of Drosophila melanogaster modulate the complex courtship behavior of males. Recently, three members of the degenerin/epithelial Na+ channel (DEG/ENaC) family of sodium channel subunits, Ppk25, Ppk23, and Ppk29 (also known as Nope), have been shown to function in gustatory perception of courtship-modulating contact pheromones. All three proteins are required for the activation of male courtship by female pheromones. Specific interactions between two of them have been demonstrated in cultured cells, suggesting that, in a subset of cells where they are coexpressed, these three subunits function within a common heterotrimeric DEG/ENaC channel...
2012: Science Signaling
Gerald T Mangine, Adam M Gonzalez, Adam J Wells, William P McCormack, Maren S Fragala, Jeffrey R Stout, Jay R Hoffman
BACKGROUND: A dietary supplement containing a blend of 170 mg of N-oleyl-phosphatidylethanolamine (NOPE) and 100 mg of epigallocatechin-3-gallate (EGCG) has been shown to improve compliance to low caloric diets. Considering the cost of dietary ingredients, many manufacturers attempt to determine the lowest efficacious dose. Thus, the purpose of this study was to evaluate the efficacy of 8-weeks of supplementation with a daily intake of 120 mg of NOPE and 105 mg of EGCG in conjunction with a low caloric diet and regular, moderate exercise on dietary compliance in healthy, overweight adults...
2012: Lipids in Health and Disease
Tong Liu, Elena Starostina, Vinoy Vijayan, Claudio W Pikielny
Trimeric sodium channels of the DEG/ENaC family have important roles in neurons, but the specific functions of different subunits present in heteromeric channels are poorly understood. We previously reported that the Drosophila DEG/ENaC subunit Ppk25 is essential in a small subset of gustatory neurons for activation of male courtship behavior, likely through detection of female pheromones. Here we show that, like mutations in ppk25, mutations in another Drosophila DEG/ENaC subunit gene, nope, specifically impair male courtship of females...
August 22, 2012: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Stephanie Schievenbusch, Elisabeth Sauer, Harald-Morten Curth, Sigrid Schulte, Münevver Demir, Ulrich Toex, Tobias Goeser, Dirk Nierhoff
We have previously identified Neighbor of Punc E 11 (Nope) as a specific cell surface marker of stem/progenitor cells in the murine fetal liver that is also expressed in hepatocellular carcinoma. Here, we focus on the differential expression pattern of Nope during murine fetal and postnatal liver development as well as in a normal and regenerating adult liver including oval cell activation. In the fetal liver, Nope shows a constantly high expression level and is a useful surface marker for the identification of Dlk, E-cadherin, and CD133-positive hepatoblasts by flow cytometry...
September 20, 2012: Stem Cells and Development
L Ciotta, M Stracquadanio, C Formuso, S Di Leo, A Andò, I Pagano
Adjustment and maintenance of body weight are the result of many process combination, that affect both the gastrointestinal system and other mechanisms in the central nervous system. Often a diet modification alone is not sufficient to guarantee significant changes in body weight. For this reason, it sometimes necessary to make other interventions, in order to help an individual to adhere to the diet as much as possible and to achieve the objectives established. The N-oleyl-phosphatidyl-ethanolamine (NOPE) is a phospholipid...
September 2011: Minerva Gastroenterologica e Dietologica
Luz M Ballesteros, Santiago Martín, Gorka Pera, Phil A Schauer, Nicola J Kay, María Carmen López, Paul J Low, Richard J Nichols, Pilar Cea
Langmuir films have been fabricated from 4-[4'-(4''-thioacetyl-phenyleneethynylene)-phenyleneethynylene]-aniline (NOPES) after cleavage of the thioacetyl protecting group. Characterization by surface pressure vs area per molecule isotherms and Brewster angle microscopy reveal the formation of a high quality monolayer at the air-water interface. One layer Langmuir-Blodgett (LB) films were readily fabricated by the transfer of the NOPES Langmuir film onto solid substrates. X-ray photoelectron spectroscopy (XPS), surface polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS), and quartz crystal microbalance (QCM) experiments conclusively demonstrate the formation of one layer LB films in which the functional group associated with binding to the substrate can be tailored by the film transfer conditions...
April 5, 2011: Langmuir: the ACS Journal of Surfaces and Colloids
Keiko F Takahashi, Tamotsu Kiyoshima, Ieyoshi Kobayashi, Ming Xie, Haruyoshi Yamaza, Hiroaki Fujiwara, Yukiko Ookuma, Kengo Nagata, Hiroko Wada, Takako Sakai, Yoshihiro Terada, Hidetaka Sakai
BACKGROUND: Protogenin (Prtg) has been identified as a gene which is highly expressed in the mouse mandible at embryonic day 10.5 (E10.5) by a cDNA subtraction method between mandibles at E10.5 and E12.0. Prtg is a new member of the deleted in colorectal carcinoma (DCC) family, which is composed of DCC, Neogenin, Punc and Nope. Although these members play an important role in the development of the embryonic central nervous system, recent research has also shed on the non-neuronal organization...
2010: BMC Developmental Biology
Jens Uwe Marquardt, Maria Quasdorff, Heike Varnholt, Harald-Morten Curth, Senait Mesghenna, Ulrike Protzer, Tobias Goeser, Dirk Nierhoff
Hepatocellular carcinoma (HCC) is the 5th common malignancy worldwide, but established markers fail to detect up to one third of HCC. We have recently identified Neighbor of Punc E11 (Nope) as a surface marker for murine fetal liver stem cells. Similar to commonly used HCC markers such as α-Fetoprotein (Afp) and Glypican-3 (Gpc-3), we here establish Nope as an oncofetal marker of murine and human HCC and investigate its specific expression in hepatoma cell lines and primary HCC. Murine and human hepatoma cell lines and Cre-inducible SV40 T-antigen transgenic mice (Alb-SV40TAg(ind) ) were analyzed for Nope expression in comparison to common HCC markers by quantitative RT-PCR, Western blot analyses and immunohistochemistry...
May 15, 2011: International Journal of Cancer. Journal International du Cancer
P McCulloch, A Mishra, A Handa, T Dale, G Hirst, K Catchpole
Unintended harm to patients in operating theatres is common. Correlations have been demonstrated between teamwork skills and error rates in theatres. This was a single-institution uncontrolled before-after study of the effects of "non-technical" skills training on attitudes, teamwork, technical performance and clinical outcome in laparoscopic cholecystectomy (LC) and carotid endarterectomy (CEA) operations. The setting was the theatre suite of a UK teaching hospital. Attitudes were measured using the Safety Attitudes Questionnaire (SAQ)...
April 2009: Quality & Safety in Health Care
Mariangela Rondanelli, Annalisa Opizzi, Sebastiano Bruno Solerte, Rosita Trotti, Catherine Klersy, Roberta Cazzola
Many studies have found that N-oleyl-ethanolamine (NOE), a metabolite of N-oleyl-phosphatidylethanolamine (NOPE), and epigallocatechin-3-gallate (EGCG) inhibit food intake. The main aim of this study was to evaluate the efficacy of 2 months of administration of an oily NOPE-EGCG complex (85 mg NOPE and 50 mg EGCG per capsule) and its effect on compliance with diet in healthy, overweight people. Secondary end-points of the study were to compare body composition, metabolic parameters, sensation of appetite, depressive symptoms and severity of binge eating...
February 2009: British Journal of Nutrition
Yue Zheng, Xiao-Rui Cheng, Wen-Xia Zhou, Yong-Xiang Zhang
Alzheimer's disease (AD) is a progressive, neurodegenerative disease, which primarily affects the elderly. Clinical signs of AD are characterized by the neuron loss and cognitive impairment. At gene and protein levels, the senescence-accelerated mouse/prone 8 (SAMP8) is a suitable animal model to investigate the fundamental mechanisms of age-related learning and memory deficits. Huang-Lian-Jie-Du decoction (HL), a well-known traditional Chinese medicinal prescription, has been employed in the treatment of wide range of disease conditions...
July 11, 2008: Neuroscience Letters
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