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https://www.readbyqxmd.com/read/29776613/high-resolution-melting-analysis-hrm-for-the-assessment-of-clonality-in-feline-b-cell-lymphomas
#1
Manfred Henrich, Svenja Scheffold, Werner Hecht, Manfred Reinacher
Analysis of clonality is gaining importance in diagnosing lymphomas in veterinary medicine. Usually, PCR for the analysis of antigen receptor rearrangement (PARR) is followed by electrophoretic separation of the PCR products. Aim of this study was to test the feasibility of HRM for the assessment of clonality in B-cell lymphomas of cats. High resolution melting analysis differentiates PCR products by their different melting point using the decrease in fluorescence of an intercalating dye during melting of the PCR product...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29776457/a-new-green-method-for-the-quantitative-analysis-of-enrofloxacin-by-fourier-transform-infrared-spectroscopy
#2
Camila Tavares Rebouças, Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Background: A green analytical chemistry method was developed for quantification of enrofloxacin in tablets. The drug, a second-generation fluoroquinolone, was first introduced in veterinary medicine for the treatment of various bacterial species. Objective: This study proposed to develop, validate, and apply a reliable, low-cost, fast, and simple IR spectroscopy method for quantitative routine determination of enrofloxacin in tablets. Methods: The method was completely validated according to the International Conference on Harmonisation guidelines, showing accuracy, precision, selectivity, robustness, and linearity...
May 18, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/29775839/veterinary-applications-of-pulsed-electromagnetic-field-therapy
#3
REVIEW
James S Gaynor, Sean Hagberg, Blake T Gurfein
Pulsed electromagnetic field (PEMF) therapy can non-invasively treat a variety of pathologies by delivering electric and magnetic fields to tissues via inductive coils. The electromagnetic fields generated by these devices have been found to affect a variety of biological processes and basic science understanding of the underlying mechanisms of action of PEMF treatment has accelerated in the last 10 years. Accumulating clinical evidence supports the use of PEMF therapy in both animals and humans for specific clinical indications including bone healing, wound healing, osteoarthritis and inflammation, and treatment of post-operative pain and edema...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29770227/severe-oesophageal-constriction-due-to-an-aberrant-right-subclavian-artery-with-kommerell-s-diverticulum-in-a-cat
#4
Ryohei Suzuki, Yohei Mochizuki, Hiroki Yoshimatsu, Ayaka Niina, Takahiro Teshima, Hirotaka Matsumoto, Hidekazu Koyama
Case summary: A 6-month-old female cat with a history of chronic regurgitation presented with weight loss. CT angiography (CTA) showed severe constriction of the oesophagus due to an aberrant right subclavian artery and Kommerell's diverticulum. The cat was surgically treated and remained clinically normal. More than 1 year after surgery, the general condition of the cat was stable and body weight had increased. Relevance and novel information: This case report describes the clinical findings and surgical management of a cat diagnosed with severe oesophageal constriction caused by an aberrant right subclavian artery with Kommerell's diverticulum (markedly dilated origin of the aberrant right subclavian artery), which are rare vascular anomalies in veterinary medicine and specifically in cats...
January 2018: JFMS Open Reports
https://www.readbyqxmd.com/read/29770051/agriculture-and-agri-food-canada-s-research-program-on-antimicrobial-resistance
#5
E Topp
A key strategy for attenuating the development of antimicrobial resistance (AMR) is ensuring judicious use of antimicrobials in human and veterinary medicine and in agriculture. Research on AMR in agriculture includes risk assessment, risk management, and identifying the role of agricultural practices in development of AMR. Risk assessment includes an impact assessment of antimicrobial use in livestock and on the environment; for example, many antimicrobials are excreted unchanged and thus reach the environment through manure application...
November 2, 2017: Canada Communicable Disease Report, Relevé des Maladies Transmissibles Au Canada
https://www.readbyqxmd.com/read/29770049/pan-canadian-framework-for-action-on-antimicrobial-resistance-and-antimicrobial-use
#6
(no author information available yet)
Antimicrobial-resistant infections are becoming more frequent and increasingly difficult to treat, and this situation is exacerbated by the widespread use of antimicrobials in both human and veterinary medicine and by the agriculture industry. As part of Canada's coordinated response to addressing antimicrobial resistance (AMR), Tackling Antimicrobial Resistance and Antimicrobial Use: A Pan-Canadian Framework for Action, was released in September 2017. The Framework is a high-level policy document that outlines the strategic objectives, outcomes and opportunities to guide collaborative action on AMR and antimicrobial use (AMU)...
November 2, 2017: Canada Communicable Disease Report, Relevé des Maladies Transmissibles Au Canada
https://www.readbyqxmd.com/read/29769009/mesenchymal-stem-cell-research-in-veterinary-medicine
#7
Mudasir Bashir Gugjoo, Amarpal, Vikash Chandra, Mohd Yaqoob Wani, Kuldeep Dhama, Gutulla Taru Sharma
BACKGROUND: The stem cells due to their unique characteristic features like self-renewal, multiplication, immuno-modulation and differentiation, are seen and hoped to bring new horizons in the field of biology, in general and medicine, in particular. OBJECTIVE: Among various stem cell types, currently mesenchymal stem cells (MSCs) are extensively studied in regenerative medicine owing to their readily available sources, easy harvesting and ability to differentiate both into mesodermal as well as non-mesodermal tissues with little associated ethical issues...
May 16, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29767571/student-perceptions-of-veterinary-anatomy-practical-classes-a-longitudinal-study
#8
Erica Gummery, Kate A Cobb, Liz H Mossop, Malcolm A Cobb
Using cadaveric material to teach veterinary students poses many challenges. However, little research exists on the contribution of this traditional approach to student learning. This longitudinal study aimed to investigate student perceptions of cadaver-based anatomy classes in a vertically integrated veterinary curriculum at the University of Nottingham's School of Veterinary Medicine and Science. Likert-scale statements and free-text boxes were used in a questionnaire distributed to second-year veterinary students (response rate 59%, 61/103)...
2018: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/29767568/the-relationship-between-intuitive-action-choices-and-moral-reasoning-on-animal-ethics-issues-in-students-of-veterinary-medicine-and-other-relevant-professions
#9
Joy M Verrinder, Clive J C Phillips
With growing understanding of animals' capabilities, and public and organizational pressures to improve animal welfare, moral action by veterinarians and other relevant professionals to address animal issues is increasingly important. Little is known about how their action choices relate to their moral reasoning on animal ethics issues. A moral judgment measure, the VetDIT, with three animal and three non-animal scenarios, was used to investigate the action choices of 619 students in five animal- and two non-animal-related professional programs in one Australian university, and how these related to their moral reasoning based on Personal Interest (PI), Maintaining Norms (MN), or Universal Principles (UP) schemas...
2018: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/29767451/feline-ureteral-obstructions-part-1-medical-management
#10
REVIEW
D L Clarke
Feline ureteral obstructions are an increasingly recognised and challenging diagnostic and management problem. Many cats with ureteral obstructions are critically ill at the time of diagnosis, especially if there is dysfunction of the contralateral kidney. They may present with varying severities of acute kidney injury, electrolyte disturbances, and may have comorbidities such as heart disease that complicate perioperative and long-term management. Medical management, which may consist of rehydration and restoration of intravascular volume with intravenous fluid therapy, osmotic diuresis, ureteral muscle relaxation, and antimicrobials for infection, is important in feline ureteral obstruction patients...
May 16, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29765971/alopecia-areata-in-a-dog-clinical-dermoscopic-and-histological-features
#11
Fabia Scarampella, Paola Roccabianca
Alopecia areata (AA)-like disease is characterized by multifocal patchy hair loss in humans, rodents, dogs, and horses. Remarkable similarities between human and nonhuman AA cases have been reported in terms of clinical presentation, histology, and immune mechanisms of the disease. Canine AA-like lesions most often consist of well-demarcated alopecic patches, frequently but not only involving the face and the head, which extend to the ear pinnae and legs. In some cases, hair loss can have a more generalized distribution...
April 2018: Skin Appendage Disorders
https://www.readbyqxmd.com/read/29763680/risk-assessment-of-fluoroquinolones-from-poultry-muscle-consumption-comparing-healthy-adult-and-pre-school-populations
#12
André M P T Pereira, Liliana J G Silva, Jéssica Rodrigues, Celeste Lino, Angelina Pena
Antibiotics, especially fluoroquinolones (FQs), have been largely used in animal husbandry namely poultry production. Therefore, this study aimed to identify, quantify and estimate the daily intake for adult and 3-year-old populations of the FQs norfloxacin (NOR), ciprofloxacin (CIP) and enrofloxacin (ENR) through poultry muscle consumption. The results showed detection frequencies of 78% and 62% in supermarket and school canteen samples, respectively. Of the 182 analysed samples, 4 did not comply with ENR maximum residue level (MRL), and 9 were contaminated with NOR, not allowed as a veterinary medicine of food-producing animals...
May 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29758534/investigations-of-the-corneal-epithelium-in-veterinary-medicine-state-of-the-art-on-corneal-stem-cells-found-in-different-mammalian-species-and-their-putative-application
#13
REVIEW
M Patruno, A Perazzi, T Martinello, C Gomiero, L Maccatrozzo, I Iacopetti
The existence of progenitor cells that can readily differentiate into a specific cell type is a common cellular strategy for physiological tissue growth and repair mechanisms. In the mammalian cornea, many aspects regarding the nature and location of these cells are still unclear. In the human limbus (peripheral area of the cornea) progenitor cells have been found and characterized but in non-human mammals, the picture is not so clear. In this review, we examine current knowledge about the morphology of limbus and the localization of corneal epithelial stem cells in all species studied so far, comparing data with humans...
May 8, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29757668/a-case-of-parasitic-twinning-or-caudal-duplication-in-a-dog
#14
Janet A Grimes, Adrien-Maxence Hespel, Robert C Cole, A Ray Dillon
A 6 mo old 13.5 kg (29.7 lb) male intact American Staffordshire terrier was evaluated for a history of supernumerary pelvic limbs, with malodorous discharge from a supernumerary penis. Imaging (radiographs, abdominal ultrasound, and computed tomography with excretory urogram) showed a supernumerary pelvis with associated pelvic limbs, no osseous continuity with the primary spinal column, a colonic diverticulum extending to the supernumerary pelvis, an enlarged left kidney with a ureter connecting to a single bladder, right renal aplasia, a single descended testicle in the primary scrotum, an intra-abdominal cryptorchid testicle, and two unidentifiable soft tissue masses...
May 14, 2018: Journal of the American Animal Hospital Association
https://www.readbyqxmd.com/read/29755191/26s-proteasome-and-insulin-like-growth-factor-1-in-serum-of-dogs-suffering-from-malignant-tumors
#15
Ingrid Gerke, Franz-Josef Kaup, Stephan Neumann
Studies in humans have shown that the ubiquitin-proteasome pathway and the insulin-like growth factor axis are involved in carcinogenesis, thus, components of these systems might be useful as prognostic markers and constitute potential therapeutic targets. In veterinary medicine, only a few studies exist on this topic. Here, serum concentrations of 26S proteasome (26SP) and insulin-like growth factor-1 (IGF-1) were measured by canine enzyme-linked immunosorbent assay (ELISA) in 43 dogs suffering from malignant tumors and 21 clinically normal dogs (control group)...
April 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/29752373/introducing-evidence-into-practice
#16
(no author information available yet)
Marnie Brennan describes how Nottingham vet school is helping vets embrace evidence-based veterinary medicine.
May 12, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29739469/biomarkers-for-monitoring-intestinal-health-in-poultry-present-status-and-future-perspectives
#17
REVIEW
Richard Ducatelle, Evy Goossens, Fien De Meyer, Venessa Eeckhaut, Gunther Antonissen, Freddy Haesebrouck, Filip Van Immerseel
Intestinal health is determined by host (immunity, mucosal barrier), nutritional, microbial and environmental factors. Deficiencies in intestinal health are associated with shifts in the composition of the intestinal microbiome (dysbiosis), leakage of the mucosal barrier and/or inflammation. Since the ban on growth promoting antimicrobials in animal feed, these dysbiosis-related problems have become a major issue, especially in intensive animal farming. The economical and animal welfare consequences are considerable...
May 8, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29737458/dental-pulp-stem-cells-exploration-in-a-novel-animal-model-the-tasmanian-devil-sarcophilus-harrisii
#18
REVIEW
Chelsea M Graham, Karlea L Kremer, Simon A Koblar, Monica A Hamilton-Bruce, Stephen B Pyecroft
Dental pulp stem cells (DPSC) are a heterogeneous population of highly proliferative stem cells located in the soft inner pulp tissue of the tooth. Demonstrated to have an affinity for neural differentiation, DPSC have been reported to generate functional Schwann cells (SC) through in vitro differentiation. Both DPSC and SC have neural crest origins, recently a significant population of DPSC have been reported to derive from peripheral nerve-associated glia. The predisposition DPSC have towards the SC lineage is not only a very useful tool for neural regenerative therapies in the medical field, it also holds great promise in the veterinary field...
May 8, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29737293/effect-of-sedative-drugs-with-vitamin-b6-combined-with-other-b-vitamins-in-the-anti-stress-of-captive-amur-tiger
#19
Qiao Zhenglei, Ma Jianzhang
In order to reduce the stress response of animals in animal transportation, it is often used to feed the animals with sedative drugs combined with compound vitamin B. In this article, we will randomly divide 16 amur tiger into the control group and the experimental group. The drug intervention is carried out in the experimental group. The sedative is mainly dexmedetomidine, combined with compound vitamin B, while control group did not feed the drug. The urine of two groups of individuals was collected before and after transportation and the cortisol content in urine was determined by radioimmunoassay as an indication of stress...
May 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29735460/effect-of-sedative-drugs-with-vitamin-b6-combined-with-other-b-vitamins-in-the-anti-stress-of-captive-amur-tiger
#20
Qiao Zhenglei, Ma Jianzhang
In order to reduce the stress response of animals in animal transportation, it is often used to feed the animals with sedative drugs combined with compound vitamin B. In this article, we will randomly divide 16 amur tiger into the control group and the experimental group. The drug intervention is carried out in the experimental group. The sedative is mainly dexmedetomidine, combined with compound vitamin B, while control group did not feed the drug. The urine of two groups of individuals was collected before and after transportation and the cortisol content in urine was determined by radioimmunoassay as an indication of stress...
May 2018: Pakistan Journal of Pharmaceutical Sciences
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