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https://www.readbyqxmd.com/read/27913897/rainbow-trout-oncorhynchus-mykiss-pro-oxidant-and-genotoxic-responses-following-acute-and-chronic-exposure-to-the-antibiotic-oxytetracycline
#1
Sara Rodrigues, Sara C Antunes, Alberto T Correia, Bruno Nunes
Oxytetracycline (OTC), an antibacterial agent, is extensively used in aquaculture practices all over the world, but also in human and veterinary medicines. Because of its intensive use, low rates of absorption by treated animals, inadequate disposal, and low efficiency of removal in wastewater treatment plants, the potential harmful effects on aquatic organisms are of great concern. This work aimed to assess the effects of this antibiotic in rainbow trout, following both acute and chronic exposures. Catalase (CAT), total glutathione peroxidase (GPx), glutathione reductase (GRed) activities and lipid peroxidation (TBARS levels) were quantified as oxidative stress biomarkers, in gills and liver...
December 2, 2016: Ecotoxicology
https://www.readbyqxmd.com/read/27911414/isolation-and-culture-of-primary-endothelial-cells-from-canine-arteries-and-veins
#2
Loes A Oosterhoff, Hedwig S Kruitwagen, Bart Spee, Frank G van Steenbeek
Cardiovascular disease is studied in both human and veterinary medicine. Endothelial cells have been used extensively as an in vitro model to study vasculogenesis, (tumor) angiogenesis, and atherosclerosis. The current standard for in vitro research on human endothelial cells (ECs) is the use of Human Umbilical Vein Endothelial Cells (HUVECs) and Human Umbilical Artery Endothelial Cells (HUAECs). For canine endothelial research, only one cell line (CnAOEC) is available, which is derived from canine aortic endothelium...
November 18, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27907059/quantitative-and-selective-analysis-of-feline-growth-related-proteins-using-parallel-reaction-monitoring-high-resolution-mass-spectrometry
#3
Mårten Sundberg, Emma M Strage, Jonas Bergquist, Bodil S Holst, Margareta Ramström
Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM) methods...
2016: PloS One
https://www.readbyqxmd.com/read/27906979/a-phosphorylcholine-containing-glycolipid-like-antigen-present-on-the-surface-of-infective-stage-larvae-of-ascaris-spp-is-a-major-antibody-target-in-infected-pigs-and-humans
#4
Johnny Vlaminck, Dries Masure, Tao Wang, Peter Nejsum, Cornelis H Hokke, Peter Geldhof
BACKGROUND: The pig parasite Ascaris suum plays and important role in veterinary medicine and represents a suitable model for A. lumbricoides, which infects over 800 million people. In pigs, continued exposure to Ascaris induces immunity at the level of the gut, protecting the host against migrating larvae. The objective of this study was to identify and characterize parasite antigens targeted by this local immune response that may be crucial for parasite invasion and establishment and to evaluate their protective and diagnostic potential...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27902913/regulatory-considerations-for-the-approval-of-drugs-against-histomoniasis-blackhead-disease-in-turkeys-chickens-and-game-birds-in-the-united-states
#5
Prajwal R Regmi, Ashley L Shaw, Laura L Hungerford, Janis R Messenheimer, Tong Zhou, Padmakumar Pillai, Amy Omer, Jeffrey M Gilbert
Histomoniasis, commonly referred to as blackhead disease, is a serious threat to the turkey and game bird industries worldwide, and it is having an increasingly negative impact on the chicken industry as well. The Food and Drug Administration's (FDA) Center for Veterinary Medicine (CVM), charged with the approval and regulation of new animal drugs in the United States, understands the rising need for the availability of therapeutic options against histomoniasis. CVM has actively engaged in discussions with the poultry industry, academic institutions, and animal health companies regarding the current status of histomoniasis in the United States and varied success of past and current management, prophylactic, and therapeutic interventions that have been used against the disease...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902406/mlvf-as-a-method-for-rapid-and-cost-effective-typing-of-animal-associated-staphylococcus-aureus-strains-from-lineages-other-than-st398
#6
Maja Kosecka-Strojek, Weronika M Ilczyszyn, Aneta Buda, Klaudia Polakowska, Krzysztof Murzyn, Tomasz Panz, Anna Bialecka, Andrzej Kasprowicz, Antoni Jakubczak, Jaroslaw Krol, Alina Wieliczko, Benedykt Wladyka, Jacek Miedzobrodzki
In veterinary medicine, Staphylococcus aureus is associated with a range of mild to severe infections. The high density of livestock in intensive farming systems increases the risk of disease spread and hampers its control and measures of prevention, making S. aureus one of the most important animal pathogens. Multiple-locus VNTR fingerprinting (MLVF) has been successfully applied to the characterization of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) ST398 but not to the characterization of a wide range of other animal isolates...
October 25, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27899636/the-monarch-initiative-an-integrative-data-and-analytic-platform-connecting-phenotypes-to-genotypes-across-species
#7
Christopher J Mungall, Julie A McMurry, Sebastian Köhler, James P Balhoff, Charles Borromeo, Matthew Brush, Seth Carbon, Tom Conlin, Nathan Dunn, Mark Engelstad, Erin Foster, J P Gourdine, Julius O B Jacobsen, Dan Keith, Bryan Laraway, Suzanna E Lewis, Jeremy NguyenXuan, Kent Shefchek, Nicole Vasilevsky, Zhou Yuan, Nicole Washington, Harry Hochheiser, Tudor Groza, Damian Smedley, Peter N Robinson, Melissa A Haendel
The correlation of phenotypic outcomes with genetic variation and environmental factors is a core pursuit in biology and biomedicine. Numerous challenges impede our progress: patient phenotypes may not match known diseases, candidate variants may be in genes that have not been characterized, model organisms may not recapitulate human or veterinary diseases, filling evolutionary gaps is difficult, and many resources must be queried to find potentially significant genotype-phenotype associations. Non-human organisms have proven instrumental in revealing biological mechanisms...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27896483/the-effects-of-different-carbon-sources-on-the-antifungal-activity-by-lactic-acid-bacteria
#8
Anna Toplaghaltsyan, Inga Bazukyan, Armen Trchounian
Lactobacillus rhamnosus R-2002 strain isolated from Armenian pickled cheese sample has a fungicide effect, which was carried out by the first-time designed method. The fungicide effect of LAB strain was expressed after 5 days of co-cultivation with Mucor plumbeus and 6 days of co-cultivation with Penicillium aurantioviolaceum. The growth as well as the antifungal activity of L. rhamnosus R-2002 was shown to directly depend on the nature of the medium's carbon source. The antifungal activity of this strain against both moulds was inhibited when the combination of 10 g l(-1) glucose and 10 ml l(-1) ethanol in the medium was used as a carbon source...
November 28, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27891118/colistin-in-pig-production-chemistry-mechanism-of-antibacterial-action-microbial-resistance-emergence-and-one-health-perspectives
#9
REVIEW
Mohamed Rhouma, Francis Beaudry, William Thériault, Ann Letellier
Colistin (Polymyxin E) is one of the few cationic antimicrobial peptides commercialized in both human and veterinary medicine. For several years now, colistin has been considered the last line of defense against infections caused by multidrug-resistant Gram-negative such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Colistin has been extensively used orally since the 1960s in food animals and particularly in swine for the control of Enterobacteriaceae infections. However, with the recent discovery of plasmid-mediated colistin resistance encoded by the mcr-1 gene and the higher prevalence of samples harboring this gene in animal isolates compared to other origins, livestock has been singled out as the principal reservoir for colistin resistance amplification and spread...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27890292/principles-and-applications-of-radiation-therapy-in-exotic-animals
#10
REVIEW
Michael S Kent
Radiation therapy is a treatment modality for cancer that is widely used in veterinary medicine, although its use in exotic animal practice has remained limited. However, there are case reports and case series of treating birds, small mammals, and reptiles for a variety of cancers with radiotherapy with varied outcomes. In this article the basic principles of radiation therapy are reviewed and the literature regarding its use in exotic animal practice is summarized. Side effects of radiotherapy are also discussed...
January 2017: Veterinary Clinics of North America. Exotic Animal Practice
https://www.readbyqxmd.com/read/27890291/principles-and-applications-of-surgical-oncology-in-exotic-animals
#11
REVIEW
Michele A Steffey
The diagnosis and treatment of cancer in exotic species is a rapidly evolving area of veterinary medicine. In general, surgical excision remains pivotal in cancer treatment, although optimal outcomes are achieved when a coherent and thorough diagnostic and therapeutic plan is created prior to surgery. While surgical cure is not always achieveable, multimodal treatment plans can offer a variety of options, and palliative procedures may be used to improve quality of life. Treatment goals, whether curative intent or palliative intent, should be identified before surgery, and practitioners should endeavor to adhere to surgical principles in order to attain the best outcomes...
January 2017: Veterinary Clinics of North America. Exotic Animal Practice
https://www.readbyqxmd.com/read/27886775/a-simple-fast-and-sensitive-screening-lc-esi-ms-ms-method-for-antibiotics-in-fish
#12
Letícia Rocha Guidi, Flávio Alves Santos, Ana Cláudia S R Ribeiro, Christian Fernandes, Luiza H M Silva, Maria Beatriz A Gloria
The objective of this study was to develop and validate a fast, sensitive and simple liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the screening of six classes of antibiotics (aminoglycosides, beta-lactams, macrolides, quinolones, sulfonamides and tetracyclines) in fish. Samples were extracted with trichloroacetic acid. LC separation was achieved on a Zorbax Eclipse XDB C18 column and gradient elution using 0.1% heptafluorobutyric acid in water and acetonitrile as mobile phase...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27884143/the-using-of-a-piglets-as-a-model-for-evaluating-the-dipyrone-hematological-effects
#13
Artur Burmańczuk, Andrzej Milczak, Tomasz Grabowski, Monika Osypiuk, Cezary Kowalski
BACKGROUND: Dipyrone (MET, metamizole) is a non-steroidal anti-inflammatory drug commonly used both in human and in veterinary medicine. After oral administration, is broken down rapidly to metabolites which largely retain the activity of the parent drug. Its metabolites have analgesic, antipyretic and anti-inflammatory effects. RESULTS: The subjects were eight healthy male Large White post-suckling piglets, weighing between 5.0 to 7.4 kg, of ages 35 ± 10 days...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27873478/methods-and-processes-of-developing-the-strengthening-the-reporting-of-observational-studies-in-epidemiology-veterinary-strobe-vet-statement
#14
J M Sargeant, A M O'Connor, I R Dohoo, H N Erb, M Cevallos, M Egger, A K Ersbøll, S W Martin, L R Nielsen, D L Pearl, D U Pfeiffer, J Sanchez, M E Torrence, H Vigre, C Waldner, M P Ward
The reporting of observational studies in veterinary research presents many challenges that often are not adequately addressed in published reporting guidelines. A consensus meeting of experts was organized to develop an extension of the STROBE statement to address observational studies in veterinary medicine with respect to animal health, animal production, animal welfare and food safety outcomes. The consensus meeting was held 11-13 May 2014 in Mississauga, Ontario, Canada. Seventeen experts from North America, Europe and Australia attended the meeting...
November 22, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27873166/a-dangerous-hobby-erysipelothrix-rhusiopathiae-bacteremia-most-probably-acquired-from-freshwater-aquarium-fish-handling
#15
E Asimaki, O Nolte, G Overesch, C Strahm
Erysipelothrix rhusiopathiae is a facultative anaerobic Gram-positive rod that occurs widely in nature and is best known in veterinary medicine for causing swine erysipelas. In humans, infections are rare and mainly considered as occupationally acquired zoonosis. A case of E. rhusiopathiae bacteremia most likely associated with home freshwater aquarium handling is reported. The route of transmission was probably a cut with the dorsal fin of a dead pet fish. A short review of clinical presentations, therapeutic considerations and pitfalls of E...
November 21, 2016: Infection
https://www.readbyqxmd.com/read/27872724/are-magnetic-resonance-imaging-or-radiographic-findings-correlated-with-clinical-prognosis-in-spinal-cord-neuropathy
#16
Fatemeh Neshat Halati, Alireza Vajhi, Mohammad Molazem, Mohammad Mehdi Dehghan, Fereshteh Ansari
Dogs presented to the Small Animal Hospital of Veterinary Medicine, University of Tehran were included in the present study if spinal or intervertebral disc involvement was suspected. Clinical signs were recorded as well as general information of the patient such as age, breed and sex. Sixty dogs were examined radiographically and two standard orthogonal lateral and ventrodorsal projections were taken from the suspected region. Then magnetic resonance imaging (MRI) was performed for all patients. Agreement between MRI and radiographic findings, comparison of sex and breed with diagnostic imaging grades, comparison between diagnostic imaging grades and mean age, recovery rate after surgery or medical treatment, effects of diagnostic imaging severity grades on surgical or medical referrals were evaluated statistically...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27860493/biomarkers-in-veterinary-medicine
#17
Michael J Myers, Emily R Smith, Phillip G Turfle
This article summarizes the relevant definitions related to biomarkers; reviews the general processes related to biomarker discovery and ultimate acceptance and use; and finally summarizes and reviews, to the extent possible, examples of the types of biomarkers used in animal species within veterinary clinical practice and human and veterinary drug development. We highlight opportunities for collaboration and coordination of research within the veterinary community and leveraging of resources from human medicine to support biomarker discovery and validation efforts for veterinary medicine...
November 14, 2016: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/27859753/methods-and-processes-of-developing-the-strengthening-the-reporting-of-observational-studies-in-epidemiology-veterinary-strobe-vet-statement
#18
J M Sargeant, A M O'Connor, I R Dohoo, H N Erb, M Cevallos, M Egger, A K Ersbøll, S W Martin, L R Nielsen, D L Pearl, D U Pfeiffer, J Sanchez, M E Torrence, H Vigre, C Waldner, M P Ward
BACKGROUND: Reporting of observational studies in veterinary research presents challenges that often are not addressed in published reporting guidelines. OBJECTIVE: To develop an extension of the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) statement that addresses unique reporting requirements for observational studies in veterinary medicine related to health, production, welfare, and food safety. DESIGN: Consensus meeting of experts...
November 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27859751/subclinical-bacteriuria-in-older-cats-and-its-association-with-survival
#19
J D White, N J Cave, A Grinberg, D G Thomas, C Heuer
BACKGROUND: Bacterial urinary tract infections are uncommon in cats in general but the prevalence increases to 29% in older cats with comorbidities (Veterinary Clinical Pathology 2008, 37, 317; Journal of Feline Medicine & Surgery 2007, 9, 124; Veterinary Microbiology 2009, 136, 130). Frequently, the infections are subclinical. The clinical relevance of subclinical bacteriuria (SB) is uncertain, and the optimal treatment requires clarification. OBJECTIVE: Prospective, observational study to: (i) identify the prevalence and incidence count of SB in older (≥7 years), nonazotemic cats, (ii) evaluate specific risk factors for SB, and (iii) investigate the potential relationship between untreated SB and survival...
November 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27859688/normalized-resistance-interpretation-the-nri-method-review-of-nri-disc-test-applications-and-guide-to-calculations
#20
REVIEW
Göran Kronvall, Peter Smith
The normalized resistance interpretation (NRI) method was developed in response to a call for a method to calibrate disc diffusion test results making inter-laboratory comparisons possible. The main use of NRI so far has been in individual laboratories, in medical and veterinary medicine and in the field of marine microbiology. The applications of NRI for disc diffusion tests are reviewed and, in addition, a detailed description of the calculation procedure is presented. NRI provides a fully objective method for ECOFF calculations of disc diffusion antimicrobial susceptibility test results...
December 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
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