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https://www.readbyqxmd.com/read/29353412/helicobacter-pylori-lps-induced-gastric-mucosal-spleen-tyrosine-kinase-syk-recruitment-to-tlr4-and-activation-occurs-with-the-involvement-of-protein-kinase-c%C3%AE
#1
B L Slomiany, A Slomiany
Spleen tyrosine kinase (Syk) has emerged recently as a major effector of proinflammatory genes expression induced by LPS-elicited TLR4 activation, and manifested by the up-amplification in the production of inflammatory mediators, including PGE2 and NO. Here, we investigated the nature of factors involved in the recruitment and interaction of Syk with TLR4 in gastric mucosa in response to H. pylori LPS. We show that stimulation of gastric mucosal cells with the LPS leads to localization of Syk with the membrane-associated TLR4 complex and its activation through phosphorylation on Tyr...
January 20, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29353348/insulin-receptor-substrate-4-is-overexpressed-in-colorectal-cancer-and-promotes-retinoblastoma-cyclin-dependent-kinase-activation
#2
Patricia Sanmartín-Salinas, María Del Val Toledo Lobo, Fernando Noguerales-Fraguas, Miguel Toro Londoño, Antonio Jiménez-Ruiz, Luis Gonzalez Guijarro
BACKGROUND: Insulin receptor substrate 4 (IRS-4) is an adaptor protein for which new evidence suggests plays a role in tumour promotion. METHODS: We described nuclear IRS-4 in RKO colon cancer cell lines in biopsies of patients with colorectal cancer (CRC) (n = 20) and in matched adjacent normal colorectal (MANC) tissue (n = 20). RESULTS: Treatment with physiological doses of IGF-1 promoted nuclear influx of IRS-4 from cellular cytosol in RKO cells...
January 20, 2018: Journal of Gastroenterology
https://www.readbyqxmd.com/read/29353240/pip4k2a-regulates-intracellular-cholesterol-transport-through-modulating-pi-4-5-p2-homeostasis
#3
Ao Hu, Xue-Tong Zhao, Heng Tu, Ting Xiao, Ting Fu, Yan Wang, Yong Liu, Xiong-Jie Shi, Jie Luo, Bao-Liang Song
The transport of LDL-derived cholesterol from lysosomes to peroxisomes is mediated by membrane contacts, which are facilitated by the lysosomal protein synaptotagmin VII and the peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2). Here, we used RNA interference to search for regulators of PI(4,5)P2 and to study the effects of altered PI(4,5)P2 homeostasis on cholesterol transport. We found that knockdown of phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) reduced peroxisomal PI(4,5)P2 levels, decreased lysosome-peroxisome membrane contacts, and increased accumulation of lysosomal cholesterol in human SV-589 fibroblasts...
January 20, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29353142/mirna-185-regulates-the-vegfa-signaling-pathway-in-dairy-cows-with-retained-fetal-membranes
#4
REVIEW
C Y Zheng, X Zou, H J Lin, B C Zhao, M L Zhang, C H Luo, S X Fu
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations at 6, 12, and 24 h after calving...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353140/development-of-sea-bream-sparus-aurata-semen-vitrification-protocols
#5
Loredana Zilli, Annalisa Bianchi, Maroua Sabbah, Laura Pecoraro, Roberta Schiavone, Sebastiano Vilella
The long-term goal of this research project is to set up efficient protocol that can be used to develop a standardized approach for vitrification of marine fish spermatozoa. In particular, the aim of the present study was to develop a vitrification protocol for sea bream (Sparus aurata) spermatozoa. To draw up the protocol, we tested two different dilution media (1% NaCl and Mounib medium), three different vitrification devices (loops, drops and cut straws), different cryoprotectants (CPs) and three different equilibration times (30, 60 and 120 s)...
January 11, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353001/membrane-progesterone-receptors-%C3%AE-and-%C3%AE-have-potential-as-prognostic-biomarkers-of-endometrial-cancer
#6
Maša Sinreih, Tamara Knific, Peter Thomas, Snježana Frković Grazio, Tea Lanišnik Rižner
Endometrial cancer (EC) is one of the most common malignancies in women worldwide. EC is linked to chronic exposure to estrogens that is unopposed by protective effects of progesterone. Progesterone modulates gene expression via classical nuclear receptors, and has rapid effects via the less characterized membrane-bound progesterone receptors (mPRs) of the progestin and adipoQ receptor (PAQR) family. The presence of mPRs in EC has not been investigated to date. The aims of this study were to examine PAQR7, PAQR8 and PAQR5, which encode for mPRα, mPRβ and mPRγ, respectively, for their expression and localization in EC tissue and adjacent control endometrium...
January 15, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29352896/biological-activities-and-pharmaceutical-applications-of-polysaccharide-from-natural-resources-a-review
#7
REVIEW
Yue Yu, Mingyue Shen, Qianqian Song, Jianhua Xie
Pharmacotherapy using natural substances can be currently regarded as a very promising future alternative to conventional therapy. As biological macromolecules, polysaccharide together with protein and polynucleotide, are extremely important biomacromoleules which play important roles in the growth and development of living organism. Polysaccharide is important component of higher plants, membrane of the animal cell and the cell wall of microbes. It is also closely related to the physiological functions. Recently, increasing attention has been paid on polysaccharides as an important class of bioactive natural products...
March 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352746/the-common-bean-cok-4-and-the-arabidopsis-fer-kinase-domain-share-similar-function-in-plant-growth-and-defense
#8
Rafhael Felipin Azevedo, Maria Celeste Gonçalves Vidigal, Paula Rodrigues Oblessuc, Maeli Melotto
Receptor-like kinases are membrane proteins that can be shared by diverse signaling pathways. Among them, the Arabidopsis thaliana FERONIA (FER) plays a role in the balance between distinct signals to control growth and defense. We have found that COK-4, a putative kinase encoded in the common bean anthracnose resistance locus Co-4 that is transcriptionally regulated during immune response, is highly similar to the kinase domain of FER. To assess whether COK-4 is a functional ortholog of FER, we expressed COK-4 in the wild type Col-0 and the fer-5 mutant of Arabidopsis and evaluated FER-associated traits...
January 20, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29352733/localization-of-palmitoylated-and-activated-g-protein-%C3%AE-subunit-in-dictyostelium-discoideum
#9
Sarah Alamer, Yusuke Kageyama, Robert E Gundersen
Guanine nucleotide-binding proteins (G proteins) act as molecular switches to regulate many fundamental cellular processes. The lipid modification, palmitoylation, can be considered as a key factor for proper G protein function and plasma membrane localization. In Dictyostelium discoidum, Gα2 is essential for the chemotactic response to cAMP in their developmental life cycle. However, the regulation of Gα2 with respect to palmitoylation, activation and Gβγ association is less clear. In this study, Gα2 is shown to be palmitoylated on Cys-4 by [3 H]palmitate labeling...
January 20, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29352507/nuclear-and-membrane-estrogen-receptor-antagonists-induce-similar-mtorc2-activation-reversible-changes-in-synaptic-protein-expression-and-actin-polymerization-in-the-mouse-hippocampus
#10
Fang-Zhou Xing, Yan-Gang Zhao, Yuan-Yuan Zhang, Li He, Ji-Kai Zhao, Meng-Ying Liu, Yan Liu, Ji-Qiang Zhang
AIMS: Estrogens play pivotal roles in hippocampal synaptic plasticity through nuclear receptors (nERs; including ERα and ERβ) and the membrane receptor (mER; also called GPR30), but the underlying mechanism and the contributions of nERs and mER remain unclear. Mammalian target of rapamycin complex 2 (mTORC2) is involved in actin cytoskeleton polymerization and long-term memory, but whether mTORC2 is involved in the regulation of hippocampal synaptic plasticity by ERs is unclear. METHODS: We treated animals with nER antagonists (MPP/PHTPP) or the mER antagonist (G15) alone or in combination with A-443654, an activator of mTORC2...
January 19, 2018: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29352487/disaccharide-combinations-and-the-expression-of-enolase3-and-plasma-membrane-ca2-atpase-isoform-in-sturgeon-sperm-cryopreservation
#11
M D Xi, P Li, H Du, X M Qiao, Z G Liu, Q W Wei
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A...
January 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29352483/epidermal-aspects-of-type-vii-collagen-implications-for-dystrophic-epidermolysis-bullosa-and-epidermolysis-bullosa-acquisita
#12
REVIEW
Mika Watanabe, Ken Natsuga, Satoru Shinkuma, Hiroshi Shimizu
Type VII collagen (COL7), a major component of anchoring fibrils in the epidermal basement membrane zone, has been characterized as a defective protein in dystrophic epidermolysis bullosa and as an autoantigen in epidermolysis bullosa acquisita. Although COL7 is produced and secreted by both epidermal keratinocytes and dermal fibroblasts, the role of COL7 with regard to the epidermis is rarely discussed. This review focuses on COL7 physiology and pathology as it pertains to epidermal keratinocytes. We summarize the current knowledge of COL7 production and trafficking, its involvement in keratinocyte dynamics, and epidermal carcinogenesis in COL7 deficiency and propose possible solutions to unsolved issues in this field...
January 20, 2018: Journal of Dermatology
https://www.readbyqxmd.com/read/29352432/effect-of-membrane-composition-on-receptor-association-implications-of-cancer-lipidomics-on-erbb-receptors
#13
REVIEW
Aiswarya B Pawar, Durba Sengupta
The association of single transmembrane receptors, such as the ErbB receptors is a key event in initiating cell signaling networks. The interactions between these receptors have been well characterized for both ligand-driven and pre-formed dimers. However, the role of the membrane in modulating association is less well understood and assumes greater importance in light of altered membrane composition in diseased states. Here, we discuss how membrane composition has been observed to induce both structural and dynamic differences in receptor association...
January 19, 2018: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29352320/high-level-production-and-purification-in-a-functional-state-of-an-extrasynaptic-gamma-aminobutyric-acid-type-a-receptor-containing-%C3%AE-4%C3%AE-3%C3%AE-subunits
#14
Xiaojuan Zhou, Rooma Desai, Yinghui Zhang, Wojciech J Stec, Keith W Miller, Youssef Jounaidi
The inhibitory γ-aminobutyric acid type A receptors are implicated in numerous physiological processes, including cognition and inhibition of neurotransmission, rendering them important molecular targets for many classes of drugs. Functionally, the entire GABAAR family of receptors can be subdivided into phasic, fast acting synaptic receptors, composed of α-, β- and γ-subunits, and tonic extrasynaptic receptors, many of which contain the δ-subunit in addition to α- and β-subunits. Whereas the subunit arrangement of the former group is agreed upon, that of the αβδ GABAARs remains unresolved by electrophysiological and pharmacological research...
2018: PloS One
https://www.readbyqxmd.com/read/29352252/towards-an-experimental-classification-system-for-membrane-active-peptides
#15
G D Brand, M H S Ramada, T C Genaro-Mattos, C Bloch
Mature proteins can act as potential sources of encrypted bioactive peptides that, once released from their parent proteins, might interact with diverse biomolecular targets. In recent work we introduced a systematic methodology to uncover encrypted intragenic antimicrobial peptides (IAPs) within large protein sequence libraries. Given that such peptides may interact with membranes in different ways, resulting in distinct observable outcomes, it is desirable to develop a predictive methodology to categorize membrane active peptides and establish a link to their physicochemical properties...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352251/differential-effects-of-sumo1-and-sumo3-on-pkr-activation-and-stability
#16
Ghizlane Maarifi, Faten El Asmi, Mohamed Ali Maroui, Laurent Dianoux, Mounira K Chelbi-Alix
Double-stranded RNA (dsRNA)-dependent protein kinase (PKR) is a serine/threonine kinase that exerts its own phosphorylation and the phosphorylation of the α subunit of the protein synthesis initiation factor eIF-2α. PKR was identified as a target of SUMOylation and the triple PKR-SUMO deficient mutant on Lysine residues K60-K150-K440 has reduced PKR activity. We report that SUMO1 and SUMO3 expression exert differential effects on PKR localization, activation and stability. SUMO1 or SUMO3 did not alter the repartition of PKR in the cytoplasm and the nucleus...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352235/systems-modeling-accurately-predicts-responses-to-genotoxic-agents-and-their-synergism-with-bcl-2-inhibitors-in-triple-negative-breast-cancer-cells
#17
Federico Lucantoni, Andreas U Lindner, Norma O'Donovan, Heiko Düssmann, Jochen H M Prehn
Triple negative breast cancer (TNBC) is an aggressive form of breast cancer which accounts for 15-20% of this disease and is currently treated with genotoxic chemotherapy. The BCL2 (B-cell lymphoma 2) family of proteins controls the process of mitochondrial outer membrane permeabilization (MOMP), which is required for the activation of the mitochondrial apoptosis pathway in response to genotoxic agents. We previously developed a deterministic systems model of BCL2 protein interactions, DR_MOMP that calculates the sensitivity of cells to undergo mitochondrial apoptosis...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29352166/epstein-barr-virus-encoded-latent-membrane-protein-1-suppresses-necroptosis-through-targeting-ripk1-3-ubiquitination
#18
Xiaolan Liu, Yueshuo Li, Songling Peng, Xinfang Yu, Wei Li, Feng Shi, Xiangjian Luo, Min Tang, Zheqiong Tan, A M Bode, Ya Cao
Necroptosis is an alternative programmed cell death pathway that is unleashed in the absence of apoptosis and mediated by signaling complexes containing receptor-interating protein kinase 1 (RIPK1) and RIPK3. This form of cell death has recently been implicated in host defense system to eliminate pathogen-infected cells. However, only a few viral species such as herpes simplex virus (HSV) and cytomegalovirus (CMV) have evolved mechanisms inhibiting necroptosis to overcome host antiviral defense, which is important for successful pathogenesis...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29352138/mir-663-sustains-nsclc-by-inhibiting-mitochondrial-outer-membrane-permeabilization-momp-through-puma-bbc3-and-btg2
#19
Micol E Fiori, Lidia Villanova, Chiara Barbini, Maria Laura De Angelis, Ruggero De Maria
Treatment of lung cancer is an unmet need as it accounts for the majority of cancer deaths worldwide. The development of new therapies urges the identification of potential targets. MicroRNAs' expression is often deregulated in cancer and their modulation has been proposed as a successful strategy to interfere with tumor cell growth and spread. We recently reported on an unbiased high-content approach to identify miRNAs regulating cell proliferation and tumorigenesis in non-small cell lung cancer (NSCLC). Here we studied the oncogenic role of miR-663 in NSCLC biology and analyzed the therapeutic potential of miR-663 targeting...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29352104/a-ph-like-domain-of-the-rab12-guanine-nucleotide-exchange-factor-dennd3-binds-actin-and-is-required-for-autophagy
#20
Jie Xu, Guennadi Kozlov, Peter S McPherson, Kalle Gehring
Rab GTPases are key regulators of membrane trafficking and many are activated by guanine nucleotide exchange factors bearing a <span style="text-decoration: underline;">d</span>ifferentially <span style="text-decoration: underline;">e</span>xpressed in <span style="text-decoration: underline;">n</span>ormal and <span style="text-decoration: underline;">n</span>eoplastic cells (DENN) domain. By activating the small GTPase Rab12, DENN domain-containing protein 3 (DENND3) functions in autophagy...
January 19, 2018: Journal of Biological Chemistry
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