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https://www.readbyqxmd.com/read/28527130/pulse-generation-in-the-quorum-machinery-of-pseudomonas-aeruginosa
#1
Cicik Alfiniyah, Martin A Bees, A Jamie Wood
Pseudomonas aeruginosa is a Gram-negative bacterium that is responsible for a wide range of infections in humans. Colonies employ quorum sensing (QS) to coordinate gene expression, including for virulence factors, swarming motility and complex social traits. The QS signalling system of P. aeruginosa is known to involve multiple control components, notably the las, rhl and pqs systems. In this paper, we examine the las system and, in particular, the repressive interaction of rsaL, an embedded small regulative protein, employing recent biochemical information to aid model construction...
May 19, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28527125/diversity-and-probiotic-potential-of-lactic-acid-bacteria-isolated-from-horreh-a-traditional-iranian-fermented-food
#2
Alireza Vasiee, Behrooz Alizadeh Behbahani, Farideh Tabatabaei Yazdi, Seyed Ali Mortazavi, Hamid Noorbakhsh
The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) strains isolated from Horreh. Some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. A total of 140 Gram-positive and catalase-negative isolates from Horreh were subjected to identification and grouping by cultural methods and the 16S rRNA sequencing. The new isolates were identified to be Lactobacillus (fermentum, plantarum, and brevis) Weissella cibaria, Enterococcus (faecium and faecalis), Leuconostoc (citreum and mesenteroides subsp...
May 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28526895/three-novel-xenorhabdus-steinernema-associations-and-evidence-of-strains-of-x-khoisanae-switching-between-different-clades
#3
Jonike Dreyer, Antoinette P Malan, Leon M T Dicks
Xenorhabdus species are normally closely associated with entomopathogenic nematodes of the family Steinernematidae. Strain F2, isolated from Steinernema nguyeni, was identified as Xenorhabdus bovienii and strains J194 and SB10, isolated from Steinernema jeffreyense and Steinernema sacchari as Xenorhabdus khoisanae, based on phenotypic characteristics and sequencing of 16S rRNA and housekeeping genes dnaN, gltX, gyrB, infB and recA. All three strains produced antimicrobial compounds that inhibited the growth of Gram-positive and Gram-negative bacteria...
May 19, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28526864/a-broad-spectrum-bactericidal-lipopeptide-with-anti-biofilm-properties
#4
Ohad Meir, Fadia Zaknoon, Uri Cogan, Amram Mor
Previous studies of the oligoacyllysyl (OAK) series acyl-lysyl-lysyl-aminoacyl-lysine-amide, suggested their utility towards generating robust linear lipopeptide-like alternatives to antibiotics, although to date, none exhibited potent broad-spectrum bactericidal activity. To follow up on this premise, we produced a new analog (C14KKc12K) and investigated its properties in various media. Mechanistic studies suggest that C14KKc12K uses a non-specific membrane-disruptive mode of action for rapidly reducing viability of Gram-negative bacteria (GNB) similarly to polymyxin B (PMB), a cyclic lipopeptide used as last resort antibiotic...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526845/chemical-structure-of-the-lipid-a-component-of-pseudomonas-sp-strain-pamc-28618-from-thawing-permafrost-in-relation-to-pathogenicity
#5
Han-Gyu Park, Ganesan Sathiyanarayanan, Cheol-Hwan Hwang, Da-Hee Ann, Jung-Ho Kim, Geul Bang, Kyoung-Soon Jang, Hee Wook Ryu, Yoo Kyung Lee, Yung-Hun Yang, Yun-Gon Kim
Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MS(n)). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526789/a-novel-iron-dependent-enzyme-that-catalyzes-the-initial-step-in-the-biodegradation-of-n-nitroglycine-by-variovorax-sp-strain-js1663
#6
Kristina M Mahan, Hangping Zheng, Tekle T Fida, Ronald J Parry, David E Graham, Jim C Spain
Nitramines are key constituents of most of the explosives in current use and consequently contaminate soil and groundwater at many military facilities around the world. Toxicity from nitramine contamination poses a health risk to plants and animals, thus understanding how nitramines are biodegraded is critical to environmental remediation. The biodegradation of synthetic nitramine compounds such as hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX) has been studied for decades, but little is known about the catabolism of naturally produced nitramine compounds...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526637/identification-and-biological-activity-of-the-volatile-compounds-of-glycyrrhiza-triphylla-fisch-c-a-mey
#7
Abolfazl Shakeri, Javad Akhtari, Vahid Soheili, Seyedeh Faezeh Taghizadeh, Amirhossein Sahebkar, Rezvan Shaddel, Javad Asili
Chemical composition and biological (antimicrobial, antioxidant and cytotoxic) activities of essential oils (EO) obtained from the aerial parts of Glycyrrhiza triphylla Fisch. & C.A.Mey (G. triphylla) were evaluated in the present study. The EO was isolated and analyzed using gas chromatography-mass spectrometry (GC-MS). Fifty-five compounds representing 99.3% of the total oil composition were identified. Major components of the oil were β-caryophyllene (25.4%), limonene (16.7%), β-myrcene (16.0%) and α-humulene (4...
May 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28526515/prenatal-lipopolysaccharide-induces-hypothalamic-dopaminergic-hypoactivity-and-autistic-like-behaviors-repetitive-self-grooming-and-stereotypies
#8
Thiago B Kirsten, Maria M Bernardi
Previous investigations by our group have shown that prenatal exposure to lipopolysaccharide (LPS), which mimics infection by gram-negative bacteria, induces social, cognitive, and communication deficits. For a complete screening of autistic-like behaviors, the objective of this study was to evaluate if our rat model also induces restricted and repetitive stereotyped behaviors. Thus, we studied the self-grooming microstructure. We also studied the neurochemistry of hypothalamus and frontal cortex, which are brain areas related to autism to better understand central mechanisms involved in our model...
May 16, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28526080/post-weaning-diarrhea-in-pigs-risk-factors-and-non-colistin-based-control-strategies
#9
REVIEW
Mohamed Rhouma, John Morris Fairbrother, Francis Beaudry, Ann Letellier
Post-weaning diarrhea (PWD) is one of the most serious threats for the swine industry worldwide. It is commonly associated with the proliferation of enterotoxigenic Escherichia coli in the pig intestine. Colistin, a cationic antibiotic, is widely used in swine for the oral treatment of intestinal infections caused by E. coli, and particularly of PWD. However, despite the effectiveness of this antibiotic in the treatment of PWD, several studies have reported high rates of colistin resistant E. coli in swine...
May 19, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28525997/increased-resistance-of-gram-negative-urinary-pathogens-after-kidney-transplantation
#10
Johannes Korth, Julia Kukalla, Peter-Michael Rath, Sebastian Dolff, Marco Krull, Hana Guberina, Anja Bienholz, Benjamin Wilde, Stefan Becker, Birgit Ross, Olympia Evdoxia Anastasiou, Andreas Kribben, Oliver Witzke
BACKGROUND: Urinary tract infection is the most common complication after kidney transplantation. It can cause severe sepsis and transplant loss. Emergence of drug resistance among gram-negative urinary pathogens is the current challenge for urinary tract infection treatment after kidney transplantation. METHODS: This study analyzes the antimicrobial susceptibility of gram-negative urinary pathogens after kidney transplantation from 2009 to 2012 at the Transplant Outpatient Clinic of the University Hospital Essen, Germany...
May 19, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28525777/prevalence-and-clinical-significance-of-early-high-endotoxin-activity-in-septic-shock-an-observational-study
#11
Maurizio Bottiroli, Gianpaola Monti, Riccardo Pinciroli, Irene Vecchi, Valeria Terzi, Giuseppe Ortisi, Giampaolo Casella, Roberto Fumagalli
PURPOSE: To measure the prevalence of elevated Endotoxin Activity (EA) in a large cohort of patients with Septic Shock (SS), and to assess its value as an early indicator of Gram-Negative (GN) infection, disease severity, and patient risk. MATERIALS AND METHODS: Adult patients were enrolled in this observational study if an EA determination was obtained within 24-h from SS onset. Demographic, clinical, and microbiological data were collected. In-hospital follow-up was also conducted...
April 26, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28524087/in-vitro-assessment-of-early-bacterial-activity-on-micro-nanostructured-ti6al4v-surfaces
#12
Benjamin Valdez-Salas, Ernesto Beltrán-Partida, Sandra Castillo-Uribe, Mario Curiel-Álvarez, Roumen Zlatev, Margarita Stoytcheva, Gisela Montero-Alpírez, Lidia Vargas-Osuna
It is imperative to understand and systematically compare the initial interactions between bacteria genre and surface properties. Thus, we fabricated a flat, anodized with 80 nm TiO₂ nanotubes (NTs), and a rough Ti6Al4V surface. The materials were characterized using field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). We cultured in vitro Staphylococcus epidermidis (S. epidermidis) and Pseudomonas aeruginosa (P. aeruginosa) to evaluate the bacterial-surface behavior by FE-SEM and viability calculation...
May 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28522994/new-potent-membrane-targeting-antibacterial-peptides-from-viral-capsid-proteins
#13
Susana A Dias, João M Freire, Clara Pérez-Peinado, Marco M Domingues, Diana Gaspar, Nuno Vale, Paula Gomes, David Andreu, Sónia T Henriques, Miguel A R B Castanho, Ana S Veiga
The increasing prevalence of multidrug-resistant bacteria urges the development of new antibacterial agents. With a broad spectrum activity, antimicrobial peptides have been considered potential antibacterial drug leads. Using bioinformatic tools we have previously shown that viral structural proteins are a rich source for new bioactive peptide sequences, namely antimicrobial and cell-penetrating peptides. Here, we test the efficacy and mechanism of action of the most promising peptides among those previously identified against both Gram-positive and Gram-negative bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28522905/strategies-used-by-helicobacter-pylori-to-establish-persistent-infection
#14
REVIEW
Amin Talebi Bezmin Abadi
Helicobacter pylori (H. pylori) is a Gram-negative and motile bacterium that colonizes the hostile microniche of the human stomach, then persists for the host's entire life, if not effectively treated. Clinically, H. pylori plays a causative role in the development of a wide spectrum of diseases including chronic active gastritis, peptic ulceration, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma. Due to the global distribution of H. pylori, it is no exaggeration to conclude that smart strategies are contributing to adaptation of the bacterium to its permanent host...
April 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28522802/proteome-remodelling-by-the-stress-sigma-factor-rpos-%C3%AF-s-in-salmonella-identification-of-small-proteins-and-evidence-for-post-transcriptional-regulation
#15
Magali Lago, Véronique Monteil, Thibaut Douche, Julien Guglielmini, Alexis Criscuolo, Corinne Maufrais, Mariette Matondo, Françoise Norel
The RpoS/σ(S) sigma subunit of RNA polymerase is the master regulator of the general stress response in many Gram-negative bacteria. Extensive studies have been conducted on σ(S)-regulated gene expression at the transcriptional level. In contrast, very limited information regarding the impact of σ(S) on global protein production is available. In this study, we used a mass spectrometry-based proteomics approach to explore the wide σ(S)-dependent proteome of the human pathogen Salmonella enterica serovar Typhimurium...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28522607/nleb-ssek-effectors-from-citrobacter-rodentium-escherichia-coli-and-salmonella-enterica-display-distinct-differences-in-host-substrate-specificity
#16
Samir El Qaidi, Kangming Chen, Adnan Halim, Lina Siukstaite, Christian Rueter, Ramon Hurtado-Guerrero, Henrik Clausen, Philip R Hardwidge
Many Gram-negative bacterial pathogens use a syringe-like apparatus called a type III secretion system to inject virulence factors into host cells. Some of these effectors are enzymes that modify host proteins to subvert their normal functions. NleB is a glycosyltransferase that modifies host proteins with N-acetyl-D-glucosamine to inhibit antibacterial and inflammatory host responses. NleB is conserved among the attaching/effacing pathogens enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), and Citrobacter rodentium...
May 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28522212/bioengineering-bacterial-outer-membrane-vesicles-as-vaccine-platform
#17
REVIEW
Matthias J H Gerritzen, Dirk E Martens, René H Wijffels, Leo van der Pol, Michiel Stork
Outer membrane vesicles (OMVs) are naturally non-replicating, highly immunogenic spherical nanoparticles derived from Gram-negative bacteria. OMVs from pathogenic bacteria have been successfully used as vaccines against bacterial meningitis and sepsis among others and the composition of the vesicles can easily be engineered. OMVs can be used as a vaccine platform by engineering heterologous antigens to the vesicles. The major advantages of adding heterologous proteins to the OMV are that the antigens retain their native conformation, the ability of targeting specific immune responses, and a single production process suffices for many vaccines...
May 15, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28522160/complete-genome-of-a-metabolically-diverse-marine-bacterium-shewanella-japonica-kctc-22435-t
#18
Kyung Mo Kim, Hanna Choe, Byung Kwon Kim, Arshan Nasir
Shewanella japonica KCTC 22435(T) is a facultatively anaerobic, Gram-negative, mesophilic, rod-shaped bacterium isolated from sea water at the Pacific Institute of Bio-organic Chemistry of the Marine Experimental Station, Troitza Bay, Gulf of Peter the Great, Russia. Here, we report the complete genome of S. japonica KCTC 22435(T), which consists of 4,975,677bp (G+C content of 40.80%) with a single chromosome, 4036 protein-coding genes, 97 tRNAs and 8 rRNA operons. Genes detected in the genome reveal that the strain possesses a type II secretion system, cytochrome c family proteins with various numbers of heme-binding motifs, and metabolic pathways for utilizing diverse carbon sources, supporting the potential of KCTC 22435(T) to generate electricity in salinity culture conditions...
May 15, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28522007/guar-gum-benzoate-nanoparticle-reinforced-gelatin-films-for-enhanced-thermal-insulation-mechanical-and-antimicrobial-properties
#19
Sonia Kundu, Aatrayee Das, Aalok Basu, Md Farooque Abdullah, Arup Mukherjee
This work relates to guar gum benzoate self assembly nanoparticles synthesis and nano composite films development with gelatin. Guar gum benzoate was synthesized in a Hofmeister cation guided homogeneous phase reaction. Self assembly polysaccharide nanoparticles were prepared in solvent displacement technique. Electron microscopy and DLS study confirmed uniform quasi spherical nanoparticles with ζ-potential - 28.7mV. Nanocomposite films were further developed in gelatin matrix. The film capacity augmenting due to nanoparticles incorporation was noteworthy...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521337/perspectives-for-clinical-use-of-engineered-human-host-defense-antimicrobial-peptides
#20
María Eugenia Pachón-Ibáñez, Younes Smani, Jerónimo Pachón, Javier Sánchez-Céspedes
Infectious diseases caused by bacteria, viruses or fungi are among the leading causes of death worldwide. The emergence of drug-resistance mechanisms, especially among bacteria, threatens the efficacy of all current antimicrobial agents, some of them already ineffective. As a result, there is an urgent need for new antimicrobial drugs. Host defense antimicrobial peptides (HDPs) are natural occurring and well-conserved peptides of innate immunity, broadly active against Gram-negative and Gram-positive bacteria, viruses and fungi...
May 1, 2017: FEMS Microbiology Reviews
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