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https://www.readbyqxmd.com/read/28441623/identification-of-a-macrobrachium-nipponense-c-type-lectin-with-a-close-evolutionary-relationship-to-vertebrate-lectins
#1
Xin Huang, Tingting Li, Min Jin, Shaowu Yin, Wen Wang, Qian Ren
C-type lectins (CTLs) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. This study cloned and characterized a novel C-type lectin (MnCTL) of the oriental river prawn, Macrobrachium nipponense. The cloned MnCTL cDNA encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. The deduced MnCTL protein contains a single carbohydrate recognition domain (CRD) with an EPN (Glu-Pro-Asn) motif in calcium-binding site 2. Phylogenetic analysis indicated that MnCTL has a closer evolutionary relationship with vertebrate lectins than with invertebrate lectins...
April 22, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28441606/growth-inhibition-and-antibiofilm-potential-of-ag-nanoparticles-coated-with-lectin-an-arthropod-immune-molecule
#2
Sangily Jayanthi, Sathappan Shanthi, Baskaralingam Vaseeharan, Narayanan Gopi, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. Here, lectin was purified from the haemolymph of blue swimmer crab Portunus pelagicus, using mannose-coupled sepharose CL-4B affinity column chromatography. In SDS-PAGE analysis, lectin showed a molecular mass of approximately 155kDa. The synthesis of lectin-coated silver nanoparticles (lectin-coated AgNPs) was confirmed by UV-Vis spectroscopy, XRD, FTIR, SEM, TEM, SAED, and EDX analysis...
April 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28441577/membrane-vesicles-and-horizontal-gene-transfer-in-prokaryotes
#3
REVIEW
Sara Domingues, Kaare M Nielsen
Membrane vesicles (MVs) are released from all living cells. MVs are lumen-containing spheres of lipid-bilayers derived from the cell surface. MVs are biologically active and contain various components, including genetic material. Both chromosomal and plasmid DNA, as well as different types of RNA have been detected in MVs. Vesicle-mediated transfer of genes coding for antibiotic resistance, virulence and metabolic traits has been reported in Gram-negative and Gram-positive bacteria and in Archaea. MVs can persist over time in natural environments...
April 22, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28441516/epoxynemanione-a-nemanifuranones-a-f-and-nemanilactones-a-c-from-nemania-serpens-an-endophytic-fungus-isolated-from-riesling-grapevines
#4
Ashraf Ibrahim, Dan Sørensen, Hilary A Jenkins, Linda Ejim, Alfredo Capretta, Mark W Sumarah
Ten polyketide specialized metabolites, epoxynemanione A, nemanifuranones A-F, and nemanilactones A-C, were isolated from the culture filtrate of Nemania serpens (Pers.) Grey (1821), an endophytic fungus from a Riesling grapevine (Vitis vinifera) found in Canada's Niagara region. Additionally, four known metabolites 2-(hydroxymethyl)-3-methoxy-benzoic acid, phyllostine, 5-methylmellein and a nordammarane triterpenoid were isolated. A related known metabolite 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one has also been included for structural and biological comparison to the nemanifuranones...
April 22, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28441365/efficacy-and-safety-of-3-nasal-packing-materials-used-after-functional-endoscopic-sinus-surgery-for-chronic-rhinosinusitis-a-comparative-study-in-china
#5
Xi-Ling Zheng, Yu-Xiang Zhao, Min Xu
BACKGROUND This study aimed to investigate the efficacy and safety of 3 nasal packing products (silicone tube, Beschitin-F, and Aquacel1-Ag) after functional endoscopic sinus surgery (FESS) for chronic rhinosinusitis (CRS) patients in China. MATERIAL AND METHODS Sixty-six CRS patients undergoing FESS surgery were randomly divided into 3 groups (22 patients in each group): the silicone tube group, the Beschitin-F group, and the Aquacel1-Ag group. Postoperative headache, nasal pain, nasal bleeding, and swelling of the nasal mucosa were observed at 1 month after FESS surgery...
April 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28441310/a-dose-response-curve-for-a-gram-negative-spinal-implant-infection-model-in-rabbits
#6
Joseph L Laratta, Jamal N Shillingford, Nathan Hardy, Ronald A Lehman, Lawrence G Lenke, K Daniel Riew
STUDY DESIGN: Randomized complete block animal spinal implant infection model with internal control. OBJECTIVE: To develop a spinal implant animal infection model to simulate postoperative gram-negative wound infection. SUMMARY OF BACKGROUND DATA: Implant-associated surgical site infections (SSI) remain a dreaded complication of spinal surgery. Currently, over 30% of all spine SSIs are secondary to gram-negative bacteria. Traditional animal models have utilized gram-positive inoculums to simulate postoperative infection, but there exists no model in the literature for gram-negative infection in the setting of spinal instrumentation...
April 24, 2017: Spine
https://www.readbyqxmd.com/read/28440605/-carbapenem-resistance-in-pseudomonas-aeruginosa-isolated-from-urine-cultures-prevalence-and-risk-factors
#7
J Álvarez-Otero, J L Lamas-Ferreiro, L González-González, I Rodríguez-Conde, M J Fernández-Soneira, A Arca-Blanco, J R Bermúdez-Sanjurjo, J de la Fuente-Aguado
OBJECTIVE: Pseudomonas aeruginosa is a non-fermentative gram-negative bacillus with a great ability to develop resistance to multiple antibiotics, including carbapenems, which is a growing problem worldwide. The aim of this study was to analyse the prevalence of carbapenem-resistant P. aeruginosa (CRPA) in urine cultures and to determine the risk factors associated with the development of carbapanem resistance. METHODS: Positive urine cultures to P. aeruginosa between September 2012 and September 2014 were identified...
April 25, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28440203/antimicrobial-biofilm-inhibitory-and-anti-infective-activity-of-metallic-nanoparticles-against-multi-drug-resistant-mrsa-and-pseudomonas-aeruginosa-pa01
#8
Jamuna Bai Ashwathanarayan, Ravishankar Rai Vittal
BACKGROUND: The emergence of drug resistant pathogens is a major concern to the scientific community. Novel approaches such as the use of functionalized nanomaterials with antimicrobial activity is required to treat infectious diseases. OBJECTIVE: In the present study, the metallic nanoparticles (iron, gold, zinc oxide and copper oxide) were evaluated for the antimicrobial, biofilm inhibitory and anti-infective activity against human pathogens methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa PA01...
April 24, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28439680/microbiological-evaluation-of-infected-pelvic-lymphocele-after-robotic-prostatectomy-potential-predictors-for-culture-positivity-and-selection-of-the-best-empirical-antimicrobial-therapy
#9
Alaa Hamada, Catalina Hwang, Jorge Fleisher, Ingolf Tuerk
OBJECTIVES: To examine symptomatology and microbiology of infected lymphocele (LC) post-robotic-assisted radical prostatectomy and pelvic lymph node dissection (PLND) and to assess for potential predictors for LC fluid culture positivity. Secondly, to provide general recommendations about use of select antimicrobial therapy. METHODS: This was a single-center, IRB-approved, retrospective, case series review conducted between October 2008 and October 2014. Data included symptomatology, microbiology of symptomatic LC in men post-robotic prostatectomy and PLND...
April 24, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28439678/aquimarina-seongsanensis-sp-nov-isolated-from-sea-water
#10
Yoojin Oh, Bong-Geun Choi, Joon Yong Kim, Seong Woon Roh, Sung-Jae Lee
An orange, rod-shaped, gliding bacterium, designated strain CBA3208(T), was isolated from sea water of Jeju Island, Republic of Korea. Cells were observed to be Gram-stain negative, strictly aerobic, catalase positive and oxidase negative and to hydrolyse starch, gelatin, and Tweens 40 and 80. The major fatty acids were identified as iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The only isoprenoid quinone was found to be menaquinone-6 (MK-6) and the major polar lipids were identified as phosphatidylethanolamine, two aminolipids and four unidentified polar lipids...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439602/antibiogram-profile-of-septic-meningitis-among-children-in-duhok-iraq
#11
Abdulrahman T Saadi, Nadir A Garjees, Aveen H Rasool
  To determine the prevalence of bacteria in the cerebrospinal fluid (CSF), and the antibiogram profile in pediatric patients with suspected meningitis. Methods: This descriptive study was conducted between January 2014 and January 2016 in the Hevi Paediatric Teaching Hospital in Duhok, Iraq. The CSF samples were withdrawn from 432 pediatric patients suspected of meningitis. The samples were cultured, and antibiotic sensitivity tests were performed.  Results: There were 33 (7.6%) culture positive cases among 432 CSF samples...
May 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28439390/nosocomial-pseudomonas-putida-meningitis-a-case-report-and-literature-review
#12
Fahmi Yousef Khan, Mohammed AbuKamar, Deshmukh Anand
We report a case of Pseudomonas putida meningitis in a 26-year-old Nepalese man who was admitted to Hamad General Hospital with epidermoid cyst for drainage. Ommaya reservoir was placed into the cyst for drainage and externalventricular drainage (EVD) was performed. After four days, the patient was transferred to the ward in stable condition. His weakness resolved partially and headache severity decreased. After three days, the patient developed fever and headache severity increased with deterioration of consciousness level...
March 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/28439259/increased-biological-activity-of-aneurinibacillus-migulanus-strains-correlates-with-the-production-of-new-gramicidin-secondary-metabolites
#13
Faizah N Alenezi, Imen Rekik, Ali Chenari Bouket, Lenka Luptakova, Hedda J Weitz, Mostafa E Rateb, Marcel Jaspars, Stephen Woodward, Lassaad Belbahri
The soil-borne gram-positive bacteria Aneurinibacillus migulanus strain Nagano shows considerable potential as a biocontrol agent against plant diseases. In contrast, A. migulanus NCTC 7096 proved less effective for inhibition of plant pathogens. Nagano strain exerts biocontrol activity against some gram-positive and gram-negative bacteria, fungi and oomycetes through the production of gramicidin S (GS). Apart from the antibiotic effects, GS increases the rate of evaporation from the plant surface, reducing periods of surface wetness and thereby indirectly inhibiting spore germination...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28439041/cues-from-the-membrane-bacterial-glycerophospholipids
#14
Zachary D Dalebroux
In this issue of Journal of Bacteriology, Rowlett et al. unveil new Escherichia coli circuitry linking membrane glycerophospholipid (GPL) homeostasis to bacterial stress response and adaptation mechanisms. Glycerophospholipids comprise critical components of the dual-membrane envelope of Gram-negative bacteria and participate in many processes. The new evidence suggests that in some instances distinct E. coli GPL molecules function for distinct biochemistry, and bacteria sense perturbations in membrane GPL concentrations to coordinate survival strategies...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28439036/reclassification-of-the-specialized-metabolite-producer-pseudomonas-mesoacidophila-atcc-31433-as-a-member-of-the-burkholderia-cepacia-complex
#15
E Joel Loveridge, Cerith Jones, Matthew J Bull, Suzy C Moody, Małgorzata W Kahl, Zainab Khan, Louis Neilson, Marina Tomeva, Sarah E Adams, Andrew C Wood, Daniel Rodriguez-Martin, Ingrid Pinel, Julian Parkhill, Eshwar Mahenthiralingam, John Crosby
Pseudomonas mesoacidophila ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, which produces the monobactam isosulfazecin and the β-lactam potentiating bulgecins. To characterize the biosynthetic potential of P. mesoacidophila ATCC 31433 its complete genome was determined using single molecule real time DNA sequence analysis. The 7.8 Mb genome comprised four replicons: three chromosomal, each encoding ribosomal RNA, and one plasmid. Phylogenetic analysis demonstrated that P...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438946/potassium-iodide-potentiates-antimicrobial-photodynamic-inactivation-mediated-by-rose-bengal-in-vitro-and-in-vivo-studies
#16
Xiang Wen, Xiaoshen Zhang, Grzegorz Szewczyk, Ahmed El-Hussein, Ying-Ying Huang, Tadeusz Sarna, Michael R Hamblin
Rose Bengal (RB) is a halogenated xanthene dye that has been used to mediate antimicrobial photodynamic inactivation for several years. While highly active against Gram-positive bacteria, RB is largely inactive in killing Gram-negative bacteria. We have discovered that addition of the non-toxic salt potassium iodide (100mM) potentiates green light (540nm)-mediated killing by up to six extra logs with Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive methicillin resistant Staphylococcus aureus, and fungal yeast Candida albicans...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438944/genomic-insights-into-mcr-1-positive-plasmids-carried-by-the-colistin-resistant-escherichia-coli-from-the-inpatients
#17
Qingjing Wang, Jian Sun, Youfa Ding, Xing-Ping Li, Ya-Hong Liu, Youjun Feng
Colistin, a family of cationic polypeptide antibiotics, acts as a final resort against infections by the multidrug-resistant Gram-negative pathogens.….
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438890/short-ftsz-filaments-can-drive-asymmetric-cell-envelope-constriction-at-the-onset-of-bacterial-cytokinesis
#18
Qing Yao, Andrew I Jewett, Yi-Wei Chang, Catherine M Oikonomou, Morgan Beeby, Cristina V Iancu, Ariane Briegel, Debnath Ghosal, Grant J Jensen
FtsZ, the bacterial homologue of eukaryotic tubulin, plays a central role in cell division in nearly all bacteria and many archaea. It forms filaments under the cytoplasmic membrane at the division site where, together with other proteins it recruits, it drives peptidoglycan synthesis and constricts the cell. Despite extensive study, the arrangement of FtsZ filaments and their role in division continue to be debated. Here, we apply electron cryotomography to image the native structure of intact dividing cells and show that constriction in a variety of Gram-negative bacterial cells, including Proteus mirabilis and Caulobacter crescentus, initiates asymmetrically, accompanied by asymmetric peptidoglycan incorporation and short FtsZ-like filament formation...
April 24, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28438599/newly-identified-invertebrate-type-lysozyme-splys-i-in-mud-crab-scylla-paramamosain-exhibiting-muramidase-deficient-antimicrobial-activity
#19
Jian Zhou, Shu Zhao, Wen-Hong Fang, Jun-Fang Zhou, Jing-Xiao Zhang, Hongyu Ma, Jiang-Feng Lan, Xin-Cang Li
Lysozymes are widely distributed immune effectors exerting muramidase activity against the peptidoglycan of the bacterial cell wall to trigger cell lysis. However, some invertebrate-type (i-type) lysozymes deficient of muramidase activity still exhibit antimicrobial activity. To date, the mechanism underlying the antimicrobial effect of muramidase-deficient i-type lysozymes remains unclear. Accordingly, this study characterized a novel i-type lysozyme, Splys-i, in the mud crab Scylla paramamosain. Splys-i shared the highest identity with the Litopenaeus vannamei i-type lysozyme (Lvlys-i2, 54% identity) at the amino acid level...
April 21, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28438317/a-novel-chromatographic-approach-to-distinguish-gram-positive-from-gram-negative-bacteria-using-exogenous-volatile-organic-compound-metabolites
#20
Susana Ramírez-Guízar, Hannah Sykes, John D Perry, Edward C Schwalbe, Stephen P Stanforth, Ma Cristina I Perez-Perez, John R Dean
This paper utilized L-alanine aminopeptidase activity as a useful approach to distinguish between Gram-negative and Gram-positive bacteria. This was done using two enzyme substrates, specifically 2-amino-N-phenylpropanamide and 2-amino-N-(4-methylphenyl)propanamide which liberated the volatile compounds aniline and p-toluidine, respectively. Two complementary analytical techniques have been used to identify and quantify the VOCs, specifically static headspace multicapillary column gas chromatography ion mobility spectrometry (SHS-MCC-GC-IMS) and headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS)...
April 12, 2017: Journal of Chromatography. A
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