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https://www.readbyqxmd.com/read/28727761/genome-wide-identification-of-the-potato-wrky-transcription-factor-family
#1
Chao Zhang, Dongdong Wang, Chenghui Yang, Nana Kong, Zheng Shi, Peng Zhao, Yunyou Nan, Tengkun Nie, Ruoqiu Wang, Haoli Ma, Qin Chen
WRKY transcription factors play pivotal roles in regulation of stress responses. This study identified 79 WRKY genes in potato (Solanum tuberosum). Based on multiple sequence alignment and phylogenetic relationships, WRKY genes were classified into three major groups. The majority of WRKY genes belonged to Group II (52 StWRKYs), Group III had 14 and Group I consisted of 13. The phylogenetic tree further classified Group II into five sub-groups. All StWRKY genes except StWRKY79 were mapped on potato chromosomes, with eight tandem duplication gene pairs and seven segmental duplication gene pairs found from StWRKY family genes...
2017: PloS One
https://www.readbyqxmd.com/read/28726522/steerable-induction-of-the-thymosin-%C3%A3-4-mrtf-a-pathway-via-aav-based-overexpression-induces-therapeutic-neovascularization
#2
Tilman Ziegler, Markus Kraus, Wira Husada, Florian Gesenhues, Qui Jiang, Olaf Pinkenburg, Teresa Trenkwalder, Karl-Ludwig Laugwitz, Ferdinand le Noble, Christian Weber, Christian Kupatt, Rabea Hinkel
Viral vectors have been frequently used in a variety of preclinical animal models to deliver genetic constructs into tissues. Among the vectors used, adeno-associated viral vectors (AAVs) may be targeted to specific tissues, depending on the serotype used. Moreover, they show robust expression for prolonged periods of time and have a low immunogenic potential. Furthermore, AAVs, unlike other vector systems, only display a low rate of genomic integration. However, to ensure efficient transgene production, expression is typically driven by constitutively active promoters, such as the CMV promotor...
July 20, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28716280/association-of-polymorphisms-and-reduced-expression-levels-of-the-nr4a2-gene-with-parkinson-s-disease-in-a-mexican-population
#3
Elizabeth Ruiz-Sánchez, Petra Yescas, Mayela Rodríguez-Violante, Nancy Martínez-Rodríguez, Jesica N Díaz-López, Adriana Ochoa, Sergio S Valdes-Rojas, Daniel Magos-Rodríguez, Julio C Rojas-Castañeda, Amin Cervantes-Arriaga, Samuel Canizales-Quinteros, Patricia Rojas
INTRODUCTION: The NR4A2 transcription factor is important in the development, survival and phenotype of dopaminergic neurons and it is postulated as a possible biomarker for Parkinson's disease (PD). Therefore, our aim was to analyze in a sample of a Mexican population with idiopathic PD, mutations (in two hotspot mutation regions) and two polymorphisms (rs34884856 in promotor and rs35479735 intronic regions) of the NR4A2 gene. We also evaluate the levels of NR4A2 gene expression in peripheral blood for a Mexican population, and identify whether they are associated with NR4A2 gene polymorphisms...
August 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28715805/an-alternative-promoter-in-intron1-of-the-renin-gene-is-regulated-by-glucose-starvation-via-serum-response-factor
#4
Philipp Lutze, Heike Wanka, Inga Bäumgen, Doreen Staar, Bianka Grunow, Jörg Peters
BACKGROUND/AIMS: Renin is known as a secretory glycoprotein that ultimately leads to angiotensin II generation. In this way renin exerts pro-inflammatory effects and promotes cardiac injury. Additional transcripts have been identified encoding for a cytosolic renin isoform that - in contrast to secretory renin - exhibits cardioprotective effects under ischemic conditions. The promoter of these transcripts is unknown. METHODS: Using qRT-PCR and dual-luciferase reporter assay we examined the expression and promotor activity of cytosolic renin as well as the regulation by glucose starvation in H9c2 cardiomyoblasts...
July 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28714542/two-enabling-strategies-for-the-stereoselective-conversion-of-internal-alkynes-into-trisubstituted-alkenes
#5
Alois Fürstner, Nikolas Huwyler, Karin Radkowski, Stephan M Rummelt
An expedient method for the C-methylation of alkenylstannanes with formation of trisubstituted alkenes is described, which relies on the use of MeI in combination with copper thiophene-2-carboxylate (CuTC) as promotor and tetra-n-butylammonium diphenylphosphinate as an effective tin scavenger; in some cases, it proved beneficial to further supplement the mixture with catalytic amounts of Pd(PPh3)4. Under these conditions, the reaction proved robust, high yielding and compatible with many functional groups that might not subsist under more traditional conditions used to C-alkylate organotin derivatives...
July 17, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28711285/anti-il-21-monoclonal-antibody-combined-with-liraglutide-effectively-reverses-established-hyperglycemia-in-mouse-models-of-type-1-diabetes
#6
Anna K Rydén, Nikole R Perdue, Philippe P Pagni, Claire B Gibson, Sowbarnika S Ratliff, Rikke K Kirk, Travis J Friesen, Claus Haase, Ken Coppieters, Matthias G von Herrath, Tamar E Boursalian
Immunotherapy for type 1 diabetes (T1D) has previously focused on suppressing the autoimmune response against pancreatic beta cells to preserve endogenous insulin production and regulate glucose levels. With increased attention toward combination therapy strategies, studies indicate the multifunctional cytokine interleukin-21 (IL-21) may be a suitable target as an immuno-modulatory arm, while glucagon-like peptide-1 receptor (GLP-1R) agonists may be appropriate as a beta cell protective arm in combination therapy for T1D...
July 12, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28708324/ferrichrome-has-found-its-match-biomimetic-analogs-with-diversified-activity-map-discrete-microbial-targets
#7
Jenny Besserglick, Evgenia Olshvang, Agnieszka Szebesczyk, Joseph Englander, Dana Levinson, Yitzhak Hadar, Elzbieta Gumienna-Kontecka, Abraham Shanzer
Siderophores provide an established platform for studying molecular recognition principles in biological systems. Herein we describe the preparation of ferrichrome biomimetic analogs varying in length and polarity of the amino acid chain separating between the tripodal scaffold and the pendent FeIII chelating hydroxamic acid groups. Spectroscopic and potentiometric titrations determined their iron affinity to be within the range of efficient chelators. Microbial growth promotion and iron uptake studies were conducted on E...
July 14, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28706134/heparin-antagonizes-cisplatin-resistance-of-a2780-ovarian-cancer-cells-by-affecting-the-wnt-signaling-pathway
#8
Daniel Bastian Pfankuchen, Fabian Baltes, Tahira Batool, Jin-Ping Li, Martin Schlesinger, Gerd Bendas
Low molecular weight heparin (LMWH), the guideline based drug for prophylaxis and treatment of cancer-associated thrombosis, was recently shown to sensitize cisplatin resistant A2780cis human ovarian cancer cells for cisplatin cytotoxicity upon 24 h pretreatment with 50 μg × mL-1 of the LMWH tinzaparin in vitro, equivalent to a therapeutic dosage. Thereby, LMWH induced sensitization by transcriptional reprogramming of A2780cis cells via not yet elucidated mechanisms that depend on cellular proteoglycans. Here we aim to illuminate the underlying molecular mechanisms of LMWH in sensitizing A2780cis cells for cisplatin...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28701913/common-hepatic-branch-of-vagus-nerve-dependent-expression-of-immediate-early-genes-in-the-mouse-brain-by-intraportal-l-arginine-comparison-with-cholecystokinin-8
#9
Daisuke Yamada, Peter Koppensteiner, Saori Odagiri, Megumi Eguchi, Shun Yamaguchi, Tetsuya Yamada, Hideki Katagiri, Keiji Wada, Masayuki Sekiguchi
Information from the peripheral organs is thought to be transmitted to the brain by humoral factors and neurons such as afferent vagal or spinal nerves. The common hepatic branch of the vagus (CHBV) is one of the main vagus nerve branches, and consists of heterogeneous neuronal fibers that innervate multiple peripheral organs such as the bile duct, portal vein, paraganglia, and gastroduodenal tract. Although, previous studies suggested that the CHBV has a pivotal role in transmitting information on the status of the liver to the brain, the details of its central projections remain unknown...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28688997/maternal-body-mass-index-impacts-fetal-placental-size-at-birth-and-umbilical-cord-oxygen-values-with-implications-for-regulatory-mechanisms
#10
Bryan S Richardson, Stephanie Ruttinger, Hilary K Brown, Timothy R H Regnault, Barbra de Vrijer
BACKGROUND: Maternal under- and over-nutrition are known to effect fetal growth with altered placental development and nutrient transport, but whether fetal oxygenation is also altered remains unknown. AIMS: To examine linkages between maternal BMI and birth weights, placental weights, and umbilical vein and artery PO2, with implications for signaling mechanisms. STUDY DESIGN: Population-based cohort study. SUBJECTS: Analysis of hospital database information on all patients with pre-pregnant BMI values delivering viable, singleton infants between Jan 1, 1999 and Dec 31, 2010 (N=29,212)...
July 6, 2017: Early Human Development
https://www.readbyqxmd.com/read/28682619/structure-reactivity-relationships-of-conformationally-armed-disaccharide-donors-and-their-use-in-the-synthesis-of-a-hexasaccharide-related-to-the-capsular-polysaccharide-from-streptococcus-pneumoniae-type-37
#11
Thibault Angles d'Ortoli, Christoffer Hamark, Göran Widmalm
To advance the field of glycobiology, efficient synthesis methods of oligosaccharides and glycoconjugates are a requisite. In glycosylation reactions using superarmed donors, both selectivity and reactivity issues must be considered, and we herein investigate these aspects for differently protected β-linked 2-O-glycosylated glucosyl donors carrying bulky tert-butyldimethylsilyl groups to different extents. The acceptors in reactions being secondary alcohols presents a challenging situation with respect to steric crowding...
July 19, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28672704/control-of-disinfection-byproducts-dbps-by-ozonation-and-peroxone-process-role-of-chloride-on-removal-of-dbp-precursors
#12
Popta Deeudomwongsa, Songkeart Phattarapattamawong, Kun-Yi Andrew Lin
The objective of this study was to remove regulated DBP precursors by using ozonation and peroxone process (H2O2/O3). Regarding formation potentials of trihalomethanes (THMs) and haloacetic acids (HAAs), the role of chloride in chlorination and ozonation/peroxone processes was revealed. The organic compounds in water samples from rapid sand filtration preferably yielded the THM formation potentials, rather than HAAs. Ozonation with the typical applied doses (1-5 mg L(-1)) was ineffective for removals of THM and HAA precursors...
June 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/28668496/generation-of-nanobodies-against-slyd-and-development-of-tools-to-eliminate-this-bacterial-contaminant-from-recombinant-proteins
#13
Yaozhong Hu, Ema Romão, Didier Vertommen, Cécile Vincke, Francisco Morales-Yánez, Carlos Gutiérrez, Changxiao Liu, Serge Muyldermans
The gene for a protein domain, derived from a tumor marker, fused to His tag codons and under control of a T7 promotor was expressed in E. coli strain BL21 (DE3). The recombinant protein was purified from cell lysates through immobilized metal affinity chromatography and size-exclusion chromatography. A contaminating bacterial protein was consistently co-purified, even using stringent washing solutions containing 50 or 100 mM imidazole. Immunization of a dromedary with this contaminated protein preparation, and the subsequent generation and panning of the immune Nanobody library yielded several Nanobodies of which 2/3 were directed against the bacterial contaminant, reflecting the immunodominance of this protein to steer the dromedary immune response...
June 28, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28657145/macrophage-migration-inhibitory-factor-mif-gene-promotor-polymorphism-is-associated-with-increased-fibrosis-in-biliary-atresia-patients-but-not-with-disease-susceptibility
#14
Khaled H Sadek, Sameera Ezzat, Samira A Abdel-Aziz, Hanaa Alaraby, Asmaa Mosbeh, Mohamed H Abdel-Rahman
Two polymorphisms, rs755622 and rs5844572, in the promoter region of the macrophage migration inhibitory factor (MIF) gene influence the basal and/or induced transcriptional activity and have been linked to several inflammatory and autoimmune diseases. The aim of this study was to investigate the association between these two polymorphisms and disease susceptibility in patients with biliary atresia (BA). Allele frequencies of rs755622 and rs5844572 were assessed in 60 Egyptian infants with a confirmed diagnosis of BA...
June 28, 2017: Annals of Human Genetics
https://www.readbyqxmd.com/read/28655335/identification-of-g-quadruplex-structures-that-possess-transcriptional-regulating-functions-in-the-dele-and-cdc6-cpg-islands
#15
Daniyah H Bay, Annika Busch, Fred Lisdat, Keisuke Iida, Kazunori Ikebukuro, Kazuo Nagasawa, Isao Karube, Wataru Yoshida
BACKGROUND: G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands DNA microarray with a fluorescent-labeled G-quadruplex ligand. Among these putative G-quadruplex-forming sequences, G-quadruplex formation was verified for 10 randomly selected sequences by CD spectroscopy and DMS footprinting analysis. In this study, the biological function of the 10 G-quadruplex-forming sequences in the transcriptional regulation has been analyzed using a reporter assay...
June 27, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28648603/effects-of-brn2-overexpression-on-eccrine-sweat-gland-development-in-the-mouse-paw
#16
Min Keun Chee, Seong Kyeong Jo, Kyung Cheol Sohn, Chang Deok Kim, Jeung-Hoon Lee, Young Ho Lee
Eccrine sweat glands regulate body temperature by secreting water and electrolytes. In humans, eccrine sweat glands are ubiquitous in the skin, except in the lips and external genitalia. In mice, eccrine sweat glands are present only in the paw pad. Brn2 is a protein belonging to a large family of transcription factors. A few studies have examined Brn2 in melanoma cells and epidermal keratinocytes. This study investigated changes in the skin in the K5-Brn2 transgenic mouse, which overexpresses Brn2 and contains the keratin 5 promotor...
June 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28639280/mutational-profiles-of-brenner-tumors-show-distinctive-features-uncoupling-urothelial-carcinomas-and-ovarian-carcinoma-with-transitional-cell-histology
#17
Nicole Pfarr, Silvia Darb-Esfahani, Jonas Leichsenring, Eliane Taube, Melanie Boxberg, Ioana Braicu, Moritz Jesinghaus, Roland Penzel, Volker Endris, Aurelia Noske, Wilko Weichert, Peter Schirmacher, Carsten Denkert, Albrecht Stenzinger
Brenner tumors (BT) are rare ovarian tumors encompassing benign, borderline and malignant variants. While the histopathology of BTs and their clinical course is well described, little is known about the underlying genetic defects. We employed targeted next generation sequencing to analyze the mutational landscape in a cohort of 23 BT cases (17 benign, 2 borderline, 4 malignant) and 3 ovarian carcinomas with transitional cell histology (TCC). Copy number variations (CNV) were validated by fluorescence in-situ hybridization (FISH) and quantitative PCR-based copy number assays...
June 22, 2017: Genes, Chromosomes & Cancer
https://www.readbyqxmd.com/read/28638786/polyelectrolyte-enrobed-cancer-cells-in-view-of-personalized-immune-therapy
#18
Lien Lybaert, Keun Ah Ryu, Riet De Rycke, Alfred C Chon, Olivier De Wever, Karim Y Vermaelen, Aaron Esser-Kahn, Bruno G De Geest
Targeting the immune system with a personalized vaccine containing cues derived from the patient's malignancy might be a promising approach in the fight against cancer. It includes neo-antigens as well as nonmutated tumor antigens, preferentially leading to an immune response that is directed to a broader range of epitopes compared to strategies involving a single antigen. Here, this paper reports on an elegant method to encapsulate whole cancer cells into polyelectrolyte particles. Porous and nonaggregated microparticles containing dead cancer cells are obtained by admixing mannitol and live cancer cells with oppositely charged polyelectrolytes, dextran sulfate (anionic polysaccharide), and poly-l-arginine (cationic polypeptide) prior to atomization into a hot air stream...
June 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28638109/voxel-based-morphometric-brain-comparison-between-healthy-subjects-and-major-depressive-disorder-patients-in-japanese-with-the-s-s-genotype-of-5-httlpr
#19
Natsuki Igata, Shingo Kakeda, Keita Watanabe, Satoru Ide, Taro Kishi, Osamu Abe, Ryouhei Igata, Asuka Katsuki, Nakao Iwata, Reiji Yoshimura, Yukunori Korogi
Individuals with s/s genotype of serotonin transporter gene-linked promotor region (5-HTTLPR), which appear with a high frequency in Japanese, exhibit more diagnosable depression in relation to stressful life events than those with the s/l or l/l genotype. We prospectively investigated the brain volume changes in first-episode and medication naïve major depression disorder patients (MDD) with the s/s genotype in Japanese. We assessed the differences between 27 MDD with the s/s genotype and 44 healthy subjects (HS) with the same genotype using a whole-brain voxel-by-voxel statistical analysis of MRI...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28627215/la-red-de-promotores-de-salud-como-estrategia-de-educaci%C3%A3-n-alimentaria-el-ejemplo-del-programa-edalnu-1963-1994
#20
María Tormo Santamaría, Eva María Trescastro López, Pamela Pereyra Zamora, María Eugenia Galiana Sánchez, Josep Bernabeu-Mestre
La educación en alimentación y nutrición es una herramienta fundamental para garantizar la salud. En 1961, se puso en marcha el Programa de Educación en Alimentación y Nutrición (EDALNU), que ayudó a la población española a completar su transición alimentaria y nutricional. El objetivo de este trabajo es analizar las características de la red de promotores de salud que se desarrolló en el marco del programa.Recibieron formación relacionada con alimentación y nutrición 46.752 personas, el 94% de ellas mujeres...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
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