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https://www.readbyqxmd.com/read/28922831/the-transcription-factors-coup-tfi-and-coup-tfii-have-distinct-roles-in-arealisation-and-gabaergic-interneuron-specification-in-the-early-human-fetal-telencephalon
#1
Ayman Alzu'bi, Susan J Lindsay, Lauren F Harkin, Jack McIntyre, Steven N Lisgo, Gavin J Clowry
In human telencephalon at 8-12 postconceptional weeks, ribonucleic acid quantitative sequencing and immunohistochemistry revealed cortical chicken ovalbumin upstream promotor-transcription factor 1 (COUP-TFI) expression in a high ventro-posterior to low anterior gradient except for raised immunoreactivity in the anterior ventral pallium. Unlike in mouse, COUP-TFI and SP8 were extensively co-expressed in dorsal sensory neocortex and dorsal hippocampus whereas COUPTFI/COUPTFII co-expression defined ventral temporal cortex and ventral hippocampus...
October 1, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28903770/a-novel-bi-directional-promoter-system-allows-tunable-recombinant-protein-production-in-pichia-pastoris
#2
Vignesh Rajamanickam, Karl Metzger, Christian Schmid, Oliver Spadiut
BACKGROUND: The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Both promoter systems have several advantages and disadvantages; with one of the main disadvantages being their lack of tunability. Various novel promoter systems, which are either inducible or de-repressed, allowing higher degrees of freedom, have been reported...
September 13, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28894890/new-prognostic-factor-telomerase-reverse-transcriptase-promotor-mutation-presents-without-mr-imaging-biomarkers-in-primary-glioblastoma
#3
Tunc F Ersoy, Vera C Keil, Dariusch R Hadizadeh, Gerrit H Gielen, Rolf Fimmers, Andreas Waha, Barbara Heidenreich, Rajiv Kumar, Hans H Schild, Matthias Simon
PURPOSE: Magnetic resonance (MR) imaging biomarkers can assist in the non-invasive assessment of the genetic status in glioblastomas (GBMs). Telomerase reverse transcriptase (TERT) promoter mutations are associated with a negative prognosis. This study was performed to identify MR imaging biomarkers to forecast the TERT mutation status. METHODS: Pre-operative MRIs of 64/67 genetically confirmed primary GBM patients (51/67 TERT-mutated with rs2853669 polymorphism) were analyzed according to Visually AcceSAble Rembrandt Images (VASARI) ( https://wiki...
September 11, 2017: Neuroradiology
https://www.readbyqxmd.com/read/28888782/tnfaip3-levels-in-lung-dendritic-cells-instruct-th2-or-th17-cell-differentiation-in-eosinophilic-or-neutrophilic-asthma
#4
Heleen Vroman, Ingird M Bergen, Jennifer A C van Hulst, Memno van Nimwegen, Denise van Uden, Martin J Schuijs, Saravanan Y Pillai, Geert van Loo, Hamida Hammad, Bart N Lambrecht, Rudi W Hendriks, Mirjam Kool
BACKGROUND: It is currently unknown, why allergen exposure or environmental triggers in mild to moderate asthma patients results in Th2-mediated eosinophilic inflammation, whereas severe asthma patients often present with Th17-mediated neutrophilic inflammation. The activation state of dendritic cells (DCs) is crucial for both Th2 and Th17-cell differentiation, and is mediated through NF-κB activation. Ablation of TNFAIP3, one of the crucial negative regulators of NF-κB activation in myeloid cells and DCs was shown to control DC activation...
September 6, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28886314/alterations-on-high-hbf-levels-may-be-associated-with-klf1-gene-mutations
#5
M Aydin, E Rencuzogullari, S Bayram, Y Sevgiler, A Genc
The KLF1 gene synthesizes a transcription factor in the zinc finger structure that regulates the transcription of β-, γ-globin, and Foxm1 genes. This factor plays an important role in the erythropoiesis mechanism by modifying the chromatin structure and is involved in the regulation of transcription in the opening of the β-globin gene. β-globin gene expression could be disrupted by a mutation, which may be a possible cause of a disruption in regulation of the promotor of the β-globin gene where the KLF1 transcription factor binds...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28881635/htlv-1-tax-upregulates-early-growth-response-protein-1-through-nuclear-factor-%C3%AE%C2%BAb-signaling
#6
Qingsong Huang, Zhiguo Niu, Jingxian Han, Xihong Liu, Zhuangwei Lv, Huanhuan Li, Lixiang Yuan, Xiangping Li, Shuming Sun, Hui Wang, Xinxiang Huang
Human T cell leukemia virus type 1 (HTLV-1) is a complex retrovirus that causes adult T cell leukemia (ATL) in susceptible individuals. The HTLV-1-encoded oncoprotein Tax induces persistent activation of the nuclear factor-κB (NF-κB) pathway. Early growth response protein 1 (EGR1) is overexpressed in HTLV-1-infected T cell lines and ATL cells. Here, we showed that both Tax expression and HTLV-1 infection promoted EGR1 overexpression. Loss of the NF-κB binding site in the EGR1 promotor or inhibition of NF-κB activation reduced Tax-induced EGR1 upregulation...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28867247/yin-yang-1-suppresses-pancreatic-ductal-adenocarcinoma-cell-proliferation-and-tumor-growth-by-regulating-sox2ot-sox2-axis
#7
Jing-Jing Zhang, Yi Zhu, Xiong-Fei Zhang, Dong-Fang Liu, Yan Wang, Chuang Yang, Guo-Dong Shi, Yun-Peng Peng, Kai Zhang, Lei Tian, Yi Miao, Kui-Rong Jiang
The transcription regulator Yin Yang-1 (YY1) serves as a tumor suppressor in pancreatic ductal adenocarcinoma (PDAC). However, the function of YY1 in proliferation of PDAC cells remains to be clarified. In this study, we found that overexpression of YY1 suppressed proliferation and decreased the expression of long non-coding RNA (lncRNA) SOX2OT and its potential target gene SOX2 in PDAC cells. Luciferase reporter, electrophoretic mobility shift (EMSA), and chromatin immunoprecipitation (ChIP) assays revealed binding of YY1 to the SOX2OT promoter...
September 1, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28856689/real-time-large-scale-in-vivo-observations-reveal-intrinsic-synchrony-plasticity-and-growth-cone-dynamics-of-midline-crossing-axons-during-neuronal-wiring-of-the-zebrafish-spinal-cord
#8
Søren S L Andersen
How axons are wired in the vertebrate spinal cord has been studied mostly using fixed samples or looking at individually growing axons. Using light-sheet microscopy, early neural development is here followed in vivo in real time at high resolution along several hundred micrometers of the zebrafish spinal cord. The dynamics and time course of cellular development and axonal wiring of interneurons expressing GFP under control of the dmrt3 promotor are analyzed. Following neurulation, commissural axons are observed crossing the ventral floor plate midline perpendicularly at about 20 microns/h and in a manner dependent on the Robo3 but not the EphA4 receptors...
August 30, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28853394/tumor-promoting-function-of-apoptotic-caspases-by-an-amplification-loop-involving-ros-macrophages-and-jnk-in-drosophila
#9
Andreas Bergmann, Ernesto Perez, Jillian L Lindblad
Apoptosis and its molecular mediators, the caspases, have long been regarded as tumor suppressors and one hallmark of cancer is "Evading Apoptosis". However, recent work has suggested that apoptotic caspases can also promote proliferation and tumor growth under certain conditions. How caspases promote proliferation and how cells are protected from the potentially harmful action of apoptotic caspases is largely unknown. Here, we show that although caspases are activated in a well-studied neoplastic tumor model in Drosophila, oncogenic mutations of the proto-oncogene Ras (Ras(V12)) maintain tumorous cells in an "undead"-like condition and transform caspases from tumor suppressors into tumor promotors...
August 30, 2017: ELife
https://www.readbyqxmd.com/read/28851281/growth-phase-dependent-expression-profiles-of-three-vital-h-ns-family-proteins-encoded-on-the-chromosome-of-pseudomonas-putida-kt2440-and-on-the-pcar1-plasmid
#10
Zongping Sun, Delyana Vasileva, Chiho Suzuki-Minakuchi, Kazunori Okada, Feng Luo, Yasuo Igarashi, Hideaki Nojiri
BACKGROUND: H-NS family proteins are nucleoid-associated proteins that form oligomers on DNA and function as global regulators. They are found in both bacterial chromosomes and plasmids, and were suggested to be candidate effectors of the interaction between them. TurA and TurB are the predominantly expressed H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440, while Pmr is encoded on the carbazole-degradative incompatibility group P-7 plasmid pCAR1. Previous transcriptome analyses suggested that they function cooperatively, but play different roles in the global transcriptional network...
August 29, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28837843/positive-influence-of-partial-resection-on-overall-survival-of-patients-with-overlapping-glioblastomas
#11
Johanna Quick-Weller, Stephanie Tritt, Peter Baumgarten, Jürgen Konczalla, Sepide Kashefiolasl, Anika Noack, Julia Tichy, Volker Seifert, Gerhard Marquardt
OBJECTIVES: Patients with overlapping glioblastomas (former known as gliomatosis cerebri according to the 2007 WHO classification) have a poor prognosis. Most of the patients undergo biopsy to confirm histopathological diagnosis. Treatment comprises chemotherapy, radiation and combination of both. We determined whether resection of the contrast enhancing tumor parts leads to a prolonged survival. PATIENTS AND METHODS: We performed a retrospective analysis and included 31 patients with overlapping glioblastomas (OG) who showed WHO IV in the initial histopathological examination...
June 29, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28817493/dna-methylation-of-the-glucocorticoid-receptor-gene-promoter-in-the-placenta-is-associated-with-blood-pressure-regulation-in-human-pregnancy
#12
Sulistyo E Dwi Putra, Christoph Reichetzeder, Martin Meixner, Karsten Liere, Torsten Slowinski, Berthold Hocher
BACKGROUND: Blood pressure (BP) regulation during pregnancy is influenced by hormones of placental origin. It was shown that the glucocorticoid system is altered in hypertensive pregnancy disorders such as preeclampsia. Epigenetic mechanism might influence the activity of genes involved in placental hormone/hormone receptor synthesis/action during pregnancy. METHOD: In the current study, we analyzed the association of 5'-C-phosphate-G-3' (CpG) site methylation of different glucocorticoid receptor gene (NR3C1) promoter regions with BP during pregnancy...
August 16, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28801786/the-grapevine-vvcax3-is-a-cation-h-exchanger-involved-in-vacuolar-ca-2-homeostasis
#13
Viviana Martins, Filipa Carneiro, Carlos Conde, Mariana Sottomayor, Hernâni Gerós
The grapevine VvCAX3 mediates calcium transport in the vacuole and is mostly expressed in green grape berries and upregulated by Ca (2+) , Na (+) and methyl jasmonate. Calcium is an essential plant nutrient with important regulatory and structural roles in the berries of grapevine (Vitis vinifera L.). On the other hand, the proton-cation exchanger CAX proteins have been shown to impact Ca(2+) homeostasis with important consequences for fruit integrity and resistance to biotic/abiotic stress. Here, the CAX gene found in transcriptomic databases as having one of the highest expressions in grapevine tissues, VvCAX3, was cloned and functionally characterized...
August 11, 2017: Planta
https://www.readbyqxmd.com/read/28792376/construction-of-an-inactivated-typhoid-vaccine-candidate-expressing-escherichia-coli-heat-labile-enterotoxin-b-subunit-and-evaluation-of-its-immunogenicity-in-a-murine-model
#14
Boram Kim, Gayeon Won, John Hwa Lee
PURPOSE: Typhoid fever caused by Salmonella enterica serovar Typhi has contributed to the global public health burden, particularly in developing countries. In this study, an S. Typhi ghost was developed and its capacity as a vaccine candidate against typhoid fever was assessed. METHODOLOGY: An asd+ plasmid pJHL187 harbouring a ghost cassette comprising the PhiX 174 Elysis gene tightly controlled under the convergent promotor system was transformed into an asd gene-deleted mutant S...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28791452/long-term-daily-temozolomide-with-dose-dependent-efficacy-in-mgmt-promotor-methylation-negative-recurrent-high-grade-astrocytoma
#15
Zhengqiu Zhou, Tracy A Howard, John L Villano
Temozolomide (TMZ) for malignant gliomas is traditionally dosed in 5 out of a 28-day cycle, however alternative regimens exist, including dose-dense. Continuous daily dosing is available, but the acceptable dose and duration of therapy is unknown. We document a 40-year-old male with recurrent anaplastic astrocytoma, IDH mutant and MGMT promotor methylation negative, who has well-tolerated continuous daily TMZ for 20 months at 100 mg per day for nearly the length of this period. A trial at 80 mg per day demonstrated disease progression with response upon return to 100 mg per day...
August 8, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28777432/comparison-of-a-prototype-reverse-hybridization-assay-and-methylight-for-detection-of-sfrp2-promotor-methylation-in-fecal-dna
#16
Gernot Kriegshäuser, Dietmar Enko, Matthias Zitt, Michael Oberwalder, Christian Oberkanins, Dietmar Öfner, Robert Zeillinger, Hannes Maximilian Müller
BACKGROUND: This study aimed to evaluate the diagnostic performance of a novel nonquantitative methylation-specific reverse hybridization (MSRH) assay to detect secreted frizzled-related protein 2 (SFRP2) promotor methylation in fecal DNA. METHODS: SFRP2 promoter methylation was investigated in stool DNA isolated from 18 colorectal cancer (CRC) patients and 22 healthy controls using the MSRH assay based on methylation-specific DNA amplification followed by reverse hybridization of biotinylated amplicons to sequence-specific methylation detection probes, with MethyLight serving as a reference method...
July 26, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28776955/antineoplastic-effects-of-histone-deacetylase-inhibitors-in-neuroendocrine-cancer-cells-are-mediated-through-transcriptional-regulation-of-notch1-by-activator-protein-1
#17
Samuel Jang, Haining Jin, Madhuchhanda Roy, Alice L Ma, Shaoqin Gong, Renata Jaskula-Sztul, Herbert Chen
Notch signaling is minimally active in neuroendocrine (NE) cancer cells. While histone deacetylase inhibitors (HDACi) suppress NE cancer growth by inducing Notch, the molecular mechanism underlying this interplay has not yet been defined. NE cancer cell lines BON, H727, and MZ-CRC-1 were treated with known HDACi Thailadepsin-A (TDP-A) and valproic acid (VPA), and Notch1 mRNA expression was measured with RT-PCR. Truncated genomic fragments of the Notch1 promotor region fused with luciferase reporter were used to identify the potential transcription factor (TF) binding site...
August 4, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28774282/interaction-between-nfatc2-and-the-transcription-factor-sp1-in-pancreatic-carcinoma-cells-patu-8988t
#18
Manuela Malsy, Bernhard Graf, Katrin Almstedt
BACKGROUND: Nuclear factors of activated T-cells (NFATs) have been mainly characterized in the context of immune response regulation because, as transcription factors, they have the ability to induce gene transcription. NFAT proteins are found in several types of tumors, for instance, pancreatic carcinoma. The role of NFATs in carcinogenesis is regulating central genes in cell differentiation and cell growth. NFAT proteins are primarily located in cytoplasm and only transported to the cell nucleus after activation...
August 3, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28768829/the-saga-complex-together-with-transcription-factors-and-the-endocytic-protein-rvs167p-coordinate-the-reprofiling-of-gene-expression-in-response-to-changes-in-sterol-composition-in-saccharomyces-cerevisiae
#19
Gisèle Dewhurst-Maridor, Daniel Abegg, Fabrice P A David, Jacques Rougemont, Cameron C Scott, Alexander Adibekian, Howard Riezman
Changes in cellular sterol species and concentrations can have profound effects on the transcriptional profile. In yeast, mutants defective in sterol biosynthesis show a wide range of changes in transcription, including a co-induction of anaerobic genes and ergosterol biosynthesis genes, biosynthesis of basic amino acids, and several stress genes. However the mechanisms underlying these changes are unknown. We identified mutations in the SAGA complex, a co-activator of transcription, which abrogate the ability to carry out most of these sterol-dependent transcriptional changes...
August 2, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28755557/amphiphilic-gemini-pyridinium-mediated-incorporation-of-zn-ii-meso-tetrakis-4-carboxyphenyl-porphyrin-into-water-soluble-gold-nanoparticles-for-photodynamic-therapy
#20
María E Alea-Reyes, Jorge Soriano, Inma Mora-Espí, Mafalda Rodrigues, David A Russell, Leonardo Barrios, Lluïsa Pérez-García
Zn-containing porphyrins are intensely investigated for their ability to form reactive oxygen species and thereby being potent photosensitizers for use in photodynamic therapy (PDT). Some of the drawbacks of the PDT approach, such as unspecific distribution, could be addressed by means of photosensitizer drug delivery systems. In this work, we synthesize and characterize new water-soluble gold nanoparticles (GNP) stabilized by a mixture of a polyethyleneglycol-containing thiol (to improve water solubility) and a new amphiphilic gemini-type pyridinium salt, which also acts as promotor of the incorporation of the anionic photosensitizer Na-ZnTCPP into the GNP...
July 18, 2017: Colloids and Surfaces. B, Biointerfaces
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