keyword
MENU ▼
Read by QxMD icon Read
search

Promotor

keyword
https://www.readbyqxmd.com/read/28422998/reducing-dna-context-dependence-in-bacterial-promoters
#1
Swati B Carr, Jacob Beal, Douglas M Densmore
Variation in the DNA sequence upstream of bacterial promoters is known to affect the expression levels of the products they regulate, sometimes dramatically. While neutral synthetic insulator sequences have been found to buffer promoters from upstream DNA context, there are no established methods for designing effective insulator sequences with predictable effects on expression levels. We address this problem with Degenerate Insulation Screening (DIS), a novel method based on a randomized 36-nucleotide insulator library and a simple, high-throughput, flow-cytometry-based screen that randomly samples from a library of 436 potential insulated promoters...
2017: PloS One
https://www.readbyqxmd.com/read/28418873/combined-inhibition-of-gli-and-flt3-signaling-leads-to-effective-anti-leukemic-effects-in-human-acute-myeloid-leukemia
#2
Emily-Marie Latuske, Hauke Stamm, Marianne Klokow, Gabi Vohwinkel, Jana Muschhammer, Carsten Bokemeyer, Manfred Jücker, Maxim Kebenko, Walter Fiedler, Jasmin Wellbrock
Activation of the Hedgehog pathway has been implicated in the pathogenesis of several tumor types including myeloid leukemia. Previously we demonstrated that overexpression of Hedgehog downstream mediators GLI1/2 confers an adverse prognosis to patients with acute myeloid leukemia (AML) and is correlated with a FLT3 mutated status. To analyze a possible non-canonical activation of the Hedgehog pathway via FLT3 and PI3K, we performed blocking experiments utilizing inhibitors for FLT3 (sunitinib), PI3K (PF-04691502) and GLI1/2 (GANT61) in FLT3-mutated and FLT3 wildtype AML cell lines and primary blasts...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414318/initial-sites-of-hepadnavirus-integration-into-host-genome-in-human-hepatocytes-and-in-the-woodchuck-model-of-hepatitis-b-associated-hepatocellular-carcinoma
#3
R Chauhan, N D Churchill, P M Mulrooney-Cousins, T I Michalak
Hepatitis B virus (HBV) and the closely related woodchuck hepatitis virus (WHV) are potent carcinogens that trigger development of primary hepatocellular carcinoma (HCC). The initial sites of hepadnavirus-host genome integration, their diversity and kinetics of formation can be central to virus persistence and the initiation and progression of HCC. To recognize the nature of the very early virus-host interactions, we explored de novo infection of human hepatocyte-like HepaRG cells with authentic HBV and naive woodchucks with WHV...
April 17, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28408542/acyl-coa-lysophosphatidylethanolamine-acyltransferase-activity-affects-growth
#4
Katarzyna Jasieniecka-Gazarkiewicz, Ida Lager, Anders Carlsson, Katharina Gutbrod, Helga Peisker, Peter Dörmann, Sten Stymne, Antoni Banas
Arabidopsis contains two enzymes (encoded by the At1g80950 and At2g456702 genes) preferentially acylating lyso-phosphatidylethanolamine (LPE) with acyl-CoA, designated lyso-phosphatidylethanolamine acyltransferase 1 (LPEAT1) and lyso-phosphatidylethanolamine acyltransferase 2 (LPEAT2). T-DNA insertion mutants of lpeat2 and double mutants of lpeat1 lpeat2 showed impaired growth, smaller leaves, shorter roots, less seed setting and reduced lipid content per FW in roots and seeds and large increases in LPE and lyso-phosphatidylcholine (LPC) contents in leaves...
April 13, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28402957/chromatin-state-dynamics-during-nk-cell-activation
#5
Yang Li, Jin Wang, Jie Yin, Xinhua Liu, Minghang Yu, Ting Li, Han Yan, Xi Wang
Studies of Natural Killer (NK) cell cytotoxicity have mainly focused on the balance of activating and inhibitory receptors, signaling transduction, calcium influx, formation of immune synapse, and cytolytic degranulation. However, little is known about the chromatin state of NK cells and the impact of its changes during target recognition. In this study, we investigate the contribution of chromatin state dynamics during NK cell activation by comprehensively analyzing a set of microarray data and two sets of Chromatin Immunoprecipitation-Sequencing (ChIP-seq) data...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28397772/gold-nanoparticles-deposited-on-surface-modified-carbon-xerogels-as-reusable-catalysts-for-cyclohexane-c-h-activation-in-the-presence-of-co-and-water
#6
Ana Paula da Costa Ribeiro, Luísa Margarida Dias Ribeiro de Sousa Martins, Sónia Alexandra Correia Carabineiro, José Luís Figueiredo, Armando José Latourrette Pombeiro
The use of gold as a promotor of alkane hydrocarboxylation is reported for the first time. Cyclohexane hydrocarboxylation to cyclohexanecarboxylic acid (up to 55% yield) with CO, water, and peroxodisulfate in a water/acetonitrile medium at circa 50 °C has been achieved in the presence of gold nanoparticles deposited by a colloidal method on a carbon xerogel in its original form (CX), after oxidation with HNO₃ (-ox), or after oxidation with HNO₃ and subsequent treatment with NaOH (-ox-Na). Au/CX-ox-Na behaves as re-usable catalyst maintaining its initial activity and selectivity for at least seven consecutive cycles...
April 9, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28393568/assessment-of-radical-scavenging-whitening-and-moisture-retention-activities-of-panax-ginseng-berry-mediated-gold-nanoparticles-as-safe-and-efficient-novel-cosmetic-material
#7
Zuly Jiménez, Yeon-Ju Kim, Ramya Mathiyalagan, Kwang-Hoon Seo, Padmanaban Mohanan, Jong-Chan Ahn, Yu-Jin Kim, Deok Chun Yang
Panax ginseng berry extract possess remarkable pharmacological effects on skin treatment such as anti-aging, antioxidant, promotor of collagen synthesis and alleviation against atopic dermatitis. In recent years, gold nanoparticles have gained much attention due to their extensive range of applications in particular in the field of drug delivery as a result of their biological compatibility and low toxicity. In a previous study, we designed and developed biocompatible gold and silver nanoparticles based on phytochemical profile and pharmacological efficacy of P...
April 9, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28391873/il-10-and-il-28b-gene-variants-as-predictors-of-sustained-response-to-peginterferon-and-ribavirin-therapy-in-chronic-hcv-infection
#8
Ikram Sghaier, Leila Mouelhi, Noor A Rabia, Ezzedine Ghazoueni, Wassim Y Almawi, Besma Yacoubi Loueslati
OBJECTIVES: Interleukin-10 (IL-10) plays an important role in the immunity to hepatitis C virus (HCV). Insofar as IL-10 variants are associated with altered levels of IL-10, previous studies that examined the association of IL-10 polymorphisms with the susceptibility to and progression of chronic HCV, and response to anti-viral treatment were inconsistent. We investigated the association between common IL-10 variants in the intron and the promotor region with HCV and associated features...
April 5, 2017: Cytokine
https://www.readbyqxmd.com/read/28388578/the-tumor-suppressor-interferon-regulatory-factor-8-inhibits-%C3%AE-catenin-signaling-in-breast-cancers-but-is-frequently-silenced-by-promoter-methylation
#9
Xinrong Luo, Xin Xiong, Qing Shao, Tingxiu Xiang, Lili Li, Xuedong Yin, Xia Li, Qian Tao, Guosheng Ren
Interferon (IFN) regulatory factor 8 is encoded by a novel candidate tumor suppressor gene (IRF8), its promotor is frequently methylated in multiple cancers. However, the promoter methylation status, functions and underlying mechanisms of IRF8 in breast cancer remain unclear. We found that IRF8 was downregulated in breast cancer cell lines and primary tumors, compared with normal breast tissues, mainly because of aberrant promoter methylation. However, its expression was not associated with pathological characteristics...
March 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28388042/triflic-acid-promoted-decarboxylation-of-adamantane-oxazolidine-2-one-access-to-chiral-amines-and-heterocycles
#10
Radim Hrdina, Marta Larrosa, Christian Logemann
We have developed a one-step procedure to a variety of chiral lipophilic and conformationally rigid amines and heterocycles by decarboxylation of adamantane-oxazolidine-2-one. Triflic acid or aluminum triflate promote the addition of diverse nucleophiles to oxazolidine-2-one moiety accompanied by the release of carbon dioxide. The resulting amine or heterocycle is then protonated/metalated by the catalyst (promotor). Additionally, the starting racemic material, adamantane-oxazolidine-2-one, was resolved into single enantiomers using a chiral auxiliary to access enantio-enriched products and to study the racemization pathway of chiral 1,2-disubstituted adamantane derivatives...
April 7, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28375699/vida-pura-an-assessment-of-the-fidelity-of-promotor-delivered-screening-and-brief-intervention-to-reduce-unhealthy-alcohol-use-among-latino-day-laborers
#11
Samantha E Serrano, Kelly Serafini, Nikki Eller, Vanessa N Torres, Dennis Donovan, India J Ornelas
We assessed the fidelity of promotores conducting screening and brief intervention (SBI) to reduce unhealthy alcohol use among Latino immigrant day laborers in the Vida PURA study. We reviewed 32 audio-recorded brief interventions to assess promotor adherence to the intervention protocol and to evaluate their motivational interviewing (MI) technique with the Motivational Interviewing Treatment Integrity (MITI) 4.2.1 tool. Promotores delivered three core intervention steps in 78% of recordings and achieved basic MI competence across all domains and proficiency in 50% of measures...
April 4, 2017: Journal of Ethnicity in Substance Abuse
https://www.readbyqxmd.com/read/28367517/evaluation-of-tumorigenic-potential-of-ceo2-and-fe2o3-engineered-nanoparticles-by-a-human-cell-in-vitro-screening-model
#12
Todd A Stueckle, Donna C Davidson, Raymond Derk, Tiffany G Kornberg, Diane Schwegler-Berry, Sandra V Pirela, Glen Deloid, Philip Demokritou, Sudjit Luanpitpong, Yon Rojanasakul, Liying Wang
With rapid development of novel nanotechnologies that incorporate engineered nanomaterials (ENMs) into manufactured products, long-term, low dose ENM exposures in occupational settings is forecasted to occur with potential adverse outcomes to human health. Few ENM human health risk assessment efforts have evaluated tumorigenic potential of ENMs. Two widely used nano-scaled metal oxides (NMOs), cerium oxide (nCeO2) and ferric oxide (nFe2O3) were screened in the current study using a sub-chronic exposure to human primary small airway epithelial cells (pSAECs)...
April 2017: NanoImpact
https://www.readbyqxmd.com/read/28360421/muscle-specific-downregulation-of-gr-levels-inhibits-adipogenesis-in-porcine-intramuscular-adipocyte-tissue
#13
Weiwei Chu, Wei Wei, Haiyin Han, Ying Gao, Kaiqing Liu, Ye Tian, Zaohang Jiang, Lifan Zhang, Jie Chen
Intramuscular adipose is conducive to good pork quality, whereas subcutaneous adipose is considered as waste in pig production. So uncovering the regulation differences between these two adiposes is helpful to tissue-specific control of fat deposition. In this study, we found the sensitivity to glucocorticoids (GCs) was lower in intramuscular adipocytes (IMA) compared with subcutaneous adipocytes (SA). Comparison of glucocorticoid receptor (GR) revealed that IMA had lower GR level which contributed to its reduced GCs sensitivity...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28353642/dysregulated-ier3-expression-is-associated-with-enhanced-apoptosis-in-titin-based-dilated-cardiomyopathy
#14
Qifeng Zhou, Julia Kelley Hahn, Balram Neupane, Parwez Aidery, Siegfried Labeit, Meinrad Gawaz, Michael Gramlich
Apoptosis (type I programmed cell death) of cardiomyocytes is a major process that plays a role in the progression of heart failure. The early response gene IER3 regulates apoptosis in a wide variety of cells and organs. However, its role in heart failure is largely unknown. Here, we investigate the role of IER3 in an inducible heart failure mouse model. Heart failure was induced in a mouse model that imitates a human titin truncation mutation we found in a patient with dilated cardiomyopathy (DCM). Transferase dUTP nick end labeling (TUNEL) and ssDNA stainings showed induction of apoptosis in titin-deficient cardiomyocytes during heart failure development, while IER3 response was dysregulated...
March 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28352655/panobinostat-acts-synergistically-with-ibrutinib-in-diffuse-large-b-cell-lymphoma-cells-with-myd88-l265-mutations
#15
Patrizia Mondello, Elliott J Brea, Elisa De Stanchina, Eneda Toska, Aaron Y Chang, Myles Fennell, Venkatraman Seshan, Ralph Garippa, David A Scheinberg, José Baselga, Hans-Guido Wendel, Anas Younes
Diffuse large B cell lymphoma (DLBCL) frequently harbors genetic alterations that activate the B cell receptor (BCR) and TLR pathways, which converge to activate NF-κB. While selective inhibition of BTK with ibrutinib causes clinical responses in relapsed DLBCL patients, most responses are partial and of a short duration. Here, we demonstrated that MyD88 silencing enhanced ibrutinib efficacy in DLBCL cells harboring MyD88 L265P mutations. Chemical downregulation of MyD88 expression with HDAC inhibitors also synergized with ibrutinib...
March 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28336461/chloroform-free-permeabilization-for-improved-detection-of-%C3%AE-galactosidase-activity-in-vibrio-cholerae
#16
Charlotte Toulouse, Claudia C Häse, Julia Steuber
LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase of LacZ activities compared to lysis with chloroform.
March 21, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28334739/hiv-treatment-as-prevention-implications-for-health-promotion
#17
Sean Slavin
Evidence has been accumulating in recent years that individuals with HIV who are effectively treated with antiretroviral drugs are highly unlikely to transmit the virus to their sexual partners. This approach has great potential to contribute to ongoing prevention efforts and has been termed 'treatment as prevention'. International political momentum has been building in recent years to attempt to significantly reduce new HIV infections globally and end the epidemic by 2030. In pursuit of such aims a number of local health authorities and communities have adopted intensified 'test and treat' strategies to quickly diagnose infections and reduce the risk of onward transmission through an immediate offer of treatment...
October 8, 2016: Health Promotion International
https://www.readbyqxmd.com/read/28330874/high-resolution-3d-nmr-structure-of-the-kras-proto-oncogene-promoter-reveals-key-features-of-a-g-quadruplex-involved-in-transcriptional-regulation
#18
Abdelaziz Kerkour, Julien Marquevielle, Stefaniia Ivashchenko, Liliya A Yatsunyk, Jean-Louis Mergny, Gilmar F Salgado
Non-canonical base pairing within guanine-rich DNA and RNA sequences can produce G-quartets, whose stacking leads to the formation of a G-quadruplex (G4). G4s can coexist with canonical duplex DNA in the human genome and have been suggested to suppress gene transcription, and much attention has therefore focused on studying G4s in promotor regions of disease-related genes. For example, the human KRAS proto-oncogene contains a nuclease-hypersensitive element (1) located upstream of the major transcription start site...
March 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28323677/transferable-training-modules-building-environmental-education-opportunities-with-and-for-mexican-community-health-workers-promotores-de-salud
#19
Denise Moreno Ramírez, Lourdes Vea, James A Field, Paul B Baker, A Jay Gandolfi, Raina M Maier
Community health workers (promotores de salud) have the ability to empower communities to mitigate negative health outcomes. Current training efforts in environmental topics are lacking. This project addressed this gap by developing 4 transferable training modules on environmental health. By applying a series of surveys, interviews, and trainings, we evaluated their relevance. Partners provided favorable feedback for 3 of the 4 modules. It was also learned that the development method could be improved by engaging technically trained promotores de salud in the role of co-creators...
March 20, 2017: Family & Community Health
https://www.readbyqxmd.com/read/28319682/n-substituted-5-amino-1-hydroxymethyl-cyclopentanetriols-a-new-family-of-activity-promotors-for-a-gm1-gangliosidosis-related-human-lysosomal-%C3%AE-galactosidase-mutant
#20
Michael Schalli, Christina Tysoe, Roland Fischer, Bettina M Pabst, Martin Thonhofer, Eduard Paschke, Tanja Rappitsch, Arnold E Stütz, Marion Tschernutter, Werner Windischhofer, Stephen G Withers
From 1,2;3,4-di-O-isopropylidene-α-D-galactopyranose, a series of highly functionalized (hydroxymethyl)cyclopentanes was easily available. In line with reports by Reymond and Jäger on similar structures, these amine containing basic carbasugars are potent inhibitors of β-D-galactosidases and, for the first time, could be shown to act as pharmacological chaperones for GM1-gangliosidosis-associated lysosomal acid β-galactosidase mutant R201C, thus representing a new structural type of pharmacological chaperones for this lysosomal storage disease...
March 11, 2017: Carbohydrate Research
keyword
keyword
38889
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"