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Fırat Kurt, Ertugrul Filiz
Iron (Fe) is an essential element for plant life. Its deficiency impedes growth and development and excessive iron can cause the toxic effect via the Fenton reaction. Thus, plants have developed various mechanisms to acquire, distribute and utilize Fe for the maintenance of their iron homeostasis at cellular and systemic levels. A basic helix-loop-helix (bHLH) transcription factor family plays essential roles in many regulatory and development processes in plants. In this study, we aimed to understand the roles of bHLH38, bHLH39, bHLH100 and bHLH101 genes for Fe homeostasis in Arabidopsis, tomato, rice, soybean and maize species by using bioinformatics approaches...
March 15, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Telma Moreira, Daphne C Hernandez, Claudia W Scott, Rosenda Murillo, Elizabeth M Vaughan, Craig A Johnston
Hispanics are disparately affected by diabetes. Treating socioeconomically disadvantaged Hispanics is challenging due to economic and cultural barriers. Health care providers must understand that cultural beliefs about medicine and disease may have an impact on how diabetes treatment is viewed. Concepts such as susto (fright), coraje (anger), and fatalismo (fatalism) are common cultural beliefs. If these beliefs are not well understood by the health care provider, recommendations for treatment are likely to be discarded...
January 2018: American Journal of Lifestyle Medicine
Katrin Schelleckes, Boris Schmitz, Malte Lenders, Mirja Mewes, Stefan-Martin Brand, Eva Brand
KIBRA has been suggested as a key regulator of the hippo pathway, regulating organ size, cell contact inhibition as well as tissue regeneration and tumorigenesis. Recently, alterations of KIBRA expression caused by promotor methylation have been reported for several types of cancer. Our current study aimed to design an artificial transcription factor capable of re-activating expression of the tumor suppressor KIBRA and the hippo pathway. We engineered a new gene named 'ZFP226' encoding for a ~23 kDa fusion protein...
March 9, 2018: Scientific Reports
Maozhi Hu, Weixin Zhao, Hongying Li, Jie Gu, Qiuxiang Yan, Xiaohui Zhou, Zhiming Pan, Guiyou Cui, Xinan Jiao
BACKGROUND: Enhancing caspase-1 activation in macrophages is helpful for the clearance of intracellular bacteria in mice. Our previous studies have shown that EscI, an inner rod protein of type III system in E. coli can enhance caspase-1 activation. The purpose of this study was to further analyze the prospect of EscI in the vaccine design. RESULTS: A recombinant Salmonella expressing SspH2-EscI fusion protein using the promotor of Salmonella effector SspH2, X4550(pYA3334-P-SspH2-EscI), was constructed...
March 9, 2018: BMC Veterinary Research
Margo J H van Campenhout, Florian van Bömmel, Maria Pfefferkorn, Janett Fischer, Danilo Deichsel, André Boonstra, Anneke J van Vuuren, Thomas Berg, Bettina E Hansen, Harry L A Janssen
Hepatitis B virus (HBV) RNA in serum is a novel biomarker for intrahepatic HBV replication and treatment response. For its proper use it is essential to identify factors influencing serum HBV RNA level. Using a RACE PCR technique (lower limit of detection 800 copies/mL [c/mL]), serum HBV RNA levels were measured in samples of 488 untreated individuals with chronic HBV infection who were eligible to treatment according to currently used recommendations. We explored the association of serum levels of HBV RNA with patient and virus associated factors...
March 7, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Annelies Verbiest, Diether Lambrechts, Thomas Van Brussel, Gabrielle Couchy, Agnieszka Wozniak, Arnaud Méjean, Evelyne Lerut, Stéphane Oudard, Virginie Verkarre, Sylvie Job, Aurélien de Reynies, Jean-Pascal Machiels, Jean-Jacques Patard, Jessica Zucman-Rossi, Benoit Beuselinck
BACKGROUND: Clear-cell renal-cell carcinoma (ccRCC) is characterized by loss of a functional Von Hippel-Lindau (VHL) protein. We investigated the potential of 3 single nucleotide polymorphisms (SNPs) in VHL as biomarkers in metastatic ccRCC (m-ccRCC) patients treated with vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitors (TKIs). PATIENTS AND METHODS: We genotyped 3 VHL SNPs in 199 m-ccRCC patients: rs1642742 T > C, rs1642743 A > G, and rs1678607 C > A...
February 5, 2018: Clinical Genitourinary Cancer
Zhengxi Wei, Zhongguo Shan, Zahir A Shaikh
Epidemiological and experimental studies have implicated cadmium (Cd) with breast cancer. In breast cancer-derived cells, Cd has been shown to promote MCF10A and MDA-MB-231 cell growth. The present study examined whether Cd also causes epithelial-mesenchymal transition (EMT), a hallmark of cancer progression. Human breast epithelial cells consisting of non-cancerous MCF10A, non-metastatic HCC 1937 and HCC 38, and metastatic MDA-MB-231 were treated with 1 or 3 μM Cd for 4 weeks. The MCF10A epithelial cells switched to a more mesenchymal-like morphology, which was accompanied by a decrease in the epithelial marker E-cadherin and an increase in the mesenchymal markers N-cadherin and vimentin...
February 28, 2018: Toxicology and Applied Pharmacology
Alba Pablos-Tanarro, Daniel Lozano-Ojalvo, Elena Molina, Rosina López-Fandiño
This work aimed to assess the contribution of the major egg white proteins, ovalbumin, ovomucoid and lysozyme, to the induction and elicitation of allergenic responses. For this purpose, BALB/c mice were orally administered either the individual egg allergens or a mixture of the three proteins in the same proportion, in order to evaluate their relative allergenicity avoiding their different abundance in egg white. Cholera toxin was used as a Th2-polarizing adjuvant. Ovomucoid and lysozyme triggered the most severe anaphylaxis reactions upon oral challenge...
March 1, 2018: Journal of Agricultural and Food Chemistry
Dong Luo, Mian Li, Xiao-Ping Zhou, Dan Li
A set of planar organic fluorophores (R = benzene, naphthalene, pyrene) and luminescence promotors (X = Cl, Br, I) are equipped, respectively, on the alternatively-arranged vertices of cubic Zn(II)-imidazolate cages through orthogonal subcomponent self-assembly. It is found that supramolecular interactions, especially weak CHX interactions, play a key role in activating an efficient radiative pathway, coupled with reduced nonradiative decay rate through metal coordination, therefore significantly boosting the emission quantum yields of the system...
February 28, 2018: Chemistry: a European Journal
Yunfeng Fu, Chengyuan Li, Yanwei Luo, Lian Li, Jing Liu, Rong Gui
Long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) was recently identified as oncogene in several cancers. However, the role of MIAT on acquired resistance in lung cancer and the underlying mechanisms remain unclear. Here, we showed that the expression of MIAT in lung cancer tissues was upregulated compared with adjacent tissues. LncRNA MIAT expression was associated with tumor size, lymph node metastasis, distant metastasis and TNM stage. Univariate analysis and multivariate analysis revealed that the lncRNA MIAT to be an independent factor for predicating the prognosis of lung cancer patients...
2018: Frontiers in Pharmacology
Miao Wang, Lyu Zhang, Zeyang Liu, Jiamin Zhou, Qi Pan, Jia Fan, Rongyu Zang, Lu Wang
AGO1 is a major component of RNA-induced silencing complexes and plays a crucial role in solid tumors. The aim of our study was to investigate AGO1 functions in hepatocellular carcinoma (HCC). Using small interfering RNA, AGO1 functions were investigated in HCCLM3 cell lines. Cell proliferation, immigration, and invasion significantly decreased after AGO1 depletion using MTT, wound-healing, and transwell assay. The associated proteins in the epithelial-mesenchymal transition (EMT) and the activation of its signal pathways were measured using western blot...
February 27, 2018: Cell Death & Disease
Won Sub Kang, Young Jong Kim, Hae Jeong Park, Su Kang Kim, Jong-Woo Paik, Jong Woo Kim
BACKGROUND: Immunological alterations and dysregulation of the inflammatory response have been suggested to play a crucial role in schizophrenia pathophysiology. Growing evidence supports the involvement of chemokines in brain development, thus many chemokines have been studied in relation with schizophrenia. The C-C motif chemokine ligand 11 (CCL11) has been shown to be related with synaptic plasticity and neurogenesis. Moreover, altered levels of CCL11 have been observed in schizophrenia patients...
February 22, 2018: Gene
Zarqa Nawaz, Kaleem U Kakar, Raqeeb Ullah, Shizou Yu, Jie Zhang, Qing-Yao Shu, Xue-Liang Ren
Tobacco (Nicotiana tabacum) serve as the top leading commercial, non-food, and model crop worldwide. Cyclic nucleotide-gated channels (CNGCs) are ligand-gated, calcium-permeable, divalent, cation-selective channels, involved in important biological functions. Here, we systematically characterized thirty-five CNGC genes in the genome of Nicotiana tabacum, and classified into four phylogenetic groups. Evolutionary analysis showed that NtabCNGC family of N. tabacum originated from the parental genome of N. sylvestris and N...
February 21, 2018: Genomics
Dhivya Thiyagarajan, Hege L Pedersen, Natalya Seredkina, Kjersti D Horvei, Lorena Arranz, Ramon Sonneveld, Tom Nijenhuis, Johan van der Vlag, Ole P Rekvig
Recently we described that endonuclease inactive DNase I translocated into the nucleus in response to increased endogenous IL-1β expression. Here, we demonstrate impact and function of translocated DNase I in tubular cells. Effect of cytokines on expression level and nuclear localisation of DNase I and corresponding levels of Fas receptor (FasR) and IL-1β were determined by confocal microscopy, qPCR and western blot analyses, in presence or absence of siRNA against IL-1β and DNase I mRNA. Nuclear DNase I bound to the FAS promotor region as determined by chromatin immuno-precipitation analysis...
2018: Frontiers in Cell and Developmental Biology
Stephanie E Combs, Kerstin A Kessel, Josefine Hesse, Christoph Straube, Claus Zimmer, Friederike Schmidt-Graf, Jürgen Schlegel, Jens Gempt, Bernhard Meyer
BACKGROUND: Generally, re-irradiation (Re-RT) is offered to patients with glioma recurrences with macroscopic lesions. Results are discussed controversially, and some centers postulate limited benefit of Re-RT. Re-RT is generally offered to tumors up to 4 cm in diameter. Re-resection is also discussed controversially; however, recent studies have shown significant benefit. OBJECTIVE: To combine proactive re-resection and early Re-RT in patients with recurrent glioma...
February 16, 2018: Neurosurgery
Evangelia Petridou, Christel Barker Jensen, Athanasios Arvanitidis, Maria Giannaki-Psinaki, Athanasios Michos, Karen Angeliki Krogfelt, Randi Føns Petersen
PURPOSE: To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY: Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 real-time PCR confirmed Bordetella spp./B. pertussis infection in 300 samples. A subset of samples (n=153) were analysed by multi-locus variable number tandem repeat analysis (MLVA) and (n=25) by sequence-based typing of the toxin promotor region (ptxP) on DNA extracted from clinical specimens...
February 2, 2018: Journal of Medical Microbiology
Dennis Nurjadi, Klaus Heeg, Alexander N R Weber, Philipp Zanger
OBJECTIVES: Toll-like receptor (TLR) -9 could have importance in human S. aureus immunity, but population level evidence for this hypothesis is missing. METHODS: We phenotyped 603 volunteers for S. aureus nasal carriage using four consecutive swabs, genotyped TLR9 promotor variants in 106 persistent carriers and 219 non-carriers, measured TLR9-mRNA expression in whole blood after stimulation with viable S. aureus, and studied mutual associations of carriage, transcriptional activity, and single nucleotide polymorphisms while accounting for sex and hormonal contraceptive use (HCU+/- )...
February 16, 2018: Clinical Microbiology and Infection
Mengmeng Lu, Chuanyan Yang, Meijia Li, Qilin Yi, Guangxia Lu, Yichen Wu, Chen Qu, Lingling Wang, Linsheng Song
Interferon regulatory factors (IRFs), a family of transcription factors with a novel helix-turn-helix DNA-binding motif, play important roles in regulating the expression of interferons (IFNs) and IFN-stimulated genes. In the present study, an interferon regulation factor 1 was identified from oyster Crassostrea gigas (designated CgIRF-1), and its immune function was characterized to understand the regulatory mechanism of interferon system against viral infection in invertebrates. The open reading frame (ORF) of CgIRF-1 was 993 bp, encoding a polypeptide of 330 amino acids with a typical IRF domain (also known as DNA-binding domain)...
February 16, 2018: Fish & Shellfish Immunology
N Pospiech, H Cibis, L Dietrich, F Müller, T Bange, S Hennig
Interactions of long non-coding RNAs (lncRNA) with proteins play important roles in the regulation of many cellular processes. PANDAR (Promotor of CDKN1A Antisense DNA damage Activated RNA) is a lncRNA that is transcribed in a p53-dependent manner from the CDKN1A promoter and is involved in the regulation of proliferation and senescence. Overexpression of PANDAR has been observed in several tumor species and correlated with a poor prognosis for patient survival rate. Depending on the cellular state, PANDAR is known to interact with proteins such as the nuclear transcription factor Y subunit A (NF-YA) and the scaffold attachment factor A (SAF-A)...
February 12, 2018: Scientific Reports
Roser Ufartes, Janina Schwenty-Lara, Luisa Freese, Christiane Neuhofer, Janika Möller, Peter Wehner, Conny M A van Ravenswaaij-Arts, Monica T Y Wong, Ina Schanze, Andreas Tzschach, Oliver Bartsch, Annette Borchers, Silke Pauli
CHARGE syndrome is an autosomal dominant malformation disorder caused by heterozygous loss of function mutations in the chromatin remodeler CHD7. Chd7 regulates the expression of Sema3a, which also contributes to the pathogenesis of Kallmann syndrome, a heterogeneous condition with the typical features hypogonadotropic hypogonadism and an impaired sense of smell. Both features are common in CHARGE syndrome suggesting that SEMA3A may provide a genetic link between these syndromes. Indeed, we find evidence that SEMA3A plays a role in the pathogenesis of CHARGE syndrome...
February 8, 2018: Human Molecular Genetics
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