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https://www.readbyqxmd.com/read/28840818/polycladomyces-subterraneus-sp-nov-isolated-from-soil-in-thailand
#1
Narisara Maneewong, Chatsuda Sakdapetsiri, Chanwit Suriyachadkun, Chiyo Shibata, Tomohiko Tamura, Shinji Tokuyama, Vichien Kitpreechavanich
A thermophilic poly(L-lactide)-degrading Gram-stain-positive filamentous bacterial strain that develops single spores on the aerial mycelium was isolated from forest soil at Srinagarind Dam, Kanchanaburi Province, Thailand. The results of a polyphasic taxonomic study showed that our isolate had characteristics typical of members of the genus Polycladomyces. The isolate grew aerobically at an optimum temperature of 50-55 °C and optimal pH 6-7. Meso-diaminopimelic acid was present as the diagnostic diamino acid in the peptidoglycan but no characteristic sugars are detected...
August 25, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28673682/efficient-neural-differentiation-of-mouse-pluripotent-stem-cells-in-a-serum-free-medium-and-development-of-a-novel-strategy-for-enrichment-of-neural-cells
#2
Isha Verma, Zubin Rashid, Sujit K Sikdar, Polani B Seshagiri
Pluripotent stem cells (PSCs) offer an excellent model to study neural development and function. Although various protocols have been developed to direct the differentiation of PSCs into desired neural cell types, many of them suffer from limitations including low efficiency, long duration of culture, and the use of expensive, labile, and undefined growth supplements. In this study, we achieved efficient differentiation of mouse PSCs to neural lineage, in the absence of exogenous molecules, by employing a serum-free culture medium containing knockout serum replacement (KSR)...
July 1, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28656543/ramucirumab-for-treating-advanced-gastric-cancer-or-gastro-oesophageal-junction-adenocarcinoma-previously-treated-with-chemotherapy-an-evidence-review-group-perspective-of-a-nice-single-technology-appraisal
#3
REVIEW
Nasuh C Büyükkaramikli, Hedwig M Blommestein, Rob Riemsma, Nigel Armstrong, Fiona J Clay, Janine Ross, Gill Worthy, Johan Severens, Jos Kleijnen, Maiwenn J Al
The National Institute for Health and Care Excellence (NICE) invited the company that manufactures ramucirumab (Cyramza(®), Eli Lilly and Company) to submit evidence of the clinical and cost effectiveness of the drug administered alone (monotherapy) or with paclitaxel (combination therapy) for treating adults with advanced gastric cancer or gastro-oesophageal junction (GC/GOJ) adenocarcinoma that were previously treated with chemotherapy, as part of the Institute's single technology appraisal (STA) process...
June 27, 2017: PharmacoEconomics
https://www.readbyqxmd.com/read/28566647/potential-use-of-polyvinyl-acetate-polyvinylpyrrolidone-mixture-for-the-development-of-atenolol-sustained-release-matrix-tablets-optimization-of-formulation-through-in-vitro-in-vivo-assessment-study
#4
Ali Saeed Owayez, Galal Mahmoud Abd El-Ghany, Irhan Ibrahim Abu Hashim
The objective of this study was to develop sustained release matrix tablets of atenolol (AT) using different concentrations of polyvinyl acetate-polyvinylpyrrolidone mixture (KSR) (20, 30, or 40%) with various types of fillers such as spray dried lactose (SP.D.L), avicel pH 101 (AV), and emcompress (EMS). The physical characteristics of the prepared tablets were evaluated. Characterization of the optimized formulation was performed using Fourier transform (FT)-IR spectroscopy and differential scanning calorimetry (DSC)...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28511995/live-full-term-mouse-pups-from-oocytes-grown-and-matured-in-vitro-with-serum-substitutes
#5
Hideyuki H Motohashi, Ryoma Taniguchi, Junpei Sakamoto, Tadashi Sankai, Hidemi Kada
For in vitro growth and maturation of mouse oocytes (IVG-IVM), serum is added to media up to and including the stage of oocyte maturation; this subsequently supports oocytes through fertilization and early embryo development. However, problems may occur with sera, such as batch differences and issues of biosafety. The purpose of the present study was to determine the capacity for fertilization and pre- and post-implantation development of oocytes that underwent IVG-IVM with a serum substitute. Oocyte-granulosa cell complexes from preantral follicles were cultured in medium with either fetal bovine serum (FBS), Serum Substitute Supplement™ (SSS), or Knockout™ Serum Replacement (KSR) for 10days, and were then allowed to mature for 17 h...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28500680/rapid-detoxification-via-glutathione-s-transferase-gst-conjugation-confers-high-level-of-atrazine-resistance-in-palmer-amaranth-amaranthus-palmeri
#6
Sridevi Nakka, Amar S Godar, Curtis R Thompson, Dallas E Peterson, Mithila Jugulam
BACKGROUND: Palmer amaranth (Amaranthus palmeri) is an economically troublesome, aggressive and damaging weed that has evolved resistance to six herbicide modes of action (MOA) including photosystem II (PS II)-inhibitors, such as atrazine. The objective of this study was to investigate the mechanism and inheritance of atrazine resistance in Palmer amaranth. RESULTS: A population of Palmer amaranth from Kansas (KSR) had a high level (160-198 fold more; SE ± 21-26) of resistance to atrazine compared to the two known susceptible populations MSS and KSS, from Mississippi and KS, respectively...
May 13, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28488216/knock-out-serum-replacement-and-melatonin-effects-on-germ-cell-differentiation-in-murine-testicular-explant-cultures
#7
Ahmed Reda, Halima Albalushi, Sheyla Cisneros Montalvo, Mirja Nurmio, Zeliha Sahin, Mi Hou, Niels Geijsen, Jorma Toppari, Olle Söder, Jan-Bernd Stukenborg
Finding robust culture conditions for in vitro maturation (IVM) of male germ cells is still a challenge. Recently, a testis organ culture method, using Knockout Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes were cut into small pieces which were cultured atop agarose stands, using Minimum Essential Medium alpha supplemented with different supplements; melatonin, Glutamax, and different concentrations of KSR...
July 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28477135/lentiviral-reprogramming-of-a-t-patient-fibroblasts-to-induced-pluripotent-stem-cells
#8
Sam Nayler, Sergei V Kozlov, Martin F Lavin, Ernst Wolvetang
Reprogramming of cells enables generation of pluripotent stem cells and resulting progeny through directed differentiation, making this technology an invaluable tool for the study of human development and disease. Reprogramming occurs with a wide range of efficiency, a culmination of intrinsic and extrinsic factors including the tissue of origin, the passage number and culture history of the target cells. Another major factor affecting reprogramming is the methodology used and the quality of the reprogramming process itself, including for conventional viral-based approaches viral titer and subsequent viral transduction efficiency, including downstream transgene insertion and stoichiometry...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28474535/long-term-propagation-of-porcine-undifferentiated-spermatogonia
#9
Pengfei Zhang, Xiaoxu Chen, Yi Zheng, Jinshen Zhu, Yuwei Qin, Yinghua Lv, Wenxian Zeng
Spermatogonial stem cells (SSCs) provide the foundation for spermatogenesis and fertility throughout the adult life of a male. Genetic manipulations of SSCs combined with germ cell transplantation present a novel approach for gene therapy and production of genetically modified animals. However, the rarity of SSCs within mammalian testes remains an impediment to related applications, making in vitro expansion of SSCs a prerequisite. Nevertheless, long-term culture systems of SSCs from large animals have not been established yet...
August 1, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28443128/physiological-and-molecular-characterization-of-hydroxyphenylpyruvate-dioxygenase-hppd-inhibitor-resistance-in-palmer-amaranth-amaranthus-palmeri-s-wats
#10
Sridevi Nakka, Amar S Godar, Prashant S Wani, Curtis R Thompson, Dallas E Peterson, Jeroen Roelofs, Mithila Jugulam
Herbicides that inhibit hydroxyphenylpyruvate dioxygenase (HPPD) such as mesotrione are widely used to control a broad spectrum of weeds in agriculture. Amaranthus palmeri is an economically troublesome weed throughout the United States. The first case of evolution of resistance to HPPD-inhibiting herbicides in A. palmeri was documented in Kansas (KS) and later in Nebraska (NE). The objective of this study was to investigate the mechansim of HPPD-inhibitor (mesotrione) resistance in A. palmeri. Dose response analysis revealed that this population (KSR) was 10-18 times more resistant than their sensitive counterparts (MSS or KSS)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28401100/generation-of-hematopoietic-stem-cells-from-human-embryonic-stem-cells-using-a-defined-stepwise-serum-free-and-serum-replacement-free-monolayer-culture-method
#11
So-Jung Kim, Ji-Won Jung, Hye-Yeong Ha, Soo Kyung Koo, Eung-Gook Kim, Jung-Hyun Kim
BACKGROUND: Embryonic stem cells (ESCs) can be expanded infinitely in vitro and have the potential to differentiate into hematopoietic stem cells (HSCs); thus, they are considered a useful source of cells for HSC production. Although several technical in vitro methods for engineering HSCs from pluripotent stem cells have been developed, clinical application of HSCs engineered from pluripotent stem cells is restricted because of the possibility of xenogeneic contamination resulting from the use of murine materials...
March 2017: Blood Research
https://www.readbyqxmd.com/read/28398669/evaluation-of-culture-time-and-media-in-an-in-vitro-testis-organ-culture-system
#12
Noriko Nakamura, Gwenn E Merry, Amy L Inselman, Daniel T Sloper, Pedro L Del Valle, Takuya Sato, Takehiko Ogawa, Deborah K Hansen
BACKGROUND: The complexity of spermatogenesis makes development of appropriate in vitro testis models challenging. A novel in vitro mouse testis culture system has been reported but not yet evaluated as an alternative model for male reproductive toxicity testing. We assessed the effects of media composition on sperm differentiation and testis morphology of cultured mouse testis fragments. METHODS: Testes from postnatal day 5 B6:CBA-Tg(Acrv1-EGFP)2727Redd/J male mice were cultured in knockout serum replacement (KSR) or Albumax I (Albumax) medium...
April 17, 2017: Birth defects research
https://www.readbyqxmd.com/read/28397607/sex-reversal-and-analyses-of-possible-involvement-of-sex-steroids-in-scallop-gonadal-development-in-newly-established-organ-culture-systems
#13
Ayano Otani, Tadaaki Nakajima, Tomomi Okumura, Shiro Fujii, Yasuhiro Tomooka
Many molluscs perform sex reversal, and sex hormones may be involved in the process. In adult scallops, Patinopecten yessoensis, gonadotropin releasing hormone and 17β-estradiol (E2) are involved in male sexual maturation, however, little is known about the effects of E2 and testosterone (T) on the gonadal differentiation in young scallops. In the present study, scallop gonadal development was analyzed to determine the sex reversal stage in Funka bay, and effects of E2 and T were examined. In Funka bay, almost all scallops were male at month 12...
April 2017: Zoological Science
https://www.readbyqxmd.com/read/28365113/associations-between-pathogen-specific-cases-of-subclinical-mastitis-and-milk-yield-quality-protein-composition-and-cheese-making-traits-in-dairy-cows
#14
T Bobbo, P L Ruegg, G Stocco, E Fiore, M Gianesella, M Morgante, D Pasotto, G Bittante, A Cecchinato
The aim of this study was to investigate associations between pathogen-specific cases of subclinical mastitis and milk yield, quality, protein composition, and cheese-making traits. Forty-one multibreed herds were selected for the study, and composite milk samples were collected from 1,508 cows belonging to 3 specialized dairy breeds (Holstein Friesian, Brown Swiss, and Jersey) and 3 dual-purpose breeds of Alpine origin (Simmental, Rendena, and Grey Alpine). Milk composition [i.e., fat, protein, casein, lactose, pH, urea, and somatic cell count (SCC)] was analyzed, and separation of protein fractions was performed by reversed-phase high performance liquid chromatography...
March 29, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28341048/genetic-analysis-of-coagulation-properties-curd-firming-modeling-milk-yield-composition-and-acidity-in-sarda-dairy-sheep
#15
G Bittante, C Cipolat-Gotet, M Pazzola, M L Dettori, G M Vacca, A Cecchinato
Sheep milk is an important source of food, especially in Mediterranean countries, and is used in large part for cheese production. Milk technological traits are important for the sheep dairy industry, but research is lacking into the genetic variation of such traits. Therefore the aim of this study was to estimate the heritability of traditional milk coagulation properties and curd firmness modeled on time t (CFt) parameters, and their genetic relationships with test-day milk yield, composition (fat, protein, and casein content), and acidity in Sarda dairy sheep...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28333549/ksr-as-a-therapeutic-target-for-ras-dependent-cancers
#16
REVIEW
Beth K Neilsen, Danielle E Frodyma, Robert E Lewis, Kurt W Fisher
Targeting downstream effectors required for oncogenic Ras signaling is a potential alternative or complement to the development of more direct approaches targeting Ras in the treatment of Ras-dependent cancers. Areas covered: Here we review literature pertaining to the molecular scaffold Kinase Suppressor of Ras (KSR) and its role in promoting signals critical to tumor maintenance. We summarize the phenotypes in knockout models, describe the role of KSR in cancer, and outline the structure and function of the KSR1 and KSR2 proteins...
May 2017: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/28176872/genotoxic-effects-of-culture-media-on-human-pluripotent-stem-cells
#17
Megha Prakash Bangalore, Syama Adhikarla, Odity Mukherjee, Mitradas M Panicker
Culture conditions play an important role in regulating the genomic integrity of Human Pluripotent Stem Cells (HPSCs). We report that HPSCs cultured in Essential 8 (E8) and mTeSR, two widely used media for feeder-free culturing of HPSCs, had many fold higher levels of ROS and higher mitochondrial potential than cells cultured in Knockout Serum Replacement containing media (KSR). HPSCs also exhibited increased levels of 8-hydroxyguanosine, phospho-histone-H2a.X and p53, as well as increased sensitivity to γ-irradiation in these two media...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069069/arresting-kinase-suppressor-of-ras-in-an-inactive-state
#18
EDITORIAL
Syed Lal Badshah, Yahia Mabkhot
Ras protein signaling pathways are important in controlling the plight of different types of cancer. Here we discussed the paper entitled "Small molecule stabilization of the KSR inactive state antagonizes oncogenic Ras signalling" published in Nature journal on inactivating the kinase suppressor of Ras (KSR) protein using a small molecule as an inhibitor by Dhawan et al. A biphenyl ether analogue of a quinazoline binds in one of the binding pockets of KSR and results in stabilization of its inactive state...
January 9, 2017: Chinese Journal of Cancer
https://www.readbyqxmd.com/read/28003362/phosphorylation-of-rap1-by-camp-dependent-protein-kinase-pka-creates-a-binding-site-for-ksr-to-sustain-erk-activation-by-camp
#19
Maho Takahashi, Yanping Li, Tara J Dillon, Philip J S Stork
Cyclic adenosine monophosphate (cAMP) is an important mediator of hormonal stimulation of cell growth and differentiation through its activation of the extracellular signal-regulated kinase (ERK) cascade. Two small G proteins, Ras and Rap1 have been proposed to mediate this activation. Using HEK293 cells as a model system, we have recently shown that both Ras and Rap1 are required for cAMP signaling to ERKs. However, cAMP-dependent Ras signaling to ERKs is transient and rapidly terminated by PKA phosphorylation of the Raf isoforms C-Raf and B-Raf...
January 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27988128/pathway-based-genome-wide-association-analysis-of-milk-coagulation-properties-curd-firmness-cheese-yield-and-curd-nutrient-recovery-in-dairy-cattle
#20
C Dadousis, S Pegolo, G J M Rosa, D Gianola, G Bittante, A Cecchinato
It is becoming common to complement genome-wide association studies (GWAS) with gene-set enrichment analysis to deepen the understanding of the biological pathways affecting quantitative traits. Our objective was to conduct a gene ontology and pathway-based analysis to identify possible biological mechanisms involved in the regulation of bovine milk technological traits: coagulation properties, curd firmness modeling, individual cheese yield (CY), and milk nutrient recovery into the curd (REC) or whey loss traits...
February 2017: Journal of Dairy Science
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