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https://www.readbyqxmd.com/read/29777692/an-optimal-serum-free-defined-condition-for-in-vitro-culture-of-kidney-organoids
#1
Masaki Nishikawa, Hiroshi Kimura, Naomi Yanagawa, Morgan Hamon, Peter Hauser, Lifu Zhao, Oak D Jo, Norimoto Yanagawa
Kidney organoid is an emerging topic of importance for research in kidney development and regeneration. Conventional culture systems for kidney organoids reported thus far use culture media containing serum, which may compromise our understanding and the potential clinical applicability of the organoid system. In our present study, we tested two serum-free culture conditions and compared their suitability for the maintenance and growth of kidney organoids in culture. One of the serum-free culture conditions was the combination of keratinocytes serum free medium (KSFM) with knockout serum replacement (KSR) (KSFM + KSR), and the other was the combination of knockout DMEM/F12 (KD/F12) and KSR (KD/F12 + KSR)...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29766032/serum-free-erythroid-differentiation-for-efficient-genetic-modification-and-high-level-adult-hemoglobin-production
#2
Naoya Uchida, Selami Demirci, Juan J Haro-Mora, Atsushi Fujita, Lydia N Raines, Matthew M Hsieh, John F Tisdale
In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal hemoglobin production. In this study, we sought to improve both genetic modification and hemoglobin production among human erythroid cells in vitro . To model therapeutic strategies, we transduced human CD34+ cells and peripheral blood mononuclear cells (PBMCs) with lentiviral vectors and compared erythropoietin-based erythroid differentiation using fetal-bovine-serum-containing media and serum-free media...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29680652/technical-note-improving-modeling-of-coagulation-curd-firming-and-syneresis-of-sheep-milk
#3
Claudio Cipolat-Gotet, Michele Pazzola, Alessandro Ferragina, Alessio Cecchinato, Maria L Dettori, Giuseppe M Vacca
The importance of milk coagulation properties for milk processing, cheese yield, and quality is widely recognized. The use of traditional coagulation traits presents several limitations for testing bovine milk and even more for sheep milk, due to its rapid coagulation and curd firming, and early syneresis of coagulum. The aim of this technical note is to test and improve model fitting for assessing coagulation, curd firming, and syneresis of sheep milk. Using milk samples from 87 Sarda ewes, we performed in duplicate lactodynamographic testing...
April 18, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29615470/modulation-of-gq-rho-signaling-by-the-erk-mapk-pathway-controls-locomotion-in-caenorhabditis-elegans
#4
Brantley Coleman, Irini Topalidou, Michael Ailion
The heterotrimeric G protein Gq regulates neuronal activity through distinct downstream effector pathways. In addition to the canonical Gq effector phospholipase Cb, the small GTPase Rho was recently identified as a conserved effector of Gq. To identify additional molecules important for Gq signaling in neurons, we performed a forward genetic screen in the nematode Caenorhabditis elegans for suppressors of the hyperactivity and exaggerated waveform of an activated Gq mutant. We isolated two mutations affecting the MAP kinase scaffold protein KSR-1 and found that KSR-1 modulates locomotion downstream of or in parallel to the Gq-Rho pathway...
April 3, 2018: Genetics
https://www.readbyqxmd.com/read/29596465/homozygous-ksr1-deletion-attenuates-morbidity-but-does-not-prevent-tumor-development-in-a-mouse-model-of-ras-driven-pancreatic-cancer
#5
Elizabeth A Germino, Joseph P Miller, Lauri Diehl, Carter J Swanson, Steffen Durinck, Zora Modrusan, Jeffrey H Miner, Andrey S Shaw
Given the frequency with which MAP kinase signaling is dysregulated in cancer, much effort has been focused on inhibiting RAS signaling for therapeutic benefit. KSR1, a pseudokinase that interacts with RAF, is a potential target; it was originally cloned in screens for suppressors of constitutively active RAS, and its deletion prevents RAS-mediated transformation of mouse embryonic fibroblasts. In this work, we used a genetically engineered mouse model of pancreatic cancer to assess whether KSR1 deletion would influence tumor development in the setting of oncogenic RAS...
2018: PloS One
https://www.readbyqxmd.com/read/29520175/asian-radiology-forum-2016-for-promoting-radiology-together-in-the-asian-oceanian-region-roles-of-the-asian-oceanian-society-of-radiology-and-its-member-societies
#6
EDITORIAL
Ho Sung Kim, Jung-Ah Choi, Jongmin Lee
According to the reports presented at the second Asian Radiology Forum (ARF) 2016, organized by the Korean Society of Radiology (KSR) during the Korean Congress of Radiology (KCR) in September 2016 in Seoul, there is an increasing need for the Asian-Oceanian Society of Radiology (AOSR) and its member societies to promote radiology together in the Asian-Oceanian region. In ARF 2016, the national delegates of the Asian-Oceanian radiological partner societies primarily discussed their societies' activities and contributions in international and regional societies including AOSR, expectations for AOSR, recommendations and suggestions for AOSR, and their societies' support of AOSR...
March 2018: Korean Journal of Radiology: Official Journal of the Korean Radiological Society
https://www.readbyqxmd.com/read/29475885/mek-binding-to-ksr-promotes-allosteric-activation-of-braf
#7
(no author information available yet)
KSR-MEK complexes allosterically activate BRAF, which then phosphorylates a second MEK molecule.
April 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29448017/fetal-bovine-serum-free-cryopreservation-methods-for-clinical-banking-of-human-adipose-derived-stem-cells
#8
Seah Park, Dong Ryul Lee, Ji Sun Nam, Chul Woo Ahn, Haekwon Kim
The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. Here, we investigated if human serum albumin (HSA), human serum (HS), or knockout serum replacement (KSR) can replace FBS for the cryopreservation of MSCs. In addition, we examined the characteristics of MSCs after multiple rounds of cryopreservation. Human adipose-derived stem cells (ASCs) cryopreserved with three FBS replacements, 9% HSA, 90% HS, or 90% KSR, in combination with 10% dimethyl sulfoxide (Me2 SO) maintained stem cell properties including growth, immunophenotypes, gene expression patterns, and the potential to differentiate into adipogenic, osteogenic, and chondrogenic lineages, similar to ASCs frozen with FBS...
April 2018: Cryobiology
https://www.readbyqxmd.com/read/29433126/mek-drives-braf-activation-through-allosteric-control-of-ksr-proteins
#9
Hugo Lavoie, Malha Sahmi, Pierre Maisonneuve, Sara A Marullo, Neroshan Thevakumaran, Ting Jin, Igor Kurinov, Frank Sicheri, Marc Therrien
RAF family kinases have prominent roles in cancer. Their activation is dependent on dimerization of their kinase domains, which has emerged as a hindrance for drug development. In mammals, RAF family kinases include three catalytically competent enzymes (ARAF, BRAF and CRAF) and two pseudokinases (KSR1 and KSR2) that have been described as scaffolds owing to their apparent ability to bridge RAF isoforms and their substrate, mitogen-activated protein kinase kinase (MEK). Kinase suppressor of Ras (KSR) pseudokinases were also shown to dimerize with kinase-competent RAFs to stimulate catalysis allosterically...
February 22, 2018: Nature
https://www.readbyqxmd.com/read/29432471/in-vitro-mouse-spermatogenesis-with-an-organ-culture-method-in-chemically-defined-medium
#10
Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither of which were defined chemically. In this study, we formulated a chemically defined medium (CDM) that can induce mouse spermatogenesis under organ culture conditions. It was found that bovine serum albumin (BSA) purified through three different procedures had different effects on spermatogenesis...
2018: PloS One
https://www.readbyqxmd.com/read/29414992/can-we-induce-spermatogenesis-in-the-domestic-cat-using-an-in-vitro-tissue-culture-approach
#11
Andreia F Silva, Sara Escada-Rebelo, Sandra Amaral, Renata S Tavares, Stefan Schlatt, João Ramalho-Santos, Paula C Mota
The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an alternative mechanism to produce sperm in vitro from immature tissue. However, extension of this technology to other species has remained limited. We have used the domestic cat (Felis catus) as a model for wild felids to investigate spermatogenesis initiation and regulation, with the mouse serving as a control species...
2018: PloS One
https://www.readbyqxmd.com/read/29356242/preferable-in-vitro-condition-for-maintaining-faithful-dna-methylation-imprinting-in-mouse-embryonic-stem-cells
#12
Jiyoung Lee, Ayumi Matsuzawa, Hirosuke Shiura, Akito Sutani, Fumitoshi Ishino
Epigenetic properties of cultured embryonic stem cells (ESCs), including DNA methylation imprinting, are important because they affect the developmental potential. Here, we tested a variety of culture media, including knockout serum replacement (KSR) and fetal bovine serum (FBS) with or without inhibitors of Gsk3β and Mek1/2 (2i) at various time points. In addition to the previously known passage-dependent global changes, unexpected dynamic DNA methylation changes occurred in both maternal and paternal differentially methylated regions: under the widely used condition of KSR with 2i, a highly hypomethylated state occurred at early passages (P1-7) as well as P10, but DNA methylation increased over further passages in most conditions, except under KSR with 2i at P25...
March 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29345310/a-potential-role-of-knockout-serum-replacement-as-a-porcine-follicular-fluid-substitute-for-in-vitro-maturation-lipid-metabolism-approach
#13
Jun-Xue Jin, Sanghoon Lee, Erif Maha Nugraha Setyawan, Anukul Taweechaipaisankul, Geon A Kim, Ho Jae Han, Curie Ahn, Byeong Chun Lee
The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. Knockout serum replacement (KSR) is a synthetic protein source, without any undefined growth factors or differentiation-promoting factors. Therefore, it is feasible to use KSR as a defined component for avoiding effects of unknown molecules in an IVM system...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29344794/ticagrelor-for-secondary-prevention-of-atherothrombotic-events-after-myocardial-infarction-an-evidence-review-group-perspective-of-a-nice-single-technology-appraisal
#14
REVIEW
Xavier G L V Pouwels, Robert Wolff, Bram L T Ramaekers, Anoukh Van Giessen, Shona Lang, Steve Ryder, Gill Worthy, Steven Duffy, Nigel Armstrong, Jos Kleijnen, Manuela A Joore
The National Institute for Health and Care Excellence (NICE) invited AstraZeneca, the manufacturer of ticagrelor (Brilique® ), to submit evidence on the clinical and cost effectiveness of ticagrelor 60 mg twice daily (BID) in combination with low-dose aspirin [acetylsalicylic acid (ASA)] compared with ASA only for secondary prevention of atherothrombotic events in patients with a history of myocardial infarction (MI) and who are at increased risk of atherothrombotic events. Kleijnen Systematic Reviews Ltd (KSR), in collaboration with Maastricht University Medical Centre+, was commissioned as the evidence review group (ERG)...
January 18, 2018: PharmacoEconomics
https://www.readbyqxmd.com/read/29177842/trifluridine-tipiracil-for-previously-treated-metastatic-colorectal-cancer-an-evidence-review-group-perspective-of-a-nice-single-technology-appraisal
#15
REVIEW
Bram L T Ramaekers, Robert Wolff, Anoukh van Giessen, Xavier Pouwels, Debra Fayter, Shona Lang, Nigel Armstrong, Gill Worthy, Steven Duffy, Jos Kleijnen, Manuela A Joore
The National Institute for Health and Care Excellence (NICE) invited Servier, the company manufacturing trifluridine and tipiracil (T/T; trade name: Lonsurf® ), to submit evidence for the clinical and cost effectiveness of T/T compared with best supportive care (BSC) for metastatic colorectal cancer (third-line or later). Kleijnen Systematic Reviews Ltd (KSR), in collaboration with Maastricht University Medical Center, was commissioned as the Evidence Review Group (ERG). This paper presents a summary of the company's submission (CS), the ERG report and the development of the NICE guidance for the use of this drug in England and Wales by the appraisal committee (AC)...
March 2018: PharmacoEconomics
https://www.readbyqxmd.com/read/29066538/the-scaffolding-protein-cnk-binds-to-the-receptor-tyrosine-kinase-alk-to-promote-visceral-founder-cell-specification-in-drosophila
#16
Georg Wolfstetter, Kathrin Pfeifer, Jesper R van Dijk, Fredrik Hugosson, Xiangyi Lu, Ruth H Palmer
In Drosophila melanogaster, the receptor tyrosine kinase (RTK) anaplastic lymphoma kinase (Alk) and its ligand jelly belly (Jeb) are required to specify muscle founder cells in the visceral mesoderm. We identified a critical role for the scaffolding protein Cnk (connector enhancer of kinase suppressor of Ras) in this signaling pathway. Embryos that ectopically expressed the minimal Alk interaction region in the carboxyl terminus of Cnk or lacked maternal and zygotic cnk did not generate visceral founder cells or a functional gut musculature, phenotypes that resemble those of jeb and Alk mutants...
October 24, 2017: Science Signaling
https://www.readbyqxmd.com/read/29026529/coordinating-erk-signaling-via-the-molecular-scaffold-kinase-suppressor-of-ras
#17
REVIEW
Danielle Frodyma, Beth Neilsen, Diane Costanzo-Garvey, Kurt Fisher, Robert Lewis
Many cancers, including those of the colon, lung, and pancreas, depend upon the signaling pathways induced by mutated and constitutively active Ras. The molecular scaffolds Kinase Suppressor of Ras 1 and 2 (KSR1 and KSR2) play potent roles in promoting Ras-mediated signaling through the Raf/MEK/ERK kinase cascade. Here we summarize the canonical role of KSR in cells, including its central role as a scaffold protein for the Raf/MEK/ERK kinase cascade, its regulation of various cellular pathways mediated through different binding partners, and the phenotypic consequences of KSR1 or KSR2 genetic inactivation...
2017: F1000Research
https://www.readbyqxmd.com/read/28925143/-exploration-of-rapidly-determining-quality-of-traditional-chinese-medicines-by-nir-spectroscopy-based-on-internet-sharing-mode
#18
Li-Jun Ni, Shao-Rong Luan, Li-Guo Zhang
Because of the numerous varieties of herbal species and active ingredients in the traditional Chinese medicine(TCM),the traditional methods employed could hardly satisfy the current determination requirements of TCM.The present work proposed an idea to realize rapid determination of the quality of TCM based on near infrared(NIR)spectroscopy and internet sharing mode. Low cost and portable multi-source composite spectrometer was invented by our group for in-site fast measurement of spectra of TCM samples. The database could be set up by sharing spectra and quality detection data of TCM samples among TCM enterprises based on the internet platform...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28840818/polycladomyces-subterraneus-sp-nov-isolated-from-soil-in-thailand
#19
Narisara Maneewong, Chatsuda Sakdapetsiri, Chanwit Suriyachadkun, Chiyo Shibata, Tomohiko Tamura, Shinji Tokuyama, Vichien Kitpreechavanich
A thermophilic poly(L-lactide)-degrading Gram-stain-positive filamentous bacterial strain that develops single spores on the aerial mycelium was isolated from forest soil at Srinagarind Dam, Kanchanaburi Province, Thailand. The results of a polyphasic taxonomic study showed that our isolate had characteristics typical of members of the genus Polycladomyces. The isolate grew aerobically at an optimum temperature of 50-55 °C and optimal pH 6-7. Meso-diaminopimelic acid was present as the diagnostic diamino acid in the peptidoglycan but no characteristic sugars are detected...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28673682/efficient-neural-differentiation-of-mouse-pluripotent-stem-cells-in-a-serum-free-medium-and-development-of-a-novel-strategy-for-enrichment-of-neural-cells
#20
Isha Verma, Zubin Rashid, Sujit K Sikdar, Polani B Seshagiri
Pluripotent stem cells (PSCs) offer an excellent model to study neural development and function. Although various protocols have been developed to direct the differentiation of PSCs into desired neural cell types, many of them suffer from limitations including low efficiency, long duration of culture, and the use of expensive, labile, and undefined growth supplements. In this study, we achieved efficient differentiation of mouse PSCs to neural lineage, in the absence of exogenous molecules, by employing a serum-free culture medium containing knockout serum replacement (KSR)...
July 1, 2017: International Journal of Developmental Neuroscience
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