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https://www.readbyqxmd.com/read/28820955/integration-of-an-in-situ-maldi-based-high-throughput-screening-process-a-case-study-with-receptor-tyrosine-kinase-c-met
#1
Katrin Beeman, Jens Baumgärtner, Manuel Laubenheimer, Karlheinz Hergesell, Martin Hoffmann, Ulrich Pehl, Frank Fischer, Jan-Carsten Pieck
Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated "in-line reader" for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components...
August 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28820460/determination-of-sphingosine-1-phosphate-in-human-plasma-using-liquid-chromatography-coupled-with-q-tof-mass-spectrometry
#2
Emmanuel Eroume-A Egom, Ross Fitzgerald, Rebecca Canning, Rebabonye B Pharithi, Colin Murphy, Vincent Maher
Evidence suggests that high-density lipoprotein (HDL) components distinct from cholesterol, such as sphingosine-1-phosphate (S1P), may account for the anti-atherothrombotic effects attributed to this lipoprotein. The current method for the determination of plasma levels of S1P as well as levels associated with HDL particles is still cumbersome an assay method to be worldwide practical. Recently, a simplified protocol based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the sensitive and specific quantification of plasma levels of S1P with good accuracy has been reported...
August 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28820379/conveyance-contact-investigation-for-imported-middle-east-respiratory-syndrome-cases-united-states-may-2014
#3
Susan A Lippold, Tina Objio, Laura Vonnahme, Faith Washburn, Nicole J Cohen, Tai-Ho Chen, Paul J Edelson, Reena Gulati, Christa Hale, Jennifer Harcourt, Lia Haynes, Amy Jewett, Robynne Jungerman, Katrin S Kohl, Congrong Miao, Nicolette Pesik, Joanna J Regan, Efrosini Roland, Chris Schembri, Eileen Schneider, Azaibi Tamin, Kathleen Tatti, Francisco Alvarado-Ramy
In 2014, the Centers for Disease Control and Prevention conducted conveyance contact investigations for 2 Middle East respiratory syndrome cases imported into the United States, comprising all passengers and crew on 4 international and domestic flights and 1 bus. Of 655 contacts, 78% were interviewed; 33% had serologic testing. No secondary cases were identified.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28819602/fracture-dislocation-of-shoulder-with-brachial-plexus-palsy-a-case-report-and-review-of-management-options
#4
Sameer Rathore, Srinivas Kasha, Srinivas Yeggana
INTRODUCTION: Injuries causing fracture dislocation of shoulder and brachial plexus palsy are extremely rare. As per authors' knowledge, three part fracture of proximal humerus with shoulder dislocation and brachial plexus palsy has not been reported in the literature. CASE PRESENTATION: A 53 year old female sustained a three part fracture of right proximal humerus along with dislocation of shoulder joint and brachial plexus palsy following a fall from a flight of stairs...
March 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28818772/multipath-ultrasonic-gas-flow-meter-based-on-multiple-reference-waves
#5
Hongliang Zhou, Tao Ji, Ruichen Wang, Xiaocheng Ge, Xiaoyu Tang, Shizhen Tang
Several technologies can be used in ultrasonic gas flow-meters, such as transit-time, Doppler, cross-correlation and etc. In applications, the approach based on measuring transit-time has demonstrated its advantages and become more popular. Among those techniques which can be applied to determine time-of-flight (TOF) of ultrasonic waves, including threshold detection, cross correlation algorithm and other digital signal processing algorithms, cross correlation algorithm has more advantages when the received ultrasonic signal is severely disturbed by the noise...
July 18, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28818663/a-lightweight-inexpensive-robotic-system-for-insect-vision
#6
Chelsea Sabo, Robert Chisholm, Adam Petterson, Alex Cope
Designing hardware for miniaturized robotics which mimics the capabilities of flying insects is of interest, because they share similar constraints (i.e. small size, low weight, and low energy consumption). Research in this area aims to enable robots with similarly efficient flight and cognitive abilities. Visual processing is important to flying insects' impressive flight capabilities, but currently, embodiment of insect-like visual systems is limited by the hardware systems available. Suitable hardware is either prohibitively expensive, difficult to reproduce, cannot accurately simulate insect vision characteristics, and/or is too heavy for small robotic platforms...
August 14, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/28818152/dr-william-carpentier-apollo-11-flight-surgeon
#7
Mark R Campbell
No abstract text is available yet for this article.
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818151/you-re-the-flight-surgeon
#8
(no author information available yet)
Hatcher DR. You're the flight surgeon: migraine aura without headaches. Aerosp Med Hum Perform. 2017; 88(9):887-891.
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818150/you-re-the-flight-surgeon
#9
(no author information available yet)
Casstevens EA. You're the flight surgeon: an unexpected twist. Aerosp Med Hum Perform. 2017; 88(9):884-887.
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818149/cerebellar-infarction-presenting-with-acute-vestibular-syndrome-in-two-u-s-air-force-pilots
#10
Roger R Hesselbrock
BACKGROUND: Cerebellar infarction is an uncommon but serious cause of isolated acute vestibular symptoms, particularly in young, healthy individuals, and can easily be overlooked. We present two cases of cerebellar infarction in U.S. Air Force pilots, one of which occurred during flight. CASE REPORTS: A 41-yr-old man developed acute vertigo, disequilibrium, nausea, and headache, with progressive slow symptomatic improvement, and presented to medical attention 4 d after symptom onset...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818148/cross-sectional-survey-of-physicians-on-providing-volunteer-care-for-in-flight-medical-events
#11
Eric Chatfield, William F Bond, Bradley McCay, Claude Thibeault, Paulo M Alves, Marc Squillante, Joshua Timpe, Courtney J Cook, Raymond E Bertino
BACKGROUND: Airline carriers have equipment, procedures, and protocols in place to handle in-flight medical events (IFMEs). Community physicians may be asked for aid during IFMEs. Cross-Sectional Survey of Physicians on Providing Volunteer Care for In-Flight Medical Events surveyed self-assessed awareness and knowledge, perceived barriers, and suggestions for improving responses to IFMEs. METHODS: We composed a survey regarding clinicians' self-assessed understanding of in-flight resources, procedures, flight environmental issues, and Good Samaritan protections...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818147/attention-deficit-hyperactivity-disorder-and-fatal-accidents-in-aviation-medicine
#12
Tanja Laukkala, Robert Bor, Bruce Budowle, Antti Sajantila, Pooshan Navathe, Markku Sainio, Alpo Vuorio
BACKGROUND: Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder with symptoms of inattention and/or hyperactivity-impulsivity that interfere with functioning and/or development. ADHD occurs in about 2.5% of adults. ADHD can be an excluding medical condition among pilots due to the risk of attentional degradation and therefore impact on flight safety. Diagnosis of ADHD is complex, which complicates aeromedical assessment. This study highlights fatal accident cases among pilots with ADHD and discusses protocols to detect its presence to help to assess its importance to flight safety...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818145/protective-effects-of-emotional-intelligence-and-proactive-coping-on-civil-pilots-mental-health
#13
Yaning Guo, Ming Ji, Xuqun You, Jie Huang
INTRODUCTION: Pilots' mental health is an issue of crucial importance that may endanger flight safety. As such, it is of profound significance to address the question of what characteristics are protective of mental health among pilots. The present study aimed to explore the indirect effects of emotional intelligence (EI) on depression and anxiety via proactive coping, and examine the moderating role of previous flight experience (PFE) in the conditional indirect effect of EI on depression through proactive coping...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818143/locomotion-strategy-and-magnitude-of-ground-reaction-forces-during-treadmill-training-on-iss
#14
Elena Fomina, Alexandra Savinkina
INTRODUCTION: Creation of the cosmonaut in-flight physical training process is currently based on the leading role of support afferents in the development of hypogravity changes in the motor system. We assume that the strength of support afferents is related to the magnitude of the ground reaction forces (GRF). For this purpose it was necessary to compare the GRF magnitude on the Russian BD-2 treadmill for different locomotion types (walking and running), modes (active and passive), and subjects...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28818142/psychophysiological-assessment-in-pilots-performing-challenging-simulated-and-real-flight-maneuvers
#15
Bernd Johannes, Stefanie Rothe, André Gens, Soeren Westphal, Katja Birkenfeld, Edwin Mulder, Jörn Rittweger, Carla Ledderhos
BACKGROUND: The objective assessment of psychophysiological arousal during challenging flight maneuvers is of great interest to aerospace medicine, but remains a challenging task. In the study presented here, a vector-methodological approach was used which integrates different psychophysiological variables, yielding an integral arousal index called the Psychophysiological Arousal Value (PAV). METHODS: The arousal levels of 15 male pilots were assessed during predetermined, well-defined flight maneuvers performed under simulated and real flight conditions...
September 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28817985/rapid-characterisation-and-identification-of-compounds-in-saposhnikoviae-radix-by-high-performance-liquid-chromatography-coupled-with-electrospray-ionisation-quadrupole-time-of-flight-mass-spectrometry
#16
Luxiao Chen, Xiangyang Chen, Lei Su, Yanyan Jiang, Bin Liu
Saposhnikoviae Radix (SR), the dried root of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae), is commonly used as a traditional Chinese medicine. In this study, a rapid and accurate method was firstly, developed for the qualitative analysis of SR by high-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q-TOF-MS/MS). A total of 45 compounds were identified or tentatively characterised, including 13 chromones, 28 coumarins and four others...
August 18, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28817712/study-of-the-cross-market-effects-of-brexit-based-on-the-improved-symbolic-transfer-entropy-garch-model-an-empirical-analysis-of-stock-bond-correlations
#17
Xiurong Chen, Yixiang Tian, Rubo Zhao
In this paper, we study the cross-market effects of Brexit on the stock and bond markets of nine major countries in the world. By incorporating information theory, we introduce the time-varying impact weights based on symbolic transfer entropy to improve the traditional GARCH model. The empirical results show that under the influence of Brexit, flight-to-quality not only commonly occurs between the stocks and bonds of each country but also simultaneously occurs among different countries. We also find that the accuracy of the time-varying symbolic transfer entropy GARCH model proposed in this paper has been improved compared to the traditional GARCH model, which indicates that it has a certain practical application value...
2017: PloS One
https://www.readbyqxmd.com/read/28817631/a-computational-model-for-biosonar-echoes-from-foliage
#18
Chen Ming, Anupam Kumar Gupta, Ruijin Lu, Hongxiao Zhu, Rolf Müller
Since many bat species thrive in densely vegetated habitats, echoes from foliage are likely to be of prime importance to the animals' sensory ecology, be it as clutter that masks prey echoes or as sources of information about the environment. To better understand the characteristics of foliage echoes, a new model for the process that generates these signals has been developed. This model takes leaf size and orientation into account by representing the leaves as circular disks of varying diameter. The two added leaf parameters are of potential importance to the sensory ecology of bats, e...
2017: PloS One
https://www.readbyqxmd.com/read/28817629/maldi-tof-ms-identification-of-anopheles-gambiae-giles-blood-meal-crushed-on-whatman-filter-papers
#19
Sirama Niare, Lionel Almeras, Fatalmoudou Tandina, Amina Yssouf, Affane Bacar, Ali Toilibou, Ogobara Doumbo, Didier Raoult, Philippe Parola
BACKGROUND: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals...
2017: PloS One
https://www.readbyqxmd.com/read/28816383/the-structure-and-ultrastructure-of-the-egg-capsules-of-stoneflies-of-the-genus-isoperla-insecta-plecoptera-perlodidae
#20
Anna Michalik, Marko Miliša, Katarzyna Michalik, Elżbieta Rościszewska
The egg capsules of five systellognathan stoneflies species representing the genus Isoperla (Plecoptera, Perlodidae) have been investigated using light and electron microscopes (SEM and TEM). We consider the structural modifications of egg coverings (egg capsules, eggshells) like: a shape of a capsule, presence of structures fixing an egg to the substratum under water, intrachorionic aeropylar system facilitating gas exchange as a factor adapting an egg/embryo to the environment. The structures protecting eggs against desiccation during a female flight before oviposition into water as well as against the dangers of external mechanical injury caused by turbulences in rapid water currents of mountain streams are described and discussed...
August 17, 2017: Microscopy Research and Technique
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