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https://www.readbyqxmd.com/read/28336812/tgf-%C3%AE-1-stimulates-hdac4-nucleus-to-cytoplasm-translocation-and-nadph-oxidase4-derived-reactive-oxygen-species-in-normal-human-lung-fibroblasts
#1
Weichao Guo, Shigeki Saito, Cecilia G Sanchez, Yan Zhuang, Rafael E Gongora Rosero, Bin Shan, Fayong Luo, Joseph A Lasky
Myofibroblasts are important mediators of fibrogenesis, thus blocking fibroblast to myofibroblast differentiation (FMD) may be an effective strategy to treat pulmonary fibrosis (PF). Previously we reported that HDAC4 activity is necessary for TGF-β1-induced human lung FMD. Here we show that TGF-β1 increases NOX4 mRNA and protein expression in normal human lung fibroblasts (NHLFs) and causes nuclear export of HDAC4. Application of the NOX family inhibitor, diphenyleneiodonium chloride (DPI), reduces TGF-β1-induced HDAC4 nuclear export, expression of the myofibroblast marker α-smooth muscle actin (α-SMA), and α-SMA fiber formation...
March 23, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28335679/puerarin-attenuates-renal-fibrosis-by-reducing-oxidative-stress-induced-epithelial-cell-apoptosis-via-mapk-signal-pathways-in-vivo-and-in-vitro
#2
Xiangjun Zhou, Chen Bai, Xinbo Sun, Xiaoxin Gong, Yong Yang, Congbo Chen, Guang Shan, Qisheng Yao
Puerarin (PR) is an isoflavonoid isolated from the root of the plant Pueraria lobata and has been widely used in traditional Chinese herbal medicine for the treatment of various diseases. Oxidative stress and epithelial cell apoptosis play important roles in the renal fibrotic process. The present study aimed to determine whether or not PR inhibits renal fibrosis by reducing oxidative stress induced-epithelial cell apoptosis. In vivo, unilateral ureteral obstruction (UUO) induced renal fibrosis, and epithelial cell apoptosis...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28330417/involvement-of-nox2-and-nox4-nadph-oxidases-in-early-brain-injury-after-subarachnoid-hemorrhage
#3
Li Zhang, Zhen Li, Dongxia Feng, Haitao Shen, Xiaodi Tian, Haiying Li, Zhong Wang, Gang Chen
Oxidative stress is responsible for a poor prognosis of subarachnoid hemorrhage (SAH) patients. Nox2 has been shown to participate in SAH-induced early brain injury (EBI). Nox4 is another major subtype of Nox family widely expressed in CNS. Here, we investigated the role of Nox4 and whether there was a synergistic effect of Nox2 and Nox4 in SAH-induced EBI. Clinical brain biopsies of four patients with traumatic brain injury and perihematomal brain tissue from six subjects with SAH were examined. Gp91ds-tat (a specific inhibitor of Nox2), GKT137831 (a specific inhibitor of Nox4), and apocynin (a non-specific Nox inhibitor) were used to test the role of Nox2 and Nox4...
March 22, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28325789/sex-specific-differences-in-mitochondria-biogenesis-morphology-respiratory-function-and-ros-homeostasis-in-young-mouse-heart-and-brain
#4
Abdel Rahman M Khalifa, Engy A Abdel-Rahman, Ali M Mahmoud, Mohamed H Ali, Maha Noureldin, Saber H Saber, Mahmoud Mohsen, Sameh S Ali
Sex-specific differences in mitochondrial function and free radical homeostasis are reported in the context of aging but not well-established in pathogeneses occurring early in life. Here, we examine if sex disparity in mitochondria function, morphology, and redox status starts early and hence can be implicated in sexual dimorphism in cardiac as well as neurological disorders prevalent at young age. Although mitochondrial activity in the heart did not significantly vary between sexes, female brain exhibited enhanced respiration and higher reserve capacity...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28323024/ppar%C3%AE-activation-by-mhy908-attenuates-age-related-renal-inflammation-through-modulation-of-the-ros-akt-foxo1-pathway
#5
Ye Ra Kim, Eun Kyeong Lee, Dae Hyun Kim, Kyung Mok Kim, Bonggi Lee, Hye Jin An, June Whoun Park, Kyoung Mi Moon, Min Hi Park, Ki Wung Chung, Ji Young Park, Seong Jin Kim, Hwi Young Yun, Sujin Son, Pusoon Chun, Hyung Ryong Moon, Hae Young Chung
2-[4-(5-Chlorobenzothiazothiazol-2-yl)phenoxy]-2-methyl-propionic acid (MHY908) has been shown to prevent insulin resistance-induced hyperinsulinemia in aged rats. However, the mechanism underlying MHY908-mediated amelioration of renal inflammation with insulin resistance during aging remains unknown. This study investigated the effects of MHY908 on age-related changes in the IRS/Akt/forkhead box (FoxO) 1 signaling pathway in the kidneys of aged rats and HEK293T cells. Experiments were performed in young, old, and MHY908-fed old rats (1mg or 3mg/kg/day MHY908 for 4 weeks)...
March 18, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28316179/-effects-and-related-mechanism-of-angiotensin-1-7-on-toll-like-receptor-4-mediated-oxidative-stress-in-human-umbilical-vein-endothelial-cells
#6
W F Yan, J J Xue, H Y Yang, B Liang, Z M Yang
Objective: To explore the role and related mechanisms of angiotensin-(1-7)(Ang-(1-7)) on Toll-like receptor 4 (TLR4) mediated oxidized low-density lipoprotein(ox-LDL)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured in vitro and divided into six groups: the control group (normal medium), the ox-LDL group(treated with 75 mg/L ox-LDL), the ox-LDL+ Ang-(1-7) group (1 μmol/L Ang-(1-7) pretreated for 30 minutes, then intervened with 75 mg/L ox-LDL), the ox-LDL+ Ang-(1-7)+ A-779 group(1 μmol/L A-779 (Mas receptor) pretreated for 30 minutes, 1 μmol/L Ang-(1-7) pretreated for 30 minutes, then intervened with 75 mg/L ox-LDL), the ox-LDL+ A-779 group (1 μmol/L A-779 pretreated for 30 minutes, then intervened with 75 mg/L ox-LDL), the ox-LDL+ HTA125 group (10 μg/L HTA125 (TLR4-blocking antibody) pretreated for 30 minutes, then intervened with 75 mg/L ox-LDL )...
March 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28316159/-mechanism-of-angiotensin-converting-enzyme-2-overexpression-improving-collagen-synthesis-in-lung
#7
Q J Yang, B B Zheng, N N Sun, M X Pan, Z M Zheng, Y Meng
Objective: To explore the mechanism of angiotensin-converting enzyme 2 (ACE2) overexpression improving collagen synthesis in lung. Methods: Lung fibroblasts of mice over-expressing ACE2 and the wild type (WT) were cultured in vitro and divided into 5 groups: WT-control, WT-AngiotensinⅡ (AngⅡ), ACE2(+ /+) -control, ACE2(+ /+) -AngⅡ and ACE2(+ /+) -AngⅡ+ A779. The protein relative expression levels of ACE2, collagen Ⅰ, nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3), autophagy-related protein (Beclin1), ubiquitin-binding protein p62 (P62), microtubule-associated proteins light chain 3-Ⅱ (LC3-Ⅱ) were measured by Western blot and triphosadenine (ATP) level was measured by ATP Assay Kit...
March 14, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28314381/dysregulation-of-alveolar-macrophage-ppar%C3%AE-nadph-oxidases-and-tgf%C3%AE-sub-1-sub-in-otherwise-healthy-hiv-infected-individuals
#8
Samantha M Yeligar, Janine M Ward, Frank L Harris, Lou Ann Brown, David Guidot, Sushma K Cribbs
<u>Rationale:</u> Despite antiretroviral therapy (ART), respiratory infections increase mortality in individuals living with chronic human immunodeficiency virus (HIV) infection. In experimental and clinical studies of chronic HIV infection, alveolar macrophages (AMs) exhibit impaired phagocytosis and bacterial clearance. Peroxisome proliferator-activated receptor (PPAR)γ, NADPH oxidase (Nox) isoforms Nox1, Nox2, Nox4, and transforming growth factor-beta 1 (TGFβ<sub>1</sub>) are critical mediators of AM oxidative stress and phagocytic dysfunction...
March 17, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28297659/oncogene-selective-sensitivity-to-synchronous-cell-death-following-modulation-of-the-amino-acid-nutrient-cystine
#9
Ioannis Poursaitidis, Xiaomeng Wang, Thomas Crighton, Christiaan Labuschagne, David Mason, Shira L Cramer, Kendra Triplett, Rajat Roy, Olivier E Pardo, Michael J Seckl, Scott W Rowlinson, Everett Stone, Richard F Lamb
Cancer cells reprogram their metabolism, altering both uptake and utilization of extracellular nutrients. We individually depleted amino acid nutrients from isogenic cells expressing commonly activated oncogenes to identify correspondences between nutrient supply and viability. In HME (human mammary epithelial) cells, deprivation of cystine led to increased cell death in cells expressing an activated epidermal growth factor receptor (EGFR) mutant. Cell death occurred via synchronous ferroptosis, with generation of reactive oxygen species (ROS)...
March 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28292711/co-releasing-molecules-corm2-attenuates-angiotensin-ii-induced-human-aortic-smooth-muscle-cell-migration-through-inhibition-of-ros-il-6-generation-and-matrix-metalloproteinases-9-expression
#10
Ming-Horng Tsai, Chiang-Wen Lee, Lee-Fen Hsu, Shu-Yu Li, Yao-Chang Chiang, Ming-Hsueh Lee, Chun-Han Chen, Hwey-Fang Liang, Jia-Mei How, Pey-Jium Chang, Ching-Mei Wu, I-Ta Lee
Ang II has been involved in the pathogenesis of cardiovascular diseases, and matrix metalloproteinase-9 (MMP-9) induced migration of human aortic smooth muscle cells (HASMCs) is the most common and basic pathological feature. Carbon monoxide (CO), a byproduct of heme breakdown by heme oxygenase, exerts anti-inflammatory effects in various tissues and organ systems. In the present study, we aimed to investigate the effects and underlying mechanisms of carbon monoxide releasing molecule-2 (CORM-2) on Ang II-induced MMP-9 expression and cell migration of HASMCs...
March 1, 2017: Redox Biology
https://www.readbyqxmd.com/read/28260074/atorvastatin-prolongs-the-lifespan-of-radiation%C3%A2-induced-reactive-oxygen-species-in-pc-3-prostate-cancer-cells-to-enhance-the-cell-killing-effect
#11
Hao Yu, Shao-Qian Sun, Xiao-Bin Gu, Wen Wang, Xian-Shu Gao
Studies have reported that atorvastatin (ATO) may increase the radiosensitivity of malignant cells. However, the influence of ATO on reactive oxygen species (ROS) levels before and after irradiation has not been fully illustrated. In the present study, radiosensitivity was evaluated by a clonogenic assay and a cell survival curve and cell apoptosis was measured by flow cytometry. ROS were detected by a laser scanning confocal microscope and flow cytometry with a DCFH-DA probe. NADPH oxidases (NOXs) and superoxide dismutase (SOD) proteins were detected by immunoblotting, and total SOD activity was measured using an SOD kit...
February 14, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28249816/anti-inflammatory-and-antioxidant-effects-of-polyphenols-extracted-from-antirhea-borbonica-medicinal-plant-on-adipocytes-exposed-to-porphyromonas-gingivalis-and-escherichia-coli-lipopolysaccharides
#12
Fanny Le Sage, Olivier Meilhac, Marie-Paule Gonthier
In obesity, gut microbiota LPS may translocate into the blood stream and then contribute to adipose tissue inflammation and oxidative stress, leading to insulin resistance. A causal link between periodontal infection, obesity and type 2 diabetes has also been suggested. We evaluated the ability of polyphenols from Antirhea borbonica medicinal plant to improve the inflammatory and redox status of 3T3-L1 adipocytes exposed to LPS of Porphyromonas gingivalis periodontopathogen or Escherichia coli enterobacteria...
February 27, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28249219/psoriasis-like-inflammation-leads-to-renal-dysfunction-via-upregulation-of-nadph-oxidases-and-inducible-nitric-oxide-synthase
#13
Naif O Al-Harbi, Ahmed Nadeem, Mushtaq A Ansari, Mohammed M Al-Harbi, Moureq R Alotaibi, Abdulaziz M S AlSaad, Sheikh F Ahmad
Psoriatic patients have systemic inflammation as well as oxidative stress, which are associated with cardiovascular disorders such as atherosclerosis, hypertension myocardial infarction, and stroke. Psoriasis has also been shown to be associated with kidney disease in several studies. Both disorders also have strong component of oxidative stress which usually emanates from NADPH oxidases (NOXs) and inducible nitric oxide synthase (iNOS). However, whether psoriatic inflammation leads to renal oxidative stress and dysfunction remains unexplored...
February 26, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28246402/grape-seed-proanthocyanidins-prevent-irradiation-induced-differentiation-of-human-lung-fibroblasts-by-ameliorating-mitochondrial-dysfunction
#14
XiaoHong Yang, Tao Liu, Bo Chen, Fangqin Wang, Qunfang Yang, XiaoHong Chen
Radiation-induced lung fibrosis (RILF) is a long-term adverse effect of curative radiotherapy. The accumulation of myofibroblasts in fibroblastic foci is a pivotal feature of RILF. In the study, we found the inhibitory effect of grape seed proanthocyanidins (GSPs) on irradiation-induced differentiation of human fetal lung fibroblasts (HFL1). To explore the mechanism by which GSPs inhibit fibroblast differentiation, we measured the reactive oxygen species (ROS) levels, mitochondrial function, mitochondrial dynamics, glycolysis and the signaling molecules involved in fibroblast transdifferentiation...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28230797/zyz-772-prevents-cardiomyocyte-injury-by-suppressing-nox4-derived-ros-production-and-apoptosis
#15
Ying Wang, Liangjie Zhong, Xinhua Liu, Yi Zhun Zhu
Nox-dependent signaling plays critical roles in the development of heart failure, cardiac hypertrophy, and myocardial infarction. NADPH oxidase 4 (Nox4) as a major source of oxidative stress in the heart offers a new therapeutic target in cardiovascular disease. In the present work, a novel flavonoid was isolated from Zanthoxylum bungeanum. Its structure was elucidated as Quercetin-3-O-(6''-O-α-l-rhamnopyransoyl)-β-d-glucopyranoside-7-O-β-d-glucopyranoside (ZYZ-772) for the first time. ZYZ-772 exhibited significant cardio-protective property against CoCl₂ induced H9c2 cardiomyocyte cells injury...
February 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28228756/agonistic-anti-pdgf-receptor-autoantibodies-from-patients-with-systemic-sclerosis-impact-human-pulmonary-artery-smooth-muscle-cells-function-in-vitro
#16
Silvia Svegliati, Donatella Amico, Tatiana Spadoni, Colomba Fischetti, Doreen Finke, Gianluca Moroncini, Chiara Paolini, Cecilia Tonnini, Antonella Grieco, Marina Rovinelli, Armando Gabrielli
One of the earliest events in the pathogenesis of systemic sclerosis (SSc) is microvasculature damage with intimal hyperplasia and accumulation of cells expressing PDGF receptor. Stimulatory autoantibodies targeting PDGF receptor have been detected in SSc patients and demonstrated to induce fibrosis in vivo and convert in vitro normal fibroblasts into SSc-like cells. Since there is no evidence of the role of anti-PDGF receptor autoantibodies in the pathogenesis of SSc vascular lesions, we investigated the biologic effect of agonistic anti-PDGF receptor autoantibodies from SSc patients on human pulmonary artery smooth muscle cells and the signaling pathways involved...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28225655/mnte-2-pyp-treatment-or-nox4-inhibition-protects-against-radiation-induced-damage-in-mouse-primary-prostate-fibroblasts-by-inhibiting-the-tgf-beta-1-signaling-pathway
#17
Arpita Chatterjee, Elizabeth A Kosmacek, Rebecca E Oberley-Deegan
Prostate cancer patients who undergo radiotherapy frequently suffer from side effects caused by radiation-induced damage to normal tissues adjacent to the tumor. Exposure of these normal cells during radiation treatment can result in tissue fibrosis and cellular senescence, which ultimately leads to postirradiation-related chronic complications including urinary urgency and frequency, erectile dysfunction, urethral stricture and incontinence. Radiation-induced reactive oxygen species (ROS) have been reported as the most potent causative factor for radiation damage to normal tissue...
February 22, 2017: Radiation Research
https://www.readbyqxmd.com/read/28225041/pvn-blockade-of-p44-42-mapk-pathway-attenuates-salt-induced-hypertension-through-modulating-neurotransmitters-and-attenuating-oxidative-stress
#18
Hong-Li Gao, Xiao-Jing Yu, Kai-Li Liu, Xiao-Lian Shi, Jie Qi, Yan-Mei Chen, Yan Zhang, Juan Bai, Qiu-Yue Yi, Zhi-Peng Feng, Wen-Sheng Chen, Wei Cui, Jin-Jun Liu, Guo-Qing Zhu, Yu-Ming Kang
The imbalance of neurotransmitters and excessive oxidative stress responses contribute to the pathogenesis of hypertension. In this study, we determined whether blockade of p44/42 MAPK pathway in the hypothalamic paraventricular nucleus (PVN) ameliorates the development of hypertension through modulating neurotransmitters and attenuating oxidative stress. Dahl salt-sensitive (S) rats received a high-salt diet (HS, 8% NaCl) or a normal-salt diet (NS, 0.3% NaCl) for 6 weeks and were treated with bilateral PVN infusion of PD-98059 (0...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216438/porphyromonas-gingivalis-lipopolysaccharide-induces-pro-inflammatory-adipokine-secretion-and-oxidative-stress-by-regulating-toll-like-receptor-mediated-signaling-pathways-and-redox-enzymes-in-adipocytes
#19
Fanny Le Sage, Olivier Meilhac, Marie-Paule Gonthier
Gut microbiota LPS contributes to obesity-related chronic inflammation and oxidative stress, promoting insulin resistance. Periodontal disease also represents a risk factor for type 2 diabetes and is associated with obesity. This study compared the effect of LPS from P. gingivalis periodontopathogen and E. coli enterobacteria on inflammatory adipokine secretion and redox status of 3T3-L1 adipocytes. We found that both LPS activated TLR2- and TLR4-mediated signaling pathways involving MyD88 adaptor and NFκB transcription factor, leading to an increased secretion of leptin, resistin, IL-6 and MCP-1...
February 20, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28211114/magnesium-lithospermate-b-from-salvia-miltiorrhiza-bunge-ameliorates-aging-induced-renal-inflammation-and-senescence-via-nadph-oxidase-mediated-reactive-oxygen-generation
#20
Chan Hum Park, Sung Ho Shin, Eun Kyeong Lee, Dae Hyun Kim, Min-Jo Kim, Seong-Soo Roh, Takako Yokozawa, Hae Young Chung
The present study was conducted to examine whether magnesium lithospermate B (MLB) extracted from Salviae miltiorrhizae radix was renoprotective in pathways related to age-related oxidative stress in aged rats. Magnesium lithospermate B was orally administered at a dose of 2- or 8-mg/kg body weight for 16 consecutive days, and the effects were compared with those of vehicle in old and young rats. Magnesium lithospermate B administration to old rats ameliorated renal oxidative stress through reduction of reactive oxygen species...
February 16, 2017: Phytotherapy Research: PTR
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