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https://www.readbyqxmd.com/read/29332283/markers-of-developmentally-regulated-programmed-cell-death-and-their-analysis-in-cereal-seeds
#1
Fernando Domínguez, Francisco Javier Cejudo
Programmed cell death (PCD) is a key process for the development and differentiation of multicellular organisms, which is characterized by well-defined morphological and biochemical features. These include chromatin condensation, DNA degradation and nuclear fragmentation, with nucleases and proteases playing a relevant function in these processes. In this chapter we describe methods routinely used for the analysis of hallmarks of developmentally regulated PCD in cereal seed tissues, which are based on agarose and polyacrylamide gel electrophoresis, in situ staining of DNA fragmentation, and cell-free assays of relevant enzymatic activities...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29331939/volumetric-muscle-loss-injury-repair-using-in-situ-fibrin-gel-cast-seeded-with-muscle-derived-stem-cells-mdscs
#2
Nadine Matthias, Samuel D Hunt, Jianbo Wu, Jonathan Lo, Laura A Smith Callahan, Yong Li, Johnny Huard, Radbod Darabi
Volumetric muscle defect, caused by trauma or combat injuries, is a major health concern leading to severe morbidity. It is characterized by partial or full thickness loss of muscle and its bio-scaffold, resulting in extensive fibrosis and scar formation. Therefore, the ideal therapeutic option is to use stem cells combined with bio-scaffolds to restore muscle. For this purpose, muscle-derived stem cells (MDSCs) are a great candidate due to their unique multi-lineage differentiation potential. In this study, we evaluated the regeneration potential of MDSCs for muscle loss repair using a novel in situ fibrin gel casting...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29331830/fetal-development-of-the-poeppig-s-woolly-monkey-lagothrix-poeppigii
#3
Rafael Dos Santos de Andrade, Frederico Ozanan Barros Monteiro, Hani Rocha El Bizri, Wilter Ricardo Russiano Vicente, Diva Anelie de Araujo Guimarães, Pedro Mayor
The intrauterine development is an evolutionary strategy that prepares the neonate for extra-uterine life, thus providing important information on the life history of species. In this study, we described the external and internal morphology of 25 fetuses of Poeppig's woolly monkeys (Lagothrix poeppigii) by taking advantage of a 10-year participatory collection of biological samples originated from animals hunted for subsistence purposes in the Peruvian Amazon. Logistic regressions estimated the probability of occurrence of each external morphological characteristic in relation to the crown-rump length (CRL)...
December 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29331783/comparative-study-of-novel-in-situ-decorated-porous-chitosan-selenium-scaffolds-and-porous-chitosan-silver-scaffolds-towards-antimicrobial-wound-dressing-application
#4
Dhee P Biswas, Neil M O'Brien-Simpson, Eric C Reynolds, Andrea J O'Connor, Phong A Tran
Dermal defects caused by trauma or disease are challenging to treat due to difficult-to-treat infections that impair wound healing. Due to the widespread emergence of drug-resistant bacteria and dwindling discoveries of new antibiotics, there is currently an urgent need to introduce novel antimicrobials effective against antibiotic-resistant bacteria without causing damage to host tissues. As selenium (Se) and silver (Ag) are known for their antimicrobial properties, we investigated the separate loading of these materials into porous chitosan/PVA (CS) scaffolds through a simple in situ deposition method to create two distinct wound dressing materials (CS-Se and CS-Ag)...
January 5, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331781/a-step-wise-self-assembly-approach-in-preparation-of-multi-responsive-poly-styrene-co-methyl-methacrylate-nanoparticles-containing-spiropyran
#5
Fahimeh Khakzad, Ali Reza Mahdavian, Hamid Salehi-Mobarakeh, Mohammad Hossain Sharifian
HYPOTHESIS: Surfactant-free emulsion polymerization has become favorable due to circumventing instability issues reasonably. Incorporation of an appropriate hydrophilic macroRAFT, could provide controlled in-situ self-assembly via copolymerization with hydrophobic monomers into polymer particles. So far, this approach has mostly been studied in dispersion systems and further studies are needed in emulsions. Beside the corresponding mechanistic studies, the prepared latex particles would potentially exhibit smart behaviors by choosing stimuli-responsive monomers...
January 4, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331662/exposure-to-natural-environments-and-photographs-of-natural-environments-promotes-more-positive-body-image
#6
Viren Swami, David Barron, Adrian Furnham
Five studies were conducted to understand the impact of nature exposure on body image. In three studies using different designs and outcome measures, British university students were exposed to photographs of natural or built environments. Results indicated that exposure to images of natural, but not built, environments resulted in improved state body image. In Study 4, British community participants went on a walk in a natural or built environment, with results indicating that the walk in a natural environment resulted in significantly higher state body appreciation, whereas the walk in a built environment resulted in significantly lower scores...
January 11, 2018: Body Image
https://www.readbyqxmd.com/read/29331660/persistent-contamination-of-heater-cooler-units-for-extracorporal-circulation-cured-by-chlorhexidine-alcohol-in-water-tanks
#7
Sara Romano-Bertrand, Marine Evrevin, Chloé Dupont, Jean-Marc Frapier, Jean-Claude Sinquet, Elodie Bousquet, Bernard Albat, Estelle Jumas-Bilak
Recently, surgical site infections due to non-tuberculous Mycobacterium (NTM) have been linked to heater-cooler units (HCUs) contamination. ECDC and manufacturers now recommend the use of hydrogen peroxide in filtered water to fill HCU tanks. After implementation of these measures in our hospital, HCUs became heavily contaminated by opportunistic waterborne pathogens such as Pseudomonas aeruginosa and Stenotrophomonas maltophilia. No NTM were detected but fast-growing resistant bacteria could impair their detection...
January 10, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29331284/effects-of-an-experimental-heat-wave-on-fatty-acid-composition-in-two-mediterranean-seagrass-species
#8
Pedro Beca-Carretero, Freddy Guihéneuf, Lázaro Marín-Guirao, Jaime Bernardeau-Esteller, Rocío García-Muñoz, Dagmar B Stengel, Juan M Ruiz
Global warming is emerging as one of the most critical threats to terrestrial and marine species worldwide. This study assessed the effects of simulated warming events in culture on two seagrass species, Posidonia oceanica and Cymodocea nodosa, which play a key role in coastal ecosystems of the Mediterranean Sea. Changes in fatty acids as key metabolic indicators were assessed in specimens from two geographical populations of each species adapted to different in situ temperature regimes. Total fatty acid (TFA) content and composition were compared in C...
January 10, 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29331034/effects-of-in-situ-selenium-exposure-and-maternal-transfer-on-survival-and-deformities-of-brown-trout-salmo-trutta-fry
#9
Sean M Covington, Rami B Naddy, Alan L Prouty, Steven A Werner, Mark Dunn Lewis
Offspring of wild adult brown trout exposed to a range of selenium (Se) concentrations were reared in a laboratory setting to primarily assess effects on survival and deformities. Maternal whole body Se concentrations ranged from 4.7 to 22.6 mg/kg dry weight (dw) for wild fish. Corresponding Se concentrations in embryos ranged from 6.2 to 40.3 mg/kg dw. Significant relationships were found between embryo and whole-body tissue concentrations. Increasing egg Se concentrations were correlated with decreasing survival; however, hatch success was not significantly correlated with increasing embryo Se...
January 13, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29330925/in-situ-growth-of-mno2-nanosheets-on-n-doped-carbon-nanotubes-derived-from-polypyrrole-tubes-for-supercapacitors
#10
Xu Ou, Qi Li, Dan Xu, Jiangna Guo, Feng Yan
Nitrogen-doped porous carbon nanotubes@MnO2 (N-CNTs@MnO2) nanocomposites are prepared through the in situ growth of MnO2 nanosheets on N-CNTs derived from polypyrrole nanotubes (PNTs). Benefiting from the synergistic effects between N-CNTs (high conductivity and N doping level) and MnO2 nanosheets (high theoretical capacity), the as-prepared N-CNTs@MnO2-800 nanocomposites show a specific capacitance of 219 F g-1 at a current density of 1.0 A g-1, which is higher than that of pure MnO2 nanosheets (128 F g-1) and PNTs (42 F g-1) in 0...
January 13, 2018: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/29330909/catalytic-and-atom-economic-csp3-csp3-bond-formation-%C3%AE-to-nitrogen-alkyl-tantalum-ureates-for-hydroaminoalkylation
#11
Rebecca C DiPucchio, Sorin-Claudiu Rosca, Laurel Schafer
Atom-economic and regioselective Csp3-Csp3 bond formation has been achieved by rapid C-H alkylation of unprotected secondary arylamines with unactivated alkenes. The combination of Ta(CH2SiMe3)3Cl2, and a ureate N,O-chelating ligand salt gives an in situ prepared catalytic system that can realize high yields of β-alkylated aniline derivatives using either terminal or internal alkene substrates. These new catalyst systems realize C-H alkylation in as little as one hour and for the first time a 1:1 stoichiometry of alkene and amine substrates results in high yielding syntheses of isolated amine products by simple filtration and concentration...
January 13, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29330884/synapsin-iii-is-a-key-component-of-%C3%AE-synuclein-fibrils-in-lewy-bodies-of-pd-brains
#12
Francesca Longhena, Gaia Faustini, Tatiana Varanita, Michela Zaltieri, Vanessa Porrini, Isabella Tessari, Pietro Luigi Poliani, Cristina Missale, Barbara Borroni, Alessandro Padovani, Luigi Bubacco, Marina Pizzi, PierFranco Spano, Arianna Bellucci
Lewy bodies (LB) and Lewy neurites (LN), which are primarily composed of α-synuclein (α-syn), are neuropathological hallmarks of Parkinson's disease (PD) and dementia with Lewy bodies (DLB). We recently found that the neuronal phosphoprotein synapsin III (syn III) controls dopamine release via cooperation with α-syn and modulates α-syn aggregation. Here, we observed that LB and LN, in the substantia nigra of PD patients and hippocampus of one subject with DLB, displayed a marked immunopositivity for syn III...
January 13, 2018: Brain Pathology
https://www.readbyqxmd.com/read/29330752/evaluation-of-the-iron-regulatory-protein-1-interactome
#13
Gregory J Connell, Jando S Danial, Christian X Haastruthers
The interactions of iron regulatory proteins (IRPs) with mRNAs containing an iron-responsive element (IRE) is a major means through which intracellular iron homeostasis is maintained and integrated with cellular function. Although IRE-IRP interactions have been proposed to modulate the expression of a diverse number of mRNAs, a transcriptome analysis of the interactions that form within the native mRNA structure and cellular environment has not previously been described. An RNA-CLIP study is described here that identified IRP-1 interactions occurring within a primary cell line expressing physiologically relevant amounts of mRNA and protein...
January 12, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29330750/immunologic-and-gene-expression-profiles-of-spontaneous-canine-oligodendrogliomas
#14
Anna Filley, Mario Henriquez, Tanmoy Bhowmik, Brij Nath Tewari, Xi Rao, Jun Wan, Margaret A Miller, Yunlong Liu, R Timothy Bentley, Mahua Dey
Malignant glioma (MG), the most common primary brain tumor in adults, is extremely aggressive and uniformly fatal. Several treatment strategies have shown significant preclinical promise in murine models of glioma; however, none have produced meaningful clinical responses in human patients. We hypothesize that introduction of an additional preclinical animal model better approximating the complexity of human MG, particularly in interactions with host immune responses, will bridge the existing gap between these two stages of testing...
January 12, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29330722/location-of-low-copy-genes-in-chromosomes-of-brachiaria-spp
#15
Thaís Furtado Nani, James C Schnable, Jacob D Washburn, Patrice Albert, Welison Andrade Pereira, Fausto Souza Sobrinho, James A Birchler, Vânia Helena Techio
Repetitive DNA sequences have been widely used in cytogenetic analyses. The use of gene sequences with a low-copy-number, however, is little explored especially in plants. To date, the karyotype details in Brachiaria spp. are limited to the location of rDNA sites. The challenge lies in developing new probes based on incomplete sequencing data for the genus or complete sequencing of related species, since there are no model species with a sequenced genome in Brachiaria spp. The present study aimed at the physical location of conserved genes in chromosomes of Brachiaria ruziziensis, Brachiaria brizantha, and Brachiaria decumbens using RNAseq data, as well as sequences of Setaria italica and Sorghum bicolor through the fluorescent in situ hybridization technique...
January 12, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29330535/the-stoichiometry-of-coral-dinoflagellate-symbiosis-carbon-and-nitrogen-cycles-are-balanced-in-the-recycling-and-double-translocation-system
#16
Yasuaki Tanaka, Atsushi Suzuki, Kazuhiko Sakai
Symbioses between microalgae and animal hosts have the advantage of acquiring and sharing autotrophically produced organic carbon (C) as their energy source. However, the stoichiometry and turnover rates of biological elements in symbioses are not fully understood because of complicated metabolic interactions. We report the first comprehensive and simultaneous measurement of C and nitrogen (N) flows through coral-dinoflagellate symbiosis by using the unique approach of dual-isotope labeling with 13C and 15N, in situ chasing, and isotope-mixing models...
January 12, 2018: ISME Journal
https://www.readbyqxmd.com/read/29330532/methanobactin-from-methylosinus-trichosporium-ob3b-inhibits-n2o-reduction-in-denitrifiers
#17
Jin Chang, Wenyu Gu, Doyoung Park, Jeremy D Semrau, Alan A DiSpirito, Sukhwan Yoon
Methanotrophs synthesize methanobactin, a secondary metabolite that binds copper with an unprecedentedly high affinity. Such a strategy may provide methanotrophs a "copper monopoly" that can inhibit the activity of copper-containing enzymes of other microbes, e.g., copper-dependent N2O reductases. Here, we show that methanobactin from Methylosinus trichosporium OB3b inhibited N2O reduction in denitrifiers. When Pseudomonas stutzeri DCP-Ps1 was incubated in cocultures with M. trichosporium OB3b or with purified methanobactin from M...
January 12, 2018: ISME Journal
https://www.readbyqxmd.com/read/29330459/a-novel-ultra-stable-monomeric-green-fluorescent-protein-for-direct-volumetric-imaging-of-whole-organs-using-clarity
#18
Daniel J Scott, Natalie J Gunn, Kelvin J Yong, Verena C Wimmer, Nicholas A Veldhuis, Leesa M Challis, Mouna Haidar, Steven Petrou, Ross A D Bathgate, Michael D W Griffin
Recent advances in thick tissue clearing are enabling high resolution, volumetric fluorescence imaging of complex cellular networks. Fluorescent proteins (FPs) such as GFP, however, can be inactivated by the denaturing chemicals used to remove lipids in some tissue clearing methods. Here, we solved the crystal structure of a recently engineered ultra-stable GFP (usGFP) and propose that the two stabilising mutations, Q69L and N164Y, act to improve hydrophobic packing in the core of the protein and facilitate hydrogen bonding networks at the surface, respectively...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330455/towards-electrotuneable-nanoplasmonic-fabry-perot-interferometer
#19
Hayley Weir, Joshua B Edel, Alexei A Kornyshev, Debabrata Sikdar
Directed voltage-controlled assembly and disassembly of plasmonic nanoparticles (NPs) at electrified solid-electrolyte interfaces (SEI) offer novel opportunities for the creation of tuneable optical devices. We apply this concept to propose a fast electrotuneable, NP-based Fabry-Perot (FP) interferometer, comprising two parallel transparent electrodes in aqueous electrolyte, which form the polarizable SEI for directed assembly-disassembly of negatively charged NPs. An FP cavity between two reflective NP-monolayers assembled at such interfaces can be formed or deconstructed under positive or negative polarization of the electrodes, respectively...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330430/carbon-doped-sns2-nanostructure-as-a-high-efficiency-solar-fuel-catalyst-under-visible-light
#20
Indrajit Shown, Satyanarayana Samireddi, Yu-Chung Chang, Raghunath Putikam, Po-Han Chang, Amr Sabbah, Fang-Yu Fu, Wei-Fu Chen, Chih-I Wu, Tsyr-Yan Yu, Po-Wen Chung, M C Lin, Li-Chyong Chen, Kuei-Hsien Chen
Photocatalytic formation of hydrocarbons using solar energy via artificial photosynthesis is a highly desirable renewable-energy source for replacing conventional fossil fuels. Using an L-cysteine-based hydrothermal process, here we synthesize a carbon-doped SnS2 (SnS2-C) metal dichalcogenide nanostructure, which exhibits a highly active and selective photocatalytic conversion of CO2 to hydrocarbons under visible-light. The interstitial carbon doping induced microstrain in the SnS2 lattice, resulting in different photophysical properties as compared with undoped SnS2...
January 12, 2018: Nature Communications
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