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https://www.readbyqxmd.com/read/28817827/role-of-next-generation-sequencing-as-a-diagnostic-tool-for-the-evaluation-of-bone-and-soft-tissue-tumors
#1
Kinga Szurian, Karl Kashofer, Bernadette Liegl-Atzwanger
Bone and soft-tissue tumors are in general rare. Diagnosing these tumors is challenging based on the significant number of different tumor entities, the rareness of these tumors, and the considerable morphological heterogeneity which can be found within a single tumor entity. Considering that more than half of the described soft-tissue tumors and approximately 25% of the bone tumors harbor recurrent genetic alterations, the use of auxiliary molecular examinations should be strongly considered. Molecular analyses are important to confirm the diagnosis, to guide treatment, to provide information about prognosis, and to allow patient recruitment for basket trials based on the molecular signature of a tumor...
August 18, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28817802/molecular-memory-of-morphologies-by-septins-during-neuron-generation-allows-early-polarity-inheritance
#2
Leila Boubakar, Julien Falk, Hugo Ducuing, Karine Thoinet, Florie Reynaud, Edmund Derrington, Valérie Castellani
Transmission of polarity established early during cell lineage history is emerging as a key process guiding cell differentiation. Highly polarized neurons provide a fascinating model to study inheritance of polarity over cell generations and across morphological transitions. Neural crest cells (NCCs) migrate to the dorsal root ganglia to generate neurons directly or after cell divisions in situ. Using live imaging of vertebrate embryo slices, we found that bipolar NCC progenitors lose their polarity, retracting their processes to round for division, but generate neurons with bipolar morphology by emitting processes from the same locations as the progenitor...
August 16, 2017: Neuron
https://www.readbyqxmd.com/read/28817651/comparative-genetic-analysis-of-the-45s-rdna-intergenic-spacers-from-three-saccharum-species
#3
Yongji Huang, Fan Yu, Xueting Li, Ling Luo, Jiayun Wu, Yongqing Yang, Zuhu Deng, Rukai Chen, Muqing Zhang
The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For the first time, we sequenced and analyzed the entire IGS region from three original species within the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum in this study. We have compared the IGS organization within three original species of the genus Saccharum. The IGS of these three original species showed similar overall organizations comprised of putative functional elements needed for rRNA gene activity as well as a non-transcribed spacer (NTS), a promoter region, and an external transcribed spacer (ETS)...
2017: PloS One
https://www.readbyqxmd.com/read/28817612/expression-and-functional-implications-of-the-renal-apelinergic-system-in-rodents
#4
Anne-Marie O'Carroll, Sabrine Salih, Philip R Griffiths, Aarifah Bijabhai, Mark A Knepper, Stephen J Lolait
Apelin binds to the G protein-coupled apelin receptor (APJ; gene name aplnr) to modulate diverse physiological systems including cardiovascular function, and hydromineral and metabolic balance. Recently a second endogenous ligand for APJ, named apela, has been discovered. We confirm that apela activates signal transduction pathways (ERK activation) in cells expressing the cloned rat APJ. Previous studies suggest that exogenous apela is diuretic, attributable wholly or in part to an action on renal APJ. Thus far the cellular distribution of apela in the kidney has not been reported...
2017: PloS One
https://www.readbyqxmd.com/read/28817437/surgicopathological-quality-control-and-protocol-adherence-to-lymphadenectomy-in-the-critics-gastric-cancer-trial
#5
Yvette H M Claassen, Wobbe O de Steur, Henk H Hartgrink, Johan L Dikken, Johanna W van Sandick, Nicole C T van Grieken, Annemiek Cats, Anouk K Trip, Edwin P M Jansen, Willemina M Meershoek-Klein Kranenbarg, Jeffrey P B M Braak, Hein Putter, Mark I van Berge Henegouwen, Marcel Verheij, Cornelis J H van de Velde
OBJECTIVE: The purpose of this study was to evaluate surgicopathological quality and protocol adherence for lymphadenectomy in the CRITICS trial. SUMMARY OF BACKGROUND DATA: Surgical quality assurance is a key element in multimodal studies for gastric cancer. In the multicenter CRITICS trial (ChemoRadiotherapy after Induction chemotherapy In Cancer of the Stomach), patients with resectable gastric cancer were randomized for preoperative chemotherapy, followed by gastrectomy with a D1+ lymphadenectomy (removal of stations 1 to 9 and 11), followed by either chemotherapy or chemoradiotherapy...
August 16, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28817384/engineering-the-mechanical-and-biological-properties-of-nanofibrous-vascular-grafts-for-in-situ-vascular-tissue-engineering
#6
Jeffrey J D Henry, Jian Yu, Aijun Wang, Randall Lee, Jun Fang, Song Li
Synthetic small diameter vascular grafts have a high failure rate, and endothelialization is critical for preventing thrombosis and graft occlusion. A promising approach is in situ tissue engineering, whereby an acellular scaffold is implanted and provides stimulatory cues to guide the in situ remodeling into a functional blood vessel. An ideal scaffold should have sufficient binding sites for biomolecule immobilization and a mechanical property similar to native tissue. Here we developed a novel method to blend low molecular weight (LMW) elastic polymer during electrospinning process to increase conjugation sites and to improve the mechanical property of vascular grafts...
August 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/28817265/highly-efficient-performance-and-conversion-pathway-of-photocatalytic-no-oxidation-on-sro-clusters-amorphous-carbon-nitride
#7
Wen Cui, Jieyuan Li, Fan Dong, Yanjuan Sun, Guangming Jiang, Wanglai Cen, Shun Cheng Lee, Zhongbiao Wu
This work demonstrates the first molecular-level conversion pathway of NO oxidation over a novel SrO-clusters@amorphous carbon nitride (SCO-ACN) photocatalyst, which is synthesized via co-pyrolysis of urea and SrCO3. The inclusion of SrCO3 is crucial in the formation of the amorphous carbon nitride (ACN) and SrO clusters by attacking the intralayer hydrogen bonds at the edge sites of graphitic carbon nitride (CN). The amorphous nature of ACN can promote the transportation, migration, and transformation of charge carriers on SCO-ACN...
August 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28817264/structural-diversities-in-heterometallic-mn-ca-cluster-chemistry-from-the-use-of-salicylhydroxamic-acid-mn-iii-4ca2-mn-ii-iii-6ca2-mn-iii-iv-8ca-and-mn-iii-8ca2-complexes-with-relevance-to-both-high-and-low-valent-states-of-the-oxygen-evolving-complex
#8
Alysha A Alaimo, Evangelia S Koumousi, Luís Cunha-Silva, Laura J McCormick, Simon J Teat, Vassilis Psycharis, Catherine P Raptopoulou, Shreya Mukherjee, Chaoran Li, Sayak Das Gupta, Albert Escuer, George Christou, Theocharis C Stamatatos
One-pot reactions between the [Mn3O(O2CPh)6(py)x](+/0) triangular precursors and either CaBr2·xH2O or CaCl2·6H2O, in the presence of salicylhydroxamic acid (shaH2), have afforded the heterometallic complexes [Mn(III)4Ca2(O2CPh)4(shi)4(H2O)3(Me2CO)] (1) and (pyH)[Mn(II)2Mn(III)4Ca2Cl2(O2CPh)7(shi)4(py)4] (2), respectively, in good yields. Further reactions but using a more flexible synthetic scheme comprising the Mn(NO3)2·4H2O/Ca(NO3)2·4H2O and Mn(O2CPh)2·2H2O/Ca(ClO4)2·4H2O "metal blends" and shaH2, in the presence of external base NEt3, led to the new complexes (NHEt3)2[Mn(III)4Mn(IV)4Ca(OEt)2(shi)10(EtOH)2] (3) and (NHEt3)4[Mn(III)8Ca2(CO3)4(shi)8] (4), respectively...
August 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28817251/dual-core-shell-structured-s-c-mno2-nanocomposite-for-highly-stable-lithium-sulfur-batteries
#9
Lubin Ni, Gangjin Zhao, Guang Yang, Guosheng Niu, Ming Chen, Guowang Diao
Lithium-sulfur (Li-S) batteries have currently excited worldwide academic and industrial interest as a next-generation high-power energy storage system (EES) because of their high energy density and low cost of sulfur. However, the commercialization application is being hindered by capacity decay, mainly attributed to the polysulfide shuttle and poor conductivity of sulfur. Here, we have designed a novel dual core-shell nanostructure of S@C@MnO2 nanosphere hybrid as the sulfur host. The S@C@MnO2 nanosphere is successfully prepared using mesoporous hollow carbon spheres (MHCS) as the template and then in situ MnO2 growth on the surface of MHCS...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28817123/evaluation-of-telomere-length-in-human-cardiac-tissues-using-cardiac-quantitative-fish
#10
Maryam Sharifi-Sanjani, Alan K Meeker, Foteini Mourkioti
Telomere length has been correlated with various diseases, including cardiovascular disease and cancer. The use of currently available telomere-length measurement techniques is often restricted by the requirement of a large amount of cells (Southern-based techniques) or the lack of information on individual cells or telomeres (PCR-based methods). Although several methods have been used to measure telomere length in tissues as a whole, the assessment of cell-type-specific telomere length provides valuable information on individual cell types...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28817100/modulatory-effects-of-exogenously-applied-polyamines-on-postharvest-physiology-antioxidant-system-and-shelf-life-of-fruits-a-review
#11
REVIEW
Sunil Sharma, Sunil Pareek, Narashans Alok Sagar, Daniel Valero, Maria Serrano
Polyamines (PAs) are natural compounds involved in many growth and developmental processes in plants, and, specifically in fruits, play a vital role regulating its development, ripening and senescence processes. Putrescine (PUT), spermine (SPE), and spermidine (SPD) are prominent PAs applied exogenously to extend shelf life of fruits. They also originate endogenously during developmental phases of horticultural crops and simultaneously affect the quality attributes and shelf life. Their anti-ethylene nature is being exploited to enhance the shelf life when exogenously applied on fruits...
August 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28817081/multiphysics-simulation-of-low-amplitude-acoustic-wave-detection-by-piezoelectric-wafer-active-sensors-validated-by-in-situ-ae-fatigue-experiment
#12
Md Yeasin Bhuiyan, Victor Giurgiutiu
Piezoelectric wafer active sensors (PWAS) are commonly used for detecting Lamb waves for structural health monitoring application. However, in most applications of active sensing, the signals are of high-amplitude and easy to detect. In this article, we have shown a new avenue of using the PWAS transducer for detecting the low-amplitude fatigue-crack related acoustic emission (AE) signals. Multiphysics finite element (FE) simulations were performed with two PWAS transducers bonded to the structure. Various configurations of the sensors were studied by using the simulations...
August 17, 2017: Materials
https://www.readbyqxmd.com/read/28816741/reflectance-confocal-microscopy-criteria-of-pigmented-squamous-cell-carcinoma-in-situ
#13
Neda Shahriari, Jane M Grant-Kels, Harold S Rabinovitz, Margaret Oliviero, Alon Scope
Pigmented squamous cell carcinoma in situ (pSCCis) is difficult to diagnose based on clinical and dermoscopic examination. Reflectance confocal microscopy (RCM) allows noninvasive differentiation between malignant and benign pigmented skin lesions. We determined the frequency of key RCM features of pSCCis and correlated the RCM criteria with the corresponding dermoscopic and histopathologic criteria. The study included 28 lesions with biopsy-proven diagnosis of pSCCis derived from 28 patients. Clinical, dermoscopic, and RCM images of these lesions were retrospectively analyzed by 3 independent observers...
August 9, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28816603/analysis-of-the-small-non-protein-coding-rna-profile-of-mouse-spermatozoa-reveals-specific-enrichment-of-pirnas-within-mature-spermatozoa
#14
Kate Hutcheon, Eileen A McLaughlin, Simone J Stanger, Ilana R Bernstein, Matthew D Dun, Andrew L Eamens, Brett Nixon
Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition of which is markedly influenced by their epididymal transit. Thus, although miRNAs are highly represented in the spermatozoa of the proximal epididymis, this sRNA class is largely diminished in mature spermatozoa of the distal epididymis. Coincident with this, a substantial enrichment in Piwi-interacting RNA (piRNA) abundance in cauda spermatozoa was detected...
August 17, 2017: RNA Biology
https://www.readbyqxmd.com/read/28816458/pressure-induced-emission-enhancement-of-carbazole-the-restriction-of-intramolecular-vibration
#15
Yarong Gu, Kai Wang, Yu-Xiang Dai, Guanjun Xiao, Yuguo Ma, Yuancun Qiao, Bo Zou
Pressure-induced emission enhancement (PIEE), a novel phenomenon on the enhancement of the solid-state emission efficiency of fluorophores, has been arousing wide attention in recent years. However, researches on PIEE are still in the early stage. To further pursue more enhanced efficiency, discovering and designing more PIEE systems would be urgently desirable and of great importance. In this letter, we found carbazole presented a conspicuous emission enhancement under high pressure up to 1.0 GPa. In situ high-pressure infrared spectroscopy, angle-dispersive X-ray diffraction analysis combined with Hirshfeld surface theory calculation indicated that the PIEE of carbazole was attributed to the decrease of non-radiation vibration process...
August 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28816445/tracking-the-progress-and-mechanism-study-of-a-solvothermal-in-situ-domino-n-alkylation-reaction-of-triethylamine-and-ammonia-assisted-by-ferrous-sulfate
#16
Jin-Ping Zhong, Bin Liu, Tao Yang, Yue-Jin Liu, Zhong-Hong Zhu, Bing-Feng Shi, Mohamedally Kurmoo, Ming-Hua Zeng
Atom economic in situ domino N-alkylation reactions of triethylamine/ammonia with 2-(hydroxymethyl)quinolin-8-ol (HL-OH) assisted by FeSO4·7H2O were realized under mild solvothermal conditions at 120 °C in acetonitrile. The resulting tripodal 2,2',2″-[nitrilotris(methylene)]tris(quinolin-8-ol) (H3L3-N) forms a linear trimer [Fe3(L3-N)2] (1). Electrospray ionization mass spectrometry of the reaction solution provides evidence for the intermediates of three steps, while crystallography and X-ray photoelectron spectroscopy characterize the trimer...
August 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28816432/an-integrated-microsystem-for-real-time-detection-and-threshold-activated-treatment-of-bacterial-biofilms
#17
Sowmya Subramanian, Ekaterina I Tolstaya, Thomas Winkler, William E Bentley, Reza Ghodssi
Bacterial biofilms are the primary cause of infections in medical implants and catheters. Delayed detection of biofilm infections contributes to the widespread use of high doses of antibiotics, leading to the emergence of antibiotic-resistant bacterial strains. Accordingly, there is an urgent need for systems that can rapidly detect and treat biofilm infections in situ. As a step towards this goal, in this work we have developed for the first time a threshold-activated feedback-based impedance sensor-treatment system for combined real-time detection and treatment of biofilms...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28816304/iodoarene-catalyzed-oxidative-transformations-using-molecular-oxygen
#18
K Miyamoto, J Yamashita, S Narita, Y Sakai, K Hirano, T Saito, C Wang, M Ochiai, M Uchiyama
Molecular oxygen serves as a useful oxidant for the glycol scission of 1,2-diols and the Hofmann rearrangement of primary amides using pentamethyliodobenzene as a catalyst. The use of isobutyraldehyde and Lewis basic nitriles under O2 enabled the iodine(i)/(iii) catalytic cycle, where in situ-generated peracid acts as a terminal oxidant.
August 17, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28816270/-clinical-value-of-fluorescence-in-situ-hybridization-positive-of-exfoliated-urothelial-cells-in-urothelial-carcinoma
#19
J Y Liu, X Peng, X H Ning, T Li, S H Peng, J Y Wang, S J Liu, Y Ding, L Cai, K Gong
OBJECTIVE: To analyze the clinical pathologic characteristics of cases with fluorescence in situ hybridization (FISH) positive of exfoliated urothelial cells, so as to evaluate the clinical utility of FISH in the diagnosis of urothelial carcinoma (UC). METHODS: A total of 271 cases of FISH positive in Department of Urology of Peking University First Hospital from Apr. 2012 to Sep. 2015 were recruited in this study. Retrospective analysis was made on their clinical data...
August 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28815945/development-of-a-quantitative-method-for-the-analysis-of-cocaine-analogue-impregnated-into-textiles-by-raman-spectroscopy
#20
Linda Xiao, Rhiannon Alder, Megha Mehta, Nadine Krayem, Bianca Cavasinni, Sean Laracy, Shane Cameron, Shanlin Fu
Cocaine trafficking in the form of textile impregnation is routinely encountered as a concealment method. Raman spectroscopy has been a popular and successful testing method used for in situ screening of cocaine in textiles and other matrices. Quantitative analysis of cocaine in these matrices using Raman spectroscopy has not been reported to date. This study aimed to develop a simple Raman method for quantifying cocaine using atropine as the model analogue in various types of textiles. Textiles were impregnated with solutions of atropine in methanol...
August 16, 2017: Drug Testing and Analysis
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