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https://www.readbyqxmd.com/read/28655165/investigation-of-natural-radioactivity-as-a-possible-driver-of-cancer-incidence-in-para%C3%A3-ba-northeastern-brazil
#1
Josineide Marques do Nascimento Santos, José Araújo Dos Santos Júnior, Ana Maria Mendoça de Albuquerque Melo, Romilton Dos Santos Amaral, Rômulo Simões Cezar Menezes, Zahily Herrero Fernández, Lino Angel Valcárcel Rojas, Jairo Dias Bezerra, Alberto Antônio da Silva, Kennedy Francys Rodrigues Damascena, Nilson Vicente da Silva Medeiros
An increase of health problems has been reported in the city of Triunfo, Paraíba, NE Brazil. This pattern has been attributed to environmental radiation since there are uranium deposits in the neighboring municipalities. The present study aimed to carry a radiometric survey in Triunfo to determine effective dose from external outdoor radiation exposure. The results were obtained through 'in situ' measurements using a gamma spectrometry system. The results ranged from 0.06 to 0.38 mSv/y, mean of 0.20 mSv/y...
June 24, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/28655112/real-time-monitoring-of-the-degradation-of-cu-ii-edta-in-h2o2-uv-using-illumination-assisted-droplet-spray-ionization-mass-spectrometry
#2
Hong Zhang, Na Li, Yingying Wang, Dandan Zhao, Jing He, Hong You, Jie Jiang
Illumination-assisted droplet spray ionization (IA-DSI) mass spectrometry was developed for in situ analysis and real-time monitoring of photolysis reactions. In IA-DSI, a photolysis reaction occurs on a corner of a cover slip positioned in front of a mass spectrometer, and the generated reaction intermediates are ionized via the corner and are directed to the inlet and analyzed by a mass spectrometer. IA-DSI is contaminant-free, low-cost and simple, and was demonstrated for in situ analysis and real-time monitoring of the degradation of Cu(II)-EDTA in H2O2/UV...
June 19, 2017: Chemosphere
https://www.readbyqxmd.com/read/28654985/transcriptomic-profiling-of-posterior-polymorphous-corneal-dystrophy
#3
Doug D Chung, Ricardo F Frausto, Benjamin R Lin, Evelyn M Hanser, Zack Cohen, Anthony J Aldave
Purpose: To investigate the molecular basis of posterior polymorphous corneal dystrophy (PPCD) by examining the PPCD transcriptome and the effect of decreased ZEB1 expression on corneal endothelial cell (CEnC) gene expression. Methods: Next-generation RNA sequencing (RNA-seq) analyses of corneal endothelium from two PPCD-affected individuals (one with PPCD3 and one of unknown genetic cause) compared with two age-matched controls, and primary human CEnC (pHCEnC) transfected with siRNA-mediated ZEB1 knockdown...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28654968/association-of-electrochemical-therapy-with-optical-mechanical-and-acoustic-impedance-properties-of-porcine-skin
#4
Wesley J Moy, Erica Su, Jason J Chen, Connie Oh, Joe C Jing, Yueqiao Qu, Youmin He, Zhongping Chen, Brian J F Wong
Importance: The classic management of burn scars and other injuries to the skin has largely relied on soft-tissue transfer to resurface damaged tissue with local tissue transfer or skin graft placement. In situ generation of electrochemical reactions using needle electrodes and an application of current may be a new approach to treat scars and skin. Objective: To examine the changes in optical, mechanical, and acoustic impedance properties in porcine skin after electrochemical therapy...
June 22, 2017: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/28654889/methylomics-of-nitroxidative-stress-on-precancerous-cells-reveals-dna-methylation-alteration-at-the-transition-from-in-situ-to-invasive-cervical-cancer
#5
Po-Hsuan Su, Yao-Wen Hsu, Rui-Lan Huang, Yu-Chun Weng, Hui-Chen Wang, Yu-Chih Chen, Yueh-Ju Tsai, Chiou-Chung Yuan, Hung-Cheng Lai
Epigenetic dysregulation is important in cervical cancer development, but the underlying mechanism is largely unknown. Increasing evidence indicates that DNA methylation is sensitive to changes in microenvironmental factors, such as nitric oxide (NO) in the chronic inflammatory cervix. However, the epigenomic effects of NO in cancer have not been investigated. In this study, we explored the methylomic effects of nitroxidative stress in HPV-immortalized precancerous cells. Chronic NO exposure promoted the acquisition of malignant phenotypes such as cell growth, migration, invasion, and anchorage-independent growth...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28654887/glucose-oxidase-initiated-cascade-catalysis-for-sensitive-impedimetric-aptasensor-based-on-metal-organic-frameworks-functionalized-with-pt-nanoparticles-and-hemin-g-quadruplex-as-mimicking-peroxidases
#6
Xingxing Zhou, Shijing Guo, Jiaxi Gao, Jianmin Zhao, Shuyan Xue, Wenju Xu
Based on cascade catalysis amplification driven by glucose oxidase (GOx), a sensitive electrochemical impedimetric aptasensor for protein (carcinoembryonic antigen, CEA as tested model) was proposed by using Cu-based metal-organic frameworks functionalized with Pt nanoparticles, aptamer, hemin and GOx (Pt@CuMOFs-hGq-GOx). CEA aptamer loaded onto Pt@CuMOFs was bound with hemin to form hemin@G-quadruplex (hGq) with mimicking peroxidase activity. Through sandwich-type reaction of target CEA and CEA aptamers (Apt1 and Apt2), the obtained Pt@CuMOFs-hGq-GOx as signal transduction probes (STPs) was captured to the modified electrode interface...
June 20, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28654714/reactions-of-donor-acceptor-cyclopropanes-with-naphthoquinones-by-an-in-situ-umpolung
#7
Daniel B Werz, Alexander Lücht, Lukas J Patalag, Peter G Jones, André U Augustin
Reactions of 2-aryl cyclopropane dicarboxylates with naphthoquinones are reported. The key feature was the use of catalytic amounts of SnCl2, which acts as both electron donor and Lewis acid. By an in-situ umpolung of naphthoquinone the formerly electrophilic species is converted into a nucleophile that is able to trigger the ring-opening of the three-membered ring with formation of a new C-C bond. Treatment of these products with base under oxidative conditions resulted - by losing methyl formate - in cyclopentannulated products with fully conjugated π systems exhibiting intensive absorptions in the visible range...
June 27, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28654634/emergence-of-met-hyper-amplification-at-progression-to-met-and-braf-inhibition-in-colorectal-cancer
#8
Daniele Oddo, Giulia Siravegna, Annunziata Gloghini, Claudio Vernieri, Benedetta Mussolin, Federica Morano, Giovanni Crisafulli, Rosa Berenato, Giorgio Corti, Chiara Costanza Volpi, Michela Buscarino, Monica Niger, Philip D Dunne, Giuseppe Rospo, Emanuele Valtorta, Alice Bartolini, Giovanni Fucà, Simona Lamba, Antonia Martinetti, Maria Di Bartolomeo, Filippo de Braud, Alberto Bardelli, Filippo Pietrantonio, Federica Di Nicolantonio
BACKGROUND: Combined MET and BRAF inhibition showed clinical benefit in a patient with rectal cancer carrying BRAF(V600E) and MET amplification. However after 4 months, acquired resistance emerged and the patient deceased shortly after disease progression. The mechanism of resistance to this drug combination is unknown. METHODS: We analysed plasma circulating tumour DNA obtained at progression by exome sequencing and digital PCR. MET gene and mRNA in situ hybridisation analyses in two bioptic specimens obtained at progression were used to confirm the plasma data...
June 27, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28654596/a-microbiological-and-ultrastructural-comparison-of-aseptic-versus-sterile-acellular-dermal-matrix-as-a-reconstructive-material-and-a-scaffold-for-stem-cell-ingrowth
#9
Shaun D Mendenhall, Ryan W Schmucker, Timothy H F Daugherty, Katherine M Kottwitz, Joel D Reichensperger, Janak Koirala, Paul S Cederna, Michael W Neumeister
BACKGROUND: Recent data suggest an increased risk for infection when acellular dermal matrix is used in breast reconstruction. This may be because some acellular dermal matrices are actually not terminally sterilized but are instead "aseptically processed." This study evaluates aseptic and sterile matrices for evidence of bacterial contamination and whether or not terminal sterilization affects matrix collagen architecture and stem cell ingrowth. METHODS: Five separate samples of 14 different matrices were analyzed by fluorescent in situ hybridization using a bacterial DNA probe to detect bacterial DNA on the matrices...
July 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28654428/residual-pure-intralymphatic-breast-carcinoma-following-neoadjuvant-chemotherapy-is-indicative-of-poor-clinical-outcome-even-in-node-negative-patients
#10
Esther Cheng, David Ko, Michaela Nguyen, Tracy-Ann Moo, Eleni Andreopoulou, Syed A Hoda, Timothy M D'Alfonso
Residual carcinoma confined to lymphovascular spaces following neoadjuvant chemotherapy (NAC) for invasive breast carcinoma is an uncommon finding. We studied pathologic features and outcome for patients with pure intralymphatic carcinoma (PIC) following NAC, a pattern of residual disease reported to have a poor outcome in the only previously published series of this entity. Six of 284 (2.1%) patients treated with NAC were studied. All 6 patients had axillary lymph node involvement before NAC. Tumors were triple-negative (n=3) and HER2+ (n=3: 2 ER+, 1 ER-)...
June 26, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28654369/gymnotus-coatesi-gymnotiformes-a-case-of-colocation-of-multiple-sites-of-18s-rdna-with-telomeric-sequences
#11
Milla de Andrade Machado, Adauto Lima Cardoso, Susana Suely Rodrigues Milhomem-Paixão, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Gymnotus coatesi is a small and rare species of banded knife fish that was originally described by LaMonte in 1935, found along the main stretch of the Amazon River. There is no described cytogenetic data on this species. We analyzed the karyotype of five specimens of G. coatesi collected from Cururutuia Stream in Bragança, Pará, Brazil. The obtained diploid number is 50 and the karyotypic formula is 24 m/sm +26 st/a. The constitutive heterochromatin is DAPI positive and distributed mainly in the centromeric and pericentromeric regions of the chromosomes...
June 27, 2017: Zebrafish
https://www.readbyqxmd.com/read/28654351/zz-z0-the-new-system-of-sex-chromosomes-in-eigenmannia-aff-trilineata-teleostei-gymnotiformes-sternopygidae-characterized-by-molecular-cytogenetics-and-dna-barcoding
#12
Cristian Araya-Jaime, Nadayca T Bonani Mateussi, Ricardo Utsunomia, Guilherme J Costa-Silva, Claudio Oliveira, Fausto Foresti
The cytogenetic characteristics of Eigenmannia aff. trilineata were analyzed by basic and molecular cytogenetics, applying fluorescent in situ hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11...
June 27, 2017: Zebrafish
https://www.readbyqxmd.com/read/28654298/direct-neutron-spectroscopy-observation-of-cerium-hydride-species-on-a-cerium-oxide-catalyst
#13
Zili Wu, Yongqiang Q Cheng, Franklin Feng Tao, Luke L Daemen, Guo Shiou Foo, Luan Nguyen, Xiaoyan Zhang, Ariana Beste, Anibal J Ramirez-Cuesta
Ceria has recently shown intriguing hydrogenation reactivity in catalyzing alkyne selectively to alkenes. However, the mecha-nism of the hydrogenation reaction, especially the activation of H2, remains experimentally elusive. In this work, we report the first direct spec-troscopy evidence for the presence of both surface and bulk Ce-H species upon H2 dissociation over ceria via in situ inelastic neutron scattering (INS) spectroscopy. Combined with in situ ambient pressure X-ray photoelectron spectroscopy (AP-XPS), IR and Raman spectroscopic studies, the results together point to a heterolytic dissociation mechanism of H2 over ceria, leading to either homolytic products (surface OHs) on close-to-stoichiometric ceria surface or heterolytic products (Ce-H and OH) with the presence of induced oxygen vacancies in ceria...
June 27, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28654289/oxidation-of-natural-bioactive-flavonolignan-2-3-dehydrosilybin-an-electrochemical-and-spectral-study
#14
Romana Sokolova, Jana Kocabova, Petr Marhol, Jan Fiedler, David Biedermann, Jan Vacek, Vladimir Kren
The electrochemical oxidation of the natural antioxidant 2,3-dehydrosilybin (DHS) was investigated in acetonitrile. The spectral changes during two electron and two proton oxidation registered by in situ IR spectroelectrochemistry show that the electron transfer is followed by a subsequent chemical reaction with traces of water. A benzofuranone derivative (BF) is formed by ECEC (electron transfer - chemical reaction - electron transfer - chemical reaction) process at the potential of the first oxidation wave...
June 27, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28654270/ultralong-hydroxyapatite-nanowires-based-paper-co-loaded-with-silver-nanoparticles-and-antibiotic-for-long-term-antibacterial-benefit
#15
Zhi-Chao Xiong, Zi-Yue Yang, Ying-Jie Zhu, Fei-Fei Chen, Yong-Gang Zhang, Ri-Long Yang
Hydroxyapatite is a kind of biocompatible, environmentally friendly, and versatile inorganic biomaterial. Herein, the preparation of ultralong hydroxyapatite nanowires (HAPNWs)-based antibacterial paper co-loaded with silver nanoparticles (AgNPs) and antibiotic is reported. HAPNWs are used to prepare AgNPs in situ using an aqueous solution containing AgNO3 under the sunlight without added reducing agent at room temperature. Subsequently, ciprofloxacin (CIP) as an antibiotic is loaded on the HAPNWs@AgNPs. The resultant HAPNWs@AgNPs-CIP paper possesses several unique properties, including high flexibility, high Brunauer-Emmett-Teller (BET) specific surface area (47...
June 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28654237/light-induced-proton-pumping-with-a-semiconductor-vision-for-photoproton-lateral-separation-and-robust-manipulation
#16
Hanna M Maltanava, Sergey K Poznyak, Daria V Andreeva, Marcela C Quevedo, Alexandre C Bastos, João Tedim, Mario G S Ferreira, Ekaterina V Skorb
Energy transfer reactions are the key for living open systems, biological chemical networking and development of life inspired nanoscale machineries. It is a challenge to find simple reliable synthetic chemical networks providing a localization of the time dependent flux of matter. In this paper we look to photocatalytic reaction on TiO2 from different angle focusing on proton generation and introduce a reliable, minimal reagent consuming, stable inorganic light promoted proton pump. Localized illumination was applied to a TiO2 surface in solution for reversible spatially controlled "inorganic photoproton" isometric cycling, the lateral separation of water splitting reactions...
June 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28654127/bio-camouflage-of-anatase-nanoparticles-explored-by-in-situ-high-resolution-electron-microscopy
#17
Ana R Ribeiro, Arijita Mukherjee, Xuan Hu, Shayan Shafien, Reza Ghodsi, Kun He, Sara Gemini-Piperni, Canhui Wang, Robert F Klie, Tolou Shokuhfar, Reza Shahbazian-Yassar, Radovan Borojevic, Luis A Rocha, José M Granjeiro
While titanium is the metal of choice for most prosthetics and inner body devices due to its superior biocompatibility, the discovery of Ti-containing species in the adjacent tissue as a result of wear and corrosion has been associated with autoimmune diseases and premature implant failures. Here, we utilize the in situ liquid cell transmission electron microscopy (TEM) in a liquid flow holder and graphene liquid cells (GLCs) to investigate, for the first time, the in situ nano-bio interactions between titanium dioxide nanoparticles and biological medium...
June 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28654114/functionalized-metal-organic-frameworks-for-effective-capture-of-radioactive-organic-iodides
#18
Baiyan Li, Xinglong Dong, Hao Wang, Dingxuan Ma, Kui Tan, Zhan Shi, Yves J Chabal, Yu Han, Jing Li
Highly efficient capture of radioactive organic iodides (ROIs) from off-gas mixtures remains a substantial challenge for nuclear waste treatment. Current materials utilized for ROI sequestration suffer from low capacity, high cost (e.g. use of noble metals), and poor recyclability. Recently, we have developed a new strategy to tackle this challenge by functionalizing MOF materials with tertiary amines to create molecular traps for the effective capture and removal of ROIs (e.g. radioactive methyl iodide) from nuclear wastes...
June 27, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28654107/in-situ-cathodic-activation-of-v-incorporated-nixsy-nanowires-for-enhanced-hydrogen-evolution
#19
Xiao Shang, Kai-Li Yan, Yi Rao, Bin Dong, Jing-Qi Chi, Yan-Ru Liu, Xiao Li, Yong-Ming Chai, Chen-Guang Liu
In situ cathodic activation (ISCA) of V-incorporated NixSy nanowires supported on nickel foam (VS/NixSy/NF) can be realized in an alkaline hydrogen evolution reaction (HER) process, which provides not only clearly enhanced activity but also ultrahigh stability for HER. The ISCA process is continuous linear sweep voltammetry (LSV) on VS/NixSy/NF as a cathodic electrode with gradually enhanced HER activity. The activated VS/NixSy/NF (A-VS/NixSy/NF) demonstrates enhanced HER activity with an overpotential of 125 mV to drive 10 mA cm(-2), which is much lower than that of other samples...
June 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28654079/in-situ-immunofluorescent-staining-of-autophagy-in-muscle-stem-cells
#20
Francesco Castagnetti, Elisabetta Fiacco, Carol Imbriano, Lucia Latella
Increasing evidence points to autophagy as a crucial regulatory process to preserve tissue homeostasis. It is known that autophagy is involved in skeletal muscle development and regeneration, and the autophagic process has been described in several muscular pathologies and age-related muscle disorders. A recently described block of the autophagic process that correlates with the functional exhaustion of satellite cells during muscle repair supports the notion that active autophagy is coupled with productive muscle regeneration...
June 12, 2017: Journal of Visualized Experiments: JoVE
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