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uterus endometrial stem cell

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https://www.readbyqxmd.com/read/29701160/non-pregnant-human-uterus-as-source-of-mesenchymal-stem-cells
#1
Noemi Eiro, Maria Fraile, Jose Schneider, Francisco Vizoso
Stem/progenitor cells for cell therapy and regenerative medicine should ideally be available in large numbers, after having been isolated using minimally invasive or non-invasive methodologies. Also, they should exhibit wide differentiation potential into multiple lineages, as well as capability to be used successfully in autologous or allogeneic transplantation, and all this in accordance with the applicable guidelines of good manufacturing practice. Thus, the identification and characterization of alternative sources of mesenchymal stem cells (MSCs) is of great importance...
April 26, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29697394/specificities-of-endometrial-proliferation-stem-cell-index-distribution-in-endometrioid-carcinoma-of-different-grade-of-malignancy
#2
N Kikalishvili, R Beriashvili, T Muzashvili, G Burkadze
Endometrial neoplasia is the most common malignant tumor of female genital system in developed countries. The incidence of endometrial cancer has increased in the last years and despite advances in diagnosis and treatment, the death rates have steadily been increasing over the past 20 years. Therefore aspects of endometrial cancer development, pathogenesis and effective treatment is especially urgent to this day, as much of the risk for endometrial cancer development is influenced by the environment and lifestyle...
March 2018: Georgian Medical News
https://www.readbyqxmd.com/read/29675492/endometrial-mesenchymal-stem-stromal-cells-in-mature-and-immature-sheep-an-in-vitro-study
#3
Farnaz Ghobadi, Farhad Rahmanifar, Davood Mehrabani, Amin Tamadon, Mehdi Dianatpour, Shahrokh Zare, Iman Razeghian Jahromi
Background: Endometrial mesenchymal stem stromal cells (EnMSCs) are critical for uterine function, repair, and regeneration. Objective: This study introduced isolation technique of EnMSCs and compared the characteristics of EnMSCs in mature and immature ewes. Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes were collected from the slaughterhouse. Endometrial cells were isolated from tissue using cold incubation and then chopping and treating was performed with collagenase type I...
February 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29652725/therapeutic-strategies-involving-uterine-stem-cells-in-reproductive-medicine
#4
Michael Simoni, Hugh S Taylor
PURPOSE OF REVIEW: The current review provides an update on recent advances in stem cell biology relevant to female reproduction. RECENT FINDINGS: Stem cells are undifferentiated cells that often serve as a reservoir of cells to regenerate tissue in settings or injury or cell loss. The endometrium has progenitor stem cells that can replace all of the endometrium during each menstrual cycle. In addition, multipotent endometrial cells replace these progenitor cells when depleted...
June 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29604057/independent-development-of-endometrial-epithelium-and-stroma-within-the-same-endometriosis
#5
Michaël Noë, Ayse Ayhan, Tian-Li Wang, Ie-Ming Shih
The pathogenesis of endometriosis, a common benign but debilitating disease in women, remains elusive. The currently held stem cell theory posits that circulating progenitor/stem cells are deposited outside the uterus, where they differentiate into endometrial stroma and glandular tissue to establish endometriosis. Fundamental to testing this hypothesis is to elucidate the evolution of both tissue types. Here, we applied droplet digital PCR to analyze synonymous and missense somatic passenger mutations, which are neutral with respect to clonal selection, among six non-superficial endometriotic lesions...
March 31, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29571018/stem-cell-therapy-in-asherman-syndrome-and-thin-endometrium-stem-cell-based-therapy
#6
REVIEW
Ramyar Azizi, Leili Aghebati-Maleki, Mohammad Nouri, Faroogh Marofi, Sohrab Negargar, Mehdi Yousefi
The endometrium is one of the essential components of the uterus. The endometrium of human is a complex and dynamic tissue, which undergoes periods of growth and turn over during any menstrual cycle. Stem cells are initially undifferentiated cells that display a wide range of differentiation potential with no distinct morphological features. Stem cell therapy method recently has become a novel procedure for treatment of tissue injury and fibrosis in response to damage. Currently, there is massive interest in stem cells as a novel treatment method for regenerative medicine and more specifically for the regeneration of human endometrium disorder like Asherman syndrome (AS) and thin endometrium...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29546371/generation-of-mouse-for-conditional-expression-of-forkhead-box-a2
#7
Peng Wang, San-Pin Wu, Kelsey E Brooks, Andrew M Kelleher, Jessica J Milano-Foster, Francesco J DeMayo, Thomas E Spencer
Forkhead box A2 (FOXA2) is a pioneer transcription factor involved in organ development, function, and cancer. In the uterus, FOXA2 is essential for pregnancy and expressed specifically in the glands of the endometrium. Loss of FOXA2 function occurs during development of endometrial cancer in humans. The current study describes the development of a mouse model for conditional expression of mouse FOXA2. Using a system consisting of a minigene located at the Rosa26 locus, we generated a CAG-S-mFOXA2 allele in embryonic stem cells and subsequently in mice; before activation, the minigene is silent because of a floxed stop cassette inserted between the promoter and the transgene...
April 1, 2018: Endocrinology
https://www.readbyqxmd.com/read/29514724/oestrogen-progesterone-and-stem-cells-the-discordant-trio-in-endometriosis
#8
Chithra Janardhanan Susheelamma, Sathy M Pillai, Sivakumari Asha Nair
Oestrogen-progesterone signalling is highly versatile and critical for the maintenance of healthy endometrium in humans. The genomic and nongenomic signalling cascades initiated by these hormones in differentiated cells of endometrium have been the primary focus of research since 1920s. However, last decade of research has shown a significant role of stem cells in the maintenance of a healthy endometrium and the modulatory effects of hormones on these cells. Endometriosis, the growth of endometrium outside the uterus, is very common in infertile patients and the elusiveness in understanding of disease pathology causes hindrance in selection of treatment approaches to enhance fertility...
March 8, 2018: Expert Reviews in Molecular Medicine
https://www.readbyqxmd.com/read/29450941/hematogenous-dissemination-of-mesenchymal-stem-cells-from-endometriosis
#9
Fei Li, Myles H Alderman, Aya Tal, Ramanaiah Mamillapalli, Alexis Coolidge, Demetra Hufnagel, Zhihao Wang, Elham Neisani, Stephanie Gidicsin, Graciela Krikun, Hugh S Taylor
Endometriosis is ectopic growth of endometrial tissue traditionally thought to arise through retrograde menstruation. We aimed to determine if cells derived from endometriosis could enter vascular circulation and lead to hematogenous dissemination. Experimental endometriosis was established by transplanting endometrial tissue from DsRed+ mice into the peritoneal cavity of DsRed- mice. Using flow cytometry, we identified DsRed+ cells in blood of animals with endometriosis. The circulating donor cells expressed CXCR4 and mesenchymal stem cell (MSC) biomarkers, but not hematopoietic stem cell markers...
February 16, 2018: Stem Cells
https://www.readbyqxmd.com/read/29433563/therapeutic-effect-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-injured-rat-endometrium-during-its-chronic-phase
#10
Lu Zhang, Ying Li, Chun-Yi Guan, Shi Tian, Xiao-Dan Lv, Jian-Hui Li, Xu Ma, Hong-Fei Xia
BACKGROUND: Repair deficiency after endometrial injury is an important reason for intra-uterine adhesions, amenorrhea, and infertility in females. Bone marrow-derived mesenchymal stem cell (BMSC) transplantation is effective in repairing the damaged endometrium. However, the possibility of using umbilical cord-derived MSCs (UC-MSCs) to treat endometrial injury is rarely reported. METHODS: Ethanol (95%) was injected into rat uterus to establish a model of endometrial injury...
February 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29134714/identification-of-perivascular-and-stromal-mesenchymal-stem-progenitor-cells-in-porcine-endometrium
#11
J Wiater, M Niedziela, A Posmysz, K Wartalski, B Gajda, Z Smorąg, Z Rajfur, J Karasiński
Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels...
April 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28972492/age-related-specificities-of-endometrial-proliferation-stem-cell-index-distribution
#12
N Kikalishvili, R Beriashvili, T Muzashvili, G Burkadze
Endometrial stem cells take the special place among somatic stem cells of female reproductive system, since their essence and nature still remains unclear - cellular complexity of endometrium, its hormone-dependence and cycling remodeling makes the study of endometrial stem cells exclusively complicated. Consequently, the detection of endometrial stem cells and identification of their location in the complex cellular hierarchy still remains challenging. The aim of our study was to explore the age-related specificities of endometrial proliferative / stem cell index distribution under normal and hyperplasia conditions...
September 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28828000/microrna-expression-analysis-in-endometriotic-serum-treated-mesenchymal-stem-cells
#13
Mazen Abdel-Rasheed, Ghada Nour Eldeen, Marwa Mahmoud, Mahmoud ElHefnawi, Nourhan Abu-Shahba, Mohamed Reda, Khaled Elsetohy, Michael Nabil, Amr Elnoury, Tamer Taha, Osama Azmy
Endometriosis is defined by presence of endometrial-like-tissue outside the uterus. Recently, ectopic endometriotic lesions have been suggested to originate by abnormal differentiation of endometrial mesenchymal stem cells (eMSCs). MicroRNAs (miRNAs) play an important role in the pathophysiology of endometriosis. Through a PCR array approach, we aimed to assess the differential expression of microRNAs in human eMSC treated in culture with sera derived from women with severe endometriosis. Sera were collected from five patients with severe endometriosis and three control women and added individually in the culture medium to conduct experimental and control eMSC sets, respectively...
2017: EXCLI Journal
https://www.readbyqxmd.com/read/28782281/systemic-administration-of-bone-marrow-derived-cells-leads-to-better-uterine-engraftment-than-use-of-uterine-derived-cells-or-local-injection
#14
Ying Liu, Reshef Tal, Nicola Pluchino, Ramanaiah Mamillapalli, Hugh S Taylor
Stem cells are recruited to the uterus where they differentiate into endometrial cells and have been suggested as potential therapy for uterine injury such as Asherman's syndrome. However, it is unknown whether local intrauterine injection may result in better stem cell engraftment of the uterus compared with systemic administration, and whether uterine-derived cells (UDCs) may confer an advantage over BM-derived cells (BMDCs). Mice underwent local injury to a single uterine horn. Green fluorescent protein (GFP)-expressing BMDCs, UDCs or saline (control) were injected either intravenously or locally (uterine lumen) into wild-type recipients...
January 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28701175/isolation-and-characterization-of-equine-endometrial-mesenchymal-stromal-cells
#15
B Elisabeth Rink, Karin R Amilon, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
BACKGROUND: Equine mesenchymal stromal/stem cells (MSCs) are most commonly harvested from bone marrow (BM) or adipose tissue, requiring the use of surgical procedures. By contrast, the uterus can be accessed nonsurgically, and may provide a more readily available cell source. While human endometrium is known to harbor mesenchymal precursor cells, MSCs have not been identified in equine endometrium. This study reports the isolation, culture, and characterization of MSCs from equine endometrium...
July 12, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28578177/the-impact-of-uterine-immaturity-on-obstetrical-syndromes-during-adolescence
#16
Ivo Brosens, Joanne Muter, Caroline E Gargett, Patrick Puttemans, Giuseppe Benagiano, Jan J Brosens
Pregnant nulliparous adolescents are at increased risk, inversely proportional to their age, of major obstetric syndromes, including preeclampsia, fetal growth restriction, and preterm birth. Emerging evidence indicates that biological immaturity of the uterus accounts for the increased incidence of obstetrical disorders in very young mothers, possibly compounded by sociodemographic factors associated with teenage pregnancy. The endometrium in most newborns is intrinsically resistant to progesterone signaling, and the rate of transition to a fully responsive tissue likely determines pregnancy outcome during adolescence...
November 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28438190/very-small-embryonic-like-stem-cells-vsels-in-adult-mouse-uterine-perimetrium-and-myometrium
#17
Deepa Bhartiya, Kreema James
We have earlier reported the presence of very small embryonic-like stem cells (VSELs) in adult mouse uterus along with slightly bigger progenitors termed endometrial stem cells (EnSCs) and their regulation by ovarian hormones thus demonstrating a crucial role played by them during proliferation, differentiation and remodeling of the endometrium. Present study is a brief communication wherein we have examined the effect of higher dose of estrogen (E, 2 μg/day), progesterone (P, 1 mg/day) and follicle stimulating hormone (FSH, 5 IU/day for 5 days) specifically on the myometrium and perimetrium surrounding the endometrium in bilaterally ovariectomized mice...
April 24, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28357151/caprine-endometrial-mesenchymal-stromal-stem-cell-multilineage-potential-characterization-and-growth-kinetics-in-breeding-and-anestrous-stages
#18
Amin Tamadon, Davood Mehrabani, Younes Zarezadeh, Farhad Rahmanifar, Mehdi Dianatpour, Shahrokh Zare
The endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic cell lines as well as their growth kinetics in breeding and anestrous stages were evaluated. En-MSCs were enzymatically isolated from endometrial layer of the uterus of adult goats and were cultured and subcultured until passage 4...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/27821558/endometriosis-and-stem-cell-trafficking
#19
REVIEW
Nicola Pluchino, Hugh S Taylor
Adult stem cells have a major role in endometrial physiology, remodeling, and repair, but they also have a critical role in the development and progression of endometriosis. Bone marrow-derived stem cells (BMDSCs) engraft eutopic endometrium and endometriotic lesions, showing stromal and epithelial fate. Nevertheless, circulating BMDSCs are in limited supply, and the presence of endometriosis depletes stem cells from the blood circulation, preventing their homing in the uterus. Furthermore, stem cells migrate from endometriotic lesion into the uterus, leading to a dysfunctional endometrium...
December 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27729562/bone-marrow-stem-cell-chemotactic-activity-is-induced-by-elevated-cxcl12-in-endometriosis
#20
Irene Moridi, Ramanaiah Mamillapalli, Emine Cosar, Gulcin Sahin Ersoy, Hugh S Taylor
Endometriosis is an inflammatory gynecological disorder caused by the growth of endometrial tissue outside the uterus. Endometriosis produces chemokines, including CXCL12, that attract bone marrow cells to the lesions. In this study, we describe the expression, localization, and chemotactic activity of CXCL12 in endometriotic lesions. Biopsies were collected both from women with endometriosis undergoing laparoscopy and control endometrium from women without endometriosis. Expression of CXCl12 and CXCR4 messenger RNA was increased approximately 4- and 6-fold, respectively, in endometriosis compared to eutopic endometrium...
April 2017: Reproductive Sciences
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