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https://www.readbyqxmd.com/read/29679941/wood-and-bark-of-pinus-halepensis-as-archives-of-heavy-metal-pollution-in-the-mediterranean-region
#1
José Antonio Rodríguez Martin, Carmen Gutiérrez, Manuel Torrijos, Nikos Nanos
Natural levels of heavy metals (HM) have increased during the industrial era to the point of posing a serious threat to the environment. The use of tree species to record contamination is a well-known practice. The objective of the study was to compare HM levels under different pollution conditions: a) soil pollution due to mining waste; b) atmospheric pollution due to coal-fired power plant emissions. We report significant HM enrichment in Pinus halepensis tissues. Near a burning power plant, Pb content in a tree wood was 2...
April 18, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29679873/disentangling-the-mental-health-impact-of-childhood-abuse-and-neglect-a-replication-and-extension-study-in-a-brazilian-sample-of-high-risk-youth
#2
Irismar Reis de Oliveira, Ana Cristina Matos-Ragazzo, Yuning Zhang, Nina Maia Vasconcelos, Michella Lopes Velasquez, Daniela Reis, Monica Gonçalves Ribeiro, Marina Monzani da Rocha, Maria Conceição Rosario, Paul Stallard, Charlotte A M Cecil
Childhood maltreatment is a key predictor of mental health problems across the life span. Yet, how maltreatment types independently and jointly influence the risk for psychiatric problems remains unclear. The aim of the study was two-fold: first, to replicate recent findings regarding the impact of maltreatment types on youth psychiatric symptoms, based on a Brazilian sample of high-risk adolescents (n = 347; age range = 11-17 yrs), and second, to extend existing findings by examining whether this relationship is mediated by bullying victimization and/or perpetration...
April 18, 2018: Child Abuse & Neglect
https://www.readbyqxmd.com/read/29679865/generation-and-characterization-of-hagfish-variable-lymphocyte-receptor-b-against-glycoprotein-of-viral-hemorrhagic-septicemia-virus-vhsv
#3
Jung Seok Lee, Jaesung Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jae Wook Jung, Tae Won Gong, Young Rim Kim, Jeong Ho Lee, Hyoung Jun Kim, Tae Sung Jung
Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (Eptatretus burgeri) and lamprey (Petromyzon marinus). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates via the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail...
April 18, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29679846/biodegradability-and-ecological-safety-assessment-of-stenotrophomonas-sp-ddt-1-in-the-ddt-contaminated-soil
#4
Hua Fang, Yanfei Deng, Qiqing Ge, Jiajia Mei, Houpu Zhang, Huifang Wang, Yunlong Yu
The biodegradability and ecological safety assessment of the previously isolated DDT-degrading bacterial strain Stenotrophomonas sp. DDT-1 were investigated in the DDT-contaminated soil under laboratory and field conditions. Under laboratory conditions, the degradation rates of fresh p,p'-DDT in soil were enhanced by 2.0-3.0-fold with the introduction of the strain DDT-1 compared to those of the control treatments. A similar enhancement in the dissipation of DDTs (p,p'-DDT, p,p'-DDE, p,p'-DDD, and o,p'-DDT) in the aged DDT-contaminated field plot soils resulted from the inoculation with this strain...
April 18, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29679814/prediction-of-recurrent-ectopic-pregnancy-a-five-year-follow-up-cohort-study
#5
Mohamed Ellaithy, Mufareh Asiri, Ahmed Rateb, Ahmed Altraigey, Khaled Abdallah
OBJECTIVE: To evaluate the value of different clinical risk factors in the prediction of recurrent ectopic pregnancies (REP). STUDY DESIGN: This cohort study was carried out at a tertiary-care maternity hospital in Saudi Arabia, between January 2010 and June 2017. All women with a primary ectopic pregnancy (EP) were consecutively recruited, thoroughly assessed and followed up for a minimum duration of five-years (5.6 ± 0.61 years) to diagnose the nature and location of the subsequent pregnancies...
April 10, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29679799/refolding-kinetics-of-cytochrome-c-studied-with-microsecond-timescale-continuous-flow-uv-vis-spectroscopy-and-rapid-freeze-quench-epr
#6
Batoul Srour, Marc J F Strampraad, Wilfred R Hagen, Peter-Leon Hagedoorn
The study of the structure, function, folding and conformational transitions of cytochrome c is of great interest because this protein plays an important role in biological electron transport and apoptosis. The different native and non-native conformations have been studied extensively under equilibrium conditions at different pH values, however, kinetic studies are rare because they require technically challenging rapid mixing and spectroscopic monitoring techniques. Here we present the refolding kinetics of acid denatured cytochrome c using the pH jump technique from pH 2 to pH 4...
April 11, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29679798/liposomal-formulations-of-magnesium-sulfanyl-tribenzoporphyrazines-for-the-photodynamic-therapy-of-cancer
#7
Jaroslaw Piskorz, Dariusz T Mlynarczyk, Wojciech Szczolko, Krystyna Konopka, Nejat Düzgüneş, Jadwiga Mielcarek
Photodynamic therapy of cancer comprises the activation of photosensitizer molecules delivered to cancer cells, to generate reactive oxygen species that mediate cytotoxicity. In this study, previously synthesized dendritic magnesium tribenzoporphyrazines were incorporated into four types of liposomes containing either 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as the zwitterionic lipids. The addition of either l-α-phosphatidyl-dl-glycerol (PG) or 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) imparted a negative or positive charge, respectively...
April 9, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29679729/a-longitudinal-study-of-disc-height-narrowing-and-facet-joint-osteoarthritis-at-the-thoracic-and-lumbar-spine-evaluated-by-computed-tomography-the-framingham-study
#8
Mohamed Jarraya, Ali Guermazi, Amanda L Lorbergs, Elana Brochin, Douglas P Kiel, Mary L Bouxsein, L Adrienne Cupples, Elizabeth J Samelson
BACKGROUND CONTEXT: Prevalence and progression of disc height narrowing (DHN) and facet joint osteoarthritis (FJOA) in the thoracic and lumbar regions in non-clinical populations are not well-established. PURPOSE: Use computed tomography (CT) images to determine prevalence and progression of DHN and FJOA according to age, sex, and spinal region. STUDY DESIGN: Six-year longitudinal study SAMPLE: 1,196 members of the Framingham Study (mean baseline age 61 ±9 years) OUTCOME MEASURES: We compared prevalence and progression (new or worsening) of moderate-to-severe DHN and FJOA by age, sex, and spinal region...
April 18, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29679725/achyranthis-bidentatae-radix-enhanced-articular-distribution-and-anti-inflammatory-effect-of-berberine-in-sanmiao-wan-using-an-acute-gouty-arthritis-rat-model
#9
Juan Wu, Jingya Li, Wei Li, Bei Sun, Jin Xie, Wenming Cheng, Qunlin Zhang
ETHNOPHARMACOLOGICAL RELEVANCE: Sanmiao Wan (SMW) has been a basic prescription employed for the treatment for gout in the clinic since Yuan dynasty. Achyranthis bidentatae radix (ABR) is designed as a lower-guiding drug in SMW to augment the articular accumulation of active ingredients and improve the anti-inflammatory effect. AIM OF THE STUDY: Present study was undertaken to investigate the dose-response relationship of berberine in SMW between the articular concentration and anti-inflammatory effect in the knee joint under the lower-guiding of ABR...
April 18, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29679663/steric-shielding-protected-and-acidity-activated-pop-up-of-ligand-for-tumor-enhanced-photodynamic-therapy
#10
Kai Han, Zhaoyu Ma, Xinxin Dai, Jin Zhang, Heyou Han
Tumor targeted drug delivery in vivo remains a significant challenge in tumor therapy. In this article, we fabricated a steric shielding protected/tumor acidity-activated chimeric peptide for tumor targeted photodynamic therapy. This amphiphilic chimeric peptide could form spherical nanoparticles at neutrally physiological environment with the shielding of biotin molecule (tumor target ligand). When in tumor acidic microenvironment, acidity-sensitive dimethylmaleic amide was rapidly hydrolyzed, resulting in subsequent liberation of (Lys)8 and the recovery of intramolecular electrostatic interaction between (Lys)8 and (Glu)8 ...
April 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29679607/process-intensification-of-core-streptavidin-production-through-high-cell-density-cultivation-of-recombinant-e-coli-and-a-temperature-based-refolding-method
#11
Leong Huat Chua, Soo Choon Tan, Mervyn W O Liew
An intensified process was developed that enables high level production of recombinant core streptavidin (cSAV), a non-glycosylated tetrameric protein utilised in a wide range of applications. A pH-stat fed-batch feeding strategy was employed to achieve high-cell-density and improve volumetric yield of cSAV which was expressed as inclusion bodies (IBs). The effect of induction at different cell densities (OD 20, 60 and 100) on volumetric and specific yield were then studied. Highest volumetric yield of cSAV (1550 mg L-1 ) was obtained from induction at OD 100 without significant reductions in specific yield...
April 18, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29679584/remember-the-vessels-craniofacial-fracture-predicts-risk-for-blunt-cerebrovascular-injury
#12
Elina Varjonen, Frank Bensch, Tuomo Pyhältö, Mika Koivikko, Johanna Snäll
PURPOSE: The risk factors for blunt cerebrovascular injuries (BCVIs) are currently under intensive research, yet it is still controversial who should be screened. This study aimed to determine whether craniofacial fractures are associated with BCVI. PATIENTS AND METHODS: This retrospective cohort study focused on patients with suspected polytrauma after whole-body computed tomographic angiography of the cervical arteries. Patients were reviewed for BCVI and craniofacial fractures...
March 28, 2018: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29679572/a-novel-strategy-to-improve-the-thermostability-of-penicillium-camembertii-mono-and-di-acylglycerol-lipase
#13
Yanhua Liu, Dongjuan Yuan, Zexin Zhao, Dongming Lan, Bo Yang, Yonghua Wang
Penicillium camembertii (PCL), a mono- and di-acylglycerol lipase (DGL), has the vital potential in the oil chemistry for food industry. However, known DGLs are mesophilic enzymes which restricts its application in the industry. To improve thermostability of PCL, we used amino acid substitution by comparison of amino acids compositions of PCL and protein sequences from typical thermophilic bacteria. Then, some conservative residues around active center were avoided to mutate according to homologous alignment analyses...
April 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29679560/developmental-origin-and-morphogenesis-of-the-diaphragm-an-essential-mammalian-muscle
#14
Elizabeth M Sefton, Mirialys Gallardo, Gabrielle Kardon
The diaphragm is a mammalian skeletal muscle essential for respiration and for separating the thoracic and abdominal cavities. Development of the diaphragm requires the coordinated development of muscle, muscle connective tissue, tendon, nerves, and vasculature that derive from different embryonic sources. However, defects in diaphragm development are common and the cause of an often deadly birth defect, Congenital Diaphragmatic Hernia (CDH). Here we comprehensively describe the normal developmental origin and complex spatial-temporal relationship between the different developing tissues to form a functional diaphragm using a developmental series of mouse embryos genetically and immunofluorescently labeled and analyzed in whole mount...
April 18, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29679556/a-novel-smac-mimetic-apg-1387-exhibited-dual-antitumor-effect-on-hbv-positive-hepatocellular-carcinoma-with-high-expression-of-ciap2-by-inducing-apoptosis-and-enhancing-innate-anti-tumor-immunity
#15
Wentao Pan, Qiuyun Luo, Xianglei Yan, Luping Yuan, Hanjie Yi, Lin Zhang, Baoxia Li, Yuxin Zhang, Jian Sun, Miao-Zhen Qiu, Da-Jun Yang
Check point inhibitor anti-PD1 antibody produced some efficacy in Hepatocellular Carcinoma (HCC) patients previously treated with sorafenib. Unfortunately, HCC patients with hepatitis B virus (HBV) infection did not respond as well as uninfected patients. Previously, SMAC mimetics-the antagonist for inhibitor of apoptosis proteins (IAPs) can rapidly reduce serum hepatitis B virus DNA in animal model. APG-1387 is a novel SMAC-mimetic, small molecule inhibitor targeting inhibitor of apoptosis proteins (IAPs)...
April 18, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29679354/low-serum-phosphate-is-associated-with-ischemic-stroke-in-hemodialysis-patients
#16
Tsang-Shan Chen, Chao-Hung Chen, Chien-An Chen, Li-Ping Chou, Chi-Chu Liu
BACKGROUND: Hemodialysis patients have a high incidence of ischemic stroke. However, the association between serum phosphate levels and ischemic stroke is controversial among hemodialysis patients. The present study is used to evaluate whether serum phosphate levels are associated with ischemic stroke among patients undergoing hemodialysis. METHODS: A total of 84 hemodialysis patients were followed up for 8 years. Data collection included chart reviews and assessments of laboratory records...
April 20, 2018: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/29679312/efficient-production-of-secretory-streptomyces-clavuligerus-%C3%AE-lactamase-inhibitory-protein-blip-in-pichia-pastoris
#17
Kin-Ho Law, Man-Wah Tsang, Yuk-Ki Wong, Ming-San Tsang, Pui-Yee Lau, Kwok-Yin Wong, Kwok-Ping Ho, Yun-Chung Leung
β-Lactamase inhibitory protein (BLIP), a low molecular weight protein from Streptomyces clavuligerus, has a wide range of potential applications in the fields of biotechnology and pharmaceutical industry because of its tight interaction with and potent inhibition on clinically important class A β-lactamases. To meet the demands for considerable amount of highly pure BLIP, this study aimed at developing an efficient expression system in eukaryotic Pichia pastoris (a methylotrophic yeast) for production of BLIP...
April 20, 2018: AMB Express
https://www.readbyqxmd.com/read/29679302/association-of-abnormal-serum-potassium-levels-with-arrhythmias-and-cardiovascular-mortality-a-systematic-review-and-meta-analysis-of-observational-studies
#18
REVIEW
Liesa K Hoppe, Dana C Muhlack, Wolfgang Koenig, Prudence R Carr, Hermann Brenner, Ben Schöttker
PURPOSE: To provide the first systematic review and meta-analysis of observational studies on the association of abnormal serum potassium and cardiovascular outcomes. METHODS: Medline and ISI Web of Knowledge were systematically searched from inception until November 24, 2017. Data synthesis of relevant studies was performed using random effects model meta-analyses. RESULTS: Meta-analyses included 310,825 participants from 24 studies. In the older general population, low serum potassium was associated with a 1...
April 20, 2018: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/29679159/notch-1-sirna-and-methotrexate-towards-a-multifunctional-approach-in-rhematoid-arthritis-management-a-nanomedicine-approach
#19
Guang Zhao, Haifei Zhang
PURPOSE: Rheumatoid arthritis (RA) is a chronic inflammatory disease and Notch pathway plays a pivotal role in synoviocytes involved in progression of RA. METHODS: Herein, we have designed a self-assembled polymeric micelles based on polycaprolactone-polyethylene glycol (PCL-PEG) and polyethylenimine-polyethylene glycol (PEI-PEG) was prepared and loaded with methotrexate and Notch-1 siRNA for the effective treatment of rheumatoid arthritis. RESULTS: The MTX/siRNA-loaded polymeric micelles (siM-PM) showed appreciable cellular uptake in Raw264...
April 20, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29679034/enteric-nervous-system-regulation-of-intestinal-stem-cell-differentiation-and-epithelial-monolayer-function
#20
Marissa Puzan, Sanjin Hosic, Caroline Ghio, Abigail Koppes
The Enteric Nervous System (ENS) is a complex network of neurons and glia, which regulates sensorimotor function throughout the gastroinestinal tract (GI). Here we investigated the role of the ENS and intestinal myofibroblasts in the maintenance of a primary intestinal epithelial barrier through regulation of monolayer permeability, cytokine production, and differentiation of intestinal stem cells. Utilizing a novel, in vitro, transwell-based coculture system, murine small intestinal stem cells were isolated and cultured with ENS neurons and glia or subepithelial myofibroblasts...
April 20, 2018: Scientific Reports
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