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https://www.readbyqxmd.com/read/28651368/risk-of-recurrence-in-pituitary-neuroendocrine-tumors-a-prospective-study-using-a-five-tiered-classification
#1
Gérald Raverot, Emmanuelle Dantony, Julie Beauvy, Alexandre Vasiljevic, Sara Mikolasek, Françoise Borson-Chazot, Emmanuel Jouanneau, Pascal Roy, Jacqueline Trouillas
Background: Most pituitary neuroendocrine tumors (PitNET) show benign behavior, but a significant number are invasive, recur or resist to medical treatment. Based on a retrospective case-control study we recently proposed a classification of PitNETs of prognostic relevance. This prospective study aims to test the value of this classification in an independent patient cohort. Methods: The cohort included patients of a single center operated upon a PitNET. Using a grading system based on invasion on MRI, immunocytochemical profile (ICC), Ki-67, mitotic index, and p53 positivity, tumors were classified...
June 23, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28651352/circular-rna-profiling-reveals-that-circular-rnas-from-anxa2-can-be-used-as-new-biomarkers-for-multiple-sclerosis
#2
Iparraguirre Leire, Muñoz-Culla Maider, Prada Iñigo, Castillo-Triviño Tamara, Olascoaga Javier, Otaegui David
Multiple Sclerosis is an autoimmune disease, with higher prevalence in women, in whom the immune system is dysregulated. This dysregulation has been shown to correlate with changes in transcriptome expression as well as in gene-expression regulators, such as non-coding RNAs (e.g. microRNAs). Indeed, some of these have been suggested as biomarkers for multiple sclerosis even though few biomarkers have reached the clinical practice.Recently, a novel family of non-coding RNAs, circular RNAs, has emerged as a new player in the complex network of gene-expression regulation...
June 23, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28651320/an-efficient-production-of-high-pure-xylooligosaccharides-from-corncob-with-affinity-adsorption-enzymatic-reaction-integrated-approach
#3
Siyuan Chang, Jianlin Chu, Yalan Guo, Huo Li, Bin Wu, Bingfang He
Xylooligosaccharides (XOS) are high value-added ingredients for functional foods and they have potential use as prebiotics. In order to reduce the production cost of XOS, we constructed a fusion enzyme consisting of expansin and endo-xylanase from B. subtilis Lucky9 to produce high-pure XOS with affinity adsorption-enzymatic reaction integrated approach. By optimization of inserting linker, the specific xylanase activity of fusion enzyme with R2 linker was increased 1.28-fold than that of native xylanase. Electrophoretic pure fusion enzyme was separated from crude enzyme extracts by affinity adsorption on corncob substrate, with 90...
June 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651197/ascorbic-acid-protects-coccomyxa-subellipsoidea-against-metal-toxicity-through-modulation-of-ros-no-balance-and-metal-uptake
#4
Jozef Kováčik, Gabriela Rotková, Marek Bujdoš, Petr Babula, Viera Peterková, Peter Matúš
Impact of exogenous ascorbic acid (AsA, 100μM) on acute metal toxicity (Pb and Hg, 24h of exposure to 100μM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced...
June 16, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28651187/association-of-ectopic-fat-with-abdominal-aorto-illiac-and-coronary-artery-calcification-in-african-ancestry-men
#5
Allison L Kuipers, Joseph M Zmuda, J Jeffrey Carr, James G Terry, Sangeeta Nair, Ryan Cvejkus, Clareann H Bunker, Alan L Patrick, Christina L Wassel, Iva Miljkovic
BACKGROUND AND AIMS: There is strong evidence that fat accumulating in non-adipose sites, "ectopic fat", is associated with cardiovascular disease (CVD), including vascular calcification. Most previous studies of this association have assessed only a single ectopic fat depot. Therefore, our aim was to assess the association of total, regional, and ectopic fat with abdominal aorto-illiac calcification (AAC) and coronary artery calcification (CAC) in 798 African ancestry men. METHODS: Participants (mean age 62) were from the Tobago Bone Health Study cohort...
June 16, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28651167/error-prone-bypass-of-o-6-methylguanine-by-dna-polymerase-of-pseudomonas-aeruginosa-phage-pap1
#6
Shiling Gu, Jingyuan Xiong, Ying Shi, Jia You, Zhenyu Zou, Xiaoying Liu, Huidong Zhang
O(6)-Methylguanine (O(6)-MeG) is highly mutagenic and is commonly found in DNA exposed to methylating agents, generally leads to G:C to A:T mutagenesis. To study DNA replication encountering O(6)-MeG by the DNA polymerase (gp90) of P. aeruginosa phage PaP1, we analyzed steady-state and pre-steady-state kinetics of nucleotide incorporation opposite O(6)-MeG by gp90 exo(-). O(6)-MeG partially inhibited full-length extension by gp90 exo(-). O(6)-MeG greatly reduces dNTP incorporation efficiency, resulting in 67-fold preferential error-prone incorporation of dTTP than dCTP...
June 10, 2017: DNA Repair
https://www.readbyqxmd.com/read/28651156/alterations-in-aspergillus-brasiliensis-niger-atcc-9642-membranes-associated-to-metabolism-modifications-during-application-of-low-intensity-electric-current
#7
Nancy Velasco-Alvarez, Mariano Gutiérrez-Rojas, Ignacio González
The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated with A. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30°C). Then, 4.5days after starting the culture, a current of 0.42mAcm(-2) was applied for 24h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4...
June 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28651101/competitive-ligands-facilitate-dissociation-of-the-complex-of-bifunctional-inhibitor-and-protein-kinase
#8
Taavi Ivan, Erki Enkvist, Hedi Sinijarv, Asko Uri
Dissociation of the complex of a ligand and a protein usually follows the kinetic profile of the first order process and the rate of dissociation is not affected by the presence of competitive ligands. We discovered that dissociation of the complex between a bifunctional ligand and a protein kinase (the catalytic subunit of cAMP-dependent protein kinase), an enzyme possessing 2 different substrate binding sites, was accelerated (facilitated) over 50-fold in the presence of competitive ligands at higher concentrations...
June 20, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28651082/impact-of-humic-acid-on-the-degradation-of-levofloxacin-by-aqueous-permanganate-kinetics-and-mechanism
#9
Ke Xu, Weiwei Ben, Wencui Ling, Yu Zhang, Jiuhui Qu, Zhimin Qiang
Levofloxacin (LF) is a frequently detected fluoroquinolone in surface water, and permanganate (MnO4(-)) is a commonly used oxidant in drinking water treatment. This study investigated the impact of humic acid (HA) on LF degradation by aqueous MnO4(-) from both kinetic and mechanistic aspects. In the absence of HA, the second-order rate constant (k) of LF degradation by MnO4(-) was determined to be 3.9 M(-1) s(-1) at pH 7.5, which increased with decreasing pH. In the presence of HA, the pseudo-first-order rate constant (kobs) of LF degradation at pH 7...
June 14, 2017: Water Research
https://www.readbyqxmd.com/read/28651056/significantly-improved-protein-folding-thermodynamics-using-a-dispersion-corrected-water-model-and-a-new-residue-specific-force-field
#10
Hao-Nan Wu, Fan Jiang, Yun-Dong Wu
An accurate potential energy model is crucial for biomolecular simulations. Despite many recent improvements of classical protein force fields, there are remaining key issues: much weaker temperature-dependence of folding/unfolding equilibrium, and overly collapsed unfolded or disordered states. For the later problem, a new water model (TIP4P-D) has been proposed to correct the significantly underestimated water dispersion interactions. Here, using TIP4P-D, we reveal problems in current force fields through failures in folding model systems (a polyalanine peptide, Trp-cage and the GB1 hairpin)...
June 26, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28651051/molecular-precision-at-micrometer-length-scales-hierarchical-assembly-of-dna-protein-nanostructures
#11
Daniel Schiffels, Veronika A Szalai, J Alexander Liddle
Robust self-assembly across length scales is a ubiquitous feature of biological systems, but remains challenging for synthetic structures. Taking a cue from biology - where disparate molecules work together to produce large, functional assemblies - we demonstrate how to engineer microscale structures with nanoscale features: Our self-assembly approach begins by using DNA polymerase to controllably create double-stranded DNA (dsDNA) sections on a single-stranded template. The single-stranded DNA (ssDNA) sections are then folded into a mechanically flexible skeleton by the origami method...
June 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28651022/can-natural-variation-in-grain-p-concentrations-be-exploited-in-rice-breeding-to-lower-fertilizer-requirements
#12
Fanmiao Wang, James Douglas Morrison King, Terry Rose, Tobias Kretzschmar, Matthias Wissuwa
Agricultural usage of phosphorus (P) is largely driven by the amount of P removed from fields in harvested plant matter as offtake needs to be balanced by P fertilizer application. Reducing P concentration in grains is a way to decrease P offtake and reduce P fertilizer requirements or soil P mining where insufficient P is applied. Our objective was to assesses the genotypic variation for grain P concentration present within the rice gene pool and resolve to what extent it is affected by environment (P supply) or associated with genetic factors...
2017: PloS One
https://www.readbyqxmd.com/read/28651013/loss-of-prion-protein-induces-a-primed-state-of-type-i-interferon-responsive-genes
#13
Giulia Malachin, Malin R Reiten, Øyvind Salvesen, Håvard Aanes, Jorke H Kamstra, Kerstin Skovgaard, Peter M H Heegaard, Cecilie Ersdal, Arild Espenes, Michael A Tranulis, Maren K Bakkebø
The cellular prion protein (PrPC) has been extensively studied because of its pivotal role in prion diseases; however, its functions remain incompletely understood. A unique line of goats has been identified that carries a nonsense mutation that abolishes synthesis of PrPC. In these animals, the PrP-encoding mRNA is rapidly degraded. Goats without PrPC are valuable in re-addressing loss-of-function phenotypes observed in Prnp knockout mice. As PrPC has been ascribed various roles in immune cells, we analyzed transcriptomic responses to loss of PrPC in peripheral blood mononuclear cells (PBMCs) from normal goat kids (n = 8, PRNP+/+) and goat kids without PrPC (n = 8, PRNPTer/Ter) by mRNA sequencing...
2017: PloS One
https://www.readbyqxmd.com/read/28651008/production-and-purification-of-human-hsp90%C3%AE-in-escherichia-coli
#14
Martina Radli, Dmitry B Veprintsev, Stefan G D Rüdiger
The molecular chaperone Hsp90 is an essential member of the cellular proteostasis system. It plays an important role in the stabilisation and activation of a large number of client proteins and is involved in fatal disease processes, e.g. Alzheimer disease, cancer and cystic fibrosis. This makes Hsp90 a crucial protein to study. Mechanistic studies require large amounts of protein but the production and purification of recombinant human Hsp90 in Escherichia coli is challenging and laborious. Here we identified conditions that influence Hsp90 production, and optimised a fast and efficient purification protocol...
2017: PloS One
https://www.readbyqxmd.com/read/28651003/iron-elevation-and-adipose-tissue-remodeling-in-the-epididymal-depot-of-a-mouse-model-of-polygenic-obesity
#15
Xiaoya Ma, Vinh T Pham, Hiroyuki Mori, Ormond A MacDougald, Yatrik M Shah, Peter F Bodary
BACKGROUND: Iron dysregulation is a potential contributor to the pathology of obesity-related metabolic complications. KK/HIJ (KK) mice, a polygenic obese mouse model, have elevated serum iron levels. A subset of KK male mice display a bronzing of epididymal adipose tissue (eAT) associated with >100-fold (p<0.001) higher iron concentration. METHODS: To further phenotype and characterize the adipose tissue iron overload, 27 male KK mice were evaluated. 14 had bronzing eAT and 13 had normal appearing eAT...
2017: PloS One
https://www.readbyqxmd.com/read/28650973/interferon-signaling-in-peromyscus-leucopus-confers-a-potent-and-specific-restriction-to-vector-borne-flaviviruses
#16
Adaeze O Izuogu, Kristin L McNally, Stephen E Harris, Brian H Youseff, John B Presloid, Christopher Burlak, Jason Munshi-South, Sonja M Best, R Travis Taylor
Tick-borne flaviviruses (TBFVs), including Powassan virus and tick-borne encephalitis virus cause encephalitis or hemorrhagic fevers in humans with case-fatality rates ranging from 1-30%. Despite severe disease in humans, TBFV infection of natural rodent hosts has little noticeable effect. Currently, the basis for resistance to disease is not known. We hypothesize that the coevolution of flaviviruses with their respective hosts has shaped the evolution of potent antiviral factors that suppress virus replication and protect the host from lethal infection...
2017: PloS One
https://www.readbyqxmd.com/read/28650961/mfpred-rapid-and-accurate-prediction-of-protein-peptide-recognition-multispecificity-using-self-consistent-mean-field-theory
#17
Aliza B Rubenstein, Manasi A Pethe, Sagar D Khare
Multispecificity-the ability of a single receptor protein molecule to interact with multiple substrates-is a hallmark of molecular recognition at protein-protein and protein-peptide interfaces, including enzyme-substrate complexes. The ability to perform structure-based prediction of multispecificity would aid in the identification of novel enzyme substrates, protein interaction partners, and enable design of novel enzymes targeted towards alternative substrates. The relatively slow speed of current biophysical, structure-based methods limits their use for prediction and, especially, design of multispecificity...
June 26, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28650865/effect-of-obesity-on-bladder-cancer-and-renal-cell-carcinoma-incidence-and-survival
#18
Philipp Gild, Behfar Ehdaie, Luis A Kluth
PURPOSE OF REVIEW: The prevalence of obesity has risen dramatically in the general population and among cancer survivors in the last three decades. In this review, we highlight the impact of obesity on carcinogenesis and survival with a focus on bladder cancer and renal cell carcinoma (RCC). RECENT FINDINGS: Obesity presents an established risk factor for an up to 1.8-fold relative risk of RCC. Data with regard to bladder cancer are less abundant but support this association as well...
June 24, 2017: Current Opinion in Urology
https://www.readbyqxmd.com/read/28650778/success-of-dental-treatments-under-behavior-management-sedation-and-general-anesthesia
#19
Sigalit Blumer, Liora Costa, Benjamin Peretz
OBJECTIVE: To present comparative study aims to assist the practitioner to choose between behavior modification (BM) techniques, pharmacologic sedation (N2O-O2 alone or combined with midazolam 0.5 mg/ kg) or routine general anesthesia (GA) for the most successful approach in enabling pediatric dental care. STUDY DESIGN: Dental records of 56 children treated in a university dental clinic between 2006-2016 were reviewed, and data on age, gender, required treatment (amalgam restorations, composite restorations, pulpotomy, and stainless steel crowns [SSC]), treatment approaches and therapeutic success at final follow-up were retrieved...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28650775/urine-interleukin-18-il-18-as-a-biomarker-of-total-body-irradiation-a-preliminary-study-in-nonhuman-primates
#20
Mang Xiao, David L Bolduc, XiangHong Li, Wanchang Cui, Kevin P Hieber, Rolf Bünger, Natalia I Ossetrova
We have reported that circulating IL-18 can be used as a radiation biomarker in mice, minipigs and nonhuman primates (NHPs, Macaca mulatta). Here, we report the levels of IL-18 in individual NHP's urine before and at 6 h-7 days after 5.0, 6.5 and 8.5 Gy (60)Co total-body irradiation (TBI) using enzyme linked immunosorbent assay (ELISA). Six animals (3.5-5.5 kg, 3-4 years old) per radiation dose were investigated. Correlation values between urine IL-18 and blood cell counts and serum chemistry parameters including lactate dehydrogenase (LDH), lipase, and serum total protein (TP), as well as between urine IL-18 and 60-day survival, were analyzed...
June 26, 2017: Radiation Research
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