keyword
MENU ▼
Read by QxMD icon Read
search

Nuclear deformation

keyword
https://www.readbyqxmd.com/read/29156519/in-vivo-assessment-of-the-osteo-protective-effects-of-eugenol-in-alveolar-bone-tissues
#1
Hatem M Abuohashish, Dina A Khairy, Maha M Abdelsalam, Ahmed Alsayyah, Mohammed M Ahmed, Salim S Al-Rejaie
Estrogen deficiency following menopausal provokes alveolar bone loss, remodeling and inflammation. Eugenol is a phenolic compound with wide dental applications and anti-inflammatory properties. In the present study, the potential protective role of eugenol against alveolar bone deformities was investigated in an ovariectomized (OVX) rodent model. Two doses of eugenol (2.5 and 5 mg/kg/d) were administered to OVX animals for 12 weeks. In Serum, markers of bone metabolism and pro-inflammatory cytokines were estimated using ELISA...
November 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29150809/molecular-properties-of-metal-difluorides-and-their-interactions-with-co2-and-h2o-molecules-a-dft-investigation
#2
Agnes Lincy Arokiyanathan, Senthilkumar Lakshmipathi
A computational study of metal difluorides (MF2; M = Ca to Zn) and their interactions with carbon dioxide and water molecules was performed. The structural parameter values obtained and the results of AIM analysis and energy decomposition analysis indicated that the Ca-F bond is weaker and less ionic than the bonds in the transition metal difluorides. A deformation density plot revealed the stablizing influence of the Jahn-Teller effect in nonlinear MF2 molecules (e.g., where M= Sc, Ti, Cr). An anaysis of the metal K-edge peaks of the difluorides showed that shifts in the edge energy were due to the combined effects of the ionicity, effective nuclear charge, and the spin state of the metal...
November 18, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29126229/osteoblast-amp-activated-protein-kinase-regulates-postnatal-skeletal-development-in-male-mice
#3
Ippei Kanazawa, Ayumu Takeno, Ken-Ichiro Tanaka, Masakazu Notsu, Toshitsugu Sugimoto
Studies have shown that AMP-activated protein kinase (AMPK), a crucial regulator of energy homeostasis, plays important roles in osteoblast differentiation and mineralization. However, little is known about in vivo roles of osteoblastic AMPK in bone development. Thus, to investigate in vivo roles of osteoblast AMPK, we conditionally inactivated Ampk in osterix (Osx)-expressing cells by crossing Osx-Cre mice with floxed AMPKα1 to generate mice lacking AMPKα1 in osteoblasts (Ampk-/- mice). Compared with wild-type and Ampk+/- mice, Ampk-/- mice displayed retardation of postnatal bone development, although bone deformity was not observed at birth...
November 3, 2017: Endocrinology
https://www.readbyqxmd.com/read/29119545/mechanisms-of-nuclear-pore-complex-assembly-two-different-ways-of-building-one-molecular-machine
#4
REVIEW
Shotaro Otsuka, Jan Ellenberg
The nuclear pore complex (NPC) mediates all macromolecular transport across the nuclear envelope. In higher eukaryotes that have an open mitosis, NPCs assemble at two points in the cell-cycle: during nuclear assembly in late mitosis and during nuclear growth in interphase. How the NPC, the largest non-polymeric protein complex in eukaryotic cells, self-assembles inside cells remained unclear. Recent studies have started to uncover the assembly process, and evidence has been accumulating that postmitotic and interphase NPC assembly use fundamentally different mechanisms; the duration, structural intermediates, and regulation by molecular players are different and different types of membrane deformation are involved...
November 8, 2017: FEBS Letters
https://www.readbyqxmd.com/read/29119279/mechanical-stress-promotes-matrix-synthesis-of-mandibular-condylar-cartilage-via-the-rkip-erk-pathway
#5
Lian Sun, Jing Zhao, Hua Wang, Yongchu Pan, Lin Wang, Wei-Bing Zhang
Mandibular hypoplasia is a common jaw deformity that affects breathing, occlusal function and facial aesthetics. Stimulating mandibular condylar growing with functional appliances is an ordinary but controversial treatment method in orthodontics. Therefore, it is vital to clarify how functional appliances affect condylar growing. Raf-1 kinase inhibitor protein (RKIP), as an endogenous inhibitory molecule of the ERK signaling, is postulated to involve in stress-induced response to articular cartilage. This study was to reveal the role of RKIP in regulating cartilage matrix synthesis with functional appliance treatment...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29112878/reproductive-and-endocrine-disrupting-toxicity-of-microcystis-aeruginosa-in-female-zebrafish
#6
Guangfu Liu, Mingjing Ke, Xiaoji Fan, Meng Zhang, Youchao Zhu, Tao Lu, Liwei Sun, Haifeng Qian
Microcystis aeruginosa, a primary species in cyanobacterial blooms, is ubiquitously distributed in water. Microcystins (MCs) purified from M. aeruginosa can exert reproductive toxicity in fish. However, the effects of M. aeruginosa at environmentally relevant levels on the reproductive and endocrine systems of zebrafish are still unknown. The present study investigated the reproductive and endocrine-disrupting toxicity of M. aeruginosa on female zebrafish (Danio rerio) by short-term exposure (96 h). After exposure, marked histological lesions in the liver or gonads, such as nuclear pyknosis and deformation, were observed, and the fertilization rate and hatchability of eggs spawned from treated females were both significantly lower than they were in females in the control group, suggesting the possibility of transgenerational effects of M...
November 1, 2017: Chemosphere
https://www.readbyqxmd.com/read/29106056/microenvironmental-stiffness-of-3d-polymeric-structures-to-study-invasive-rates-of-cancer-cells
#7
Enrico Domenico Lemma, Barbara Spagnolo, Francesco Rizzi, Stefania Corvaglia, Marco Pisanello, Massimo De Vittorio, Ferruccio Pisanello
Cells are highly dynamic elements, continuously interacting with the extracellular environment. Mechanical forces sensed and applied by cells are responsible for cellular adhesion, motility, and deformation, and are heavily involved in determining cancer spreading and metastasis formation. Cell/extracellular matrix interactions are commonly analyzed with the use of hydrogels and 3D microfabricated scaffolds. However, currently available techniques have a limited control over the stiffness of microscaffolds and do not allow for separating environmental properties from biological processes in driving cell mechanical behavior, including nuclear deformability and cell invasiveness...
November 6, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29094303/high-thickness-histological-sections-as-alternative-to-study-the-three-dimensional-microscopic-human-sub-cortical-neuroanatomy
#8
Eduardo Joaquim Lopes Alho, Ana Tereza Di Lorenzo Alho, Lea Grinberg, Edson Amaro, Gláucia Aparecida Bento Dos Santos, Rafael Emídio da Silva, Ricardo Caires Neves, Maryana Alegro, Daniel Boari Coelho, Manoel Jacobsen Teixeira, Erich Talamoni Fonoff, Helmut Heinsen
Stereotaxy is based on the precise image-guided spatial localization of targets within the human brain. Even with the recent advances in MRI technology, histological examination renders different (and complementary) information of the nervous tissue. Although several maps have been selected as a basis for correlating imaging results with the anatomical locations of sub-cortical structures, technical limitations interfere in a point-to-point correlation between imaging and anatomy due to the lack of precise correction for post-mortem tissue deformations caused by tissue fixation and processing...
November 1, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28962187/the-therapeutic-effect-of-zerumbone-on-chronic-gastritis-via-antioxidant-mechanisms
#9
Liqing Li, Liang Kong, Hongquan Song
Effects of zerumbone on chronic gastritis remain unclear. The purpose of this study was to investigate the mechanism of the protective effect of zerumbone on the treatment of chronic gastritis in rats. The animal models of chronic gastritis in rats were established, and the surface damage of gastric mucosa was observed by gross anatomy; the changes of gastric mucosal tissue and surface morphology were observed by pathological sections of gastric mucosal tissues; the expressions of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor 2 (Nrf-2) proteins of gastric mucosal tissues in each group were detected by western blot analysis; the activities of superoxide dismutase (SOD) and catalase (CAT) as well as the contents of reduced glutathione (GSH) and malondialdehyde (MDA) in gastric mucosal tissues were detected by kits...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28919399/a-numerical-model-suggests-the-interplay-between-nuclear-plasticity-and-stiffness-during-a-perfusion-assay
#10
Solenne Deveraux, Rachele Allena, Denis Aubry
Cell deformability is a necessary condition for a cell to be able to migrate, an ability that is vital both for healthy and diseased organisms. The nucleus being the largest and stiffest organelle, it often is a barrier to cell migration. It is thus essential to characterize its mechanical behaviour. First, we numerically investigate the visco-elasto-plastic properties of the isolated nucleus during a compression test. This simulation highlights the impact of the mechanical behaviour of the nuclear lamina and the nucleoplasm on the overall plasticity...
December 21, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28905174/mathematical-development-and-computational-analysis-of-harmonic-phase-magnetic-resonance-imaging-harp-mri-based-on-bloch-nuclear-magnetic-resonance-nmr-diffusion-model-for-myocardial-motion
#11
Michael O Dada, Babatunde Jayeoba, Bamidele O Awojoyogbe, Uno E Uno, Oluseyi E Awe
Harmonic Phase-Magnetic Resonance Imaging (HARP-MRI) is a tagged image analysis method that can measure myocardial motion and strain in near real-time and is considered a potential candidate to make magnetic resonance tagging clinically viable. However, analytical expressions of radially tagged transverse magnetization in polar coordinates (which is required to appropriately describe the shape of the heart) have not been explored because the physics required to directly connect myocardial deformation of tagged Nuclear Magnetic Resonance (NMR) transverse magnetization in polar geometry and the appropriate harmonic phase parameters are not yet available...
September 13, 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/28903618/biomaterial-cues-regulate-epigenetic-state-and-cell-functions-a-systematic-review
#12
Longwei Lv, Yiman Tang, Ping Zhang, Yunsong Liu, Xiangsong Bai, Yongsheng Zhou
Biomaterial cues can act as potent regulators of cell niche and microenvironment. Epigenetic regulation plays an important role in cell functions, including proliferation, differentiation, and reprogramming. It is now well appreciated that biomaterials can alter epigenetic states of cells. In this study, we systematically reviewed the underlying epigenetic mechanisms of how different biomaterial cues, including material chemistry, topography, elasticity, and mechanical stimulus, influence cell functions, such as nuclear deformation, cell proliferation, differentiation, and reprogramming, to summarize the differences and similarities among each biomaterial cues and their mechanisms, and to find common and unique properties of different biomaterial cues...
October 19, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28878082/lysine-242-within-helix-10-of-the-pseudorabies-virus-nuclear-egress-complex-pul31-component-is-critical-for-primary-envelopment-of-nucleocapsids
#13
Sebastian Rönfeldt, Barbara G Klupp, Kati Franzke, Thomas C Mettenleiter
Newly assembled herpesvirus nucleocapsids are translocated from the nucleus to the cytosol by a vesicle-mediated process engaging the nuclear membranes. This transport is governed by the conserved nuclear egress complex (NEC), consisting of the alphaherpesviral pUL34 and pUL31 homologs. The NEC is not only required for efficient nuclear egress but also sufficient for vesicle formation from the inner nuclear membrane (INM) as well as from synthetic lipid bilayers. The recently solved crystal structures for the NECs from different herpesviruses revealed molecular details of this membrane deformation and scission machinery uncovering the interfaces involved in complex and coat formation...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28877489/rupture-dynamics-and-chromatin-herniation-in%C3%A2-deformed-nuclei
#14
Dan Deviri, Dennis E Discher, Sam A Safran
During migration of cells in vivo, in both pathological processes such as cancer metastasis or physiological events such as immune cell migration through tissue, the cells must move through narrow interstitial spaces that can be smaller than the nucleus. This can induce deformation of the nucleus which, according to recent experiments, may result in rupture of the nuclear envelope that can lead to cell death, if not prevented or healed within an appropriate time. The nuclear envelope, which can be modeled as a double lipid bilayer attached to a viscoelastic gel (lamina) whose elasticity and viscosity primarily depend on the lamin composition, may utilize mechanically induced, self-healing mechanisms that allow the hole to be closed after the deformation-induced strains are reduced by leakage of the internal fluid...
September 5, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28867898/cortical-oligodendrocytes-in-kaolin-induced-hydrocephalus-in-wistar-rat-impact-of-degree-and-duration-of-ventriculomegaly
#15
Olugbenga Ayodeji Ayannuga, Thajasvarie Naicker
BACKGROUND: Oligodendrocytes are critical to the function of the brain. They generate the myelin sheath which ensures saltatory conduction, which is a more energy saving and efficient means of axonal impulse transmission. Ventriculomegaly results in neuronal degeneration and astrogliosis. PURPOSE: The effect of the degree of ventriculomegaly on oligodendrocyte in kaolin-induced hydrocephalus and the timeline have not been extensively documented, hence this study...
July 2017: Annals of Neurosciences
https://www.readbyqxmd.com/read/28831291/numerical-simulation-of-a-compound-capsule-in-a-constricted-microchannel
#16
John Gounley, Erik W Draeger, Amanda Randles
Simulations of the passage of eukaryotic cells through a constricted channel aid in studying the properties of cancer cells and their transport in the bloodstream. Compound capsules, which explicitly model the outer cell membrane and nuclear lamina, have the potential to improve computational model fidelity. However, general simulations of compound capsules transiting a constricted microchannel have not been conducted and the influence of the compound capsule model on computational performance is not well known...
2017: Procedia Computer Science
https://www.readbyqxmd.com/read/28819745/bioassessment-of-the-effluents-discharged-from-two-export-oriented-industrial-zones-located-in-kelani-river-basin-sri-lanka-using-erythrocytic-responses-of-the-fish-nile-tilapia-oreochromis-niloticus
#17
C K Hemachandra, A Pathiratne
Complex effluents originating from diverse industrial processes in industrial zones could pose cytotoxic/genotoxic hazards to biota in the receiving ecosystems which cannot be revealed by conventional monitoring methods. This study assessed potential cytotoxicity/genotoxicity of treated effluents of two industrial zones which are discharged into Kelani river, Sri Lanka combining erythrocytic abnormality tests and comet assay of the tropical model fish, Nile tilapia. Exposure of fish to the effluents induced erythrocytic DNA damage and deformed erythrocytes with serrated membranes, vacuolations, nuclear buds and micronuclei showing cytotoxic/genotoxic hazards in all cases...
August 17, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28814533/dietary-selenium-deficiency-or-excess-reduces-sperm-quality-and-testicular-mrna-abundance-of-nuclear-glutathione-peroxidase-4-in-rats
#18
Ji-Chang Zhou, Shijie Zheng, Junluan Mo, Xiongshun Liang, Yuanfei Xu, Huimin Zhang, Chunmei Gong, Xiao-Li Liu, Xin Gen Lei
Background: Glutathione peroxidase (GPX) 4 and selenoprotein P (SELENOP) are abundant, and several variants are expressed in the testis.Objective: We determined the effects of dietary selenium deficiency or excess on sperm quality and expressions of GPX4 and SELENOP variants in rat testis and liver.Methods: After weaning, male Sprague-Dawley rats were fed a Se-deficient basal diet (BD) for 5 wk until they were 9 wk old [mean ± SEM body weight (BW) = 256 ± 5 g]. They were then fed the BD diet alone (deficient) or with 0...
October 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28812999/synthesis-and-evaluation-of-a-sodium-alginate-4-aminosalicylic-acid-based-microporous-hydrogel-for-potential-viscosupplementation-for-joint-injuries-and-arthritis-induced-conditions
#19
Dharmesh R Chejara, Mostafa Mabrouk, Pradeep Kumar, Yahya E Choonara, Pierre P D Kondiah, Ravindra V Badhe, Lisa C du Toit, Divya Bijukumar, Viness Pillay
A microporous hydrogel was developed using sodium alginate (alg) and 4-aminosalicylic acid (4-ASA). The synthesized hydrogel was characterized using various analytical techniques such as Fourier transform infrared spectroscopy (FTIR), Carbon-13 nuclear magnetic resonance ((13)C-NMR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). Additonal carboxyl and hydroxyl functional groups of 4-ASA provided significant lubrication and stress-triggered sol-gel transition to the conjugated hydrogel...
August 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28810706/effects-of-trigonelline-inhibition-of-the-nrf2-transcription-factor-in-vitro-on-echinococcus-granulosus
#20
Wenjuan Qin, Dongfang Guan, Rongji Ma, Rentan Yang, Guoqiang Xing, Hongjuan Shi, Guangyao Tang, Jiajie Li, Hailong Lv, Yufeng Jiang
The aim of this study was to investigate the impact of trigonelline (TRG) on Echinococcus granulosus, and to explore the inhibition impact of nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway on E. granulosus protoscoleces. Echinococcus granulosus protoscoleces were incubated with various concentrations of TRG, and then Nrf2 protein expression and its localization in protoscoleces were detected by western blot analysis and immunofluorescence assay, respectively. Reactive oxygen species (ROS) level in protoscoleces was measured using ROS detection kit...
August 1, 2017: Acta Biochimica et Biophysica Sinica
keyword
keyword
38754
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"