keyword
MENU ▼
Read by QxMD icon Read
search

Nuclear deformation

keyword
https://www.readbyqxmd.com/read/28723250/application-of-molecular-dynamic-simulation-to-study-food-proteins-a-review
#1
Ashutosh Singh, Sai Kranthi Vanga, Valerie Orsat, Vijaya Raghavan
This review presents an overview of the application of molecular dynamic simulation to study food proteins. Processing of food using thermal, chemical, radiation, electromagnetic and mechanical techniques subject its macromolecular bio-components such as carbohydrates and proteins to extreme heat, ionic strength, pH and mechanical deformation. These processing factors affect protein's functional properties such as emulsification, dough formation, gelation, etc. which are associated with changes in their structure...
July 19, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28700212/nuclear-magnetic-resonance-structure-of-an-8-%C3%A3-8-nucleotide-rna-internal-loop-flanked-on-each-side-by-three-watson-crick-pairs-and-comparison-to-three-dimensional-predictions
#2
Andrew D Kauffmann, Scott D Kennedy, Jianbo Zhao, Douglas H Turner
The prediction of RNA three-dimensional structure from sequence alone has been a long-standing goal. High-resolution, experimentally determined structures of simple noncanonical pairings and motifs are critical to the development of prediction programs. Here, we present the nuclear magnetic resonance structure of the (5'CCAGAAACGGAUGGA)2 duplex, which contains an 8 × 8 nucleotide internal loop flanked by three Watson-Crick pairs on each side. The loop is comprised of a central 5'AC/3'CA nearest neighbor flanked by two 3RRs motifs, a known stable motif consisting of three consecutive sheared GA pairs...
July 12, 2017: Biochemistry
https://www.readbyqxmd.com/read/28682632/linking-the-character-of-metal-ligand-bond-to-the-ligand-nmr-shielding-in-transition-metal-complexes-nmr-contributions-from-spin-orbit-coupling
#3
Jan Novotny, Jan Vícha, Pankaj Lochan Bora, Michal Repisky, Michal Straka, Stanislav Komorovsky, Radek Marek
Relativistic effects significantly affect various spectroscopic properties of compounds containing heavy elements. Particularly in Nuclear Magnetic Resonance (NMR) spectroscopy, the heavy atoms strongly influence the NMR shielding constants of neighboring light atoms. In this account we analyze paramagnetic contributions to NMR shielding constants and their modulation by relativistic spin-orbit effects in a series of transition-metal complexes of Pt(II), Au(I), Au(III), and Hg(II). We show how the paramagnetic NMR shielding and spin-orbit effects relate to the character of metal-ligand (M‒L) bond...
July 6, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28676938/the-first-report-of-gynandromorphy-in-termites-isoptera-kalotermitidae-neotermes-koshunensis
#4
Yasushi Miyaguni, Tomonari Nozaki, Toshihisa Yashiro
This is the first report of gynandromorphy in Isoptera. An Asian dry-wood termite, Neotermes koshunensis (Shiraki) [Kalotermitidae], possessing both male and female phenotypic characteristics, was found on Okinawa Island, Japan. This deformed individual showed morphological and anatomical hermaphroditism in the abdomen. The right side of the seventh sternite was the female form and contained an ovary, while the left side was the male form and contained a testis. Genotypic analysis revealed that this individual was a genotypic bilateral chimera...
August 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28668640/spectroscopic-studies-of-the-cytochrome-p450-reaction-mechanisms
#5
REVIEW
Piotr J Mak, Ilia G Denisov
The cytochrome P450 monooxygenases (P450s) are thiolate heme proteins that can, often under physiological conditions, catalyze many distinct oxidative transformations on a wide variety of molecules, including relatively simple alkanes or fatty acids, as well as more complex compounds such as steroids and exogenous pollutants. They perform such impressive chemistry utilizing a sophisticated catalytic cycle that involves a series of consecutive chemical transformations of heme prosthetic group. Each of these steps provides a unique spectral signature that reflects changes in oxidation or spin states, deformation of the porphyrin ring or alteration of dioxygen moieties...
June 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28662306/oxidative-stress-and-chronic-inflammation-in-osteoarthritis-can-nrf2-counteract-these-partners-in-crime
#6
REVIEW
Andrey S Marchev, Petya A Dimitrova, Andrew J Burns, Rumen V Kostov, Albena T Dinkova-Kostova, Milen I Georgiev
Osteoarthritis (OA) is an age-related joint degenerative disease associated with pain, joint deformity, and disability. The disease starts with cartilage damage but then progressively involves subchondral bone, causing an imbalance between osteoclast-driven bone resorption and osteoblast-driven remodeling. Here, we summarize the data for the role of oxidative stress and inflammation in OA pathology and discuss how these two processes are integrated during OA progression, as well as their contribution to abnormalities in cartilage/bone metabolism and integrity...
June 29, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28655279/solid-state-deuterium-nmr-study-of-lk%C3%AE-14-peptide-aggregation-in-biosilica
#7
Helen E Ferreira, Gary P Drobny
In nature, organisms including diatoms, radiolaria, and marine sponges use proteins, long chain polyamines, and other organic molecules to regulate the assembly of complex silica-based structures. Here, the authors investigate structural features of small peptides, designed to mimic the silicifying activities of larger proteins found in natural systems. LKα14 (Ac-LKKLLKLLKKLLKL-C), an amphiphilic lysine/leucine repeat peptide with an α-helical secondary structure at polar/apolar interfaces, coprecipitates with silica to form nanospheres...
June 27, 2017: Biointerphases
https://www.readbyqxmd.com/read/28639134/kifc1-and-myosin-va-two-motors-for-acrosomal-biogenesis-and-nuclear-shaping-during-spermiogenesis-of-portunus-trituberculatus
#8
Dan-Dan Ma, Meng-Ying Pan, Cong-Cong Hou, Fu-Qing Tan, Wan-Xi Yang
To investigate the molecular mechanisms underlying the spermiogenesis of the swimming crab Portunus trituberculatus, full lengths of motor proteins KIFC1 and myosin Va were cloned by rapid-amplification of cDNA ends from P. trituberculatus testes cDNA, and their respective probes and specific antibodies were used to track their localization during sperm maturation. Antisense probes were designed from the gene sequences and used to detect the mRNA levels of each gene. According to the results of fluorescence in situ hybridization (FISH), the transcription of kifc1 and myosin Va began at the mid-stage of spermatids, with the kifc1 mRNA being most active at the location where the acrosome cap was formed and the myosin Va was more concentrated in the acrosome complex...
June 21, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28619289/validation-of-a-method-for-dose-of-the-day-calculation-in-head-neck-tomotherapy-by-using-planning-ct-to-mvct-deformable-image-registration
#9
M Branchini, C Fiorino, I Dell'Oca, M L Belli, L Perna, N Di Muzio, R Calandrino, S Broggi
PURPOSE: The aim of this study was to test the feasibility and dosimetric accuracy of a method that employs planning CT-to-MVCT deformable image registration (DIR) for calculation of the daily dose for head and neck (HN) patients treated with Helical Tomotherapy (HT). METHODS: For each patient, the planning kVCT (CTplan) was deformably registered to the MVCT acquired at the 15th therapy session (MV15) with a B-Spline Free Form algorithm using Mattes mutual information (open-source software 3D Slicer), resulting in a deformed CT (CTdef)...
July 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28598677/e2-decay-strength-of-the-m1-scissors-mode-of-156-gd-and-its-first-excited-rotational-state
#10
T Beck, J Beller, N Pietralla, M Bhike, J Birkhan, V Derya, U Gayer, A Hennig, J Isaak, B Löher, V Yu Ponomarev, A Richter, C Romig, D Savran, M Scheck, W Tornow, V Werner, A Zilges, M Zweidinger
The E2/M1 multipole mixing ratio δ_{1→2} of the 1_{sc}^{+}→2_{1}^{+} γ-ray decay in ^{156}Gd and hence the isovector E2 transition rate of the scissors mode of a well-deformed rotational nucleus has been measured for the first time. It has been obtained from the angular distribution of an artificial quasimonochromatic linearly polarized γ-ray beam of energy 3.07(6) MeV scattered inelastically off an isotopically highly enriched ^{156}Gd target. The data yield first direct support for the deformation dependence of effective proton and neutron quadrupole boson charges in the framework of algebraic nuclear models...
May 26, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28598657/reduced-transition-probabilities-for-the-gamma-decay-of-the-7-8%C3%A2-ev-isomer-in-229-th
#11
Nikolay Minkov, Adriana Pálffy
The reduced magnetic dipole and electric quadrupole transition probabilities for the radiative decay of the ^{229}Th 7.8  eV isomer to the ground state are predicted within a detailed nuclear-structure model approach. We show that the presence and decay of this isomer can only be accounted for by the Coriolis mixing emerging from a remarkably fine interplay between the coherent quadrupole-octupole motion of the nuclear core and the single-nucleon motion within a reflection-asymmetric deformed potential...
May 26, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28591600/elastic-fluid-model-for-dna-damage-and-mutation-from-nuclear-fluid-segregation-due-to-cell-migration
#12
Rachel R Bennett, Charlotte R Pfeifer, Jerome Irianto, Yuntao Xia, Dennis E Discher, Andrea J Liu
When cells migrate through constricting pores, they incur DNA damage and develop genomic variation. Experiments show that this damage is not due to DNA breakage from mechanical stress on chromatin in the deformed nucleus. Here we propose a model for a mechanism by which nuclear deformation can lead to DNA damage. We treat the nucleus as an elastic-fluid system with an elastic component (chromatin) and fluid component that can be squeezed out when the nucleus is deformed. We couple the elastic-fluid model to the kinetics of DNA breakage and repair by assuming that the local volume fraction of the elastic component controls the rate of damage per unit volume due to naturally occurring DNA breaks, whereas the volume fraction of the fluid component controls the rate of repair of DNA breaks per unit volume by repair factors, which are soluble in the fluid...
June 6, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28583608/the-effect-of-dietary-grape-pomace-supplementation-on-epididymal-sperm-quality-and-testicular-antioxidant-ability-in-ram-lambs
#13
Junxing Zhao, Yaqian Jin, Min Du, Wenzhong Liu, Youshe Ren, Chunxiang Zhang, Jianxin Zhang
Wine grape pomace (WGP) contains a rich source of polyphenols that can act as powerful antioxidants. The objective of this study was to investigate the effect of dietary WGP supplementation on antioxidative activity and epididymal sperm quality in rams. The rams were raised either under free-range or pen conditions, and the pen-raised rams were fed a WGP-containing diet (0, 5% and 10% of dry matter basis) for 74 days. An increase in the concentrations of reactive oxygen species (ROS, P < 0.05) and malondialdehyde (MDA, P < 0...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28566555/cross-linked-matrix-rigidity-and-soluble-retinoids-synergize-in-nuclear-lamina-regulation-of-stem-cell-differentiation
#14
Irena L Ivanovska, Joe Swift, Kyle Spinler, Dave Dingal, Sangkyun Cho, Dennis E Discher
Synergistic cues from extracellular matrix and soluble factors are often obscure in differentiation. Here, the rigidity of crosslinked collagen synergizes with retinoids in the osteogenesis of human marrow mesenchymal stem cells (MSCs). Collagen nano-films serve as a model matrix that MSCs can easily deform unless the film is enzymatically crosslinked, which promotes the spreading of cells and the stiffening of nuclei as both actomyosin assembly and nucleoskeletal lamin-A increase. Expression of lamin-A is known to be controlled by Retinoic Acid Receptor (RAR) transcription factors, but rigid matrix is needed to induce rapid nuclear accumulation of the RARG isoform and subsequent expression of osteogenic markers...
May 31, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28542912/the-nucleus-is-irreversibly-shaped-by-motion-of-cell-boundaries-in-cancer-and-non-cancer-cells
#15
Vincent J Tocco, Yuan Li, Keith G Christopher, James H Matthews, Varun Aggarwal, Lauren Paschall, Hendrik Luesch, Jonathan D Licht, Richard B Dickinson, Tanmay P Lele
Actomyosin stress fibers impinge on the nucleus and can exert compressive forces on it. These compressive forces have been proposed to elongate nuclei in fibroblasts, and lead to abnormally shaped nuclei in cancer cells. In these models, the elongated or flattened nuclear shape is proposed to store elastic energy. However, we found that deformed shapes of nuclei are unchanged even after removal of the cell with micro-dissection, both for smooth, elongated nuclei in fibroblasts and abnormally shaped nuclei in breast cancer cells...
May 25, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28530826/high-speed-atomic-force-microscopy-reveals-loss-of-nuclear-pore-resilience-as-a-dying-code-in-colorectal-cancer-cells
#16
Mahmoud Shaaban Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Richard W Wong
Nuclear pore complexes (NPCs) are the sole turnstile implanted in the nuclear envelope (NE), acting as a central nanoregulator of transport between the cytosol and the nucleus. NPCs consist of ∼30 proteins, termed nucleoporins. About one-third of nucleoporins harbor natively unstructured, intrinsically disordered phenylalanine-glycine strings (FG-Nups), which engage in transport selectivity. Because the barriers insert deeply in the NPC, they are nearly inaccessible. Several in vitro barrier models have been proposed; however, the dynamic FG-Nups protein molecules themselves are imperceptible in vivo...
June 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28528307/extracellular-atp-is-a-key-modulator-of-alveolar-bone-loss-in-periodontitis
#17
REVIEW
Itzhak Binderman, Nasir Gadban, Avinoam Yaffe
Periodontal diseases are initiated by pathogenic bacterial biofilm activity that induces a host inflammatory cells immune response, degradation of dento gingival fibrous tissue and its detachment from root cementum. It is well accepted, that osteoclastic alveolar bone loss is governed exclusively through secretion of proinflammatory cytokines. Nevertheless, our findings suggest that once degradation of collagen fibers by MMPs occurs, a drop of cellular strains cause immediate release of ATP from marginal gingival fibroblasts, cell deformation and influx of Ca+2...
May 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28514142/nonmonotonic-self-deformation-of-cell-nuclei-on-topological-surfaces-with-micropillar-array
#18
Xiangnan Liu, Ruili Liu, Yexin Gu, Jiandong Ding
Cells respond to the mechanical signals from their surroundings and integrate physiochemical signals to initiate intricate mechanochemical processes. While many studies indicate that topological features of biomaterials impact cellular behaviors profoundly, little research has focused on the nuclear response to a mechanical force generated by a topological surface. Here, we fabricated a polymeric micropillar array with an appropriate dimension to induce a severe self-deformation of cell nuclei and investigated how the nuclear shape changed over time...
June 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28494327/magnetic-field-induced-effects-on-nmr-properties
#19
J Jokisaari, A M Kantola, J Vaara
In principle, all the NMR observables, spin-spin coupling J, nuclear shielding σ and quadrupole coupling q, are magnetic field-dependent. The field dependence may be classified into two categories: direct and indirect (apparent) dependence. The former arises from the magnetic field-induced deformation of the molecular electronic cloud, while the latter stems from a slightly anisotropic orientation distribution of molecules, due to the interaction between the anisotropy of the molecular susceptibility tensor and the external magnetic field...
April 26, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28485113/optomechanical-measurement-of-the-role-of-lamins-in-whole-cell-deformability
#20
Thorsten Kolb, Julia Kraxner, Kai Skodzek, Michael Haug, Dean Crawford, Kendra K Maaß, Katerina E Aifantis, Graeme Whyte
There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension...
May 9, 2017: Journal of Biophotonics
keyword
keyword
38754
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"