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https://www.readbyqxmd.com/read/29679718/identification-of-differential-phosphorylation-and-sub-cellular-localization-of-the-metastasis-suppressor-ndrg1
#1
Kyung Chan Park, Sharleen V Menezes, Danuta S Kalinowski, Sumit Sahni, Patric J Jansson, Zaklina Kovacevic, Des R Richardson
The metastasis suppressor, N-myc downstream regulated gene-1 (NDRG1), exhibits pleiotropic activity, inhibiting metastasis of various tumor-types, while being correlated with metastasis in others. Notably, NDRG1 phosphorylation and cleavage are associated with its function, although it is unclear if these modifications occur universally, or selectively, in different cancer cell-types and if it contributes to its pleiotropy. Considering the suggested DNA repair role of nuclear NDRG1, the effects of the above post-translational modifications on its nuclear localization was examined...
April 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29679646/chronic-methamphetamine-exposure-attenuates-neural-activation-in-hypothalamic-pituitary-adrenal-axis-associated-brain-regions-in-a-sex-specific-manner
#2
Jason S Jacobskind, Zachary J Rosinger, Tiffany Gonzalez, Kristen L Zuloaga, Damian G Zuloaga
Sex differences in methamphetamine (MA) abuse and consequences of MA have been reported with females showing an increased addiction phenotype and withdrawal symptoms. One mechanism through which these effects might occur is via sex-specific alterations in the hypothalamic-pituitary-adrenal (HPA) axis and its associated brain regions. In this study, mice were administered MA (5 mg/kg) or saline for 10 consecutive days. During early withdrawal, anxiety-like behaviors were assessed in the open field, light/dark box, and elevated plus maze...
April 18, 2018: Neuroscience
https://www.readbyqxmd.com/read/29679641/ethanol-induced-cardiomyocyte-toxicity-involves-autophagy-nfkb-transcription-factor
#3
Jessica Maiuolo, Alessia Maretta, Micaela Gliozzi, Vincenzo Musolino, Cristina Carresi, Francesca Bosco, Rocco Mollace, Federica Scarano, Ernesto Palma, Miriam Scicchitano, Saverio Nucera, Domenico Sergi, Saverio Muscoli, Santo Gratteri, Carolina Muscoli, Vincenzo Mollace
Chronic ethanol (EtOH) consumption causes early detrimental consequences in many tissues including the myocardium, though the molecular mechanisms leading to the alcoholic cardiomyopathy (ACM) still remain to be elucidated. Here, we studied several biomolecular changes occurring in cardiomyoblasts after their exposure to sublethal concentrations of EtOH and the potential synergistic effect with methylmercury (MM) or doxorubicin (DOXO), which are known to produce direct myocardial dysfunction. In addition, the possible role of autophagic responses and Nuclear Factor kappa-B (NFkB) modulation in early post-alcoholic myocardial damage has been investigated...
April 18, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29679205/changes-in-the-position-and-volume-of-inactive-x-chromosomes-during-the-g0-g1-transition
#4
Guoliang Lyu, Tan Tan, Yiting Guan, Lei Sun, Qianjin Liang, Wei Tao
In female mammals, each cell silences one X chromosome by converting it into transcriptionally inert heterochromatin. The inactivation is concomitant with epigenetic changes including methylation of specific histone residues and incorporation of macroH2A. Such epigenetic changes may exert influence on the positioning of the inactive X chromosome (Xi) within the nucleus beyond the level of chromatin structure. However, the dynamic positioning of the inactive X chromosome during cell cycle remains unclear. Here, we show that H3K27me3 is a cell-cycle-independent marker for the inactivated X chromosomes in WI38 cells...
April 21, 2018: Chromosome Research
https://www.readbyqxmd.com/read/29679024/cysteine-protease-cathepsins-in-cardiovascular-disease-from-basic-research-to-clinical-trials
#5
REVIEW
Cong-Lin Liu, Junli Guo, Xian Zhang, Galina K Sukhova, Peter Libby, Guo-Ping Shi
Cysteine protease cathepsins have traditionally been considered as lysosome-restricted proteases that mediate proteolysis of unwanted proteins. However, studies from the past decade demonstrate that these proteases are localized not only in acidic compartments (endosomes and lysosomes), where they participate in intracellular protein degradation, but also in the extracellular milieu, plasma membrane, cytosol, nucleus, and nuclear membrane, where they mediate extracellular matrix protein degradation, cell signalling, and protein processing and trafficking through the plasma and nuclear membranes and between intracellular organelles...
April 20, 2018: Nature Reviews. Cardiology
https://www.readbyqxmd.com/read/29678206/biliverdin-reductase-a-attenuated-gmh-induced-inflammatory-response-in-the-spleen-by-inhibiting-toll-like-receptor-4-through-enos-no-pathway
#6
Yiting Zhang, Yan Ding, Tai Lu, Yixin Zhang, Ningbo Xu, Devin W McBride, Jiping Tang, John H Zhang
BACKGROUND: Germinal matrix hemorrhage (GMH) is a common neurologic event with high morbidity and mortality in preterm infants. Spleen has been reported to play a critical role in inflammatory responses by regulating peripheral immune cells which contributes to secondary brain injury. METHODS: The current study investigated the mechanistic role of biliverdin reductase-A (BLVRA) in the splenic response and brain damage in neonates following a collagenase GMH model...
April 20, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29678167/development-of-leafhopper-cell-culture-to-trace-the-early-infection-process-of-a-nucleorhabdovirus-rice-yellow-stunt-virus-in-insect-vector-cells
#7
Haitao Wang, Juan Wang, Yunjie Xie, Zhijun Fu, Taiyun Wei, Xiao-Feng Zhang
BACKGROUND: In China, the rice pathogen Rice yellow stunt virus (RYSV), a member of the genus Nucleorhabdovirus in the family Rhabdoviridae, was a severe threat to rice production during the1960s and1970s. Fundamental aspects of the biology of this virus such as protein localization and formation of the RYSV viroplasm during infection of insect vector cells are largely unexplored. The specific role(s) of the structural proteins nucleoprotein (N) and phosphoprotein (P) in the assembly of the viroplasm during RYSV infection in insect vector is also unclear...
April 20, 2018: Virology Journal
https://www.readbyqxmd.com/read/29677448/self-assembled-ph-responsive-polymeric-micelles-for-highly-efficient-non-cytotoxic-delivery-of-doxorubicin-chemotherapy-to-inhibit-macrophage-activation-in-vitro-investigation
#8
Zhi-Sheng Liao, Shan-You Huang, Jyun-Jie Huang, Jem-Kun Chen, Ai-Wei Lee, Juin-Yih Lai, Duu-Jong Lee, Chih-Chia Cheng
Self-assembled pH-responsive polymeric micelles, a combination of hydrophilic poly(ethylene glycol) segments and hydrogen bonding interactions within a biocompatible polyurethane substrate, can spontaneously self-assemble into highly controlled, nanosized micelles in aqueous solution. These newly-developed micelles exhibit excellent pH-responsive behavior and biocompatibility, highly controlled drug (doxorubicin; DOX) release behavior and high drug encapsulation stability in different aqueous environments, making the micelles highly attractive potential candidates for safer, more effective drug delivery in applications such as cancer chemotherapy...
April 20, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29677202/%C3%AE-1-integrins-mediate-the-bmp2-dependent-transcriptional-control-of-osteoblast-differentiation-and-osteogenesis
#9
Molly Brunner, Noémie Mandier, Thierry Gautier, Genevieve Chevalier, Anne-Sophie Ribba, Philippe Guardiola, Marc R Block, Daniel Bouvard
Osteoblast differentiation is a highly regulated process that requires coordinated information from both soluble factors and the extracellular matrix. Among these extracellular stimuli, chemical and physical properties of the matrix are sensed through cell surface receptors such as integrins and transmitted into the nucleus to drive specific gene expression. Here, we showed that the conditional deletion of β1 integrins in the osteo-precursor population severely impacts bone formation and homeostasis both in vivo and in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29676725/bombyx-mori-nucleopolyhedrovirus-orf40-is-essential-for-budded-virus-production-and-occlusion-derived-virus-envelopment
#10
Yunwang Shen, Min Feng, Xiaofeng Wu
ORF40 (bm40) of the Bombyx mori nucleopolyhedrovirus (BmNPV) encodes a homologue of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) AC51 and is a highly conserved gene in sequenced alphabaculoviruses. To investigate the role of bm40 in the baculovirus infection cycle, a bm40 knockout BmNPV bacmid was constructed via homologous recombination in Escherichia coli. Western blotting analysis revealed that bm40 is a late gene during virus infection. Compared with wild-type and repair viruses, the knockout virus exhibited a single-cell infection phenotype...
April 19, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29676720/mutagenic-repair-of-double-stranded-dna-breaks-in-vaccinia-virus-genomes-requires-cellular-dna-ligase-iv-activity-in-the-cytosol
#11
Rutger David Luteijn, Ingo Drexler, Geoffrey L Smith, Robert Jan Lebbink, Emmanuel J H J Wiertz
Poxviruses comprise a group of large dsDNA viruses that include members relevant to human and animal health, such as variola virus, monkeypox virus, cowpox virus and vaccinia virus (VACV). Poxviruses are remarkable for their unique replication cycle, which is restricted to the cytoplasm of infected cells. The independence from the host nucleus requires poxviruses to encode most of the enzymes involved in DNA replication, transcription and processing. Here, we use the CRISPR/Cas9 genome engineering system to induce DNA damage to VACV (strain Western Reserve) genomes...
April 20, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29675925/nrf2-and-keap1-abnormalities-in-esophageal-squamous-cell-carcinoma-and-association-with-the-effect-of-chemoradiotherapy
#12
Jingze Zhang, Qinghua Jiao, Li Kong, Jing Yu, Aiju Fang, Minghuan Li, Jinming Yu
BACKGROUND: The Keap1-Nrf2 pathway is a key antioxidant and redox signaling cascade. Pathway abnormalities enhance the reactive oxygen species scavenging ability of cancer cells; thus the pathway is involved in carcinogenesis and resistance to chemoradiotherapy (CRT). This retrospective study was conducted to examine the status of the Keap1-Nrf2 pathway in locally advanced esophageal squamous cell carcinoma (ESCC) and to analyze its prognostic value in patients receiving CRT. METHODS: Nrf2 and Keap1 expression were immunohistochemically examined in 152 ESCC and 31 normal esophageal mucosae...
April 19, 2018: Thoracic Cancer
https://www.readbyqxmd.com/read/29675232/diselenolane-mediated-cellular-uptake
#13
Nicolas Chuard, Amalia I Poblador-Bahamonde, Lili Zong, Eline Bartolami, Jana Hildebrandt, Wolfgang Weigand, Naomi Sakai, Stefan Matile
The emerging power of thiol-mediated uptake with strained disulfides called for a move from sulfur to selenium. We report that according to results with fluorescent model substrates, cellular uptake with 1,2-diselenolanes exceeds uptake with 1,2-dithiolanes and epidithiodiketopiperazines with regard to efficiency as well as intracellular localization. The diselenide analog of lipoic acid performs best. This 1,2-diselenolane delivers fluorophores efficiently to the cytosol of HeLa Kyoto cells, without detectable endosomal capture as with 1,2-dithiolanes or dominant escape into the nucleus as with epidithiodiketopiperazines...
February 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29674236/sub-lethal-ultraviolet-b-irradiation-and-poly-i-c-treatment-synergistically-induced-apoptosis-of-hacat-cells-through-nf-%C3%AE%C2%BAb-pathway
#14
Wuxiyar Otkur, Weiwei Liu, Jinda Wang, Xingfan Jia, Dianchao Huang, Fang Wang, Toshihiko Hayashi, Shin-Ichi Tashiro, Satoshi Onodera, Takashi Ikejima
Ultraviolet B (UVB) irradiation exerts multiple effects on skin cells, inducing apoptosis, senescence and carcinogenesis. Toll-like receptor 3, a member of pattern recognition receptors, is reported to initiate inflammation by recognizing double-strand RNA (dsRNA) released from UVB-irradiated cells. It has not been studied, however, whether apoptosis induction in UVB irradiation is attributed to TLR3 activation. Here, we report on the pro-apoptotic role of TLR3 in UVB-irradiated epidermal cells. Poly I:C, an analogue of dsRNA that activates TLR3, was used in combination with sub-lethal UVB (4...
April 16, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29674123/effects-of-dietary-toxic-cyanobacteria-and-ammonia-exposure-on-immune-function-of-blunt-snout-bream-megalabrama-amblycephala
#15
Hu Xia, Ting Song, Li Wang, Liangsen Jiang, Qiting Zhou, Weimin Wang, Liangguo Liu, Pinhong Yang, Xuezhen Zhang
Cyanobacterial blooms caused by water eutrophication have become a worldwide problem. During the degradation of toxic cyanobacterial blooms, elevated ammonia and microcystins concentrations co-occur and exert toxicity on fish. Up to now, the combined effect of microcystins and ammonia on fish immunotoxicity has not been reported. The present study investigated immune responses of blunt snout bream (Megalabrama amblycephala) to dietary toxic cyanobacteria and ammonia exposure. Megalobrama amblycephala were exposed to solutions with different concentrations of NH3 -N (0, 0...
April 16, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29674090/oscillatory-local-field-potentials-of-the-nucleus-accumbens-and-the-anterior-limb-of-the-internal-capsule-in-heroin-addicts
#16
Shunnan Ge, Xinyi Geng, Xuelian Wang, Nan Li, Lei Chen, Xinjing Zhang, Yongzhi Huang, Yang Li, Yukun Chen, Shouyan Wang, Guodong Gao
OBJECTIVES: The nucleus accumbens (NAc) is known to regulate the motivation and underlie addictive behaviors, and the anterior limb of the internal capsule (ALIC) is involved in several psychiatric disorders. Our study aimed to explore the functions of NAc and ALIC electrophysiologically. METHODS: The local field potentials (LFPs) of the NAc and ALIC were recorded from 7 heroin addicts treated with deep brain stimulation. Correlation analysis was made between LFP powers in various frequency bands and the subjects' neuropsychological test scores; coherence was calculated for the LFPs in NAc and ALIC...
March 30, 2018: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/29673902/la-ssb-chimeric-autoantibody-receptor-modified-nk92mi-cells-for-targeted-therapy-of-autoimmune-disease
#17
Huimin Meng, Xue Sun, Yanhui Song, Jianxuan Zou, Gangli An, Zeming Jin, Lin Yang
It has been long sought to specifically eliminate B-cell clones that generate autoreactive antibodies, while sparing the immune system when combating autoimmune disease. Although it was impossible to achieve this goal before, newly developed techniques have made it feasible today. Autoantibodies against La/SSB were involved in several autoimmune diseases. Here, we aimed to introduce La/SSB epitope-based chimeric autoantibody receptors (CAAR) into NK92MI cells enabled it to destroy the corresponding La/SSB-specific B cell receptor (BCR) -bearing lymphoma cells (LaA-BCR-Romas, LaA-BCR-Maver-1, and LaA-BCR-Jurkat cells)...
April 16, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29673663/hmgb1-rage-signaling-pathway-in-pprom
#18
Huan Yan, Linlin Zhu, Zhan Zhang, Hong Li, Pengyun Li, Yan Wang, Maodong Leng
OBJECTIVE: Increased inflammation of the placenta is considered as a risk factor and a promoter of preterm premature rupture of the membranes (pPROM). High-mobility group box 1 (HMGB1) is a recently identified inflammatory cytokine, and HMGB1-RAGE signaling pathway has been associated with many pathophysiological processes. This study aims to reveal the mechanisms of HMGB1-RAGE signaling pathway in pPROM. MATERIALS AND METHODS: The mRNA levels of relative gene of HMGB1 pathway, HMGB1, RAGE, NF-κBp65, MMP-9 and MMP-2, were analyzed by real-time PCR in placentas collected from 60 normal term women, 60 women with PROM and 60 women with pPROM...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29673646/polymeric-micelles-encapsulating-ph-responsive-doxorubicin-prodrug-and-glutathione-activated-zinc-ii-phthalocyanine-for-combined-chemotherapy-and-photodynamic-therapy
#19
Di Gao, Pui-Chi Lo
A series of polymeric micelles encapsulating different ratios of doxorubicin (DOX) and zinc(II) phthalocyanine (ZnPc) have been prepared for dual chemotherapy and photodynamic therapy (PDT). The amphiphilic block copolymers consist of methoxypolyethylene glycol (PEG) and poly(β-benzyl-l-aspartate) (PBLA), in which DOX and ZnPc were conjugated to the aspartate side chain through an acid-labile hydrazone linker and a redox-responsive disulfide linker, respectively. The polymers were self-assembled into spherical polymeric micelles with diameters of about 160-180 nm and their surface charges were found to be nearly neutral due to the outermost PEG layer...
April 16, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29673631/comparison-between-the-prechopping-method-with-a-reverse-chopper-and-the-routine-stop-and-chop-method-in-treating-cataract-with-grade-iv-hard-nucleus
#20
Y Zhao, K Yang, J Li, S Zhu
The aim of this study was to compare the safety and efficacy between the prechopping method using a Reverse Chopper (iHPC) and the routine stop-and-chop (RSC) method in phacoemulsification of cataracts with grade IV hard nuclei (HNC (≥IV)). A total of 32 patients (32 eyes) scheduled for Phaco combined with intraocular lens (Phaco-IOL) for HNC (≥IV) from March 2015 to January 2016 were enrolled and randomly divided into two groups (the iHPC group, n=16 and the RSC group, n=16) so as to compare the effective intraoperative Phaco time, corneal edema 1-3 days after surgery, best corrected visual acuity (BCVA), and rate of loss of corneal endothelial cells (CECs) 1 month after surgery...
April 17, 2018: Journal Français D'ophtalmologie
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