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Streptococcus agalactiae

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https://www.readbyqxmd.com/read/29659565/cyclic-di-amp-regulation-of-osmotic-homeostasis-is-essential-in-group-b-streptococcus
#1
Laura Devaux, Dona Sleiman, Maria-Vittoria Mazzuoli, Myriam Gominet, Philippe Lanotte, Patrick Trieu-Cuot, Pierre-Alexandre Kaminski, Arnaud Firon
Cyclic nucleotides are universally used as secondary messengers to control cellular physiology. Among these signalling molecules, cyclic di-adenosine monophosphate (c-di-AMP) is a specific bacterial second messenger recognized by host cells during infections and its synthesis is assumed to be necessary for bacterial growth by controlling a conserved and essential cellular function. In this study, we sought to identify the main c-di-AMP dependent pathway in Streptococcus agalactiae, the etiological agent of neonatal septicaemia and meningitis...
April 16, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29658084/modeling-of-3d-structure-of-chimeric-constructs-based-on-hemagglutinin-of-influenza-virus-and-immunogenic-epitopes-of-streptococcus-agalactiae
#2
E A Fedorova, T A Smolonogina, I N Isakova-Sivak, D A Koren'kov, T S Kotomina, G F Leont'eva, A N Suvorov, L G Rudenko
A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed. Based on the results of simulation, the most promising variants were selected; they represented fragments of lipoprotein ScaAB lacking N-terminal domain bound to hemagglutinin via a flexible linker...
April 16, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29651820/seroprevalence-of-opsonophagocytic-antibodies-against-serotype-ia-ib-ii-iii-and-v-group-b-streptococcus-among-korean-population
#3
Ji Hyen Lee, Han Wool Kim, Kyung Hyo Kim
BACKGROUND: Invasive Streptococcus agalactiae (group B streptococcus, GBS) infection most commonly occurs in infants; however, cases of GBS infection in adults, particularly in the elderly with significant underlying diseases, are being increasingly reported. We analyzed the serotype specific opsonophagocytic antibodies (the major mechanism of protection against GBS) in infants, adults, and the elderly. METHODS: The opsonization indices (OIs) of antibodies against serotype Ia, Ib, II, III, and V GBS were studied in 89 infants, 35 adults (age, 30-50 years), and 62 elderly individuals (age, 65-85 years) according to the University of Alabama at Birmingham GBS opsonophagocytic killing assay protocol (www...
April 16, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29643850/a-perspective-on-the-potential-zoonotic-role-of-streptococcus-agalactiae-searching-for-a-missing-link-in-alternative-transmission-routes
#4
Ana C N Botelho, Ana F M Ferreira, Sergio E L Fracalanzza, Lucia M Teixeira, Tatiana C A Pinto
No abstract text is available yet for this article.
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29608253/the-direct-and-indirect-association-of-cervical-microbiota-with-the-risk-of-cervical-intraepithelial-neoplasia
#5
Chaoting Zhang, Ying Liu, Weijiao Gao, Yaqi Pan, Yunong Gao, Jing Shen, Hongchao Xiong
Cervical microbiota composition is associated with cervical HPV infection and CIN severity. Previous studies only assessed the total association between cervical microbiota and HPV infections or CINs, and yet no study reported the direct and indirect associations between cervical microbiota and CINs mediated by HPV infection, respectively. The aim of this study was to investigate the direct and indirect associations between microbiotas and CIN severity. Cervical microbiota of 126 women with CIN 1- (normal cytology and CIN 1) and 40 with CIN 2+ (CIN 2 and CIN 3) were analyzed using Illumina sequencing based on the 16S rRNA gene...
April 2, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29606774/assessing-the-fatty-acid-essential-oil-composition-their-radical-scavenging-and-antibacterial-activities-of-schinus-terebinthifolius-raddi-leaves-and-twigs
#6
Asma Ennigrou, Hervé Casabianca, Emmanuelle Vulliet, Belgacem Hanchi, Karim Hosni
The fatty acid, essential oil compositions and their respective antioxidant and antibacterial activities was determined in Schinus terebinthifolius Raddi leaves and twigs. The lipid content ranged from 1.75 to 4.65% in twigs and leaves, respectively. Thirteen fatty acids were identified with α-linolenic (C18:3), palmitic (C16:0) and linoleic (C18:2) acids being the main components. The essential oils of both organs were characterized by a high amount of monoterpene hydrocarbons (68.91-74.88%) with α-phellandrene (33...
April 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29600772/antimicrobial-resistance-in-streptococcus-spp
#7
Marisa Haenni, Agnese Lupo, Jean-Yves Madec
The genus Streptococcus includes Gram-positive organisms shaped in cocci and organized in chains. They are commensals, pathogens, and opportunistic pathogens for humans and animals. Most Streptococcus species of veterinary relevance have a specific ecological niche, such as S. uberis , which is almost exclusively an environmental pathogen causing bovine mastitis. In contrast, S. suis can be considered as a true zoonotic pathogen, causing specific diseases in humans after contact with infected animals or derived food products...
March 2018: Microbiology Spectrum
https://www.readbyqxmd.com/read/29593684/group-b-streptococcal-colonization-molecular-characteristics-and-epidemiology
#8
REVIEW
Sarah Shabayek, Barbara Spellerberg
Streptococcus agalactiae or group B streptococcus (GBS) is a leading cause of serious neonatal infections. GBS is an opportunistic commensal constituting a part of the intestinal and vaginal physiologic flora and maternal colonization is the principal route of GBS transmission. GBS is a pathobiont that converts from the asymptomatic mucosal carriage state to a major bacterial pathogen causing severe invasive infections. At present, as many as 10 serotypes (Ia, Ib, and II-IX) are recognized. The aim of the current review is to shed new light on the latest epidemiological data and clonal distribution of GBS in addition to discussing the most important colonization determinants at a molecular level...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29581074/susceptibility-profiles-of-bacteria-causing-urinary-tract-infection-in-mexico-single-center-experience-with-10-years-results
#9
Francisco Alejandro Lagunas-Rangel
OBJECTIVES: The aim of this retrospective study was to describe urinary tract infections (UTIs) diagnosed in a single center in Mexico through 10 years of results, with a particular emphasis on the etiology, patterns of antimicrobial susceptibility and incidence of extended-spectrum β-lactamase-producing strains (ESBL). METHODS: This study involved all urine samples analyzed during the period between March 2007 and September 2017. Isolated uropathogens were identified to species level using routine laboratory methods Antimicrobial susceptibility was determined by the disc diffusion method...
March 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29567126/distribution-of-streptococcal-groups-causing-infective-endocarditis-a-descriptive-study
#10
So Lim Kim, Steven M Gordon, Nabin K Shrestha
The purpose of this study was to describe the distribution of streptococci causing infective endocarditis (IE). A total of 296 patients with definite IE admitted from July 2007 to December 2014 were identified, with microbial identification done by a combination of blood culture, valve culture, and valve polymerase chain reaction (PCR). The overall distribution of streptococci was 76% viridans (n = 224), 17% pyogenic (50), 6% nutritionally variant (17), and 2% anaerobic (5). Sixty-three (21%) viridans group streptococci were not identified further...
February 24, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29550602/effects-of-dietary-lactobacillus-rhamnosus-jcm1136-and-lactococcus-lactis-subsp-lactis-jcm5805-on-the-growth-intestinal-microbiota-morphology-immune-response-and-disease-resistance-of-juvenile-nile-tilapia-oreochromis-niloticus
#11
Yun Xia, Maixin Lu, Gang Chen, Jianmeng Cao, Fengying Gao, Miao Wang, Zhigang Liu, Defeng Zhang, Huaping Zhu, Mengmeng Yi
The present study aimed to evaluate the individual and combined effects of Lactobacillus rhamnosus (LR) JCM1136 and Lactococcus lactis subsp. lactis (LL) JCM5805 on the growth, intestinal microbiota, intestinal morphology, immune response and disease resistance of juvenile Nile tilapia (Oreochromis niloticus). A total of 720 apparently healthy juvenile Nile tilapia (0.20 ± 0.05 g) were randomly divided into four equal groups. Fish were fed with a basal diet (CK) supplemented with JCM1136 (LR), JCM5805 (LL), and JCM1136 + JCM5805 (LR+LL) at 1 × 108  CFU/g basal diet for 6 weeks, followed by a basal diet for 1 week...
March 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29550124/the-production-characteristics-of-volatile-organic-compounds-and-their-relation-to-growth-status-of-staphylococcus-aureus-in-milk-environment
#12
J Chen, J N Tang, K L Hu, Y Y Zhao, C Tang
In our previous research, 3-methyl-butanal and 3-methyl-butanoic acid were identified as representative and specific volatile organic compounds released by Staphylococcus aureus in broth. In this study, we explored the production of the 2 volatiles and their correlation to Staph. aureus growth in milk under different conditions. We found significant correlations between the production of 3-methyl-butanoic acid and cell counts of 5 Staph. aureus strains in sterile milk, and there were no obvious differences for its production among 5 tested strains...
March 14, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29534714/lumbar-spine-epidural-abscess-and-facet-joint-septic-arthritis-due-to-streptococcus-agalactiae-a-case-report
#13
Costansia Bureta, Hiroyuki Tominaga, Takuya Yamamoto, Takao Setoguchi, Hideki Kawamura, Satoshi Nagano, Ichiro Kawamura, Masahiko Abematsu, Hironori Kakoi, Yasuhiro Ishidou, Setsuro Komiya
BACKGROUND: Here we report a rare case of lumbar spine epidural abscess and facet joint septic arthritis caused by Streptococcus agalactiae, which had spread to the iliopsoas muscles, leading to urine retention. CASE PRESENTATION: A 68-year-old woman with low back pain experienced a sudden onset of bilateral lower limb weakness, it was followed 14 days later by urine retention. At consultation, magnetic resonance imaging and identification of serum β-hemolytic streptococci provided a diagnosis of Streptococcus agalactiae infection...
March 13, 2018: BMC Surgery
https://www.readbyqxmd.com/read/29527193/-streptococcus-agalactiae-inhibits-candida-albicans-hyphal-development-and-diminishes-host-vaginal-mucosal-th17-response
#14
Xiao-Yu Yu, Fei Fu, Wen-Na Kong, Qian-Kun Xuan, Dong-Hua Wen, Xiao-Qing Chen, Yong-Ming He, Li-Hua He, Jian Guo, Ai-Ping Zhou, Yang-Hong Xi, Li-Jun Ni, Yu-Feng Yao, Wen-Juan Wu
Streptococcus agalactiae and Candida albicans often co-colonize the female genital tract, and under certain conditions induce mucosal inflammation. The role of the interaction between the two organisms in candidal vaginitis is not known. In this study, we found that co-infection with S. agalactiae significantly attenuated the hyphal development of C. albicans , and that EFG1 -Hwp1 signal pathway of C. albicans was involved in this process. In a mouse model of vulvovaginal candidiasis (VVC), the fungal burden and the levels of pro-inflammatory cytokines, IL-1β, IL-6 and TNF-α showed a increase on co-infection with S...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29520519/a-cytosol-derived-factor-of-group-b-streptococcus-prevent-its-invasion-into-human-epithelial-cells
#15
Manju Ohri, Smriti Parashar, Venkatesh S Pai, Sujata Ghosh, Anuradha Chakraborti
Group B streptococcus (GBS) or Streptococcus agalactiae, is an opportunistic pathogen causing a wide range of infections like pneumonia, sepsis, and meningitis in newborn, pregnant women and adults. While this bacterium has adapted well to asymptomatic colonization of adult humans, it still remains a potentially devastating pathogen to susceptible infants. Advances in molecular techniques and refinement of in vitro and in vivo model systems have elucidated key elements of the pathogenic process, from initial attachment to the maternal vaginal epithelium to penetration of the newborn blood-brain barrier...
March 8, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29519594/molecular-characteristics-of-streptococcus-agalactiae-in-a-mother-baby-prospective-cohort-study-implication-for-vaccine-development-and-insights-into-vertical-transmission
#16
Shunming Li, Guoming Wen, Xuelian Cao, Dan Guo, Zhenjiang Yao, Chuan'an Wu, Xiaohua Ye
BACKGROUND: Streptococcus agalactiae (GBS) is a leading cause of neonatal sepsis and meningitis in many countries. This study aimed to determine the molecular characteristics of GBS colonized in mothers and their infants so as to provide implication for vaccine strategies and confirm vertical transmission. METHODS: A prospective cohort study was conducted to recruit 1815 mother-neonate pairs. All GBS isolates from pregnant women and her infants were tested for serotypes, multilocus sequence types and virulence genes...
March 5, 2018: Vaccine
https://www.readbyqxmd.com/read/29518409/abiei-binds-cooperatively-to-the-type-iv-abie-toxin-antitoxin-operator-via-a-positively-charged-surface-and-causes-dna-bending-and-negative-autoregulation
#17
Hannah G Hampton, Simon A Jackson, Robert D Fagerlund, Anne I M Vogel, Ron L Dy, Tim R Blower, Peter C Fineran
Bacteria resist phage infection using multiple strategies, including CRISPR-Cas and abortive infection (Abi) systems. Abi systems provide population-level protection from phage predation, via "altruistic" cell suicide. It has recently been shown that some Abi systems function via a toxin-antitoxin (TA) mechanism, such as the widespread AbiE family. The Streptococcus agalactiae AbiE system consists of a bicistronic operon encoding the AbiEi antitoxin and AbiEii toxin, which function as a Type IV TA system...
March 5, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29515550/comparison-of-meconium-dna-extraction-methods-for-use-in-microbiome-studies
#18
Lisa F Stinson, Jeffrey A Keelan, Matthew S Payne
The establishment of human gut microbiota commences initially in utero . Meconium-the first fecal material passed after birth-can be used to study fetal gut contents; however, processing meconium samples for microbiome studies presents significant technical challenges. Meconium hosts a low biomass microbiome, is tar-like in texture and contains high concentrations of PCR inhibitors. This study aimed to evaluate four different DNA extraction methods to elucidate the most effective method for bacterial DNA recovery and sequencing analysis from first-pass meconium...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29501881/validation-of-igy-for-the-diagnosis-of-streptococcus-agalactiae-caused-endocarditis-and-bacterial-meningitis-in-nile-tilapia-oreochromis-niloticus
#19
Silas F Eto, Dayanne C Fernandes, Alessandra C Moraes, Ed Johnny R Prado, Amanda C Baldassi, Wilson G Manrique, Ives C Silva, Andrea S R Medeiros, Marco A A Belo, Tiago S Balbuena, Samir I Samara, João M Pizauro
Streptococcus agalactiae (Sta), which belongs to Lancefield group B, causes sepsis, endocarditis and bacterial meningitis in human neonates and Nile tilapia. Because the pathophysiology of Sta infection is partially similar in both species, the identification of biomarkers for the diagnosis and study of this disease is of importance for human and animal health. Therefore, in the present study, we produced an immunoglobulin Y (IgY) by immunizing laying hens with Sta proteins and evaluated its ability to detect Sta in paraffinized tilapia brain and cardiac tissue by direct immunofluorescence (IMF) and indirect immunohistochemistry (IHC)...
March 1, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29481905/a-c-type-lectin-cl11x1-like-from-nile-tilapia-oreochromis-niloticus-is-involved-in-host-defense-against-bacterial-infection
#20
Liangliang Mu, Xiaoxue Yin, Yanhui Xiao, Xia Bian, Yanjian Yang, Liting Wu, Jianmin Ye
Collectins, a subfamily of the C-type lectins, are able to bind non-self glycoconjugates on the surface of microorganisms and inhibit infection by direct neutralization, agglutination and/or opsonization, which play important roles in innate immunity. In this study, a CL11X1-like collectin (OnCL11X1) was identified from Nile tilapia (Oreochromis niloticus) and characterized at expression and agglutination functional levels. The open reading frame of OnCL11X1 is 840 bp of nucleotide sequence encoding polypeptides of 279 amino acids...
February 24, 2018: Developmental and Comparative Immunology
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