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Streptococcus agalactiae

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https://www.readbyqxmd.com/read/28705964/draft-genome-sequences-of-seven-streptococcus-agalactiae-strains-isolated-from-camelus-dromedarius-at-the-horn-of-africa
#1
Julian Rothen, Tobias Schindler, Joël F Pothier, Mario Younan, Ulrich Certa, Claudia Daubenberger, Valentin Pflüger, Joerg Jores
We present draft whole-genome sequences of seven Streptococcus agalactiae strains isolated from Camelus dromedarius in Kenya and Somalia. These data are an extension to the group B Streptococcus (GBS) pangenome and might provide more insight into the underlying mechanisms of pathogenicity and antibiotic resistance of camel GBS.
July 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28699699/involvement-of-cholinergic-and-adenosinergic-systems-on-the-branchial-immune-response-of-experimentally-infected-silver-catfish-with-streptococcus-agalactiae
#2
M D Baldissera, C F Souza, P H Doleski, K L S Moreira, M L da Veiga, M I U M da Rocha, R C V Santos, B Baldisserotto
It has been recognized that the cholinergic and adenosinergic systems have an essential role in immune and inflammatory responses during bacterial fish pathogens, such as the enzymes acetylcholinesterase (AChE) and adenosine deaminase (ADA), which are responsible for catalysis of the anti-inflammatory molecules acetylcholine (ACh) and adenosine (Ado) respectively. Thus, the aim of this study was to investigate the involvement of the cholinergic and adenosinergic systems on the immune response and inflammatory process in gills of experimentally infected Rhamdia quelen with Streptococcus agalactiae...
July 12, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28698421/-tricuspid-valve-repair-in-a-patient-with-isolated-tricuspid-valve-infective-endocarditis-with-pulmonary-embolism-report-of-a-case
#3
Hiroyuki Hara, Kenji Minakata, Kazuhisa Sakamoto, Yuki Kuroda, Toshio Harada, Taro Nakatsu, Tomohiro Nakata, Kyokun Uehara, Kazuhiro Yamasaki, Tadashi Ikeda
We report a case of 39-year-old man who developed tricuspid valve infective endocarditis with a complication of pulmonary embolism. He was transferred to our institution because of intermittent fever and enlargement of the vegetation of the tricuspid valve in spite of optimal antibiotics treatment. Computed tomography revealed pulmonary embolism, and transesophageal echocardiography showed a large and mobile vegetation (22×10 mm) on the tricuspid valve with moderate regurgitation. In addition, Streptococcus agalactiae was identified in blood cultures...
July 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28689775/gene-characteristics-immune-and-stress-responses-of-pmprx1-in-black-tiger-shrimp-penaeus-monodon-insights-from-exposure-to-pathogenic-bacteria-and-toxic-environmental-stressors
#4
Ruiqian Bu, Pengfei Wang, Chao Zhao, Weiyang Bao, Lihua Qiu
Peroxiredoxins (Prxs) are ubiquitous, multifunctional and evolutionarily conserved enzymes that can protect cells from oxidative damage caused by ROS and play a vital role in immune responses. Here, a full-length Prx1 cDNA sequence (PmPrx1) was isolated from Penaeus monodon. The PmPrx1 cDNA was 951 base pairs (bp), encoding 198 amino acid polypeptides. The results of qRT-PCR showed that the PmPrx1 mRNA was ubiquitously expressed in all tissues tested and had a comparatively high expression level in immune-associated tissues (gill, hepatopancreas)...
July 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28673237/contribution-of-pilus-type-2b-to-invasive-disease-caused-by-a-streptococcus-agalactiae-st-17-strain
#5
Maddalena Lazzarin, Rong Mu, Monica Fabbrini, Claudia Ghezzo, C Daniela Rinaudo, Kelly S Doran, Immaculada Margarit
BACKGROUND: Group B Streptococcus (GBS) is a major cause of invasive disease especially in neonates. In GBS three structurally distinct pilus polymers have been identified as important virulence factors and promising vaccine candidates. The vast majority of Group B Streptococci belonging to the hypervirulent serotype III ST-17 lineage bear pilus types 1 and 2b. The purpose of this study was to investigate the relative contribution of these two pilus types to the pathogenesis of a ST-17 strain...
July 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28669679/a-novel-one-step-real-time-multiplex-pcr-assay-to-detect-streptococcus-agalactiae-presence-and-serotypes-ia-ib-and-iii
#6
Lucy L Furfaro, Barbara J Chang, Matthew S Payne
Streptococcus agalactiae is the leading cause of early-onset neonatal sepsis. Culture-based screening methods lack the sensitivity of molecular assays and do not indicate serotype; a potentially important virulence marker. We aimed to develop a multiplex PCR to detect S. agalactiae while simultaneously identifying serotypes Ia, Ib, and III; commonly associated with infant disease. Primers were designed to target S. agalactiae serotype-specific cps genes and the dltS gene. The assay was validated with 512 vaginal specimens from pregnant women...
June 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28669650/next-generation-dna-sequencing-of-tissues-from-infected-diabetic-foot-ulcers
#7
M Malone, K Johani, S O Jensen, I B Gosbell, H G Dickson, H Hu, K Vickery
We used next generation DNA sequencing to profile the microbiome of infected Diabetic Foot Ulcers (DFUs). The microbiota was correlated to clinical parameters and treatment outcomes to determine if directed antimicrobial therapy based on conventional microbiological cultures are relevant based on genomic analysis. Patients≥18years presenting with a new Diabetic Foot Infection (DFI) who had not received topical or oral antimicrobials in the two weeks prior to presentation, were eligible for enrolment. Tissue punch biopsies were obtained from infected DFUs for analysis...
June 27, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28669389/cathelicidin-production-and-release-by-mammary-epithelial-cells-during-infectious-mastitis
#8
Tiziana Cubeddu, Carla Cacciotto, Salvatore Pisanu, Vittorio Tedde, Alberto Alberti, Marco Pittau, Simone Dore, Agnese Cannas, Sergio Uzzau, Stefano Rocca, Maria Filippa Addis
Cathelicidins are well-characterized antimicrobial peptides (AMPs) that are present in significant amounts in mastitic milk. Neutrophils are believed to be the main producers of these AMPs, while the role of mammary epithelial cells (MECs) in their production and release is still unclear. In this work, cathelicidin production patterns were investigated in mammary tissues of ewes infected by Staphylococcus aureus, Streptococcus uberis, or Mycoplasma agalactiae, with a combined approach including immunohistochemistry, immune-colocalization, and fluorescent in situ hybridization...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28666865/different-%C3%AE-glucans-improve-the-growth-performance-and-bacterial-resistance-in-nile-tilapia
#9
Fabiana Pilarski, Carlos Alberto Ferreira de Oliveira, Fernanda Patrícia Brito Darpossolo de Souza, Fábio Sabbadin Zanuzzo
The role of glucan as a biologically active immunomodulator has been well documented for more than 40 years. However, the wide diversity of β-glucan forms and the extraction process has implications for the benefits of these compounds. Biorigin developed two samples of β-glucans using different biotechnological processes. Thus, in the present study, we fed Nile tilapia (Oreochromis niloticus) diets containing these two β-glucan molecules (BG01 and BG02) for 30 days prior to bacterial infection with Streptococcus agalactiae...
June 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28666619/development-of-a-multiplex-pcr-for-identification-of-%C3%AE-hemolytic-streptococci-relevant-to-human-infections-and-serotype-distribution-of-invasive-streptococcus-agalactiae-in-thailand
#10
Anusak Kerdsin, Rujirat Hatrongjit, Shigeyuki Hamada, Yukihiro Akeda, Marcelo Gottschalk
A multiplex polymerase chain reaction (mPCR) was developed for simultaneous detection (single reaction) of genes specific to five frequent clinically relevant β-hemolytic streptococcal species: Streptococcus pyogenes (Spy1258), Streptococcus agalactiae (cfb and cpn60), Streptococcus dysgalactiae subsp. equisimilis (16S-23S intergenic spacer) , S. equi subsp. zooepidemicus (esaA and sorD), and Streptococcus anginosus group (moaC). No cross-reaction was observed with other bacterial species. This test was validated and successfully used with 725 clinical isolates involved in pathological conditions in Thailand and collected between March 2014 and December 2015...
June 27, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28634536/telavancin-a-novel-semisynthetic-lipoglycopeptide-agent-to-counter-the-challenge-of-resistant-gram-positive-pathogens
#11
REVIEW
Biswadeep Das, Chayna Sarkar, Debasmita Das, Amit Gupta, Arnav Kalra, Shubham Sahni
Telavancin (TD-6424), a semisynthetic lipoglycopeptide vancomycin-derivative, is a novel antimicrobial agent developed by Theravance for overcoming resistant Gram-positive bacterial infections, specifically methicillin-resistant Staphylococcus aureus (MRSA). The US Food and Drug Administration (USFDA) had approved telavancin in 2009 for the treatment of complicated skin and skin structure infections (cSSSIs) caused by Gram-positive bacteria, including MRSA (S. aureus, Streptococcus agalactiae, Streptococcus pyogenes, Streptococcus anginosus group, or Enterococcus faecalis)...
March 2017: Therapeutic Advances in Infectious Disease
https://www.readbyqxmd.com/read/28633864/perinatal-group-b-streptococcal-infections-virulence-factors-immunity-and-prevention-strategies
#12
REVIEW
Jay Vornhagen, Kristina M Adams Waldorf, Lakshmi Rajagopal
Group B streptococcus (GBS) or Streptococcus agalactiae is a β-hemolytic, Gram-positive bacterium that is a leading cause of neonatal infections. GBS commonly colonizes the lower gastrointestinal and genital tracts and, during pregnancy, neonates are at risk of infection. Although intrapartum antibiotic prophylaxis during labor and delivery has decreased the incidence of early-onset neonatal infection, these measures do not prevent ascending infection that can occur earlier in pregnancy leading to preterm births, stillbirths, or late-onset neonatal infections...
June 17, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28622857/streptococcus-suis-serotype-9-strain-gz0565-contains-a-type-vii-secretion-system-putative-substrate-esxa-that-contributes-to-bacterial-virulence-and-a-vanz-like-gene-that-confers-resistance-to-teicoplanin-and-dalbavancin-in-streptococcus-agalactiae
#13
Liying Lai, Jiao Dai, Huanyu Tang, Shouming Zhang, Chunyan Wu, Wancen Qiu, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu
Streptococcus suis (SS), an important pathogen for pigs, is not only considered as a zoonotic agent for humans, but is also recognized as a major reservoir of antimicrobial resistance contributing to the spread of resistance genes to other pathogenic Streptococcus species. In addition to serotype 2 (SS2), serotype 9 (SS9) is another prevalent serotype isolated from diseased pigs. Although many SS strains have been sequenced, the complete genome of a non-SS2 virulent strain has been unavailable to date. Here, we report the complete genome of GZ0565, a virulent strain of SS9, isolated from a pig with meningitis...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28606365/estimate-of-the-economic-impact-of-mastitis-a-case-study-in-a-holstein-dairy-herd-under-tropical-conditions
#14
Juliana L B Guimarães, Maria A V P Brito, Carla C Lange, Márcio R Silva, João B Ribeiro, Letícia C Mendonça, Juliana F M Mendonça, Guilherme N Souza
The aim of this study was to estimate the economic impact of mastitis at the herd level and the weight (percent) of the components of this impact in a Holstein dairy herd under tropical conditions. Three estimates of the economic impact of mastitis were performed. In estimates 1 and 2 the real production and economic indices from February 2011 to January 2012 were considered. In the estimate 1, indices for mastitis classified as ideal were considered, whereas in the estimate 2, the mastitis indices used were those recorded at the farm and at Holstein Cattle Association of Minas Gerais State database (real indices)...
July 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28602737/identification-and-characterization-of-a-mannose-binding-lectin-from-nile-tilapia-oreochromis-niloticus
#15
Liangliang Mu, Xiaoxue Yin, Jing Liu, Liting Wu, Xia Bian, Yuhong Wang, Jianmin Ye
Mannose-binding lectin (MBL) is a pattern recognition protein that plays an important role in innate immunity capable of activating the lectin pathway of the complement system. In this study, a MBL homologue (OnMBL) was identified from Nile tilapia (Oreochromis niloticus) and characterized at expression and agglutination functional levels. The open reading frame of OnMBL is 687 bp of nucleotide sequence encoding polypeptides of 228 amino acids. The deduced amino acid sequence is highly homology to teleost and similar to mammalian MBL, containing a canonical collagen-like region, a carbohydrate recognition domain and a neck region...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602734/molecular-characterization-and-gene-expression-of-cathepsin-l-in-nile-tilapia-oreochromis-niloticus
#16
Fu-Rui Liang, Yue-Hui Hong, Cong-Cong Ye, Hailin Deng, Jian-Ping Yuan, Yun-Fang Hao, Jiang-Hai Wang
Cathepsin L (CatL) has been widely known for its involvement in the innate immunity. However, it still remains poorly understand how CatL modulates the immune system of teleosts. Moreover, the CatL of Nile tilapia (NtCatL) has not been cloned or characterized. In this study, the gene encoding NtCatL was cloned, and was characterized by bioinformatics analysis, heterologous expression and protease activity assay. The coding sequence of NtCatL is 1017 bp in length and encodes 338 amino acid residues with a predicted molecular weight of 38...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602733/efficacy-of-viable-bacillus-pumilus-isolated-from-farmed-fish-on-immune-responses-and-increased-disease-resistance-in-nile-tilapia-oreochromis-niloticus-laboratory-and-on-farm-trials
#17
Prapansak Srisapoome, Nonthawit Areechon
Applications of viable Bacillus pumilus AQAHBS01 isolated from Nile tilapia farms as probiotics were studied in both laboratory and farm conditions. In the laboratory, feeding fish (approximately 50 g) with feed containing viable B. pumilus at concentrations of 1 × 10(7)-10(9) colony forming units (CFU)/kg elevated fish immune responses, as indicated by their phagocytic activity and superoxide anion levels, and led to more effective disease resistance against Streptococcus agalactiae. However, when these concentrations were applied to Nile tilapia cultures growing in cage culture systems, only B...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28601455/within-herd-prevalence-of-intramammary-infection-caused-by-mycoplasma-bovis-and-associations-between-cow-udder-health-milk-yield-and-composition
#18
Anri A E Timonen, Jørgen Katholm, Anders Petersen, Kerli Mõtus, Piret Kalmus
Subclinical mastitis is one of the major health problems in dairy herds due to decreased milk production and reduced milk quality. The aim of this study was to examine the within-herd prevalence of subclinical intramammary infection caused by Mycoplasma bovis and to evaluate associations between M. bovis and cow daily milk yield, udder health, and milk composition. Individual cow composite milk samples (n = 522) were collected from all lactating dairy cows in 1 Estonian dairy farm in November 2014. Daily milk yield, days in milk, and parity were recorded...
June 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28601447/phenotypic-and-genotypic-antimicrobial-susceptibility-pattern-of-streptococcus-spp-isolated-from-cases-of-clinical-mastitis-in-dairy-cattle-in-poland
#19
E Kaczorek, J Małaczewska, R Wójcik, W Rękawek, A K Siwicki
Mastitis of dairy cattle is one of the most frequently diagnosed diseases worldwide. The main etiological agents of mastitis are bacteria of the genus Streptococcus spp., in which several antibiotic resistance mechanisms have been identified. However, detailed studies addressing this problem have not been conducted in north-eastern Poland. Therefore, the aim of our study was to analyze, on phenotypic and genotypic levels, the antibiotic resistance pattern of Streptococcus spp. isolated from clinical cases of mastitis from dairy cattle in this region of Poland...
June 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28600854/impact-of-intramammary-tilmicosin-infusion-as-a-dry-cow-therapy
#20
M Mohammadsadegh
Three hundred subclinically infected quarters of 259 Holstein cows infected with gram-positive bacteria were selected via quota sampling based on the California Mastitis Test (CMT) result and were divided randomly and equally into treatment and test groups. Quarters of test group (n = 150 in 128 cows) were treated with an intramammary infusion of tilmicosin, and quarters of the control group (n = 150 in 131 cows) were treated with cloxacillin as a traditional intramammary infusion of dry cow (DC) ointment...
June 10, 2017: Journal of Veterinary Pharmacology and Therapeutics
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