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https://www.readbyqxmd.com/read/28930847/guillain-barr%C3%A3-like-axonal-polyneuropathy-associated-with-toscana-virus-infection-a-case-report
#1
Eugenia Rota, Nicola Morelli, Paolo Immovilli, Paola De Mitri, Donata Guidetti
RATIONALE: Numerous cases of post-infectious Guillain-Barré syndrome (GBS) have been reported in the literature. Toscana virus (TOSV) is an arthropod-borne emerging pathogen in the Mediterranean area. PATIENT CONCERNS: A 40-year-old male patient was admitted to hospital for acute facial weakness, associated to numbness paraesthesias at lower and upper limbs. The neurological examination revealed facial diplegia and reduced tendon reflexes. The nerve conduction studies documented an acute motor and sensory axonal neuropathy (AMSAN); the lumbar puncture detected albuminocytologic dissociation...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28928949/plasmapheresis-in-neurological-disorders-six-years-experience-from-university-clinical-center-tuzla
#2
Osman Sinanović, Sanela Zukić, Adnan Burina, Nermina Pirić, Renata Hodžić, Mirza Atić, Mirna Alečković-Halilović, Enisa Mešić
BACKGROUND: Therapeutic plasma exchange (TPE) is an extracorporeal blood purification technique that is designed to remove substances with a large molecular weight. The TPE procedure includes removal of antibodies, alloantibodies, immune complexes, monoclonal protein, toxins or cytokines, and involves the replenishment of a specific plasma factor. The aim of the study was to describe the clinical response to TPE in various neurological patients, and to assess the clinical response to this therapy...
2017: F1000Research
https://www.readbyqxmd.com/read/28928763/comparative-mapping-and-candidate-gene-analysis-of-ssiia-associated-with-grain-amylopectin-content-in-barley-hordeum-vulgare-l
#3
Xiangyun Fan, Juan Zhu, Wenbin Dong, Yuandong Sun, Chao Lv, Baojian Guo, Rugen Xu
Amylopectin concentration in barley endosperm has important effects on grain quality and end-use. In this study, quantitative trait locus (QTL) analysis together with genome-wide association studies (GWAS) were performed to identify markers linked to grain amylopectin content respectively using a doubled haploid (DH) population of 178 lines and a collection of 185 diverse barley germplasms both genotyped by genotyping-by-sequencing (GBS). A stable QTL on chromosome 7H and 11 associated single nucleotide polymorphisms (SNPs) were detected...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28928416/zika-virus-infection-confers-protection-against-west-nile-virus-challenge-in-mice
#4
Ángela Vázquez-Calvo, Ana-Belén Blázquez, Estela Escribano-Romero, Teresa Merino-Ramos, Juan-Carlos Saiz, Miguel A Martín-Acebes, Nereida Jiménez de Oya
Flaviviruses are RNA viruses that constitute a worrisome threat to global human and animal health. Zika virus (ZIKV), which was initially reported to cause a mild disease, recently spread in the Americas, infecting millions of people. During this recent epidemic, ZIKV infection has been linked to serious neurological diseases and birth defects, specifically Guillain-Barrè syndrome (GBS) and microcephaly. Because information about ZIKV immunity remains scarce, we assessed the humoral response of immunocompetent mice to infection with three viral strains of diverse geographical origin (Africa, Asia and America)...
September 20, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28923133/genetic-evidence-for-a-novel-variant-of-the-pilus-island-1-backbone-protein-in-group-b-streptococcus
#5
Sarah Teatero, Alefiya Neemuchwala, Kunlin Yang, Janice Gomes, Taryn B T Athey, Irene Martin, Walter Demczuk, Allison McGeer, Nahuel Fittipaldi
PURPOSE: Pili contribute significantly to the pathogenesis of infection of group B Streptococcus (GBS) by facilitating adhesion and invasion of host cells. GBS pilin subunits (the backbone pilin protein, BP, and the ancillary pilin proteins, AP) as well as the specific enzymes required for pilus assembly are encoded by genes located in two separate genomic regions, known as pilus island 1 (PI-1) and PI-2. Our aim was to characterize the pilus profile of a collection of GBS isolates from metropolitan Toronto, Canada...
September 19, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28920845/modified-use-of-real-time-pcr-detection-of-group-b-streptococcus-in-pregnancy
#6
Ali A Rabaan, Justin V Saunar, Ali M Bazzi, Joan L Soriano
The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35-37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd-Hewitt enrichment broth cultures were examined daily for growth. Todd-Hewitt broth culture was also used for Xpert GBS...
September 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28919600/modified-blatchford-score-for-risk-stratification-in-adult-patient-with-nonvariceal-upper-gastrointestinal-haemorrhage-and-their-short-term-hospital-outcome
#7
M S Islam, M Z Uddin, M S Ali, M N Islam, M H Rahman, I H Robi, M M Haque, M W Rahman, M A Uddin, F Ahamed, M S Malek, M A Kabir, M S Rahman, A U Khan, F A Doel
Upper gastrointestinal hemorrhage (UGIH) is one of the most common and life-threatening gastrointestinal emergency. There are several risk scores for risk stratification in UGIB patients. The Modified Blatchford score, which relies only on clinical and laboratory parameters, is practical in the emergency setting The Modified Blatchford scoring system also known as Glasgow Blatchford Scoring (GBS) have been developed to stratify risk of non variceal upper gastrointestinal hemorrhage or need of medical or surgical intervention, endoscopic therapy...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28917117/anaerobic-granular-sludge-for-simultaneous-biomethanation-of-synthetic-wastewater-and-co-with-focus-on-the-identification-of-co-converting-microorganisms
#8
Yuhang Jing, Stefano Campanaro, Panagiotis Kougias, Laura Treu, Irini Angelidaki, Shicheng Zhang, Gang Luo
CO is a main component of syngas, which can be produced from the gasification of organic wastes and biomass. CO can be converted to methane by anaerobic digestion (AD), however, it is still challenging due to its toxicity to microorganisms and limited knowledge about CO converting microorganisms. In the present study, anaerobic granular sludge (AGS) was used for the simultaneous biomethanation of wastewater and CO. Batch experiments showed that AGS tolerated CO partial pressure as high as 0.5 atm without affecting its ability for synthetic wastewater degradation, which had higher tolerance of CO compared to suspended sludge (less than 0...
September 9, 2017: Water Research
https://www.readbyqxmd.com/read/28916459/variation-in-campylobacter-jejuni-culturability-in-presence-of-acanthamoeba-castellanii-neff
#9
María Reyes-Batlle, Cecilia Girbau, Atteneri López-Arencibia, Ines Sifaoui, Aitor Rizo Liendo, Carlos J Bethencourt Estrella, Ana B García Méndez, Olfa Chiboub, Soumaya Hajaji, Aurora Fernández-Astorga, Basilio Valladares, Enrique Martínez-Carretero, José E Piñero, Jacob Lorenzo-Morales
Free-living amoebae (FLA) are protozoa that are widely distributed in the environment mainly in water and soil related habitats. These amoebae have also been reported to be associated with some bacterial pathogens for humans such as Campylobacter spp. The species C. jejuni is the causative agent of about 90% of human campylobacteriosis cases worldwide and this disease may even end up in severe autoimmune sequelae as Guillain-Barré syndrome (GBS). In this study, the interactions between the strain Acanthamoeba castellanii Neff and Campylobacter jejuni was investigated...
September 12, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28916318/matrix-metalloproteinases-2-and-9-in-campylobacter-jejuni-induced-paralytic-neuropathy-resembling-guillain-barr%C3%A3-syndrome-in-chickens
#10
Kishan Kumar Nyati, Kashi Nath Prasad, Vinita Agrawal, Nuzhat Husain
Inflammation in Guillain-Barré syndrome (GBS) is manifested by changes in matrix metalloproteinase (MMP) and pro-inflammatory cytokine expression. We investigated the expression of MMP-2, -9 and TNF-α and correlated it with pathological changes in sciatic nerve tissue from Campylobacter jejuni-induced chicken model for GBS. Campylobacter jejuni and placebo were fed to chickens and assessed for disease symptoms. Sciatic nerves were examined by histopathology and immunohistochemistry. Expressions of MMPs and TNF-α, were determined by real-time PCR, and activities of MMPs by zymography...
September 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28916188/mast-cell-chymase-decreases-the-severity-of-group-b-streptococcus-infections
#11
Claire Gendrin, Nicholas J Shubin, Erica Boldenow, Sean Merillat, Morgan Clauson, Danny Power, Kelly S Doran, Magnus Abrink, Gunnar Pejler, Lakshmi Rajagopal, Adrian M Piliponsky
BACKGROUND: Group B Streptococcus (GBS) or Streptococcus agalactiae are ß-hemolytic, Gram-positive bacteria that colonize the lower genital tract of women and are frequently associated with infections during pregnancy. Innate immune defenses are critical for controlling GBS dissemination and systemic infection. Mast cells are resident sentinel cells that come into contact with pathogens early during colonization and infection. OBJECTIVE: We aimed to investigate the contribution of chymase to systemic GBS infection and rates of preterm birth...
September 12, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28915793/the-cacao-criollo-genome-v2-0-an-improved-version-of-the-genome-for-genetic-and-functional-genomic-studies
#12
X Argout, G Martin, G Droc, O Fouet, K Labadie, E Rivals, J M Aury, C Lanaud
BACKGROUND: Theobroma cacao L., native to the Amazonian basin of South America, is an economically important fruit tree crop for tropical countries as a source of chocolate. The first draft genome of the species, from a Criollo cultivar, was published in 2011. Although a useful resource, some improvements are possible, including identifying misassemblies, reducing the number of scaffolds and gaps, and anchoring un-anchored sequences to the 10 chromosomes. METHODS: We used a NGS-based approach to significantly improve the assembly of the Belizian Criollo B97-61/B2 genome...
September 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28915414/evaluation-of-surgical-decision-making-and-resulting-outcome-in-patients-with-highly-eloquent-glioblastoma-results-of-a-multicenter-assessment
#13
Jan Coburger, Mirjam Renovanz, Oliver Ganslandt, Florian Ringel, Christian Rainer Wirtz, Javier Segovia von Riehm
INTRODUCTION: Treatment of glioblastoma(GB) patients amenable only for a subtotal resection(STR) is controversial. Since outcome of patients is affected by surgical management, our aim was to assess surgical decision making and resulting outcome in patients with highly eloquent GBs. PATIENTS AND METHODS: We retrospectively assessed GB patients with intended sub-total resection (STR) or stereotactic biopsy (STX) of 3 neurooncological centers operated between 2008 and 2013...
September 8, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28914529/a-well-hidden-grain-boundary-in-the-monolayer-mos2-formed-by-a-two-dimensional-core-shell-growth-mode
#14
Wenting Zhang, Yue Lin, Qi Wang, Weijie Li, Zhifeng Wang, Jiangluqi Song, Xiaodong Li, Lijie Zhang, Lixin Zhu, XiaoLiang Xu
Guided by the hexagonal lattice symmetry, triangle and hexagon are the most basic morphological unit for the two-dimensional (2D) transition metal dichalcogenides (TMDs) synthesized by chemical vapor deposition (CVD). Also, it is widely acknowledged that these units start from the single nucleation site and then grow epitaxially. Accordingly, the triangular monolayer (ML) samples are generally considered as single crystals. Here, we report a 2D core-shell growth mode in the CVD process for ML-MoS2, which leads to one kind of "pseudo" single crystal triangles containing triangular outline grain boundaries (TO-GBs)...
September 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/28913899/genotyping-by-sequencing-through-transcriptomics-implementation-in-a-range-of-crop-species-with-varying-reproductive-habits-and-ploidy-levels
#15
Michelle M Malmberg, Luke W Pembleton, Rebecca C Baillie, Michelle C Drayton, Shimna Sudheesh, Sukhjiwan Kaur, Hiroshi Shinozuka, Preeti Verma, German C Spangenberg, Hans D Daetwyler, John W Forster, Noel O I Cogan
The application of genomics in crops has the ability to significantly improve genetic gain for agriculture. Many marker-dense tools have been developed but few have seen broad adoption in plant genomics due to issues of significant variations of genome size, levels of ploidy, single nucleotide polymorphism (SNP) frequency and reproductive habit. When combined with limited breeding activities, small research communities and scant sequence resources, the suitability of popular systems is often sub-optimal and routinely fails to effectively balance cost-effectiveness and sample throughput...
September 15, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28912785/genotyping-by-sequencing-gbs-revealed-molecular-genetic-diversity-of-iranian-wheat-landraces-and-cultivars
#16
Hadi Alipour, Mohammad R Bihamta, Valiollah Mohammadi, Seyed A Peyghambari, Guihua Bai, Guorong Zhang
Background: Genetic diversity is an essential resource for breeders to improve new cultivars with desirable characteristics. Recently, genotyping-by-sequencing (GBS), a next-generation sequencing (NGS) technology that can simplify complex genomes, has now be used as a high-throughput and cost-effective molecular tool for routine breeding and screening in many crop species, including the species with a large genome. Results: We genotyped a diversity panel of 369 Iranian hexaploid wheat accessions including 270 landraces collected between 1931 and 1968 in different climate zones and 99 cultivars released between 1942 to 2014 using 16,506 GBS-based single nucleotide polymorphism (GBS-SNP) markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28904998/parallel-evolution-of-group-b-streptococcus-hypervirulent-clonal-complex-17-unveils-new-pathoadaptive-mutations
#17
Alexandre Almeida, Isabelle Rosinski-Chupin, Céline Plainvert, Pierre-Emmanuel Douarre, Maria J Borrego, Claire Poyart, Philippe Glaser
Group B Streptococcus (GBS) is a commensal of the gastrointestinal and genitourinary tracts, while a prevailing cause of neonatal disease worldwide. Of the various clonal complexes (CCs), CC17 is overrepresented in GBS-infected newborns for reasons that are still largely unknown. Here, we report a comprehensive genomic analysis of 626 CC17 isolates collected worldwide, identifying the genetic traits behind their successful adaptation to humans and the underlying differences between carriage and clinical strains...
September 2017: MSystems
https://www.readbyqxmd.com/read/28900347/campylobacter-from-microbiology-to-prevention
#18
REVIEW
A Facciolà, R Riso, E Avventuroso, G Visalli, S A Delia, P Laganà
In last years, Campylobacter spp has become one of the most important foodborne pathogens even in high-income countries. Particularly, in Europe, Campylobacteriosis is, since 2005, the foodborne disease most frequently notified and the second in USA, preceded by the infection due to Salmonella spp. Campylobacter spp is a commensal microorganism of the gastrointestinal tract of many wild animals (birds such as ducks and gulls), farm animals (cattle and pigs) and companion animals (such as dogs and cats) and it is responsible for zoonoses...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28899886/brainstem-encephalitis-and-acute-polyneuropathy-associated-with-hepatitis-e-infection
#19
Omar Jabbar Salim, Amy Davidson, Kathy Li, John Paul Leach, Craig Heath
A 59-year-old man presented with feverish illness. His Glasgow Coma Scale was 15, had reduced visual acuity in the left eye with partial left ptosis and mild left hemiparesis with an extensor left plantar. Over 48 hours, he accrued multiple cranial nerves palsies and progressed to a flaccid paralysis necessitating admission to an intensive care unit.Cerebrospinal fluid (CSF) study showed 20 lymphocytes and raised protein. Viral and bacterial PCRs were negative. Samples for Lyme, blood-borne viruses, syphilis and autoantibodies were also negative...
September 11, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28895946/dna-sip-based-genome-centric-metagenomics-identifies-key-long-chain-fatty-acid-degrading-populations-in-anaerobic-digesters-with-different-feeding-frequencies
#20
Ryan M Ziels, Diana Z Sousa, H David Stensel, David A C Beck
Fats, oils and greases (FOG) are energy-dense wastes that can be added to anaerobic digesters to substantially increase biomethane recovery via their conversion through long-chain fatty acids (LCFAs). However, a better understanding of the ecophysiology of syntrophic LCFA-degrading microbial communities in anaerobic digesters is needed to develop operating strategies that mitigate inhibitory LCFA accumulation from FOG. In this research, DNA stable isotope probing (SIP) was coupled with metagenomic sequencing for a genome-centric comparison of oleate (C18:1)-degrading populations in two anaerobic codigesters operated with either a pulse feeding or continuous-feeding strategy...
September 12, 2017: ISME Journal
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