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https://www.readbyqxmd.com/read/29785615/molecular-characterization-of-proton-beam-induced-mutations-in-soybean-using-genotyping-by-sequencing
#1
Woon Ji Kim, Jaihyunk Ryu, Juhyun Im, Sang Hun Kim, Si-Yong Kang, Jeong-Hee Lee, Sung-Hwan Jo, Bo-Keun Ha
Proton beam irradiation is a next-generation technique to develop mutant crop varieties. The mutagenic effects and molecular mechanisms of radiation are important multi-disciplinary research subjects. This study was conducted to investigate the types of mutations induced in the soybean genome by proton beam irradiation. In total, 22 plants, including 10 M2 plants treated with proton beam irradiation at 118 and 239 Gy, each, and two wild-type plants (Daepung) were sequenced by genotyping-by-sequencing (GBS)...
May 21, 2018: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29780225/sleep-and-psychiatric-abnormalities-in-gullian-barr%C3%A3-syndrome
#2
Wafik Said Bahnasy, Yasser Abo Elfotoh El-Heneedy, Ahmed Mohamed El-Shamy, Marwa Yassin Badr, Reham Ahmed Amer, Ibrahim Salah Eldeen Ibrahim
Background: The sensori-motor manifestations of Guillain Barré Syndrome (GBS) are usually severe enough to mask the psychiatric and sleep problems which are in need for more attention for better functional outcome. Methods: This study was performed on 20 GBS patients and 10 healthy controls. Patients were evaluated initially before immunotherapy using the Overall Disability Sum Score (ODSS), Neuropathy Pain Scale (NPS), Hamilton Anxiety Scale (HAS), Montgomery-Åsberg Depression Rating Scale (MADRS) and one-night polysomnography (PSG) followed by the multiple sleep latency test (MSLT) to evaluate the mean sleep latencies...
2018: The Egyptian journal of neurology, psychiatry and neurosurgery
https://www.readbyqxmd.com/read/29780224/correlative-study-between-c-reactive-protein-clinical-severity-and-nerve-conduction-studies-in-guillain-barr%C3%A3-syndrome
#3
Yosria A Altaweel, Sawsan Abdelaziz, Hala A Fathy, Shimaa AbdelBadea
Background: Guillain-Barre' syndrome (GBS) is a serious autoimmune disorder in which the immune system attacks healthy nerve cells of the peripheral nervous system causing polyradiculoneuropathy which leads to weakness, numbness, and tingling, and can eventually cause paralysis. Autoimmune conditions like GBS can induce a high level of inflammation resulting in an increase in the C-reactive protein( CRP) production. The aim of this study is to assess the relationship between CRP level and the clinical severity as well as the electrophysiological findings of nerve conduction studies in patients with GBS...
2018: The Egyptian journal of neurology, psychiatry and neurosurgery
https://www.readbyqxmd.com/read/29779827/zika-virus-report-from-the-task-force-on-tropical-diseases-by-the-world-federation-of-societies-of-intensive-and-critical-care-medicine
#4
Gisele Sampaio Silva, Guy A Richards, Tim Baker, Jorge Hidalgo, Juan Ignacio Silesky Jiménez, Pravin Amin
Zika fever is an acute infectious disease caused by the Zika virus (ZIKV), an RNA virus belonging to the genus Flavivirus. ZIKV can be transmitted by bites from Aedes aegypti and Aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. A total of 50 territories and countries in the Americas had reported ZIKV infections. For those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fever and conjunctivitis lasting up to seven days...
April 5, 2018: Journal of Critical Care
https://www.readbyqxmd.com/read/29774036/the-pattern-and-distribution-of-induced-mutations-in-j-curcas-using-reduced-representation-sequencing
#5
Fatemeh Maghuly, Stephan Pabinger, Julie Krainer, Margit Laimer
Mutagenesis in combination with Genotyping by Sequencing (GBS) is a powerful tool for introducing variation, studying gene function and identifying causal mutations underlying phenotypes of interest in crop plant genomes. About 400 million paired-end reads were obtained from 82 ethylmethane sulfonate (EMS) induced mutants and 14 wild-type accessions of Jatropha curcas for the detection of Single Nucleotide Polymorphisms (SNPs) and Insertion/Deletions (InDels) by two different approaches (nGBS and ddGBS) on an Illumina HiSeq 2000 sequencer...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29772575/therapeutic-effect-of-cd4-cd25-regulatory-t-cells-amplified-in-vitro-on-experimental-autoimmune-neuritis-in-rats
#6
Feng-Jie Wang, Dan Cui, Wei-Dong Qian
BACKGROUND/AIMS: This study aimed to explore whether the adoptive transfusion of autologous CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) has a therapeutic effect on Experimental autoimmune neuritis (EAN) model rats, and it provides new experimental and theoretical bases for the immunotherapy of Guillain-Barre syndrome (GBS). METHODS: CD4+CD25+ Tregs were sorted from the spleens of rats using immunomagnetic bead separation techniques combined with flow cytometry...
May 14, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29770902/high-isolation-rate-and-multidrug-resistance-tendency-of-penicillin-susceptible-group-b-streptococcus-with-reduced-ceftibuten-susceptibility-in-japan
#7
Hirotsugu Banno, Kouji Kimura, Tomomi Seki, Wanchun Jin, Jun-Ichi Wachino, Keiko Yamada, Noriyuki Nagano, Yoshichika Arakawa
Group B Streptococcus (GBS) clinical isolates with reduced penicillin susceptibility (PRGBS) have emerged through acquisition of amino acid substitutions in penicillin-binding protein 2X (PBP2X). Moreover, we also reported the emergence of penicillin-susceptible GBS clinical isolates with reduced ceftibuten susceptibility (CTBr PSGBS) due to amino acid substitutions in PBPs. However, whether or not these amino acid substitutions are responsible for the reduced ceftibuten susceptibility (RCTBS) profile remains unclear...
May 17, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29763724/influence-of-environmental-and-genotypic-factors-on-biofilm-formation-by-clinical-isolates-of-group-b-streptococci
#8
Shuping Nie, Xuedong Lu, Yan-Wei Hu, Lei Zheng, Qian Wang
Group B Streptococcus (GBS) colonizes the gastrointestinal and urogenital tracts of approximately 30% of women, and it can cause sepsis and meningitis in neonates. GBS has been shown to form biofilms in vitro, but the effects of environmental and genotypic factors upon GBS biofilm formation are unclear. The aim of the present study was to optimize culture conditions for enhanced GBS biofilm production. Furthermore, this study also investigated the influences of strain lineage, pilus profile, and isolation source on GBS biofilm formation...
May 12, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29762015/preferential-pt-nanocluster-seeding-at-grain-boundary-dislocations-in-polycrystalline-monolayer-mos-2
#9
Shanshan Wang, Hidetaka Sawada, Xiaoyu Han, Si Zhou, Sha Li, Zheng Xiao Guo, Angus I Kirkland, Jamie H Warner
We show that Pt nanoclusters preferentially nucleate along the grain boundaries (GBs) in polycrystalline MoS2 monolayer films, with dislocations acting as the seed site. Atomic resolution studies by aberration-corrected annular dark-field scanning transmission electron microscopy reveal periodic spacing of Pt nanoclusters with dependence on GB tilt angles and random spacings for the antiphase boundaries ( i.e., 60°). Individual Pt atoms are imaged within the dislocation core sections of the GB region, with various positions observed, including both the substitutional sites of Mo and the hollow center of the octahedral ring...
May 15, 2018: ACS Nano
https://www.readbyqxmd.com/read/29761674/screening-arab-israeli-pregnant-women-for-group-b-streptococcus-by-the-amplivue-gbs-assay-are-the-rates-higher-than-the-national-average
#10
Marwan Hakim, Adel Jabour, Miriam Anton, Meggie Hakim, Sahar Kheirallah
BACKGROUND: The recommendation of the U.S. Centers for Disease Control and Prevention regarding universal screening for Group B Streptococcus (GBS) at 35-37 weeks gestational age in pregnancy is not accepted in Israel. The National Council for Obstetrics, Neonatology and Genetics recommends intrapartum prophylaxis, mainly based on risk factors, to prevent early neonatal GBS infection. This policy is based on past studies demonstrating low colonization rates of the bacteria in Israeli pregnant women and very low neonatal sepsis rates...
May 2018: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/29761123/zika-virus-and-guillain-barr%C3%A3-syndrome-in-bangladesh
#11
Corine H GeurtsvanKessel, Zhahirul Islam, Md Badrul Islam, Sandra Kamga, Nowshin Papri, David A M C van de Vijver, Chantal Reusken, Ramona Mogling, Astrid P Heikema, Israt Jahan, Florence K Pradel, Rebecca L Pavlicek, Quazi D Mohammad, Marion P G Koopmans, Bart C Jacobs, Hubert P Endtz
Objective: Previous studies have associated Guillain-Barré syndrome (GBS) with Zika virus (ZIKV) outbreaks in South America and Oceania. In Asia, ZIKV is known to circulate widely, but the association with Guillain-Barré syndrome is unclear. We investigated whether endemic ZIKV infection is associated with the development of GBS. Methods: A prospective study was conducted from 2011 to 2015 in Bangladesh. A total of 418 patients and 418 healthy family controls were included in the study...
May 2018: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/29761013/guillain-barr%C3%A3-syndrome-mimics
#12
Sai Leong Tham, Kalpana Prasad, Thirugnanam Umapathi
The Brighton Collaboration criteria have standardized the clinical and laboratory-supported diagnosis of Guillain-Barré syndrome (GBS) and Miller Fisher syndrome (MFS) in a way that is applicable in many parts of the world with variable resources. The caveat within the criteria, "absence of an identified alternative diagnosis for weakness" makes GBS a diagnosis of exclusion. Accurate diagnosis of GBS requires a good understanding of an updated, locally contextualised list of mimics, and features that distinguish them from GBS...
May 2018: Brain and Behavior
https://www.readbyqxmd.com/read/29759069/guillain-barr%C3%A3-syndrome-risk-among-individuals-infected-with-zika-virus-a-multi-country-assessment
#13
Luis Mier-Y-Teran-Romero, Mark J Delorey, James J Sejvar, Michael A Johansson
BACKGROUND: Countries with ongoing outbreaks of Zika virus have observed a notable rise in reported cases of Guillain-Barré syndrome (GBS), with mounting evidence of a causal link between Zika virus infection and the neurological syndrome. However, the risk of GBS following a Zika virus infection is not well characterized. In this work, we used data from 11 locations with publicly available data to estimate the risk of GBS following an infection with Zika virus, as well as the location-specific incidence of infection and the number of suspect GBS cases reported per infection...
May 15, 2018: BMC Medicine
https://www.readbyqxmd.com/read/29756592/three-year-follow-up-study-in-patients-with-guillain-barr%C3%A3-syndrome
#14
Vesna Martic, Ivo Bozovic, Ivana Berisavac, Ivana Basta, Stojan Peric, Milica Babic, Sonja Lukic Rajic, Bogdan Bjelica, Olivera Stojiljkovic Tamas, Aleksandar Stojanov, Marija Grunauer, Mina Cobeljic, Nenad Komatina, Vanja Djuric, Milutin Petrovic, Balsa Vujovic, Aleksandra Dominovic Kovacevic, Gordana Djordjevic, Dejana Jovanovic, Zorica Stevic
A majority of patients with Guillain-Barré syndrome (GBS) have tendency of a good recovery. Our aim was to evaluate the outcome of the disease 1 and 3 years after GBS symptom onset. METHODS: During 2014, GBS was diagnosed in 82 patients in seven tertiary healthcare centers. Neurological follow-up was conducted in 57 (70%) patients after 1 year, and in 54 (66%) after 3 years. Functional disability was estimated according to the GBS disability scale (GDS), with a score of 0-3 indicating mild disability and a score of 4-6 indicating severe disability during acute phase, whereas a score >1 indicated poor recovery on follow-ups...
May 2018: Canadian Journal of Neurological Sciences. le Journal Canadien des Sciences Neurologiques
https://www.readbyqxmd.com/read/29750801/streptococcus-agalactiae-carriage-among-pregnant-women-living-in-rio-de-janeiro-brazil-over-a-period-of-eight-years
#15
Ana Caroline N Botelho, Juliana G Oliveira, Andreia P Damasco, Késia T B Santos, Ana Flávia M Ferreira, Gabriel T Rocha, Penélope S Marinho, Rita B G Bornia, Tatiana C A Pinto, Marco A Américo, Sergio E L Fracalanzza, Lúcia M Teixeira
Group B Streptococcus (GBS) carriage by pregnant women is the primary risk factor for early-onset GBS neonatal sepsis. Intrapartum antibiotic prophylaxis (IAP) can prevent this transmission route, and two main approaches are recommended to base the selection of pregnant women to be submitted to IAP: the risk-based and the culture-based strategies. In Brazil, compliance to such recommendations is poor, and not much is known about GBS carriage. In the present study, 3,647 pregnant women living in Rio de Janeiro State, Brazil, were screened for GBS anogenital colonization, over a period of 8 years (2008-2015)...
2018: PloS One
https://www.readbyqxmd.com/read/29745668/temperature-induced-self-assembly-of-the-group-b-streptococcus-gbs-fusion-antigen-gbs-nn
#16
Fabrice Rose, Silke Roovers, Mathias Fanø, Stine Harloff-Helleberg, Jacob Judas Kain Kirkensgaard, Kim Hejnaes, Per Fischer, Camilla Foged
Group B streptococcus (GBS) is a leading cause of serious bacterial neonatal infections worldwide, which provides an unmet medical need for a globally effective vaccine. The recombinant GBS fusion antigen GBS-NN contains the N-terminal regions of the GBS Rib and Alpha C proteins. It shows promising immunogenicity eliciting protective immunity in mice and encouraging results in early human clinical trials. Understanding the physical stability of GBS-NN containing conformational B-cell epitopes is crucial to ensure optimal vaccine stability, efficacy and safety...
May 10, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29744498/anomalous-diffusion-of-water-molecules-at-grain-boundaries-in-ice-i-h
#17
Pedro Augusto Franco Pinheiro Moreira, Roberto Gomes de Aguiar Veiga, Ingrid de Almeida Ribeiro, Rodrigo Freitas, Julian Helfferich, Maurice de Koning
Using ab initio and classical molecular dynamics simulations, we study pre-melting phenomena in pristine coincident-site-lattice grain boundaries (GBs) in proton-disordered hexagonal ice Ih at temperatures just below the melting point Tm. Concerning pre-melt-layer thicknesses, the results are consistent with the available experimental estimates for low-disorder impurity-free GBs. With regard to molecular mobility, the simulations provide a key new insight: the translational motion of the water molecules is found to be subdiffusive for time scales from ∼10 ns up to at least 0...
May 10, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29743772/atypical-neurological-manifestations-of-chikungunya-fever-two-case-reports
#18
Subodh Kumar Mahto, Pulin Kumar Gupta, Akanksha Singh, Ramesh Chand Meena
Chikungunya fever (CHIK) is an arboviral disease which is transmitted by a mosquito bite ( Aedes aegypti ). Fever, polyarthralgia, and maculopapular rash are hallmark clinical manifestations of this disease. These manifestations are self-liming and complete recovery is seen in most of the cases. However, atypical and lethal complications such as myocarditis, hepatitis, Guillain-Barre syndrome (GBS), and meningoencephalitis have been reported in few cases of CHIK. We hereby report two cases of chikungunya infection where rare and atypical manifestations of CHIK, i...
April 2018: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/29743241/new-insights-into-the-regulation-of-placental-growth-factor-gene-expression-by-the-transcription-factors-gcm1-and-dlx3-in-human-placenta
#19
Yueh-Ho Chiu, Ming-Ren Yang, Liang-Jie Wang, Ming-Hon Chen, Geen-Dong Chang, Hungwen Chen
Expression of placental growth factor (PGF) is closely associated with placental perfusion in early pregnancy. PGF is primarily expressed in placental trophoblasts, and its expression decreases in preeclampsia, associated with placental hypoxia. The transcription factors glial cells missing 1 (GCM1) and metal-regulatory transcription factor 1 (MTF1) have been implicated in the regulation of PGF gene expression through regulatory elements upstream and downstream of the PGF transcription start site, respectively...
May 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29740383/nervous-system-injury-and-neuroimaging-of-zika-virus-infection
#20
REVIEW
Shanshan Wu, Yu Zeng, Alexander Lerner, Bo Gao, Meng Law
In 2016, World Health Organization announced Zika virus infection and its neurological sequalae are a public health emergency of global scope. Preliminary studies have confirmed a relationship between Zika virus infection and certain neurological disorders, including microcephaly and Guillain-Barre syndrome (GBS). The neuroimaging features of microcephaly secondary to Zika virus infection include calcifications at the junction of gray-white matter and subcortical white matter with associated cortical abnormalities, diminution of white matter, large ventricles with or without hydrocephalus, cortical malformations, hypoplasia of cerebellum and brainstem, and enlargement of cerebellomedullary cistern...
2018: Frontiers in Neurology
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