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DNA silver nanocluster

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https://www.readbyqxmd.com/read/28858858/detection-of-large-deletion-in-human-brca1-gene-in-human-breast-carcinoma-mcf-7-cells-by-using-dna-silver-nanoclusters
#1
Yasaman Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali
Here we describe a label-free detection strategy for large deletion mutation in breast cancer related gene BRCA1 based on a DNA-silver Nanocluster Fluorescence upon Recognition-Induced Hybridization. The specific hybridization of DNA templated silver nanoclusters fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs Fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescent intensities. Under the optimal conditions, the fluorescent intensity of the DNA-AgNCs increased with the increasing deletion type within a dynamic range from 1...
August 31, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28852006/a-rapid-evaluation-of-acute-hydrogen-sulfide-poisoning-in-blood-based-on-dna-cu-ag-nanocluster-fluorescence-probe
#2
Yanjun Ding, Xingmei Li, Ceng Chen, Jiang Ling, Weichen Li, Yadong Guo, Jie Yan, Lagabaiyla Zha, Jifeng Cai
Hydrogen sulfide (H2S) is a highly toxic gas as a cause of inhalational death. Accurate detection of H2S poisoning concentration is valuable and vital for forensic workers to estimate the cause of death. But so far, it is no uniform and reliable standard method to measure sulfide concentrations in H2S poisoning blood for forensic identification. This study introduces a fluorescence sensing technique into forensic research, in which a DNA-templated copper/silver nanocluster (DNA-Cu/AgNCs) fluorescence probe has been proposed to selective detection of S(2-)...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28842019/a-label-free-sensitive-method-for-membrane-protein-detection-based-on-aptamer-and-agncs-transfer
#3
Zhenjun Liu, Wansong Chen, Yajin Han, Jiang Ouyang, Min Chen, Shengqiang Hu, Liu Deng, You-Nian Liu
Recently, membrane proteins have been considered as candidate cancer biomarkers and drug targets, due to their important roles in numerous physiological processes. Therefore, a facile, sensitive and quantitative detection of the membrane proteins is crucial for better understanding their roles in cancer cells and further validating their function in clinical research. We report a highly facile and sensitive detection method for membrane proteins on living cells in situ based on membrane protein-triggered release of cytosine (C)-rich single-stranded DNA (ssDNA) sequences, and the subsequent silver nanoclusters (AgNCs) transfer from polymer to C-rich ssDNA...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28800705/two-photon-imaging-of-3d-organization-of-bimetallic-auag-nanoclusters-in-dna-matrix
#4
Katarzyna Brach, Magdalena Waszkielewicz, Joanna Olesiak-Banska, Marek Samoc, Katarzyna Matczyszyn
We report on two-photon excitation properties of small silver-doped gold nanoclusters (AuAgNCs) and on their three-dimensional arrangement in a hybrid system composed of DNA liquid crystals (LCs) and AuAgNCs. UV-vis and fluorescence spectroscopy, transmission electron microscopy (TEM), and multiphoton excitation spectroscopy were used to characterize the nanoparticles. We show that AuAgNCs exhibit two-photon excited luminescence (2PL) emission and second-harmonic generation (SHG) and that these properties remain the same in liquid crystalline matrix...
August 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28709087/novel-electrochemiluminescence-of-silver-nanoclusters-fabricated-on-triplex-dna-scaffolds-for-label-free-detection-of-biothiols
#5
Lingyan Feng, Li Wu, Feifei Xing, Lianzhe Hu, Jinsong Ren, Xiaogang Qu
Electrochemiluminescence (ECL) of metal nanoclusters and their application have been widely reported due to the good biocompatibility, fascinating electrocatalytic activity and so on. Using DNA as synthesis template opens new opportunities to modulate the physical properties of AgNCs. Triplex DNA has been reported for the site-specific, homogeneous and highly stable silver nanoclusters (AgNCs) fabrication from our recent research. Here we further explore their extraordinary ECL properties and applications in biosensor utilization...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28662471/in-situ-grown-dna-nanotail-templated-silver-nanoclusters-enabling-label-free-electrochemical-sensing-of-terminal-deoxynucleotidyl-transferase-activity
#6
Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Chunnuan Du, Chunyang Zhai
A novel label-free electrochemical strategy was established based on the unique electro-catalytic activity of graphene oxide (GO)-supported terminal deoxynucleotidyl transferase (TdT)-generated C-rich DNA nanotail-templated silver nanoclusters (DNA-AgNCs). TdT can catalyze the deoxycytidine triphosphate (dCTP) to the 3'-OH terminus of single-stranded DNA (ssDNA) with no template; then, in the presence of Ag(I), TdT-generated C-rich DNA sequence was employed for the synthetic template of AgNCs because of the formed complexes of nitrogen atoms of cytosine based with silver atoms...
June 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28646761/regulation-on-the-aggregation-induced-emission-aie-of-dna-templated-silver-nanoclusters-by-bsa-and-its-hydrolysates
#7
Wei-Xian Wang, Yuqing Wu, Hong-Wei Li
The aggregation-induced emission (AIE) phenomenon of the metal nanoclusters (NCs) has been discovered quite recently, which is considered to be creative to improve the optical properties of NCs upon ligand aggregation. In the present study, we report an AIE system for double-stranded DNA-templated silver nanoclusters (AgNCs-dsDNA) by using bovine serum albumin (BSA) in solution, which induce a moderate emission enhancement of AgNCs (5-fold) and a blue-shift through a loosen interaction. In addition, significant luminescence enhancement (30-fold) is further stimulated by the addition of digestive enzyme to the system by altering the surface structure of the aggregated particles...
November 1, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28626564/integration-of-g-quadruplex-and-dna-templated-ag-ncs-for-nonarithmetic-information-processing
#8
Ru-Ru Gao, Tian-Ming Yao, Xiao-Yan Lv, Yan-Yan Zhu, Yi-Wei Zhang, Shuo Shi
To create sophisticated molecular logic circuits from scratch, you may not believe how common the building blocks can be and how diverse and powerful such circuits can be when scaled up. Using the two simple building blocks of G-quadruplex and silver nanoclusters (Ag NCs), we experimentally construct a series of multifunctional, label-free, and multi-output logic circuits to perform nonarithmetic functions: a 1-to-2 decoder, a 4-to-2 encoder, an 8-to-3 encoder, dual transfer gates, a 2 : 1 multiplexer, and a 1 : 2 demultiplexer...
June 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28548085/ultrafast-coherence-transfer-in-dna-templated-silver-nanoclusters
#9
Erling Thyrhaug, Sidsel Ammitzbøll Bogh, Miguel R Carro-Temboury, Charlotte Stahl Madsen, Tom Vosch, Donatas Zigmantas
DNA-templated silver nanoclusters of a few tens of atoms or less have come into prominence over the last several years due to very strong absorption and efficient emission. Applications in microscopy and sensing have already been realized, however little is known about the excited-state structure and dynamics in these clusters. Here we report on a multidimensional spectroscopy investigation of the energy-level structure and the early-time relaxation cascade, which eventually results in the population of an emitting state...
May 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28510342/constructing-a-robust-fluorescent-dna-stabilized-silver-nanocluster-probe-module-by-attaching-a-duplex-moiety
#10
Ruoyun Lin, Guangyu Tao, Yang Chen, Mingxing Chen, Feng Liu, Na Li
Fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs) have served as excellent luminescent probes and operation units in various applications. However, the fluorescence property of DNA-Ag NCs is very sensitive to elongation or modification of the DNA template, limiting the breadth of applications. In this work, we propose a strategy for constructing a robust fluorescent DNA-Ag NCs probe module by attaching a duplex moiety to the nanocluster-bearing sequence. The fluorescence intensity of the DNA-Ag NCs can be enhanced 90-fold upon hybridization of the elongated moiety...
May 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28500353/estimation-of-postmortem-interval-by-vitreous-potassium-evaluation-with-a-novel-fluorescence-aptasensor
#11
Yanjun Ding, Xingmei Li, Yadong Guo, Weicheng Duan, Jiang Ling, Lagabaiyla Zha, Jie Yan, Ying Zou, Jifeng Cai
Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K(+) ions. The linear range for K(+) ions was found to be 0...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28460331/a-novel-fluorometric-and-colorimetric-sensor-for-iodide-determination-using-dna-templated-gold-silver-nanoclusters
#12
Zihao Li, Ruiyi Liu, Guofeng Xing, Tong Wang, Siyu Liu
Determination of iodide ions has attracted considerable interest due to the importance and urgency. In the current work, we have developed a novel fluorescent and colorimetric platform for simple and selective detection of iodide ions based on the DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs). The detection system is based on the unique interaction between iodide ions and DNA-Au/Ag NCs, which shows an obvious fluorescence quenching with the addition of iodide ions and a good linear range from 0 to 10μmol/L for iodide ions...
January 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28425549/nicotinamide-adenine-dinucleotide-detection-based-on-silver-nanoclusters-stabilized-by-a-dumbbell-shaped-probe
#13
Hong-Ya Wang, Jin-Liang Ma, Bin-Cheng Yin, Bang-Ce Ye
We have developed a novel method for detecting nicotinamide adenine dinucleotide (NAD(+)) based on fluorescent silver nanoclusters (AgNCs) stabilized by a dumbbell-shaped DNA template containing two cytosine-loops joined in a dsDNA stem. The design involves two types of components: a dumbbell-shaped DNA template and three enzymes. In the presence of NAD(+) as a cofactor, Escherichia coli DNA ligase (E.coli DNA ligase) catalyzes template ligation to generate a sealed (no terminal nucleotides) dumbbell-shaped structure, preventing digestion by exonuclease III (Exo III) and exonuclease I (Exo I)...
April 20, 2017: Analyst
https://www.readbyqxmd.com/read/28405933/detection-of-p53-gene-mutation-single-base-mismatch-using-a-fluorescent-silver-nanoclusters
#14
Morteza Hosseini, Shiva Mohammadi, Yasaman-Sadat Borghei, Mohammad Reza Ganjali
P53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site...
April 13, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28367619/ratiometric-nanocluster-beacon-a-label-free-and-sensitive-fluorescent-dna-detection-platform
#15
Lei Ge, Ximei Sun, Qing Hong, Feng Li
Although researches until now have emphasized the influence of an oligonucleotide sequence on the fluorescence of oligonucleotide-stabilized silver nanoclusters (AgNCs), this influence has been explored as a novel ratiometric fluorescent signal transduction in this work. This study builds on our original discovery of a template-transformation phenomenon, which demonstrated that the connection of a special DNA fragment (5'-CACCGCTTT-3') with a green-emitting AgNC nucleation sequence (GNuS, 5'-TGCCTTTTGGGGACGGATA-3') creates a red-emitting AgNC nucleation sequence (RNuS, 5'-CACCGCTTTTGCCTTTTGGGGACGGATA-3')...
April 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28285202/a-novel-silver-nanocluster-in-situ-synthesized-as-versatile-probe-for-electrochemiluminescence-and-electrochemical-detection-of-thrombin-by-multiple-signal-amplification-strategy
#16
Guifen Jie, Lu Tan, Yang Zhao, Xiaochun Wang
In this work, a novel silver nanoclusters (AgNCs) were in situ synthesized and used as versatile electrochemiluminescence (ECL) and electrochemical (EL) signal probes for thrombin detection by using DNAzyme-assisted target recycling and hybridization chain reaction (HCR) multiple amplification strategy. The presence of target thrombin firstly opened the hairpin DNA, followed by DNAzyme-catalytic recycling cleavage of excess substrates, which could generate large number of substrate fragments (s1). Then these s1 fragments were captured by SH-DNA on the Au nanoparticle-modified electrode, which further triggered the subsequent HCR of the hairpin DNA probes (H1 and H2) to form the long dsDNA...
March 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28262705/a-selective-and-label-free-strategy-for-rapid-screening-of-telomere-binding-ligands-via-fluorescence-regulation-of-dna-silver-nanocluster
#17
Rui Cheng, Jing Xu, Xiafei Zhang, Zhilu Shi, Qi Zhang, Yan Jin
Herein, the conformational switch of G-rich oligonucleotide (GDNA) demonstrated the obvious functional switch of GDNA which was found to significantly affect the fluorescence of the in-situ synthesized DNA/silver nanocluster (DNA-AgNC) in homogeneous solution. We envisioned that the allosteric interaction between GDNA and DNA-AgNC would be possible to be used for screening telomere-binding ligands. A unimolecular probe (12C5TG) is ingeniously designed consisting of three contiguous DNA elements: G-rich telomeric DNA (GDNA) as molecular recognition sequence, T-rich DNA as linker and C-rich DNA as template of DNA-AgNC...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28194950/pinpoint-the-positions-of-single-nucleotide-polymorphisms-by-a-nanocluster-dimer
#18
Jie Liu, Yuexiang Lu, Lu Feng, Song Wang, Shixi Zhang, Xuewei Zhu, Linfeng Sheng, Sichun Zhang, Xinrong Zhang
Single nucleotide polymorphisms (SNPs) are the most fundamental internal causes for many genetic diseases. However, the location information on SNPs in a specific DNA sequence is not well acquired through current SNPs detection methods, except for accurate DNA sequencing. Here we report a fluorescence enhancement phenomenon in the process of two silver nanoclusters (AgNCs) approaching closely to form a nanocluster dimer (NCD). The fluorescence intensity is sensitive to the distance between two AgNCs; therefore, the NCD lights into different fluorescence intensities upon binding SNPs targets with mismatched bases at different positions...
February 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28140587/probing-the-absorption-and-emission-transition-dipole-moment-of-dna-stabilized-silver-nanoclusters
#19
Emma N Hooley, Miguel R Carro-Temboury, Tom Vosch
Using single molecule polarization measurements, we investigate the excitation and emission polarization characteristics of DNA stabilized silver nanoclusters (C24-AgNCs). Although small changes in the polarization generally accompany changes to the emission spectrum, the emission and excitation transition dipoles tend to be steady over time and aligned in a similar direction, when immobilized in PVA. The emission transition dipole patterns, observed for C24-AgNCs in defocused wide field imaging, match that of a single emitter...
February 9, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28139780/specific-and-sensitive-detection-of-plasmodium-falciparum-lactate-dehydrogenase-by-dna-scaffolded-silver-nanoclusters-combined-with-an-aptamer
#20
Wei-Xian Wang, Yee-Wai Cheung, Roderick M Dirkzwager, Wai-Chung Wong, Julian A Tanner, Hong-Wei Li, Yuqing Wu
Innovative nanomaterials offer significant potential for diagnosis of severe diseases of the developing world such as malaria. Small sized silver nanoclusters have shown promise for diagnostics due to their intense fluorescence emission and photo-stabilities. Here, double-stranded DNA-scaffolded silver nanoclusters (AgNCs-dsDNA) were prepared to detect the established malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). Significant luminescence enhancement over a wide concentration range of PfLDH was demonstrated...
January 31, 2017: Analyst
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