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DNA silver nanocluster

Bi-Yun Fang, Cheng Li, Jie An, Sun-Duo Zhao, Zhi-Yong Zhuang, Yuan-Di Zhao, Yue-Xuan Zhang
In this paper, DNA containing six cytosines as the formation site for silver nanoclusters (Ag NCs) was adopted as a template for preparing fluorescent DNA-Ag NCs. For the first time, it was found that the fluorescence of DNA-Ag NCs could be quenched after hybridization with their complementary sequence. On the basis of this new phenomenon, we designed a sequence C1 that was completely complementary to human immunodeficiency virus (HIV) DNA, and probe DNA which was partially complementary to C1 for the synthesis of DNA-Ag NCs...
March 7, 2018: Nanoscale
Baozhu Zhang, Chunying Wei
A novel turn-on fluorescent biosensor has been constructed using C-PS2.M-DNA-templated silver nanoclusters (Ag NCs) with an average diameter of about 1 nm. The proposed approach presents a low-toxic, simple, sensitive, and selective detection for Pb2+ . The fluorescence intensity of C-PS2.M-DNA-Ag NCs enhances significantly in the presence of Pb2+ , which is attributed to the special interaction between Pb2+ and its aptamer DNA PS2.M. Pb2+ induces the aptamer to form G-quadruplex and makes two darkish DNA/Ag NCs located at the 3' and 5' terminus close, resulting in the fluorescence light-up...
May 15, 2018: Talanta
Taotao Li, Huan Yi, Yuan Liu, Zunliang Wang, Shiquan Liu, Nongyue He, Hongna Liu, Yan Deng
DNA strands have been used as templates to form different sized silver nanoclusters. Although DNA templated silver nanoclusters (NCs) probes which show outstanding fluorescence properties have been widely applied in chemical sensing and cellular imaging, synthesis of DNA template Au-Ag bimetallic nanoclusters remains a challenge. A facile one-step synthesis method was thus developed in this study to form Au-Ag NCs using C4-ATAT-C4 as template. C4-ATAT-C4 acted as a stabilizer for preventing aggregation of the Au-Ag NCs...
January 1, 2018: Journal of Biomedical Nanotechnology
Shi Ting Lee, David Beaumont, Xiao Di Su, Kasturi Muthoosamy, Siu Yee New
Single strand DNA (ssDNA) chimeras consisting of a silver nanoclusters-nucleating sequence (NC) and an aptamer are widely employed to synthesize functional silver nanoclusters (AgNCs) for sensing purpose. Despite its simplicity, this chimeric-templated AgNCs often leads to undesirable turn-off effect, which may suffer from false positive signals caused by interference. In our effort to elucidate how the relative position of NC and aptamer affects the fluorescence behavior and sensing performance, we systematically formulated these NC and aptamer regions at different position in a DNA chimera...
June 20, 2018: Analytica Chimica Acta
Fei Qu, Zeqiu Chen, Jinmao You, Cuihua Song
Human telomere DNA plays a vital role in genome integrity control and carcinogenesis as an indication for extensive cell proliferation. Herein, silver nanoclusters (Ag NCs) templated by polymer and unmodified gold nanoparticles (Au NPs) are designed as a new colorimetric platform for sensitively differentiating telomere DNA with different lengths, monitoring G-quadruplex and dsDNA. Ag NCs can produce the aggregation of Au NPs, so the color of Au NPs changes to blue and the absorption peak moves to 700nm. While the telomere DNA can protect Au NPs from aggregation, the color turns to red again and the absorption band blue shift...
February 7, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Dan Han, Chunying Wei
In this work, we develop a fluorescent molecular beacon based on the DNA-templated silver nanoclusters (DNA-Ag NCs). The skillfully designed molecular beacon can be conveniently used for detection of diverse virulence genes as long as the corresponding recognition sequences are embedded. Importantly, the constructed detection system allows simultaneous detection of multiple nucleic acids, which is attributed to non-overlapping emission spectra of the as-synthesized silver nanoclusters. Based on the target-induced fluorescence enhancement, three infectious disease-related genes HIV, H1N1, and H5N1 are detected, and the corresponding detection limits are 3...
May 1, 2018: Talanta
Sidsel Ammitzbøll Bogh, Miguel R Carro-Temboury, Cecilia Cerretani, Steven M Swasey, Stacy M Copp, Elisabeth G Gwinn, Tom Vosch
In this paper we present a new near-IR emitting silver nanocluster (NIR-DNA-AgNC) with an unusually large Stokes shift between absorption and emission maximum (211 nm or 5600 cm-1). We studied the effect of viscosity and temperature on the steady state and time-resolved emission. The time-resolved results on NIR-DNA-AgNC show that the relaxation dynamics slow down significantly with increasing viscosity of the solvent. In high viscosity solution, the spectral relaxation stretches well into the nanosecond scale...
February 9, 2018: Methods and Applications in Fluorescence
Yasaman-Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali, Saman Hosseinkhani
BRCA1 (breast cancer 1) genomic deletions are the most important founder mutations in breast cancer patients and can be passed to you from your mother or father. Herein, we report a silver nanoclusters-based (AgNCs-based) fluorescence resonance energy transfer (FRET) method for detection of BRCA1 gene deletion. The method relies on the specific hybridization of DNA-AgNCs fluorescent probe to deleted genes and interaction between double stranded DNA-AgNCs and QD, and the signal amplification through energy transfer from fluorescent AgNCs to QDs during FRET...
January 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Duo Li, Zhenzhen Qiao, Yanru Yu, Jinlu Tang, Xiaoxiao He, Hui Shi, Xiaosheng Ye, Yanli Lei, Kemin Wang
A facile, general and turn-on nucleus imaging strategy was first developed based on in situ fluorescence activation of C-rich dark silver nanoclusters by G-rich telomeres. After a simple incubation without washing, nanoclusters could selectively stain the nucleus with intense red luminescence, which was confirmed using fixed/living cells and several cell lines.
January 12, 2018: Chemical Communications: Chem Comm
Xiaoru Zhang, Fengting Chen, Xiaojie Song, Peng He, Shusheng Zhang
A novel method for study the interaction between carbohydrate and lectin was developed in homogenous solution. DNA was introduced to the recognition process of carbohydrate with protein. Due to the versatile detection mode of DNA, the detection efficiency for carbohydrate and protein was highly promoted. Herein, two kinds of mannose-DNA conjugates were synthesized. One of which was used to prepare DNA-templated silver nanocluster (AgNCs) and the other one contained guanine-rich DNA sequences. When the mannose of two conjugates binding to lectin Concanavalin A (Con A), great enhancement on fluorescence intensity was obtained due to the proximity ligation of the DNA-templated Ag NCs with guanine-rich DNA sequences...
December 31, 2017: Biosensors & Bioelectronics
Jinhui Geng, Chi Yao, Xiaohong Kou, Jianpu Tang, Dan Luo, Dayong Yang
DNA has arisen as a promising building material for the construction of hydrogels owing to its unique properties such as designability and biocompatibility. All-DNA hydrogels with only DNA molecules may have limited applications; hence a composite DNA hydrogel with multifunctional moieties is highly desired to cater for specific applications. Herein, a multifunctional DNA hydrogel is created by incorporating DNA with silver nanoclusters (AgNCs), in which AgNCs render the hydrogel simultaneously with fluorescent and antibacterial functions...
December 27, 2017: Advanced Healthcare Materials
Xiaohong Zhang, Yunxia Qian, Xuejuan Ma, Mengfan Xia, Shuangqin Li, Yaodong Zhang
Thiolated DNA (DNA-SH) was employed as a template in the synthesis and stabilization of AgNCs (DNA-SH-AgNCs). Resulting from the synergistic protective effect of specific Ag+ -DNA interactions and Ag-S bonding, DNA-SH-AgNCs exhibited high quantum yields and resistance to oxidation.
December 21, 2017: Nanoscale
Zhikun Zhang, Yang Liu, Peifeng Liu, Lu Yang, Xingyu Jiang, Dan Luo, Dayong Yang
Chirality plays essential roles in life systems such that l-amino acids (LAAs) are predominantly found as the building units of protein for organisms. The presence of the d-enantiomer (DAA) has been found to be specifically relevant to gastric cancer. We herein construct a luminescent DNA/silver nanocluster based biosensing system to achieve rapid and specific detection of DAAs. As a proof of application, we detected DAAs in saliva samples from patients with gastric cancer, and the test results exhibited excellent specificity...
December 14, 2017: Nanoscale
Ivan L Volkov, Zakhar V Reveguk, Pavel Yu Serdobintsev, Ruslan R Ramazanov, Alexei I Kononov
The ordered structure of UV chromophores in DNA resembles photosynthetic light-harvesting complexes in which quantum coherence effects play a major role in highly efficient directional energy transfer. The possible role of coherent excitons in energy transport in DNA remains debated. Meanwhile, energy transport properties are greatly important for understanding the mechanisms of photochemical reactions in cellular DNA and for DNA-based artificial nanostructures. Here, we studied energy transfer in DNA complexes formed with silver nanoclusters and with intercalating dye (acridine orange)...
November 23, 2017: Nucleic Acids Research
Cecilia Cerretani, Miguel R Carro-Temboury, Stefan Krause, Sidsel Ammitzbøll Bogh, Tom Vosch
The nanosecond excited state temporal and spectral relaxation of a purified, red-emitting DNA-templated silver nanocluster (DNA-AgNC) was characterized as a function of temperature. The findings are explained by introducing a phenomenological electronic structure diagram. The reproducibility and cyclability of the average decay time opens up the possibility of using DNA-AgNCs for decay time-based nanothermometry.
November 8, 2017: Chemical Communications: Chem Comm
Yanjun Ding, Xingmei Li, Yadong Guo, Jie Yan, Jiang Ling, Weichen Li, Lingmei Lan, Yunfeng Chang, Jifeng Cai, Lagabaiyla Zha
In recent years, drug abuse has been considered as a most challenging social problem that aroused public attention. Ketamine has increased in unregulated use as a 'recreational drug' in teenagers. However, there is no suitable and maneuverable detection method for ketamine in situ at the moment. Fluorescence sensor technique, with predominant recognition and simple operation, is a good potential application in drug detection. Here, we first reported a highly sensitive and selective fluorescence genosensor for rapid detection of ketamine based on DNA-templated silver nanoclusters (DNA-AgNCs) probes, in which the DNA sequence could specially recognize ketamine with high affinity...
December 2017: Analytical and Bioanalytical Chemistry
Yasaman Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali
Here we describe a label-free detection strategy for large deletion mutation in breast cancer related gene BRCA1 based on a DNA-silver Nanocluster Fluorescence upon Recognition-Induced Hybridization. The specific hybridization of DNA templated silver nanoclusters fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs Fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescent intensities. Under the optimal conditions, the fluorescent intensity of the DNA-AgNCs increased with the increasing deletion type within a dynamic range from 1...
August 31, 2017: Methods and Applications in Fluorescence
Yanjun Ding, Xingmei Li, Ceng Chen, Jiang Ling, Weichen Li, Yadong Guo, Jie Yan, Lagabaiyla Zha, Jifeng Cai
Hydrogen sulfide (H2S) is a highly toxic gas as a cause of inhalational death. Accurate detection of H2S poisoning concentration is valuable and vital for forensic workers to estimate the cause of death. But so far, it is no uniform and reliable standard method to measure sulfide concentrations in H2S poisoning blood for forensic identification. This study introduces a fluorescence sensing technique into forensic research, in which a DNA-templated copper/silver nanocluster (DNA-Cu/AgNCs) fluorescence probe has been proposed to selective detection of S(2-)...
August 29, 2017: Scientific Reports
Zhenjun Liu, Wansong Chen, Yajin Han, Jiang Ouyang, Min Chen, Shengqiang Hu, Liu Deng, You-Nian Liu
Recently, membrane proteins have been considered as candidate cancer biomarkers and drug targets, due to their important roles in numerous physiological processes. Therefore, a facile, sensitive and quantitative detection of the membrane proteins is crucial for better understanding their roles in cancer cells and further validating their function in clinical research. We report a highly facile and sensitive detection method for membrane proteins on living cells in situ based on membrane protein-triggered release of cytosine (C)-rich single-stranded DNA (ssDNA) sequences, and the subsequent silver nanoclusters (AgNCs) transfer from polymer to C-rich ssDNA...
December 1, 2017: Talanta
Katarzyna Brach, Magdalena Waszkielewicz, Joanna Olesiak-Banska, Marek Samoc, Katarzyna Matczyszyn
We report on two-photon excitation properties of small silver-doped gold nanoclusters (AuAgNCs) and on their three-dimensional arrangement in a hybrid system composed of DNA liquid crystals (LCs) and AuAgNCs. UV-vis and fluorescence spectroscopy, transmission electron microscopy (TEM), and multiphoton excitation spectroscopy were used to characterize the nanoparticles. We show that AuAgNCs exhibit two-photon excited luminescence (2PL) emission and second-harmonic generation (SHG) and that these properties remain the same in liquid crystalline matrix...
August 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
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