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https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#1
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
May 16, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28491245/frequency-evaluation-of-genes-encoding-siderophores-and-the-effects-of-different-concentrations-of-fe-ions-on-growth-rate-of-uropathogenic-escherichia-coli
#2
Behnoush Khasheii, Shaghayegh Anvari, Ailar Jamalli
BACKGROUND AND OBJECTIVES: Bacteria need iron for growth and most of them can actively acquire Fe ions using especial iron-chelating proteins which named siderophores. We aimed to determine the frequencies of iucA, iroN and irp2 genes in the uropathogenic Escherichia coli (UPEC) isolates. We also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. MATERIALS AND METHODS: Totally, 170 E. coli strains were isolated from urinary tract infections and the presence of 3 siderophore genes were analyzed using PCR among them...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28489862/novel-genes-associated-with-enhanced-motility-of-escherichia-coli-st131
#3
Asha Kakkanat, Minh-Duy Phan, Alvin W Lo, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is the cause of ~75% of all urinary tract infections (UTIs) and is increasingly associated with multidrug resistance. This includes UPEC strains from the recently emerged and globally disseminated sequence type 131 (ST131), which is now the dominant fluoroquinolone-resistant UPEC clone worldwide. Most ST131 strains are motile and produce H4-type flagella. Here, we applied a combination of saturated Tn5 mutagenesis and transposon directed insertion site sequencing (TraDIS) as a high throughput genetic screen and identified 30 genes associated with enhanced motility of the reference ST131 strain EC958...
2017: PloS One
https://www.readbyqxmd.com/read/28485709/occurrence-and-characterization-of-class-1-integrons-in-escherichia-coli-from-healthy-individuals-and-those-with-urinary-infection
#4
Clarisse Oliveira-Pinto, Cristiane Diamantino, Patrícia L Oliveira, Mariana P Reis, Patrícia S Costa, Magna C Paiva, Regina M D Nardi, Paula P Magalhães, Edmar Chartone-Souza, Andréa M A Nascimento
PURPOSE: Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40). METHODOLOGY: The methods used included PCR, sequencing and antimicrobial susceptibility testing...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28478807/aqueous-extract-from-orthosiphon-stamineus-leaves-prevents-bladder-and-kidney-infection-in-mice
#5
S Sarshar, S Brandt, M R Asadi Karam, M Habibi, S Bouzari, M Lechtenberg, U Dobrindt, X Qin, F M Goycoolea, A Hensel
BACKGROUND: Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. PURPOSES: Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. STUDY DESIGN: In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing...
May 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28468965/cell-specific-qrt-pcr-of-renal-epithelial-cells-reveals-a-novel-innate-immune-signature-in-murine-collecting-duct
#6
Vijay Saxena, David S Hains, John Ketz, Melinda Chanley, John D Spencer, Brian Becknell, Keith R Pierce, Raoul D Nelson, Jeffrey M Purkerson, George J Schwartz, Andrew L Schwaderer
The urinary tract is usually culture negative despite its close proximity to microbial flora. The precise mechanisms by which the kidneys and urinary tract defends against infection is not well understood. The initial kidney cells to encounter ascending pathogens are the collecting tubule cells which consist of principal cells (PCs) that express aquaporin 2 (AQP2) and intercalated cells (ICs) that express vacuolar H+-ATPase (V-ATPase, B1 subunit). We have previously shown that ICs are involved with the human renal innate immune defense...
May 3, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28438119/dfi-seq-identification-of-environment-specific-gene-expression-in-uropathogenic-escherichia-coli
#7
Michelle Madelung, Tina Kronborg, Thomas Koed Doktor, Carsten Struve, Karen Angeliki Krogfelt, Jakob Møller-Jensen
BACKGROUND: During infection of the urinary tract, uropathogenic Escherichia coli (UPEC) are exposed to different environments, such as human urine and the intracellular environments of bladder epithelial cells. Each environment elicits a distinct bacterial environment-specific transcriptional response. We combined differential fluorescence induction (DFI) with next-generation sequencing, collectively termed DFI-seq, to identify differentially expressed genes in UPEC strain UTI89 during growth in human urine and bladder cells...
April 24, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28419868/ciprofloxacin-resistance-in-uropathogenic-escherichia-coli-isolates-causing-community-acquired-urinary-infections-in-bras%C3%A3-lia-brazil
#8
Rafaella Christina Rocha Moreira da Silva, Paulo de Oliveira Martins Júnior, Laura Fernandes Gonçalves, Vicente de Paulo Martins, Ana Beatriz Fabrício de Melo, André Pitondo-Silva, Tatiana Amabile de Campos
OBJECTIVES: Considering the global concern of ciprofloxacin resistance, the aim of this study was to evaluate the characteristics of ciprofloxacin-resistant (CIP-R) Escherichia coli isolated from patients with community-acquired urinary tract infections (UTIs) in Brasília, Brazil. METHODS: CIP-R E. coli isolated from different outpatients between July 2013 and April 2014 in a tertiary hospital were analysed for antibiotic resistance profile, phylotype, uropathogenic E...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28396353/the-histidine-residue-of-qsec-is-required-for-canonical-signaling-between-qseb-and-pmrb-in-uropathogenic-escherichia-coli
#9
Erin J Breland, Ellisa W Zhang, Tomas Bermudez, Charles R Martinez, Maria Hadjifrangiskou
Two-component systems are prototypically comprised of a histidine kinase (sensor) and a response regulator (responder). The sensor kinases auto-phosphorylate at a conserved histidine residue, acting as a phospho-donor for subsequent phosphotransfer to and activation of a cognate response regulator. In rare cases, the histidine residue is also essential for response regulator de-phosphorylation via a reverse-phosphotransfer reaction. In this work, we present an example of a kinase that relies on reverse-phosphotransfer to catalyze the de-phosphorylation of its cognate partner...
April 10, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28359738/development-of-a-multiplex-real-time-pcr-assay-for-phylogenetic-analysis-of-uropathogenic-escherichia-coli
#10
Mojtaba Hasanpour, Akram Najafi
Uropathogenic Escherichia coli (UPEC) is among major pathogens causing 80-90% of all episodes of urinary tract infections (UTIs). Recently, E. coli strains are divided into eight main phylogenetic groups including A, B1, B2, C, D, E, F, and clade I. This study was aimed to develop a rapid, sensitive, and specific multiplex real time PCR method capable of detecting phylogenetic groups of E. coli strains. This study was carried out on E. coli strains (isolated from the patient with UTI) in which the presence of all seven target genes had been confirmed in our previous phylogenetic study...
March 28, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28330863/bacterial-virulence-phenotypes-of-escherichia-coli-and-host-susceptibility-determine-risk-for-urinary-tract-infections
#11
Henry L Schreiber, Matt S Conover, Wen-Chi Chou, Michael E Hibbing, Abigail L Manson, Karen W Dodson, Thomas J Hannan, Pacita L Roberts, Ann E Stapleton, Thomas M Hooton, Jonathan Livny, Ashlee M Earl, Scott J Hultgren
Urinary tract infections (UTIs) are caused by uropathogenic Escherichia coli (UPEC) strains. In contrast to many enteric E. coli pathogroups, no genetic signature has been identified for UPEC strains. We conducted a high-resolution comparative genomic study using E. coli isolates collected from the urine of women suffering from frequent recurrent UTIs. These isolates were genetically diverse and varied in their urovirulence, that is, their ability to infect the bladder in a mouse model of cystitis. We found no set of genes, including previously defined putative urovirulence factors (PUFs), that were predictive of urovirulence...
March 22, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28302076/uropathogenic-escherichia-coli-pathogenicity-islands-and-other-expec-virulence-genes-may-contribute-to-the-genome-variability-of-enteroinvasive-e-coli
#12
Laís Cristina da Silva, Ana Carolina de Mello Santos, Rosa Maria Silva
BACKGROUND: Enteroinvasive Escherichia coli (EIEC) may be the causative agent of part of those million cases of diarrhea illness reported worldwide every year and attributable to Shigella. That is because both enteropathogens have many common characteristics that difficult their identification either by traditional microbiological methods or by molecular tools used in the clinical laboratory settings. While Shigella has been extensively studied, EIEC remains barely characterized at the molecular level...
March 16, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28280491/comparative-analysis-of-phylogenetic-assignment-of-human-and-avian-expec-and-fecal-commensal-escherichia-coli-using-the-previous-and-revised-clermont-phylogenetic-typing-methods-and-its-impact-on-avian-pathogenic-escherichia-coli-apec-classification
#13
Catherine M Logue, Yvonne Wannemuehler, Bryon A Nicholson, Curt Doetkott, Nicolle L Barbieri, Lisa K Nolan
The Clermont scheme has been used for subtyping of Escherichia coli since it was initially described in early 2000. Since then, researchers have used the scheme to type and sub-type commensal E. coli and pathogenic E. coli, such as extraintestinal pathogenic E. coli (ExPEC), and compare their phylogenetic assignment by pathogenicity, serogroup, distribution among ExPEC of different host species and complement of virulence and resistance traits. Here, we compare assignments of human and avian ExPEC and commensal E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28270808/transcriptional-control-of-dual-transporters-involved-in-%C3%AE-ketoglutarate-utilization-reveals-their-distinct-roles-in-uropathogenic-escherichia-coli
#14
Wentong Cai, Xuwang Cai, Yongwu Yang, Shigan Yan, Haibin Zhang
Uropathogenic Escherichia coli (UPEC) are the primary causative agents of urinary tract infections. Some UPEC isolates are able to infect renal proximal tubule cells, and can potentially cause pyelonephritis. We have previously shown that to fulfill their physiological roles renal proximal tubule cells accumulate high concentrations of α-ketoglutarate (KG) and that gene cluster c5032-c5039 contribute to anaerobic utilization of KG by UPEC str. CFT073, thereby promoting its in vivo fitness. Given the importance of utilizing KG for UPEC, this study is designed to investigate the roles of two transporters KgtP and C5038 in KG utilization, their transcriptional regulation, and their contributions to UPEC fitness in vivo...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28262045/recurrent-urinary-tract-infections-in-women-risk-factors-etiology-pathogenesis-and-prophylaxis
#15
Antonio Guglietta
Urinary tract infections (UTIs) are one of the most common bacterial infections in women, often as a recurrent disease. Uropathogenic Escherichia coli (UPEC) is the most common pathotype of extraintestinal pathogenic E. coli (ExPEC) found among patients with UTI. The human intestinal can act as a reservoir of UPEC, with the female urethra being infected by fecal material containing UPEC. Adhesion of bacteria to the epithelial cells of urogenital mucosa is an important mechanism in the pathogenesis of UTI. Alternative nonantibiotic based approaches, such as mechanical barrier protection of the intestinal mucosa have been proposed to reduce bacterial adherence to intestinal epithelium, bacteria proliferation and decrease of the load of UPEC in the intestinal lumen and in the fecal material...
March 2017: Future Microbiology
https://www.readbyqxmd.com/read/28228557/surfactant-protein-a-inhibits-growth-and-adherence-of-uropathogenic-escherichia-coli-to-protect-the-bladder-from-infection
#16
Jiro Hashimoto, Motoko Takahashi, Atsushi Saito, Masaki Murata, Yuichiro Kurimura, Chiaki Nishitani, Rina Takamiya, Yasuaki Uehara, Yoshihiro Hasegawa, Yoshiki Hiyama, Norimasa Sawada, Satoshi Takahashi, Naoya Masumori, Yoshio Kuroki, Shigeru Ariki
Surfactant protein A (SP-A) is a multifunctional host defense collectin that was first identified as a component of pulmonary surfactant. Although SP-A is also expressed in various tissues, including the urinary tract, its innate immune functions in nonpulmonary tissues are poorly understood. In this study, we demonstrated that adherence of uropathogenic Escherichia coli (UPEC) to the bladder was enhanced in SP-A-deficient mice, which suggests that SP-A plays an important role in innate immunity against UPEC...
February 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28217700/the-multiple-antibacterial-activities-of-the-bladder-epithelium
#17
REVIEW
Jianxuan Wu, Yuxuan Miao, Soman N Abraham
The urinary tract is subject to frequent challenges from the gut microflora. Indeed, up to 40% of women will experience at least one urinary tract infection (UTI) during their lifetime. Uropathogenic Escherichia coli (UPEC) contribute to an overwhelming majority of these cases and they typically initiate UTIs by invading the superficial epithelium that lines the bladder lumen. In addition to serving as an effective barrier to noxious agents found in urine, bladder epithelial cells (BECs) play a key physiological role in regulating bladder volume to accommodate urine flow...
January 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28217123/virulence-markers-and-phylogenetic-analysis-of-escherichia-coli-strains-with-hybrid-eaec-upec-genotypes-recovered-from-sporadic-cases-of-extraintestinal-infections
#18
Flaviane B M Lara, Danielly R Nery, Pâmela M de Oliveira, Mayana L Araujo, Fabiana R Q Carvalho, Lorena C F Messias-Silva, Leonardo B Ferreira, Celio Faria-Junior, Alex L Pereira
Virulence genes from different E. coli pathotypes are blended in hybrid strains. E. coli strains with hybrid enteroaggregative/uropathogenic (EAEC/UPEC) genotypes have sporadically emerged causing outbreaks of extraintestinal infections, however their association with routine infections is yet underappreciated. We assessed 258 isolates of E. coli recovered from 86 consecutive cases of extraintestinal infections seeking EAEC and hybrid genotype (EAEC/UPEC) strains. Extensive virulence genotyping was carried out to detect 21 virulence genes, including molecular predictors of EAEC and UPEC strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28208853/genotypic-characterization-of-virulence-factors-in-escherichia-coli-isolated-from-patients-with-acute-cystitis-pyelonephritis-and-asymptomatic-bacteriuria
#19
Mohsen Tabasi, Mohammad Reza Asadi Karam, Mehri Habibi, Ehsan Mostafavi, Saeid Bouzari
INTRODUCTION: Urinary Tract Infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) are among the most common infections worldwide. It is well-documented that the pathogenesis of UPEC is mediated by the production of a wide variety of Virulence Factors (VFs). Thus, detection of these VFs and evaluation of their association with different clinical types of UTIs could help to understand the role of these factors in pathogenesis of UPEC isolates. AIM: To investigate the genotypic characteristics of UPEC isolates and to examine the relationship between VFs and different clinical symptoms of UTI...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28142108/homeopathic-medicine-cantharis-modulates-uropathogenic-e-coli-upec-induced-cystitis-in-susceptible-mice
#20
Cidéli de Paula Coelho, Priscilla Dias Motta, Mariana Petrillo, Renata de Oliveira Iovine, Luciane Costa Dalboni, Fabiana Rodrigues Santana, Michelle Sanchez Freitas Correia, Renato Corrêa Viana Casarin, Vania Maria Carvalho, Leoni Villano Bonamin
OBJECTIVE: This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coli-induced cystitis. METHODS: 24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC O4:K-:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT...
January 28, 2017: Cytokine
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