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https://www.readbyqxmd.com/read/29327292/alterations-of-growth-rate-and-gene-expression-levels-of-upec-by-antibiotics-at-sub-mic
#1
Defne Gümüş, Fatma Kalaycı-Yüksek, Emre Yörük, Gülşen Uz, Eşref Çelik, Cansu Arslan, Elif Merve Aydın, Cem Canlı, Mine Anğ-Küçüker
The host is the main environment for bacteria, and they also expose to many antibiotics during the treatment of infectious diseases in host body. In this study, it was aimed to investigate possible changes in growth rate and expression levels of three virulence genes (foc/foc, cnf1, and usp) in a uropathogenic E. coli standard strain within the presence of ciprofloxacin, nitrofurantoin, and trimethoprim-sulfamethoxazole. The UPEC C7 strain was grown on tryptic soy broth-TSB (control), TSB + ciprofloxacin, TSB + nitrofurantoin, and TSB + trimethoprim-sulfamethoxazole for determination of both growth rate and gene expression level...
January 11, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29326207/complete-genome-sequence-of-bacteriophage-st11ph5-which-infects-uropathogenic-escherichia-coli-strain-up11
#2
Alla K Golomidova, Eugene E Kulikov, Vladislav V Babenko, Elena S Kostryukova, Andrey V Letarov
Bacteriophage St11Ph5 was isolated from a sewage sample on a particularly phage-resistant uropathogenic Escherichia coli (UPEC) up11 host strain. It appeared to be closely related to bacteriophage G7C, isolated from horse feces; however, it carries a highly divergent host recognition module.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29324795/nanodiamonds-facilitate-killing-of-intracellular-uropathogenic-e-coli-in-an-in-vitro-model-of-urinary-tract-infection-pathogenesis
#3
Janaki Kannan Iyer, Alexia Dickey, Parvaneh Rouhani, Anil Kaul, Nirmal Govindaraju, Raj Narain Singh, Rashmi Kaul
About 25-44% of women will experience at least one episode of recurrent UTI and the causative agent in over 70% of UTI cases is uropathogenic Escherichia coli (UPEC). UPEC cause recurrent UTI by evading the bladder's innate immune system through internalization into the bladder epithelium where antibiotics cannot reach or be effective. Thus, it is important to develop novel therapeutics to eliminate these intracellular pathogens. Nanodiamonds (NDs) are biocompatible nanomaterials that serve as promising candidates for targeted therapeutic applications...
2018: PloS One
https://www.readbyqxmd.com/read/29311237/evaluation-of-cpxra-as-a-therapeutic-target-for-uropathogenic-escherichia-coli-infections
#4
Lana Dbeibo, Julia J van Rensburg, Sara N Smith, Kate R Fortney, Dharanesh Gangaiah, Hongyu Gao, Juan Marzoa, Yunlong Liu, Harry L T Mobley, Stanley M Spinola
CpxRA is an envelope stress response system found in all species of Enterobacteriaceae; CpxA has kinase activity for CpxR and phosphatase activity for phospho-CpxR, a transcription factor. CpxR also accepts phosphate groups and acetyl phosphate, a glucose metabolite. Activation of CpxR increases transcription of genes encoding for membrane repair and downregulates virulence determinants. We hypothesized that activation of CpxR could serve as an antimicrobial/antivirulence strategy and discovered compounds that activate CpxR in Escherichia coli by inhibiting CpxA phosphatase activity...
January 8, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29310594/the-impact-of-cathelicidin-the-human-antimicrobial-peptide-ll-37-in-urinary-tract-infections
#5
Ibrahim H Babikir, Elsir A Abugroun, Naser Eldin Bilal, Abdullah Ali Alghasham, Elmuataz Elmansi Abdalla, Ishag Adam
BACKGROUND: The defense mechanisms of the urinary tract are attributed mainly to the innate immune system and the urinary tract urothelium which represent the first line of defense against invading pathogens and maintaining sterility of the urinary tract. There are only a few publications regarding cathelicidin (LL-37) and a urinary tract infection (UTI). This study was done to investigate the plasma and urine levels of human LL-37 in patients with UTI. METHODS: A case-control study was conducted at Omdurman Hospital, Sudan during the period from August 2014 to May 2017...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29300775/analytical-profiling-of-mutations-in-quinolone-resistance-determining-region-of-gyra-gene-among-upec
#6
Lesley R Varughese, Meenakshi Rajpoot, Soniya Goyal, Ravinder Mehra, Vinod Chhokar, Vikas Beniwal
Mutations in gyrA are the primary cause of quinolone resistance encountered in gram-negative clinical isolates. The prospect of this work was to analyze the role of gyrA mutations in eliciting high quinolone resistance in uropathogenic E.coli (UPEC) through molecular docking studies. Quinolone susceptibility testing of 18 E.coli strains isolated from UTI patients revealed unusually high resistance level to all the quinolones used; especially norfloxacin and ciprofloxacin. The QRDR of gyrA was amplified and sequenced...
2018: PloS One
https://www.readbyqxmd.com/read/29250044/clonal-diversity-virulence-potential-and-antimicrobial-resistance-of-escherichia-coli-causing-community-acquired-urinary-tract-infection-in-switzerland
#7
Magdalena T Nüesch-Inderbinen, Melinda Baschera, Katrin Zurfluh, Herbert Hächler, Hansjakob Nüesch, Roger Stephan
Objectives: The aim of this study was to assess the clonal structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli (UPEC) isolates causing community acquired urinary tract infection (CAUTI) in unselected primary care patients in Switzerland. Methods: We performed multilocus sequence typing, virulence factor determination, and phenotypic and genotypic antimicrobial resistance testing on 44 non-duplicate UPEC isolates. Results: Twenty-seven different sequence types (STs) were identified...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29247576/effect-of-non-%C3%AE-lactams-on-stable-variants-of-inhibitor-resistant-tem%C3%A2-%C3%AE-lactamase-in-uropathogenic%C3%A2-e-coli%C3%A2-implication-for-alternative-therapy
#8
Sandip Kumar Mukherjee, Rahul Shubhra Mandal, Santasabuj Das, Mandira Mukherjee
AIMS: β-lactamase inhibitor resistance(BLIR) among the uropathogenic E.coli (UPEC) minimizes treatment options. This study aims to identify inhibitor-resistant TEM (IRT) β-lactamase that impart BLIR phenotype and explore non-β-lactams as alterative therapeutics. METHODS AND RESULTS: 30 BLIR UPEC isolates was detected by Kirby-Bauer disk diffusion technique using β-lactam-β-lactamase inhibitor combination. Conjugal transfer of BLIR was successful from 17 isolates...
December 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29237285/inhibition-of-diverse-dsba-enzymes-in-multi-dsba-encoding-pathogens
#9
Makrina Totsika, Dimitrios Vagenas, Jason J Paxman, Geqing Wang, Rabeb Dhouib, Pooja Sharma, Jennifer L Martin, Martin J Scanlon, Begoña Heras
AIMS: DsbA catalyses disulfide bond formation in secreted and outer membrane proteins in bacteria. In pathogens, DsbA is a major facilitator of virulence constituting a target for antivirulence antimicrobial development. However, many pathogens encode multiple and diverse DsbA enzymes for virulence factor folding during infection. The aim of this study was to determine whether our recently identified inhibitors of <i>Escherichia coli </i>K-12 DsbA can inhibit the diverse DsbA enzymes found in two important human pathogens and attenuate their virulence...
December 14, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29229730/role-of-ethanolamine-utilization-genes-in-host-colonization-during-urinary-tract-infection
#10
Anna Sintsova, Sara Smith, Sargurunathan Subashchandrabose, Harry L Mobley
Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic E. coli (UPEC) being the major causative agent of the infection. While there has been extensive research on UPEC virulence determinants, the importance of host-specific metabolism remains understudied. Here we report that UPEC upregulate the expression of ethanolamine utilization genes during uncomplicated UTIs in humans...
December 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29199378/distribution-of-pathogenicity-island-markers-and-virulence-factors-in-new-phylogenetic-groups-of-uropathogenic-escherichia-coli-isolates
#11
Akram Najafi, Mojtaba Hasanpour, Azam Askary, Masoud Aziemzadeh, Najmeh Hashemi
The present study was aimed at investigating the relationship between the new Clermont's phylogenetic groups, virulence factors, and pathogenicity island markers (PAIs) among uropathogenic Escherichia coli (UPEC) in Iran. This cross-sectional study was carried out on 140 UPEC isolates collected from patients with urinary tract infections in Bushehr, Iran. All isolates were subjected to phylogenetic typing using a new quadruplex-PCR method. The presence of PAI markers and virulence factors in UPEC strains was evaluated by multiplex PCR...
December 3, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29198308/payload-hardware-and-experimental-protocol-development-to-enable-future-testing-of-the-effect-of-space-microgravity-on-the-resistance-to-gentamicin-of-uropathogenic-escherichia-coli-and-its-%C3%AF-s-deficient-mutant
#12
A C Matin, J-H Wang, Mimi Keyhan, Rachna Singh, Michael Benoit, Macarena P Parra, Michael R Padgen, Antonio J Ricco, Matthew Chin, Charlie R Friedericks, Tori N Chinn, Aaron Cohen, Michael B Henschke, Timothy V Snyder, Matthew P Lera, Shannon S Ross, Christina M Mayberry, Sungshin Choi, Diana T Wu, Ming X Tan, Travis D Boone, Christopher C Beasley, Matthew E Piccini, Stevan M Spremo
Human immune response is compromised and bacteria can become more antibiotic resistant in space microgravity (MG). We report that under low-shear modeled microgravity (LSMMG), stationary-phase uropathogenic Escherichia coli (UPEC) become more resistant to gentamicin (Gm), and that this increase is dependent on the presence of σs (a transcription regulator encoded by the rpoS gene). UPEC causes urinary tract infections (UTIs), reported to afflict astronauts; Gm is a standard treatment, so these findings could impact astronaut health...
November 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/29129382/the-cryoelectron-microscopy-structure-of-the-type-1-chaperone-usher-pilus-rod
#13
Manuela K Hospenthal, Dawid Zyla, Tiago R D Costa, Adam Redzej, Christoph Giese, James Lillington, Rudi Glockshuber, Gabriel Waksman
Adhesive chaperone-usher pili are long, supramolecular protein fibers displayed on the surface of many bacterial pathogens. The type 1 and P pili of uropathogenic Escherichia coli (UPEC) play important roles during urinary tract colonization, mediating attachment to the bladder and kidney, respectively. The biomechanical properties of the helical pilus rods allow them to reversibly uncoil in response to flow-induced forces, allowing UPEC to retain a foothold in the unique and hostile environment of the urinary tract...
November 7, 2017: Structure
https://www.readbyqxmd.com/read/29069656/cyclosporine-a-induces-micrornas-controlling-innate-immunity-during-renal-bacterial-infection
#14
Malick Sadio, Emilie Tourneur, Marcelle Bens, Jean-Michel Goujon, Alain Vandewalle, Cécilia Chassin
Urinary tract infections (UTIs) mainly due to uropathogenic Escherichia coli (UPEC) are one of the most frequent complications in kidney-transplanted patients, causing significant morbidity. However, the mechanisms underlying UTI in renal grafts remain poorly understood. Here, we analysed the effects of the potent immunosuppressive agent cyclosporine A (CsA) on the activation of collecting duct cells that represent a preferential site of adhesion and translocation for UPEC. CsA induced the inhibition of lipopolysaccharide- induced activation of collecting duct cells due to the downregulation of the expression of TLR4 via the microRNA Let-7i...
October 26, 2017: Journal of Innate Immunity
https://www.readbyqxmd.com/read/29066548/genome-wide-discovery-of-genes-required-for-capsule-production-by-uropathogenic-escherichia-coli
#15
Kelvin G K Goh, Minh-Duy Phan, Brian M Forde, Teik Min Chong, Wai-Fong Yin, Kok-Gan Chan, Glen C Ulett, Matthew J Sweet, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract and bloodstream infections and possesses an array of virulence factors for colonization, survival, and persistence. One such factor is the polysaccharide K capsule. Among the different K capsule types, the K1 serotype is strongly associated with UPEC infection. In this study, we completely sequenced the K1 UPEC urosepsis strain PA45B and employed a novel combination of a lytic K1 capsule-specific phage, saturated Tn5 transposon mutagenesis, and high-throughput transposon-directed insertion site sequencing (TraDIS) to identify the complement of genes required for capsule production...
October 24, 2017: MBio
https://www.readbyqxmd.com/read/29066378/the-influence-of-tissue-antioxidant-enzymes-and-inflammatory-cascade-in-pathology-of-cystitis-an-experimental-rat-model
#16
A Vysakh, D Suma, Kuriakose Jayesh, Mathew Jyothis, M S Latha
We evaluate the role of antioxidant enzyme status and inflammatory cascade in disease progression of cystitis in a rat model. The animals were injected with clinically isolated Uropathogenic Escherichia coli (UPEC) and study the effect of various antioxidant enzymes and inflammatory markers in disease pathology on the 0(th) day, 12 h and 7(th) day of infection. The antioxidant status of bladder tissue was decreased during the 7(th) day of infection. Lipid peroxidation marker MDA was increased on the 7(th) day of infection in rats...
October 21, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29038256/thiol-starvation-induces-redox-mediated-dysregulation-of-escherichia-coli-biofilm-components
#17
David A Hufnagel, Janet E Price, Rachel E Stephenson, Jesse Kelley, Matthew F Benoit, Matthew R Chapman
A hallmark of bacterial biofilms is the production of an extracellular matrix (ECM) that encases and protects the community from environmental stressors. Biofilm formation is an integral portion of the uropathogenic Escherichia coli (UPEC) lifecycle. Approximately 2% of UPEC are cysteine auxotrophs. Here, we investigated how cysteine homeostasis impacted UPEC UTI89 strain biofilm formation and, specifically, the production of the ECM components curli and cellulose. Cysteine auxotrophs produced less cellulose and slightly more curli compared to wildtype (WT) strains, and cysteine auxotrophs formed smooth, non-rugose colonies...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29035446/the-effects-of-insulin-and-glucose-on-different-characteristics-of-a-upec-alterations-in-growth-rate-and-expression-levels-of-some-virulence-genes
#18
Defne Gumus, Emre Yoruk, Fatma Kalayci-Yuksek, Gulsen Uz, Aysegul Topal-Sarikaya, Mine Ang-Kucuker
BACKGROUND: Host factors are known to modulate virulence, antibiotic susceptibility, and growth rate of bacteria. The effect of human insulin and glucose on growth rate and expression of virulence genes (usp, sfa/foc, cnf1) of a uropathogenic E. coli (UPEC) strain were investigated in this study. METHODS: E. coli C7 was grown in tryptic soy broth (TSB-control) and TSB containing 20 µU/mL insulin, 200 µU/mL insulin, 0.1% glucose, and 200 µU/mL insulin + 0.1% glucose...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28980415/carvacrol-rich-oregano-oil-and-thymol-rich-thyme-red-oil-inhibit-biofilm-formation-and-the-virulence-of-uropathogenic-escherichia-coli
#19
Jin-Hyung Lee, Yong-Guy Kim, Jintae Lee
AIMS: Urinary tract infections are caused primarily by uropathogenic Escherichia coli (UPEC), and indwelling catheters are usually colonized by UPEC biofilms tolerant to common antibiotics. Hence, UPEC biofilms pose a substantial challenge, and there is an urgent need for effective control strategies. METHODS AND RESULTS: In this study, 79 essential oils were screened for antibiofilm ability against UPEC. Components of active oils were identified, and their antibiofilm activities were also investigated using 96-well plates with crystal violet assay, scanning electron microscopy, and confocal laser scanning microscopy...
October 5, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28973965/biofilm-formation-by-uropathogenic-escherichia-coli-is-favored-under-oxygen-conditions-that-mimic-the-bladder-environment
#20
Allison R Eberly, Kyle A Floyd, Connor J Beebout, Spencer J Colling, Madison J Fitzgerald, Charles W Stratton, Jonathan E Schmitz, Maria Hadjifrangiskou
One of the most common urologic problems afflicting millions of people worldwide is urinary tract infection (UTI). The severity of UTIs ranges from asymptomatic bacteriuria to acute cystitis, and in severe cases, pyelonephritis and urosepsis. The primary cause of UTIs is uropathogenic Escherichia coli (UPEC), for which current antibiotic therapies often fail. UPEC forms multicellular communities known as biofilms on urinary catheters, as well as on and within bladder epithelial cells. Biofilm formation protects UPEC from environmental conditions, antimicrobial therapy, and the host immune system...
September 30, 2017: International Journal of Molecular Sciences
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