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https://www.readbyqxmd.com/read/29765378/epithelial-c5ar1-signaling-enhances-uropathogenic-escherichia-coli-adhesion-to-human-renal-tubular-epithelial-cells
#1
Yun Song, Kun-Yi Wu, Weiju Wu, Zhao-Yang Duan, Ya-Feng Gao, Liang-Dong Zhang, Tie Chong, Malgorzata A Garstka, Wuding Zhou, Ke Li
Recent work in a murine model of ascending urinary tract infection has suggested that C5a/C5aR1 interactions play a pathogenic role in the development of renal infection through enhancement of bacterial adhesion/colonization to renal tubular epithelial cells (RTECs). In the present study, we extended these observations to human. We show that renal tubular epithelial C5aR1 signaling is involved in promoting uropathogenic Escherichia coli (UPEC) adhesion/invasion of host cells. Stimulation of primary cultures of RTEC with C5a resulted in significant increases in UPEC adhesion/invasion of the RTEC...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29740035/antimicrobial-photodynamic-therapy-mediated-by-methylene-blue-and-potassium-iodide-to-treat-urinary-tract-infection-in-a-female-rat-model
#2
Ying-Ying Huang, Anton Wintner, Patrick C Seed, Timothy Brauns, Jeffrey A Gelfand, Michael R Hamblin
Drug-resistant urinary tract infections (UTIs) are difficult and sometimes impossible to treat. Many UTIs are caused by uropathogenic Escherichia coli (UPEC). We developed an intact rat model of UTI, by catheterizing female rats and introducing a bioluminescent UPEC strain into the female rat bladder which lasted for up to six days. We recently showed that antimicrobial photodynamic inactivation (aPDI) of a bacterial infection mediated by the well-known phenothiazinium salt, methylene blue (MB) could be strongly potentiated by addition of the non-toxic salt potassium iodide (KI)...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29683762/regulation-of-hemolysin-in-uropathogenic-escherichia-coli-fine-tunes-killing-of-human-macrophages
#3
Ambika M V Murthy, Minh-Duy Phan, Kate M Peters, Nguyen Thi Khanh Nhu, Rodney A Welch, Glen C Ulett, Mark A Schembri, Matthew J Sweet
Uropathogenic E. coli (UPEC) causes the majority of urinary tract infections (UTIs), which are a major global public health concern. UPEC uses numerous mechanisms to subvert the innate immune system, including targeting macrophage functions. We recently showed that some UPEC strains rapidly kill human macrophages via an NLRP3-independent pathway, and also trigger NLRP3-dependent IL-1β processing. In this study, we used random transposon mutagenesis in the reference strain CFT073 to identify UPEC genes that mediate human macrophage cell death...
April 23, 2018: Virulence
https://www.readbyqxmd.com/read/29662840/activation-of-the-nlrp3-inflammasome-pathway-by-uropathogenic-escherichia-coli-is-virulence-factor-dependent-and-influences-colonization-of-bladder-epithelial-cells
#4
Isak Demirel, Alexander Persson, Annelie Brauner, Eva Särndahl, Robert Kruse, Katarina Persson
The NLRP3 inflammasome and IL-1β release have recently been suggested to be important for the progression of urinary tract infection (UTI). However, much is still unknown regarding the interaction of UPEC and the NLRP3 inflammasome. The purpose of this study was to elucidate what virulence factors uropathogenic Escherichia coli (UPEC) use to modulate NLRP3 inflammasome activation and subsequent IL-1β release and the role of NLRP3 for UPEC colonization of bladder epithelial cells. The bladder epithelial cell line 5637, CRISPR/Cas9 generated NLRP3, caspase-1 and mesotrypsin deficient cell lines and transformed primary bladder epithelial cells (HBLAK) were stimulated with UPEC isolates and the non-pathogenic MG1655 strain...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29658949/urinary-tract-infections-immunity-and-vaccination
#5
Víctor Manuel Luna-Pineda, Sara Ochoa, Ariadnna Cruz-Córdova, Vicenta Cázares-Domínguez, Fernanda Vélez-González, Rigoberto Hernández-Castro, Juan Xicohtencatl-Cortes
Urinary tract infections (UTI) are considered one of the main causes of morbidity worldwide, and uropathogenic Escherichia coli (UPEC) is the etiological agent associated with these infections. The high morbidity produced by the UTI and the limitation of antibiotic treatments promotes the search for new alternatives against these infections. The knowledge that has been generated regarding the immune response in the urinary tract is important for the development of effective strategies in the UTI prevention, treatment, and control...
2018: Boletín Médico del Hospital Infantil de México
https://www.readbyqxmd.com/read/29614372/incidence-and-risk-of-co-transmission-of-plasmid-mediated-quinolone-resistance-and-extended-spectrum-%C3%AE-lactamase-genes-in-fluoroquinolone-resistant-uropathogenic-e-coli-a-first-study-from-kolkata-india
#6
Shreya Basu, Mandira Mukherjee
BACKGROUND: Co-resistance against fluroquinolone(FQ) and β-lactams cause treatment complications in infections caused by uropathogenic E.coli(UPEC).This study aims to detect coexistence and co-transmission of plasmid-mediated quinolone resistant(PMQR) and extended spectrum β-lactamases (ESBLs) in UPECs from Kolkata,India. METHODS: E. coli was detected biochemically from urine culture positive samples. Antibiotic resistance and ESBL production was confirmed by disk diffusion assay...
March 31, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29581075/distribution-of-extended-spectrum-%C3%AE-lactam-quinolone-and-carbapenem-resistance-genes-and-genetic-diversity-among-uropathogenic-escherichia-coli-isolates-in-tehran-iran
#7
Shahla Shahbazi, Mohammad Reza Asadi Karam, Mehri Habibi, Atefeh Talebi, Saeid Bouzari
OBJECTIVES: Antibiotic resistance among the Uropathogenic Escherichia coli (UPEC) is increasing. The aim of this study was to evaluate antimicrobial resistance in the UPEC isolates and their correlations with virulence factors. METHODS: A total of 120 E. coli isolates were collected from the patients with urinary tract infections in Tehran, Iran. Biofilm formation and antimicrobial susceptibilities were determined by phenotypic tests. Combination disk diffusion and modified Hodge tests (MHT) were performed for the phenotypic detection of ESBL and carbapenemase, respectively...
March 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29549670/non-histone-nuclear-protein-hmgn2-differently-regulates-the-urothelium-barrier-function-by-altering-expression-of-antimicrobial-peptides-and-tight-junction-protein-genes-in-upec-j96-infected-bladder-epithelial-cell-monolayer
#8
Hanwen Tian, Junming Miao, Fumei Zhang, Feng Xiong, Feimei Zhu, Jinyu Li, Xiaoying Wang, Shanzhe Chen, Junli Chen, Ning Huang, Yi Wang
The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection...
March 17, 2018: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29519530/antimicrobial-resistance-multilocus-sequence-types-and-virulence-profiles-of-esbl-producing-and-non-esbl-producing-uropathogenic-escherichia-coli-isolated-from-cats-and-dogs-in-switzerland
#9
Anna Lena Zogg, Katrin Zurfluh, Sarah Schmitt, Magdalena Nüesch-Inderbinen, Roger Stephan
Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n = 17/48.6% of the blaESBLs ), blaCTX-M-1 (n = 10/28.6%), blaCTX-M-55 (n = 4/11.4%), blaCTX-M-14 (n = 3/8.6%), and blaCTX-M-27 (n = 1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29511075/rapid-growth-of-uropathogenic-escherichia-coli-during-human-urinary-tract-infection
#10
Valerie S Forsyth, Chelsie E Armbruster, Sara N Smith, Ali Pirani, A Cody Springman, Matthew S Walters, Greta R Nielubowicz, Stephanie D Himpsl, Evan S Snitkin, Harry L T Mobley
Uropathogenic Escherichia coli (UPEC) strains cause most uncomplicated urinary tract infections (UTIs). These strains are a subgroup of extraintestinal pathogenic E. coli (ExPEC) strains that infect extraintestinal sites, including urinary tract, meninges, bloodstream, lungs, and surgical sites. Here, we hypothesize that UPEC isolates adapt to and grow more rapidly within the urinary tract than other E. coli isolates and survive in that niche. To date, there has not been a reliable method available to measure their growth rate in vivo Here we used two methods: segregation of nonreplicating plasmid pGTR902, and peak-to-trough ratio (PTR), a sequencing-based method that enumerates bacterial chromosomal replication forks present during cell division...
March 6, 2018: MBio
https://www.readbyqxmd.com/read/29507749/precision-antimicrobial-therapeutics-the-path-of-least-resistance
#11
REVIEW
Caitlin N Spaulding, Roger D Klein, Henry L Schreiber, James W Janetka, Scott J Hultgren
The emergence of drug-resistant pathogens has led to a decline in the efficacy of traditional antimicrobial therapy. The rise in resistance has been driven by widespread use, and in some cases misuse, of antibacterial agents in treating a variety of infections. A growing body of research has begun to elucidate the harmful effects of broad-spectrum antibiotic therapy on the beneficial host microbiota. To combat these threats, increasing effort is being directed toward the development of precision antimicrobial therapeutics that target key virulence determinants of specific pathogens while leaving the remainder of the host microbiota undisturbed...
2018: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/29507247/structure-based-discovery-of-glycomimetic-fmlh-ligands-as-inhibitors-of-bacterial-adhesion-during-urinary-tract-infection
#12
Vasilios Kalas, Michael E Hibbing, Amarendar Reddy Maddirala, Ryan Chugani, Jerome S Pinkner, Laurel K Mydock-McGrane, Matt S Conover, James W Janetka, Scott J Hultgren
Treatment of bacterial infections is becoming a serious clinical challenge due to the global dissemination of multidrug antibiotic resistance, necessitating the search for alternative treatments to disarm the virulence mechanisms underlying these infections. Uropathogenic Escherichia coli (UPEC) employs multiple chaperone-usher pathway pili tipped with adhesins with diverse receptor specificities to colonize various host tissues and habitats. For example, UPEC F9 pili specifically bind galactose or N -acetylgalactosamine epitopes on the kidney and inflamed bladder...
March 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29496563/antiadhesive-hydroalcoholic-extract-from-apium-graveolens-fruits-prevents-bladder-and-kidney-infection-against-uropathogenic-e-coli
#13
S Sarshar, J Sendker, X Qin, F M Goycoolea, M R Asadi Karam, M Habibi, S Bouzari, U Dobrindt, A Hensel
Fruits from Apium graveolens (Celery) are used traditionally in Persian and European medicine for the treatment of uncomplicated urinary tract infections. No data are available on A. graveolens extract on the interplay between uropathogenic E. coli and the eukaryotic host cells and on quorum sensing of the bacteria. The present study aimed to characterize an antiadhesive and anti quorum sensing effect of a characterized A. graveolens extract by specific in vitro assays and to correlate these effects with in vivo data obtained by an animal infection model...
February 26, 2018: Fitoterapia
https://www.readbyqxmd.com/read/29491858/characterization-of-asymptomatic-bacteriuria-escherichia-coli-isolates-in-search-of-alternative-strains-for-efficient-bacterial-interference-against-uropathogens
#14
Christoph Stork, Beáta Kovács, Barnabás Rózsai, Johannes Putze, Matthias Kiel, Ágnes Dorn, Judit Kovács, Szilvia Melegh, Andreas Leimbach, Tamás Kovács, György Schneider, Monika Kerényi, Levente Emödy, Ulrich Dobrindt
Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can prevent bladder colonization by uropathogens and thus symptomatic urinary tract infection (UTI). Deliberate bladder colonization with Escherichia coli ABU isolate 83972 has been shown to outcompete uropathogens and prevent symptomatic UTI by bacterial interference. Many ABU isolates evolved from uropathogenic ancestors and, although attenuated, may still be able to express virulence-associated factors. Our aim was to screen for efficient and safe candidate strains that could be used as alternatives to E...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29490169/uropathogenic-escherichia-coli-virulence-factor-hemolysin-a-causes-programmed-cell-necrosis-by-altering-mitochondrial-dynamics
#15
Yongning Lu, Amir Rafiq, Zhengguo Zhang, Ferial Aslani, Monika Fijak, Tao Lei, Ming Wang, Sanjeev Kumar, Jörg Klug, Martin Bergmann, Trinad Chakraborty, Andreas Meinhardt, Sudhanshu Bhushan
Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections. In this study, UPEC strains harboring hemolysin A (HlyA) did not induce programmed cell death pathways by the activation of caspases. Instead, the UPEC pore-forming toxin HlyA triggered an increase in mitochondrial Ca2+ levels and manipulated mitochondrial dynamics by causing fragmentation of the mitochondrial network. Alterations in mitochondrial dynamics resulted in severe impairment of mitochondrial functions by loss of membrane potential, increase in ROS production, and ATP depletion...
February 28, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29479978/biofilm-formation-in-uropathogenic-escherichia-coli-association-with-adhesion-factor-genes
#16
Hojjatolah Zamani, Ali Salehzadeh
Background/aim: Biofilm formation is a major determinant factor in the development of urinary tract infections (UTIs) by uropathogenic Escherichia coli (UPEC). Several adhesion factors are involved in attachment of bacterial cells to the urinary tract and biofilm development. Materials and methods: The possible relationship between different adhesion factor genes (AFGs) and biofilm formation among UPEC isolates was investigated. Prevalence of different AFGs including fimA, fimH, papAH, papC, papEF, sfa-S, foc/G, and bmaE and their association to biofilm formation were investigated...
February 23, 2018: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29453908/effective-anti-adhesives-of-uropathogenic-escherichia-coli
#17
Rosana Ribić, Tomislav Meštrović, Marijana Neuberg, Goran Kozina
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are among the most common infectious diseases in humans. Due to their frequent occurrence in the community and nosocomial settings, as well as the development of resistance to the commonly prescribed antimicrobial agents, an enormous financial burden is placed on healthcare systems around the world. Therefore, novel approaches to the prevention and treatment of UTIs are needed. Although UPEC may harbour a plethora of virulence factors, type I fimbriae and P pili are two of the most studied adhesive organelles, since the attachment to host cells in the urinary tract is a crucial step towards infection...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29436416/persistent-pandemic-lineages-of-uropathogenic-escherichia-coli-in-a-college-community-from-1999-to-2017
#18
Reina Yamaji, Julia Rubin, Erika Thys, Cindy R Friedman, Lee W Riley
The incidence of drug-resistant community-acquired urinary tract infections (CA-UTI) continues to increase worldwide. In 1999 to 2000, a single lineage of uropathogenic Escherichia coli (UPEC) sequence type 69 (ST69) caused 51% of trimethoprim-sulfamethoxazole-resistant UTI in a Northern California university community. We compared the clonal distributions of UPEC and its impact on antimicrobial resistance prevalence in the same community during two periods separated by 17 years. We analyzed E. coli isolates from urine samples from patients with symptoms of UTI who visited a health service between September 2016 and May 2017 and compared them to UPEC isolates collected similarly between October 1999 and March 2000...
April 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29428427/horizontal-transfer-of-class-1-integrons-from-uropathogenic-escherichia-coli-to-e-coli-k12
#19
María Eloísa Poey, Magela Laviña
Class 1 integrons are genetic elements that carry a variable set of antibiotic resistance genes, being frequently found in clinical Gram-negative isolates. It is generally assumed that they easily spread horizontally among bacteria, thus contributing to the appearance of multidrug resistant clones. However, there are few experimental studies on the lateral transfer of these elements performed with bacterial collections that had been gathered following an epidemiological design. In this work, a collection with these characteristics, comprising uropathogenic Escherichia coli (UPEC) isolates bearing class 1 integrons, was employed to study the horizontal transfer of the integron to an E...
April 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29414075/cost-effective-flow-through-nanohole-array-based-biosensing-platform-for-the-label-free-detection-of-uropathogenic-e-coli-in-real-time
#20
Juan Gomez-Cruz, Srijit Nair, Angel Manjarrez-Hernandez, Sandra Gavilanes-Parra, Gabriel Ascanio, Carlos Escobedo
Rapid, inexpensive and sensitive detection of uropathogenic Escherichia coli (UPEC), a common cause of ascending urinary tract infections (UTIs) including cystitis and pyelonephritis, is critical given the increasing number of cases and its recurrence worldwide. In this paper, we present a label-free nanoplasmonic sensing platform, built with off-the-shelf optical and electronic components, which can detect intact UPEC at concentrations lower than the physiological limit for UTI diagnosis, in real time. The sensing platform consists of a red LED light source, lens assembly, CMOS detector, Raspberry Pi interface in conjugation with a metallic flow-through nanohole array-based sensor...
May 30, 2018: Biosensors & Bioelectronics
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