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https://www.readbyqxmd.com/read/28104383/virulence-traits-and-pathogenicity-of-uropathogenic-escherichia-coli-isolates-with-common-and-uncommon-o-serotypes
#1
Qingqing Gao, Debao Zhang, Zhengqin Ye, Xiaoping Zhu, Weixia Yang, Lanmei Dong, Song Gao, Xiufan Liu
Urinary tract infections (UTIs) are among the most common human diseases worldwide. This study aimed to collect uropathogenic Escherichia coli (UPEC) isolates from Jiangsu Province and obtain insights into the molecular epidemiology of UPEC in this region. The O serotypes, phylogenetic groups, and virulence factors of 183 UPEC isolates were determined. In this study, we isolated 51 UPEC isolates with common O serotypes including O1, O2, O4, O6, O7, O16, O18 and O75, as well as 35 of those with uncommonly encountered O serotypes including O8, O12, O15, O26, and O74...
January 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28087472/extract-of-clinopodium-bolivianum-protects-against-e-coli-invasion-of-uroepithelial-cells
#2
Soumitra Mohanty, Witchuda Kamolvit, Silvia Zambrana, Corine Sandström, Eduardo Gonzales, Claes-Göran Östenson, Annelie Braunerf
ETHNOPHARMACOLOGICAL RELEVANCE: Clinopodium bolivianum is a South American plant with anti-inflammatory and anti-infective activities. The increasing antibiotic resistance urges for alternative therapy. Based on its use in traditional medicine, we investigated the effect of C. bolivianum on the ability to defend bladder epithelial cells from E. coli infection. MATERIALS AND METHODS: The extract was analysed by LC-MS. Bladder epithelial cell lines T24 and 5637 and uropathogenic E...
January 10, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28074004/signaling-by-two-component-system-noncognate-partners-promotes-intrinsic-tolerance-to-polymyxin-b-in-uropathogenic-escherichia-coli
#3
Kirsten R Guckes, Erin J Breland, Ellisa W Zhang, Sarah C Hanks, Navleen K Gill, Holly M S Algood, Jonathan E Schmitz, Charles W Stratton, Maria Hadjifrangiskou
Bacteria use two-component systems (TCSs) to react appropriately to environmental stimuli. Typical TCSs comprise a sensor histidine kinase that acts as a receptor coupled to a partner response regulator that coordinates changes in bacterial behavior, often through its activity as a transcriptional regulator. TCS interactions are typically confined to cognate pairs of histidine kinases and response regulators. We describe two distinct TCSs in uropathogenic Escherichia coli (UPEC) that interact to mediate a response to ferric iron...
January 10, 2017: Science Signaling
https://www.readbyqxmd.com/read/28063174/oral-supplementation-of-trans-cinnamaldehyde-reduces-uropathogenic-escherichia-coli-colonization-in-a-mouse-model
#4
Amoolya Narayanan, Muhammed Shafeekh Muyyarikkandy, Shankumar Mooyottu, Kumar Venkitanarayanan, Mary Anne Roshni Amalaradjou
Urinary tract infections (UTIs) in the United States result in more than 7 million hospital visits per year. Uropathogenic E. coli (UPEC) is responsible for more than 80% of UTIs. Although antibiotics are the drug of choice to control UTIs, their repeated use has resulted in the emergence of antibiotic resistant UPEC. Thus, there is a need for effective alternate strategies to control UPEC infections. This study investigated the efficacy of trans-cinnamaldehye (TC), a food-grade molecule present in cinnamon, in reducing UPEC colonization and pathogenesis in the lower UTI...
January 7, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28040531/traditionally-used-medicinal-plants-against-uncomplicated-urinary-tract-infections-hexadecyl-coumaric-acid-ester-from-the-rhizomes-of-agropyron-repens-l-p-beauv-with-antiadhesive-activity-against-uropathogenic-e-coli
#5
Shabnam Sarshar Beydokthi, Jandirk Sendker, Simone Brandt, Andreas Hensel
The rhizomes from Agropyron repens are traditionally used for the treatment of uncomplicated urinary tract infections. Extracts prepared with solvents of different polarity did not show any cytotoxic effects against different strains of uropathogenic E. coli (UPEC) and human T24 bladder cells under in vitro conditions. Significant antiadhesive activity against the bacterial attachment to human T24 bladder cells was found for an acetone extract (AAE) at concentrations >250μg/mL. More hydrophilic extracts did not influence the bacterial attachment to the eukaryotic host cells...
December 29, 2016: Fitoterapia
https://www.readbyqxmd.com/read/28031261/copper-is-a-host-effector-mobilized-to-urine-during-urinary-tract-infection-to-impair-bacterial-colonization
#6
Amanda N Hyre, Kylie Kavanagh, Nancy D Kock, George L Donati, Sargurunathan Subashchandrabose
Urinary tract infection (UTI) is a major global infectious disease affecting millions of people annually. Human urinary copper (Cu) content is elevated during UTI caused by uropathogenic E. coli (UPEC). UPEC upregulate the expression of Cu efflux genes during clinical UTI in patients as an adaptive response to host-derived Cu. Whether Cu is mobilized to urine as a host response to UTI and its role in protection against UTI remain unresolved. To address these questions, we tested the hypothesis that Cu is a host effector mobilized to urine during UTI to limit bacterial growth...
December 28, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/28028717/multi-drug-resistant-uropathogenic-escherichia-coli-and-its-treatment-by-chinese-medicine
#7
Shi-Wei Liu, Xiao-Yang Xu, Jie Xu, Jiu-Yun Yuan, Wei-Kang Wu, Ning Zhang, Ze-Liang Chen
OBJECTIVES: To investigate the resistance and virulence profiles of uropathogenic Escherichia coli (UPEC) and its treatment by Chinese medicine (CM) Fuzheng Qingre Lishi Formula (, FQLF). METHODS: UPEC strains were isolated from recurrent urinary tract infections (UTIs) patients. Patient sensitivities to 17 antibiotics were tested by the disk diffusion method. Virulence genes were screened by plolymerase chain reaction. A mouse model was constructed using a multi-drug resistant and virulent UPEC strain and treated with FQLF or the antibiotic imipenem...
December 27, 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/27993701/comprehensive-expression-analysis-of-pathogenicity-genes-in-uropathogenic-escherichia-coli-strains
#8
Gloria Luz Paniagua-Contreras, Tania Hernández-Jaimes, Eric Monroy-Pérez, Felipe Vaca-Paniagua, Clara Díaz-Velásquez, Alina Uribe-García, Sergio Vaca
In this study, we investigated distinct expression patterns of genes encoding iron-acquisition systems, adhesins, protectins, and toxins in human uroepithelial cells infected with 194 uropathogenic Escherichia coli (UPEC) strains in vitro. We assessed the association of these genes with antibiotic resistance genes in this group of UPEC strains, previously characterised by polymerase chain reaction (PCR). Strains were isolated from patients with urinary tract infections (UTIs) from Unidad Médica Familiar de Salud Pública, located in Estado de México, México...
December 18, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27980776/macrophagic-control-of-the-response-to-uropathogenic-e-coli-infection-by-regulation-of-iron-retention-in-an-il-6-dependent-manner
#9
Nana Owusu-Boaitey, Kyle A Bauckman, Tingxuan Zhang, Indira U Mysorekar
INTRODUCTION: Uropathogenic Escherichia coli (UPEC), the causative agent of over 85% of urinary tract infections (UTIs), elaborate a number of siderophores to chelate iron from the host. On the other hand, the host immune imperative is to limit the availability of iron to the bacteria. Little is known regarding the mechanisms underlying this host-iron-UPEC interaction. Our objective was to determine whether macrophages, in response to UPEC infection, retain extracellular siderophore-bound and free iron, thus limiting the ability of UPEC to access iron...
December 2016: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/27934822/distribution-of-virulence-determinants-among-antimicrobial-resistant-and-antimicrobial-susceptible-escherichia-coli-implicated-in-urinary-tract-infections
#10
Sam Stephenson, P D Brown
INTRODUCTION: Uropathogenic Escherichia coli (UPEC) rely on the correlation of virulence expression with antimicrobial resistance to persist and cause severe urinary tract infections (UTIs). OBJECTIVES: We assessed the virulence pattern and prevalence among UPEC strains susceptible and resistant to multiple antimicrobial classes. METHODS: A total of 174 non-duplicate UPEC strains from patients with clinically significant UTIs were analysed for susceptibility to aminoglycoside, antifolate, cephalosporin, nitrofuran and quinolone antibiotics for the production of extended-spectrum β-lactamases and for the presence of six virulence determinants encoding adhesins (afimbrial, Type 1 fimbriae, P and S-fimbriae) and toxins (cytotoxic necrotising factor and haemolysin)...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27916544/characterization-of-escherichia-coli-causing-community-acquired-urinary-tract-infections-in-mexico-city
#11
Laura Belmont-Monroy, Rosa María Ribas-Aparicio, Armando Navarro-Ocaña, H Ángel Manjarrez-Hernández, Sandra Gavilanes-Parra, Gerardo Aparicio-Ozores, Patricia Isidra Cauich-Sánchez, Ulises Garza-Ramos, José Molina-López
The O25-ST131 clone was identified within 169 uropathogenic Escherichia coli (UPEC) strains. The 44.8% of the 29 O25-ST131 clones detected were positive to least to one extended-spectrum β-lactamase gene. The phylogroup D was mainly found. The O25-ST131 clone appeared to be associated with community-acquired UTI in Mexico City.
February 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27902433/escherichia-coli-clonal-group-a-among-uropathogenic-infections-in-mexico-city
#12
Angel Manjarrez-Hernandez, José Molina-López, Sandra Gavilanes-Parra, Rigoberto Hernandez-Castro
Escherichia coli clonal group A (CGA) causes urinary tract and other extra-intestinal infections in humans. CGA is an important cause of trimethoprim/sulfamethoxazole (SXT) resistance in extra-intestinal pathogens. We examined the extent to which resistance in this area is related to CGA dissemination of E. coli from urinary tract infections (UTIs) in Mexico City. The virulence backgrounds of the isolates were also characterized. In this study, the frequency of resistance to SXT used for UTI treatment was high (56-65 %), and CGA isolates accounted for 9 of the 78 SXT-resistant isolates (11...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27843814/dimeric-and-trimeric-fusion-proteins-generated-with-fimbrial-adhesins-of-uropathogenic-escherichia-coli
#13
Víctor M Luna-Pineda, Juan Pablo Reyes-Grajeda, Ariadnna Cruz-Córdova, Zeus Saldaña-Ahuactzi, Sara A Ochoa, Carmen Maldonado-Bernal, Vicenta Cázares-Domínguez, Leticia Moreno-Fierros, José Arellano-Galindo, Rigoberto Hernández-Castro, Juan Xicohtencatl-Cortes
Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, and uropathogenic Escherichia coli (UPEC) is the main etiologic agent. Fimbriae assembled on the bacterial surface are essential for adhesion to the urinary tract epithelium. In this study, the FimH, CsgA, and PapG adhesins were fused to generate biomolecules for use as potential target vaccines against UTIs. The fusion protein design was generated using bioinformatics tools, and template fusion gene sequences were synthesized by GenScript in the following order fimH-csgA-papG-fimH-csgA (fcpfc) linked to the nucleotide sequence encoding the [EAAAK]5 peptide...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27825516/adaptation-of-escherichia-coli-traversing-from-the-faecal-environment-to-the-urinary-tract
#14
Karen L Nielsen, Marc Stegger, Paul A Godfrey, Michael Feldgarden, Paal S Andersen, Niels Frimodt-Møller
The majority of extraintestinal pathogenic Escherichia coli (ExPEC) causing urinary tract infections (UTI) are found in the patient's own gut flora, but only limited knowledge is available on the potential adaptation that may occur in the bacteria in order to traverse the perineum and successfully infect the urinary tract. Here, matching pairs of faecal and UTI isolates from 42 patients were compared pairwise using in-depth whole-genome sequencing to investigate whether genetic changes were evident for successful colonization in these two different environments...
December 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27821778/siderophore-vaccine-conjugates-protect-against-uropathogenic-escherichia-coli-urinary-tract-infection
#15
Laura A Mike, Sara N Smith, Christopher A Sumner, Kathryn A Eaton, Harry L T Mobley
Uropathogenic Escherichia coli (UPEC) is the primary cause of uncomplicated urinary tract infections (UTIs). Whereas most infections are isolated cases, 1 in 40 women experience recurrent UTIs. The rise in antibiotic resistance has complicated the management of chronic UTIs and necessitates new preventative strategies. Currently, no UTI vaccines are approved for use in the United States, and the development of a highly effective vaccine remains elusive. Here, we have pursued a strategy for eliciting protective immunity by vaccinating with small molecules required for pathogenesis, rather than proteins or peptides...
November 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27801366/evaluation-of-paiusp-subtyping-to-characterize-uropathogenic-e-coli-isolates
#16
Yun Mei Lai, Myo Thura Zaw, Shamsul Bahari Shamsudin, Zaw Lin
INTRODUCTION: Uropathogenic virulence factors have been identified by comparing the prevalence of these among urinary tract isolates and environmental strains. The uropathogenic-specific protein (USP) gene is present on the pathogenicity island (PAI) of uropathogenic Escherichia coli (UPEC) and, depending on its two diverse gene types and the sequential patterns of three open reading frame units (orfUs) following it, there is a method to characterize UPEC epidemiologically called PAIusp subtyping...
October 31, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27795353/purine-biosynthesis-metabolically-constrains-intracellular-survival-of-uropathogenic-escherichia-coli
#17
Carrie L Shaffer, Ellisa W Zhang, Anne G Dudley, Beverly R E A Dixon, Kirsten R Guckes, Erin J Breland, Kyle A Floyd, Daniel P Casella, Holly M Scott Algood, Douglass B Clayton, Maria Hadjifrangiskou
The ability to de novo synthesize purines has been associated with the intracellular survival of multiple bacterial pathogens. Uropathogenic Escherichia coli (UPEC), the predominant cause of urinary tract infections, undergoes a transient intracellular lifestyle during which bacteria clonally expand into multicellular bacterial communities within the cytoplasm of bladder epithelial cells. Here, we characterized the contribution of the conserved de novo purine biosynthesis-associated locus cvpA-purF to UPEC pathogenesis...
January 2017: Infection and Immunity
https://www.readbyqxmd.com/read/27767067/the-cytochrome-bd-i-respiratory-oxidase-augments-survival-of-multidrug-resistant-escherichia-coli-during-infection
#18
Mark Shepherd, Maud E S Achard, Adi Idris, Makrina Totsika, Minh-Duy Phan, Kate M Peters, Sohinee Sarkar, Cláudia A Ribeiro, Louise V Holyoake, Dimitrios Ladakis, Glen C Ulett, Matthew J Sweet, Robert K Poole, Alastair G McEwan, Mark A Schembri
Nitric oxide (NO) is a toxic free radical produced by neutrophils and macrophages in response to infection. Uropathogenic Escherichia coli (UPEC) induces a variety of defence mechanisms in response to NO, including direct NO detoxification (Hmp, NorVW, NrfA), iron-sulphur cluster repair (YtfE), and the expression of the NO-tolerant cytochrome bd-I respiratory oxidase (CydAB). The current study quantifies the relative contribution of these systems to UPEC growth and survival during infection. Loss of the flavohemoglobin Hmp and cytochrome bd-I elicit the greatest sensitivity to NO-mediated growth inhibition, whereas all but the periplasmic nitrite reductase NrfA provide protection against neutrophil killing and promote survival within activated macrophages...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27752899/persistence-of-uropathogenic-escherichia-coli-in-the-bladders-of-female-patients-with-sterile-urine-after-antibiotic-therapies
#19
Shu-Cheng Liu, Xiao-Min Han, Ming Shi, Zi-Li Pang
This study aimed to provide evidence of persistent uropathogenic Escherichia coli (UPEC) in female patients with recurrent urinary tract infection (UTI) after antibiotic therapy. We collected biopsies of the bladder, and clean-catch urine samples from 32 women who had episodes of recurrent UTI and were given antibiotic therapy. Urine samples and biopsies were analyzed by conventional bacteriological techniques. Phylogenetic group and 16 virulence factors (VFs) of UPEC were determined using polymerase chain reaction (PCR)...
October 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27744103/detection-of-ampc-%C3%AE-lactamase-and-adherence-factors-in-uropathogenic-escherichia-coli-isolated-from-aged-patients
#20
Santosh Kumar Singh, Kumari Seema, Minakshi Gupta
Escherichia coli mediated urinary tract infection has been reported to be most prevalent among patients of different class, gender and ages. Currently, multidrug resistant E. coli harboring several virulence factors are most perilous threats for patients especially for elders. The aim of this study was to determine the antibiotic resistance pattern, co-resistance and phenotypic virulence factors present in uropathogenic E. coli isolated from aged patients. Thirty-nine E. coli isolates were collected during May-June 2014 from patients between 50 to 80 years of age...
November 2016: Microbial Pathogenesis
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