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https://www.readbyqxmd.com/read/28631739/infection-impeding-upec-gut-colonization
#1
Clemens Thoma
No abstract text is available yet for this article.
June 20, 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/28614296/selective-depletion-of-uropathogenic-e-coli-from-the-gut-by-a-fimh-antagonist
#2
Caitlin N Spaulding, Roger D Klein, Ségolène Ruer, Andrew L Kau, Henry L Schreiber, Zachary T Cusumano, Karen W Dodson, Jerome S Pinkner, Daved H Fremont, James W Janetka, Han Remaut, Jeffrey I Gordon, Scott J Hultgren
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) affect 150 million people annually. Despite effective antibiotic therapy, 30-50% of patients experience recurrent UTIs. In addition, the growing prevalence of UPEC that are resistant to last-line antibiotic treatments, and more recently to carbapenems and colistin, make UTI a prime example of the antibiotic-resistance crisis and emphasize the need for new approaches to treat and prevent bacterial infections. UPEC strains establish reservoirs in the gut from which they are shed in the faeces, and can colonize the periurethral area or vagina and subsequently ascend through the urethra to the urinary tract, where they cause UTIs...
June 14, 2017: Nature
https://www.readbyqxmd.com/read/28594893/pandemic-extra-intestinal-pathogenic-escherichia-coli-expec-clonal-group-o6-b2-st73-as-a-cause-of-avian-colibacillosis-in-brazil
#3
Marcos Paulo Vieira Cunha, Andre Becker Saidenberg, Andrea Micke Moreno, Antonio José Piantino Ferreira, Mônica Aparecida Midolli Vieira, Tânia Aparecida Tardelli Gomes, Terezinha Knöbl
Extra-intestinal pathogenic Escherichia coli (ExPEC) represent an emerging pathogen, with pandemic strains increasingly involved in cases of urinary tract infections (UTIs), bacteremia, and meningitis. In addition to affecting humans, the avian pathotype of ExPEC, avian pathogenic E. coli (APEC), causes severe economic losses to the poultry industry. Several studies have revealed overlapping characteristics between APEC and human ExPEC, leading to the hypothesis of a zoonotic potential of poultry strains. However, the description of certain important pandemic clones, such as Sequence Type 73 (ST73), has not been reported in food sources...
2017: PloS One
https://www.readbyqxmd.com/read/28591134/global-gene-expression-profiling-and-antibiotic-susceptibility-after-repeated-exposure-to-the-carbon-monoxide-releasing-molecule-2-corm-2-in-multidrug-resistant-esbl-producing-uropathogenic-escherichia-coli
#4
Charlotte Sahlberg Bang, Isak Demirel, Robert Kruse, Katarina Persson
Treatment of urinary tract infections is today a challenge due to the increasing prevalence of multidrug-resistant ESBL-producing uropathogenic Escherichia coli (UPEC). There is an urgent need for new treatment strategies for multidrug-resistant UPEC and preferably with targets that have low potential for development of resistance. Carbon monoxide-releasing molecules (CORMs) are novel and potent antibacterial agents. The present study examines the transcriptomic targets of CORM-2 in a multidrug-resistant ESBL-producing UPEC isolate in response to a single exposure to CORM-2 and after repeated exposure to CORM-2...
2017: PloS One
https://www.readbyqxmd.com/read/28550944/-omic-approaches-to-study-uropathogenic-escherichia-coli-virulence
#5
REVIEW
Alvin W Lo, Danilo G Moriel, Minh-Duy Phan, Benjamin L Schulz, Timothy J Kidd, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is a pathogen of major significance to global human health and is strongly associated with rapidly increasing antibiotic resistance. UPEC is the primary cause of urinary tract infection (UTI), a disease that involves a complicated pathogenic pathway of extracellular and intracellular lifestyles during interaction with the host. The application of multiple 'omic' technologies, including genomics, transcriptomics, proteomics, and metabolomics, has provided enormous knowledge to our understanding of UPEC biology...
May 24, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28545489/cytotoxic-necrotizing-factor-1-cnf1-does-not-promote-e-coli-infection-in-a-murine-model-of-ascending-pyelonephritis
#6
Jason E Michaud, Kwang Sik Kim, William Harty, Matthew Kasprenski, Ming-Hsien Wang
BACKGROUND: Urinary tract infections (UTI) are among the most common and costly infections in both hospitalized and ambulatory patients. Uropathogenic E. coli (UPEC) represent the majority of UTI isolates and are a diverse group of bacteria that utilize a variety of virulence factors to establish infection of the genitourinary tract. The virulence factor cytotoxic necrotizing factor-1 (CNF1) is frequently expressed in clinical UPEC isolates. To date, there have been conflicting reports on the role of CNF1 in the pathogenesis of E...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28536675/an-overview-of-two-component-signal-transduction-systems-implicated-in-extra-intestinal-pathogenic-e-coli-infections
#7
REVIEW
Erin J Breland, Allison R Eberly, Maria Hadjifrangiskou
Extra-intestinal pathogenic E. coli (ExPEC) infections are common in mammals and birds. The predominant ExPEC types are avian pathogenic E. coli (APEC), neonatal meningitis causing E. coli/meningitis associated E. coli (NMEC/MAEC), and uropathogenic E. coli (UPEC). Many reviews have described current knowledge on ExPEC infection strategies and virulence factors, especially for UPEC. However, surprisingly little has been reported on the regulatory modules that have been identified as critical in ExPEC pathogenesis...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28536290/peptidoglycan-association-of-murein-lipoprotein-is-required-for-kpsd-dependent-group-2-capsular-polysaccharide-expression-and-serum-resistance-in-a-uropathogenic-escherichia-coli-isolate
#8
Jingyu Diao, Catrien Bouwman, Donghong Yan, Jing Kang, Anand K Katakam, Peter Liu, Homer Pantua, Alexander R Abbas, Nicholas N Nickerson, Cary Austin, Mike Reichelt, Wendy Sandoval, Min Xu, Chris Whitfield, Sharookh B Kapadia
Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mechanism has not been established. Here, lpp and pal mutants of uropathogenic E. coli strain CFT073 showed reduced survival in a mouse bacteremia model, but only the lpp mutant was sensitive to serum killing in vitro The peptidoglycan-bound Lpp form was specifically required for preventing complement-mediated bacterial lysis in vitro and complement-mediated clearance in vivo Compared to the wild-type strain, the lpp mutant had impaired K2 capsular polysaccharide production and was unable to respond to exposure to serum by elevating capsular polysaccharide amounts...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#9
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
June 2, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28491245/frequency-evaluation-of-genes-encoding-siderophores-and-the-effects-of-different-concentrations-of-fe-ions-on-growth-rate-of-uropathogenic-escherichia-coli
#10
Behnoush Khasheii, Shaghayegh Anvari, Ailar Jamalli
BACKGROUND AND OBJECTIVES: Bacteria need iron for growth and most of them can actively acquire Fe ions using especial iron-chelating proteins which named siderophores. We aimed to determine the frequencies of iucA, iroN and irp2 genes in the uropathogenic Escherichia coli (UPEC) isolates. We also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. MATERIALS AND METHODS: Totally, 170 E. coli strains were isolated from urinary tract infections and the presence of 3 siderophore genes were analyzed using PCR among them...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28489862/novel-genes-associated-with-enhanced-motility-of-escherichia-coli-st131
#11
Asha Kakkanat, Minh-Duy Phan, Alvin W Lo, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is the cause of ~75% of all urinary tract infections (UTIs) and is increasingly associated with multidrug resistance. This includes UPEC strains from the recently emerged and globally disseminated sequence type 131 (ST131), which is now the dominant fluoroquinolone-resistant UPEC clone worldwide. Most ST131 strains are motile and produce H4-type flagella. Here, we applied a combination of saturated Tn5 mutagenesis and transposon directed insertion site sequencing (TraDIS) as a high throughput genetic screen and identified 30 genes associated with enhanced motility of the reference ST131 strain EC958...
2017: PloS One
https://www.readbyqxmd.com/read/28485709/occurrence-and-characterization-of-class-1-integrons-in-escherichia-coli-from-healthy-individuals-and-those-with-urinary-infection
#12
Clarisse Oliveira-Pinto, Cristiane Diamantino, Patrícia L Oliveira, Mariana P Reis, Patrícia S Costa, Magna C Paiva, Regina M D Nardi, Paula P Magalhães, Edmar Chartone-Souza, Andréa M A Nascimento
PURPOSE: Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40). METHODOLOGY: The methods used included PCR, sequencing and antimicrobial susceptibility testing...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28478807/aqueous-extract-from-orthosiphon-stamineus-leaves-prevents-bladder-and-kidney-infection-in-mice
#13
S Sarshar, S Brandt, M R Asadi Karam, M Habibi, S Bouzari, M Lechtenberg, U Dobrindt, X Qin, F M Goycoolea, A Hensel
BACKGROUND: Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. PURPOSES: Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. STUDY DESIGN: In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing...
May 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28468965/cell-specific-qrt-pcr-of-renal-epithelial-cells-reveals-a-novel-innate-immune-signature-in-murine-collecting-duct
#14
Vijay Saxena, David S Hains, John Ketz, Melinda Chanley, John D Spencer, Brian Becknell, Keith R Pierce, Raoul D Nelson, Jeffrey M Purkerson, George J Schwartz, Andrew L Schwaderer
The urinary tract is usually culture negative despite its close proximity to microbial flora. The precise mechanisms by which the kidneys and urinary tract defends against infection is not well understood. The initial kidney cells to encounter ascending pathogens are the collecting tubule cells which consist of principal cells (PCs) that express aquaporin 2 (AQP2) and intercalated cells (ICs) that express vacuolar H+-ATPase (V-ATPase, B1 subunit). We have previously shown that ICs are involved with the human renal innate immune defense...
May 3, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28438119/dfi-seq-identification-of-environment-specific-gene-expression-in-uropathogenic-escherichia-coli
#15
Michelle Madelung, Tina Kronborg, Thomas Koed Doktor, Carsten Struve, Karen Angeliki Krogfelt, Jakob Møller-Jensen
BACKGROUND: During infection of the urinary tract, uropathogenic Escherichia coli (UPEC) are exposed to different environments, such as human urine and the intracellular environments of bladder epithelial cells. Each environment elicits a distinct bacterial environment-specific transcriptional response. We combined differential fluorescence induction (DFI) with next-generation sequencing, collectively termed DFI-seq, to identify differentially expressed genes in UPEC strain UTI89 during growth in human urine and bladder cells...
April 24, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28419868/ciprofloxacin-resistance-in-uropathogenic-escherichia-coli-isolates-causing-community-acquired-urinary-infections-in-bras%C3%A3-lia-brazil
#16
Rafaella Christina Rocha Moreira da Silva, Paulo de Oliveira Martins Júnior, Laura Fernandes Gonçalves, Vicente de Paulo Martins, Ana Beatriz Fabrício de Melo, André Pitondo-Silva, Tatiana Amabile de Campos
OBJECTIVES: Considering the global concern of ciprofloxacin resistance, the aim of this study was to evaluate the characteristics of ciprofloxacin-resistant (CIP-R) Escherichia coli isolated from patients with community-acquired urinary tract infections (UTIs) in Brasília, Brazil. METHODS: CIP-R E. coli isolated from different outpatients between July 2013 and April 2014 in a tertiary hospital were analysed for antibiotic resistance profile, phylotype, uropathogenic E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28396353/the-histidine-residue-of-qsec-is-required-for-canonical-signaling-between-qseb-and-pmrb-in-uropathogenic-escherichia-coli
#17
Erin J Breland, Ellisa W Zhang, Tomas Bermudez, Charles R Martinez, Maria Hadjifrangiskou
Two-component systems are prototypically comprised of a histidine kinase (sensor) and a response regulator (responder). The sensor kinases auto-phosphorylate at a conserved histidine residue, acting as a phospho-donor for subsequent phosphotransfer to and activation of a cognate response regulator. In rare cases, the histidine residue is also essential for response regulator de-phosphorylation via a reverse-phosphotransfer reaction. In this work, we present an example of a kinase that relies on reverse-phosphotransfer to catalyze the de-phosphorylation of its cognate partner...
April 10, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28359738/development-of-a-multiplex-real-time-pcr-assay-for-phylogenetic-analysis-of-uropathogenic-escherichia-coli
#18
Mojtaba Hasanpour, Akram Najafi
Uropathogenic Escherichia coli (UPEC) is among major pathogens causing 80-90% of all episodes of urinary tract infections (UTIs). Recently, E. coli strains are divided into eight main phylogenetic groups including A, B1, B2, C, D, E, F, and clade I. This study was aimed to develop a rapid, sensitive, and specific multiplex real time PCR method capable of detecting phylogenetic groups of E. coli strains. This study was carried out on E. coli strains (isolated from the patient with UTI) in which the presence of all seven target genes had been confirmed in our previous phylogenetic study...
March 28, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28330863/bacterial-virulence-phenotypes-of-escherichia-coli-and-host-susceptibility-determine-risk-for-urinary-tract-infections
#19
Henry L Schreiber, Matt S Conover, Wen-Chi Chou, Michael E Hibbing, Abigail L Manson, Karen W Dodson, Thomas J Hannan, Pacita L Roberts, Ann E Stapleton, Thomas M Hooton, Jonathan Livny, Ashlee M Earl, Scott J Hultgren
Urinary tract infections (UTIs) are caused by uropathogenic Escherichia coli (UPEC) strains. In contrast to many enteric E. coli pathogroups, no genetic signature has been identified for UPEC strains. We conducted a high-resolution comparative genomic study using E. coli isolates collected from the urine of women suffering from frequent recurrent UTIs. These isolates were genetically diverse and varied in their urovirulence, that is, their ability to infect the bladder in a mouse model of cystitis. We found no set of genes, including previously defined putative urovirulence factors (PUFs), that were predictive of urovirulence...
March 22, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28302076/uropathogenic-escherichia-coli-pathogenicity-islands-and-other-expec-virulence-genes-may-contribute-to-the-genome-variability-of-enteroinvasive-e-coli
#20
Laís Cristina da Silva, Ana Carolina de Mello Santos, Rosa Maria Silva
BACKGROUND: Enteroinvasive Escherichia coli (EIEC) may be the causative agent of part of those million cases of diarrhea illness reported worldwide every year and attributable to Shigella. That is because both enteropathogens have many common characteristics that difficult their identification either by traditional microbiological methods or by molecular tools used in the clinical laboratory settings. While Shigella has been extensively studied, EIEC remains barely characterized at the molecular level...
March 16, 2017: BMC Microbiology
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