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https://www.readbyqxmd.com/read/27904884/escherichia-coli-sequence-type-131-h30-is-the-main-driver-of-emerging-extended-spectrum-%C3%AE-lactamase-producing-e-coli-at-a-tertiary-care-center
#1
James R Johnson, Brian Johnston, Paul Thuras, Bryn Launer, Evgeni V Sokurenko, Loren G Miller
The H30 strain of Escherichia coli sequence type 131 (ST131-H30) is a recently emerged, globally disseminated lineage associated with fluoroquinolone resistance and, via its H30Rx subclone, the CTX-M-15 extended-spectrum beta-lactamase (ESBL). Here, we studied the clonal background and resistance characteristics of 109 consecutive recent E. coli clinical isolates (2015) and 41 historical ESBL-producing E. coli blood isolates (2004 to 2011) from a public tertiary care center in California with a rising prevalence of ESBL-producing E...
November 2016: MSphere
https://www.readbyqxmd.com/read/27865830/community-onset-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-sequence-type-131-at-two-korean-community-hospitals-the-spread-of-multidrug-resistant-e-coli-to-the-community-via-healthcare-facilities
#2
Young Ah Kim, Jin Ju Kim, Heejung Kim, Kyungwon Lee
BACKGROUND: The recent molecular epidemiology of ESBL-producing Escherichia coli infection in two Korean community hospitals was evaluated in this prospective observational study. METHODS: We collected non-duplicated E. coli isolates from consecutive, sequentially encountered patients with community-onset episodes between March and April 2016 in two community hospitals in Gyeonggi-do province, Korea. We studied the prevalence, clinical characteristics and molecular epidemiology of E...
November 16, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27830191/comparative-genome-analysis-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-sequence-type-131-strains-from-nepal-and-japan
#3
Tohru Miyoshi-Akiyama, Jatan Bahadur Sherchan, Yohei Doi, Maki Nagamatsu, Jeevan B Sherchand, Sarmila Tandukar, Norio Ohmagari, Teruo Kirikae, Hiroshi Ohara, Kayoko Hayakawa
The global spread of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli (ESBL-E. coli) has largely been driven by the pandemic sequence type 131 (ST131). This study aimed to determine the molecular epidemiology of their spread in two Asian countries with contrasting prevalence. We conducted whole-genome sequencing (WGS) of ESBL-E. coli ST131 strains collected prospectively from Nepal and Japan, two countries in Asia with a high and low prevalence of ESBL-E. coli, respectively. We also systematically compared these genomes with those reported from other regions using publicly available WGS data for E...
September 2016: MSphere
https://www.readbyqxmd.com/read/27767006/global-escherichia-coli-sequence-type-131-clade-with-blactx-m-27-gene
#4
Yasufumi Matsumura, Johann D D Pitout, Ryota Gomi, Tomonari Matsuda, Taro Noguchi, Masaki Yamamoto, Gisele Peirano, Rebekah DeVinney, Patricia A Bradford, Mary R Motyl, Michio Tanaka, Miki Nagao, Shunji Takakura, Satoshi Ichiyama
The Escherichia coli sequence type (ST) 131 C2/H30Rx clade with the blaCTX-M-15 gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. ST131 C1/H30R with blaCTX-M-27 emerged among ESBL-producing E. coli in Japan during the late 2000s. To investigate the possible expansion of a single clade, we performed whole-genome sequencing for 43 Japan and 10 global ST131 isolates with blaCTX-M-27 (n = 16), blaCTX-M-14 (n = 16), blaCTX-M-15 (n = 13), and others (n = 8)...
November 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27703999/the-niche-for-escherichia-coli-sequence-type-131-among-veterans-urinary-tract-abnormalities-and-long-term-care-facilities
#5
Dimitri M Drekonja, Michael A Kuskowski, Ruth Anway, Brian D Johnston, James R Johnson
Background.  Antimicrobial resistance among Escherichia coli is increasing, driven largely by the global emergence of sequence type 131 (ST131). However, the clinical significance of ST131 status is unknown. Among veterans, we assessed whether ST131 causes more severe, persistent, or recurrence-prone infections than non-ST131 E. coli. Methods.  Isolates were assessed by polymerase chain reaction for membership in ST131 and relevant subclones thereof (H30R and H30Rx) and by broth microdilution for susceptibility to 11 antibiotics...
September 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/27703993/household-clustering-of-escherichia-coli-sequence-type-131-clinical-and-fecal-isolates-according-to-whole-genome-sequence-analysis
#6
James R Johnson, Gregg Davis, Connie Clabots, Brian D Johnston, Stephen Porter, Chitrita DebRoy, William Pomputius, Peter T Ender, Michael Cooperstock, Billie Savvas Slater, Ritu Banerjee, Sybille Miller, Dagmara Kisiela, Evgeni V Sokurenko, Maliha Aziz, Lance B Price
Background.  Within-household sharing of strains from the resistance-associated H30R1 and H30Rx subclones of Escherichia coli sequence type 131 (ST131) has been inferred based on conventional typing data, but it has been assessed minimally using whole genome sequence (WGS) analysis. Methods.  Thirty-three clinical and fecal isolates of ST131-H30R1 and ST131-H30Rx, from 20 humans and pets in 6 households, underwent WGS analysis for comparison with 52 published ST131 genomes. Phylogenetic relationships were inferred using a bootstrapped maximum likelihood tree based on core genome sequence polymorphisms...
September 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/27494832/ctx-m-15-h30rx-st131-subclone-is-one-of-the-main-causes-of-healthcare-associated-esbl-producing-escherichia-coli-bacteraemia-of-urinary-origin-in-spain
#7
Irene Merino, Evelyn Shaw, Juan Pablo Horcajada, Emilia Cercenado, Beatriz Mirelis, M Angeles Pallarés, Juliá Gómez, M Xercavins, Luis Martínez-Martínez, Marina De Cueto, Rafael Cantón, Patricia Ruiz-Garbajosa
OBJECTIVES: The objective of this study was to assess the prevalence and molecular epidemiology of ESBL-producing Escherichia coli causing healthcare-associated (HCA) and community-associated (CA) bacteraemia of urinary origin (BUO) in Spain. METHODS: An observational cohort study was conducted at eight hospitals from different Spanish geographical areas (2010-11). BUO episodes (n = 425) were classified as HCA (n = 215) and CA (n = 210), and one blood isolate per episode was collected...
August 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27390780/separate-f-type-plasmids-have-shaped-the-evolution-of-the-h30-subclone-of-escherichia-coli-sequence-type-131
#8
Timothy J Johnson, Jessica L Danzeisen, Bonnie Youmans, Kyle Case, Katharine Llop, Jeannette Munoz-Aguayo, Cristian Flores-Figueroa, Maliha Aziz, Nicole Stoesser, Evgeni Sokurenko, Lance B Price, James R Johnson
The extraintestinal pathogenic Escherichia coli (ExPEC) H30 subclone of sequence type 131 (ST131-H30) has emerged abruptly as a dominant lineage of ExPEC responsible for human disease. The ST131-H30 lineage has been well described phylogenetically, yet its plasmid complement is not fully understood. Here, single-molecule, real-time sequencing was used to generate the complete plasmid sequences of ST131-H30 isolates and those belonging to other ST131 clades. Comparative analyses revealed separate F-type plasmids that have shaped the evolution of the main fluoroquinolone-resistant ST131-H30 clades...
July 2016: MSphere
https://www.readbyqxmd.com/read/27174264/complete-genome-sequence-of-a-ctx-m-15-producing-escherichia-coli-strain-from-the-h30rx-subclone-of-sequence-type-131-from-a-patient-with-recurrent-urinary-tract-infections-closely-related-to-a-lethal-urosepsis-isolate-from-the-patient-s-sister
#9
Timothy J Johnson, Maliha Aziz, Cindy M Liu, Evgeni Sokurenko, Dagmara I Kisiela, Sandip Paul, Paal Andersen, James R Johnson, Lance B Price
We report here the complete genome sequence, including five plasmid sequences, of Escherichia coli sequence type 131 (ST131) strain JJ1887. The strain was isolated in 2007 in the United States from a patient with recurrent cystitis, whose caregiver sister died from urosepsis caused by a nearly identical strain.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27006459/evolutionary-history-of-the-global-emergence-of-the-escherichia-coli-epidemic-clone-st131
#10
Nicole Stoesser, Anna E Sheppard, Louise Pankhurst, Nicola De Maio, Catrin E Moore, Robert Sebra, Paul Turner, Luke W Anson, Andrew Kasarskis, Elizabeth M Batty, Veronica Kos, Daniel J Wilson, Rattanaphone Phetsouvanh, David Wyllie, Evgeni Sokurenko, Amee R Manges, Timothy J Johnson, Lance B Price, Timothy E A Peto, James R Johnson, Xavier Didelot, A Sarah Walker, Derrick W Crook
UNLABELLED: Escherichia colisequence type 131 (ST131) has emerged globally as the most predominant extraintestinal pathogenic lineage within this clinically important species, and its association with fluoroquinolone and extended-spectrum cephalosporin resistance impacts significantly on treatment. The evolutionary histories of this lineage, and of important antimicrobial resistance elements within it, remain unclearly defined. This study of the largest worldwide collection (n= 215) of sequenced ST131E...
March 22, 2016: MBio
https://www.readbyqxmd.com/read/26643062/prevalence-and-characteristics-of-escherichia-coli-sequence-type-131-and-its-h30-and-h30rx-subclones-a-multicenter-study-from-korea
#11
MULTICENTER STUDY
Soo-Young Kim, Yeon-Joon Park, James R Johnson, Jin Kyung Yu, Yong-Kyun Kim, Yeong Sic Kim
We investigated the prevalence and characteristics of Escherichia coli sequence type 131 (ST131) and its subclones among 268 E. coli isolates. The isolates were collected from 21 Korean hospitals without use of selection criteria and were screened for ST131 status by PCR. ST131 isolates were characterized for extended-spectrum β-lactamase variants, fluoroquinolone resistance genes, plasmid addiction systems, and replicon types. The collection's 57 identified ST131 isolates (21% of 268) were distributed disproportionately by clonal subset, as follows: 21 (37%) H30Rx, 27 (47%) H30 non-Rx, and 8 (14%) non-H30...
February 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/26276899/erratum-for-johnson-et-al-intensity-and-mechanisms-of-fluoroquinolone-resistance-within-the-h30-and-h30rx-subclones-of-escherichia-coli-sequence-type-131-compared-with-other-fluoroquinolone-resistant-e-coli
#12
James R Johnson, Brian Johnston, Michael A Kuskowski, Evgeni V Sokurenko, Veronika Tchesnokova
No abstract text is available yet for this article.
September 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26100703/variation-in-resistance-traits-phylogenetic-backgrounds-and-virulence-genotypes-among-escherichia-coli-clinical-isolates-from-adjacent-hospital-campuses-serving-distinct-patient-populations
#13
Sarah M Drawz, Stephen Porter, Michael A Kuskowski, Brian Johnston, Connie Clabots, Susan Kline, Patricia Ferrieri, James R Johnson
Escherichia coli sequence type 13 (ST131), an emergent cause of multidrug-resistant extraintestinal infections, has important phylogenetic subsets, notably the H30 and H30Rx subclones, with distinctive resistance profiles and, possibly, clinical associations. To clarify the local prevalence of these ST131 subclones and their associations with antimicrobial resistance, ecological source, and virulence traits, we extensively characterized 233 consecutive E. coli clinical isolates (July and August 2013) from the University of Minnesota Medical Center-Fairview Infectious Diseases and Diagnostic Laboratory, Minneapolis, MN, which serves three adjacent facilities (a children's hospital and low- and high-acuity adult facilities)...
September 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25987621/intensity-and-mechanisms-of-fluoroquinolone-resistance-within-the-h30-and-h30rx-subclones-of-escherichia-coli-sequence-type-131-compared-with-other-fluoroquinolone-resistant-e-coli
#14
James R Johnson, Brian Johnston, Michael A Kuskowski, Evgeni V Sokurenko, Veronika Tchesnokova
The recent expansion of the H30 subclone of Escherichia coli sequence type 131 (ST131) and its CTX-M-15-associated H30Rx subset remains unexplained. Although ST131 H30 typically exhibits fluoroquinolone resistance, so do multiple other E. coli lineages that have not expanded similarly. To determine whether H30 isolates have more intense fluoroquinolone resistance than other fluoroquinolone-resistant E. coli isolates and to identify possible mechanisms, we determined the MICs for four fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin, and norfloxacin) among 89 well-characterized, genetically diverse fluoroquinolone-resistant E...
August 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25828998/extensive-household-outbreak-of-urinary-tract-infection-and-intestinal-colonization-due-to-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-sequence-type-131
#15
Theresa Madigan, James R Johnson, Connie Clabots, Brian D Johnston, Stephen B Porter, Billie S Slater, Ritu Banerjee
BACKGROUND: Reasons for the successful global dissemination of multidrug-resistant Escherichia coli sequence type 131 (ST131) are undefined, but may include enhanced transmissibility or ability to colonize the intestine compared with other strains. METHODS: We identified a household in which 2 young children had urinary tract infection (UTI) caused by an extended-spectrum β-lactamase (ESBL)-producing, multidrug-resistant ST131 E. coli strain. We assessed the prevalence of ST131 intestinal colonization among the 7 household members (6 humans, 1 dog)...
July 1, 2015: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/25687644/ctx-m-27-and-ctx-m-14-producing-ciprofloxacin-resistant-escherichia-coli-of-the-h30-subclonal-group-within-st131-drive-a-japanese-regional-esbl-epidemic
#16
Yasufumi Matsumura, James R Johnson, Masaki Yamamoto, Miki Nagao, Michio Tanaka, Shunji Takakura, Satoshi Ichiyama
OBJECTIVES: The global increase in ESBL-producing Escherichia coli is associated with the ST131 clonal group, especially its CTX-M-15-producing H30Rx subset. To understand the rapid spread of ESBL-producing E. coli in Japan, we investigated the molecular epidemiology and ESBL-associated genetic environments of Japanese ST131 isolates. METHODS: Between 2001 and 2012, 1079 ESBL-producing E. coli isolates were collected at 10 Japanese acute-care hospitals. ESBL types, ST131 status, fimH allele, H30Rx-defining sequences and ESBL-associated genetic environments were defined using PCR and sequencing...
2015: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/25269819/comparative-genome-analysis-identifies-few-traits-unique-to-the-escherichia-coli-st131-h30rx-clade-and-extensive-mosaicism-at-the-capsule-locus
#17
Abdulaziz Alqasim, Fleming Scheutz, Zhiyong Zong, Alan McNally
BACKGROUND: E.coli ST131 is a globally disseminated clone of multi-drug resistant E. coli responsible for that vast majority of global extra-intestinal E. coli infections. Recent global genomic epidemiological studies have highlighted the highly clonal nature of this group of bacteria, however there appears to be inconsistency in some phenotypes associated with the clone, in particular capsule types as determined by K-antigen testing both biochemically and by PCR. RESULTS: We performed improved quality assemblies on ten ST131 genomes previously sequenced by our group and compared them to a new reference genome sequence JJ1886 to identify the capsule loci across the drug-resistant clone H30Rx...
2014: BMC Genomics
https://www.readbyqxmd.com/read/24345742/the-epidemic-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-st131-is-driven-by-a-single-highly-pathogenic-subclone-h30-rx
#18
Lance B Price, James R Johnson, Maliha Aziz, Connie Clabots, Brian Johnston, Veronika Tchesnokova, Lora Nordstrom, Maria Billig, Sujay Chattopadhyay, Marc Stegger, Paal S Andersen, Talima Pearson, Kim Riddell, Peggy Rogers, Delia Scholes, Barbara Kahl, Paul Keim, Evgeni V Sokurenko
UNLABELLED: The Escherichia coli sequence type 131 (ST131) clone is notorious for extraintestinal infections, fluoroquinolone resistance, and extended-spectrum beta-lactamase (ESBL) production, attributable to a CTX-M-15-encoding mobile element. Here, we applied pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing to reconstruct the evolutionary history of the ST131 clone. PFGE-based cluster analyses suggested that both fluoroquinolone resistance and ESBL production had been acquired by multiple ST131 sublineages through independent genetic events...
2013: MBio
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