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https://www.readbyqxmd.com/read/29786855/speciation-of-bio-available-iodine-in-abalone-haliotis-discus-hannai-by-high-performance-liquid-chromatography-hyphenated-with-inductively-coupled-plasma-mass-spectrometry-using-an-in-vitro-method
#1
Han Sol Doh, Hyun Jin Park
Abalone is one of the most valuable marine products found in East Asia because it is rich in nutritious substances including iodine. In this study, the in vitro dialyzability approach was used to assess the bio-available iodine species in abalone. Iodide, iodate, 3-iodo-L-tyrosine (MIT), and 3,5-diiodo-L-tyrosine (DIT) were separated by high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). To assure the consistency, reliability, and accuracy of the data, the method was validated...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29775443/-b-type-natriuretic-peptide-as-a-marker-of-cardiac-dysfunction-in-children-with-chronic-kidney-disease
#2
Tomasz Drożdż, Przemko Kwinta, Zbigniew Kordon, Krystyna Sztefko, Andrzej Rudziński, Katarzyna Zachwieja, Monika Miklaszewska, Danuta Czarnecka, Dorota Drożdż
Left ventricular hypertrophy is the most common organ damage in children with chronic kidney disease (CKD). AIM: The aim of the study was to assess the usefulness of B-type natriuretic peptide (BNP) as a marker of heart injury in children with CKD. MATERIALS AND METHODS: We included 66 children (41 boys and 25 girls) aged 0.7 to 18.6 (median 11.6) years with CKD stage 1-5. The concentrations of urea, creatinine, cystatin C and BNP in blood serum were assessed, and the estimated glomerular filtration rate (eGFR) was calculated from the Schwartz and Filler formulas...
April 23, 2018: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29774568/effects-of-plasma-proteins-on-the-transport-and-surface-characteristics-of-polysulfone-polyethersulfone-and-asymmetric-cellulose-triacetate-high-flux-dialyzers
#3
Tae Ryung Kim, Mahsa Hadidi, Seyed Pouria Motevalian, Takashi Sunohara, Andrew L Zydney
Blood-membrane interactions can have a large impact on the performance of hemodialysis membranes, particularly for high flux membranes in which the membrane itself provides very low resistance to solute transport. The objective of this study was to examine the effects of exposure to serum on the solute clearance and convective sieving characteristics of high flux polysulfone (Optiflux F250NR), polyethersulfone (ELISIO-25H), and asymmetric cellulose triacetate (SOLACEA-25H) hemodialyzers using both vitamin B12 and a range of polydisperse dextrans...
May 17, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29772658/in-vitro-iron-bioavailability-of-brazilian-food-based-by-products
#4
Gabriela M Chiocchetti, Elisabete A De Nadai Fernandes, Anna A Wawer, Susan Fairweather-Tait, Tatiana Christides
Background : Iron deficiency is a public health problem in many low- and middle-income countries. Introduction of agro-industrial food by-products, as additional source of nutrients, could help alleviate this micronutrient deficiency, provide alternative sources of nutrients and calories in developed countries, and be a partial solution for disposal of agro-industry by-products. Methods : The aim of this study was to determine iron bioavailability of 5 by-products from Brazilian agro-industry (peels from cucumber, pumpkin, and jackfruit, cupuaçu seed peel, and rice bran), using the in vitro digestion/ Caco-2 cell model; with Caco-2 cell ferritin formation as a surrogate marker of iron bioavailability...
May 16, 2018: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29766001/production-of-recombinant-streptavidin-and-optimization-of-refolding-conditions-for-recovery-of-biological-activity
#5
Raoufe Modanloo Jouybari, Abdorrahim Sadeghi, Behzad Khansarinejad, Shabnam Sadoogh Abbasian, Hamid Abtahi
Background: Streptavidin is a protein produced by Streptomyces avidinii with strong biotin-binding ability. The non-covalent, yet strong bond between these two molecules has made it a preferable option in biological detection systems. Due to its extensive use, considerable attention is focused on streptavidin production by recombinant methods. Methods: In this study, streptavidin was expressed in Escherichia coli (E. coli) BL21 (DE3) pLysS cells and purified by affinity chromatography...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29764003/a-bioartificial-kidney-device-with-polarized-secretion-of-immune-modulators
#6
N V Chevtchik, M Mihajlovic, M Fedecostante, L Bolhuis-Versteeg, J Sastre Toraño, R Masereeuw, D Stamatialis
The accumulation of protein-bound toxins in dialyzed patients is strongly associated with their high morbidity and mortality. The bioartificial kidney device (BAK), containing proximal tubule epithelial cells (PTEC) seeded on functionalized synthetic hollow fiber membranes (HFM), may be a powerful solution for the active removal of those metabolites. In an earlier study, we developed an upscaled BAK containing conditionally immortalized human PTEC (ciPTEC) with functional organic cationic transporter 2 (OCT2)...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29750636/outcomes-of-very-elderly-treated-with-dialysis-for-acute-kidney-injury
#7
Ezra Gabbay, Yehudit Zangi, Alon Bnayah, Jawad Atrash, Itzchak Slotki, Linda Shavit
BACKGROUND: The factors that determine prognosis in elderly patients with dialysis-requiring acute kidney injury (AKI-D) is uncertain. The purpose of this study was to examine predictors of in-hospital mortality in these patients. MATERIALS AND METHODS: A retrospective, single-center study of hospitalized patients ≥ 70 years old with AKI-D. Clinical and demographic variables were compared between survivors and non-survivors, independent predictors of hospital mortality were identified by logistic regression...
May 11, 2018: Clinical Nephrology
https://www.readbyqxmd.com/read/29745004/heparin-free-renal-replacement-therapy-for-chronic-hemodialyzed-patients-at-high-risk-for-bleeding-a-comparison-of-on-line-predilution-hemodiafiltration-with-conventional-hemodialysis
#8
Vincent Brunot, Jean-Emmanuel Serre, Georges Mourad, Kada Klouche, Vincent Pernin
BACKGROUND: In chronic hemodialysis patients with high risk of bleeding, optimal anticoagulation of the extracorporeal circuit is challenging. Heparin-free hemodialysis (HD) with heparin-coated AN69ST dialyzer is now considered as a good option and recommended by experts. Predilutional hemodiafiltration (HDF) may represent also a feasible alternative but has been poorly investigated. In this study, our aim was to evaluate the performance of on-line automated predilution heparin-free HDF as compared to conventional heparin-free HD with a heparin-coated membrane...
May 10, 2018: Hemodialysis International
https://www.readbyqxmd.com/read/29734649/toxicological-assessment-of-a-lignin-core-nanoparticle-doped-with-silver-as-an-alternative-to-conventional-silver-core-nanoparticles
#9
Cassandra E Nix, Bryan J Harper, Cathryn G Conner, Alexander P Richter, Orlin D Velev, Stacey L Harper
Elevated levels of silver in the environment are anticipated with an increase in silver nanoparticle (AgNP) production and use in consumer products. To potentially reduce the burden of silver ion release from conventional solid core AgNPs, a lignin-core particle doped with silver ions and surface-stabilized with a polycationic electrolyte layer was engineered. Our objective was to determine whether any of the formulation components elicit toxicological responses using embryonic zebrafish. Ionic silver and free surface stabilizer were the most toxic constituents, although when associated separately or together with the lignin core particles, the toxicity of the formulations decreased significantly...
May 4, 2018: Antibiotics
https://www.readbyqxmd.com/read/29725932/light-chain-cast-nephropathy-practical-considerations-in-the-management-of-myeloma-kidney-what-we-know-and-what-the-future-may-hold
#10
REVIEW
Sandhya Manohar, Samih H Nasr, Nelson Leung
PURPOSE OF REVIEW: To update and evaluate the current knowledge on pathogenesis and management of light chain cast nephropathy. Light chain cast nephropathy (LCCN) is the leading cause of acute renal failure in patients with multiple myeloma and is currently recognized as a myeloma defining event. RECENT FINDINGS: The immunoglobulin free light chain plays an integral role in the pathogenesis of LCCN. The level of free light chain (FLC) in the blood and urine is directly associated with the risk of developing LCCN...
May 3, 2018: Current Hematologic Malignancy Reports
https://www.readbyqxmd.com/read/29723817/extracellular-matrix-coated-polyethersulfone-tpgs-hollow-fiber-membranes-showing-improved-biocompatibility-and-uremic-toxins-removal-for-bioartificial-kidney-application
#11
Akshay Modi, Surendra Kumar Verma, Jayesh Bellare
In this study, L-3, 4-dihydroxyphenylalanine and human collagen type IV were coated over the outer surface of the custom-made hollow fiber membranes (HFMs) with the objective of simultaneously improving biocompatibility leading to proliferation of human embryonic kidney cells-293 (HEK-293) and improving separation of uremic toxins, thereby making them suitable for bioartificial kidney application. Physicochemical characterization showed the development of coated HFMs, resulting in low hemolysis (0.25 ± 0...
April 24, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29704292/modulation-of-hippocampal-excitability-via-the-hydroxycarboxylic-acid-receptor-1
#12
Gabriel Herrera-López, Emilio J Galván
In addition to its prominent role as an energetic substrate in the brain, lactate is emerging as a signaling molecule capable of controlling neuronal excitability. The finding that the lactate-activated receptor (hydroxycarboxylic acid receptor 1; HCA1) is widely expressed in the brain opened up the possibility that lactate exerts modulation of neuronal activity via a transmembranal receptor-linked mechanism. Here, we show that lactate causes biphasic modulation of the intrinsic excitability of CA1 pyramidal cells...
April 27, 2018: Hippocampus
https://www.readbyqxmd.com/read/29666516/a-streamlined-isolation-method-and-the-autoxidation-profiles-of-tuna-myoglobin
#13
Mala Nurilmala, Hideki Ushio, Shugo Watabe, Yoshihiro Ochiai
Determination of the redox state of myoglobin (Mb) gives useful information for evaluating the quality of tuna meat. To attain this purpose, a fast streamlined method has been established basically based on preparative native gel electrophoresis to isolate Mb from the dark muscle of Pacific bluefin tuna. Crude Mb fraction was prepared from dark muscle by ammonium sulfate saturation fractionation and subsequently Mb was purified by preparative native gel electrophoresis under the isoelectric pH of the Mb, resulting in absorption (or trapping) of all the contaminating proteins in the gel...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29663430/effectiveness-of-a-new-single-needle-single-pump-dialysis-system-with-simultaneous-monitoring-of-dialysis-dose
#14
Wolfgang Bieser, Markus Welsch, Marten Jakob, Jörn Meibaum, David A Rodriguez, Henrik Wolff, Daniele Marcelli, Claudia Barth
To date, single-needle (SN) hemodialysis (HD) requires a dialysis machine equipped with two blood pumps-one controlling arterial blood flow (Qb) and one controlling venous Qb. B. Braun has developed an innovative single-pump SN HD system. Therefore, usability is improved by reducing complexity. The aim of this study was to compare dialysis parameters of the new single-pump SN HD system with a double-pump SN HD system available on the market (Fresenius Medical Care [FMC] 5008). In this two-armed crossover study, patients were randomized into two groups (B...
April 16, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29656848/the-discipline-of-epidemiology-engaging-in-the-full-breadth-of-population-health-science
#15
Harold I Feldman
This address was delivered by Harold I. Feldman, MD, MSCE, at the Annual Meeting of the American College of Epidemiology in New Orleans, Louisiana, on September 26, 2017. Dr. Feldman is George S. Pepper Professor of Public Health and Preventive Medicine, Professor of Epidemiology and Medicine, and Chair, Department of Biostatistics, Epidemiology, and Informatics at the Perelman School of Medicine at the University of Pennsylvania. He is recognized nationally and internationally for his expertise in kidney disease epidemiology...
March 21, 2018: Annals of Epidemiology
https://www.readbyqxmd.com/read/29650864/-a-case-of-home-care-for-heart-failure
#16
Haruhiko Tohyama
A female in her 80s with a history of cerebral infarction and atrial fibrillation had repeated hospitalizations because of heart failure caused by ischemic myocardiopathy. She lived alone and received home care and support for self-care with involvement of multiple providers, enabling early detection of disease exacerbation and subsequent intervention. A new ischemic event was managed with percutaneous coronary intervention. With deterioration of renal function, her response to diuretics decreased, and dialysis was initiated...
March 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29616582/kinetics-of-carnitine-concentration-after-switching-from-oral-administration-to-intravenous-injection-in-hemodialysis-patients
#17
Anna Suzuki, Yukinao Sakai, Kazumasa Hashimoto, Hirokazu Osawa, Shuichi Tsuruoka
Carnitine has high dialyzability and is often deficient in dialysis patients. This deficiency is treated by either intravenous (IV) or oral supplementation of carnitine. In this study, the mode of carnitine administration was changed from oral to IV in 17 hemodialysis (HD) patients, and the treatment was discontinued after 1 year. We found that the levels of total carnitine (TC), free-carnitine (FC), and acyl-carnitine (AC) significantly increased after 3 months of switching to IV administration (p < ...
November 2018: Renal Failure
https://www.readbyqxmd.com/read/29614232/formation-and-morphology-of-lipid-nanoparticles-containing-ionizable-cationic-lipids-and-sirna
#18
Jayesh A Kulkarni, Maria M Darjuan, Joanne E Mercer, Sam Chen, Roy van der Meel, Jenifer L Thewalt, Yuen Yi C Tam, Pieter R Cullis
Lipid nanoparticles (LNPs) containing short interfering RNA (LNP-siRNA) and optimized ionizable cationic lipids are now clinically validated systems for silencing disease-causing genes in hepatocytes following intravenous administration. However, the mechanism of formation and certain structural features of LNP-siRNA remain obscure. These systems are formed from lipid mixtures (cationic lipid, distearoylphosphatidylcholine, cholesterol, and PEG-lipid) dissolved in ethanol that is rapidly mixed with siRNA in aqueous buffer at a pH (pH 4) where the ionizable lipid is positively charged...
April 6, 2018: ACS Nano
https://www.readbyqxmd.com/read/29611341/outcome-and-predictors-of-renal-survival-in-patients-with-lupus-nephritis-comparison-between-cyclophosphamide-and-mycophenolate-mofetil
#19
Young Bin Joo, Young Mo Kang, Hyoun-Ah Kim, Chang-Hee Suh, Tae-Jong Kim, Yong-Wook Park, Jisoo Lee, Joo-Hyun Lee, Dae Hyun Yoo, Sang-Cheol Bae, Hye-Soon Lee, So-Young Bang
AIM: To compare renal outcomes between cyclophosphamide (CYC) and mycophenolate mofetil (MMF), and attempt to identify a predictor of renal survival. METHODS: A total of 99 patients with class III-V lupus nephritis (LN) and treated with CYC or MMF were enrolled. The remission rate and predictors of poor renal outcomes in LN were assessed. RESULTS: The mean age at LN diagnosis was 31.7 years. The baseline characteristics of the two groups were similar except for the chronicity index (3...
April 2, 2018: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29578402/chemotherapeutic-agents-eligible-for-prior-dosing-in-pancreatic-cancer-patients-requiring-hemodialysis-a-systematic-review
#20
Alexander Hann, Evelyn Nosalski, Patrick C Hermann, Jan Egger, Thomas Seufferlein, Frieder Keller
AIMS: New chemotherapeutic agents prolong survival of patients with pancreatic ductal adenocarcinoma (PDAC). Although their incidence is rising, patients with end-stage renal disease (ESRD) requiring hemodialysis (HD) are not included in the phase III trials evaluating the effects of these chemotherapies. Many experts recommend applying chemotherapy after HD using a reduced dose. Alternatively, the concept of prior dosing allows for the application of dialyzable chemotherapeutic drugs using a normal dose, with an HD followed shortly after to mimic normal renal function...
March 26, 2018: Clinical Nephrology
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